Hongkai Wu

Summary

Affiliation: Tsinghua University
Country: China

Publications

  1. ncbi request reprint Generation of complex, static solution gradients in microfluidic channels
    Hongkai Wu
    Department of Chemistry, Tsinghua University, Beijing, China
    J Am Chem Soc 128:4194-5. 2006
  2. doi request reprint Convenient formation of nanoparticle aggregates on microfluidic chips for highly sensitive SERS detection of biomolecules
    Jianhua Zhou
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Anal Bioanal Chem 402:1601-9. 2012
  3. pmc Freestanding 3-D microvascular networks made of alginate hydrogel as a universal tool to create microchannels inside hydrogels
    Chong Hu
    Department of Chemistry, The Hong Kong Baptist University, Waterloo Rd, Kowloon, Hong Kong, China
    Biomicrofluidics 10:044112. 2016
  4. doi request reprint A microfluidic circulatory system integrated with capillary-assisted pressure sensors
    Yangfan Chen
    Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China
    Lab Chip . 2017
  5. doi request reprint Highly emissive and biocompatible dopamine-derived oligomers as fluorescent probes for chemical detection and targeted bioimaging
    Bin Xiong
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Chem Commun (Camb) 50:13578-80. 2014
  6. doi request reprint Materials for microfluidic chip fabrication
    Kangning Ren
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Acc Chem Res 46:2396-406. 2013
  7. ncbi request reprint A one-step strategy for ultra-fast and low-cost mass production of plastic membrane microfluidic chips
    Chong Hu
    Department of Chemistry, Hong Kong Baptist University, Waterloo Rd, Kowloon, Hong Kong, China
    Lab Chip 16:3909-3918. 2016
  8. doi request reprint Pumping-induced perturbation of flow in microfluidic channels and its implications for on-chip cell culture
    Jianhua Zhou
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Lab Chip 11:2288-94. 2011
  9. doi request reprint Centrifugation-Assisted Single-Cell Trapping in a Truncated Cone-Shaped Microwell Array Chip for the Real-Time Observation of Cellular Apoptosis
    Lu Huang
    Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China
    Anal Chem 87:12169-76. 2015
  10. doi request reprint Recent developments in microfluidics for cell studies
    Bin Xiong
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Adv Mater 26:5525-32. 2014

Collaborators

  • Dik Lung Ma
  • Jing Su
  • Kangning Ren
  • Bo Huang
  • Yangfan Chen
  • Yin Chen
  • Jianhua Zhou
  • Chong Hu
  • Bin Xiong
  • Richard N Zare
  • Lu Huang
  • Han Sun
  • Ho Nam Chan
  • Yiwei Shu
  • Samuel Kim
  • Bo Shen
  • Wen Dai
  • Yihua Zhao
  • Hui Yan
  • Brian K Kobilka
  • Yiqi Luo
  • Yusheng Shen
  • Sean A Michael
  • Qian Tian
  • Wanbo Li
  • Sheng Lin
  • Zhengzhi Liu
  • Chiu Wing Chan
  • Chung Hang Leung
  • Xiaoxi Chen
  • George M Whitesides
  • Xiangping Qian
  • Junli Zhou
  • Yaozong Chen
  • Declan Ryan
  • Boyang Zhang
  • Arthur Grossman
  • Sebastien Granier
  • Devaki Bhaya
  • Chengde Mao
  • Steven J Metallo
  • Michael N Liang
  • Insung S Choi

Detail Information

Publications21

  1. ncbi request reprint Generation of complex, static solution gradients in microfluidic channels
    Hongkai Wu
    Department of Chemistry, Tsinghua University, Beijing, China
    J Am Chem Soc 128:4194-5. 2006
    ..The gradient profile in the channel can be designed to have an arbitrary form (within the range of the existing gradient in the hydrogel) by controlling the local direction of the channel at each point...
  2. doi request reprint Convenient formation of nanoparticle aggregates on microfluidic chips for highly sensitive SERS detection of biomolecules
    Jianhua Zhou
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Anal Bioanal Chem 402:1601-9. 2012
    ..With our microfluidic chip, the detection limit of Raman can reach as low as the picomolar level, comparable to that of normal mass spectrometry...
  3. pmc Freestanding 3-D microvascular networks made of alginate hydrogel as a universal tool to create microchannels inside hydrogels
    Chong Hu
    Department of Chemistry, The Hong Kong Baptist University, Waterloo Rd, Kowloon, Hong Kong, China
    Biomicrofluidics 10:044112. 2016
    ..The method is readily implementable to applications in vascular tissue regeneration, drug discovery, 3-D culture, etc. ..
  4. doi request reprint A microfluidic circulatory system integrated with capillary-assisted pressure sensors
    Yangfan Chen
    Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China
    Lab Chip . 2017
    ..Human umbilical vein endothelial cells (HUVECs) were cultured on chip and cells can respond to mechanical forces generated by arterial-like flow patterns...
  5. doi request reprint Highly emissive and biocompatible dopamine-derived oligomers as fluorescent probes for chemical detection and targeted bioimaging
    Bin Xiong
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Chem Commun (Camb) 50:13578-80. 2014
    ..We prepared highly emissive and biocompatible dopamine-derived oligomers, and demonstrated their applications as novel fluorescent probes for sensitive detection of Fe(3+) ions and targeted bioimaging in live cells. ..
  6. doi request reprint Materials for microfluidic chip fabrication
    Kangning Ren
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Acc Chem Res 46:2396-406. 2013
    ..Also, significant expanding of commercial applications can be predicted...
  7. ncbi request reprint A one-step strategy for ultra-fast and low-cost mass production of plastic membrane microfluidic chips
    Chong Hu
    Department of Chemistry, Hong Kong Baptist University, Waterloo Rd, Kowloon, Hong Kong, China
    Lab Chip 16:3909-3918. 2016
    ..Due to the high speed and low cost of the fabrication process, this single-step method will facilitate the mass production of microfluidic chips and commercialization of microfluidic technologies...
  8. doi request reprint Pumping-induced perturbation of flow in microfluidic channels and its implications for on-chip cell culture
    Jianhua Zhou
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Lab Chip 11:2288-94. 2011
    ..We also examine the possible effects of these large perturbations on NIH 3T3 cells in microfluidic channels and find that they could cause the detachment of NIH 3T3 cells in the microchannels...
  9. doi request reprint Centrifugation-Assisted Single-Cell Trapping in a Truncated Cone-Shaped Microwell Array Chip for the Real-Time Observation of Cellular Apoptosis
    Lu Huang
    Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, China
    Anal Chem 87:12169-76. 2015
    ..This simple, robust, and efficient approach possesses great potential in diverse applications, such as drug screening, biosensing, and fundamental biological research. ..
  10. doi request reprint Recent developments in microfluidics for cell studies
    Bin Xiong
    Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Adv Mater 26:5525-32. 2014
    ..Major advancements in microfluidic device fabrication and the growing trend of implementing microfluidics in cell studies are presented, with a focus on biological research and clinical diagnostics. ..
  11. doi request reprint Generation of alginate microfibers with a roller-assisted microfluidic system
    Jing Su
    Department of Chemistry, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China
    Lab Chip 9:996-1001. 2009
    ..In addition, various functional materials (colloidal particles, bacteria and nanoparticles) have been encapsulated into the gel fibers with this technique...
  12. doi request reprint Chemical cytometry on microfluidic chips
    Hui Yan
    Department of Chemistry, The Hong Kong University of Science and Technology, Hong Kong, P R China
    Electrophoresis 29:1775-86. 2008
    ..This review focuses on the development of relevant components and their integration for on-chip chemical cytometry...
  13. ncbi request reprint Arrays of self-assembled monolayers for studying inhibition of bacterial adhesion
    Xiangping Qian
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Anal Chem 74:1805-10. 2002
    ....
  14. ncbi request reprint Fabrication of complex three-dimensional microchannel systems in PDMS
    Hongkai Wu
    Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138, USA
    J Am Chem Soc 125:554-9. 2003
    ..The channels can be used as templates to form 3D structures in other materials...
  15. ncbi request reprint Coating of poly(dimethylsiloxane) with n-dodecyl-beta-D-maltoside to minimize nonspecific protein adsorption
    Bo Huang
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Lab Chip 5:1005-7. 2005
    ..Poly(dimethylsiloxane)(PDMS) surface is coated with n-dodecyl-beta-D-maltoside, which reduces the nonspecifically adsorbed protein on the PDMS surface to the single molecule level...
  16. ncbi request reprint Construction of microfluidic chips using polydimethylsiloxane for adhesive bonding
    Hongkai Wu
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Lab Chip 5:1393-8. 2005
    ..The major advantages of this bonding method are its generality and its ease of use...
  17. ncbi request reprint Phospholipid biotinylation of polydimethylsiloxane (PDMS) for protein immobilization
    Bo Huang
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Lab Chip 6:369-73. 2006
    ..We demonstrate the immobilization of biotinylated antibodies and lectins through biotin-avidin interactions...
  18. ncbi request reprint Controlling electroosmotic flow in poly(dimethylsiloxane) separation channels by means of prepolymer additives
    Yiqi Luo
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Anal Chem 78:4588-92. 2006
    ..To demonstrate these effects, we show that the electrophoretic separation of immunocomplexes in free solution can be readily accomplished in a microfluidic chip made of UDA-doped (0.5 wt %) PDMS with a dynamic coating of DDM...
  19. ncbi request reprint Counting low-copy number proteins in a single cell
    Bo Huang
    Department of Chemistry, Stanford University, Stanford, CA 94305 5080, USA
    Science 315:81-4. 2007
    ..PCC 7942) and observed marked differences in the levels of specific complexes in cell populations that were grown under nitrogen-depleted conditions...
  20. ncbi request reprint Use of a mixture of n-dodecyl-beta-D-maltoside and sodium dodecyl sulfate in poly(dimethylsiloxane) microchips to suppress adhesion and promote separation of proteins
    Bo Huang
    Department of Chemistry, Stanford University, Stanford, California 94305 5080, USA
    Anal Chem 79:9145-9. 2007
    ..1% (w/v) DDM and 0.03% (w/v) SDS. We have also analyzed the composition of light-harvesting protein-chromophore complexes in cyanobacteria...
  21. ncbi request reprint A prototype two-dimensional capillary electrophoresis system fabricated in poly(dimethylsiloxane)
    Xiaoxi Chen
    Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, USA
    Anal Chem 74:1772-8. 2002
    ..This work suggests one type of design that might form the basis for a microfabricated device for 2D capillary electrophoresis...