J M Nicholls

Summary

Affiliation: The University of Hong Kong
Country: China

Publications

  1. doi request reprint The use of sialidase therapy for respiratory viral infections
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong
    Antiviral Res 98:401-9. 2013
  2. doi request reprint Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China
    J Virol Methods 179:409-13. 2012
  3. pmc Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution
    Sjouke G M Van Poucke
    Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Virol J 7:38. 2010
  4. pmc Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells
    Michael C W Chan
    Departments of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, PR China
    Respir Res 10:102. 2009
  5. pmc Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells
    M C W Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region of China
    Respir Res 6:135. 2005
  6. ncbi request reprint Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract
    J M Nicholls
    Department of Pathology, University of Hong Kong, Pok Fu Lam Road, Hong Kong SAR, China
    Nat Med 13:147-9. 2007
  7. doi request reprint Avian influenza: update on pathogenesis and laboratory diagnosis
    John M Nicholls
    Departments of Pathology and Microbiology, The University of Hong Kong, Hong Kong
    Respirology 13:S14-8. 2008
  8. pmc Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses
    John M Nicholls
    Pathology Department, The University of Hong Kong, Pok Fu Lam, Hong Kong, Hong Kong SAR
    Respir Res 8:73. 2007
  9. doi request reprint Anatomical pathology is dead? Long live anatomical pathology
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Queen Mary Hospital Compound, Hong Kong
    Pathology 43:635-41. 2011
  10. pmc Time course and cellular localization of SARS-CoV nucleoprotein and RNA in lungs from fatal cases of SARS
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China
    PLoS Med 3:e27. 2006

Collaborators

  • Leo Lm Poon
  • George Sai Wah Tsao
  • C M Chan
  • Glenn D Francis
  • Yi Guan
  • Z Huang
  • F M F Cheung
  • Xiao Ping Dong
  • Y Cheng
  • W Y Chan
  • S S Chiu
  • H K Law
  • Wilina Lim
  • Ronald B Moss
  • Yu Liu
  • Elly S W Ngan
  • Yu Lung Lau
  • Jagdish W Butany
  • P Auewarakul
  • Renee W Y Chan
  • Michael C W Chan
  • J S Malik Peiris
  • W H Chui
  • Carol C C Ho
  • Kit M Yuen
  • S W Tsao
  • Wendy C L Yu
  • J S M Peiris
  • Mongkol Uiprasertkul
  • Sjouke G M Van Poucke
  • Alan D L Sihoe
  • Olive T W Li
  • Thomas Haselhorst
  • K H Chan
  • Suki M Y Lee
  • Xingang Zhao
  • Y L Siu
  • M C W Chan
  • Lynsia L S Tang
  • Hans J Nauwynck
  • David S H Wong
  • Kristien Van Reeth
  • Wico W Lai
  • Joseph S M Peiris
  • Amy C Y Lo
  • Dora L W Kwong
  • Joanne H M Fong
  • Cynthia S W Leung
  • C K Lo
  • Fang Fang
  • Rositsa Karamanska
  • Adam C N Wong
  • Gallen Triana-Baltzer
  • Anne Dell
  • Stuart M Haslam
  • Qixiang Li
  • Chung Yan Cheung
  • J Lo
  • George L Tipoe
  • Mark von Itzstein
  • Jean Michel Garcia
  • Jimmy C C Lai
  • K T Teoh
  • Nancy Y Ip
  • Kenrie P Y Hui
  • F Kien
  • Faith J Rose
  • R Altmeyer
  • R Bruzzone
  • Ye Guang Chen
  • B Nal
  • Tasneem Islam
  • Connie Y H Leung
  • N Escriou
  • Kulkanya Chokephaibulkit
  • R W Y Chan
  • Kanittar Srisook
  • C Y Cheung
  • H T Long
  • Y O Chan
  • Nirun Vanprapar
  • Pilaipan Puthavathana
  • Phisanu Pooruk
  • Kantima Sangsiriwut
  • Malik Peiris
  • S Y Leung
  • Y C Wong
  • Xianghong Wang
  • A L M Cheung
  • P W Yuen

Detail Information

Publications30

  1. doi request reprint The use of sialidase therapy for respiratory viral infections
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong
    Antiviral Res 98:401-9. 2013
    ..Continued evaluation of the roles and activities of bacterial sialidases is required to expand the range of successful antiviral therapies targeting Sia or its derivatives...
  2. doi request reprint Detection of highly pathogenic influenza and pandemic influenza virus in formalin fixed tissues by immunohistochemical methods
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China
    J Virol Methods 179:409-13. 2012
    ..This monoclonal antibody is recommended for routine diagnostic use for the detection of influenza A infected tissues that have been fixed in formalin or paraformaldehyde...
  3. pmc Replication of avian, human and swine influenza viruses in porcine respiratory explants and association with sialic acid distribution
    Sjouke G M Van Poucke
    Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, 9820 Merelbeke, Belgium
    Virol J 7:38. 2010
    ..Human (Sia alpha2-6Gal) and avian virus receptors (Sia alpha2-3Gal) were identified with Sambucus Nigra and Maackia amurensis lectins respectively...
  4. pmc Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells
    Michael C W Chan
    Departments of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Hong Kong SAR, PR China
    Respir Res 10:102. 2009
    ..These are polarized cells and the polarity of influenza virus entry and egress as well as the secretion of cytokines and chemokines from the virus infected cells are likely to be central to the pathogenesis of human H5N1 disease...
  5. pmc Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells
    M C W Chan
    Department of Microbiology, The University of Hong Kong, Queen Mary Hospital, Hong Kong Special Administrative Region of China
    Respir Res 6:135. 2005
    ..Since respiratory epithelial cells are the primary target cell for replication of influenza viruses, it is pertinent to investigate the cytokine induction profile of H5N1 viruses in these cells...
  6. ncbi request reprint Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract
    J M Nicholls
    Department of Pathology, University of Hong Kong, Pok Fu Lam Road, Hong Kong SAR, China
    Nat Med 13:147-9. 2007
    ..We now demonstrate that ex vivo cultures of human nasopharyngeal, adenoid and tonsillar tissues can be infected with H5N1 viruses in spite of an apparent lack of these receptors...
  7. doi request reprint Avian influenza: update on pathogenesis and laboratory diagnosis
    John M Nicholls
    Departments of Pathology and Microbiology, The University of Hong Kong, Hong Kong
    Respirology 13:S14-8. 2008
    ..In this review we highlight the similarities and differences of SARS and H5N1, and emphasize the importance of appropriate sampling for laboratory diagnosis of the latter...
  8. pmc Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses
    John M Nicholls
    Pathology Department, The University of Hong Kong, Pok Fu Lam, Hong Kong, Hong Kong SAR
    Respir Res 8:73. 2007
    ....
  9. doi request reprint Anatomical pathology is dead? Long live anatomical pathology
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Queen Mary Hospital Compound, Hong Kong
    Pathology 43:635-41. 2011
    ..Though proteomic techniques will become more prevalent in the future, it will need the expertise of an anatomical pathologist to dissect out and validate this added information...
  10. pmc Time course and cellular localization of SARS-CoV nucleoprotein and RNA in lungs from fatal cases of SARS
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR, China
    PLoS Med 3:e27. 2006
    ..To our knowledge, there have been no comprehensive studies investigating viral infection at the cellular level in humans...
  11. ncbi request reprint SARS: clinical virology and pathogenesis
    John Nicholls
    Department of Pathology, The University of Hong Kong, China
    Respirology 8:S6-8. 2003
    ..After 10 days of illness the picture changes to one of organizing DAD with increased fibrosis, squamous metaplasia and multinucleated giant cells. The role of cytokines in the pathogenesis of SARS is still unclear...
  12. doi request reprint Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children
    K H Chan
    Department of Microbiology, The University of Hong Kong and Queen Mary Hospital, Hong Kong SAR, China
    J Clin Virol 42:65-9. 2008
    ..The quality of clinical specimens is a crucial determinant for virological diagnosis...
  13. pmc The M, E, and N structural proteins of the severe acute respiratory syndrome coronavirus are required for efficient assembly, trafficking, and release of virus-like particles
    Y L Siu
    HKU Pasteur Research Centre, 8 Sassoon Road, Hong Kong SAR, China
    J Virol 82:11318-30. 2008
    ..Fluorescent VLPs will be used further to investigate the role of cellular machineries during SARS-CoV egress...
  14. ncbi request reprint The aetiology, origins, and diagnosis of severe acute respiratory syndrome
    L L M Poon
    Department of Microbiology, University of Hong Kong, Hong Kong SAR, China
    Lancet Infect Dis 4:663-71. 2004
    ..However, the low viral load in the respiratory tract makes early diagnosis of SARS a diagnostic challenge, although improvements in the sensitivity of molecular diagnostic methods continue to be made...
  15. doi request reprint Evolving complexities of influenza virus and its receptors
    John M Nicholls
    Department of Pathology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR
    Trends Microbiol 16:149-57. 2008
    ..Understanding these additional components is important in comprehending the infection and the transmission of both existing human influenza viruses and newly emerging avian influenza viruses...
  16. pmc Influenza H5N1 and H1N1 virus replication and innate immune responses in bronchial epithelial cells are influenced by the state of differentiation
    Renee W Y Chan
    Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong SAR, People s Republic of China
    PLoS ONE 5:e8713. 2010
    ..Since influenza infection in the bronchial epithelium will lead to tissue damage and associate with the epithelium regeneration, the data generated from the undifferentiated NHBE cultures may also be relevant to disease pathogenesis...
  17. ncbi request reprint Lung pathology of fatal severe acute respiratory syndrome
    John M Nicholls
    Department of Pathology, University of Hong Kong, Hong Kong Special Administrative Region, China
    Lancet 361:1773-8. 2003
    ..Severe acute respiratory syndrome (SARS) is a novel infectious disease with global impact. A virus from the family Coronaviridae has been identified as the cause, but the pathogenesis is still unclear...
  18. doi request reprint Tumor suppressor Alpha B-crystallin (CRYAB) associates with the cadherin/catenin adherens junction and impairs NPC progression-associated properties
    Z Huang
    Department of Clinical Oncology and Center for Cancer Research, University of Hong Kong, Hong Kong SAR, PR China
    Oncogene 31:3709-20. 2012
    ..Taken together, these results indicate that CRYAB functions to suppress NPC progression by associating with the cadherin/catenin adherens junction and modulating the β-catenin function...
  19. pmc Full factorial analysis of mammalian and avian influenza polymerase subunits suggests a role of an efficient polymerase for virus adaptation
    Olive T W Li
    State Key Laboratory of Emerging Infectious Diseases, Department of Microbiology, The University of Hong Kong, Hong Kong SAR, China
    PLoS ONE 4:e5658. 2009
    ..These results suggested that the viral polymerase activity might be relevant for the genesis of influenza viruses of human health concern...
  20. pmc Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells
    Helen K W Law
    Department of Paediatrics and Adolescent Medicine, Hong Kong Jockey Club Clinical Research Centre, Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, Pokfulam, Hong Kong, China
    Blood 106:2366-74. 2005
    ..The lack of antiviral cytokine response against a background of intense chemokine up-regulation could represent a mechanism of immune evasion by SARS-CoV...
  21. pmc DAS181 inhibits H5N1 influenza virus infection of human lung tissues
    Renee W Y Chan
    Department of Microbiology, The University of Hong Kong, Pok Fu Lam, Hong Kong SAR
    Antimicrob Agents Chemother 53:3935-41. 2009
    ..These findings support the potential value of DAS181 as a broad-spectrum therapeutic agent against influenza viruses, especially H5N1...
  22. ncbi request reprint The association of E-cadherin expression and the methylation status of the E-cadherin gene in nasopharyngeal carcinoma cells
    S W Tsao
    Department of Anatomy, Faculty of Medicine, University of Hong Kong, Hong Kong, SAR, China
    Eur J Cancer 39:524-31. 2003
    ..Our results also suggest that reducing the methylation of the E-cad gene may be a potential therapeutic strategy for NPC...
  23. pmc Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract
    Michael C W Chan
    Department of Microbiology, Queen Mary Hospital, Pokfulam, Hong Kong SAR, China
    Am J Pathol 176:1828-40. 2010
    ..These findings are therefore relevant for understanding transmission and therapy...
  24. ncbi request reprint Implications of endocrine gland-derived vascular endothelial growth factor/prokineticin-1 signaling in human neuroblastoma progression
    Elly S W Ngan
    Department of Surgery, University of Hong Kong, Pokfulam, Hong Kong, SAR, China, and Department of Pediatric Surgery, The Second Affiliated Hospital of China Medical University, Shenyang, China
    Clin Cancer Res 13:868-75. 2007
    ..In this report, we further elucidate its role in neuroblastoma progression...
  25. doi request reprint Avian influenza H5-containing virus-like particles (VLPs): host-cell receptor specificity by STD NMR spectroscopy
    Thomas Haselhorst
    Institute for Glycomics, Griffith University, Gold Coast Campus, Queensland, 4222, Australia
    Angew Chem Int Ed Engl 47:1910-2. 2008
  26. ncbi request reprint Severe acute respiratory syndrome-associated coronavirus nucleocapsid protein interacts with Smad3 and modulates transforming growth factor-beta signaling
    Xingang Zhao
    State Key Laboratory of Biomembrane and Membrane Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China
    J Biol Chem 283:3272-80. 2008
    ..Our results reveal a novel mode of Smad3 action in a Smad4-independent manner and may lead to successful strategies for SARS treatment by targeting the TGF-beta signaling molecules...
  27. doi request reprint Hyperinduction of cyclooxygenase-2-mediated proinflammatory cascade: a mechanism for the pathogenesis of avian influenza H5N1 infection
    Suki M Y Lee
    Department of Microbiology, Siriraj Hospital, Bangkok, Thailand
    J Infect Dis 198:525-35. 2008
    ..These data provide a basis for the possible development of novel therapeutic interventions for the treatment of H5N1 disease, as adjuncts to antiviral drugs...
  28. doi request reprint Comment on: concerns of using sialidase fusion protein as an experimental drug to combat seasonal and pandemic influenza
    John M Nicholls
    J Antimicrob Chemother 62:426-8; author reply 428-9. 2008
  29. pmc Influenza A H5N1 replication sites in humans
    Mongkol Uiprasertkul
    Mahidol University, Bangkok, Thailand
    Emerg Infect Dis 11:1036-41. 2005
    ..In contrast to disseminated infection documented in other mammals and birds, H5N1 viral replication in humans may be restricted to the lung and intestine, and the major site of H5N1 viral replication in the lung is the pneumocyte...