Eva Sykova

Summary

Affiliation: Academy of Sciences of the Czech Republic
Country: Czech Republic

Publications

  1. ncbi Autologous bone marrow transplantation in patients with subacute and chronic spinal cord injury
    Eva Sykova
    Center for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic
    Cell Transplant 15:675-87. 2006
  2. ncbi Diffusion constraints and neuron-glia interaction during aging
    E Sykova
    Department of Neuroscience, Second Medical Faculty, Charles University, Prague, Czech Republic
    Exp Gerontol 33:837-51. 1998
  3. doi Magnetic resonance imaging of stem cell migration
    Eva Sykova
    Institute of Experimental Medicine ASCR, Prague, Czech Republic
    Methods Mol Biol 750:79-90. 2011
  4. ncbi In vivo tracking of stem cells in brain and spinal cord injury
    Eva Sykova
    Institute of Experimental Medicine ASCR, EU Centre of Excellence, Prague, Czech Republic
    Prog Brain Res 161:367-83. 2007
  5. ncbi Extrasynaptic transmission and the diffusion parameters of the extracellular space
    Eva Sykova
    Department of Neuroscience, Charles University, 2nd Medical Faculty, Prague, Czech Republic
    Neurochem Int 52:5-13. 2008
  6. ncbi Migration, fate and in vivo imaging of adult stem cells in the CNS
    E Sykova
    Department of Neuroscience, Institute of Experimental Medicine ASCR, Prague, Czech Republic
    Cell Death Differ 14:1336-42. 2007
  7. ncbi Magnetic resonance tracking of transplanted stem cells in rat brain and spinal cord
    Eva Sykova
    Institute of Experimental Medicine ASCR, Center for Cell Therapy and Tissue Repair and Department of Neuroscience, Second Medical Faculty, Charles University, Prague, Czech Republic
    Neurodegener Dis 3:62-7. 2006
  8. ncbi Bone marrow stem cells and polymer hydrogels--two strategies for spinal cord injury repair
    Eva Sykova
    Institute of Experimental Medicine ASCR, Videnska, 1083 142 20, Prague 4, Czech Republic
    Cell Mol Neurobiol 26:1113-29. 2006
  9. ncbi Reduced extracellular space in the brain of tenascin-R- and HNK-1-sulphotransferase deficient mice
    Eva Sykova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Eur J Neurosci 22:1873-80. 2005
  10. ncbi Magnetic resonance tracking of implanted adult and embryonic stem cells in injured brain and spinal cord
    Eva Sykova
    Institute of Experimental Medicine ASCR, Vídeská 1083, 140 20 Prague 4, Czech Republic
    Ann N Y Acad Sci 1049:146-60. 2005

Collaborators

Detail Information

Publications83

  1. ncbi Autologous bone marrow transplantation in patients with subacute and chronic spinal cord injury
    Eva Sykova
    Center for Cell Therapy and Tissue Repair, Charles University, Prague, Czech Republic
    Cell Transplant 15:675-87. 2006
    ..Trials involving a larger population of patients and different cell types are needed before further conclusions can be drawn...
  2. ncbi Diffusion constraints and neuron-glia interaction during aging
    E Sykova
    Department of Neuroscience, Second Medical Faculty, Charles University, Prague, Czech Republic
    Exp Gerontol 33:837-51. 1998
    ..We can, therefore, assume that the observed changes in ECS diffusion parameters during aging can contribute to functional deficits and memory loss...
  3. doi Magnetic resonance imaging of stem cell migration
    Eva Sykova
    Institute of Experimental Medicine ASCR, Prague, Czech Republic
    Methods Mol Biol 750:79-90. 2011
    ....
  4. ncbi In vivo tracking of stem cells in brain and spinal cord injury
    Eva Sykova
    Institute of Experimental Medicine ASCR, EU Centre of Excellence, Prague, Czech Republic
    Prog Brain Res 161:367-83. 2007
    ..Our studies demonstrate that magnetic resonance imaging (MRI) of grafted adult as well as ESCs labeled with iron oxide nanoparticles is a useful method for evaluating cellular migration toward a lesion site...
  5. ncbi Extrasynaptic transmission and the diffusion parameters of the extracellular space
    Eva Sykova
    Department of Neuroscience, Charles University, 2nd Medical Faculty, Prague, Czech Republic
    Neurochem Int 52:5-13. 2008
    ..The various changes occurring during pathological states can be important for diagnosis, drug delivery and treatment...
  6. ncbi Migration, fate and in vivo imaging of adult stem cells in the CNS
    E Sykova
    Department of Neuroscience, Institute of Experimental Medicine ASCR, Prague, Czech Republic
    Cell Death Differ 14:1336-42. 2007
    ..This review will focus on cell labeling for MRI and the use of MSCs in experimental and clinical studies for the treatment of brain and spinal cord injuries...
  7. ncbi Magnetic resonance tracking of transplanted stem cells in rat brain and spinal cord
    Eva Sykova
    Institute of Experimental Medicine ASCR, Center for Cell Therapy and Tissue Repair and Department of Neuroscience, Second Medical Faculty, Charles University, Prague, Czech Republic
    Neurodegener Dis 3:62-7. 2006
    ..Magnetic resonance imaging provides a noninvasive method to study the fate of transplanted cells in vivo...
  8. ncbi Bone marrow stem cells and polymer hydrogels--two strategies for spinal cord injury repair
    Eva Sykova
    Institute of Experimental Medicine ASCR, Videnska, 1083 142 20, Prague 4, Czech Republic
    Cell Mol Neurobiol 26:1113-29. 2006
    ....
  9. ncbi Reduced extracellular space in the brain of tenascin-R- and HNK-1-sulphotransferase deficient mice
    Eva Sykova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Eur J Neurosci 22:1873-80. 2005
    ..The altered diffusion of neuroactive substances in the brain will inevitably affect extrasynaptic transmission, neuron-glia interactions and synaptic efficacy...
  10. ncbi Magnetic resonance tracking of implanted adult and embryonic stem cells in injured brain and spinal cord
    Eva Sykova
    Institute of Experimental Medicine ASCR, Vídeská 1083, 140 20 Prague 4, Czech Republic
    Ann N Y Acad Sci 1049:146-60. 2005
    ..Our studies demonstrate that grafted adult as well as embryonic stem cells labeled with iron-oxide nanoparticles migrate into a lesion site in brain as well as in spinal cord...
  11. pmc Changes in extracellular space size and geometry in APP23 transgenic mice: a model of Alzheimer's disease
    Eva Sykova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Proc Natl Acad Sci U S A 102:479-84. 2005
    ....
  12. ncbi Glia and volume transmission during physiological and pathological states
    E Sykova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, and Department of Neuroscience, Second Medical Faculty, Charles University, Prague, Czech Republic
    J Neural Transm (Vienna) 112:137-47. 2005
    ..In addition, changes occurring during pathological states can be important for diagnosis, drug delivery and treatment...
  13. ncbi Extrasynaptic volume transmission and diffusion parameters of the extracellular space
    E Sykova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague and Department of Neuroscience, Charles University, Second Medical Faculty, Videnska 1083, 14220 Prague 4, Czech Republic
    Neuroscience 129:861-76. 2004
    ..The various changes in tissue diffusivity occurring during many pathological states are important for diagnosis, drug delivery and treatment...
  14. ncbi Glial swelling and astrogliosis produce diffusion barriers in the rat spinal cord
    E Sykova
    Department of Neuroscience, 2nd Medical Faculty, Charles University, Czech Republic
    Glia 25:56-70. 1999
    ..This could lead to the impairment of the diffusion of neuroactive substances, extrasynaptic transmission, "crosstalk" between synapses and neuron-glia communication...
  15. ncbi Glial cells and volume transmission in the CNS
    E Sykova
    Department of Neuroscience, 2nd Medical Faculty, Charles University and Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague
    Neurochem Int 36:397-409. 2000
    ..In turn, ECS diffusion parameters affect neuron-glia communication, ionic homeostasis and movement and/or accumulation of neuroactive substances in the brain...
  16. ncbi Diffusion properties of the brain in health and disease
    Eva Sykova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    Neurochem Int 45:453-66. 2004
    ....
  17. ncbi Glial diffusion barriers during aging and pathological states
    E Sykova
    Department of Neuroscience, 2nd Medical Faculty, Charles University, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic
    Prog Brain Res 132:339-63. 2001
    ....
  18. ncbi The relationship between changes in intrinsic optical signals and cell swelling in rat spinal cord slices
    Eva Sykova
    Department of Neuroscience, 2nd Medical Faculty, Charles University, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Neuroimage 18:214-30. 2003
    ..Thus, light transmittance changes do not correlate with changes in ECS volume but are associated with neuronal activity and morphological changes in astrocytes...
  19. doi Adjusting the chemical and physical properties of hydrogels leads to improved stem cell survival and tissue ingrowth in spinal cord injury reconstruction: a comparative study of four methacrylate hydrogels
    Ales Hejcl
    1 Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Stem Cells Dev 22:2794-805. 2013
    ..Our results demonstrate that the physical and chemical properties of the HPMA-SP-RGD hydrogel show the best combination for bridging a spinal cord lesion, while the HEMA-MOETACl hydrogel serves as the best carrier of MSCs. ..
  20. ncbi Magnetic resonance tracking of transplanted bone marrow and embryonic stem cells labeled by iron oxide nanoparticles in rat brain and spinal cord
    Pavla Jendelova
    Institute of Experimental Medicine Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Neurosci Res 76:232-43. 2004
    ..Iron oxide nanoparticles can therefore be used as a marker for the long-term noninvasive MR tracking of implanted stem cells...
  21. doi Acute and delayed implantation of positively charged 2-hydroxyethyl methacrylate scaffolds in spinal cord injury in the rat
    Ales Hejcl
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Czech Republic
    J Neurosurg Spine 8:67-73. 2008
    ..Hydrogels are nontoxic, chemically inert synthetic polymers with a high water content and large surface area that provide mechanical support for cells and axons when implanted into spinal cord tissue...
  22. ncbi Transplantation of embryonic neuroectodermal progenitor cells into the site of a photochemical lesion: immunohistochemical and electrophysiological analysis
    Miroslava Anderova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Neurobiol 66:1084-100. 2006
    ..Further, our results suggest that embryonic neuroectodermal progenitor cells may hold considerable promise for the repair of ischemic brain lesions...
  23. doi Highly superporous cholesterol-modified poly(2-hydroxyethyl methacrylate) scaffolds for spinal cord injury repair
    Sarka Kubinova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 14220 Prague 4, Czech Republic
    J Biomed Mater Res A 99:618-29. 2011
    ..The results show that highly superporous cholesterol-modified PHEMA hydrogels have bioadhesive properties and are able to bridge a spinal cord lesion...
  24. ncbi High extracellular K(+) evokes changes in voltage-dependent K(+) and Na (+) currents and volume regulation in astrocytes
    Helena Neprasova
    Department of Neurobiology, Institute of Experimental Medicine, The Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Pflugers Arch 453:839-49. 2007
    ..We conclude that exposure to high K(+), an early event leading to astrogliosis, caused not only morphological changes in astrocytes but also changes in their membrane properties and cell volume regulation...
  25. doi The role of nitric oxide in the development of neurogenic pulmonary edema in spinal cord-injured rats: the effect of preventive interventions
    Jiri Sedy
    Institute of Experimental Medicine and Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Am J Physiol Regul Integr Comp Physiol 297:R1111-7. 2009
    ..In conclusion, nitric oxide has a partial protective effect on NPE development because it attenuates sympathetic vasoconstriction and consequent baroreflex-induced bradycardia following spinal cord injury...
  26. ncbi Treating spinal cord injury in rats with a combination of human fetal neural stem cells and hydrogels modified with serotonin
    Jiri Ruzicka
    Institute of Experimental Medicine, ASCR, Prague, Czech Republic
    Acta Neurobiol Exp (Wars) 73:102-15. 2013
    ..Further modifications will be necessary to achieve stable long term improvement in functional outcome...
  27. pmc The use of dopamine-hyaluronate associate-coated maghemite nanoparticles to label cells
    Michal Babic
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, Prague, Czech Republic
    Int J Nanomedicine 7:1461-74. 2012
    ..45 (weight/ weight) and DPA/HA = 0.038 (weight/weight) ratios. The particles were used as a contrast agent in magnetic resonance imaging for the labeling and visualization of cells...
  28. pmc Cell death/proliferation and alterations in glial morphology contribute to changes in diffusivity in the rat hippocampus after hypoxia-ischemia
    Miroslava Anderova
    Institute of Experimental Medicine, Department of Cellular Neurophysiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Cereb Blood Flow Metab 31:894-907. 2011
    ....
  29. ncbi Poly(N,N-dimethylacrylamide)-coated maghemite nanoparticles for stem cell labeling
    Michal Babic
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 16206 Prague 6, Czech Republic
    Bioconjug Chem 20:283-94. 2009
    ..In vivo MR imaging ofPDMAAm-modified iron oxide-labeled rMSCs implanted in a rat brain confirmed their better resolution compared with Endorem-labeled cells...
  30. ncbi Imaging the fate of implanted bone marrow stromal cells labeled with superparamagnetic nanoparticles
    Pavla Jendelova
    Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, and Institute of Experimental Medicine, Academy of Sciences of Czech Republic, Prague, Czech Republic
    Magn Reson Med 50:767-76. 2003
    ..Our study demonstrates that a commercially available contrast agent can be used as a marker for the long-term noninvasive MR tracking of implanted cells...
  31. doi HPMA-RGD hydrogels seeded with mesenchymal stem cells improve functional outcome in chronic spinal cord injury
    Ales Hejcl
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Stem Cells Dev 19:1535-46. 2010
    ..We conclude that 5 weeks after injury, HPMA-RGD hydrogels seeded with MSCs can successfully bridge a spinal cord cavity and provide a scaffold for tissue regeneration. This treatment leads to functional improvement even in chronic SCI...
  32. ncbi Changes in diffusion parameters, energy-related metabolites and glutamate in the rat cortex after transient hypoxia/ischemia
    Ales Homola
    Department of Neuroscience and Centre for Cell Therapy and Tissue Repair, 2nd Medical Faculty, Prague, Czech Republic
    Neurosci Lett 404:137-42. 2006
    ..The decrease in the ECS volume fraction can therefore contribute to an increased accumulation of toxic metabolites, which may aggravate functional deficits and lead to damage of the central nervous system (CNS)...
  33. doi Poly(L-lysine)-modified iron oxide nanoparticles for stem cell labeling
    Michal Babic
    Institute of Macromolecular Chemistry, v v i, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 162 06 Prague 6, Czech Republic
    Bioconjug Chem 19:740-50. 2008
    ..In comparison with Endorem, nanoparticles modified with PLL of an optimum molecular weight demonstrated a higher efficiency of intracellular uptake by MSC cells...
  34. ncbi The use of oligoperoxide-coated magnetic nanoparticles to label stem cells
    Daniela Sponarová
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq 2, 162 06 Prague 6, Czech Republic
    J Biomed Nanotechnol 7:384-94. 2011
    ..Such particles are thus promising for in vivo MR imaging of transplanted cells. Moreover, PVBM offers the possibility of additional modification by grafting compounds that reduce non-specific protein adsorption...
  35. pmc The impact of alpha-syntrophin deletion on the changes in tissue structure and extracellular diffusion associated with cell swelling under physiological and pathological conditions
    Lesia Dmytrenko
    Institute of Experimental Medicine AS CR, v v i, Prague, Czech Republic
    PLoS ONE 8:e68044. 2013
    ..The substantially altered ECS diffusion parameters will likely affect the movement of neuroactive substances and/or trophic factors, which in turn may modulate the extent of tissue damage and/or drug distribution. ..
  36. ncbi Postnatal hypobaric hypoxia in rats impairs water maze learning and the morphology of neurones and macroglia in cortex and hippocampus
    Zuzana Simonova
    Department of Neuroscience, Center for Cell Therapy and Tissue Repair, Charles University, Second Medical Faculty, 150 18 Prague 5, Czech Republic
    Behav Brain Res 141:195-205. 2003
    ..Hypobaric hypoxia delays the maturation of neurones and substantially affects macroglia in the cortex and hippocampus...
  37. ncbi Transplantation of bone marrow stem cells as well as mobilization by granulocyte-colony stimulating factor promotes recovery after spinal cord injury in rats
    Lucia Urdzíková
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Neurotrauma 23:1379-91. 2006
    ..We conclude that treatment with three different bone marrow cell populations had a positive effect on behavioral outcome and histopathological assessment after SCI, which was most pronounced after MSC injection...
  38. ncbi Brain metabolism and diffusion in the rat cerebral cortex during pilocarpine-induced status epilepticus
    Karel Slais
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Exp Neurol 209:145-54. 2008
    ....
  39. ncbi Low concentration of isoflurane promotes the development of neurogenic pulmonary edema in spinal cord injured rats
    Jiri Sedy
    Institute of Experimental Medicine, ASCR, Prague, Czech Republic
    J Neurotrauma 24:1487-501. 2007
    ..The optimal concentration of anesthesia for performing a spinal cord compression lesion is 2.5-3% isoflurane in air...
  40. doi Treatment of ocular surface injuries by limbal and mesenchymal stem cells growing on nanofiber scaffolds
    Alena Zajicova
    Institute of Molecular Genetics, Academy of Sciences, Prague, Czech Republic
    Cell Transplant 19:1281-90. 2010
    ..The results thus show that nanofibers prepared from copolymer PA6/12 represent a convenient scaffold for growth of LSCs and MSCs and transfer to treat SC deficiencies and various ocular surface injuries...
  41. doi Flt3 ligand synergizes with granulocyte-colony-stimulating factor in bone marrow mobilization to improve functional outcome after spinal cord injury in the rat
    Lucia Urdzíková
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Cytotherapy 13:1090-104. 2011
    ..The effect of granulocyte-colony-stimulating factor (G-CSF) and/or the cytokine fms-like thyrosin kinase 3 (Flt3) ligand on functional outcome and tissue regeneration was studied in a rat model of spinal cord injury (SCI)...
  42. pmc Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury
    Vaclav Vanecek
    Institute of Experimental Medicine AS CR, Prague, Czech Republic
    Int J Nanomedicine 7:3719-30. 2012
    ..Such magnetic systems with tunable geometric parameters may provide the additional level of control needed to enhance the efficiency of stem cell delivery in spinal cord injury...
  43. doi Transplantation of predifferentiated adipose-derived stromal cells for the treatment of spinal cord injury
    David Arboleda
    Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, v v i, Videnska 1083, 142 20 Prague 4, Czech Republic
    Cell Mol Neurobiol 31:1113-22. 2011
    ....
  44. ncbi Three-dimensional confocal morphometry reveals structural changes in astrocyte morphology in situ
    Alexandr Chvatal
    Department of Neurobiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Neurosci Res 85:260-71. 2007
    ..Our data show that astrocytes in brain slices undergoing hypotonic stress display cell volume regulation as well as transient changes in morphology...
  45. ncbi Analysis of K+ accumulation reveals privileged extracellular region in the vicinity of glial cells in situ
    Alexandr Chvatal
    Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Neurosci Res 78:668-82. 2004
    ..The presence of such privileged regions around cells in the CNS may affect the accumulation and diffusion of other neuroactive substances and alter communication between cells in the CNS...
  46. doi Metabolic changes in the thalamus after spinal cord injury followed by proton MR spectroscopy
    Katarina Likavcanova
    Institute of Experimental Medicine, ASCR, Prague, Czech Republic
    Magn Reson Med 59:499-506. 2008
    ....
  47. pmc The Anti-Inflammatory Compound Curcumin Enhances Locomotor and Sensory Recovery after Spinal Cord Injury in Rats by Immunomodulation
    Lucia Machova Urdzikova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 142 20, Czech Republic
    Int J Mol Sci 17:. 2015
    ..These results, therefore, demonstrate that curcumin has a profound anti-inflammatory therapeutic potential in the treatment of spinal cord injury, especially when given immediately after the injury. ..
  48. pmc Life on magnets: stem cell networking on micro-magnet arrays
    Vitalii Zablotskii
    Institute of Physics AS CR, v v i, Prague, Czech Republic
    PLoS ONE 8:e70416. 2013
    ....
  49. pmc Astrocytes and glutamate homoeostasis in Alzheimer's disease: a decrease in glutamine synthetase, but not in glutamate transporter-1, in the prefrontal cortex
    Magdalena Kulijewicz-Nawrot
    Institute of Experimental Medicine, ASCR, Videnska 1083, 142 20 Prague, Czech Republic
    ASN Neuro 5:273-82. 2013
    ....
  50. pmc Controlled gentamicin release from multi-layered electrospun nanofibrous structures of various thicknesses
    Jakub Sirc
    Department of Polymer Gels, Institute of Macromolecular Chemistry, Academy of Science of the Czech Republic, Prague, Czech Republic
    Int J Nanomedicine 7:5315-25. 2012
    ..Both experiments proved that the released gentamicin retained its activity and showed that the retention of the drug in the nanofibers was prolonged with the increasing thickness of the covering layers...
  51. doi Oxidative damage to biological macromolecules in human bone marrow mesenchymal stromal cells labeled with various types of iron oxide nanoparticles
    Bozena Novotna
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Toxicol Lett 210:53-63. 2012
    ..Lowering intracellular label concentrations and authenticating oxidative stress levels using in vivo experiments are required to ensure the safety of SPIONs for biomedical applications...
  52. doi Properties and growth of human bone marrow mesenchymal stromal cells cultivated in different media
    Karolina Turnovcova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Cytotherapy 11:874-85. 2009
    ..Therefore, we tested the influence of several culture media on colony formation, population doubling (PD) time, cell cycle and surface marker expression...
  53. pmc Injectable Extracellular Matrix Hydrogels as Scaffolds for Spinal Cord Injury Repair
    Dmitry Tukmachev
    1 Institute of Experimental Medicine AS CR, Prague, Czech Republic
    Tissue Eng Part A 22:306-17. 2016
    ..However, fast hydrogel degradation might be a limiting factor for the use of native ECM hydrogels in the treatment of acute SCI. ..
  54. doi Physiology of Ca(2+) signalling in stem cells of different origins and differentiation stages
    Oksana Forostyak
    Institute of Experimental Medicine, Czech Academy of Sciences, Videnska 1083, Prague 14220, Czech Republic Department of Neuroscience, Charles University, Second Faculty of Medicine, V Uvalu 84, 15006 Prague, Czech Republic Electronic address
    Cell Calcium 59:57-66. 2016
    ..Together, identification and assignment of a unique ion channel and a Ca(2+) handling footprint for each cell type would be necessary to qualify them as physiologically suitable for medical research, drug screening, and cell therapy...
  55. doi Sodium-calcium exchanger and R-type Ca(2+) channels mediate spontaneous [Ca(2+)]i oscillations in magnocellular neurones of the rat supraoptic nucleus
    Stepan Kortus
    Department of Molecular Neurophysiology, Institute of Experimental Medicine, Czech Academy of Sciences, Videnska 1083, 14220 Prague, Czech Republic Institute of Physiology, Czech Academy of Sciences, Videnska 1083, 14220 Prague, Czech Republic Institute of Biophysics and Informatics, First Medical Faculty, Charles University in Prague, Salmovska 1, 12000 Prague, Czech Republic
    Cell Calcium 59:289-98. 2016
    ..In conclusion, spontaneous oscillations in magnocellular neurones are mediated by a concerted action of R-type Ca(2+) channels and the NCX fluctuating between forward and reverse modes. ..
  56. doi Suppression of alkali-induced oxidative injury in the cornea by mesenchymal stem cells growing on nanofiber scaffolds and transferred onto the damaged corneal surface
    Jitka Cejkova
    Institute of Experimental Medicine, Laboratory of Eye Histochemistry and Pharmacology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague 4, Czech Republic Electronic address
    Exp Eye Res 116:312-23. 2013
    ..This resulted in reduced corneal inflammation as well as neovascularization and significantly accelerated corneal healing. ..
  57. ncbi Diffusion parameters of the extracellular space in human gliomas
    Lydia Vargova
    Department of Neuroscience, Charles University, Second Medical Faculty, Prague, Czech Republic
    Glia 42:77-88. 2003
    ..Tumor malignancy grade strongly corresponds to an increase in ECS volume, which is accompanied by a change in ECS structure manifested by an increase in diffusion barriers for small molecules...
  58. doi Co-transplantation of olfactory ensheathing glia and mesenchymal stromal cells does not have synergistic effects after spinal cord injury in the rat
    Takashi Amemori
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 142 20, Prague, Czech Republic
    Cytotherapy 12:212-25. 2010
    ..Both facilitate functional improvement after SCI by producing trophic factors and cytokines. In this study, the co-transplantation of both types of cells was studied to clarify their additive and/ or synergistic effects on SCI...
  59. doi Analysis of in vitro and in vivo characteristics of human embryonic stem cell-derived neural precursors
    Nataliya Kozubenko
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Cell Transplant 19:471-86. 2010
    ..These results complete the characterization of the CCTL14 line of hESCs and provide a framework for developing cell selection strategies for neural cell-based therapies...
  60. doi Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering
    Sarka Kubinova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Biomaterials 30:4601-9. 2009
    ..In contrast, MSCs spread and proliferated on P(HEMA-CHLMA) and LN-P(HEMA-CHLMA) hydrogels...
  61. doi Mesenchymal stromal cells prolong the lifespan in a rat model of amyotrophic lateral sclerosis
    Serhiy Forostyak
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Cytotherapy 13:1036-46. 2011
    ....
  62. ncbi Magnetic resonance tracking of human CD34+ progenitor cells separated by means of immunomagnetic selection and transplanted into injured rat brain
    Pavla Jendelova
    Institute of Experimental Medicine ASCR, Prague, Czech Republic
    Cell Transplant 14:173-82. 2005
    ..The fact that microbeads coated with different commercially available antibodies can bind to specific cell types opens extensive possibilities for cell tracking in vivo...
  63. doi A comparative study of three different types of stem cells for treatment of rat spinal cord injury
    Jiri Ruzicka
    Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic
    Cell Transplant . 2016
    ..The iPS-NP treatment of spinal cord injury (SCI) provided the highest recovery of locomotor function due to robust graft survival, and its effect on tissue sparing, reduction of glial scarring and increased axonal sprouting...
  64. doi SIKVAV-modified highly superporous PHEMA scaffolds with oriented pores for spinal cord injury repair
    Sarka Kubinova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Tissue Eng Regen Med 9:1298-309. 2015
    ....
  65. ncbi Decreased proliferation in the adult rat hippocampus after exposure to the Morris water maze and its reversal by fluoxetine
    Katerina Namestkova
    Department of Neuroscience and Center for Cell Therapy and Tissue Repair, Charles University, 2nd Medical Faculty, Prague, Czech Republic
    Behav Brain Res 163:26-32. 2005
    ....
  66. doi Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression
    Vladimir Holan
    Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, 142 20 Prague, Czech Republic
    J Control Release 156:406-12. 2011
    ..These results suggest that CsA-loaded electrospun nanofibers can serve as effective drug carriers for the local/topical suppression of an inflammatory reaction and simultaneously could be used as scaffolds for cell-based therapy...
  67. doi MR tracking of stem cells in living recipients
    Eva Sykova
    Institute of Experimental Medicine ASCR, Prague, Czech Republic
    Methods Mol Biol 549:197-215. 2009
    ....
  68. doi Surface modification of hydrogels based on poly(2-hydroxyethyl methacrylate) with extracellular matrix proteins
    Eduard Brynda
    Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, 162 06, Prague 6, Czech Republic
    J Mater Sci Mater Med 20:909-15. 2009
    ..The modification with collagen and particularly with collagen and laminin or fibronectin allowed the adhesion and growth of mesenchymal stromal cells and astrocytes on the hydrogel surface...
  69. ncbi Methods for behavioral testing of spinal cord injured rats
    Jiri Sedy
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic
    Neurosci Biobehav Rev 32:550-80. 2008
    ..This review is primarily targeted towards researchers outside the field or to researchers new to the field of SCI...
  70. doi Nanofibers prepared by needleless electrospinning technology as scaffolds for wound healing
    Michal Dubsky
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    J Mater Sci Mater Med 23:931-41. 2012
    ..The results show that gelatin nanofibers produced by needleless technology accelerate wound healing and may be suitable as a scaffold for cell transfer and skin regeneration...
  71. doi The use of superporous Ac-CGGASIKVAVS-OH-modified PHEMA scaffolds to promote cell adhesion and the differentiation of human fetal neural precursors
    Sarka Kubinova
    Institute of Experimental Medicine AS CR, vvi, Prague, Czech Republic
    Biomaterials 31:5966-75. 2010
    ..The Ac-CGGASIKVAVS-OH modification of superporous P(HEMA-AEMA) hydrogels improves cell adhesive properties and promotes neural stem cell differentiation...
  72. ncbi A new model of severe neurogenic pulmonary edema in spinal cord injured rat
    Jiri Sedy
    Institute of Experimental Medicine, ASCR, Prague, Czech Republic
    Neurosci Lett 423:167-71. 2007
    ..However, if very severe neurogenic pulmonary edema needs to be evoked, spinal cord injury under 1.5% isoflurane anesthesia could be used, but one-third of the animals will be lost...
  73. doi Biomaterials combined with cell therapy for treatment of spinal cord injury
    Sarka Kubinova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Regen Med 7:207-24. 2012
    ....
  74. ncbi Voltage-dependent potassium currents in hypertrophied rat astrocytes after a cortical stab wound
    Miroslava Anderova
    Department of Neuroscience, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Glia 48:311-26. 2004
    ....
  75. doi The correlation between 1H MRS choline concentrations and MR diffusion trace values in human brain tumors
    Dita Wagnerova
    MR Unit, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
    MAGMA 22:19-31. 2009
    ....
  76. pmc Diffusion in brain extracellular space
    Eva Sykova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Physiol Rev 88:1277-340. 2008
    ..Knowledge of ECS diffusion properties is valuable in contexts ranging from understanding extrasynaptic volume transmission to the development of paradigms for drug delivery to the brain...
  77. doi Nanotechnologies in regenerative medicine
    Sarka Kubinova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Minim Invasive Ther Allied Technol 19:144-56. 2010
    ..g, the central nervous system, heart, liver and kidney and also in pancreatic islet and stem cell transplantation...
  78. doi Atropine may prevent the development of neurogenic pulmonary edema
    Jiri Sedy
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, Czech Republic
    Med Hypotheses 73:42-4. 2009
    ..v. immediately after the maintenance of the living functions, intubation and ability to monitor patient...
  79. ncbi Macroporous hydrogels based on 2-hydroxyethyl methacrylate. Part 5: hydrolytically degradable materials
    Martin Pradny
    Academy of Sciences of the Czech Republic, Institute of Macromolecular Chemistry, 16206, Prague 6, Czech Republic
    J Mater Sci Mater Med 17:1357-64. 2006
    ..The process of degradation was followed gravimetrically and by optical and electron microscopy. In vivo biological tests with hydrogels based on copolymers of 2-ethoxyethyl methacrylate/N-(2-hydroxypropyl)methacrylamide were performed...
  80. doi Human galectins induce conversion of dermal fibroblasts into myofibroblasts and production of extracellular matrix: potential application in tissue engineering and wound repair
    Barbora Dvořánková
    Institute of Anatomy, 1st Faculty of Medicine, Charles University, Prague, Czech Republic
    Cells Tissues Organs 194:469-80. 2011
    ..In conclusion, we describe the differential potential of certain human galectins to induce the conversion of dermal fibroblasts into MFBs and the generation of a bioactive cell culture substratum...
  81. doi Bral1: "Superglue" for the extracellular matrix in the brain white matter
    Michal Cicanic
    Department of Neuroscience and Center for Cell Therapy and Tissue Repair, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague 5, Czech Republic
    Int J Biochem Cell Biol 44:596-9. 2012
    ..Recent studies suggest its function in promoting saltatory neural conduction. This article reviews the current knowledge about the structure, expression and function of this link protein...
  82. doi Nanotechnology for treatment of stroke and spinal cord injury
    Sarka Kubinova
    Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague, Czech Republic
    Nanomedicine (Lond) 5:99-108. 2010
    ..The incorporation of biologically active epitopes and/or the utilization of these scaffolds as stem cell carriers may further enhance their therapeutic efficacy...
  83. ncbi Metabolite and diffusion changes in the rat brain after Leksell Gamma Knife irradiation
    Vit Herynek
    MR Unit, Radiology Department, Institute for Clinical and Experimental Medicine, Prague, Czech Republic
    Magn Reson Med 52:397-402. 2004
    ..Histology confirmed the MRI data, showing either scar formation or the development of a postnecrotic cavity...