Karel Kleparnik

Summary

Affiliation: Institute of Analytical Chemistry
Country: Czech Republic

Publications

  1. doi request reprint Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Veveri 97, 602 00, Brno, Czech Republic
    Methods Mol Biol 1199:33-54. 2014
  2. doi request reprint Recent advances in the development of single cell analysis--a review
    Karel Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic Electronic address
    Anal Chim Acta 800:12-21. 2013
  3. ncbi request reprint DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide
    K Kleparnik
    Institute of Analytical Chemistry, Brno, The Czech Republic
    Electrophoresis 19:695-700. 1998
  4. doi request reprint Recent advances in the combination of capillary electrophoresis with mass spectrometry: from element to single-cell analysis
    Karel Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 34:70-85. 2013
  5. doi request reprint Capillary electrophoresis immunoassays with conjugated quantum dots
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 32:1217-23. 2011
  6. doi request reprint Analyte transport in liquid junction nano-electrospray interface between capillary electrophoresis and mass spectrometry
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 31:879-85. 2010
  7. ncbi request reprint Unsteady transport phenomena in free-flow electrophoresis--prerequisite of ultrafast sample cleaning in microfluidic devices
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 25:3633-42. 2004
  8. ncbi request reprint Fast separation of DNA sequencing fragments in highly alkaline solutions of linear polyacrylamide using electrophoresis in bare silica capillaries
    K Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of Czech Republic, Brno
    Electrophoresis 22:783-8. 2001
  9. ncbi request reprint Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries
    K Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno
    Electrophoresis 21:238-46. 2000
  10. ncbi request reprint Detection of DNA fragmentation in a single apoptotic cardiomyocyte by electrophoresis on a microfluidic device
    Karel Kleparnik
    Institute of Analytical Chemistry Academy of Sciences of the Czech Republic
    Electrophoresis 24:3778-83. 2003

Collaborators

Detail Information

Publications26

  1. doi request reprint Analysis of quantum dots and their conjugates by capillary electrophoresis with detection of laser-induced luminescence
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Veveri 97, 602 00, Brno, Czech Republic
    Methods Mol Biol 1199:33-54. 2014
    ..The potential of capillary electrophoresis for fast analyses of nanoparticles, their conjugates with antibodies, and immunocomplexes with targeted antigens is demonstrated on examples. ..
  2. doi request reprint Recent advances in the development of single cell analysis--a review
    Karel Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic Electronic address
    Anal Chim Acta 800:12-21. 2013
    ....
  3. ncbi request reprint DNA cycle sequencing of a common restriction fragment of Staphylococcus aureus bacteriophages by capillary electrophoresis using replaceable linear polyacrylamide
    K Kleparnik
    Institute of Analytical Chemistry, Brno, The Czech Republic
    Electrophoresis 19:695-700. 1998
    ....
  4. doi request reprint Recent advances in the combination of capillary electrophoresis with mass spectrometry: from element to single-cell analysis
    Karel Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 34:70-85. 2013
    ..The combinations of MS with capillary versions of electrochromatography, ITP, IEF, and micellar electrokinetic chromatography are not included...
  5. doi request reprint Capillary electrophoresis immunoassays with conjugated quantum dots
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 32:1217-23. 2011
    ..6-43 ns were determined. The 0.1 M solution of TRIS/TAPS (pH 8.3) was found to be the optimum buffer for the separation of the antiovalbumin-ovalbumin immunocomplex from the free conjugates of QDs...
  6. doi request reprint Analyte transport in liquid junction nano-electrospray interface between capillary electrophoresis and mass spectrometry
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 31:879-85. 2010
    ..Several fold improvement in sensitivity and efficiency of the method can be expected when working under the optimum conditions...
  7. ncbi request reprint Unsteady transport phenomena in free-flow electrophoresis--prerequisite of ultrafast sample cleaning in microfluidic devices
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 25:3633-42. 2004
    ..Thus, a fast and efficient sample cleaning before subsequent analysis by electrospray ionization-mass spectrometry (ESI-MS) or another separation method can be performed...
  8. ncbi request reprint Fast separation of DNA sequencing fragments in highly alkaline solutions of linear polyacrylamide using electrophoresis in bare silica capillaries
    K Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of Czech Republic, Brno
    Electrophoresis 22:783-8. 2001
    ..A special sample introduction by diffusion is successfully applied. The experimental results demonstrate a potential of the alkaline electrolytes for an implementation in diagnostic sequencing practice...
  9. ncbi request reprint Ultrafast detection of microsatellite repeat polymorphism in endothelin 1 gene by electrophoresis in short capillaries
    K Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno
    Electrophoresis 21:238-46. 2000
    ..The developed methodology and instrumentation is advantageous for an implementation in clinical diagnostics and genetic population screening where fast analytical instrumentation amenable to automation is of paramount importance...
  10. ncbi request reprint Detection of DNA fragmentation in a single apoptotic cardiomyocyte by electrophoresis on a microfluidic device
    Karel Kleparnik
    Institute of Analytical Chemistry Academy of Sciences of the Czech Republic
    Electrophoresis 24:3778-83. 2003
    ..Therefore, the monitoring of the extent of apoptotic DNA damage of cardiac myocytes represents critical step toward understanding of the development of chronic doxorubicin-induced cardiomyopathy...
  11. ncbi request reprint Detection of the major mutation M467T causing cystinuria by single-strand conformation polymorphism analysis using capillary electrophoresis
    Karel Kleparnik
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 25:57-64. 2004
    ..0 of MFOLD software. The number of nucleotides involved in the duplex regions of theoretical structures correlates well with their real migration order in the sieving medium...
  12. ncbi request reprint A continuous-flow instrument for the determination of linear polyacrylamide stability
    Karel Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 25:2139-43. 2004
    ..The experimental results show a rapid onset of hydrolysis at a temperature of 70 degrees C. While the degree of 1 h hydrolysis was evaluated to reach about 16% at 70 degrees C and pH 13, it drops to 5% at 50 degrees C and pH 12.5...
  13. ncbi request reprint Optimization of a pressurized liquid junction nanoelectrospray interface between CE and MS for reliable proteomic analysis
    Petr Kusý
    Department of Bioanalytical Instrumentation, Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Brno, Czech Republic
    Electrophoresis 28:1964-9. 2007
    ..This arrangement allowed independent monitoring of the effects of pressure, CE voltage and geometry of the liquid junction on the spreading and dilution of the separated zones after passage through the interface...
  14. ncbi request reprint Capillary electrophoresis mass spectrometry coupling with immobilized enzyme electrospray capillaries
    Jana Krenkova
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, 602 00 Brno, Czech Republic
    J Chromatogr A 1159:110-8. 2007
    ..The optimized reactors were tested as the nanospray needles for fast identification of proteins using CE-ESI/TOF-MS...
  15. doi request reprint Simultaneous analysis of cocaine and its metabolites in urine by capillary electrophoresis-electrospray mass spectrometry using a pressurized liquid junction nanoflow interface
    Věra Hezinová
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 33:653-60. 2012
    ..15% for peak areas. Finally, urine samples, spiked with the standard mixture, were extracted using a solid-phase extraction procedure and injected under FASI conditions, providing recoveries from 80% to 94% for all analytes...
  16. doi request reprint Bioluminescence determination of active caspase-3 in single apoptotic cells
    Marcela Lišková
    Institute of Analytical Chemistry, v v i, Czech Academy of Science, Brno, Czech Republic
    Electrophoresis 34:1772-7. 2013
    ..Thus, in our best miniaturized system the mean amount as low as about 6.5 fg corresponding to 122 000 molecules of caspase-3 can be detected in individual apoptotic mouse leg cells...
  17. ncbi request reprint Electroosmotic flow in capillary channels filled with nonconstant viscosity electrolytes: exact solution of the Navier-Stokes equation
    Marek Otevrel
    Institute of Analytical Chemistry, Czech Academy of Sciences, Veveri 97, CZ 61142 Brno, Czech Republic
    Electrophoresis 23:3574-82. 2002
    ..A viscous layer at the wall affects EOF significantly, if it is thicker than the Debye length of the electric double layer. The presented description of the EOF dynamics is applicable to any microfluidic systems...
  18. ncbi request reprint DNA diagnostics by capillary electrophoresis
    Karel Kleparnik
    Institute of Analytical Chemistry, Academy of Sciences of the Czech Republic, Veveri 97, CZ 602 00 Brno, Czech Republic
    Chem Rev 107:5279-317. 2007
  19. doi request reprint Electrophoresis today and tomorrow: Helping biologists' dreams come true
    Karel Kleparnik
    Institute of Analytical Chemistry, Brno, Czech Republic
    Bioessays 32:218-26. 2010
    ..These miniaturized devices also proved the capability of single-molecule detection...
  20. doi request reprint Determination of ζ-potential, charge, and number of organic ligands on the surface of water soluble quantum dots by capillary electrophoresis
    Ivona Voráčová
    Institute of Analytical Chemistry, Czech Academy of Sciences, Brno, Czech Republic
    Electrophoresis 36:867-74. 2015
    ..The dissociation constants of organic ligands bonded on QDs surfaces estimated from the dependence of QDs charge on pH of the separation buffer were 7.8 and 7.9 for 3-mercaptopropionic acid and 6.9 for thioglycolic acid. ..
  21. ncbi request reprint Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
    Vojtěch Ledvina
    Institute of Analytical Chemistry, v v i, Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic
    Anal Bioanal Chem . 2016
    ..46/2.92 for non-apoptotic cells. Application potential of this technology in laboratory diagnostics and related medical research is discussed. Graphical abstract Miniaturized device for simultaneous analyses of individual cells...
  22. doi request reprint Self-aligning subatmospheric hybrid liquid junction electrospray interface for capillary electrophoresis
    Jana Krenkova
    Institute of Analytical Chemistry, CAS, v v i, Brno, Czech Republic
    Electrophoresis 37:414-7. 2016
    ..The liquid junction is connected to a subatmospheric electrospray chamber inducing the flow inside the ESI needle and helping the ion transport via aerodynamic focusing. ..
  23. doi request reprint Caspases and osteogenic markers--in vitro screening of inhibition impact
    Eva Adamová
    Institute of Animal Physiology and Genetics CAS, v v i, Brno, Czech Republic
    In Vitro Cell Dev Biol Anim 52:144-8. 2016
    ..The list suggests molecular interactions of caspases and osteogenic molecules and creates a background for further temporospatial and functional studies. ..
  24. doi request reprint A miniaturized device for bioluminescence analysis of caspase-3/7 activity in a single apoptotic cell
    Eva Adamová
    Institute of Analytical Chemistry, v v i, Czech Academy of Sciences, Veveri 97, 602 00, Brno, Czech Republic
    Anal Bioanal Chem 406:5389-94. 2014
    ..20 and limit of quantification as 0.65 of the amount found in a single apoptotic human stem cell. Such a sensitive method could have a wide application potential in laboratory medicine and related clinically oriented research. ..
  25. doi request reprint Photodeposited silver nanoparticles for on-column surface-enhanced Raman spectrometry detection in capillary electrophoresis
    Jan Přikryl
    Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic, Brno, Czech Republic
    J Chromatogr A 1226:43-7. 2012
    ..8 nm is demonstrated by the 3D electropherograms of rhodamines 123 and B as model samples. The limits of detection of about 49 and 150 fmol (1 and 2 μM) have been reached for rhodamine B and 123, respectively...
  26. ncbi request reprint Ionic boundaries in biological capillary electrophoresis
    Frantisek Foret
    Institute of Analytical Chemistry, Veveri 97, 61142 Brno, Czech Republic
    J Chromatogr A 1053:43-57. 2004
    ..This critical article attempts to review some of the important effects of the ionic boundaries observed or utilized during the CE of biologically important samples...