Affiliation: Helsinki University of Technology
- In silico evidence for functional specialization after genome duplication in yeastOssi Turunen
Department of Biotechnology and Chemical Technology, Helsinki University of Technology, Espoo, Finland
FEMS Yeast Res 9:16-31. 2009..Our results indicate that subfunctionalization and even neofunctionalization has occurred along with degenerative evolution, in which unneeded functions were destroyed by mutations...
- Protein engineering: opportunities and challengesMatti Leisola
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Espoo, Finland
Appl Microbiol Biotechnol 75:1225-32. 2007..In the long run, random methods cannot replace insight in constructing life-like proteins. For the near future, however, in enzyme development, we need to rely on a combination of both...
- Engineering of multiple arginines into the Ser/Thr surface of Trichoderma reesei endo-1,4-beta-xylanase II increases the thermotolerance and shifts the pH optimum towards alkaline pHOssi Turunen
Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT, Finland
Protein Eng 15:141-5. 2002..The stabilizing effect of arginines at high temperatures was seen clearly only when five arginines were introduced into the Ser/Thr surface...
- Engineering the thermostability of Trichoderma reesei endo-1,4-beta-xylanase II by combination of disulphide bridgesHairong Xiong
Laboratory of Bioprocess Engineering, Helsinki University of Technology, P O Box 6100, 02015 HUT, Helsinki, Finland
Extremophiles 8:393-400. 2004..The combination of disulphide bridges enhanced significantly the pH-dependent stability in a wide pH range...
- Engineering the thermotolerance and pH optimum of family 11 xylanases by site-directed mutagenesisOssi Turunen
Laboratory of Bioprocess Engineering, Helsinki University of Technology, Finland
Methods Enzymol 388:156-67. 2004
- Engineering the substrate specificity of xylose isomeraseJohanna Karimäki
Laboratory of Bioprocess Engineering, Helsinki University of Technology, PO Box 6100, 02015 HUT and Department of Chemistry, University of Joensuu, PO Box 111, 80101 Joensuu, Finland
Protein Eng Des Sel 17:861-9. 2004..It improved 3-fold the catalytic efficiency of XI with L-arabinose; this increase was seen in both Km and kcat. This study showed that it is possible to engineer the substrate specificity of XI...
- Effect of glycosylation and additional domains on the thermostability of a family 10 xylanase produced by Thermopolyspora flexuosaSasikala Anbarasan
Department of Biotechnology and Chemical Technology, Aalto University, P O Box 6100, 02015 TKK, Espoo, Finland
Appl Environ Microbiol 76:356-60. 2010..In contrast, glycosylation at Asn26, located in an exposed loop, decreased the thermostability of the xylanase. The presence of a substrate increased stability mainly at low pH...