Barbara Ann Halkier

Summary

Affiliation: The Royal Veterinary and Agricultural University
Country: Denmark

Publications

  1. pmc USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, Institute of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
    Nucleic Acids Res 35:e55. 2007
  2. pmc Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
    Hussam H Nour-Eldin
    Plant Biochemistry Laboratory, Center for Molecular Plant Physiology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    Nucleic Acids Res 34:e122. 2006
  3. ncbi request reprint Biology and biochemistry of glucosinolates
    Barbara Ann Halkier
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, DK 1871 Frederiksberg C, Denmark
    Annu Rev Plant Biol 57:303-33. 2006
  4. pmc Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis
    Tonni Grube Andersen
    DynaMo Centre of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark
    Plant Cell 25:3133-45. 2013
  5. pmc Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis
    Fernando Geu-Flores
    Section for Molecular Plant Biology, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark
    Plant Cell 23:2456-69. 2011
  6. doi request reprint Towards engineering glucosinolates into non-cruciferous plants
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, VKR Research Centre Pro Active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark
    Planta 229:261-70. 2009
  7. doi request reprint Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform
    Michael Dalgaard Mikkelsen
    University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology and Biotechnology, Molecular Plant Biology, VKR Research Centre for Pro Active Plants, 40 Thorvaldsensvej, Frederiksberg C, Copenhagen, Denmark
    Metab Eng 14:104-11. 2012
  8. pmc A complex interplay of three R2R3 MYB transcription factors determines the profile of aliphatic glucosinolates in Arabidopsis
    Ida Elken Sønderby
    Plant Biochemistry Laboratory, Villum Kann Rasmussen Research Centre for Pro Active Plants, and Plant Biochemistry Laboratory, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, Copenhagen University, 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 153:348-63. 2010
  9. pmc A Functional EXXEK Motif is Essential for Proton Coupling and Active Glucosinolate Transport by NPF2.11
    Morten Egevang Jørgensen
    DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark
    Plant Cell Physiol 56:2340-50. 2015
  10. pmc Elucidating the role of transport processes in leaf glucosinolate distribution
    Svend Roesen Madsen
    Danish National Research Foundation Center for Dynamic Molecular Interactions S R M, H H N E, B A H and Department of Plant and Environmental Sciences, Faculty of Science S R M, C E O, H H N E, B A H, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
    Plant Physiol 166:1450-62. 2014

Collaborators

  • Danielj Kliebenstein
  • Fernando Geu-Flores
  • Jonathan Gershenzon
  • Ingo Dreyer
  • Jing Li
  • Morten H H Nørholm
  • Christoph Bottcher
  • Rainer Hedrich
  • Hussam Hassan Nour-Eldin
  • Michael Dalgaard Mikkelsen
  • Carl Erik Olsen
  • Morten Egevang Jørgensen
  • Svend Roesen Madsen
  • Tonni Grube Andersen
  • Meike Burow
  • Christoph Crocoll
  • Bjarne Gram Hansen
  • Ida Elken Sønderby
  • Daniel Vik
  • Deyang Xu
  • Michael Reichelt
  • Bent Larsen Petersen
  • Dietmar Geiger
  • Peter Naur
  • Heather C Rowe
  • Carla Ticconi
  • Adam M Wentzell
  • Günter Brader
  • Hussam H Nour-Eldin
  • Titima Tantikanjana
  • Franziska S Hanschen
  • Monika Schreiner
  • Sebastian J Nintemann
  • Nadia Mirza
  • Katja Witzel
  • Osman Mirza
  • Grit Kunert
  • Victoria Louise Fuller
  • Line Due Buron
  • Uffe Hasbro Mortensen
  • Bo Salomonsen
  • Henrik Bjørn Nielsen
  • Victoria L Fuller
  • Majse Nafisi
  • Nanna Bjarnholt
  • E Tapio Palva
  • Bjarne G Hansen
  • Jacob K Jensen
  • Mumtaz Hussain
  • Venkatesan Sundaresan
  • Erich Glawischnig
  • Søren Bak
  • Anders Bøgh Jensen
  • Erik Andreasson
  • Hasse Rasmussen
  • Sixue Chen
  • Carsten Hørslev Hansen

Detail Information

Publications32

  1. pmc USER fusion: a rapid and efficient method for simultaneous fusion and cloning of multiple PCR products
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, Institute of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Thorvaldsensvej 40, Frederiksberg C, Denmark
    Nucleic Acids Res 35:e55. 2007
    ..We expect this method to replace overlapping PCR and the use of type IIS restriction enzymes in many of their applications...
  2. pmc Advancing uracil-excision based cloning towards an ideal technique for cloning PCR fragments
    Hussam H Nour-Eldin
    Plant Biochemistry Laboratory, Center for Molecular Plant Physiology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    Nucleic Acids Res 34:e122. 2006
    ..A comprehensive set of vectors has been constructed covering a wide range of different applications and their functionality has been confirmed...
  3. ncbi request reprint Biology and biochemistry of glucosinolates
    Barbara Ann Halkier
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, DK 1871 Frederiksberg C, Denmark
    Annu Rev Plant Biol 57:303-33. 2006
    ..This may enable us to more fully exploit the potential of these compounds in agriculture and medicine...
  4. pmc Integration of biosynthesis and long-distance transport establish organ-specific glucosinolate profiles in vegetative Arabidopsis
    Tonni Grube Andersen
    DynaMo Centre of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark
    Plant Cell 25:3133-45. 2013
    ....
  5. pmc Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis
    Fernando Geu-Flores
    Section for Molecular Plant Biology, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 1871 Frederiksberg, Denmark
    Plant Cell 23:2456-69. 2011
    ....
  6. doi request reprint Towards engineering glucosinolates into non-cruciferous plants
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, VKR Research Centre Pro Active Plants, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark
    Planta 229:261-70. 2009
    ..In summary, we transferred the last three steps of the benzylglucosinolate pathway to tobacco as a first step towards engineering glucosinolates into non-cruciferous plants...
  7. doi request reprint Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform
    Michael Dalgaard Mikkelsen
    University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology and Biotechnology, Molecular Plant Biology, VKR Research Centre for Pro Active Plants, 40 Thorvaldsensvej, Frederiksberg C, Copenhagen, Denmark
    Metab Eng 14:104-11. 2012
    ..Production of indolylglucosinolate serves as a proof-of-concept for our expression platform, and provides a basis for large-scale microbial production of specific glucosinolates for the benefit of human health...
  8. pmc A complex interplay of three R2R3 MYB transcription factors determines the profile of aliphatic glucosinolates in Arabidopsis
    Ida Elken Sønderby
    Plant Biochemistry Laboratory, Villum Kann Rasmussen Research Centre for Pro Active Plants, and Plant Biochemistry Laboratory, Department of Plant Biology and Biotechnology, Faculty of Life Sciences, Copenhagen University, 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 153:348-63. 2010
    ..Similar regulatory networks might exist in other secondary pathways...
  9. pmc A Functional EXXEK Motif is Essential for Proton Coupling and Active Glucosinolate Transport by NPF2.11
    Morten Egevang Jørgensen
    DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark
    Plant Cell Physiol 56:2340-50. 2015
    ..We hypothesize that introducing a charge at X1 and X2 interferes with the function of the conserved glutamate and lysine residues of the E1X1X2E2K motif and affects the mechanism behind proton coupling. ..
  10. pmc Elucidating the role of transport processes in leaf glucosinolate distribution
    Svend Roesen Madsen
    Danish National Research Foundation Center for Dynamic Molecular Interactions S R M, H H N E, B A H and Department of Plant and Environmental Sciences, Faculty of Science S R M, C E O, H H N E, B A H, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
    Plant Physiol 166:1450-62. 2014
    ....
  11. doi request reprint The emerging field of transport engineering of plant specialized metabolites
    Hussam Hassan Nour-Eldin
    DynaMo Centre of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
    Curr Opin Biotechnol 24:263-70. 2013
    ..As glucosinolates are specialized metabolites of the model plant Arabidopsis, glucosinolate transport processes emerge as a model system for studying transport of specialized metabolites...
  12. doi request reprint NRT/PTR transporters are essential for translocation of glucosinolate defence compounds to seeds
    Hussam Hassan Nour-Eldin
    Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871Frederiksberg C, Denmark
    Nature 488:531-4. 2012
    ..Identification of the glucosinolate transporters has agricultural potential as a means to control allocation of defence compounds in a tissue-specific manner...
  13. doi request reprint Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2
    Michael Dalgaard Mikkelsen
    VKR Research Centre Pro Active Plants, Department of Plant Biology and Biotechnology, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark
    Planta 229:1209-17. 2009
    ..The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism...
  14. doi request reprint Production of the cancer-preventive glucoraphanin in tobacco
    Michael Dalgaard Mikkelsen
    University of Copenhagen, Faculty of Life Sciences, Department of Plant Biology, Plant Biochemistry Laboratory, VKR Research Centre for Pro Active Plants, 40 Thorvaldsensvej, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Mol Plant 3:751-9. 2010
    ..Demonstrating the production of the high-value glucoraphanin in a heterologous host has great potential in the food and medicinal industry as a means to generate a stable, rich source of glucoraphanin for the benefit of human health...
  15. pmc Optimization of Engineered Production of the Glucoraphanin Precursor Dihomomethionine in Nicotiana benthamiana
    Christoph Crocoll
    DNRF Center DynaMo, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark Copenhagen Plant Science Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Denmark
    Front Bioeng Biotechnol 4:14. 2016
    ..2010). The increased DHM production without increasing leucine-derived side-product levels provides new metabolic engineering strategies for improved glucoraphanin production in a heterologous host. ..
  16. pmc Subclade of flavin-monooxygenases involved in aliphatic glucosinolate biosynthesis
    Jing Li
    Plant Biochemistry Laboratory, Department of Plant Biology and Villum Kann Rasmussen Research Centre for Pro Active Plants, Faculty of Life Sciences, University of Copenhagen, DK 1871 Frederiksberg C, Denmark
    Plant Physiol 148:1721-33. 2008
    ....
  17. pmc CB5C affects the glucosinolate profile in Arabidopsis thaliana
    Daniel Vik
    a Department of Plant and Environmental Sciences, DynaMo Center of Excellence and Copenhagen Plant Science Center, University of Copenhagen, Denmark
    Plant Signal Behav 11:e1160189. 2016
    ..Furthermore, the mutants differed in their biomass response to methyl jasmonate treatment. Thereby, our results highlight the varying effects of T-DNA insertion sites, as the 2 analyzed alleles show different phenotypes. ..
  18. doi request reprint A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
    Morten Egevang Jørgensen
    DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871, Frederiksberg C, Denmark
    Methods Mol Biol 1405:99-107. 2016
    ..However, a large content of yolk platelets in the oocyte cytoplasm makes this a challenging task. Here we report a method for fast and easy, semiquantitative Western blot analysis of proteins heterologously expressed in Xenopus oocytes. ..
  19. doi request reprint Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
    Svend Roesen Madsen
    DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark
    Methods Mol Biol 1405:35-42. 2016
    ..Due to the fragile nature of leaf tissues, it is a challenge to obtain apoplastic fluids from leaves. Here, methods to collect apoplastic washing fluid and guttation fluid from Arabidopsis thaliana leaves are described. ..
  20. pmc Feeding on Leaves of the Glucosinolate Transporter Mutant gtr1gtr2 Reduces Fitness of Myzus persicae
    Svend Roesen Madsen
    DynaMo Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 40 Thorvaldsensvej, DK 1871, Frederiksberg C, Denmark
    J Chem Ecol 41:975-84. 2015
    ..Our data suggest that higher glucosinolate content in tissues surrounding the phloem of the double transporter mutant may play a role in reducing aphid performance on this genotype. ..
  21. doi request reprint Transport of defense compounds from source to sink: lessons learned from glucosinolates
    Morten Egevang Jørgensen
    DynaMo, DNRF Center of Excellence, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark
    Trends Plant Sci 20:508-14. 2015
    ..We focus on lessons learned from glucosinolate transport that may apply to transport of other defense compounds and discuss future avenues in the emerging field of defense compound transport...
  22. doi request reprint Glucosinolate engineering identifies a gamma-glutamyl peptidase
    Fernando Geu-Flores
    Plant Biochemistry Laboratory, Department of Plant Biology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark
    Nat Chem Biol 5:575-7. 2009
    ....
  23. ncbi request reprint Rhizosecretion of stele-synthesized glucosinolates and their catabolites requires GTR-mediated import in Arabidopsis
    Deyang Xu
    DynaMo Center, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Thorvaldsensvej 40, 1871 Frederiksberg C, Denmark
    J Exp Bot . 2016
    ..The study suggests a previously undiscovered role of the import process in the rhizosecretion of root-synthesized phytochemicals...
  24. pmc Modulation of CYP79 genes and glucosinolate profiles in Arabidopsis by defense signaling pathways
    Michael Dalgaard Mikkelsen
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, opgang 10, St, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 131:298-308. 2003
    ..Thus, different defense pathways activate subsets of biosynthetic enzymes, leading to the accumulation of specific glucosinolates...
  25. pmc Metabolic engineering of valine- and isoleucine-derived glucosinolates in Arabidopsis expressing CYP79D2 from Cassava
    Michael Dalgaard Mikkelsen
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Plant Physiol 131:773-9. 2003
    ....
  26. pmc CYP83A1 and CYP83B1, two nonredundant cytochrome P450 enzymes metabolizing oximes in the biosynthesis of glucosinolates in Arabidopsis
    Peter Naur
    Plant Biochemistry Laboratory, Department of Chemistry, Center for Molecular Plant Physiology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Denmark
    Plant Physiol 133:63-72. 2003
    ....
  27. ncbi request reprint Arabidopsis mutants in the C-S lyase of glucosinolate biosynthesis establish a critical role for indole-3-acetaldoxime in auxin homeostasis
    Michael Dalgaard Mikkelsen
    Plant Biochemistry Laboratory, Department of Plant Biology, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK 1871 Frederiksberg C, Copenhagen, Denmark
    Plant J 37:770-7. 2004
    ..Our findings provide important insight to the critical role IAOx plays in auxin homeostasis as a key branching point between primary and secondary metabolism, and define a framework for further dissection of auxin biosynthesis...
  28. pmc A systems biology approach identifies a R2R3 MYB gene subfamily with distinct and overlapping functions in regulation of aliphatic glucosinolates
    Ida Elken Sønderby
    Plant Biochemistry Laboratory, Department of Plant Biology, Center for Molecular Plant Physiology PlaCe, Faculty of Life Sciences, University of Copenhagen, Copenhagen, Denmark
    PLoS ONE 2:e1322. 2007
    ..Knowledge about the genes controlling glucosinolate regulation is limited. Here, we identify three R2R3 MYB transcription factors regulating aliphatic glucosinolate biosynthesis in Arabidopsis by combining several systems biology tools...
  29. ncbi request reprint Composition and content of glucosinolates in developing Arabidopsis thaliana
    Bent Larsen Petersen
    Plant Biochemistry Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Copenhagen, Denmark
    Planta 214:562-71. 2002
    ..This indicates that there is a continuous turnover of glucosinolates during development and not only upon tissue disruption...
  30. pmc Functional analysis of the tandem-duplicated P450 genes SPS/BUS/CYP79F1 and CYP79F2 in glucosinolate biosynthesis and plant development by Ds transposition-generated double mutants
    Titima Tantikanjana
    Department of Plant Biology and Agronomy, University of California, Davis, California 95616, USA
    Plant Physiol 135:840-8. 2004
    ..This study also provides further demonstration of how gluconisolate biosynthesis, regarded as secondary metabolism, is intricately linked with hormone homeostatis and hence with plant growth and development...
  31. ncbi request reprint Altering glucosinolate profiles modulates disease resistance in plants
    Günter Brader
    Division of Genetics, Department of Biological and Environmental Sciences, Faculty of Biosciences, Viikki Biocenter, University of Helsinki, PO Box 56, FIN 00014 Helsinki, Finland
    Plant J 46:758-67. 2006
    ..Arabidopsis with modified GS profiles provide important tools for evaluating the biological effects of individual GSs and thereby show potential as biotechnological tools for the generation of plants with tailor-made disease resistance...
  32. pmc Linking metabolic QTLs with network and cis-eQTLs controlling biosynthetic pathways
    Adam M Wentzell
    Department of Plant Sciences, University of California Davis, Davis, California, USA
    PLoS Genet 3:1687-701. 2007
    ..This suggests that natural variation in transcripts may significantly impact phenotypic variation, but that natural variation in metabolites or their enzymatic loci can feed back to affect the transcripts...