Jiri Homola

Summary

Affiliation: Institute of Radio Engineering and Electronics
Country: Czech Republic

Publications

  1. ncbi request reprint Present and future of surface plasmon resonance biosensors
    Jiri Homola
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Anal Bioanal Chem 377:528-39. 2003
  2. ncbi request reprint Multi-analyte surface plasmon resonance biosensing
    Jiri Homola
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 18251 Prague, Czech Republic
    Methods 37:26-36. 2005
  3. ncbi request reprint A new surface plasmon resonance sensor for high-throughput screening applications
    Marek Piliarik
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 18251 Prague, Czech Republic
    Biosens Bioelectron 20:2104-10. 2005
  4. doi request reprint High-resolution biosensor based on localized surface plasmons
    Marek Piliarik
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Opt Express 20:672-80. 2012
  5. ncbi request reprint Copolymer Brush-Based Ultralow-Fouling Biorecognition Surface Platform for Food Safety
    Hana Vaisocherová-Lísalová
    Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberska 57, Prague, Czech Republic
    Anal Chem 88:10533-10539. 2016
  6. doi request reprint Surface plasmon resonance sensing of nucleic acids: a review
    Hana Sípová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Anal Chim Acta 773:9-23. 2013
  7. doi request reprint Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma
    Tomáš Springer
    Institute of Photonics and Electronics AS CR, v v i, Chaberska 57, 182 51 Prague, Czech Republic
    Anal Bioanal Chem 404:2869-75. 2012
  8. doi request reprint Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
    Hana Sípová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská, Prague, Czech Republic
    Anal Bioanal Chem 399:2343-50. 2011
  9. doi request reprint High-throughput SPR sensor for food safety
    Marek Piliarik
    Institute of Photonics and Electronics, Chaberska 57, 182 51 Prague, Czech Republic
    Biosens Bioelectron 24:1399-404. 2009
  10. doi request reprint Surface plasmon resonance biosensing
    Marek Piliarik
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Methods Mol Biol 503:65-88. 2009

Collaborators

  • Hana Vaisocherová
  • Pavel Adam
  • Milan Houska
  • Xue Song
  • Michaela Dostalova Merkerova
  • Jan Mrazek
  • Ivan Barvik
  • Kai Wang
  • Cesar Rodriguez-Emmenegger
  • Jaroslav Cermak
  • Tomas Zima
  • Bohdan Schneider
  • Hana Sípová
  • Tomáš Springer
  • Marek Piliarik
  • N Scott Lynn
  • Markéta Bocková
  • Hana Vaisocherová-Lísalová
  • Pavel Kvasnička
  • Maria Laura Ermini
  • Milan Vala
  • Josef Stepanek
  • Barbora Spacková
  • Ivana Víšová
  • Petr Šedivák
  • Kristýna Pimková
  • Joachim R Krenn
  • Nicolle Galler
  • Aleksandra Friedl
  • Radan Slavík
  • Katerina Levová
  • Josefína Lamačová
  • Ognen Pop-Georgievski
  • Eduard Brynda
  • František Surman
  • Xue Chadtová Song
  • Marta Kalousova
  • Erika Gedeonová
  • Tomas Riedel
  • Jaroslav Nunvar
  • Iva Nečasová
  • Radek Liboska
  • Sarka Rosenbergová
  • Jani Jabloňků
  • Ivan Rosenberg
  • Victor Coello
  • José Israel Martínez-López
  • Magdalena Petrová
  • Ondrej Pav
  • Héctor R Siller
  • Dominik Rejman
  • Ondřej Šimák
  • Pavel Novak
  • Karel Chadt
  • Jan E Dyr
  • David Vrba
  • Katerina Hegnerová
  • Jiri Suttnar
  • Sergey Leshkov
  • David Galas
  • AIMEE M DUDLEY
  • Shile Zhang
  • Ivo Tichý
  • Lucie Párová

Detail Information

Publications39

  1. ncbi request reprint Present and future of surface plasmon resonance biosensors
    Jiri Homola
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Anal Bioanal Chem 377:528-39. 2003
    ..This paper reviews fundamentals of SPR affinity biosensors and discusses recent advances in development and applications of SPR biosensors...
  2. ncbi request reprint Multi-analyte surface plasmon resonance biosensing
    Jiri Homola
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 18251 Prague, Czech Republic
    Methods 37:26-36. 2005
    ..In addition, we report application of a recently developed multichannel SPR sensor based on spectroscopy of surface plasmons and wavelength division multiplexing of sensing channels to multi-analyte detection...
  3. ncbi request reprint A new surface plasmon resonance sensor for high-throughput screening applications
    Marek Piliarik
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 18251 Prague, Czech Republic
    Biosens Bioelectron 20:2104-10. 2005
    ..Experimental data on detection of human choriogonadotropin (hCG) suggest that in conjunction with monoclonal antibodies against hCG, the reported SPR imaging sensor is capable of detecting hCG at concentrations lower than 500 ng/ml...
  4. doi request reprint High-resolution biosensor based on localized surface plasmons
    Marek Piliarik
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Opt Express 20:672-80. 2012
    ..We demonstrate a limit of detection of 100 pM and a surface density resolution of only 35 fg×mm-2 that corresponds to less than one DNA molecule per nanoparticle on average...
  5. ncbi request reprint Copolymer Brush-Based Ultralow-Fouling Biorecognition Surface Platform for Food Safety
    Hana Vaisocherová-Lísalová
    Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberska 57, Prague, Czech Republic
    Anal Chem 88:10533-10539. 2016
    ....
  6. doi request reprint Surface plasmon resonance sensing of nucleic acids: a review
    Hana Sípová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Anal Chim Acta 773:9-23. 2013
    ....
  7. doi request reprint Biofunctionalized gold nanoparticles for SPR-biosensor-based detection of CEA in blood plasma
    Tomáš Springer
    Institute of Photonics and Electronics AS CR, v v i, Chaberska 57, 182 51 Prague, Czech Republic
    Anal Bioanal Chem 404:2869-75. 2012
    ..Moreover, the limit of detection achieved using this approach is better by a factor of more than 1,000 than limits of detection reported so far for CEA in blood plasma using SPR biosensors...
  8. doi request reprint Streptavidin-enhanced assay for sensitive and specific detection of single nucleotide polymorphism in TP53
    Hana Sípová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská, Prague, Czech Republic
    Anal Bioanal Chem 399:2343-50. 2011
    ..The assay is applied to detection of a short synthetic analogue of TP53 containing a "hot spot"-single nucleotide mismatch frequently mutated in germ line cancer-at levels down to 40 pM...
  9. doi request reprint High-throughput SPR sensor for food safety
    Marek Piliarik
    Institute of Photonics and Electronics, Chaberska 57, 182 51 Prague, Czech Republic
    Biosens Bioelectron 24:1399-404. 2009
    ..Detection of specific DNA or RNA sequences can be performed in less than 15 min by the reported SPR sensor...
  10. doi request reprint Surface plasmon resonance biosensing
    Marek Piliarik
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Methods Mol Biol 503:65-88. 2009
    ..This chapter reviews the main principles of SPR biosensor technology and discusses applications of this technology for rapid, sensitive and specific detection of chemical and biological analytes...
  11. pmc Shielding effect of monovalent and divalent cations on solid-phase DNA hybridization: surface plasmon resonance biosensor study
    Tomáš Springer
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 182 51 Prague, Czech Republic
    Nucleic Acids Res 38:7343-51. 2010
    ..Moreover, it is demonstrated that the use of a buffer with the appropriate cation composition can improve the discrimination of complementary and point mismatched DNA targets...
  12. doi request reprint Compact and low-cost biosensor based on novel approach to spectroscopy of surface plasmons
    Marek Piliarik
    Institute of Photonics and Electronics ASCR, Chaberska 57, CZ 18251 Prague, Czech Republic
    Biosens Bioelectron 24:3430-5. 2009
    ..It is demonstrated that the SPRCD sensor is able to measure bulk refractive index changes as small as 3 x 10(-7) RIU (refractive index units) and to detect short oligonucleotides in concentrations down to 200 pM...
  13. doi request reprint Real-time monitoring of biomolecular interactions in blood plasma using a surface plasmon resonance biosensor
    Tomáš Springer
    Department of Optical Sensors, Institute of Photonics and Electronics AS CR, v v i, Chaberska 57, 182 51 Prague, Czech Republic
    Anal Bioanal Chem 398:1955-61. 2010
    ..In the model experiment, this approach is shown to enable direct detection of hCG in blood plasma at levels which are five times lower than those detected using the conventional detection approach...
  14. pmc 5'-O-Methylphosphonate nucleic acids--new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes
    Hana Sípová
    Institute of Photonics and Electronics AS CR, v v i, Chaberska 57, 182 51 Prague, Czech Republic, Institute of Organic Chemistry and Biochemistry AS CR, v v i, Flemingovo nam 2, 166 10 Prague, Czech Republic and Faculty of Mathematics and Physics, Charles University in Prague, Ke Karlovu 3, 121 16 Prague, Czech Republic
    Nucleic Acids Res 42:5378-89. 2014
    ..We propose for this type of AOs, working via the RNase H mechanism, the abbreviation MEPNA (MEthylPhosphonate Nucleic Acid). ..
  15. doi request reprint Toward single-molecule detection with sensors based on propagating surface plasmons
    Pavel Kvasnička
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Opt Lett 37:163-5. 2012
    ..A protein monolayer has been shown to produce a response of 5000 times the baseline noise, suggesting that as few as ~500 proteins could be detected by the sensor...
  16. doi request reprint Theoretical analysis of a fiber optic surface plasmon resonance sensor utilizing a Bragg grating
    Barbora Spacková
    Institute of Photonics and Electronics ASCR v v i, Chaberska 57, Prague, Czech Republic
    Opt Express 17:23254-64. 2009
    ..The sensitivity of these dips to changes in the refractive index of the analyte is calculated. Moreover, the refractive index resolution of the sensor was estimated to be better than 2 x 10(-6) RIU...
  17. doi request reprint Surface plasmon resonance biosensor for parallelized detection of protein biomarkers in diluted blood plasma
    Marek Piliarik
    Institute of Photonics and Electronics AS CR v v i, Chaberska 57, CZ 18251 Prague 8, Czech Republic
    Biosens Bioelectron 26:1656-61. 2010
    ..Limits of detection as low as 45 ng/mL (ALCAM) and 100 ng/mL (hCG) were achieved in blood plasma samples...
  18. pmc Surface plasmon resonance biosensor for rapid label-free detection of microribonucleic acid at subfemtomole level
    Hana Sípová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberská 57, 18251 Prague, Czech Republic
    Anal Chem 82:10110-5. 2010
    ..The methodology is evaluated for analysis of miRNA from mouse liver tissues and is found to yield results which agree well with those provided by the quantitative polymerase chain reaction (qPCR)...
  19. ncbi request reprint Fabrication of nanoplasmonic arrays with square symmetry using spin-coating method
    Aleksandra Friedl
    Institute of Photonics and Electronics, Chaberska 57, 182 51 Prague, Czech Republic
    J Nanosci Nanotechnol 11:2528-32. 2011
    ..A few hundred square micrometers large, continuous and fully defect-free areas of gold nanoparticles were produced on the nearly entire surface of the templated substrates...
  20. ncbi request reprint Surface plasmon resonance biosensor for direct detection of antibody against Epstein-Barr virus
    Hana Vaisocherová
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 182 51 Prague, Czech Republic
    Biosens Bioelectron 22:1020-6. 2007
    ..It was demonstrated that the sensor is capable of detecting concentrations of anti-EBNA as low as 0.2 ng/ml (approximately 1 pM) both in buffer and 1% human serum and can be stored and regenerated for repeated use...
  21. doi request reprint Rapid and sensitive detection of multiple microRNAs in cell lysate by low-fouling surface plasmon resonance biosensor
    Hana Vaisocherová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 18251 Prague, Czech Republic
    Biosens Bioelectron 70:226-31. 2015
    ..The results revealed significantly higher levels of miR-16 in all the clinical EL samples compared to the other measured miRNAs...
  22. doi request reprint Functionalized ultra-low fouling carboxy- and hydroxy-functional surface platforms: functionalization capacity, biorecognition capability and resistance to fouling from undiluted biological media
    Hana Vaisocherová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, v v i, Chaberska 57, 18251 Prague, Czech Republic
    Biosens Bioelectron 51:150-7. 2014
    ..Effect of analyte size on biorecognition activity of functionalized coatings was investigated and it was shown that the best performance in terms of overall fouling resistance and biorecognition capability is provided by pCBAA...
  23. doi request reprint Surface plasmon resonance sensors for detection of chemical and biological species
    Jiri Homola
    Institute of Photonics and Electronics ASCR, Chaberska 57, 182 51 Prague 8, Czech Republic
    Chem Rev 108:462-93. 2008
  24. doi request reprint Surface plasmon resonance biosensor for the detection of VEGFR-1--a protein marker of myelodysplastic syndromes
    Kristýna Pimková
    Institute of Hematology and Blood Transfusion, Prague, Czech Republic
    Anal Bioanal Chem 402:381-7. 2012
    ..The detection limit of 25 ng/mL is achieved. In addition, we demonstrate that the functional surface of the sensor can be regenerated for repeated use...
  25. doi request reprint Analytical value of detecting an individual molecular binding event: the case of the surface plasmon resonance biosensor
    Hana Sípová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 182 51 Prague, Czech Republic
    Anal Chem 84:30-3. 2012
    ....
  26. doi request reprint A dual surface plasmon resonance assay for the determination of ribonuclease H activity
    Hana Sípová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 182 51 Prague, Czech Republic
    Biosens Bioelectron 26:1605-11. 2010
    ..The potential of the assay is demonstrated in the study in which the ability of natural and modified oligonucleotides to activate RNase H is examined...
  27. doi request reprint Surface plasmon resonance study on HIV-1 integrase strand transfer activity
    Hana Vaisocherová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 182 51, Prague, Czech Republic
    Anal Bioanal Chem 393:1165-72. 2009
    ....
  28. doi request reprint Biosensor Enhancement Using Grooved Micromixers: Part II, Experimental Studies
    N Scott Lynn
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Anal Chem 87:5524-30. 2015
    ..For realistic experimental conditions, the enhancement that the SHM can provide is in the range of 1 < Emix < 5 (0% < improvement < 400%). ..
  29. doi request reprint Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples
    Hana Vaisocherová-Lísalová
    Institute of Photonics and Electronics, Czech Academy of Sciences, Chaberska 57, Prague, Czech Republic
    Biosens Bioelectron 80:84-90. 2016
    ..7 × 10(3)CFU/mL for Salmonella sp., respectively. In addition, we demonstrate the simultaneous detection of E. coli and Salmonella sp. in hamburger sample using a multichannel SPR biosensor having appropriate functional coatings...
  30. doi request reprint Biosensing enhancement using passive mixing structures for microarray-based sensors
    N Scott Lynn
    Institute of Photonics and Electronics, Chaberska 57, 18251 Prague, Czech Republic
    Biosens Bioelectron 54:506-14. 2014
    ..The results of this study can be applied to any experimental system using microfluidic sample delivery methods for microarray detection techniques...
  31. doi request reprint Enhancement of affinity-based biosensors: effect of sensing chamber geometry on sensitivity
    N Scott Lynn
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Lab Chip 13:1413-21. 2013
    ..We utilize these results to discuss the applicability of this scaling behavior with respect to a generalized affinity-based biosensor...
  32. doi request reprint Monitoring RAYT activity by surface plasmon resonance biosensor
    Markéta Bocková
    Institute of Photonics and Electronics AS CR, v v i, Chaberska 57, 182 51, Prague, Czech Republic
    Anal Bioanal Chem 407:3985-93. 2015
    ..The characteristics of the RAYT-DNA interaction obtained by means of the SPR approach were compared with the results of SDS-PAGE analysis. ..
  33. doi request reprint Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications
    Hana Vaisocherová
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague 8, 18251, Czech Republic
    Anal Bioanal Chem 407:3927-53. 2015
    ..Special attention is given to biofunctional coatings for label-free bioanalysis of blood plasma and serum for medical diagnostics. ..
  34. doi request reprint Enhancing sensitivity of surface plasmon resonance biosensors by functionalized gold nanoparticles: size matters
    Tomáš Springer
    Institute of Photonics and Electronics AS CR, v v i, Chaberska 57, 182 51, Prague, Czech Republic
    Anal Chem 86:10350-6. 2014
    ..Our results indicate that, while Sσ increases with the size of the N-AuNP, the ability of the functionalized surface of the sensor to bind the N-AuNPs is affected by steric effects and decreases with the size of N-AuNP. ..
  35. ncbi request reprint Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method
    Hana Vaisocherová
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, 182 51 Prague, Czech Republic
    Biopolymers 82:394-8. 2006
    ..It was demonstrated that the sensor is capable of detecting complementary 23-mer ONs in concentrations as low as 0.1 nM with high specificity and reproducibility...
  36. doi request reprint Surface plasmon resonance biosensor for detection of pregnancy associated plasma protein A2 in clinical samples
    Markéta Bocková
    Department of Optical Sensors, Institute of Photonics and Electronics of the CAS, Chaberska 57, 18251, Prague 8, Czech Republic
    Anal Bioanal Chem 408:7265-9. 2016
    ..Graphical Abstract Temporal sensor response corresponding to respective steps of the assay for detection of PAPP-A2 in buffer. ..
  37. doi request reprint Biosensor enhancement using grooved micromixers: part I, numerical studies
    N Scott Lynn
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Anal Chem 87:5516-23. 2015
    ..The results presented herein are applicable to a very wide range of biosensor transduction mechanisms and target analytes. ..
  38. ncbi request reprint Optical multilayers for LED-based surface plasmon resonance sensors
    Radan Slavík
    Institute of Radio Engineering and Electronics, Academy of Sciences of the Czech Republic, Prague
    Appl Opt 45:3752-9. 2006
    ..We show that the sensor can be designed to have similar sensitivity and even better resolution compared with those for a conventional configuration...
  39. doi request reprint Local refractive index sensitivity of plasmonic nanoparticles
    Marek Piliarik
    Institute of Photonics and Electronics, Academy of Sciences of the Czech Republic, Chaberska 57, Prague, Czech Republic
    Opt Express 19:9213-20. 2011
    ..The results indicate that the sensitivity is strongly correlated with the profile of the LSP electric field...