P Bastien

Summary

Country: France

Publications

  1. pmc Contamination management of broad-range or specific PCR: is there any difference?
    Patrick Bastien
    J Clin Microbiol 41:2272; author reply 2272. 2003
  2. ncbi request reprint Three years of multi-laboratory external quality control for the molecular detection of Toxoplasma gondii in amniotic fluid in France
    P Bastien
    Laboratoire de Parasitologie Mycologie, Centre hospitalier universitaire CHU, Universite Montpellier I, Montpellier, France
    Clin Microbiol Infect 13:430-3. 2007
  3. pmc Quantitative real-time PCR is not more sensitive than "conventional" PCR
    Patrick Bastien
    University of Montpellier 1 Centre Hospitalier Universitaire de Montpellier, Laboratory of Parasitology Mycology, Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Montpellier, France
    J Clin Microbiol 46:1897-900. 2008
  4. pmc Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood
    L Lachaud
    , Centre Hospitalier Universitaire, , Montpellier, France
    J Clin Microbiol 39:613-7. 2001
  5. ncbi request reprint Surveillance of leishmaniases in France, 1999 to 2012
    L Lachaud
    Centre Hospitalier Universitaire Academic Hospital Centre University Montpellier 1, Laboratoire de Parasitologie Mycologie, Centre National de référence des leishmanioses, Montpellier, France
    Euro Surveill 18:20534. 2013
  6. pmc Haemoculture as a tool for diagnosing visceral leishmaniasis in HIV-negative and HIV-positive patients: interest for parasite identification
    J Dereure
    , , Montpellier, France
    Bull World Health Organ 76:203-6. 1998
  7. doi request reprint 'Active chronic visceral leishmaniasis' in HIV-1-infected patients demonstrated by biological and clinical long-term follow-up of 10 patients
    N Bourgeois
    Laboratory of Parasitology Mycology, University Hospital, Nimes, University Montpellier I, Montpellier, Nimes, France
    HIV Med 11:670-3. 2010
  8. doi request reprint Diversity and evolution of methods and practices for the molecular diagnosis of congenital toxoplasmosis in France: a 4-year survey
    Y Sterkers
    Centre Hospitalier Universitaire CHU of Montpellier University of Montpellier 1, Montpellier, France
    Clin Microbiol Infect 16:1594-602. 2010
  9. pmc Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues
    Elisabeth Chabbert
    Laboratoire de Parasitologie Mycologie, Centre Hospitalier Universitaire, Montpellier, France
    J Clin Microbiol 42:1719-22. 2004
  10. doi request reprint Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    C Cassagne
    Laboratoire de Parasitologie Mycologie, CHU Timone, UMR MD3 Aix Marseille University Marseille, Marseille, France
    Clin Microbiol Infect 20:551-7. 2014

Collaborators

Detail Information

Publications23

  1. pmc Contamination management of broad-range or specific PCR: is there any difference?
    Patrick Bastien
    J Clin Microbiol 41:2272; author reply 2272. 2003
  2. ncbi request reprint Three years of multi-laboratory external quality control for the molecular detection of Toxoplasma gondii in amniotic fluid in France
    P Bastien
    Laboratoire de Parasitologie Mycologie, Centre hospitalier universitaire CHU, Universite Montpellier I, Montpellier, France
    Clin Microbiol Infect 13:430-3. 2007
    ..This external quality assurance scheme provided the opportunity to improve laboratory practice and performance, and to increase communication among laboratories involved in making this diagnosis...
  3. pmc Quantitative real-time PCR is not more sensitive than "conventional" PCR
    Patrick Bastien
    University of Montpellier 1 Centre Hospitalier Universitaire de Montpellier, Laboratory of Parasitology Mycology, Pôle Biologie Moléculaire du Centre National de Référence de la Toxoplasmose, Montpellier, France
    J Clin Microbiol 46:1897-900. 2008
  4. pmc Comparison of various sample preparation methods for PCR diagnosis of visceral leishmaniasis using peripheral blood
    L Lachaud
    , Centre Hospitalier Universitaire, , Montpellier, France
    J Clin Microbiol 39:613-7. 2001
    ....
  5. ncbi request reprint Surveillance of leishmaniases in France, 1999 to 2012
    L Lachaud
    Centre Hospitalier Universitaire Academic Hospital Centre University Montpellier 1, Laboratoire de Parasitologie Mycologie, Centre National de référence des leishmanioses, Montpellier, France
    Euro Surveill 18:20534. 2013
    ..This national notification system allowed a better understanding of the incidence and distribution of the disease; it is also useful to assess the temporal-spatial evolution of the disease in France, which appears relatively stable...
  6. pmc Haemoculture as a tool for diagnosing visceral leishmaniasis in HIV-negative and HIV-positive patients: interest for parasite identification
    J Dereure
    , , Montpellier, France
    Bull World Health Organ 76:203-6. 1998
    ..For each patient, the same zymodeme was found in bone marrow and blood cultures, both at initial diagnosis and at follow-up...
  7. doi request reprint 'Active chronic visceral leishmaniasis' in HIV-1-infected patients demonstrated by biological and clinical long-term follow-up of 10 patients
    N Bourgeois
    Laboratory of Parasitology Mycology, University Hospital, Nimes, University Montpellier I, Montpellier, Nimes, France
    HIV Med 11:670-3. 2010
    ..The aim of the study was to describe a new evolutionary form of visceral leishmaniasis observed in immunocompromised patients...
  8. doi request reprint Diversity and evolution of methods and practices for the molecular diagnosis of congenital toxoplasmosis in France: a 4-year survey
    Y Sterkers
    Centre Hospitalier Universitaire CHU of Montpellier University of Montpellier 1, Montpellier, France
    Clin Microbiol Infect 16:1594-602. 2010
    ..The high diversity of methods and practices currently used emphasizes the need for external quality assessment of the performances of the molecular diagnostic methods...
  9. pmc Comparison of two widely used PCR primer systems for detection of toxoplasma in amniotic fluid, blood, and tissues
    Elisabeth Chabbert
    Laboratoire de Parasitologie Mycologie, Centre Hospitalier Universitaire, Montpellier, France
    J Clin Microbiol 42:1719-22. 2004
    ..Nevertheless, when clinical samples were tested, both methods significantly differed in their technical sensitivities and specificities. Only one method appeared adequate for samples containing blood or tissue...
  10. doi request reprint Identification of Leishmania at the species level with matrix-assisted laser desorption ionization time-of-flight mass spectrometry
    C Cassagne
    Laboratoire de Parasitologie Mycologie, CHU Timone, UMR MD3 Aix Marseille University Marseille, Marseille, France
    Clin Microbiol Infect 20:551-7. 2014
    ..399. MALDI-TOF MS is a promising approach, providing rapid and accurate identification of Leishmania from in vitro culture at the species level...
  11. ncbi request reprint A direct method for the chromosomal assignment of DNA markers in Leishmania
    P Wincker
    Laboratoire CNRS EP 613 Génome des Parasites, Parasitologie, Faculte de Medecine, Montpellier, France
    Gene 194:77-80. 1997
    ..This makes a series of three blots sufficient for the assignment of any new marker on a particular Leishmania chromosome...
  12. ncbi request reprint Value of two PCR methods for the diagnosis of canine visceral leishmaniasis and the detection of asymptomatic carriers
    L Lachaud
    Laboratoire de Parasitologie Mycologie et Centre National de Référence sur les Leishmanioses, Faculte de Medecine, Montpellier, France
    Parasitology 125:197-207. 2002
    ..A strategy best adapted for prevalence surveys might combine serology and highly sensitive PCR on peripheral blood...
  13. pmc Comparison of six PCR methods using peripheral blood for detection of canine visceral leishmaniasis
    Laurence Lachaud
    Laboratoire de Parasitologie Mycologie et Centre National de Référence sur les Leishmanioses, Faculte de Medecine, Montpellier, France
    J Clin Microbiol 40:210-5. 2002
    ..Our data allow the discussion of the advantages and drawbacks of highly sensitive versus moderately sensitive PCR methods in diagnosis and prevalence studies of CVL...
  14. doi request reprint Long-term monitoring of visceral leishmaniasis in patients with AIDS: relapse risk factors, value of polymerase chain reaction, and potential impact on secondary prophylaxis
    Nathalie Bourgeois
    Laboratoire de Parasitologie Mycologie et Centre National de Référence des Leishmania, Centre Hospitalier Universitaire, Montpellier, France
    J Acquir Immune Defic Syndr 48:13-9. 2008
    ....
  15. pmc Compared genomics of the strand switch region of Leishmania chromosome 1 reveal a novel genus-specific gene and conserved structural features and sequence motifs
    Jacques Puechberty
    CNRS Université Montpellier, Biologie Moleculaire, Biologie Cellulaire et Biodiversité des Protozoaires Parasites, Laboratoire de Parasitologie Mycologie, UFR Medecine, Montpellier, France
    BMC Genomics 8:57. 2007
    ..Here, we have used an inter-species compared genomics approach on this locus in order to try to identify conserved features or motifs indicative of a putative function...
  16. pmc Multicentric comparative analytical performance study for molecular detection of low amounts of Toxoplasma gondii from simulated specimens
    Yvon Sterkers
    Laboratoire de Parasitologie Mycologie, Centre Hospitalier Universitaire, 39, Avenue Charles Flahault, F 34295, Montpellier Cedex 5, France
    J Clin Microbiol 48:3216-22. 2010
    ..But, on the other hand, interlaboratory comparisons showed differences that appear independent of the target, primers, or technology and that hence rely essentially on proficiency and care in the optimization of PCR conditions...
  17. ncbi request reprint Molecular diagnosis of toxoplasmosis
    Patrick Bastien
    CNRS UMR 5093 Gènome des Protozoaires Parasites, Laboratoire de Parasitologie Mycologie, Centre Hospitalier Universitaire, Faculte de Medecine, 163 Rue A Broussonet, 34090 Montpellier, France
    Trans R Soc Trop Med Hyg 96:S205-15. 2002
    ..These drawbacks are discussed, together with the undoubted gain that PCR has brought to this difficult diagnosis...
  18. ncbi request reprint Chromosome fragmentation in leishmania
    Pascal Dubessay
    CNRS UMR5093 Génome et Biologie Moléculaire des Protozoaires Parasites, Laboratoire de Parasitologie Mycologie, Faculte de Medicine, Montpellier, France
    Methods Mol Biol 270:353-78. 2004
    ..Still, this powerful method has enriched our basic knowledge of chromosomal structure and maintenance...
  19. ncbi request reprint Strain typing in Leishmania donovani by using sequence-confirmed amplified region analysis
    Sylke Lewin
    Institute of Microbiology and Hygiene, Charite Campus Mitte, Dorotheenstrasse 96, D 10117 Berlin, Germany
    Int J Parasitol 32:1267-76. 2002
    ..They are co-dominant because they can detect all possible allele combinations in a diploid organism and may, therefore, be very useful for population genetic analysis in Leishmania...
  20. pmc The switch region on Leishmania major chromosome 1 is not required for mitotic stability or gene expression, but appears to be essential
    Pascal Dubessay
    CNRS UMR5093 Génome et Biologie Moléculaire des Protozoaires Parasites, Laboratoire de Parasitologie Mycologie, Faculte de Medecine, 163 rue Auguste Broussonet, F 34090 Montpellier, France
    Nucleic Acids Res 30:3692-7. 2002
    ..This result suggests that the presence of this 1.6-kb sequence is not necessary for the expression of all genes on chromosome 1...
  21. ncbi request reprint Mitotic stability of a coding DNA sequence-free version of Leishmania major chromosome 1 generated by targeted chromosome fragmentation
    Pascal Dubessay
    CNRS UMR5093 Génome et Biologie Moléculaire des Protozoaires Parasites, Laboratoire de Parasitologie Mycologie, Faculte de Medecine, 163 Rue A Broussonet, F 34090 Montpellier, France
    Gene 289:151-9. 2002
    ....
  22. ncbi request reprint Visceral leishmaniasis in organ transplant recipients: 11 new cases and a review of the literature
    Didier Basset
    Laboratoire de Parasitologie Mycologie and Centre National de Référence des Leishmania, CHU de Montpellier, 163, Rue Auguste Broussonet, 34090 Montpellier, France
    Microbes Infect 7:1370-5. 2005
    ..Treatment using either antimonials or amphotericine B gave similar cure rates of around 80% of the cases. But toxicity was higher for antimonials. Relapses occurred in 14.3%...
  23. ncbi request reprint Two case reports of symptomatic visceral leishmaniasis in AIDS patients concomitant with immune reconstitution due to antiretroviral therapy
    Antoine Berry
    Service des Maladies Infectieuses et Tropicales, Hopital Gui de Chauliac, Centre Hospitalier Universitaire, 34295 Montpellier Cedex 5, France
    Scand J Infect Dis 36:225-7. 2004
    ..The report raises the question whether a rapid immune reconstitution may convert a latent visceral leishmaniasis into a symptomatic one...