A M James Shapiro

Summary

Affiliation: University of Alberta
Country: Canada

Publications

  1. ncbi Clinical islet transplant: current and future directions towards tolerance
    A M James Shapiro
    Clinical Islet Transplant Program and Department of Surgery, University of Alberta, 200 College Plaza, 8215 112 Street, Edmonton, Alberta, Canada T6G 2C8
    Immunol Rev 196:219-36. 2003
  2. pmc Glutathione ethyl ester supplementation during pancreatic islet isolation improves viability and transplant outcomes in a murine marginal islet mass model
    Alexandre S Raposo do Amaral
    Alberta Diabetes Institute, University of Alberta, Edmonton AB, Canada
    PLoS ONE 8:e55288. 2013
  3. pmc The use of bovine pericardial buttress on linear stapler fails to reduce pancreatic fistula incidence in a porcine pancreatic transection model
    A MacIver
    Department of Surgery, University of Alberta Hospitals, 2000 College Plaza, 8215 112 Street, Edmonton, Alberta, Canada T6G 2C8
    HPB Surg 2011:624060. 2011
  4. pmc Spontaneous Hepatic Rupture with Intraperitoneal Hemorrhage without Underlying Etiology: A Report of Two Cases
    Kristin Klein
    Department of Surgery, University of Alberta, 2000 College Plaza, 8215 112th Street, Edmonton, AB, Canada T6G 2C8
    ISRN Surg 2011:610747. 2011
  5. pmc Islet transplantation in type 1 diabetes: ongoing challenges, refined procedures, and long-term outcome
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, 2000 College Plaza, 8215 112th Street, Edmonton AB Canada T6G 2C8
    Rev Diabet Stud 9:385-406. 2012
  6. pmc Strategies toward single-donor islets of Langerhans transplantation
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Curr Opin Organ Transplant 16:627-31. 2011
  7. ncbi Defining optimal immunosuppression for islet transplantation based on reduced diabetogenicity in canine islet autografts
    A M James Shapiro
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1522-8. 2002
  8. doi State of the art of clinical islet transplantation and novel protocols of immunosuppression
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB T6G 2C8, Canada
    Curr Diab Rep 11:345-54. 2011
  9. ncbi Islet isolation and transplantation outcomes of pancreas preserved with University of Wisconsin solution versus two-layer method using preoxygenated perfluorocarbon
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health, Edmonton, Alberta, Canada
    Transplantation 82:1286-90. 2006
  10. doi Supplemental islet infusions restore insulin independence after graft dysfunction in islet transplant recipients
    Angela Koh
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada
    Transplantation 89:361-5. 2010

Detail Information

Publications101 found, 100 shown here

  1. ncbi Clinical islet transplant: current and future directions towards tolerance
    A M James Shapiro
    Clinical Islet Transplant Program and Department of Surgery, University of Alberta, 200 College Plaza, 8215 112 Street, Edmonton, Alberta, Canada T6G 2C8
    Immunol Rev 196:219-36. 2003
    ....
  2. pmc Glutathione ethyl ester supplementation during pancreatic islet isolation improves viability and transplant outcomes in a murine marginal islet mass model
    Alexandre S Raposo do Amaral
    Alberta Diabetes Institute, University of Alberta, Edmonton AB, Canada
    PLoS ONE 8:e55288. 2013
    ..The increased generation of reactive oxygen species (ROS) during islet isolation and the consumption of antioxidant defenses appear to be an important pathway related to islet damage...
  3. pmc The use of bovine pericardial buttress on linear stapler fails to reduce pancreatic fistula incidence in a porcine pancreatic transection model
    A MacIver
    Department of Surgery, University of Alberta Hospitals, 2000 College Plaza, 8215 112 Street, Edmonton, Alberta, Canada T6G 2C8
    HPB Surg 2011:624060. 2011
    ..Conclusion. Buttressed stapler failed to protect against pancreatic fistula in this rigorous surgical model...
  4. pmc Spontaneous Hepatic Rupture with Intraperitoneal Hemorrhage without Underlying Etiology: A Report of Two Cases
    Kristin Klein
    Department of Surgery, University of Alberta, 2000 College Plaza, 8215 112th Street, Edmonton, AB, Canada T6G 2C8
    ISRN Surg 2011:610747. 2011
    ..Spontaneous hepatic rupture is a rare event most often occurring in association with underlying liver disease or pregnancy. We report two unusual cases of hepatic rupture without any identifiable pathology, trauma, or comorbid conditions...
  5. pmc Islet transplantation in type 1 diabetes: ongoing challenges, refined procedures, and long-term outcome
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, 2000 College Plaza, 8215 112th Street, Edmonton AB Canada T6G 2C8
    Rev Diabet Stud 9:385-406. 2012
    ..Induction of immunological tolerance to self-islet antigens and to allografts is a difficult challenge, but potentially within our grasp. ..
  6. pmc Strategies toward single-donor islets of Langerhans transplantation
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Curr Opin Organ Transplant 16:627-31. 2011
    ..This point is important as further islet or pancreas transplants in the remote future or the potential future need for a solid organ kidney transplant may become prohibitive if the recipient is sensitized...
  7. ncbi Defining optimal immunosuppression for islet transplantation based on reduced diabetogenicity in canine islet autografts
    A M James Shapiro
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1522-8. 2002
    ..We here explore the effects of clinically relevant doses of cyclosporine or tacrolimus when given alone or in combination with glucocorticoids on long-term canine islet autograft function...
  8. doi State of the art of clinical islet transplantation and novel protocols of immunosuppression
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB T6G 2C8, Canada
    Curr Diab Rep 11:345-54. 2011
    ..We review the current status of islet transplantation, and discuss current and future immunosuppressive protocols that will pave the way to more broad application of cellular replacement in diabetes...
  9. ncbi Islet isolation and transplantation outcomes of pancreas preserved with University of Wisconsin solution versus two-layer method using preoxygenated perfluorocarbon
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health, Edmonton, Alberta, Canada
    Transplantation 82:1286-90. 2006
    ..However, the effect of TLM has not been evaluated in large-scale study. In addition, a direct benefit of TLM on islet transplantation outcome has not been addressed in the setting of any randomized controlled trials...
  10. doi Supplemental islet infusions restore insulin independence after graft dysfunction in islet transplant recipients
    Angela Koh
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada
    Transplantation 89:361-5. 2010
    ..However, improving islet transplant outcomes could explain the success of SII. We aimed to determine the effect on islet graft function and insulin independence of SII using these new protocols, without the use of exenatide...
  11. doi Nonsimultaneous administration of pancreas dissociation enzymes during islet isolation
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Alberta Health Services, Edmonton, Alberta, Canada
    Transplantation 87:1700-5. 2009
    ..However, this conversion significantly affected the field in transplant activity. Here, we report our successful conversion from Liberase to Serva collagenase with the use of a modified digestion protocol...
  12. pmc Caspase inhibitor therapy synergizes with costimulation blockade to promote indefinite islet allograft survival
    Juliet A Emamaullee
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Diabetes 59:1469-77. 2010
    ..Costimulation blockade has emerged as a selective nontoxic maintenance therapy in transplantation. However, these drugs must be combined with other immunomodulatory agents to ensure long-term graft survival...
  13. ncbi Costimulation blockade of both inducible costimulator and CD40 ligand induces dominant tolerance to islet allografts and prevents spontaneous autoimmune diabetes in the NOD mouse
    Sulaiman A Nanji
    Roberts Centre, 2000 College Plaza, University of Alberta, 2000 College Plaza, 8215 112 St, Edmonton, Alberta, Canada T6G 2C8
    Diabetes 55:27-33. 2006
    ..These data demonstrate that the blockade of CD40 ligand and ICOS signaling induces islet allograft tolerance involving a dominant mechanism associated with intragraft regulatory cells and prevents autoimmune diabetes in NOD mice...
  14. ncbi Ameliorating injury during preservation and isolation of human islets using the two-layer method with perfluorocarbon and UW solution
    Payam Salehi
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Cell Transplant 15:187-94. 2006
    ....
  15. ncbi Influence of pancreas preservation on human islet isolation outcomes: impact of the two-layer method
    Toshiaki Tsujimura
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Transplantation 78:96-100. 2004
    ..The purpose of this study was to clarify the limitations of the University of Wisconsin (UW) solution and the potentials of the two-layer method (TLM) for pancreas preservation before human islet isolation...
  16. ncbi Robust tolerance to fully allogeneic islet transplants achieved by chimerism with minimal conditioning
    Bin Luo
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Canada
    Transplantation 80:370-7. 2005
    ..Here we investigate whether our nonmyeloablative, costimulation blockade-free and sirolimus (SRL)-based protocol could facilitate mixed chimerism via bone marrow transplantation (BMT) and induce islet allograft tolerance...
  17. ncbi De novo sirolimus-based immunosuppression after liver transplantation for hepatocellular carcinoma: long-term outcomes and side effects
    Christian Toso
    Department of Surgery, Section of Hepatobiliary, Pancreatic and Transplant Surgery, University of Alberta, Edmonton, Canada
    Transplantation 83:1162-8. 2007
    ..We report long-term outcomes and side effects after transplantation for hepatocellular carcinoma (HCC) using de novo, sirolimus-based immunosuppression (IS)...
  18. ncbi Long-term graft function after allogeneic islet transplantation
    Jonathan R T Lakey
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 16:441-6. 2007
    ..In contrast, sustained insulin secretion with nearly normal HbA1c at 13 years follow-up was observed in another patient, providing hope for improving long-term graft outcomes for islet transplant recipient...
  19. doi The caspase selective inhibitor EP1013 augments human islet graft function and longevity in marginal mass islet transplantation in mice
    Juliet A Emamaullee
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Diabetes 57:1556-66. 2008
    ..EP1013 (N-benzyloxycabonyl-Val Asp-fluoromethyl ketone [zVD-FMK]), is a broad-spectrum caspase selective inhibitor with no observed toxicity in rodents...
  20. doi Protein kinase C inhibitor, AEB-071, acts complementarily with cyclosporine to prevent islet rejection in rats
    Shaheed Merani
    Department of Surgery, Surgical Medical Research Institute, University of Alberta, Edmonton, AB, Canada
    Transplantation 87:59-65. 2009
    ..The present study investigated the effect of AEB on rat islet allotransplantation alone or in combination with CTLA4-Ig, mycophenolate mofetil, or cyclosporine A (CsA)...
  21. doi Histologic graft assessment after clinical islet transplantation
    Christian Toso
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada
    Transplantation 88:1286-93. 2009
    ..An accurate monitoring would help understanding the fate of islet grafts after transplantation...
  22. doi Comparison of human islet isolation outcomes using a new mammalian tissue-free enzyme versus collagenase NB-1
    Doug O'Gorman
    Department of Transplant Services, Alberta Health Services, Edmonton, AB, Canada
    Transplantation 90:255-9. 2010
    ..We report our experience using the MTF enzyme in clinical islet isolations compared with Serva NB-1 with modified enzyme delivery method...
  23. doi AEB071 (sotrastaurin) does not exhibit toxic effects on human islets in vitro, nor after transplantation into immunodeficient mice
    Shaheed Merani
    Alberta Diabetes Institute, University of Alberta, Edmonton, Alberta, Canada
    Islets 3:338-43. 2011
    ..These results suggest that AEB is not associated with toxic effects on islet engraftment or function. AEB appears to be an appropriate immunosuppressive candidate for clinical trials in islet transplantation...
  24. doi Insulin-heparin infusions peritransplant substantially improve single-donor clinical islet transplant success
    Angela Koh
    1 Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada 2 Division of Clinical Immunology, Department of Oncology, Radiology and Clinical Immunology, The Rudbeck Laboratory, Uppsala University, Uppsala, Sweden
    Transplantation 89:465-71. 2010
    ..The ability to achieve insulin independence with a single donor is an important goal in clinical islet transplantation due to the limited organ supply...
  25. doi Liraglutide, a long-acting human glucagon-like peptide 1 analogue, improves human islet survival in culture
    Christian Toso
    Clinical Islet Transplant Program, Department of Surgery, University of Alberta, Edmonton, AB, Canada
    Transpl Int 23:259-65. 2010
    ..Overall, these data demonstrate the beneficial effect of liraglutide on cultured human islets, preserving islet mass. They support the design of clinical studies looking at the effect of liraglutide in clinical islet transplantation...
  26. ncbi Short-term storage of the ischemically damaged human pancreas by the two-layer method prior to islet isolation
    Toshiaki Tsujimura
    Surgical Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Alberta T6G2N8, Canada
    Cell Transplant 13:67-73. 2004
    ..Use of the TLM following prolonged storage in UW provides an excellent adjunctive protocol for treating human pancreata for the rigors of the islet isolation process...
  27. doi BTLA targeting modulates lymphocyte phenotype, function, and numbers and attenuates disease in nonobese diabetic mice
    Wayne Truong
    Surgical Medical Research Institute, Department of Surgery, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada
    J Leukoc Biol 86:41-51. 2009
    ..Hence, strategies targeting BTLA+ lymphocytes or therapies enhancing the BTLA-negative cosignal may prove valuable in treating autoimmune diabetes...
  28. ncbi Development of either split tolerance or robust tolerance along with humoral tolerance to donor and third-party alloantigens in nonmyeloablative mixed chimeras
    William F N Chan
    Department of Medical Microbiology and Immunology, Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    J Immunol 180:5177-86. 2008
    ..In contrast, the possibility of humoral tolerance to multiple donors is potentially beneficial...
  29. ncbi Five-year follow-up after clinical islet transplantation
    Edmond A Ryan
    Department of Medicine, Clinical Islet Transplant Program, 2000 College Plaza, 8215 112th St, Edmonton, Alberta, Canada T6G 2C8
    Diabetes 54:2060-9. 2005
    ..The results, though promising, still point to the need for further progress in the availability of transplantable islets, improving islet engraftment, preserving islet function, and reducing toxic immunosuppression...
  30. pmc Long-term follow-up of hepatic ultrasound findings in subjects with magnetic resonance imaging defined hepatic steatosis following clinical islet transplantation: a case-control study
    Stephanie Jackson
    Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB Canada
    Islets 5:16-21. 2013
    ..6 ± 2.0 years. In five cases steatosis resolved, with recurrence in two cases during the follow-up period (8.5 ± 3.2 years). No evidence of CIT causing deleterious effects on long-term liver function or graft outcome was observed...
  31. ncbi Estimation of pancreas weight from donor variables
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 15:181-5. 2006
    ..These formulae should contribute to the estimation of pancreas weight, and thus improve donor selection for islet isolation and transplantation...
  32. doi Belatacept and basiliximab diminish human antiporcine xenoreactivity and synergize to inhibit alloimmunity
    Juliet A Emamaullee
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 85:118-24. 2008
    ..In the present study, in vitro experiments were conducted to determine if these agents could synergize to inhibit immune responses...
  33. ncbi Coinhibitory T-cell signaling in islet allograft rejection and tolerance
    Wayne Truong
    Department of Surgery, Faculty of Medicine, University of Alberta, Edmonton, AB, Canada
    Cell Transplant 15:105-19. 2006
    ..Tolerance induction will likely rely on understanding the balance of positive and negative signals affecting the state of T-cell activation...
  34. doi The caspase inhibitor IDN-6556 (PF3491390) improves marginal mass engraftment after islet transplantation in mice
    Michael McCall
    Department of Surgery, University of Alberta, Edmonton, Canada
    Surgery 150:48-55. 2011
    ..IDN-6556 (PF3491390) has already been used clinically and can be delivered orally with high portal vein concentrations...
  35. ncbi Preservation of the human pancreas before islet isolation using a two-layer (UW solution-perfluorochemical) cold storage method
    Jonathan R T Lakey
    Department of Surgery, Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1809-11. 2002
  36. doi High prevalence of ovarian cysts in premenopausal women receiving sirolimus and tacrolimus after clinical islet transplantation
    Eman Alfadhli
    Department of Medicine, University of Alberta, Edmonton, AB, Canada
    Transpl Int 22:622-5. 2009
    ..The risk of ovarian cysts should be discussed with female islet transplant candidates and pelvic ultrasounds performed routinely post-transplant...
  37. ncbi Negative and positive co-signaling with anti-BTLA (PJ196) and CTLA4Ig prolongs islet allograft survival
    Wayne Truong
    The Surgical Medical Research Institute, Department of Surgery, Faculty of Medicine, The University of Alberta, Edmonton, Alberta, Canada
    Transplantation 84:1368-72. 2007
    ..The combination of BTLA coinhibitory modulation and CTLA4Ig costimulatory blockade may be an effective adjunctive strategy for inducing long-term allograft survival...
  38. doi Porcine marginal mass islet autografts resist metabolic failure over time and are enhanced by early treatment with liraglutide
    Juliet A Emamaullee
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada T6G 2E1
    Endocrinology 150:2145-52. 2009
    ..These results are discrepant with previous large animal studies and suggest that porcine islets may be resistant to metabolic failure...
  39. ncbi Beta-score: an assessment of beta-cell function after islet transplantation
    Edmond A Ryan
    Heritage Medical Research Centre, Edmonton, Alberta, Canada T6G 2S2
    Diabetes Care 28:343-7. 2005
    ..For the current study, a composite beta-score was developed that provides an integrated measure of beta-cell function success after islet transplantation...
  40. ncbi Strategic opportunities in clinical islet transplantation
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Canada
    Transplantation 79:1304-7. 2005
    ..Future opportunities to expand the donor pool using living donor islet transplantation are within reach, and will be enhanced considerably with both donor and recipient adjunctive treatment using islet-specific growth-factors...
  41. pmc Inhibition of Th17 cells regulates autoimmune diabetes in NOD mice
    Juliet A Emamaullee
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Diabetes 58:1302-11. 2009
    ..The effect of neutralizing anti-IL-17 and recombinant IL-25 on the development of diabetes in NOD mice, a model of spontaneous autoimmune diabetes, was investigated in this study...
  42. doi Caspase inhibitor IDN6556 facilitates marginal mass islet engraftment in a porcine islet autotransplant model
    Michael D McCall
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 94:30-5. 2012
    ..We studied IDN6556, a caspase inhibitor with a first-pass effect, in a large animal (pig) intraportal marginal mass islet autotransplant model...
  43. doi The use of an approved biodegradable polymer scaffold as a solid support system for improvement of islet engraftment
    Tatsuya Kin
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Artif Organs 32:990-3. 2008
    ..These results indicate that biodegradable scaffolds may enhance survival and function of islet grafts. Manipulation of the microenvironment of transplanted islets may constitute the basis for new approaches to enhance islet engraftment...
  44. doi Vascular endothelial growth factor expression in hepatic epithelioid hemangioendothelioma: Implications for treatment and surgical management
    Juliet A Emamaullee
    Department of Surgery, University of Alberta, Edmonton, AB Canada AB T6G 2E1
    Liver Transpl 16:191-7. 2010
    ....
  45. doi Effect of different induction strategies on effector, regulatory and memory lymphocyte sub-populations in clinical islet transplantation
    Christian Toso
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transpl Int 22:182-91. 2009
    ....
  46. ncbi Proteinuria developing after clinical islet transplantation resolves with sirolimus withdrawal and increased tacrolimus dosing
    Peter A Senior
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Am J Transplant 5:2318-23. 2005
    ..Sirolimus should be used cautiously with close monitoring for proteinuria or renal dysfunction...
  47. ncbi Cross-sectional and prospective association between proinsulin secretion and graft function after clinical islet transplantation
    Charlotte G McDonald
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 78:934-7. 2004
    ..TP and PF levels were reduced in transplant recipients compared with controls but increased over time if insulin independence was lost...
  48. ncbi Islet graft assessment in the Edmonton Protocol: implications for predicting long-term clinical outcome
    Cale N Street
    Surgical Medical Research Institute, University of Alberta, Edmonton, Canada
    Diabetes 53:3107-14. 2004
    ..In summary, careful assessment of islet graft composition is needed in a clinical transplantation program to accurately estimate islet purity and assess the contribution of other cell types present, such as islet progenitor cells...
  49. ncbi Compaction of islets is detrimental to transplant outcome in mice
    Shaheed Merani
    Department of Surgery, Faculty of Medicine, University of Alberta, Edmonton AB, Canada
    Transplantation 82:1472-6. 2006
    ..We herein investigate the potential detrimental impact of maintaining islets in a pelleted state for periods preceding implantation. We hypothesize that periods of islet compaction lead to impairment if islet function in vivo...
  50. ncbi Assessment of glycemic control after islet transplantation using the continuous glucose monitor in insulin-independent versus insulin-requiring type 1 diabetes subjects
    Breay W Paty
    Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
    Diabetes Technol Ther 8:165-73. 2006
    ....
  51. ncbi Coronary artery disease is common in nonuremic, asymptomatic type 1 diabetic islet transplant candidates
    Peter A Senior
    Clinical Islet Transplant Program, University of Alberta, 8215 112th St, Edmonton, Alberta, Canada T6G 2C8
    Diabetes Care 28:866-72. 2005
    ..The prevalence of CAD was determined by coronary angiography and the performance of noninvasive cardiac investigation evaluated in type 1 diabetic islet transplant (ITX) candidates with preserved renal function...
  52. doi The role of macrophage migration inhibitory factor in mouse islet transplantation
    Christian Toso
    Islet Transplant Program, Alberta Diabetes Institute, University of Alberta, Edmonton AB, Canada
    Transplantation 86:1361-9. 2008
    ..Macrophage migration inhibitory factor (MIF) is a proinflammatory cytokine produced by many tissues including pancreatic beta-cells...
  53. doi Islets isolated from donors with elevated HbA1c can be successfully transplanted
    Angela Koh
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada
    Transplantation 86:1622-4. 2008
    ..Pancreases from donors with mild hyperglycemia may be a source of islets that could be considered for clinical islet transplantation...
  54. ncbi The portal immunosuppressive storm: relevance to islet transplantation?
    A M James Shapiro
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Ther Drug Monit 27:35-7. 2005
    ..This "portal storm" of immunosuppression may be relevant to intrahepatic islet transplantation...
  55. ncbi Prevalence of hepatic steatosis after islet transplantation and its relation to graft function
    Ravi Bhargava
    Department of Radiology and Diagnostic Imaging, 2A2 42 Walter C Mackenzie Health Sciences Centre, University of Alberta, 8440 112 Street, Edmonton, Alberta T6G 2B7, Canada
    Diabetes 53:1311-7. 2004
    ..001). MRI-detected steatosis is a common finding; the steatosis appears to be due to a paracrine action of insulin secreted from intrahepatic islets. Hepatic steatosis may be associated with insulin resistance or graft dysfunction...
  56. doi Sirolimus-based immunosuppression is associated with increased survival after liver transplantation for hepatocellular carcinoma
    Christian Toso
    Section of Hepatobiliary, Pancreatic and Transplant Surgery, University of Alberta, Edmonton, Canada
    Hepatology 51:1237-43. 2010
    ....
  57. ncbi Histopaque provides optimal mouse islet purification kinetics: comparison study with Ficoll, iodixanol and dextran
    Michael D McCall
    Department of Surgery, University of Alberta, Edmonton, Canada
    Islets 3:144-9. 2011
    ..Using a Ficoll gradient, however, comes at a higher monetary cost. We recommend using a Histopaque gradient, which led to the isolation of viable and functional islets with a reduced cost as compared to a Ficoll gradient...
  58. doi Total tumor volume predicts risk of recurrence following liver transplantation in patients with hepatocellular carcinoma
    Christian Toso
    Section of Hepatobiliary, Pancreatic, and Transplant Surgery, University of Alberta, Edmonton, Canada
    Liver Transpl 14:1107-15. 2008
    ....
  59. ncbi Sirolimus-induced ulceration of the small bowel in islet transplant recipients: report of two cases
    Michele Molinari
    Department of Surgery, Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Am J Transplant 5:2799-804. 2005
    ..Our experience highlights an uncommon but potentially serious adverse effect of high-dose SRL in islet recipients...
  60. ncbi Enhancing the success of human islet isolation through optimization and characterization of pancreas dissociation enzyme
    T Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health, Canada
    Am J Transplant 7:1233-41. 2007
    ..204 was used, suggesting that CII/CI ratio may be a strong predictor to distinguish potential lot success. This study provides a framework for improved enzymatic digestion in human islet isolation...
  61. ncbi Changes in renal function after clinical islet transplantation: four-year observational study
    P A Senior
    Clinical Islet Transplant Program, University of Alberta, Edmonton AB, Canada
    Am J Transplant 7:91-8. 2007
    ..The risk of progressive nephrotoxicity with decline in eGFR should be discussed with prospective CIT candidates and the risk: benefit ratio carefully considered in individuals with pre-existing renal impairment...
  62. ncbi Current indications for pancreas or islet transplant
    Edmond A Ryan
    Department of Medicine, Clinical Islet Transplant Program, University of Alberta and Capital Health, Edmonton, Alberta, Canada
    Diabetes Obes Metab 8:1-7. 2006
    ..In the setting of well-controlled diabetes and intact renal function, it is difficult to justify pancreas or islet transplant alone given the risks of immunosuppression...
  63. ncbi International trial of the Edmonton protocol for islet transplantation
    A M James Shapiro
    Clinical Islet Transplant Program, University of Alberta, Edmonton, AB, Canada
    N Engl J Med 355:1318-30. 2006
    ..We conducted an international, multicenter trial to explore the feasibility and reproducibility of islet transplantation with the use of a single common protocol (the Edmonton protocol)...
  64. pmc Human mesenchymal stem cells protect human islets from pro-inflammatory cytokines
    Telford Y Yeung
    Department of Surgery, 5 002 Li Ka Shing Centre for Health Research Innovation, University of Alberta, Edmonton, Alberta, Canada
    PLoS ONE 7:e38189. 2012
    ..This study, therefore, demonstrates that MSCs may be beneficial for islet engraftment by promoting cell survival and reduced inflammation...
  65. ncbi Intrahepatic islet transplantation in type 1 diabetic patients does not restore hypoglycemic hormonal counterregulation or symptom recognition after insulin independence
    Breay W Paty
    Clinical Islet Transplant Program, University of Alberta, 2000 College Plaza, 8215 112th Street, Edmonton, Alberta, Canada T6G 2C8
    Diabetes 51:3428-34. 2002
    ....
  66. ncbi Assessment of the severity of hypoglycemia and glycemic lability in type 1 diabetic subjects undergoing islet transplantation
    Edmond A Ryan
    Department of Medicine, Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Diabetes 53:955-62. 2004
    ....
  67. ncbi Caspase inhibitor therapy enhances marginal mass islet graft survival and preserves long-term function in islet transplantation
    Juliet A Emamaullee
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Diabetes 56:1289-98. 2007
    ..Our data suggest that caspase inhibitor therapy will reduce the islet mass required in clinical islet transplantation, perhaps to a level that would routinely allow for insulin independence after single-donor infusion...
  68. doi Reassessing selection criteria prior to liver transplantation for hepatocellular carcinoma utilizing the Scientific Registry of Transplant Recipients database
    Christian Toso
    Section of Hepatobiliary, Pancreatic, and Transplant Surgery, University of Alberta, Edmonton, Canada
    Hepatology 49:832-8. 2009
    ..The combined TTV/AFP score efficiently predicted posttransplant survival (hazard ratio = 2, 95% confidence interval = 1.7-2.4, P <or= 0.001); patients not meeting these criteria had a survival below 50% at 3 years...
  69. ncbi XIAP overexpression in human islets prevents early posttransplant apoptosis and reduces the islet mass needed to treat diabetes
    Juliet A Emamaullee
    1 21 Medical Sciences Building, Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2S2, Canada
    Diabetes 54:2541-8. 2005
    ..These results suggest that by transiently overexpressing XIAP in the immediate posttransplant period, human islets from a single donor might be used to effectively treat two diabetic recipients...
  70. doi The impact of sirolimus on hepatocyte proliferation after living donor liver transplantation
    Christian Toso
    Department of Surgery, Section of Hepatobiliary, Pancreatic and Transplant Surgery, University of Alberta Hospital, Edmonton, AB, Canada
    Clin Transplant 24:695-700. 2010
    ..There is a lack of data on the use of sirolimus after partial liver transplantation, especially regarding its impact on post-transplant regeneration...
  71. ncbi Magnetic resonance-defined perinephric edema after clinical islet transplantation: a benign finding associated with mild renal impairment
    Peter A Senior
    Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 78:945-8. 2004
    ..Although this novel observation does not seem to be of concern, further studies are required to examine the long-term impact of CIT and immunosuppression on renal function...
  72. ncbi Advances in pancreatic islet transplantation in humans
    Sulaiman A Nanji
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Diabetes Obes Metab 8:15-25. 2006
    ..This review provides a historical perspective of islet transplantation, outlines the recent advances and current clinical outcomes, and addresses the present challenges and future directions in clinical islet transplantation...
  73. ncbi Percutaneous transhepatic pancreatic islet cell transplantation in type 1 diabetes mellitus: radiologic aspects
    Richard J T Owen
    Departments of Diagnostic Imaging, University of Alberta Hospital, 8440 112th Street, Edmonton, Alberta, Canada T6G 2B7
    Radiology 229:165-70. 2003
    ..To report our experience with percutaneous transhepatic pancreatic islet cell transplantation in patients with type 1 diabetes mellitus...
  74. ncbi Current status of pancreatic islet transplantation
    Shaheed Merani
    Clinical Islet Transplant Program, University of Alberta, Roberts Centre, 2000 College Plaza, Edmonton, Alberta, Canada T6G 2C8
    Clin Sci (Lond) 110:611-25. 2006
    ....
  75. ncbi Combination therapy with sirolimus and interleukin-2 prevents spontaneous and recurrent autoimmune diabetes in NOD mice
    Alex Rabinovitch
    Department of Medicine, University of Alberta, Edmonton, Alberta, Canada
    Diabetes 51:638-45. 2002
    ....
  76. ncbi Successful islet transplantation: continued insulin reserve provides long-term glycemic control
    Edmond A Ryan
    Department of Medicine, Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, 2000 College Plaza, 8215 112th Street, Edmonton, Alberta, Canada T6G 2C8
    Diabetes 51:2148-57. 2002
    ..Islet transplantation is a reasonable option in those with severe problems with glycemic lability or hypoglycemia...
  77. ncbi XIAP overexpression in islet beta-cells enhances engraftment and minimizes hypoxia-reperfusion injury
    Juliet Emamaullee
    Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Canada
    Am J Transplant 5:1297-305. 2005
    ..21 days for Ad-betaGal transduced cells. This approach may allow more efficient use of the limited existing supply of human islets...
  78. ncbi Prevention of bleeding after islet transplantation: lessons learned from a multivariate analysis of 132 cases at a single institution
    P Villiger
    Clinical Islet Transplant Program, University of Alberta
    Am J Transplant 5:2992-8. 2005
    ..02). We conclude that bleeding after percutaneous islet implantation is an avoidable complication provided the intraparenchymal liver tract is sealed effectively...
  79. ncbi Islet transplantation in patients with diabetes mellitus: choice of immunosuppression
    Sulaiman A Nanji
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    BioDrugs 18:315-28. 2004
    ....
  80. ncbi The new deceased donor pancreas allocation schema: do the recommendations go far enough?
    A M James Shapiro
    1Clinical Islet Transplant Program, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 83:1151-2. 2007
  81. ncbi Human islet transplantation from pancreases with prolonged cold ischemia using additional preservation by the two-layer (UW solution/perfluorochemical) cold-storage method
    Toshiaki Tsujimura
    Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 74:1687-91. 2002
    ..In this study, we determined whether the additional preservation by the two-layer method could improve islet recovery from human pancreases with prolonged cold storage in UW...
  82. ncbi Multiple combination therapies involving blockade of ICOS/B7RP-1 costimulation facilitate long-term islet allograft survival
    Sulaiman A Nanji
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Am J Transplant 4:526-36. 2004
    ..These data demonstrate that blockade of the ICOS/B7RP-1 pathway has potential therapeutic benefit given its role in enhancing islet allograft survival and regulating acute alloresponses in vivo...
  83. ncbi Current status of clinical islet cell transplantation
    Jonathan R T Lakey
    Department of Surgery, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada
    Methods Mol Biol 333:47-104. 2006
    ..The challenges facing the clinician-scientist in the 21st century are also presented in this review...
  84. ncbi Effect of core pancreas temperature during cadaveric procurement on human islet isolation and functional viability
    Jonathan R T Lakey
    Department of Surgery and the Surgical Medical Research Institute, University of Alberta, Edmonton, Alberta T6G 2N8, Canada
    Transplantation 73:1106-10. 2002
    ..The aim of this study was to correlate pancreas procurement technique with the core temperature within the pancreas during in situ procurement to the recovery and in vitro function of isolated human islets...
  85. ncbi Interventional strategies to prevent beta-cell apoptosis in islet transplantation
    Juliet A Emamaullee
    Surgical Medical Research Institute, University of Alberta, Edmonton, AB T6G 2N8
    Diabetes 55:1907-14. 2006
    ..In addition, XIAP has enhanced long-term murine islet allograft survival. The complexities of both intrinsic and extrinsic apoptotic pathway inhibition are discussed in depth...
  86. ncbi Detection of microbial contamination during human islet isolation
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Cell Transplant 16:9-13. 2007
    ..Microbial contamination in final products is rare, but de novo contamination still occurs during processing even under cGMP conditions...
  87. ncbi Changes in liver enzymes after clinical islet transplantation
    Ehab Rafael
    Department of Surgery, University of Alberta, Edmonton, Alberta, Canada
    Transplantation 76:1280-4. 2003
    ..The aim of this study was to characterize the pattern of elevation in liver enzymes observed after ITx and to investigate any correlation between these elevations and graft characteristics or graft functional outcome...
  88. ncbi Technical aspects of islet preparation and transplantation
    Jonathan R T Lakey
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Centre, T6G 2N8, Edmonton, Alberta, Canada
    Transpl Int 16:613-32. 2003
    ..This chapter will review the rationale of islet transplantation and the development of islet isolation and purification. The challenges facing clinical islet transplantation in the twenty-first century will also be introduced...
  89. ncbi Risks and side effects of islet transplantation
    Edmond A Ryan
    Clinical Islet Transplant Program, 2000 College Plaza, 8215 112th Street, Edmonton, Alberta T6G 2C8, Canada
    Curr Diab Rep 4:304-9. 2004
    ..For most, improvement in glucose control with resolution of glycemic lability and hypoglycemia has been a net benefit...
  90. ncbi Islet transplants and impact on secondary diabetic complications: does C-Peptide protect the kidney?
    A M James Shapiro
    J Am Soc Nephrol 14:2214-6. 2003
  91. ncbi Impact of recipient age on outcome of ABO-incompatible living-donor liver transplantation
    Hiroto Egawa
    Department of Transplantation Immunology, Faculty of Medicine, Kyoto University, Kyoto, Japan
    Transplantation 77:403-11. 2004
    ..The aim of the present study was to evaluate the outcome of liver transplants performed with ABO-incompatible living-donor livers and to detect risk factors for development of complications...
  92. ncbi Islet transplantation: progress and challenge
    Jason L Gaglia
    Joslin Diabetes Center, Harvard Medical School, Boston, Massachusetts 02215, USA
    Arch Med Res 36:273-80. 2005
    ..In this review, we describe details of the evolution of modern islet transplantation and provide insight into ongoing clinical and basic research efforts to overcome current obstacles for this promising therapy...
  93. ncbi Edmonton's islet success has indeed been replicated elsewhere
    A M James Shapiro
    Lancet 362:1242. 2003
  94. ncbi The TIM family of cosignaling receptors: emerging targets for the regulation of autoimmune disease and transplantation tolerance
    Wayne Truong
    The Surgical Medical Research Institute, Department of Surgery, Faculty of Medicine, University of Alberta, Edmonton, Alberta, Canada
    Cell Transplant 16:977-86. 2008
    ..The current review examines the impact of Tim coreceptor targeting as an emerging therapeutic option for regulating autoimmune diseases and prevention of allograft rejection...
  95. doi Beta-cell transplantation for diabetes therapy
    Camillo Ricordi
    Lancet 372:27-8; author reply 29-30. 2008
  96. ncbi Hepatic artery-portal venous fistula after percutaneous intraportal islet cell transplant
    Amar A Suchak
    Transplantation 83:669-70. 2007
  97. ncbi Pancreas divisum: a study of the cadaveric donor pancreas for islet isolation
    Tatsuya Kin
    Clinical Islet Transplant Program, University of Alberta and Capital Health Authority, Edmonton, Alberta, Canada
    Pancreas 30:325-7. 2005
    ..We investigated the frequency of pancreas divisum and its effect on the digestion process in islet isolation...
  98. ncbi Endogenous pancreatic enzyme activity levels show no significant effect on human islet isolation yield
    Natisha L Rose
    Surgical Medical Research Institute, 1074 Dentistry Pharmacy Centre, University of Alberta, Edmonton, Alberta, T6G 2N8, Canada
    Cell Transplant 13:153-60. 2004
    ..This suggests that further understanding of the isolation process should focus on the role of the collagenase digestion solution in the dissociation of the endocrine-exocrine tissue connection...
  99. ncbi Pancreatic islet autotransplantation with completion pancreatectomy in the management of uncontrolled pancreatic fistula after whipple resection for ampullary adenocarcinoma
    Faisal Alsaif
    Pancreas 32:430-1. 2006
  100. ncbi Follow-up study of the first successful living donor islet transplantation
    Shinichi Matsumoto
    2nd Department of Surgery, Fujita Health University, Aichi, Japan
    Transplantation 82:1629-33. 2006
    ..However, we have very limited number of such donors in Japan; therefore, it is not allowed to perform islet transplantation with brain-dead donors. In order to perform islet transplantation in Japan, we need to seek new donor resources...