Xiaofeng Zheng

Summary

Affiliation: Peking University
Country: China

Publications

  1. ncbi request reprint Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004
    Hui Ren
    Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 60:1292-4. 2004
  2. pmc The herpes simplex virus 1 UL36USP deubiquitinase suppresses DNA repair in host cells via deubiquitination of proliferating cell nuclear antigen.
    Xiaodong Dong
    J Biol Chem 292:8472-8483. 2017
  3. pmc Tip-to-tip interaction in the crystal packing of PACSIN 2 is important in regulating tubulation activity
    Xiaoyun Bai
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China
    Protein Cell 4:695-701. 2013
  4. doi request reprint RNF168-mediated H2A neddylation antagonizes ubiquitylation of H2A and regulates DNA damage repair
    Tingting Li
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    J Cell Sci 127:2238-48. 2014
  5. pmc Restructuring of the dinucleotide-binding fold in an NADP(H) sensor protein
    Xiaofeng Zheng
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 104:8809-14. 2007
  6. ncbi request reprint Protein expression, crystallization and preliminary X-ray crystallographic studies on HSCARG from Homo sapiens
    Xueyu Dai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, P R China
    Protein Pept Lett 13:955-7. 2006
  7. doi request reprint NADPH is an allosteric regulator of HSCARG
    Xueyu Dai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    J Mol Biol 387:1277-85. 2009
  8. pmc HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation
    Bin Hu
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    Nucleic Acids Res 42:5582-93. 2014
  9. pmc Loss of zygotic NUP107 protein causes missing of pharyngeal skeleton and other tissue defects with impaired nuclear pore function in zebrafish embryos
    Xiaofeng Zheng
    State Key Laboratory of Biomembrane and Membrane Engineering, Tsinghua Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
    J Biol Chem 287:38254-64. 2012
  10. pmc Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity
    Geng Meng
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
    J Biol Chem 285:39898-904. 2010

Collaborators

Detail Information

Publications52

  1. ncbi request reprint Protein preparation, crystallization and preliminary X-ray analysis of human adrenal gland protein AD-004
    Hui Ren
    Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 60:1292-4. 2004
    ..The crystals belong to space group P6(1) or P6(5), with unit-cell parameters a = b = 99.56, c = 57.19 A. A complete 2.0 A data set has been collected at a rotating-anode X-ray source and structure determination is under way...
  2. pmc The herpes simplex virus 1 UL36USP deubiquitinase suppresses DNA repair in host cells via deubiquitination of proliferating cell nuclear antigen.
    Xiaodong Dong
    J Biol Chem 292:8472-8483. 2017
    ..These findings suggest a role of UL36USP in the DNA damage-response pathway...
  3. pmc Tip-to-tip interaction in the crystal packing of PACSIN 2 is important in regulating tubulation activity
    Xiaoyun Bai
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, China
    Protein Cell 4:695-701. 2013
    ....
  4. doi request reprint RNF168-mediated H2A neddylation antagonizes ubiquitylation of H2A and regulates DNA damage repair
    Tingting Li
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    J Cell Sci 127:2238-48. 2014
    ..Our findings elucidate the relationship of H2A ubiquitylation and neddylation, and suggest a novel modulatory approach to DNA damage repair through the neddylation pathway. ..
  5. pmc Restructuring of the dinucleotide-binding fold in an NADP(H) sensor protein
    Xiaofeng Zheng
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 104:8809-14. 2007
    ..One of the functions regulated by HSCARG may be argininosuccinate synthetase that is involved in NO synthesis...
  6. ncbi request reprint Protein expression, crystallization and preliminary X-ray crystallographic studies on HSCARG from Homo sapiens
    Xueyu Dai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, P R China
    Protein Pept Lett 13:955-7. 2006
    ..4 A on Mar225 CCD Detector at SER-CAT 22BM synchrotron source. The crystals belong to F23 space group, with unit cell parameters a=b=c=223.30A, alpha=beta=gamma=90 degrees . There are two molecules per asymmetry unit...
  7. doi request reprint NADPH is an allosteric regulator of HSCARG
    Xueyu Dai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    J Mol Biol 387:1277-85. 2009
    ..This equilibrium, in turn, will tip the dimerization balance of the protein molecule and consequently controls the regulatory function of HSCARG...
  8. pmc HSCARG, a novel regulator of H2A ubiquitination by downregulating PRC1 ubiquitin E3 ligase activity, is essential for cell proliferation
    Bin Hu
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    Nucleic Acids Res 42:5582-93. 2014
    ..This study unravels a novel mechanism for the regulation of H2A ubiquitination and elucidates how regulators of H2A ubiquitination affect cell cycle. ..
  9. pmc Loss of zygotic NUP107 protein causes missing of pharyngeal skeleton and other tissue defects with impaired nuclear pore function in zebrafish embryos
    Xiaofeng Zheng
    State Key Laboratory of Biomembrane and Membrane Engineering, Tsinghua Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China
    J Biol Chem 287:38254-64. 2012
    ..Our findings shed new light on developmental function of Nup107 in vertebrates...
  10. pmc Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity
    Geng Meng
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
    J Biol Chem 285:39898-904. 2010
    ..Our results demonstrate the saccharide-binding property of SI-CLP by structure and in vitro biochemical analyses and suggest the possible roles of SI-CLP in pathogen sensing and endotoxin neutralization...
  11. pmc Structural and functional characterization of K339T substitution identified in the PB2 subunit cap-binding pocket of influenza A virus
    Yong Liu
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
    J Biol Chem 288:11013-23. 2013
    ..It is informative to track variations in the cap-binding pocket of PB2 in surveillance of the evolution and spread of influenza virus...
  12. ncbi request reprint Protein expression, crystallization and preliminary X-ray crystallographic studies of YjbK from Bacillus subtilis
    Xue Yu Dai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, China
    Protein Pept Lett 12:663-4. 2005
    ..0 A in-house. The crystals belong to P1 space group, with unit cell parameters a = 32.38 A, b = 34.69 A, c = 46.02 A, alpha = 96.560 degrees, beta = 99.683 degrees, gamma = 111.333 degrees. There is one molecule per asymmetric unit...
  13. pmc Crystallization and X-ray crystallographic analysis of the cap-binding domain of influenza A virus H1N1 polymerase subunit PB2
    Yong Liu
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, 100871, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 69:280-3. 2013
    ..32 Å. The crystal symmetry belongs to space group P1 with unit-cell parameters a=29.49, b=37.04, c=38.33 Å, α=71.10, β=69.84, γ=75.85°. There is one molecule in the asymmetric unit...
  14. pmc Rigidity of wedge loop in PACSIN 3 protein is a key factor in dictating diameters of tubules
    Xiaoyun Bai
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
    J Biol Chem 287:22387-96. 2012
    ..Thus, the rigidity of the wedge loop is a key factor in dictating tubule diameters...
  15. ncbi request reprint Crystal structure of Thermoanaerobacter tengcongensis hypoxanthine-guanine phosphoribosyl transferase L160I mutant--insights into inhibitor design
    Qiang Chen
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, China
    FEBS J 274:4408-15. 2007
    ..7 A resolution. Surprisingly, the active site is occupied by a peptide from the N-terminus of a neighboring tetramer. These crystal contacts suggest an alternate strategy for structure-based inhibitor design...
  16. doi request reprint Depletion of hCINAP by RNA interference causes defects in Cajal body formation, histone transcription, and cell viability
    Jinfang Zhang
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, China
    Cell Mol Life Sci 67:1907-18. 2010
    ..Altogether, these data suggest that hCINAP is essential for the formation of canonical CBs, histone transcription, and cell viability...
  17. pmc The crystal structure of human adenylate kinase 6: An adenylate kinase localized to the cell nucleus
    Hui Ren
    National Laboratory of Protein Engineering and Plant Genetic Engineering and Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
    Proc Natl Acad Sci U S A 102:303-8. 2005
    ..The identification of a nuclear-localized AK sheds light on nucleotide metabolism in the nucleus and the energetic communication between mitochondria and nucleus by means of phosphotransfer networks...
  18. pmc Preparation, crystallization and preliminary X-ray analysis of YjcG protein from Bacillus subtilis
    Dan Li
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:496-8. 2005
    ..Crystals suitable for X-ray analysis were obtained that diffracted to 2.3 A and belonged to space group C2, with unit-cell parameters a = 99.66, b = 73.93, c = 61.77 A, beta = 113.56 degrees...
  19. ncbi request reprint Crystallization and preliminary X-ray crystallographic studies on SI-CLP, a novel human Glyco_18 domain-containing protein
    Geng Meng
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, PR China
    Protein Pept Lett 16:336-8. 2009
    ..7 A. The crystals belong to the space group P3(2)21 with unit cell parameters a=b=99.79 A, c=250.53 A, alpha=beta=90 degrees, gamma=120 degrees. There are two molecules per asymmetry unit...
  20. ncbi request reprint A novel nuclear-localized protein with special adenylate kinase properties from Caenorhabditis elegans
    Ruitong Zhai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    FEBS Lett 580:3811-7. 2006
    ..These results suggest that ADLP is more likely a member of the AK6 family. Furthermore, RNAi experiments targeting ADLP were conducted and showed that RNAi treatment resulted in the suppression of worm growth...
  21. ncbi request reprint Identification of a novel nuclear-localized adenylate kinase from Drosophila melanogaster
    Geng Meng
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Beijing, China
    Biochemistry (Mosc) 73:38-43. 2008
    ..Therefore, based on the substrate specificity, the specific nuclear localization in the cell, and the sequence similarity with human AK6, we named this novel adenylate kinase identified from the fly DAK6...
  22. doi request reprint A chemical lipid modification of recombinant preS antigen to study the mechanism of HBV attachment to the host cell
    Bin Chen
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    J Biotechnol 137:8-13. 2008
    ....
  23. doi request reprint An NADPH sensor protein (HSCARG) down-regulates nitric oxide synthesis by association with argininosuccinate synthetase and is essential for epithelial cell viability
    Yanmei Zhao
    National Laboratory of Protein Engineering and Plant Genetic Engineering and Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
    J Biol Chem 283:11004-13. 2008
    ..These results indicate that HSCARG regulation of AS activity is crucial for maintaining the intracellular balance between redox state and nitric oxide levels...
  24. ncbi request reprint Soluble expression, purification, and stabilization of a pro-apoptotic human protein, CARP
    Qiang Chen
    Department of Biochemistry and Molecular Biology, College of Life Sciences, Peking University, Beijing 100871, China
    Protein Expr Purif 45:329-34. 2006
    ..Successfully growing crystals of the purified CARP protein also proved that we can produce well folded CARP protein in E. coli...
  25. doi request reprint Human secreted stabilin-1-interacting chitinase-like protein aggravates the inflammation associated with rheumatoid arthritis and is a potential macrophage inflammatory regulator in rodents
    Weichun Xiao
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China
    Arthritis Rheumatol 66:1141-52. 2014
    ..To study the relationship between the human secreted protein stabilin-1-interacting chitinase-like protein (SI-CLP) and rheumatoid arthritis (RA)...
  26. pmc HSCARG inhibits NADPH oxidase activity through regulation of the expression of p47phox
    Weichun Xiao
    State Key Lab of Protein and Plant Gene Research, Beijing, China
    PLoS ONE 8:e59301. 2013
    ..Furthermore, HSCARG regulates p47phox through inhibition of NF-κB activity. Our findings identify HSCARG as a novel regulator in regulation of the activity of NADPH oxidase and ROS homeostasis...
  27. doi request reprint A CRM1-dependent nuclear export signal controls nucleocytoplasmic translocation of HSCARG, which regulates NF-κB activity
    Mei Zhang
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
    Traffic 13:790-9. 2012
    ..These results indicate that nucleocytoplasmic translocation of HSCARG plays an important role in fine-tuning NF-κB signaling...
  28. pmc HSCARG regulates NF-kappaB activation by promoting the ubiquitination of RelA or COMMD1
    Min Lian
    Department of Biochemistry and Molecular Biology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    J Biol Chem 284:17998-8006. 2009
    ..Thus, NF-kappaB activation was terminated efficiently. Our results indicate that HSCARG plays critical roles in regulation of NF-kappaB in response to cellular redox changes by promoting ubiquitination and proteolysis of RelA or COMMD1...
  29. doi request reprint HSCARG inhibits activation of NF-kappaB by interacting with IkappaB kinase-beta
    Qini Gan
    Department of Biochemistry and Molecular Biology, Peking University, Beijing 100871, China
    J Cell Sci 122:4081-8. 2009
    ..In addition, our data indicate that IKKbeta is required for HSCARG-inhibited NF-kappaB activation. Our findings delineate a pathway by which HSCARG negatively regulates NF-kappaB activation...
  30. ncbi request reprint Identification of the critical regions in hepatitis B virus preS required for its stability
    Min Lian
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing, 100871, China
    J Pept Sci 14:307-12. 2008
    ..Moreover, the availability of large amounts of well-folded and stable preS1-120 enables us to carry out further structural determination and mechanistic study on HBV infection...
  31. ncbi request reprint Alternative IMP binding in feedback inhibition of hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis
    Qiang Chen
    Laboratory of Structural Biology, College of Life Sciences, Peking University, Beijing 100871, People s Republic of China
    J Mol Biol 348:1199-210. 2005
    ..tengcongensis HGPRT is compared with those of mesophilic HPRTs, and several possible features contributing to its thermostability are elucidated. Overall, T.tengcongensis HGPRT appears to be more diverged from other PRTs...
  32. ncbi request reprint A nonsynonymous SNP in human cytosolic sialidase in a small Asian population results in reduced enzyme activity: potential link with severe adverse reactions to oseltamivir
    Chuan Yun Li
    Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Cell Res 17:357-62. 2007
    ..We propose that this Asian-enriched sialidase variation caused by the SNP, likely in homozygous form, may be associated with certain severe adverse reactions to oseltamivir...
  33. pmc Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients
    Min Lian
    National Laboratory of Protein Engineering and Plant Genetic Engineering, Peking University, Beijing 100871, China
    Virol J 4:93. 2007
    ..The S antigen (HBsAg) seroconversion is much less frequent. Since HBeAg can be negative in the presence of high viral replication, preS antigen (HBpreSAg) might be a useful indicator in management of chronic HBV infection...
  34. pmc Crystallization and preliminary X-ray crystallographic analysis of human PACSIN 1 protein
    Xiaoyun Bai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:73-5. 2010
    ..65, b = 87.38, c = 91.76 A, alpha = 90.00, beta = 113.61, gamma = 90.00 degrees . There were two molecules in the asymmetric unit and the solvent content was estimated to be about 70.47%...
  35. pmc Protein expression, crystallization and preliminary X-ray crystallographic studies of LidA from Legionella pneumophila
    Wenzhuang Zhu
    State Key Lab of Protein and Plant Gene Research, Peking University, Beijing 100871, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 67:637-9. 2011
    ..75 Å. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 57.5, b = 64.5, c = 167.3 Å, α = β = γ = 90°. There is one molecule per asymmetric unit...
  36. pmc Crystallization and preliminary X-ray crystallographic studies of O-methyltransferase from Anabaena PCC 7120
    Guoming Li
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:1039-41. 2009
    ..The crystals belonged to space group C222(1), with unit-cell parameters a = 131.620, b = 227.994, c = 150.777 A, alpha = beta = gamma = 90 degrees . There are eight molecules per asymmetric unit...
  37. pmc The ATPase hCINAP regulates 18S rRNA processing and is essential for embryogenesis and tumour growth
    Dongmei Bai
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China
    Nat Commun 7:12310. 2016
    ..Our results connect the role of hCINAP in ribosome assembly with tumorigenesis. Modulation of hCINAP expression may be a promising target for cancer therapy. ..
  38. pmc Positive selection for the male functionality of a co-retroposed gene in the hominoids
    Yong Zhang
    Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, 100871, PR China
    BMC Evol Biol 9:252. 2009
    ..However, understanding of the origination and biological function of PIPSL requires determination of the coding potential of this gene as well as the evolutionary forces acting on its hominoid copies...
  39. pmc Cloning, purification, crystallization and preliminary X-ray diffraction analysis of mouse PACSIN 3 protein
    Xiaoyun Bai
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, People s Republic of China
    Acta Crystallogr Sect F Struct Biol Cryst Commun 68:159-62. 2012
    ..6 Å resolution and belonged to space group P2(1), with unit-cell parameters a = 46.9, b = 54.7, c = 193.7 Å, α = 90, β = 96.9, γ = 90°. These data should provide further information on PACSIN-family protein structures...
  40. ncbi request reprint Protein preparation, crystallization and preliminary X-ray analysis of imidazolonepropionase from Bacillus subtilis
    Yamei Yu
    The National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, PR China
    Biochim Biophys Acta 1764:153-6. 2006
    ..The best crystal diffracted to 2.0 A and belonged to the space group P2(1) with unit-cell parameters a = 57.73 A, b = 106.34 A, c = 66.47 A, beta = 89.93 degrees ...
  41. doi request reprint MyD88 NEDDylation negatively regulates MyD88-dependent NF-κB signaling through antagonizing its ubiquitination
    Fangxue Yan
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    Biochem Biophys Res Commun 482:632-637. 2017
    ..Taken together, this study reveals that NEDDylation of MyD88 regulates NF-κB activity through antagonizing its ubiquitination, suggesting a novel mechanism of modulating NF-κB signaling pathway...
  42. pmc HSCARG negatively regulates the cellular antiviral RIG-I like receptor signaling pathway by inhibiting TRAF3 ubiquitination via recruiting OTUB1
    Yanyan Peng
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing, China
    PLoS Pathog 10:e1004041. 2014
    ..This study demonstrated that HSCARG is a negative regulator that enables balanced antiviral innate immunity. ..
  43. pmc Regulation of the HDM2-p53 pathway by ribosomal protein L6 in response to ribosomal stress
    Dongmei Bai
    State Key Lab of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing 100871, China and Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    Nucleic Acids Res 42:1799-811. 2014
    ..Together, our study identifies the crucial function of RPL6 in regulating HDM2-p53 pathway, which highlights the importance of RPL6 in human genetic diseases and cancers. ..
  44. ncbi request reprint Systematic high-yield production of human secreted proteins in Escherichia coli
    Xueyu Dai
    National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China
    Biochem Biophys Res Commun 332:593-601. 2005
    ..These recombinant proteins were characterized as well-folded proteins. This system enables us to rapidly obtain soluble and highly purified human secreted proteins for further functional studies...
  45. pmc A human-specific de novo protein-coding gene associated with human brain functions
    Chuan Yun Li
    Center for Bioinformatics, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing, China
    PLoS Comput Biol 6:e1000734. 2010
    ..FLJ33706 provided the strongest evidence so far that human-specific de novo genes can have protein-coding potential and differential protein expression, and be involved in human brain functions...
  46. doi request reprint hCINAP negatively regulates NF-κB signaling by recruiting the phosphatase PP1 to deactivate IKK complex
    Linglong Qu
    State Key Lab of Protein and Plant Gene Research, Beijing 100871, China Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China
    J Mol Cell Biol 7:529-42. 2015
    ..Our study suggests a novel mechanism underlying deactivation of IKK and provides new insight into the negative regulation of NF-κB signaling. ..
  47. pmc The crystal structure of the PB2 cap-binding domain of influenza B virus reveals a novel cap recognition mechanism
    Yong Liu
    From the State Key Lab of Protein and Plant Gene Research and Department of Biochemistry and Molecular Biology, School of Life Sciences, Peking University, Beijing 100871, China and
    J Biol Chem 290:9141-9. 2015
    ..This study indicated that FluB PB2cap has a unique cap recognition mechanism compared with FluA PB2cap, providing molecular insight into inhibitor design targeting FluB PB2cap. ..
  48. doi request reprint The crystal structure of human UDP-glucose pyrophosphorylase reveals a latch effect that influences enzymatic activity
    Quan Yu
    State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, P R China
    Biochem J 442:283-91. 2012
    ..Mutagenesis studies showed that both dissociation of octamers and mutations of the latch loop can significantly affect the UGPase activity. Moreover, this latch effect is also evolutionarily meaningful in UGPase from different species...
  49. doi request reprint Structure determination of human Fas apoptosis inhibitory molecule and identification of the critical residues linking the interdomain interaction to the anti-apoptotic activity
    Guoming Li
    State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing 100871, People s Republic of China
    Acta Crystallogr D Biol Crystallogr 70:1812-22. 2014
    ..Moreover, mutations of these critical residues also interfered in the anti-apoptotic activity of FAIM-S. Thus, the structural and functional data presented here will provide insight into the anti-apoptotic mechanism of FAIM-S. ..
  50. pmc Interruption of cenph causes mitotic failure and embryonic death, and its haploinsufficiency suppresses cancer in zebrafish
    Xinyi Zhao
    Protein Science Laboratory of the Ministry of the Education, College of Life Sciences, Tsinghua University, Beijing 100084, China
    J Biol Chem 285:27924-34. 2010
    ..Taken together, our findings uncover an essential role of cenph in mitosis and embryonic development and its association with tumor development...
  51. pmc Activation of the protein kinase PKR by short double-stranded RNAs with single-stranded tails
    Xiaofeng Zheng
    Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA
    RNA 10:1934-45. 2004
    ..The ss-dsRNA motif occurs in a variety of cellular and viral RNAs, suggesting possible novel functions for PKR in nature...
  52. pmc Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways
    Xiaofeng Zheng
    Departments of Cell and Molecular Biology and Molecular Medicine, Karolinska Institutet, 171 77 Stockholm, Sweden
    Proc Natl Acad Sci U S A 105:3368-73. 2008
    ..In conclusion, the data reveal posttranslational modification of the activated form of the Notch receptor and an intricate mode of cross-coupling between the Notch and hypoxia signaling pathways...