Michael P Walsh

Summary

Affiliation: University of Calgary
Country: Canada

Publications

  1. pmc The role of actin filament dynamics in the myogenic response of cerebral resistance arteries
    Michael P Walsh
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Cereb Blood Flow Metab 33:1-12. 2013
  2. doi request reprint Vascular smooth muscle myosin light chain diphosphorylation: mechanism, function, and pathological implications
    Michael P Walsh
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    IUBMB Life 63:987-1000. 2011
  3. doi request reprint Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation
    Michael P Walsh
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    Am J Physiol Renal Physiol 300:F73-85. 2011
  4. pmc The force is with alpha-actinin: dynamic regulation of the extracellular matrix-cytoskeletal connection in airway smooth muscle
    Michael P Walsh
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    J Physiol 572:611-2. 2006
  5. pmc Ser-2030, but not Ser-2808, is the major phosphorylation site in cardiac ryanodine receptors responding to protein kinase A activation upon beta-adrenergic stimulation in normal and failing hearts
    Bailong Xiao
    Cardiovascular Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada
    Biochem J 396:7-16. 2006
  6. doi request reprint Ca2+-independent contraction of longitudinal ileal smooth muscle is potentiated by a zipper-interacting protein kinase pseudosubstrate peptide
    Eikichi Ihara
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Faculty of Medicine, Calgary, Alberta, Canada
    Am J Physiol Gastrointest Liver Physiol 297:G361-70. 2009
  7. doi request reprint Prostate-apoptosis response-4 phosphorylation in vascular smooth muscle
    Justin A MacDonald
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, 3280 Hospital Drive NW, Calgary, AB, Canada T2N 4Z6
    Arch Biochem Biophys 535:84-90. 2013
  8. pmc Pressure-dependent contribution of Rho kinase-mediated calcium sensitization in serotonin-evoked vasoconstriction of rat cerebral arteries
    Ahmed F El-Yazbi
    The Smooth Muscle Research Group, Department of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    J Physiol 588:1747-62. 2010
  9. pmc Ca2+ sensitization via phosphorylation of myosin phosphatase targeting subunit at threonine-855 by Rho kinase contributes to the arterial myogenic response
    Rosalyn P Johnson
    Department of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Physiol 587:2537-53. 2009
  10. doi request reprint Atorvastatin inhibits myocardin expression in vascular smooth muscle cells
    Jingjing Li
    Department of Biochemistry and Molecular Biology, The Libin Cardiovascular Institute of Alberta, The University of Calgary, Health Sciences Center, 3330 Hospital Dr NW, Calgary, Alberta, Canada T2N 4N1
    Hypertension 60:145-53. 2012

Collaborators

Detail Information

Publications70

  1. pmc The role of actin filament dynamics in the myogenic response of cerebral resistance arteries
    Michael P Walsh
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Cereb Blood Flow Metab 33:1-12. 2013
    ..This review describes these three mechanisms, emphasizing recent developments regarding the importance of dynamic actin polymerization in the myogenic response of the cerebral vasculature...
  2. doi request reprint Vascular smooth muscle myosin light chain diphosphorylation: mechanism, function, and pathological implications
    Michael P Walsh
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    IUBMB Life 63:987-1000. 2011
    ..Kinases such as ILK, ROCK, and ZIPK, therefore, are potential therapeutic targets in the treatment of, for example, cerebral vasospasm following subarachnoid hemorrhage and atherosclerosis...
  3. doi request reprint Rho-associated kinase plays a role in rabbit urethral smooth muscle contraction, but not via enhanced myosin light chain phosphorylation
    Michael P Walsh
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    Am J Physiol Renal Physiol 300:F73-85. 2011
    ..We conclude that ROK plays an important role in urethral smooth muscle contraction, but not via inhibition of MLCP or polymerization of actin...
  4. pmc The force is with alpha-actinin: dynamic regulation of the extracellular matrix-cytoskeletal connection in airway smooth muscle
    Michael P Walsh
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada T2N 4N1
    J Physiol 572:611-2. 2006
  5. pmc Ser-2030, but not Ser-2808, is the major phosphorylation site in cardiac ryanodine receptors responding to protein kinase A activation upon beta-adrenergic stimulation in normal and failing hearts
    Bailong Xiao
    Cardiovascular Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada
    Biochem J 396:7-16. 2006
    ..Our results also suggest that phosphorylation of RyR2 at Ser-2030 may be an important event associated with altered Ca2+ handling and cardiac arrhythmia that is commonly observed in heart failure upon beta-adrenergic stimulation...
  6. doi request reprint Ca2+-independent contraction of longitudinal ileal smooth muscle is potentiated by a zipper-interacting protein kinase pseudosubstrate peptide
    Eikichi Ihara
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Faculty of Medicine, Calgary, Alberta, Canada
    Am J Physiol Gastrointest Liver Physiol 297:G361-70. 2009
    ..AV25 or molecules based on its structure could be used in therapeutic situations to induce contractility in diseases of the gastrointestinal tract associated with hypomotility...
  7. doi request reprint Prostate-apoptosis response-4 phosphorylation in vascular smooth muscle
    Justin A MacDonald
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, 3280 Hospital Drive NW, Calgary, AB, Canada T2N 4Z6
    Arch Biochem Biophys 535:84-90. 2013
    ..We conclude that phosphorylation of Par-4 does not regulate contraction of this vascular smooth muscle tissue by inducing dissociation of Par-4 from MYPT1 to allow phosphorylation of MYPT1 and inhibition of MLCP...
  8. pmc Pressure-dependent contribution of Rho kinase-mediated calcium sensitization in serotonin-evoked vasoconstriction of rat cerebral arteries
    Ahmed F El-Yazbi
    The Smooth Muscle Research Group, Department of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    J Physiol 588:1747-62. 2010
    ..These findings provide novel evidence of an interplay between myogenic- and agonist-induced vasoconstriction in cerebral resistance arteries...
  9. pmc Ca2+ sensitization via phosphorylation of myosin phosphatase targeting subunit at threonine-855 by Rho kinase contributes to the arterial myogenic response
    Rosalyn P Johnson
    Department of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Physiol 587:2537-53. 2009
    ..In contrast, suppression of the myogenic response by PKC inhibitors cannot be attributed to block of Ca(2+) sensitization mediated by CPI-17 or MYPT1 phosphorylation...
  10. doi request reprint Atorvastatin inhibits myocardin expression in vascular smooth muscle cells
    Jingjing Li
    Department of Biochemistry and Molecular Biology, The Libin Cardiovascular Institute of Alberta, The University of Calgary, Health Sciences Center, 3330 Hospital Dr NW, Calgary, Alberta, Canada T2N 4N1
    Hypertension 60:145-53. 2012
    ..We conclude that ATV inhibits myocardin gene expression in vivo and in vitro, suggesting a novel mechanism for ATV inhibition of vascular contraction...
  11. ncbi request reprint Inhibition of zipper-interacting protein kinase function in smooth muscle by a myosin light chain kinase pseudosubstrate peptide
    Eikichi Ihara
    Smooth Muscle Research Group and Dept of Biochemistry and Molecular Biology, University of Calgary, Faculty of Medicine, 3330 Hospital Dr NW, Calgary, Alberta T2N 4N1, Canada
    Am J Physiol Cell Physiol 292:C1951-9. 2007
    ..In summary, we present the SM1 peptide as a novel inhibitor of ZIPK. We also conclude that the SM1 peptide, which has no effect on ILK, can be used to distinguish between ZIPK and ILK effects in smooth muscle tissues...
  12. ncbi request reprint Characterization of protein kinase pathways responsible for Ca2+ sensitization in rat ileal longitudinal smooth muscle
    Eikichi Ihara
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Faculty of Medicine, Calgary, Alberta, Canada
    Am J Physiol Gastrointest Liver Physiol 293:G699-710. 2007
    ..The negative regulation of MLCP by PKC and MAPKs during microcystin-induced contraction at pCa 9, which is not observed in vascular smooth muscle, may be unique to phasic smooth muscle...
  13. pmc Ca2+ sensitization due to myosin light chain phosphatase inhibition and cytoskeletal reorganization in the myogenic response of skeletal muscle resistance arteries
    Alejandro Moreno-Domínguez
    The Smooth Muscle Research Group, Department of Physiology and Pharmacology, Libin Cardiovascular Institute and Hotchkiss Brain Institute, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    J Physiol 591:1235-50. 2013
    ....
  14. pmc Thromboxane A2-induced contraction of rat caudal arterial smooth muscle involves activation of Ca2+ entry and Ca2+ sensitization: Rho-associated kinase-mediated phosphorylation of MYPT1 at Thr-855, but not Thr-697
    David P Wilson
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    Biochem J 389:763-74. 2005
    ....
  15. doi request reprint Inhibition of rat aortic smooth muscle contraction by 2-methoxyestradiol
    Yu Gui
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, 3330 Hospital Dr NW, Calgary, Alberta, T2N 4N1 Canada
    Am J Physiol Heart Circ Physiol 295:H1935-42. 2008
    ..2-ME does not mediate the inhibitory effect of E2 on smooth muscle contraction. These results support a potentially important role of 2-ME in the regulation of smooth muscle tone in the vasculature...
  16. ncbi request reprint Phosphorylation of the protein phosphatase type 1 inhibitor protein CPI-17 by protein kinase C
    Michael P Walsh
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada
    Methods Mol Biol 365:209-23. 2007
    ..Physiologically, this mechanism can account for some instances of Ca2+ sensitization of smooth muscle contraction (i.e., an increase in force in response to agonist stimulation without a change in [Ca2+]i)...
  17. pmc Chemical genetics of zipper-interacting protein kinase reveal myosin light chain as a bona fide substrate in permeabilized arterial smooth muscle
    Lori D Moffat
    Smooth Muscle Research Group and the Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta T2N 4Z6, Canada
    J Biol Chem 286:36978-91. 2011
    ..LC(20) was thereby confirmed as a direct target of ZIPK; however, no phosphorylation of MYPT1 was detected. We conclude that ZIPK is involved in the regulation of smooth muscle contraction through direct phosphorylation of LC(20)...
  18. pmc Rho-kinase-mediated suppression of KDR current in cerebral arteries requires an intact actin cytoskeleton
    Kevin D Luykenaar
    Department of Physiology, University of Calgary, Alberta, Canada
    Am J Physiol Heart Circ Physiol 296:H917-26. 2009
    ..We propose that UTP initiates Rho-kinase-mediated remodeling of the actin cytoskeleton and consequently suppresses the K(DR) current, thereby facilitating the depolarization and constriction of cerebral arteries...
  19. pmc Cross-talk between Rho-associated kinase and cyclic nucleotide-dependent kinase signaling pathways in the regulation of smooth muscle myosin light chain phosphatase
    Michael E Grassie
    Smooth Muscle Research Group and Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, Alberta T2N 4Z6, Canada
    J Biol Chem 287:36356-69. 2012
    ....
  20. ncbi request reprint Characterization of a novel PKA phosphorylation site, serine-2030, reveals no PKA hyperphosphorylation of the cardiac ryanodine receptor in canine heart failure
    Bailong Xiao
    Cardiovascular Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, AB, Canada
    Circ Res 96:847-55. 2005
    ..Together, our data indicate that serine-2030 is a major PKA phosphorylation site in RyR2 responding to acute beta-adrenergic stimulation, and that RyR2 is not hyperphosphorylated by PKA in canine HF...
  21. ncbi request reprint Functional consequence of protein kinase A-dependent phosphorylation of the cardiac ryanodine receptor: sensitization of store overload-induced Ca2+ release
    Bailong Xiao
    Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 282:30256-64. 2007
    ..Collectively, our data indicate that PKA-dependent phosphorylation enhances the response of RyR2 to luminal Ca(2+) and reduces the threshold for SOICR and that this effect of PKA is largely mediated by phosphorylation at Ser-2,030...
  22. doi request reprint A highly sensitive technique to measure myosin regulatory light chain phosphorylation: the first quantification in renal arterioles
    Kosuke Takeya
    Department of Pharmacology, Univ of Calgary Faculty of Medicine, Smooth Muscle Research Group, 3330 Hospital Dr N W, Calgary, Alberta T2N 4N1, Canada
    Am J Physiol Renal Physiol 294:F1487-92. 2008
    ....
  23. pmc Identification and functional characterization of protein kinase A-catalyzed phosphorylation of potassium channel Kv1.2 at serine 449
    Rosalyn P Johnson
    Smooth Muscle Research Group, Southern Alberta Mass Spectrometry Centre, University of Calgary, Calgary, Alberta, Canada
    J Biol Chem 284:16562-74. 2009
    ..2-S449D or Kv1.2-S440A/S441A/S449A. We conclude that Ser-449 in Kv1.2 is a site of PKA phosphorylation and a potential molecular mechanism for Kv1-containing KDR channel modulation by agonists via PKA activation...
  24. doi request reprint Uridine adenosine tetraphosphate induces contraction of airway smooth muscle
    Yu Gui
    Dept of Physiology and Pharmacology, Faculty of Medicine, Univ of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Am J Physiol Lung Cell Mol Physiol 301:L789-94. 2011
    ..This study will add to our understanding of the pathophysiological roles of extracellular nucleotides in the lung...
  25. pmc Integrin-linked kinase is responsible for Ca2+-independent myosin diphosphorylation and contraction of vascular smooth muscle
    David P Wilson
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    Biochem J 392:641-8. 2005
    ..ILK is probably the kinase responsible for myosin diphosphorylation in vascular smooth muscle cells and tissues...
  26. ncbi request reprint The role of RhoA and Rho-associated kinase in vascular smooth muscle contraction
    Karl Swärd
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, 3330 Hospital Drive NW, Calgary, Alberta, Canada T2N 4N1
    Curr Hypertens Rep 5:66-72. 2003
    ..Indeed, ROK inhibitors restore normal blood pressure in several rat hypertensive models...
  27. ncbi request reprint Identification of a novel interaction between the Ca(2+)-binding protein S100A11 and the Ca(2+)- and phospholipid-binding protein annexin A6
    Ning Chang
    Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, 3330 Hospital Dr NW, Calgary, Alberta, Canada T2N 4N1
    Am J Physiol Cell Physiol 292:C1417-30. 2007
    ....
  28. ncbi request reprint Enzymatic assays to compare calmodulin isoforms, mutants, and chimeras
    Michael P Walsh
    Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, AB, Canada
    Methods Mol Biol 173:339-54. 2002
  29. doi request reprint The GPER agonist G-1 induces mitotic arrest and apoptosis in human vascular smooth muscle cells independent of GPER
    Yu Gui
    The Smooth Muscle Research Group, Department of Physiology and Pharmacology, Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, Alberta, Canada
    J Cell Physiol 230:885-95. 2015
    ..We conclude that G-1 inhibits proliferation of SMCs through mechanisms involving mitotic arrest and apoptosis, independent of GPER and the MAPK pathway...
  30. pmc Structural analysis of a calmodulin variant from rice: the C-terminal extension of OsCaM61 regulates its calcium binding and enzyme activation properties
    Mostafa Jamshidiha
    From the Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada and
    J Biol Chem 288:32036-49. 2013
    ..Altogether, our data show that the tail is not simply a linker between the prenyl group and the protein but that it also provides a new regulatory mechanism that some plants have developed to fine-tune Ca(2+) signaling events. ..
  31. doi request reprint Rapamycin inhibits hydrogen peroxide-induced loss of vascular contractility
    Ge Gao
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, Univ of Calgary, Calgary, Alberta, Canada
    Am J Physiol Heart Circ Physiol 300:H1583-94. 2011
    ..We conclude that rapamycin inhibits H₂O₂-induced loss of vascular contractility, likely through an mTORC2-calcineurin pathway...
  32. ncbi request reprint Identification of the linker histone H1 as a protein kinase Cepsilon-binding protein in vascular smooth muscle
    Mingcai Zhao
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, 330 Hospital Drive N W, Calgary, AB T2N 4N1, Canada
    Biochem Cell Biol 82:538-46. 2004
    ..Since PKC isoenzymes have been implicated in regulation of gene expression, stable interaction with histone H1 may be an important step in this process...
  33. pmc Stromatoxin-sensitive, heteromultimeric Kv2.1/Kv9.3 channels contribute to myogenic control of cerebral arterial diameter
    Xi Zoë Zhong
    The Smooth Muscle Research Group, Department of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, 3330 Hospital Drive N W, Calgary, Alberta, Canada T2N 4N1
    J Physiol 588:4519-37. 2010
    ....
  34. ncbi request reprint Abnormal myosin phosphatase targeting subunit 1 phosphorylation and actin polymerization contribute to impaired myogenic regulation of cerebral arterial diameter in the type 2 diabetic Goto-Kakizaki rat
    Khaled S Abd-Elrahman
    The Smooth Muscle Research Group, Departments of Physiology and Pharmacology, Libin Cardiovascular Institute and Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Alberta, Canada
    J Cereb Blood Flow Metab 37:227-240. 2017
    ..We conclude that abnormal Rho-associated kinase-mediated Ca2+ sensitization contributes to the dysfunctional cerebral myogenic response in the Goto-Kakizaki model of type 2 diabetes...
  35. ncbi request reprint The functional consequence of RhoA knockdown by RNA interference in rat cerebral arteries
    Randolph L Corteling
    Department of Physiology and Biophysics, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta, Canada
    Am J Physiol Heart Circ Physiol 293:H440-7. 2007
    ..It also demonstrates that the principal role of RhoA in agonist-induced constriction is to facilitate the formation of F-actin, the physical structure to which phosphorylated myosin binds to elicit arterial constriction...
  36. pmc Phosphorylation of the myosin phosphatase inhibitors, CPI-17 and PHI-1, by integrin-linked kinase
    Jing Ti Deng
    Smooth Muscle Research Group and Canadian Institutes of Health Research Group in Regulation of Vascular Contractility, University of Calgary Faculty of Medicine, 3330 Hospital Drive N W, Calgary, Alberta T2N 4N1, Canada
    Biochem J 367:517-24. 2002
    ..We conclude that ILK may activate smooth-muscle contraction both directly, via phosphorylation of myosin, and indirectly, via phosphorylation and activation of CPI-17 and PHI-1, leading to inhibition of MLCP...
  37. ncbi request reprint Protein kinase A phosphorylation at serine-2808 of the cardiac Ca2+-release channel (ryanodine receptor) does not dissociate 12.6-kDa FK506-binding protein (FKBP12.6)
    Bailong Xiao
    Cardiovascular Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada
    Circ Res 94:487-95. 2004
    ..6 from or prevent FKBP12.6 from binding to RyR2. Taken together, our results do not support the notion that PKA phosphorylation at serine-2808 dissociates FKBP12.6 from RyR2...
  38. ncbi request reprint Key role of Kv1 channels in vasoregulation
    Tim T Chen
    The Smooth Muscle Research Group, Faculty of Medicine, University of Calgary, Alberta, Canada
    Circ Res 99:53-60. 2006
    ....
  39. doi request reprint A Small Molecule Pyrazolo[3,4-d]Pyrimidinone Inhibitor of Zipper-Interacting Protein Kinase Suppresses Calcium Sensitization of Vascular Smooth Muscle
    Justin A MacDonald
    Department of Biochemistry and Molecular Biology, University of Calgary, Cumming School of Medicine, Calgary, Alberta, Canada J A M, C S, S B, A A G, M P W Department of Experimental Medical Science, Lund University, Lund, Sweden K S and Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina D A C, T A J H
    Mol Pharmacol 89:105-17. 2016
    ..These effects of HS38 suggest that ZIPK also lies downstream from G protein-coupled receptors that signal through both Gα12/13 and Gαq/11...
  40. doi request reprint α5-Integrin-mediated cellular signaling contributes to the myogenic response of cerebral resistance arteries
    Olaia Colinas
    Smooth Muscle Research Group, Department of Physiology and Pharmacology, Hotchkiss Brain Institute and Libin Cardiovascular Institute, University of Calgary, Alberta, Canada Electronic address
    Biochem Pharmacol 97:281-91. 2015
    ..We conclude that mechanotransduction by integrin adhesions and subsequent cellular signaling play a fundamental role in the cerebral arterial myogenic response. ..
  41. doi request reprint Potential Role of Glycogen Synthase Kinase-3β in Regulation of Myocardin Activity in Human Vascular Smooth Muscle Cells
    Yi Xia Zhou
    Department of Biochemistry and Molecular Biology, Smooth Muscle Research Group, Libin Cardiovascular Institute of Alberta, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Cell Physiol 231:393-402. 2016
    ..In summary, our results indicate that inhibition of GSK-3β reduces myocardin transcriptional activity, suggesting a role for GSK-3β in myocardin transcriptional activity and smooth muscle differentiation...
  42. doi request reprint Endothelin-1, but not angiotensin II, induces afferent arteriolar myosin diphosphorylation as a potential contributor to prolonged vasoconstriction
    Kosuke Takeya
    1 Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada 2 Smooth Muscle Research Group and Department of Physiology and Pharmacology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    Kidney Int 87:370-81. 2015
    ..Endothelin-1, but not angiotensin II, induces phosphorylation of LC20 at Thr18. This could contribute to the prolonged vasoconstrictor response to endothelin-1. ..
  43. ncbi request reprint Myosin heavy chain expression in renal afferent and efferent arterioles: relationship to contractile kinetics and function
    Mitsuya Shiraishi
    Smooth Muscle Research Group, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    FASEB J 17:2284-6. 2003
    ..Our findings thus point to the renal microcirculation as a unique and important example of smooth muscle adaptation in regard to MHC isoform expression and physiological function...
  44. pmc Myosin regulatory light chain diphosphorylation slows relaxation of arterial smooth muscle
    Cindy Sutherland
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 287:24064-76. 2012
    ....
  45. doi request reprint Signaling mechanisms mediating uridine adenosine tetraphosphate-induced proliferation of human vascular smooth muscle cells
    Yu Gui
    Smooth Muscle Research Group, Department of Physiology and Pharmacology, University of Calgary, Calgary, Alberta, Canada
    J Cardiovasc Pharmacol 58:654-62. 2011
    ..This work stresses the concept that dinucleotides, like mononucleotides, play potentially important roles in the regulation of vascular function...
  46. ncbi request reprint Activation of smooth muscle myosin light chain kinase by calmodulin. Role of LYS(30) and GLY(40)
    Jacquelyn E Van Lierop
    Smooth Muscle Research Group and the Canadian Institutes of Health Research Group in Regulation of Vascular Contractility, Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 277:6550-8. 2002
    ..e., activation of MLCK, or this domain may be required to stabilize the conformation of (Ca(2+))(4)-CaM necessary for MLCK activation...
  47. doi request reprint The myosin phosphatase targeting protein (MYPT) family: a regulated mechanism for achieving substrate specificity of the catalytic subunit of protein phosphatase type 1δ
    Michael E Grassie
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, AB, Canada
    Arch Biochem Biophys 510:147-59. 2011
    ..With such a wide range of regulatory targets, MYPT family members have been implicated in diverse pathological events, including hypertension, Parkinson's disease and cancer...
  48. doi request reprint Do TRPC-like currents and G protein-coupled receptors interact to facilitate myogenic tone development?
    Yana Anfinogenova
    Department of Physiology and Pharmacology, Hotchkiss Brain and Libin Cardiovascular Institutes, University of Calgary, Alberta, Canada
    Am J Physiol Heart Circ Physiol 301:H1378-88. 2011
    ..In summary, this study found no evidence that G(q/11)-coupled receptor activation augments the mechanosensitivity of a TRPC-like current and consequently the myogenic responsiveness of anterior cerebral arteries...
  49. pmc A novel inhibitory effect of oxazol-5-one compounds on ROCKII signaling in human coronary artery vascular smooth muscle cells
    Abdulhameed Al-Ghabkari
    Department of Biochemistry and Molecular Biology, University of Calgary, 3280 Hospital Drive NW, Calgary, AB, T2N 4Z6, Canada
    Sci Rep 6:32118. 2016
    ..In addition, CASMCs treated with DI underwent cytoskeletal changes that were associated with diminution of cofilin phosphorylation. We conclude that DI is not selective for ZIPK and is a potent inhibitor of ROCKII. ..
  50. ncbi request reprint Predisposition to tetraploidy in pulmonary vascular smooth muscle cells derived from the Eker rats
    Yu Gui
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada
    Am J Physiol Lung Cell Mol Physiol 293:L702-11. 2007
    ..Therefore, our results demonstrate that Tsc2(+/EK) SMCs are predisposed to undergo tetraploidization, involving activation of the mTOR pathway. These findings suggest an important role of Tsc2 in regulation of the cell cycle...
  51. doi request reprint Up4A stimulates endothelium-independent contraction of isolated rat pulmonary artery
    Yu Gui
    Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    Am J Physiol Lung Cell Mol Physiol 294:L733-8. 2008
    ..Up(4)A, therefore, potentially plays an important role in the regulation of pulmonary vascular tone...
  52. doi request reprint Matrix metalloproteinase-2 proteolysis of calponin-1 contributes to vascular hypocontractility in endotoxemic rats
    Michele M Castro
    Department of Pharmacology, Cardiovascular Research Centre, Mazankowski Alberta Heart Institute, 4 62 Heritage Medical Research Centre, University of Alberta, Edmonton, Alberta, T6G 2S2, Canada
    Arterioscler Thromb Vasc Biol 32:662-8. 2012
    ..We hypothesized that calponin-1 may be proteolyzed by MMP-2 in endotoxemia-induced vascular hypocontractility...
  53. pmc Protein kinase C modulation of recombinant ATP-sensitive K(+) channels composed of Kir6.1 and/or Kir6.2 expressed with SUR2B
    Kevin S Thorneloe
    Smooth Muscle Research Group and Canadian Institutes of Health Research Group in Regulation of Vascular Contractility, University of Calgary, Calgary, Alberta, Canada
    J Physiol 541:65-80. 2002
    ..1 and their associated SUR2B subunits...
  54. ncbi request reprint Localization of telokin at the intercalated discs of cardiac myocytes
    Gary J Kargacin
    Department of Physiology and Biophysics, University of Calgary, Calgary, Alta, Canada T2N 4N1
    Arch Biochem Biophys 456:151-60. 2006
    ....
  55. ncbi request reprint A highly sensitive method for quantification of myosin light chain phosphorylation by capillary isoelectric focusing with laser-induced fluorescence detection
    Mitsuya Shiraishi
    Smooth Muscle Research Group, University of Calgary, Faculty of Medicine, Calgary, Alberta, Canada
    Electrophoresis 26:571-80. 2005
    ..This method is therefore applicable to quantitative analysis of phosphorylation of minute quantities of LC20...
  56. doi request reprint Analysis of phosphorylation of the myosin-targeting subunit of myosin light chain phosphatase by Phos-tag SDS-PAGE
    Cindy Sutherland
    Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada
    Am J Physiol Cell Physiol 310:C681-91. 2016
    ..Phos-tag SDS-PAGE thus provides a suitable and convenient method for analysis of the complex, multisite MYPT1 phosphorylation events involved in the regulation of myosin light chain phosphatase activity and smooth muscle contraction. ..
  57. doi request reprint A role for the Ca(2+)-dependent tyrosine kinase Pyk2 in tonic depolarization-induced vascular smooth muscle contraction
    Ryan D Mills
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive N W, Calgary, AB, T2N 4N1, Canada
    J Muscle Res Cell Motil 36:479-89. 2015
    ..Investigations into the mechanism of activation of RhoA by Ca(2+) have implicated a genistein-sensitive tyrosine kinase, and recent evidence indicates this to be the Ca(2+)-dependent tyrosine kinase, Pyk2. ..
  58. pmc The effects of knockdown of rho-associated kinase 1 and zipper-interacting protein kinase on gene expression and function in cultured human arterial smooth muscle cells
    Jing Ti Deng
    Smooth Muscle Research Group and Department of Biochemistry and Molecular Biology, University of Calgary, Alberta, Canada
    PLoS ONE 10:e0116969. 2015
    ..We conclude that ROCK1 and ZIPK have diverse, but predominantly distinct regulatory functions in vascular SMC and that ROCK1-mediated activation of ZIPK is not involved in most of these functions. ..
  59. doi request reprint Abnormal Rho-associated kinase activity contributes to the dysfunctional myogenic response of cerebral arteries in type 2 diabetes
    Khaled S Abd-Elrahman
    The Smooth Muscle Research Group, Libin Cardiovascular Institute, Hotchkiss Brain Institute, and the Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB T2N 4N1, Canada
    Can J Physiol Pharmacol 93:177-84. 2015
    ..Here, we review recent advances in our understanding of the role of inappropriate Rho-associated kinase activity as a cause of impaired myogenic regulation of cerebral arterial diameter in type 2 diabetes. ..
  60. pmc A role for the tyrosine kinase Pyk2 in depolarization-induced contraction of vascular smooth muscle
    Ryan D Mills
    From the Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, Libin Cardiovascular Institute and Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta T2N 4N1, Canada and
    J Biol Chem 290:8677-92. 2015
    ..The resulting sustained elevation of LC20 phosphorylation then accounts for the tonic contractile response to membrane depolarization. ..
  61. ncbi request reprint Endoreduplication of human smooth muscle cells induced by 2-methoxyestradiol: a role for cyclin-dependent kinase 2
    Yu Gui
    Smooth Muscle Research Group, Department of Biochemistry and Molecular Biology, Faculty of Medicine, Univ of Calgary, Calgary, Alberta, Canada
    Am J Physiol Heart Circ Physiol 292:H1313-20. 2007
    ..We conclude that 2-ME induces endoreduplication of human SMCs and Cdk2 plays an important role in endoreduplication in response to 2-ME...
  62. ncbi request reprint Ca2+ activation of smooth muscle contraction: evidence for the involvement of calmodulin that is bound to the triton insoluble fraction even in the absence of Ca2+
    David P Wilson
    Department of Biochemistry and Molecular Biology, University of Calgary Faculty of Medicine, Calgary, Alberta T2N 4N1, Canada
    J Biol Chem 277:2186-92. 2002
    ..Immobilized CaM, therefore, resembles troponin C, the Ca(2+)-binding regulatory protein of striated muscle, which is also bound to the thin filament in a Ca(2+)-independent manner...
  63. ncbi request reprint Structures and metal-ion-binding properties of the Ca2+-binding helix-loop-helix EF-hand motifs
    Jessica L Gifford
    Structural Biology Research Group, Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada T2N 1N4
    Biochem J 405:199-221. 2007
    ..A structural approach is used in this review to examine the diversity of family members, and a biophysical perspective provides insight into the ability of the EF-hand motif to bind Ca2+ with a wide range of affinities...
  64. ncbi request reprint Distinct kinases are involved in contraction of cat esophageal and lower esophageal sphincter smooth muscles
    Nayoung Kim
    Department of Medicine, Seoul National University, Bundang Hospital, Seoungnam, Gyeronggi Do 463 707, Korea
    Am J Physiol Cell Physiol 287:C384-94. 2004
    ..We conclude that phosphatase inhibition potentiates the effects of MLCK in LES but not in ESO. Contraction of ESO is mediated by activation of PKCepsilon, MEK, ERK1/2, and ILK...
  65. ncbi request reprint Modulation of PGF2alpha- and hypoxia-induced contraction of rat intrapulmonary artery by p38 MAPK inhibition: a nitric oxide-dependent mechanism
    Greg A Knock
    Dept of Asthma, Allergy and Respiratory Science, School of Medicine, King s College London, London SE1 9RT, UK
    Am J Physiol Lung Cell Mol Physiol 289:L1039-48. 2005
    ..We conclude that p38 MAPK contributes to PGF(2alpha)- and hypoxia-induced constriction of rat IPA primarily by antagonizing the underlying Ca(2+)-desensitizing actions of NO...
  66. ncbi request reprint Augmentation of alpha1-adrenoceptor-mediated contraction by warming without increased phosphorylation of myosin in rat caudal arterial smooth muscle
    Mitsuo Mita
    Department of Pharmacodynamics, Meiji Pharmaceutical University, Tokyo, Japan
    J Pharmacol Sci 99:61-7. 2005
    ..58 +/- 0.08 mol P(i)/mol LC20 at room temperature and 0.49 +/- 0.05 mol P(i)/mol LC20 at 37 degrees C. We conclude, therefore, that the enhanced cirazoline-induced contraction at 37 degrees C is not due to increased LC20 phosphorylation...
  67. doi request reprint AMP-activated protein kinase phosphorylates and desensitizes smooth muscle myosin light chain kinase
    Sandrine Horman
    Hormone and Metabolic Research Unit, de Duve Institute, Universite Catholique de Louvain, B 1200 Brussels
    J Biol Chem 283:18505-12. 2008
    ..The findings suggest that AMPK attenuates contraction by phosphorylating and inactivating MLCK. This might contribute to reduced ATP turnover in the tonic phase of smooth muscle contraction...
  68. pmc Membrane depolarization-induced contraction of rat caudal arterial smooth muscle involves Rho-associated kinase
    Mitsuo Mita
    Department of Pharmacodynamics, Meiji Pharmaceutical University, 2 522 1 Noshio, Kiyose, Tokyo 204 8588, Japan
    Biochem J 364:431-40. 2002
    ..Furthermore, these results are consistent with inhibition of myosin light-chain phosphatase by ROK, which would account for the sustained elevation of myosin phosphorylation and tension in response to membrane depolarization...
  69. pmc Phosphorylation of the myosin phosphatase target subunit by integrin-linked kinase
    Andrea Murányi
    Muscle Biology Group, University of Arizona, Tucson, AZ 85721 0038, U S A
    Biochem J 366:211-6. 2002
    ..The findings that ILK phosphorylated both MYPT1 and myosin and the association of ILK with MP suggest that ILK may influence cytoskeletal structure or function...
  70. ncbi request reprint Phosphorylation of protein phosphatase type-1 inhibitory proteins by integrin-linked kinase and cyclic nucleotide-dependent protein kinases
    Ferenc Erdodi
    Department of Medical Chemistry, Medical and Health Science Center, University of Debrecen, H 4012 Debrecen, 18 B, Bem ter, Hungary
    Biochem Biophys Res Commun 306:382-7. 2003
    ..1 nM) and MPH (IC(50)=8 nM). These results suggest that the regulatory phosphorylation of CPI-17 and KEPI may involve distinct kinases and signaling pathways...