Genomes and Genes
J M Nicholls
Affiliation: The University of Hong Kong
- The use of sialidase therapy for respiratory viral infectionsJohn M Nicholls
Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong
Antiviral Res 98:401-9. 2013..Continued evaluation of the roles and activities of bacterial sialidases is required to expand the range of successful antiviral therapies targeting Sia or its derivatives...
- Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methodsJohn M Nicholls
Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China
J Virol Methods 179:409-13. 2012..This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde...
- Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distributionSjouke G M Van Poucke
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
Virol J 7:38. 2010..Human (Sia alpha2-6Gal) and avian virus receptors (Sia alpha2-3Gal) were identified with Sambucus Nigra and Maackia amurensis lectins respectively...
- Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cellsMichael C W Chan
Departments of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, PR China
Respir Res 10:102. 2009..These are polarized cells and the polarity of influenza virus entry and egress as well as the secretion of cytokines and chemokines from the virus infected cells are likely to be central to the pathogenesis of human H5N1 disease...
- Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cellsM C W Chan
Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region of China
Respir Res 6:135. 2005..Since respiratory epithelial cells are the primary target cell for replication of influenza viruses, it is pertinent to investigate the cytokine induction profile of H5N1 viruses in these cells...
- Tropism of avian influenza A (H5N1) in the upper and lower respiratory tractJ M Nicholls
Department of Pathology, University of Hong Kong, Pok Fu Lam Road, Hong Kong SAR, China
Nat Med 13:147-9. 2007..We now demonstrate that ex vivo cultures of human nasopharyngeal, adenoid and tonsillar tissues can be infected with H5N1 viruses in spite of an apparent lack of these receptors...
- Avian influenza: update on pathogenesis and laboratory diagnosisJohn M Nicholls
Departments of Pathology and Microbiology, The University of Hong Kong, Hong Kong
Respirology 13:S14-8. 2008..In this review we highlight the similarities and differences of SARS and H5N1, and emphasize the importance of appropriate sampling for laboratory diagnosis of the latter...
- Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza virusesJohn M Nicholls
Pathology Department, The University of Hong Kong, Pok Fu Lam, Hong Kong, Hong Kong SAR
Respir Res 8:73. 2007....
- Anatomical pathology is dead? Long live anatomical pathologyJohn M Nicholls
Department of Pathology, The University of Hong Kong, Queen Mary Hospital Compound, Hong Kong
Pathology 43:635-41. 2011..Though proteomic techniques will become more prevalent in the future, it will need the expertise of an anatomical pathologist to dissect out and validate this added information...
- Time course and cellular localization of SARS-CoV nucleoprotein and RNA in lungs from fatal cases of SARSJohn M Nicholls
Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China
PLoS Med 3:e27. 2006..To our knowledge, there have been no comprehensive studies investigating viral infection at the cellular level in humans...
- SARS: clinical virology and pathogenesisJohn Nicholls
Department of Pathology, The University of Hong Kong, China
Respirology 8:S6-8. 2003..After 10 days of illness the picture changes to one of organizing DAD with increased fibrosis, squamous metaplasia and multinucleated giant cells. The role of cytokines in the pathogenesis of SARS is still unclear...
- Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in childrenK H Chan
Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, Hong Kong SAR, China
J Clin Virol 42:65-9. 2008..The quality of clinical specimens is a crucial determinant for virological diagnosis...
- The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particlesY L Siu
HKU Pasteur Research Centre, 8 Sassoon Road, Hong Kong SAR, China
J Virol 82:11318-30. 2008..Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress...
- The aetiology, origins, and diagnosis of severe acute respiratory syndromeL L M Poon
Department of Microbiology, University of Hong Kong, Hong Kong SAR, China
Lancet Infect Dis 4:663-71. 2004..However, the low viral load in the respiratory tract makes early diagnosis of SARS a diagnostic challenge, although improvements in the sensitivity of molecular diagnostic methods continue to be made...
- Evolving complexities of influenza virus and its receptorsJohn M Nicholls
Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR
Trends Microbiol 16:149-57. 2008..Understanding these additional components is important in comprehending the infection and the transmission of both existing human influenza viruses and newly emerging avian influenza viruses...
- Influenza H5N1 and H1N1 virus replication and innate immune responses in bronchial epithelial cells are influenced by the state of differentiationRenee W Y Chan
Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, People s Republic of China
PLoS ONE 5:e8713. 2010..Since influenza infection in the bronchial epithelium will lead to tissue damage and associate with the epithelium regeneration, the data generated from the undifferentiated NHBE cultures may also be relevant to disease pathogenesis...
- Lung pathology of fatal severe acute respiratory syndromeJohn M Nicholls
Department of Pathology, University of Hong Kong, Hong Kong Special Administrative Region, China
Lancet 361:1773-8. 2003..Severe acute respiratory syndrome (SARS) is a novel infectious disease with global impact. A virus from the family Coronaviridae has been identified as the cause, but the pathogenesis is still unclear...
- Tumor suppressor Alpha B-crystallin (CRYAB) associates with the cadherin/catenin adherens junction and impairs NPC progression-associated propertiesZ Huang
Department of Clinical Oncology and Center for Cancer Research, University of Hong Kong, Hong Kong SAR, PR China
Oncogene 31:3709-20. 2012..Taken together, these results indicate that CRYAB functions to suppress NPC progression by associating with the cadherin/catenin adherens junction and modulating the β-catenin function...
- Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptationOlive T W Li
State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
PLoS ONE 4:e5658. 2009..These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern...
- Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cellsHelen K W Law
Department of Paediatrics and Adolescent Medicine, Hong Kong Jockey Club Clinical Research Centre, Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China
Blood 106:2366-74. 2005..The lack of antiviral cytokine response against a background of intense chemokine up-regulation could represent a mechanism of immune evasion by SARS-CoV...
- DAS181 inhibits H5N1 influenza virus infection of human lung tissuesRenee W Y Chan
Department of Microbiology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR
Antimicrob Agents Chemother 53:3935-41. 2009..These findings support the potential value of DAS181 as a broad-spectrum therapeutic agent against influenza viruses, especially H5N1...
- The association of E-cadherin expression and the methylation status of the E-cadherin gene in nasopharyngeal carcinoma cellsS W Tsao
Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong, SAR, China
Eur J Cancer 39:524-31. 2003..Our results also suggest that reducing the methylation of the E-cad gene may be a potential therapeutic strategy for NPC...
- Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tractMichael C W Chan
Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China
Am J Pathol 176:1828-40. 2010..These findings are therefore relevant for understanding transmission and therapy...
- Implications of endocrine gland-derived vascular endothelial growth factor/prokineticin-1 signaling in human neuroblastoma progressionElly S W Ngan
Department of Surgery, University of Hong Kong, Pokfulam, Hong Kong, SAR, China, and Department of Pediatric Surgery, The Second Affiliated Hospital of China Medical University, Shenyang, China
Clin Cancer Res 13:868-75. 2007..In this report, we further elucidate its role in neuroblastoma progression...
- Avian influenza H5-containing virus-like particles (VLPs): host-cell receptor specificity by STD NMR spectroscopyThomas Haselhorst
Institute for Glycomics, Griffith University, Gold Coast Campus, Queensland, 4222, Australia
Angew Chem Int Ed Engl 47:1910-2. 2008
- Severe acute respiratory syndrome-associated coronavirus nucleocapsid protein interacts with Smad3 and modulates transforming growth factor-beta signalingXingang Zhao
State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
J Biol Chem 283:3272-80. 2008..Our results reveal a novel mode of Smad3 action in a Smad4-independent manner and may lead to successful strategies for SARS treatment by targeting the TGF-beta signaling molecules...
- Hyperinduction of cyclooxygenase-2-mediated proinflammatory cascade: a mechanism for the pathogenesis of avian influenza H5N1 infectionSuki M Y Lee
Department of Microbiology, Siriraj Hospital, Bangkok, Thailand
J Infect Dis 198:525-35. 2008..These data provide a basis for the possible development of novel therapeutic interventions for the treatment of H5N1 disease, as adjuncts to antiviral drugs...
- Comment on: concerns of using sialidase fusion protein as an experimental drug to combat seasonal and pandemic influenzaJohn M Nicholls
J Antimicrob Chemother 62:426-8; author reply 428-9. 2008
- Influenza A H5N1 replication sites in humansMongkol Uiprasertkul
Mahidol University, Bangkok, Thailand
Emerg Infect Dis 11:1036-41. 2005..In contrast to disseminated infection documented in other mammals and birds, H5N1 viral replication in humans may be restricted to the lung and intestine, and the major site of H5N1 viral replication in the lung is the pneumocyte...