Sylvia van Drunen Littel-van den Hurk

Summary

Affiliation: University of Saskatchewan
Country: Canada

Publications

  1. ncbi request reprint Immunization with a dicistronic plasmid expressing a truncated form of bovine herpesvirus-1 glycoprotein D and the amino-terminal subunit of glycoprotein B results in reduced gB-specific immune responses
    Sharmila Manoj
    Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada
    Virology 313:296-307. 2003
  2. pmc Two doses of bovine viral diarrhea virus DNA vaccine delivered by electroporation induce long-term protective immune responses
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Clin Vaccine Immunol 20:166-73. 2013
  3. doi request reprint Pathogenesis of respiratory syncytial virus
    Sylvia van Drunen Littel-van den Hurk
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, SK, S7N 5E3, Canada
    Curr Opin Virol 2:300-5. 2012
  4. ncbi request reprint Cell-mediated immune responses induced by BHV-1: rational vaccine design
    Sylvia van Drunen Littel-van den Hurk
    University of Saskatchewan, Vaccine and Infectious Disease Organization, 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada
    Expert Rev Vaccines 6:369-80. 2007
  5. ncbi request reprint Immunopathology of RSV infection: prospects for developing vaccines without this complication
    S van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada
    Rev Med Virol 17:5-34. 2007
  6. doi request reprint Strategies for induction of protective immunity to bovine herpesvirus-1 in newborn calves with maternal antibodies
    S van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3
    Vaccine 26:3103-11. 2008
  7. ncbi request reprint Rationale and perspectives on the success of vaccination against bovine herpesvirus-1
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Sask, Canada S7N 5E3
    Vet Microbiol 113:275-82. 2006
  8. doi request reprint Electroporation-based DNA transfer enhances gene expression and immune responses to DNA vaccines in cattle
    S van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Vaccine 26:5503-9. 2008
  9. pmc Intranasal immunization of mice with a bovine respiratory syncytial virus vaccine induces superior immunity and protection compared to those by subcutaneous delivery or combinations of intranasal and subcutaneous prime-boost strategies
    John W Mapletoft
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada
    Clin Vaccine Immunol 17:23-35. 2010
  10. doi request reprint Electroporation for DNA immunization: clinical application
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, Microbiology and Immunology, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3, Canada
    Expert Rev Vaccines 9:503-17. 2010

Collaborators

Detail Information

Publications66

  1. ncbi request reprint Immunization with a dicistronic plasmid expressing a truncated form of bovine herpesvirus-1 glycoprotein D and the amino-terminal subunit of glycoprotein B results in reduced gB-specific immune responses
    Sharmila Manoj
    Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada
    Virology 313:296-307. 2003
    ..These results indicate that, although divalent plasmids may be used to express different antigens, the efficacy of vaccination with such plasmids may be influenced by the plasmid design and the characteristics of the expressed antigens...
  2. pmc Two doses of bovine viral diarrhea virus DNA vaccine delivered by electroporation induce long-term protective immune responses
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Clin Vaccine Immunol 20:166-73. 2013
    ..These results are promising and support this technology for use against infectious diseases in cattle and large species, including humans, in general...
  3. doi request reprint Pathogenesis of respiratory syncytial virus
    Sylvia van Drunen Littel-van den Hurk
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, SK, S7N 5E3, Canada
    Curr Opin Virol 2:300-5. 2012
    ..Ultimately, whether the response induced by RSV is protective or pathogenic depends on a combination of host factors, young age being one of the most important ones...
  4. ncbi request reprint Cell-mediated immune responses induced by BHV-1: rational vaccine design
    Sylvia van Drunen Littel-van den Hurk
    University of Saskatchewan, Vaccine and Infectious Disease Organization, 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada
    Expert Rev Vaccines 6:369-80. 2007
    ..This includes well-defined genetically engineered gene-deleted, subunit and vectored vaccines...
  5. ncbi request reprint Immunopathology of RSV infection: prospects for developing vaccines without this complication
    S van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada
    Rev Med Virol 17:5-34. 2007
    ..Maternal vaccination may be the optimal strategy to protect the very young infants...
  6. doi request reprint Strategies for induction of protective immunity to bovine herpesvirus-1 in newborn calves with maternal antibodies
    S van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3
    Vaccine 26:3103-11. 2008
    ..Close to complete protection against BHV-1 challenge was elicited in the calves immunized with the protein/CpG formulation, as these animals lost very little weight, had only slightly elevated temperatures and shed almost no virus...
  7. ncbi request reprint Rationale and perspectives on the success of vaccination against bovine herpesvirus-1
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Sask, Canada S7N 5E3
    Vet Microbiol 113:275-82. 2006
    ....
  8. doi request reprint Electroporation-based DNA transfer enhances gene expression and immune responses to DNA vaccines in cattle
    S van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Vaccine 26:5503-9. 2008
    ..Importantly, the TDS-based procedure was well tolerated by the calves, which did not need to be anesthetized or sedated. These results suggest that the TDS is a useful delivery method for DNA vaccines in cattle...
  9. pmc Intranasal immunization of mice with a bovine respiratory syncytial virus vaccine induces superior immunity and protection compared to those by subcutaneous delivery or combinations of intranasal and subcutaneous prime-boost strategies
    John W Mapletoft
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada
    Clin Vaccine Immunol 17:23-35. 2010
    ....
  10. doi request reprint Electroporation for DNA immunization: clinical application
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, Microbiology and Immunology, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, S7N 5E3, Canada
    Expert Rev Vaccines 9:503-17. 2010
    ..In support of the potential for electroporation in human disease situations, early clinical results suggest that the immunogenicity of DNA vaccines is greatly improved when delivered with electroporation...
  11. ncbi request reprint Immunization with plasmid DNA encoding a truncated, secreted form of the bovine viral diarrhea virus E2 protein elicits strong humoral and cellular immune responses
    Rong Liang
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Sask, Canada S7N 5E3
    Vaccine 23:5252-62. 2005
    ..Vaccination of cattle confirmed that pMASIA-tPAsDeltaE2 elicited both strong humoral and cellular immune responses and thus could be a candidate DNA vaccine against BVDV...
  12. ncbi request reprint Immunity and protection by adoptive transfer of dendritic cells transfected with hepatitis C NS3/4A mRNA
    Hong Yu
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Sask S7N 5E3, Canada
    Vaccine 25:1701-11. 2007
    ..Since no study has explored the in vivo efficacy of mRNA-transfected DC-mediated vaccination against viral diseases, including hepatitis C, our study provided a novel vaccination strategy against hepatitis C as well as other pathogens...
  13. pmc Modulation of immune responses to bovine herpesvirus-1 in cattle by immunization with a DNA vaccine encoding glycoprotein D as a fusion protein with bovine CD154
    Sharmila Manoj
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Immunology 112:328-38. 2004
    ....
  14. doi request reprint Major tegument protein VP8 of bovine herpesvirus 1 is phosphorylated by viral US3 and cellular CK2 protein kinases
    Shaunivan L Labiuk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada
    J Gen Virol 90:2829-39. 2009
    ..Based on these results, it is proposed that VP8 interacts with cellular CK2 and viral US3 in BoHV-1-infected cells, and is in turn subject to kinase activities associated with both of these proteins...
  15. doi request reprint Intracellular trafficking of VP22 in bovine herpesvirus-1 infected cells
    Vladislav A Lobanov
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada
    Virology 396:189-202. 2010
    ....
  16. ncbi request reprint Intranasal immunization of mice with a formalin-inactivated bovine respiratory syncytial virus vaccine co-formulated with CpG oligodeoxynucleotides and polyphosphazenes results in enhanced protection
    John W Mapletoft
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    J Gen Virol 89:250-60. 2008
    ..Co-formulation of CpG ODN and PP is a promising new vaccine platform technology that may have applications in mucosal immunization in humans...
  17. doi request reprint Electroporation enhances immune responses and protection induced by a bovine viral diarrhea virus DNA vaccine in newborn calves with maternal antibodies
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada
    Vaccine 28:6445-54. 2010
    ....
  18. pmc Bovine herpesvirus 1 VP22 enhances the efficacy of a DNA vaccine in cattle
    Chunfu Zheng
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, SK S7N 5E3, Canada
    J Virol 79:1948-53. 2005
    ..This is the first report of the use of VP22 as a transport molecule in the context of a DNA vaccine for a large animal species...
  19. doi request reprint Bovine herpesvirus-1 VP8 interacts with DNA damage binding protein-1 (DDB1) and is monoubiquitinated during infection
    Natalya L Vasilenko
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Virus Res 167:56-66. 2012
    ..These data suggest that BHV-1 VP8 interacts with DDB1-CUL4 E3 ubiquitin ligase, which correlates to monoubiquitination of this viral protein...
  20. ncbi request reprint A single DNA immunization in combination with electroporation prolongs the primary immune response and maintains immune memory for six months
    Cemaine Tsang
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada
    Vaccine 25:5485-94. 2007
    ..Therefore, an effective primary DNA vaccination has the potential to significantly prolong the duration of an antibody response and possibly reduce the frequency of revaccination...
  21. ncbi request reprint Formulation with CpG oligodeoxynucleotides increases cellular immunity and protection induced by vaccination of calves with formalin-inactivated bovine respiratory syncytial virus
    John W Mapletoft
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, 120 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 5E3
    Virology 353:316-23. 2006
    ..Based on these observations, CpG ODN appears to be a suitable candidate adjuvant for inactivated BRSV vaccines...
  22. ncbi request reprint Compatibility of plasmids encoding bovine viral diarrhea virus type 1 and type 2 E2 in a single DNA vaccine formulation
    Rong Liang
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, 120 Veterinary Road, Saskatoon, Sask S7N 5E3, Canada
    Vaccine 25:5994-6006. 2007
    ..Thus, a mixture of plasmids encoding type 1 and type 2 E2 could be a potential DNA vaccine candidate against both BVDV1 and BVDV2...
  23. doi request reprint Bovine herpesvirus-1 US3 protein kinase: critical residues and involvement in the phosphorylation of VP22
    Shaunivan L Labiuk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada
    J Gen Virol 91:1117-26. 2010
    ..These data demonstrate that BoHV-1 VP22 interacts with both CK2 and US3, and that CK2 is the major kinase phosphorylating VP22, with US3 playing a minor role...
  24. ncbi request reprint Priming with DNA encoding E2 and boosting with E2 protein formulated with CpG oligodeoxynucleotides induces strong immune responses and protection from Bovine viral diarrhea virus in cattle
    Rong Liang
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, SK S7N 5E3, Canada
    J Gen Virol 87:2971-82. 2006
    ..These results demonstrate the advantage of a DNA prime-protein boost vaccination approach in an outbred species...
  25. ncbi request reprint A single HBsAg DNA vaccination in combination with electroporation elicits long-term antibody responses in sheep
    Shawn Babiuk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    Bioelectrochemistry 70:269-74. 2007
    ..Thus, a single DNA vaccination, in combination with electroporation, approached the efficacy of the commercial subunit vaccine in the maintenance of long-term protective serum antibody titres and immune memory...
  26. pmc Characterization of the nuclear localization and nuclear export signals of bovine herpesvirus 1 VP22
    Chunfu Zheng
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, Saskatchewan S7N 5E3, Canada
    J Virol 79:11864-72. 2005
    ..This demonstrates that this leucine-rich motif is a functional NES. These data represent the first identification of a functional NLS and NES in a herpesvirus VP22 homologue...
  27. pmc TLR9-/- and TLR9+/+ mice display similar immune responses to a DNA vaccine
    Shawn Babiuk
    Pyxis Genomics Canada, Saskatoon, Saskatchewan, Canada
    Immunology 113:114-20. 2004
    ..This study illustrates that DNA vaccines elicit immune responses by multiple mechanisms and demonstrates that TLR9 is not essential for the induction of immune responses following DNA immunization...
  28. doi request reprint A novel triple adjuvant formulation promotes strong, Th1-biased immune responses and significant antigen retention at the site of injection
    Heather L Wilson
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada
    Vaccine 28:8288-99. 2010
    ..These results indicate that the CpG/Indol/PP4 combination promotes prolonged antigen retention and strong, antigen-specific Th1-biased immune responses...
  29. pmc A UL47 gene deletion mutant of bovine herpesvirus type 1 exhibits impaired growth in cell culture and lack of virulence in cattle
    Vladislav A Lobanov
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5E3, Canada
    J Virol 84:445-58. 2010
    ....
  30. ncbi request reprint Needle-free delivery of veterinary DNA vaccines
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Sakatoon, Saskatchewan, Canada
    Methods Mol Med 127:91-105. 2006
    ..Furthermore, we will describe the delivery of vaccines to mucosal surfaces. The procedures described will use sheep and cattle as model veterinary species...
  31. ncbi request reprint Effect of different nuclear localization sequences on the immune responses induced by a MIDGE vector encoding bovine herpesvirus-1 glycoprotein D
    Chunfu Zheng
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, SK, Canada S7N 5E3
    Vaccine 24:4625-9. 2006
    ..The NLS from BHV-1 VP8 was most efficient at enhancing the tgD production and tgD-specific immune responses when conjugated to the MIDGE-tgD vector...
  32. doi request reprint Approaches to enhancing immune responses stimulated by CpG oligodeoxynucleotides
    George Mutwiri
    Vaccine and Infectious Disease Organization International Vaccine Center, University of Saskatchewan, SK, Canada
    Adv Drug Deliv Rev 61:226-32. 2009
    ..We will discuss these approaches and the mechanisms involved, with emphasis on what we have learned from large animal models...
  33. doi request reprint The response of aged mice to primary infection and re-infection with pneumonia virus of mice depends on their genetic background
    Pratima Shrivastava
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan S7N 5E3, Canada
    Immunobiology 221:494-502. 2016
    ..However, the central memory CD4(+) and CD8(+) T cells were significantly enhanced in numbers in the lungs and draining lymph nodes of both mouse strains after re-infection, and these numbers were higher for C57BL/6 mice. ..
  34. ncbi request reprint Priming with CpG-enriched plasmid and boosting with protein formulated with CpG oligodeoxynucleotides and Quil A induces strong cellular and humoral immune responses to hepatitis C virus NS3
    Hong Yu
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK, Canada S7N 5E3
    J Gen Virol 85:1533-43. 2004
    ..These results demonstrate the advantage of a DNA prime-protein boost approach in inducing a strong NS3-specific cell-mediated, as well as humoral, immune response, in both inbred laboratory and outbred large animal species...
  35. ncbi request reprint Dendritic cells pulsed with hepatitis C virus NS3 protein induce immune responses and protection from infection with recombinant vaccinia virus expressing NS3
    Hong Yu
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada
    J Gen Virol 87:1-10. 2006
    ....
  36. doi request reprint Multiple molecular regions confer intracellular localization of bovine Toll-like receptor 8
    Jianzhong Zhu
    Vaccine and Infectious Disease Organization VIDO, University of Saskatchewan, Saskatoon, Saskatchewan, Canada S7N 5E3
    Mol Immunol 46:884-92. 2009
    ..Our results suggest that multiple regions, including ECD, TM, linker and TIR-tail regions of bTLR8, are involved in determining the localization of cellular ER compartment...
  37. ncbi request reprint Targeting with bovine CD154 enhances humoral immune responses induced by a DNA vaccine in sheep
    Sharmila Manoj
    Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada
    J Immunol 170:989-96. 2003
    ..These results demonstrate that using CD154 to target plasmid-expressed Ag can significantly enhance immune responses induced by a DNA vaccine...
  38. doi request reprint The role of dendritic cells in innate and adaptive immunity to respiratory syncytial virus, and implications for vaccine development
    Ravendra Garg
    VIDO Intervac, University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada
    Expert Rev Vaccines 11:1441-57. 2012
    ..Furthermore, stimulation of DCs through Toll-like receptors is essential for induction of protective immunity to RSV. These characteristics have implications for the rational design of a RSV vaccine...
  39. doi request reprint The synthetic peptides bovine enteric β-defensin (EBD), bovine neutrophil β-defensin (BNBD) 9 and BNBD 3 are chemotactic for immature bovine dendritic cells
    Sarah Mackenzie-Dyck
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada
    Vet Immunol Immunopathol 143:87-107. 2011
    ..These results are the first to describe chemotactic ability of synthetic bovine β-defensins for immature bovine monocyte-derived DCs...
  40. pmc Immunogenicity of a bovine herpesvirus 1 glycoprotein D DNA vaccine complexed with bovine neutrophil beta-defensin 3
    Sarah Mackenzie-Dyck
    VIDO Intervac, University of Saskatchewan, Saskatoon, Canada Veterinary Microbiology, University of Saskatchewan, Saskatoon, Canada
    Clin Vaccine Immunol 22:79-90. 2015
    ..Native, synthetic, and analog forms of BNBD3 were equally capable of inducing functional maturation of BMDCs. ..
  41. ncbi request reprint High transfection efficiency, gene expression, and viability of monocyte-derived human dendritic cells after nonviral gene transfer
    Abdolamir Landi
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, SK, S7N 5E3, Canada
    J Leukoc Biol 82:849-60. 2007
    ..Furthermore, the IVT RNA-transfected iDCs retained all phenotypic and functional characteristics of iDCs. This method is applicable to most purposes, including in vitro functional assays, in vivo DC immunotherapy, and DC-based vaccines...
  42. doi request reprint Dendritic cells matured by a prostaglandin E2-containing cocktail can produce high levels of IL-12p70 and are more mature and Th1-biased than dendritic cells treated with TNF-α or LPS
    Abdolamir Landi
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Rd, Saskatoon, SK S7N5E3, Canada
    Immunobiology 216:649-62. 2011
    ..More interestingly, the Th1-promoting characteristics of MC-mDCs were confirmed by their potential to produce large amounts of IL-12p70 after effective stimulation simulating in vivo events...
  43. ncbi request reprint Strategies for improved formulation and delivery of DNA vaccines to veterinary target species
    Sylvia van Drunen Littel-van den Hurk
    Vaccine and Infectious Disease Organisation, University of Saskatchewan, Saskatoon, SK, Canada
    Immunol Rev 199:113-25. 2004
    ..Hopefully, transfection efficiency can be increased further in a user-friendly manner to ensure that the benefits of using DNA vaccines become a reality...
  44. ncbi request reprint Approaches to enhance the efficacy of DNA vaccines
    Sharmila Manoj
    Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada
    Crit Rev Clin Lab Sci 41:1-39. 2004
    ..The overall conclusions from these studies suggest that, provided these critical improvements are made, DNA vaccines may find important clinical and practical applications in the field of vaccination...
  45. doi request reprint IL-12p40 gene-deficient BALB/c mice exhibit lower weight loss, reduced lung pathology and decreased sensitization to allergen in response to infection with pneumonia virus of mice
    Pratima Shrivastava
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 5E3
    Virology 497:1-10. 2016
    ..In conclusion, these data suggest that a depressed regulatory capacity in BALB/c mice to PVM infection results in enhanced immunopathology and sensitization to allergen. ..
  46. doi request reprint The bovine viral diarrhea virus E2 protein formulated with a novel adjuvant induces strong, balanced immune responses and provides protection from viral challenge in cattle
    Marlene Snider
    VIDO Intervac, University of Saskatchewan, Saskatoon, SK, Canada S7N 5E3
    Vaccine 32:6758-64. 2014
    ..These data suggest that this E2 subunit formulation induces significant protection from BVDV-2 challenge, and thus is a promising BVDV vaccine candidate; in addition, the adjuvant platform has applications in bovine vaccines in general. ..
  47. pmc Inclusion of the bovine neutrophil beta-defensin 3 with glycoprotein D of bovine herpesvirus 1 in a DNA vaccine modulates immune responses of mice and cattle
    Sarah Mackenzie-Dyck
    VIDO Intervac, University of Saskatchewan, Saskatoon, Canada
    Clin Vaccine Immunol 21:463-77. 2014
    ..These data show that coadministration of BNBD3 with a protective antigen as a fusion in a DNA vaccine strengthened the Th1 bias and increased cell-mediated immune responses but did not enhance protection from BoHV-1 infection. ..
  48. doi request reprint Carrier molecules for use in veterinary vaccines
    Volker Gerdts
    Vaccine and Infectious Disease Organization International Vaccine Centre, University of Saskatchewan, Saskatoon, SK, Canada
    Vaccine 31:596-602. 2013
    ....
  49. pmc Innate and adaptive immune response to pneumonia virus of mice in a resistant and a susceptible mouse strain
    Ellen R T Watkiss
    VIDO Intervac, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E3, Canada
    Viruses 5:295-320. 2013
    ..Overall, the difference in susceptibility of these two strains appeared to be related not to an inherent T helper bias, but to the capacity of the C57Bl/6 mice to control both viral replication and the immune response elicited by PVM...
  50. pmc A distinct role of CD4+ Th17- and Th17-stimulated CD8+ CTL in the pathogenesis of type 1 diabetes and experimental autoimmune encephalomyelitis
    Manjunatha Ankathatti Munegowda
    Research Unit, Saskatchewan Cancer Agency, Department of Oncology, Saskatoon Cancer Center, 20 Campus Drive, Saskatoon, Saskatchewan, S7N 4H4, Canada
    J Clin Immunol 31:811-26. 2011
    ....
  51. doi request reprint Combination adjuvants: the next generation of adjuvants?
    George Mutwiri
    Vaccines and Infectious Disease Organization International Vaccine Center, University of Saskatchewan, Saskatoon, Saskatchewan, S7N 5E3, Canada
    Expert Rev Vaccines 10:95-107. 2011
    ..This approach is promising and presents tremendous opportunities for vaccinologists to tailor immune responses to specific vaccines. In this article, adjuvant combinations at different stages of development will be reviewed...
  52. pmc Vaccine Potentiation by Combination Adjuvants
    Benoit Levast
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Vaccines (Basel) 2:297-322. 2014
    ..Several of these combination adjuvants are promising having promoted improved and balanced immune responses. ..
  53. pmc Phosphorylation of Bovine Herpesvirus 1 VP8 Plays a Role in Viral DNA Encapsidation and Is Essential for Its Cytoplasmic Localization and Optimal Virion Incorporation
    Kuan Zhang
    VIDO Intervac, University of Saskatchewan, Saskatoon, SK, Canada Vaccinology and Immunotherapeutics, University of Saskatchewan, Saskatoon, SK, Canada
    J Virol 90:4427-40. 2016
    ..To perform these functions, the cellular localization of VP8 is adjusted based on the phosphorylation status...
  54. doi request reprint Intranasal treatment with a novel immunomodulator mediates innate immune protection against lethal pneumonia virus of mice
    Elisa C Martinez
    Department of Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, 107 Wiggins Road, S7N 5E5, Canada Vaccine and Infectious Disease Organization International Vaccine Centre VIDO InterVac, University of Saskatchewan, Saskatoon, Saskatchewan, 120 Veterinary Road, S7N 5E3, Canada
    Antiviral Res 135:108-119. 2016
    ..On day 14 post-infection, P-I-P-treated mice were confirmed to be PVM-free. These results demonstrate the capacity of this formulation to prevent PVM and possibly other viral respiratory infections...
  55. doi request reprint Blunted inflammatory and mucosal IgA responses to pneumonia virus of mice in C57BL/6 neonates are correlated to reduced protective immunity upon re-infection as elderly mice
    Pratima Shrivastava
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 5E3
    Virology 485:233-43. 2015
    ..These results suggest that an effective local innate response, as well as priming of mucosal adaptive responses in neonates after PVM-15 infection is correlated to decreased susceptibility and protection upon re-infection. ..
  56. pmc Regulation and function of phosphorylation on VP8, the major tegument protein of bovine herpesvirus 1
    Kuan Zhang
    VIDO Intervac, University of Saskatchewan, Saskatoon, Saskatchewan, Canada Vaccinology and Immunotherapeutics, University of Saskatchewan, Saskatoon, Saskatchewan, Canada
    J Virol 89:4598-611. 2015
    ..This suggests that VP8 plays a role in resisting PML-related host antiviral defenses by redistributing PML protein and that this function depends on the phosphorylation of VP8...
  57. pmc Respiratory disease caused by Mycoplasma bovis is enhanced by exposure to bovine herpes virus 1 (BHV-1) but not to bovine viral diarrhea virus (BVDV) type 2
    Tracy Prysliak
    Vaccine and Infectious Disease Organization, Saskatoon, Saskatchewan, Canada
    Can Vet J 52:1195-202. 2011
    ..We conclude that previous exposure to BHV-1 but not BVDV type 2 was a necessary cause of M. bovis-related respiratory diseases in our disease model...
  58. ncbi request reprint Characterization of nuclear localization and export signals of the major tegument protein VP8 of bovine herpesvirus-1
    Chunfu Zheng
    Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, Saskatchewan, Canada S7N 5E3
    Virology 324:327-39. 2004
    ..This leucine-rich NES caused transport of YFP to the cytoplasm. These results demonstrate that VP8 shuttles between the nucleus and cytoplasm...
  59. pmc VP8, the Major Tegument Protein of Bovine Herpesvirus 1, Interacts with Cellular STAT1 and Inhibits Interferon Beta Signaling
    Sharmin Afroz
    VIDO Intervac, University of Saskatchewan, Saskatoon, SK, Canada Vaccinology and Immunotherapeutics, University of Saskatchewan, Saskatoon, SK, Canada
    J Virol 90:4889-904. 2016
    ..These results suggest that viral VP8 downregulates IFN-β signaling early during infection, thus playing a role in overcoming the antiviral response of BoHV-1-infected cells...
  60. doi request reprint Formulation of the respiratory syncytial virus fusion protein with a polymer-based combination adjuvant promotes transient and local innate immune responses and leads to improved adaptive immunity
    Indranil Sarkar
    VIDO Intervac, University of Saskatchewan, Saskatoon S7N 5E3, Canada Microbiology and Immunology, University of Saskatchewan, Saskatoon S7N 5E5, Canada
    Vaccine 34:5114-24. 2016
    ....
  61. doi request reprint A single intranasal immunization with a subunit vaccine formulation induces higher mucosal IgA production than live respiratory syncytial virus
    Ravendra Garg
    VIDO Intervac, University of Saskatchewan, Saskatoon, Canada SK S7N 5E3
    Virology 499:288-297. 2016
    ....
  62. doi request reprint Interaction of VP8 with mRNAs of bovine herpesvirus-1
    Azharul Islam
    VIDO Intervac, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada
    Virus Res 197:116-26. 2015
    ..Based on these results, we concluded that VP8 binds to intron-less mRNAs of bICP0, gB, gC and gD...
  63. ncbi request reprint Synthetic peptide vaccination in cattle: induction of strong cellular immune responses against peptides derived from the Mycobacterium bovis antigen Rv3019c
    H Martin Vordermeier
    Veterinary Labortories Agency Weybridge, TB Research Group, New Haw, Addlestone, Surrey KT15 3NB, UK
    Vaccine 23:4375-84. 2005
    ..Peptide vaccination boosted responses primed by DNA vaccination. In conclusion, emulsigen and CpG motif containing ODN constitute a promising adjuvant formulation to deliver peptides to veterinary species...
  64. pmc Vaccination of cattle with a CpG oligodeoxynucleotide-formulated mycobacterial protein vaccine and Mycobacterium bovis BCG induces levels of protection against bovine tuberculosis superior to those induced by vaccination with BCG alone
    D Neil Wedlock
    AgResearch, Wallaceville Animal Research Centre, P O Box 40063, Upper Hutt, New Zealand
    Infect Immun 73:3540-6. 2005
    ..These results suggest that a combination of a CpG ODN-formulated protein vaccine and BCG offers better protection against bovine tuberculosis than does BCG alone...
  65. ncbi request reprint Phylogeny and antigenic relationships of three cervid herpesviruses
    Dirk Deregt
    Virology Section, Lethbridge Laboratory Animal Diseases Research Institute, Canadian Food Inspection Agency, P O Box 640, Lethbridge, Alta, Canada T1J 3Z4
    Virus Res 114:140-8. 2005
    ..The results indicate that ElkHV and CerHV-1 have diverged from a common ancestor virus. Cervid herpesviruses may be useful in determination of evolutionary rates of change for alphaherpesvirus genes...
  66. ncbi request reprint Delivery of DNA vaccines using electroporation
    Shawn Babiuk
    Canadian Food Inspection Agency, Government of Canada, Winnipeg, Manitoba, Canada
    Methods Mol Med 127:73-82. 2006
    ..A clear concern is balancing the plasmid uptake with cellular or tissue damage. Techniques are described to achieve this goal...