Ying Jin Yuan

Summary

Affiliation: Nankai University
Country: China

Publications

  1. doi request reprint Lipidomic analysis reveals differential defense responses of Taxus cuspidata cells to two elicitors, methyl jasmonate and cerium (Ce4+)
    Song Yang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Biochim Biophys Acta 1781:123-34. 2008
  2. ncbi request reprint Isolation of differential genes in suspension cultures of Taxus cuspidata induced by additional taxol
    Ying Jin Yuan
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PR China
    Mol Biotechnol 20:137-43. 2002
  3. ncbi request reprint Aqueous extract of Salvia miltiorrhiza attenuates increased endothelial permeability induced by tumor necrosis factor-alpha
    Mei Ding
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    Int Immunopharmacol 5:1641-51. 2005
  4. doi request reprint Integrated phospholipidomics and transcriptomics analysis of Saccharomyces cerevisiae with enhanced tolerance to a mixture of acetic acid, furfural, and phenol
    Jie Yang
    Key Laboratory of Systems Bioengineering, Ministry of Education Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    OMICS 16:374-86. 2012
  5. doi request reprint Metabolomic analysis of the positive effects on Ketogulonigenium vulgare growth and 2-keto-L-gulonic acid production by reduced glutathione
    Jian Zhou
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    OMICS 16:387-96. 2012
  6. pmc Proteomic research reveals the stress response and detoxification of yeast to combined inhibitors
    Ming Zhu Ding
    Key Laboratory of Systems Bioengineering, Ministry of Education Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    PLoS ONE 7:e43474. 2012
  7. doi request reprint High temperature aqueous ammonia pretreatment and post-washing enhance the high solids enzymatic hydrolysis of corn stover
    Lei Qin
    Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Bioresour Technol 146:504-11. 2013
  8. doi request reprint Metabolomic analysis reveals key metabolites related to the rapid adaptation of Saccharomyce cerevisiae to multiple inhibitors of furfural, acetic acid, and phenol
    Xin Wang
    Key Laboratory of Systems Bioengineering, Ministry of Education Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    OMICS 17:150-9. 2013
  9. pmc Proteomic analysis of Ketogulonicigenium vulgare under glutathione reveals high demand for thiamin transport and antioxidant protection
    Qian Ma
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    PLoS ONE 7:e32156. 2012
  10. doi request reprint Comparative metabolomic study of Penicillium chrysogenum during pilot and industrial penicillin fermentations
    Ming Zhu Ding
    Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    Appl Biochem Biotechnol 168:1223-38. 2012

Collaborators

Detail Information

Publications88

  1. doi request reprint Lipidomic analysis reveals differential defense responses of Taxus cuspidata cells to two elicitors, methyl jasmonate and cerium (Ce4+)
    Song Yang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Biochim Biophys Acta 1781:123-34. 2008
    ..These results demonstrate that differential alternation of glycerolphospholipids caused by phospholipases constitutes an important step in cell death response to Ce(4+) and increasing taxol production...
  2. ncbi request reprint Isolation of differential genes in suspension cultures of Taxus cuspidata induced by additional taxol
    Ying Jin Yuan
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PR China
    Mol Biotechnol 20:137-43. 2002
    ..Among them, TIGT13 and TIGT21 were apparently homogeneous with apbE and carbamoylphosphate synthetase, respectively. Other cDNA fragments showed no significant analogy with the known sequences in GenBank...
  3. ncbi request reprint Aqueous extract of Salvia miltiorrhiza attenuates increased endothelial permeability induced by tumor necrosis factor-alpha
    Mei Ding
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    Int Immunopharmacol 5:1641-51. 2005
    ..Data from this study suggest that one of the mechanisms S. miltiorrhiza exerts its pharmacological effect is through its modulation of endothelial cell permeability...
  4. doi request reprint Integrated phospholipidomics and transcriptomics analysis of Saccharomyces cerevisiae with enhanced tolerance to a mixture of acetic acid, furfural, and phenol
    Jie Yang
    Key Laboratory of Systems Bioengineering, Ministry of Education Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    OMICS 16:374-86. 2012
    ....
  5. doi request reprint Metabolomic analysis of the positive effects on Ketogulonigenium vulgare growth and 2-keto-L-gulonic acid production by reduced glutathione
    Jian Zhou
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    OMICS 16:387-96. 2012
    ..vulgare and 2KGA production. Furthermore, it also provides new insights into the possible cellular interaction between Bacillus species and K. vulgare...
  6. pmc Proteomic research reveals the stress response and detoxification of yeast to combined inhibitors
    Ming Zhu Ding
    Key Laboratory of Systems Bioengineering, Ministry of Education Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    PLoS ONE 7:e43474. 2012
    ....
  7. doi request reprint High temperature aqueous ammonia pretreatment and post-washing enhance the high solids enzymatic hydrolysis of corn stover
    Lei Qin
    Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Bioresour Technol 146:504-11. 2013
    ..The increasing concentration of glucose and ligno-phenolics mainly inhibited the enzymatic hydrolysis of aqueous ammonia pretreated corn stover...
  8. doi request reprint Metabolomic analysis reveals key metabolites related to the rapid adaptation of Saccharomyce cerevisiae to multiple inhibitors of furfural, acetic acid, and phenol
    Xin Wang
    Key Laboratory of Systems Bioengineering, Ministry of Education Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    OMICS 17:150-9. 2013
    ..This study provides new insights into the tolerance mechanism against multiple inhibitors, and guides for the improvement of tolerant ethanologenic yeast strains for lignocellulose-bioethanol fermentation...
  9. pmc Proteomic analysis of Ketogulonicigenium vulgare under glutathione reveals high demand for thiamin transport and antioxidant protection
    Qian Ma
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    PLoS ONE 7:e32156. 2012
    ..megaterium cells lysed from our former research results. This study reveals the demand for transmembrane transport of substrates, especially thiamin, and the demand for antioxidant protection of K. vulgare...
  10. doi request reprint Comparative metabolomic study of Penicillium chrysogenum during pilot and industrial penicillin fermentations
    Ming Zhu Ding
    Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    Appl Biochem Biotechnol 168:1223-38. 2012
    ..More generally, this study attempts to address the scarcity of studies that contrast the metabolic outcomes between commercial- and pilot-scale conditions...
  11. doi request reprint Insights into the roles of exogenous glutamate and proline in improving streptolydigin production of Streptomyces lydicus with metabolomic analysis
    Jing Sheng Cheng
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin, 300072, People s Republic of China
    J Ind Microbiol Biotechnol 40:1303-14. 2013
    ..Taken together, exogenous glutamate and proline not only directly provided the precursors of streptolydigin biosynthesis, but also might alter the metabolic homeostasis of S. lydicus E9 during improving the production of streptolydigin. ..
  12. pmc Optimization of CDT-1 and XYL1 expression for balanced co-production of ethanol and xylitol from cellobiose and xylose by engineered Saccharomyces cerevisiae
    Jian Zha
    Key Laboratory of Systems Bioengineering, Tianjin University, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    PLoS ONE 8:e68317. 2013
    ..Thus, we achieved a balanced co-fermentation of cellobiose (0.165 g/L/h) and xylose (0.162 g/L/h) at similar rates to co-produce ethanol (0.36 g/g) and xylitol (1.00 g/g). ..
  13. ncbi request reprint Direct proteomic mapping of Streptomyces Luteogriseus Strain 103 and cnn1 and insights into antibiotic biosynthesis
    Yu Xia Wang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    J Proteome Res 4:1999-2006. 2005
    ..The activity inhibition of beta-ketoacyl ACP synthase, a key enzyme in the polyketide pathway, directly resulted in the decrease of Maituolaimysin production, which proved the conclusion obtained in the proteomic research...
  14. ncbi request reprint Antioxidant responses to oleic acid in two-liquid-phase suspension cultures of Taxus cuspidata
    Qiu Man Xu
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China
    Appl Biochem Biotechnol 125:11-26. 2005
    ....
  15. doi request reprint Evaluation of storage methods for the conversion of corn stover biomass to sugars based on steam explosion pretreatment
    Zhi Hua Liu
    Key Laboratory of Systems Bioengineering, Ministry of Education, Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Bioresour Technol 132:5-15. 2013
    ..Therefore, the wet storage methods would be desirable for the conversion of corn stover biomass to fermentable sugars based on steam explosion pretreatment and enzymatic hydrolysis...
  16. doi request reprint Inoculum size-dependent interactive regulation of metabolism and stress response of Saccharomyces cerevisiae revealed by comparative metabolomics
    Ming Zhu Ding
    Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Nankai District, Tianjin 300072, PR China
    J Biotechnol 144:279-86. 2009
    ..These findings provided new insights into characterizing the metabolic regulation and stress response depending on inoculum density during ethanol fermentation...
  17. doi request reprint Integrative investigation of lipidome and signal pathways in human endothelial cells under oxidative stress
    Jie Yang
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, P R China
    Mol Biosyst 7:2428-40. 2011
    ..This combined approach has enabled future investigations for possible therapeutic interventions in phospholipids and cPLA(2) activity for defense against oxidative cellular stress...
  18. doi request reprint Comparative analysis of intracellular metabolites of Cephalosporium acremonium in pilot and industrial fermentation processes
    Yang Yang
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    Biotechnol Appl Biochem 59:228-37. 2012
    ..These findings provide insights into the intracellular metabolite characteristics and feasible regulation scheme to improve the titer of cephalosporin C in the industrial process...
  19. ncbi request reprint Oxidative burst in suspension culture of Taxus cuspidata induced by a laminar shear stress in short-term
    Rong Bin Han
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, P R China
    Biotechnol Prog 20:507-13. 2004
    ..A model is proposed to explain the oxidative burst in cultured T. cuspidata cells challenged with the shear stress...
  20. ncbi request reprint Signal role for activation of caspase-3-like protease and burst of superoxide anions during Ce4+-induced apoptosis of cultured Taxus cuspidata cells
    Zhi Qiang Ge
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Weijin Road 92, Nankai District, Tianjin 300072, P R China
    Biometals 18:221-32. 2005
    ..The burst of O2*- exerts its activity as an upstream of caspase-3-like activation. Our results also implicate that other signal pathways independent of an O2*- burst possibly participate in mediating caspase-3-like protease activation...
  21. doi request reprint Transcriptome analysis of differential responses of diploid and haploid yeast to ethanol stress
    Bing zhi Li
    Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    J Biotechnol 148:194-203. 2010
    ..Taken together, these results provide molecular insights into tolerance difference of haploid and diploid yeast strains and molecular information to further understand ethanol tolerance mechanism in yeast...
  22. doi request reprint Transcriptome shifts in response to furfural and acetic acid in Saccharomyces cerevisiae
    Bing zhi Li
    Key Laboratory of Systems Bioengineering Tianjin University, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin, 300072, People s Republic of China
    Appl Microbiol Biotechnol 86:1915-24. 2010
    ..This research has provided insights into molecular response to furfural and acetic acid in S. cerevisiae, and it will be helpful to construct more resistant strains for cellulosic ethanol production...
  23. ncbi request reprint Differential analysis of secondary metabolites by LC-MS following strain improvement of Streptomyces lydicus AS 4.2501
    Xiao Bing Li
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    Biotechnol Appl Biochem 45:107-18. 2006
    ....
  24. ncbi request reprint Inhibitor studies of isopentenyl pyrophosphate biosynthesis in suspension cultures of the yew Taxus chinensis var. mairei
    Yan Dong Wang
    P O Box 6888, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    Biotechnol Appl Biochem 37:39-43. 2003
    ..Furthermore, in combination with the results of previous studies, it is very probable that different IPP biosynthesis pathways exist during different growth phases in Taxus cells...
  25. ncbi request reprint Reactive oxygen species, cell growth, and taxol production of Taxus cuspidata cells immobilized on polyurethane foam
    De Ming Yin
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China
    Appl Biochem Biotechnol 127:173-85. 2005
    ..It is inferred that the intracellular and extracellular H2O2 inside the microenvironment might be one factor promoting taxol biosynthesis under the immobilization stress...
  26. ncbi request reprint Inhibition of rat vascular smooth muscle cell proliferation by extract of Ligusticum chuanxiong and Angelica sinensis
    Yong Zhong Hou
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    J Ethnopharmacol 100:140-4. 2005
    ..On the other hand, ELCAS significantly increased nitric oxide production of VSMC. The data suggest that ELCAS markedly inhibited VSMC proliferation by arresting G(1) to S progression, which may be associated with nitric oxide production...
  27. ncbi request reprint Ce(4+) induced down-regulation of ERK-like MAPK and activation of nucleases during the apoptosis of cultured Taxus cuspidata cells
    Zhi Qiang Ge
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    J Inorg Biochem 100:167-77. 2006
    ..cuspidata cells...
  28. doi request reprint Enhancement of 2-keto-gulonic acid yield by serial subcultivation of co-cultures of Bacillus cereus and Ketogulonicigenium vulgare
    Yang Zou
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, PR China
    Bioresour Technol 132:370-3. 2013
    ..We found that the serial subcultivation increased the yield of 2-KGA from 77% (original co-culture) to 93% (the 150th transfer of transferred co-culture). The resulted strains are of industrial interests for vitamin C production...
  29. pmc Integrated proteomic and metabolomic analysis of an artificial microbial community for two-step production of vitamin C
    Qian Ma
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    PLoS ONE 6:e26108. 2011
    ..megaterium provided key elements necessary for K. vulgare to grow better and produce more 2-KGA. The study represents the first attempt to decipher 2-KGA-producing microbial communities using quantitative systems biology analysis...
  30. doi request reprint Integrated investigation of lipidome and related signaling pathways uncovers molecular mechanisms of tetramethylpyrazine and butylidenephthalide protecting endothelial cells under oxidative stress
    Jie Yang
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, P R China
    Mol Biosyst 8:1789-97. 2012
    ..The current work suggests that the interaction of the lipidome and phospholipases can serve as a promising therapeutic target in oxidatively stressed ECs...
  31. doi request reprint Combinational expression of sorbose/sorbosone dehydrogenases and cofactor pyrroloquinoline quinone increases 2-keto-L-gulonic acid production in Ketogulonigenium vulgare-Bacillus cereus consortium
    Jin Du
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    Metab Eng 19:50-6. 2013
    ..vulgare (65.9±0.4gl(-1)) in shaking flasks. Our results demonstrated the balanced co-expression of both the key enzymes and the related cofactors was an efficient strategy to increase chemicals' biosynthesis. ..
  32. doi request reprint Comparison of the secondary metabolites in two scales of cephalosporin C (CPC) fermentation and two different post-treatment processes
    Ying Xiu Cao
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    J Ind Microbiol Biotechnol 40:95-103. 2013
    ..This indicated that the quality of the final product was more stable after the oxalic acid and formaldehyde were added in the post-treatment process. These findings provided new insight into industrial CPC production...
  33. ncbi request reprint Proteomic analysis reveals the spatial heterogeneity of immobilized Taxus cuspidata cells in support matrices
    Jing Sheng Cheng
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, P R China
    Proteomics 6:2199-207. 2006
    ..The abundance of S-adenosylmethionine synthetase, which was identified as one of the differentially expressed proteins, was positively correlated to the cell division activity in the immobilized cell cultures...
  34. ncbi request reprint Functional analysis of type II thioesterase of Streptomyces lydicus AS 4.2501
    Feng Ming Yu
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, PO Box 6888, Tianjin University, Tianjin 300072, P R China
    Appl Biochem Biotechnol 135:145-58. 2006
    ..Furthermore, targeted gene manipulation based on PCR was a powerful tool for studying gene function and metabolomics, especially when little was known about the genomic sequence of streptomyces...
  35. ncbi request reprint Comparative lipidomics analysis of cellular development and apoptosis in two Taxus cell lines
    Song Yang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Biochim Biophys Acta 1771:600-12. 2007
    ..These results suggest that the alternation of membrane phospholipids may regulate apoptosis, triggering an increase in taxol production of T. chinensis var. mairei cells...
  36. ncbi request reprint Comparison of burst of reactive oxygen species and activation of caspase-3 in apoptosis of K562 and HL-60 cells induced by docetaxel
    Dong Xu Cao
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, P O Box 6888, Tianjin University, Weijing Road 92, Tianjin 300072, China
    Cancer Lett 214:103-13. 2004
    ..While in K562 cells, docetaxel induced apoptosis after G2/M accumulation through ROS-independent or partially dependent pathways...
  37. ncbi request reprint [Induced-effects by additional taxol in suspension cultures of Taxus cuspidata cells]
    Zhen Yi Ma
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    Sheng Wu Gong Cheng Xue Bao 18:204-7. 2002
    ....
  38. ncbi request reprint A modified PCR system for amplifying beta-ketoacyl-ACP synthase gene fragments with DNA from Streptomyces luteogriseus
    Feng Ming Yu
    Department of Pharmaceutical Engineering, Tianjin University, P O Box 6888, 300072, Tianjin, PR China
    Biotechnol Lett 27:1277-82. 2005
    ..10% v/v) and dNTP (500 microM vs. 50-200 microM) and a lower annealing temperature (55 degrees C vs. 60-70 degrees C) than the normal PCR method used to amplify high GC content DNA...
  39. ncbi request reprint Ferulic acid inhibits vascular smooth muscle cell proliferation induced by angiotensin II
    Yong Zhong Hou
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Weijing Road 92, P O Box 6888, Tianjin 300072, PR China
    Eur J Pharmacol 499:85-90. 2004
    ..The inactivation of MAPKs and modulation of cell cycle proteins of ferulic acid may be of importance in preventing cardiovascular disease...
  40. doi request reprint Lipidomic analysis of apoptotic hela cells induced by Paclitaxel
    Xia Li
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, People s Republic of China
    OMICS 15:655-64. 2011
    ..Our study also demonstrates that lipidomics provide a powerful tool for better understanding anticancer mechanisms of chemotherapeutic agents and for biomarker screening...
  41. doi request reprint Metabolome analysis of differential responses of diploid and haploid yeast to ethanol stress
    Ming Zhu Ding
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    OMICS 14:553-61. 2010
    ..These findings provided underlying basis for better understanding diploid and haploid yeasts, and further breeding tolerant strains for efficient ethanol fermentation...
  42. doi request reprint Comparative lipidomic profiling of xylose-metabolizing S. cerevisiae and its parental strain in different media reveals correlations between membrane lipids and fermentation capacity
    Jinmei Xia
    PO Box 6888, Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin University, Tianjin 300072, PR China
    Biotechnol Bioeng 108:12-21. 2011
    ..Interestingly, PI synthesis lagged behind when available nutrients were limited, and PI levels were closely correlated with xylose metabolism...
  43. doi request reprint Analysis of phospholipids, sterols, and fatty acids in Taxus chinensis var. mairei cells in response to shear stress
    Pei pei Han
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    Biotechnol Appl Biochem 54:105-12. 2009
    ..These findings suggested alterations in membrane composition and hinted at a possible molecular basis for the mechanotransduction processes induced by shear stress in T. chinensis var. mairei cells...
  44. doi request reprint Dual effect of soluble materials in pretreated lignocellulose on simultaneous saccharification and co-fermentation process for the bioethanol production
    Lei Qin
    Key Laboratory of Systems Bioengineering Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Weijin Road 92, Nankai District, Tianjin 300072, PR China SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering Tianjin, Tianjin University, Weijin Road 92, Nankai District, Tianjin 300072, PR China Electronic address
    Bioresour Technol . 2016
    ..The dual effects of soluble materials on enzymatic hydrolysis and fermentation resulted in the reduction of ethanol yield as soluble materials increased in SSCF...
  45. pmc Inhibition of lignin-derived phenolic compounds to cellulase
    Lei Qin
    Key Laboratory of Systems Bioengineering Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Weijin Road 92, Nankai District, Tianjin, 300072 People s Republic of China SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering Tianjin, Tianjin University, Weijin Road 92, Nankai District, Tianjin, 300072 People s Republic of China
    Biotechnol Biofuels 9:70. 2016
    ..We investigated inhibition of phenolics on cellulase during enzymatic hydrolysis using vanillin as one of the typical lignin-derived phenolics and Avicel as cellulose substrate...
  46. ncbi request reprint Aqueous extract of Salvia miltiorrhoza regulates adhesion molecule expression of tumor necrosis factor alpha-induced endothelial cells by blocking activation of nuclear factor kappaB
    Mei Ding
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, P R China
    J Cardiovasc Pharmacol 45:516-24. 2005
    ..It may imply one of the mechanisms by which ESM exerts its beneficial effect preventing the progress of atherosclerosis...
  47. pmc Phospholipid metabolism in an industry microalga Chlorella sorokiniana: the impact of inoculum sizes
    Shuhuan Lu
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    PLoS ONE 8:e70827. 2013
    ..sorokiniana, which may work through thylakoid membrane and photosynthetic pathway. Thus this study would provide more potential targets for metabolic engineering to improve biofuel production and productivity in microalgae...
  48. doi request reprint A novel approach to active compounds identification based on support vector regression model and mean impact value
    Jian Lan Jiang
    Key Laboratory of Systems Bioengineering, Ministry of Education, Tianjin Key Laboratory of Biological and Pharmaceutical Engineering, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, China
    Chem Biol Drug Des 81:650-7. 2013
    ..prior to bioassay-guided separation. The use of a support vector regression model combined with mean impact value analysis could provide an efficient and economical approach for drug discovery from natural products...
  49. ncbi request reprint Synthesis and evaluation of water-soluble paclitaxel prodrugs
    Xia Feng
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, PR China
    Bioorg Med Chem Lett 12:3301-3. 2002
    ..The prodrugs showed highly improved water solubility, enhanced in vitro cytotoxicity and in vivo antitumor activity compared with the native drug, paclitaxel...
  50. ncbi request reprint Spatio-temporal distributions of metal ions and Taxol of Taxus cuspidata cells immobilized on polyurethane foam
    De Ming Yin
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin, 300072, PR China
    Biotechnol Lett 28:29-32. 2006
    ..3 and 3.7 times higher, while the K+ content was 5.5 times lower. Thus higher intracellular Ca2+ and Mg2+ contents and lower intracellular K+ content may favor the Taxol biosynthesis in immobilized Taxus cuspidata...
  51. ncbi request reprint Nitric oxide mediates inactivation of glutathione S-transferase in suspension culture of Taxus cuspidata during shear stress
    Yan Wen Gong
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    J Biotechnol 123:185-92. 2006
    ..Treatment with reactive nitrogen species (RNS) also caused inactivation of GST in cells. The results indicate that NO plays a crucial role in GST inactivation in Taxus cuspidata cells under shear stress...
  52. ncbi request reprint Inhibition of tumor metastasis in vivo by combination of paclitaxel and hyaluronic acid
    Dian Shu Yin
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, People s Republic of China
    Cancer Lett 243:71-9. 2006
    ..These results demonstrated that the combination of paclitaxel and hyaluronic acid may be an effective way to inhibit tumor migration...
  53. ncbi request reprint Improved cell growth and Taxol production of suspension-cultured Taxus chinensis var. mairei in alternating and direct current magnetic fields
    Gui Min Shang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, 300072, PR China
    Biotechnol Lett 26:875-8. 2004
    ..4-fold and 1.2-fold of that without exposure to a magnetic field...
  54. ncbi request reprint Comparison of JNK (c-Jun N-terminal kinase)-like MAPK (mitogen-activated protein kinase) phosphorylation between immobilized cultures and Couette-type shear reactor cultures of Taxus cuspidata (Japanese yew) cells
    Jing Sheng Cheng
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, People s Republic of China
    Biotechnol Appl Biochem 44:109-17. 2006
    ..cuspidata cells, these findings suggested that the JNK-like MAPK signal pathways may be involved in T. cuspidata cell response to the hydrodynamic shear stress, rather than to the p38-like MAPK...
  55. doi request reprint Reducing sugar loss in enzymatic hydrolysis of ethylenediamine pretreated corn stover
    Wen Chao Li
    Key Laboratory of Systems Bioengineering Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering Tianjin, Tianjin University, Tianjin 300072, PR China
    Bioresour Technol . 2016
    ..By decreasing temperature to 30°C, the loss of xylose yield reduced to only 3.8%, 3.6% and 4.2% with 7.5mL/L EDA in the enzymatic hydrolysis of AFEX pretreated CS, washed EDA pretreated CS and CS cellulose...
  56. doi request reprint In situ detoxification of dry dilute acid pretreated corn stover by co-culture of xylose-utilizing and inhibitor-tolerant Saccharomyces cerevisiae increases ethanol production
    Jia qing Zhu
    Key Laboratory of Systems Bioengineering Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China Center of Synthetic Biology, Collaborative Innovation Center of Chemical Science and Engineering Tianjin, Tianjin University, Tianjin 300072, PR China
    Bioresour Technol 218:380-7. 2016
    ..2% and 41.0% comparing to SSCF using individual inhibitor-tolerant and xylose-utilizing strain (increased from 48.5 and 41.7g/L to 58.8g/L), respectively, which suggest this co-culture system was very promising. ..
  57. doi request reprint Metabolic analysis reveals the amino acid responses of Streptomyces lydicus to pitching ratios during improving streptolydigin production
    Jing Sheng Cheng
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, People s Republic of China
    Appl Microbiol Biotechnol 97:5943-54. 2013
    ..Therefore, these findings provide new insights into the amino acid responses of S. lydicus E9 to pitching ratios and provide potential strategies to improve streptolydigin production...
  58. ncbi request reprint [Application of new method for data processing in metabonomic studies]
    Jing Li
    Department of Pharmaceutical Engineering, Institute of Chemical Engineering, Tianjin University, Tan 300072, China
    Yao Xue Xue Bao 41:47-53. 2006
    ..To search for and application of new method for data processing in metabonomic studies...
  59. ncbi request reprint Apoptotic cell death in suspension cultures of Taxus cuspidata co-treated with salicylic acid and hydrogen peroxide
    Jian Jun Qiao
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Biotechnol Lett 25:387-90. 2003
    ..These results show that exogenous salicylic acid and H2O2 could synergistically induce the apoptotic cell death of suspension cultures of Taxus cuspidata...
  60. ncbi request reprint A structured kinetic model for suspension cultures of Taxus chinensis var. mairei induced by an oligosaccharide from Fusarium oxysporum
    Chun Li
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P R China
    Biotechnol Lett 25:1335-43. 2003
    ....
  61. ncbi request reprint Biological responses of suspension cultures of Taxus chinensis var. mairei to shear stresses in the short term
    Zhong Dong Shi
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    Appl Biochem Biotechnol 110:61-74. 2003
    ..The results might shed light on a better understanding of the mechanism of plant cells responses to shear stress through the signal transduction pathway...
  62. ncbi request reprint Expression of arabinogalactan proteins involved in Taxol production by immobilized Taxus cuspidata cells
    Jing Sheng Cheng
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    J Biotechnol 133:96-102. 2008
    ..Taken together, these results implicate that the AGPs in T. cuspidata cells may be taken as potential signal molecular involved in regulating the Taxol production by immobilized T. cuspidata cells...
  63. doi request reprint Mass balance and transformation of corn stover by pretreatment with different dilute organic acids
    Lei Qin
    Key Laboratory of Systems Bioengineering, Ministry of Education Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    Bioresour Technol 112:319-26. 2012
    ..The maximum xylose yield was obtained after pretreatment with sulfuric and oxalic acid, but more xylooligomers were obtained after pretreatment with weaker acids...
  64. ncbi request reprint Characterization of the radical scavenging and antioxidant activities of danshensu and salvianolic acid B
    Guang Rong Zhao
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    Food Chem Toxicol 46:73-81. 2008
    ..Their radical scavenging and antioxidant properties might have potential applications in food and healthcare industry...
  65. pmc Metabolome profiling reveals metabolic cooperation between Bacillus megaterium and Ketogulonicigenium vulgare during induced swarm motility
    Jian Zhou
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, People s Republic of China
    Appl Environ Microbiol 77:7023-30. 2011
    ..This paper is the first to systematically identify a symbiotic interaction mechanism via metabolites in the ecosystem established by two isolated colonies of B. megaterium and K. vulgare...
  66. pmc Comparative proteomic analysis of experimental evolution of the Bacillus cereus-Ketogulonicigenium vulgare co-culture
    Qian Ma
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, Collaborative Innovation Center of Chemical Science and Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, P R China
    PLoS ONE 9:e91789. 2014
    ..cereus, together with the increased amino acids synthesis in evolved K. vulgare suggested that serial subcultivation result in enhanced synergistic cooperation between K. vulgare and B. cereus, enabling an increased production of 2-KGA. ..
  67. ncbi request reprint RGD-dependent mechanotransduction of suspension cultured Taxus cell in response to shear stress
    Hong Gao
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Biotechnol Prog 23:673-9. 2007
    ..Although the Arabidopsis genome database shows that the plant seems to lack a homologue of animal integrin, plant cells may use other RGD-binding proteins to recognize the RGD motif. The correlative mechanism is discussed...
  68. ncbi request reprint Salicylic acid-induced taxol production and isopentenyl pyrophosphate biosynthesis in suspension cultures of Taxus chinensis var. mairei
    Yan Dong Wang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    Cell Biol Int 31:1179-83. 2007
    ..581 mg/g dry wt) of the SA-treated system. It is thus inferred that the activated non-mevalonate pathway should be responsible for the formation of IPP in taxol biosynthesis in the presence of SA...
  69. doi request reprint Lipidomic analysis reveals activation of phospholipid signaling in mechanotransduction of Taxus cuspidata cells in response to shear stress
    Pei pei Han
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    FASEB J 23:623-30. 2009
    ..cuspidata cells. These results demonstrate that phospholipids and related phospholipases play important roles in mechanotransduction of T. cuspidata cells in response to shear stress...
  70. doi request reprint Inoculation-density-dependent responses and pathway shifts in Saccharomyces cerevisiae
    Jing Sheng Cheng
    Department of Pharmaceutical Engineering, Tianjin University, Tianjin, P R China
    Proteomics 9:4704-13. 2009
    ..These findings provide insights into yeast responses to inoculation size and optimizing the direct inoculation of active dry yeast fermentation, so as to improve the ethanol production...
  71. ncbi request reprint Inclusion complexes of paclitaxel and oligo(ethylenediamino) bridged bis(beta-cyclodextrin)s: solubilization and antitumor activity
    Yu Liu
    Department of Chemistry, State Key Laboratory of Elemento organic Chemistry, Nankai University, Tianjin 300071, China
    Bioorg Med Chem 12:5767-75. 2004
    ..This high antitumor activity, along with the satisfactory water solubility and high thermal stability of the 1/paclitaxel complex, will be potentially useful for its clinical application as a highly effective antitumor drug...
  72. ncbi request reprint Inclusion complexation and solubilization of paclitaxel by bridged bis(beta-cyclodextrin)s containing a tetraethylenepentaamino spacer
    Yu Liu
    Department of Chemistry, State Key Laboratory of Elemento organic Chemistry, Nankai University, Tianjin 300071, P R China
    J Med Chem 46:4634-7. 2003
    ..Furthermore, the cytotoxicity of the novel complexes was assessed using a K562 leukemia cell line which indicated that drug concentrations of 10 pg/mL elicited an inhibitory effect...
  73. doi request reprint Identification of antitumor constituents in curcuminoids from Curcuma longa L. based on the composition-activity relationship
    Jian Lan Jiang
    Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    J Pharm Biomed Anal 70:664-70. 2012
    ..These results support a method for the discovery of antitumor active constituents...
  74. doi request reprint Cerium elicitor-induced phosphatidic acid triggers apoptotic signaling development in Taxus cuspidata cell suspension cultures
    Song Yang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China
    Chem Phys Lipids 159:13-20. 2009
    ..After 2h of Ce(4+) elicitation, however, PA-induced O2(*-) burst, forming a negative regulation to MAPK activity, which in turn led to apoptotic signaling development...
  75. doi request reprint Process optimization to convert forage and sweet sorghum bagasse to ethanol based on ammonia fiber expansion (AFEX) pretreatment
    Bing zhi Li
    Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, PR China
    Bioresour Technol 101:1285-92. 2010
    ..The maximum final ethanol concentration in the fermentation broth was 30.9 g/L (forage sorghum) and 42.3 g/L (sweet sorghum bagasse). A complete mass balance for the process is given...
  76. doi request reprint Spatial-temporal distribution of nitric oxide involved in regulation of phenylalanine ammonialyase activation and Taxol production in immobilized Taxus cuspidata cells
    Wen Hai Xiao
    Department of Pharmaceutical Engineering, Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    J Biotechnol 139:222-8. 2009
    ..These results suggest that NO plays a signal role in regulation of PAL activity and Taxol production in immobilized T. cuspidata cells...
  77. doi request reprint Comparison of the secondary metabolites in Penicillium chrysogenum between pilot and industrial penicillin G fermentations
    Ying Xiu Cao
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, P O Box 6888, Tianjin 300072, People s Republic of China
    Appl Microbiol Biotechnol 89:1193-202. 2011
    ..These findings provided new insight into the changes and regulation of secondary metabolites in P. chrysogenum under different fermentation sizes...
  78. ncbi request reprint [Progress in detection methods of single nucleotide polymorphisms]
    Guang Rong Zhao
    Department of Pharmaceutical Engineering, College of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    Yi Chuan 27:123-9. 2005
    ..It could be expected that an accurate and sensitive, simple and easy-to-handle SNP technology with low cost and high throughput will be available on the basis of research breakthroughs of biochemistry, engineering and analytic software...
  79. doi request reprint Temporal quantitative proteomics of Saccharomyces cerevisiae in response to a nonlethal concentration of furfural
    Feng Ming Lin
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, PR China
    Proteomics 9:5471-83. 2009
    ..The expressions of 18 proteins related to stress response showed increased trends, including several highly induced heat shock proteins and proteins related to cellular signaling pathways...
  80. ncbi request reprint [Study on adsorption of danshen water-soluble compounds with macroporous resin D301]
    Zhi Ping Wang
    Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    Zhongguo Zhong Yao Za Zhi 29:854-7. 2004
    ..To study the adsorption and enrichment of effective compounds of danshensu, and protocatechualdehyde from Danshen water extracts by macroporous resin D301...
  81. pmc Balance of XYL1 and XYL2 expression in different yeast chassis for improved xylose fermentation
    Jian Zha
    Key Laboratory of Systems Bioengineering Tianjin University, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University Tianjin, P R China
    Front Microbiol 3:355. 2012
    ..The strain exhibited a 21.3% lower xylitol yield and a 40.0% higher ethanol yield. The results demonstrate the feasibility of the combinatorial strategy for construction of an efficient xylose-fermenting S. cerevisiae...
  82. doi request reprint Comprehensive quality evaluation of corn steep liquor in 2-keto-L-gulonic acid fermentation
    Yun Gao
    Key Laboratory of Systems Bioengineering, Tianjin University, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, P O Box 6888, Tianjin 300072, People s Republic of China
    J Agric Food Chem 59:9845-53. 2011
    ..The comprehensive approach provided an important platform to explore CSL marker compounds for quality evaluation in 2-KLG fermentation...
  83. pmc An environment-sensitive synthetic microbial ecosystem
    Bo Hu
    Key Laboratory of Systems Bioengineering, Ministry of Education and Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    PLoS ONE 5:e10619. 2010
    ..This synthetic ecosystem provides valuable information for addressing questions in ecology and may act as a chassis for construction of more complex microbial ecosystems...
  84. ncbi request reprint [Studies on paclitaxel-loaded nanoparticles of amphiphilic block copolymer]
    An Jie Dong
    School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
    Yao Xue Xue Bao 39:149-52. 2004
    ..To investigate the paclitaxel-loaded nanoparticles of poly(ethylene glycol)-b-poly(D,L-lactic acid) amphiphilic diblock copolymer (PMT)...
  85. ncbi request reprint Protective effect of Ligusticum chuanxiong and Angelica sinensis on endothelial cell damage induced by hydrogen peroxide
    Yong Zhong Hou
    P O Box 6888, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, 300072, PR China
    Life Sci 75:1775-86. 2004
    ..Our data also provide new evidence of Ligusticum chuanxiong and Angelica sinensis in preventing both cardiovascular and cerebrovascular diseases...
  86. doi request reprint Molecular responses of phospholipids of Taxus cuspidata (Japanese yew) to hydrodynamic shear stress in bubble columns
    Cheng Zhong
    Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, PO Box 6888, Tianjin 300072, People s Republic of China
    Biotechnol Appl Biochem 53:265-75. 2009
    ..These results suggest that PA accumulation plays an important role in the membrane degradation induced by hydrodynamic shear stress...
  87. doi request reprint Comparative lipidomics of four strains of Saccharomyces cerevisiae reveals different responses to furfural, phenol, and acetic acid
    Jin Mei Xia
    Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin, People s Republic of China
    J Agric Food Chem 57:99-108. 2009
    ..Further analysis of the distribution of different hydrocarbon chains revealed that both the saturation and the length of the chains helped in maintaining proper fluidity of membranes...
  88. doi request reprint fabC of Streptomyces lydicus involvement in the biosynthesis of streptolydigin
    Guang Rong Zhao
    Key Laboratory of Systems Bioengineering, Ministry of Education, Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, P O Box 6888, People s Republic of China
    Appl Microbiol Biotechnol 83:305-13. 2009
    ..Our results provide the first genetic and metabolic evidence that SlyfabCF is shared by fatty acid synthesis and antibiotic streptolydigin synthesis...