S Kirkeby

Summary

Affiliation: University of Copenhagen
Country: Denmark

Publications

  1. ncbi request reprint Comparison of the binding properties of the mushroom Marasmius oreades lectin and Griffonia simplicifolia I-B isolectin to alphagalactosyl carbohydrate antigens in the surface phase
    Svend Kirkeby
    Department of Oral Medicine, Dental School, The Panum Institute, University of Copenhagen, Nørre Allé 20, 2200 N Copenhagen, Denmark
    Xenotransplantation 11:254-61. 2004
  2. ncbi request reprint Quantitative immunohistochemistry of fluorescence labelled probes using low-cost software
    Svend Kirkeby
    Department of Oral Medicine, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, 20 Nørre Allé, DK 2200 Copenhagen, Denmark
    J Immunol Methods 301:102-13. 2005
  3. ncbi request reprint Analyses of Pseudomonas aeruginosa lectin binding to alpha-galactosylated glycans
    S Kirkeby
    Department of Oral Medicine and Microbiology, Dental School, The Panum Institute, University of Copenhagen, Nørre Allé 20, Copenhagen, 2200, Denmark
    Curr Microbiol 50:309-13. 2005
  4. ncbi request reprint The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink
    Svend Kirkeby
    Department of Oral Medicine, Dental School, University of Copenhagen, Nørre Allé 20, DK 2200 Copenhagen, Denmark
    Microbes Infect 9:566-73. 2007
  5. doi request reprint Distribution of the alphaGal- and the non-alphaGal T-antigens in the pig kidney: potential targets for rejection in pig-to-man xenotransplantation
    Svend Kirkeby
    Department of Oral Medicine, The Panum Institute, School of Dentistry, University of Copenhagen, Copenhagen N, Denmark
    Immunol Cell Biol 86:363-71. 2008
  6. ncbi request reprint Solid phase measurements of antibody and lectin binding to xenogenic carbohydrate antigens
    Svend Kirkeby
    Institute of Oral Medicine, The Panum Institute, Dental School, University of Copenhagen, 2200 Copenhagen N, Denmark
    Clin Biochem 37:36-41. 2004
  7. doi request reprint Asthma induction in mice leads to appearance of alpha2-3- and alpha2-6-linked sialic acid residues in respiratory goblet-like cells
    Svend Kirkeby
    Dental School, University of Copenhagen, Copenhagen, Denmark
    Virchows Arch 453:283-90. 2008
  8. ncbi request reprint The galactophilic lectin (PA-IL, gene LecA) from Pseudomonas aeruginosa. Its binding requirements and the localization of lectin receptors in various mouse tissues
    Svend Kirkeby
    Department of Oral Medicine, Dental School, University of Copenhagen, Nørre Allé 20, DK 2200 Copenhagen, Denmark
    Microb Pathog 40:191-7. 2006
  9. doi request reprint Infection with human H1N1 influenza virus affects the expression of sialic acids of metaplastic mucous cells in the ferret airways
    S Kirkeby
    Dental School, Faculty of Health Sciences, University of Copenhagen, Denmark
    Virus Res 144:225-32. 2009
  10. ncbi request reprint Binding of Griffonia simplicifolia 1 isolectin B4 (GS1 B4) to alpha-galactose antigens
    S Kirkeby
    Department of Oral Function and Physiology, The Panum Institute, Nørre Allé 20, Copenhagen 2200 N, Denmark
    Immunol Cell Biol 79:121-7. 2001

Collaborators

Detail Information

Publications27

  1. ncbi request reprint Comparison of the binding properties of the mushroom Marasmius oreades lectin and Griffonia simplicifolia I-B isolectin to alphagalactosyl carbohydrate antigens in the surface phase
    Svend Kirkeby
    Department of Oral Medicine, Dental School, The Panum Institute, University of Copenhagen, Nørre Allé 20, 2200 N Copenhagen, Denmark
    Xenotransplantation 11:254-61. 2004
    ....
  2. ncbi request reprint Quantitative immunohistochemistry of fluorescence labelled probes using low-cost software
    Svend Kirkeby
    Department of Oral Medicine, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, 20 Nørre Allé, DK 2200 Copenhagen, Denmark
    J Immunol Methods 301:102-13. 2005
    ..With the tools described in the present report it is thus possible also to optimize the topical immunohistochemical protocol in order to quantify the fluorescence signal...
  3. ncbi request reprint Analyses of Pseudomonas aeruginosa lectin binding to alpha-galactosylated glycans
    S Kirkeby
    Department of Oral Medicine and Microbiology, Dental School, The Panum Institute, University of Copenhagen, Nørre Allé 20, Copenhagen, 2200, Denmark
    Curr Microbiol 50:309-13. 2005
    ..These observations on PA-IL binding could be of importance both to determine infection foci in P. aeruginosa-mediated vacuities and to determine its role for pancreatic involvement in cystic fibrosis...
  4. ncbi request reprint The mink as an animal model for Pseudomonas aeruginosa adhesion: binding of the bacterial lectins (PA-IL and PA-IIL) to neoglycoproteins and to sections of pancreas and lung tissues from healthy mink
    Svend Kirkeby
    Department of Oral Medicine, Dental School, University of Copenhagen, Nørre Allé 20, DK 2200 Copenhagen, Denmark
    Microbes Infect 9:566-73. 2007
    ..Judging from the results in the present paper the mink should be considered a suitable model to study P. aeruginosa adherence...
  5. doi request reprint Distribution of the alphaGal- and the non-alphaGal T-antigens in the pig kidney: potential targets for rejection in pig-to-man xenotransplantation
    Svend Kirkeby
    Department of Oral Medicine, The Panum Institute, School of Dentistry, University of Copenhagen, Copenhagen N, Denmark
    Immunol Cell Biol 86:363-71. 2008
    ..The study thus proves a complex spatial distribution of carbohydrate antigens relevant for xenotransplantation of pig kidney...
  6. ncbi request reprint Solid phase measurements of antibody and lectin binding to xenogenic carbohydrate antigens
    Svend Kirkeby
    Institute of Oral Medicine, The Panum Institute, Dental School, University of Copenhagen, 2200 Copenhagen N, Denmark
    Clin Biochem 37:36-41. 2004
    ..In future pig-to-man xenotransplantation it is important to master tools that identify potentially xenogenic alphagalactose (Galalpha) antigens in the doner tissue...
  7. doi request reprint Asthma induction in mice leads to appearance of alpha2-3- and alpha2-6-linked sialic acid residues in respiratory goblet-like cells
    Svend Kirkeby
    Dental School, University of Copenhagen, Copenhagen, Denmark
    Virchows Arch 453:283-90. 2008
    ..Instead, an antibody recognizing a disialoganglioside, stained large mononuclear cells in the submucosa, indicating a difference in sialylation between goblet cells in the intestine and goblet-like cells developed from Clara cells...
  8. ncbi request reprint The galactophilic lectin (PA-IL, gene LecA) from Pseudomonas aeruginosa. Its binding requirements and the localization of lectin receptors in various mouse tissues
    Svend Kirkeby
    Department of Oral Medicine, Dental School, University of Copenhagen, Nørre Allé 20, DK 2200 Copenhagen, Denmark
    Microb Pathog 40:191-7. 2006
    ..Finally, lectin staining of the basement membrane of the acini in the exocrine pancreas suggests the presence of Galalpha1-3Gal epitopes in WT mice basement membranes that are not detected by the P. aeruginosa lectin...
  9. doi request reprint Infection with human H1N1 influenza virus affects the expression of sialic acids of metaplastic mucous cells in the ferret airways
    S Kirkeby
    Dental School, Faculty of Health Sciences, University of Copenhagen, Denmark
    Virus Res 144:225-32. 2009
    ..The submucosal tracheal glands in these animals showed increased expression of both Sialyl alpha 2-3 and Sialyl alpha 2-6 epitopes...
  10. ncbi request reprint Binding of Griffonia simplicifolia 1 isolectin B4 (GS1 B4) to alpha-galactose antigens
    S Kirkeby
    Department of Oral Function and Physiology, The Panum Institute, Nørre Allé 20, Copenhagen 2200 N, Denmark
    Immunol Cell Biol 79:121-7. 2001
    ..In Western blots of isolated proteins from pig muscle at least three bands were strongly stained after incubation with lectin...
  11. ncbi request reprint Localized agglutinin staining in muscle capillaries from normal and very old atrophic human muscle using winged bean (Psophocarpus tetragonolobus) lectin
    S Kirkeby
    Department of Oral Function and Physiology, Faculty of Health Sciences, University of Copenhagen, Denmark Svend
    Histochem Cell Biol 107:31-7. 1997
    ..The study demonstrates the usefulness of WBA as a marker of capillaries in human muscle...
  12. ncbi request reprint Binding properties of the galactose-detecting lectin Pseudomonas aeruginosa agglutinin (PA-IL) to skeletal muscle fibres. Quantitative precipitation and precipitation inhibition assays
    S Kirkeby
    Department of Oral Function and Physiology, Faculty of Health Sciences, University of Copenhagen, Denmark
    Histochem J 31:485-93. 1999
    ..It may therefore be proposed that glycoconjugates carrying terminal Galalpha1 show affinity for PA-IL and are located to the sarcoplasmic reticulum...
  13. ncbi request reprint Immunohistochemistry of the intercellular matrix components and the epithelio-mesenchymal junction of the human tooth germ
    C Garbarsch
    Institute of Medical Anatomy A, Panum Institute, University of Copenhagen, Denmark
    Histochem J 26:110-8. 1994
    ..The results of this study have supported the assumption that the lamina basalis ameloblastica is a basement membrane, and have lead to the suggestion that ameloblasts are producers of fibronectin or a fibronectin-like substance...
  14. ncbi request reprint Galalpha1-->4Gal-glycans are expressed on myofibrillar associated proteins
    S Kirkeby
    Department of Oral Function and Physiology, Faculty of Health Sciences, University of Copenhagen, 20 Norre Alle, DK 2200 Copenhagen N, Denmark
    Cell Tissue Res 293:285-91. 1998
    ..This study indicates that glycans carrying Galalpha1-4Galbeta1- epitopes are expressed on myofibrillar associated proteins...
  15. ncbi request reprint Effect of saliva composition on experimental root caries
    A Bardow
    Department of Oral Medicine, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Nørre Allé 20, DK 2200 Copenhagen N, Denmark
    Caries Res 39:71-7. 2005
    ..Furthermore, high UWS concentrations of phosphate, protein, and amylase were caries-protective...
  16. ncbi request reprint Histochemical studies of the masseter, the temporal and small zygomaticomandibular, and the temporomandibular masticatory muscles from aged male and female humans. Fiber types and myosin isoforms
    S Kirkeby
    School of Dentistry, Copenhagen Gerontological Oral Health Research Center, Dept of Oral Function and Physiology, The Panum Institute, Denmark
    Cranio 19:174-82. 2001
    ..Neither did the fibers show accordance in their content of adult slow and fast myosin heavy chains and in their content of neonatal myosin heavy chain...
  17. doi request reprint Individual variations in protective effects of experimentally formed salivary pellicles
    M Bruvo
    Department of Oral Microbiology, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Caries Res 43:163-70. 2009
    ..We conclude that individual variations in experimental pellicle protection against erosive challenges exist and that such variations appear not to be due to differences in a single protein component...
  18. ncbi request reprint Non-specific esterases in partly mineralized bovine enamel
    D Moe
    Institute of Biochemistry, Royal Dental College, Copenhagen, Denmark
    Acta Odontol Scand 48:327-32. 1990
    ..The enzymes showed highest activity at pH 6.5-7.5. In sections a strong reaction was observed in the secretory ameloblasts. The esterases may be proteolytic enzymes that participate in the degradation of the matrix proteins...
  19. doi request reprint Bacterial adherence in otitis media: determination of N-acetylgalactosamine (GalNAc) residues in the submucosal glands and surface epithelium of the normal and diseased Eustachian tube
    S Kirkeby
    Department of Oral Medicine, Dental School, Panum Institute, University of Copenhagen, Copenhagen, Denmark
    Microb Pathog 51:48-57. 2011
    ..N-acetylgalactosamine (GalNAc) is an important constituent of mucins and GalNAc containing sugar residues seem to be essential for initial adherence of respiratory bacteria to the surface of epithelial cells...
  20. ncbi request reprint Carboxylic ester hydrolases in mitochondria from rat skeletal muscle
    S Kirkeby
    Royal Dental College, Copenhagen, Denmark
    Histochem J 22:95-101. 1990
    ..After freezing, thawing and high speed centrifugation most of the alpha-naphthyl acetate splitting enzymes were found in the supernatant, indicating that the enzymes are loosely bound to mitochondrial membranes...
  21. ncbi request reprint Sirius red and acid fuchsin staining mechanisms
    L F Nielsen
    Faculty of Health Sciences, University of Copenhagen, The Panum Institute, Denmark
    Biotech Histochem 73:71-7. 1998
    ..The staining seems not to be selective for collagen, and one must account for this when quantitative conclusions are drawn from collagen methods using these stains...
  22. doi request reprint Chemical modification of carbohydrates in tissue sections may unmask mucin antigens
    S Kirkeby
    Department of Oral Medicine, Dental School, Panum Institute, University of Copenhagen, Denmark
    Biotech Histochem 88:19-26. 2013
    ..It appears that chemical modification can either destroy some antigen binding sites or unmask cryptic antigen binding sites in the mucin molecule and thereby make them accessible for immunohistochemical detection...
  23. ncbi request reprint Aging affects different human muscles in various ways. An image analysis of the histomorphometric characteristics of fiber types in human masseter and vastus lateralis muscles from young adults and the very old
    S Kirkeby
    Department of Oral Function and Physiology, Faculty of Health Sciences, University of Copenhagen, Denmark
    Histol Histopathol 15:61-71. 2000
    ..Histomorphometric measurements show that aging affects different human muscles in various ways...
  24. ncbi request reprint The resistance of delayed xenograft rejection to alpha(1,3)-galactosyltransferase gene inactivation and CD4 depletion in a mouse-to-rat model
    Alastair B Hansen
    Department of Pathology, Herlev Hospital, University of Copenhagen, Herlev, Denmark
    APMIS 111:1019-26. 2003
    ..However, other Galalpha 1 epitopes and antibodies may play a role during DXR. Further studies are needed to elucidate the precise pathways leading to DXR...
  25. ncbi request reprint Lectin interactions with alpha-galactosylated xenoantigens
    Svend Kirkeby
    Department of Oral Function and Physiology, Dental School, Copenhagen, Denmark
    Xenotransplantation 9:260-7. 2002
    ..When lectins are used for recognition and investigation of natural Galalpha-antigens, we propose that GS1 B4 and MPA should accompany each other...
  26. ncbi request reprint Xenotransplantation--state of the art
    Axel Kornerup Hansen
    Center of Bioethics and Risk Assessment, Royal Veterinary and Agricultural University, Bülowsvej 15, DK 1870 Frederiksberg C, Denmark
    Acta Vet Scand Suppl 99:7-12. 2004
  27. doi request reprint Aggression in cataract-bearing alpha-1,3-galactosyltransferase knockout mice
    Dorte Bratbo Sørensen
    Centre for Bioethics and Risk Assessment, Division of Laboratory Animal Science and Welfare, Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, Groennegaardsvej 15, DK 1870 Frederiksberg C, Denmark
    Lab Anim 42:34-44. 2008
    ..Apart from this, the agonistic behaviour was not influenced by genotype. Neither did the genotype affect anxiety or perception of light...