K Poole

Summary

Affiliation: Queen's University
Country: Canada

Publications

  1. pmc Interaction of the MexA and MexB components of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa: identification of MexA extragenic suppressors of a T578I mutation in MexB
    Dominic Nehme
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Antimicrob Agents Chemother 49:4375-8. 2005
  2. ncbi Mechanisms of bacterial biocide and antibiotic resistance
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Symp Ser Soc Appl Microbiol . 2002
  3. pmc Protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa
    Denis M Daigle
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 189:5441-51. 2007
  4. ncbi Multidrug resistance in Gram-negative bacteria
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, K7L 3N6, Canada
    Curr Opin Microbiol 4:500-8. 2001
  5. ncbi Multidrug efflux in Pseudomonas aeruginosa: components, mechanisms and clinical significance
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Curr Top Med Chem 1:59-71. 2001
  6. ncbi Mechanisms of bacterial biocide and antibiotic resistance
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Appl Microbiol 92:55S-64S. 2002
  7. ncbi Outer membranes and efflux: the path to multidrug resistance in Gram-negative bacteria
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Curr Pharm Biotechnol 3:77-98. 2002
  8. ncbi Resistance to beta-lactam antibiotics
    K Poole
    Department of Microbiology and Immunology, Queen s University, K7L 3N6, Kingston, Ontario, Canada
    Cell Mol Life Sci 61:2200-23. 2004
  9. ncbi Overcoming multidrug resistance in gram-negative bacteria
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada, K7L 3N6
    Curr Opin Investig Drugs 4:128-39. 2003
  10. ncbi Iron acquisition and its control in Pseudomonas aeruginosa: many roads lead to Rome
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada, K7L 3N6
    Front Biosci 8:d661-86. 2003

Collaborators

Detail Information

Publications69

  1. pmc Interaction of the MexA and MexB components of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa: identification of MexA extragenic suppressors of a T578I mutation in MexB
    Dominic Nehme
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Antimicrob Agents Chemother 49:4375-8. 2005
    ..These data confirm the importance of the MexA C-terminal region in MexB binding and the likely significance of the region surrounding T587I of MexB in MexA interaction...
  2. ncbi Mechanisms of bacterial biocide and antibiotic resistance
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Symp Ser Soc Appl Microbiol . 2002
    ..Issues for consideration include the natural function of these efflux systems and the therapeutic potential of targeting these systems in combating acquired multidrug resistance...
  3. pmc Protein modulator of multidrug efflux gene expression in Pseudomonas aeruginosa
    Denis M Daigle
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 189:5441-51. 2007
    ..Finally, two mutations (L36P and W45A) in ArmR compromising its interaction with MexR have been isolated and mapped to a putative C-terminal alpha-helix of the protein that alone is sufficient for interaction with MexR...
  4. ncbi Multidrug resistance in Gram-negative bacteria
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, K7L 3N6, Canada
    Curr Opin Microbiol 4:500-8. 2001
    ....
  5. ncbi Multidrug efflux in Pseudomonas aeruginosa: components, mechanisms and clinical significance
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Curr Top Med Chem 1:59-71. 2001
    ..While the natural functional of these pumps remains undefined, the fact that they contribute to antimicrobial resistance in P. aeruginosa makes them reasonable targets for therapeutic intervention...
  6. ncbi Mechanisms of bacterial biocide and antibiotic resistance
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Appl Microbiol 92:55S-64S. 2002
    ..Issues for consideration include the natural function of these efflux systems and the therapeutic potential of targeting these systems in combating acquired multidrug resistance...
  7. ncbi Outer membranes and efflux: the path to multidrug resistance in Gram-negative bacteria
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Curr Pharm Biotechnol 3:77-98. 2002
    ..As such, strategies aimed at thwarting efflux and or the outer membrane barrier are effective at reversing antimicrobial resistance in these organisms...
  8. ncbi Resistance to beta-lactam antibiotics
    K Poole
    Department of Microbiology and Immunology, Queen s University, K7L 3N6, Kingston, Ontario, Canada
    Cell Mol Life Sci 61:2200-23. 2004
    ....
  9. ncbi Overcoming multidrug resistance in gram-negative bacteria
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada, K7L 3N6
    Curr Opin Investig Drugs 4:128-39. 2003
    ..Clearly, the antibiotic era has not heralded the defeat of infectious disease and prudent use of novel therapies is imperative if we are to avoid entering the post-antimicrobial era...
  10. ncbi Iron acquisition and its control in Pseudomonas aeruginosa: many roads lead to Rome
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada, K7L 3N6
    Front Biosci 8:d661-86. 2003
    ..aeruginosa may be dissociated in the periplasm, with a common iron carrier then responsible for iron uptake into the cell interior...
  11. pmc Contribution of the MexXY multidrug transporter to aminoglycoside resistance in Pseudomonas aeruginosa clinical isolates
    Mara L Sobel
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Antimicrob Agents Chemother 47:3202-7. 2003
    ..Thus, mexXY hyperexpression in aminoglycoside-resistant clinical isolates occurs via mutation in one or more as yet unidentified genes...
  12. ncbi Efflux-mediated multiresistance in Gram-negative bacteria
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Clin Microbiol Infect 10:12-26. 2004
    ....
  13. pmc Aminoglycoside resistance in Pseudomonas aeruginosa
    Keith Poole
    Department of Microbiology and Immunology, Rm 737 Botterell Hall, Queen s University, Kingston, ON K7L 3N6, Canada
    Antimicrob Agents Chemother 49:479-87. 2005
  14. ncbi Efflux-mediated antimicrobial resistance
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada
    J Antimicrob Chemother 56:20-51. 2005
    ....
  15. ncbi Efflux pumps as antimicrobial resistance mechanisms
    Keith Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada
    Ann Med 39:162-76. 2007
    ....
  16. ncbi Overcoming antimicrobial resistance by targeting resistance mechanisms
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Pharm Pharmacol 53:283-94. 2001
    ..Given the ongoing challenge posed by antimicrobial resistance in bacteria, targeting resistance in this way may be our best hope at prolonging the antibiotic era...
  17. ncbi Overexpression of the mexC-mexD-oprJ efflux operon in nfxB-type multidrug-resistant strains of Pseudomonas aeruginosa
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Mol Microbiol 21:713-24. 1996
    ..These data indicate that the multidrug resistance of nfxB strains is due to overexpression of an efflux operon, mexC-mexD-oprJ, encoding components of a second efflux pump in P. aeruginosa...
  18. pmc Expression of the multidrug resistance operon mexA-mexB-oprM in Pseudomonas aeruginosa: mexR encodes a regulator of operon expression
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Antimicrob Agents Chemother 40:2021-8. 1996
    ..These data suggest that MexR possesses both repressor and activator function in vivo, the activator form being favored in nalB multidrug-resistant strains...
  19. ncbi Multidrug efflux pumps and antimicrobial resistance in Pseudomonas aeruginosa and related organisms
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada
    J Mol Microbiol Biotechnol 3:255-64. 2001
    ....
  20. pmc Cloning and sequence analysis of a gene (pchR) encoding an AraC family activator of pyochelin and ferripyochelin receptor synthesis in Pseudomonas aeruginosa
    D E Heinrichs
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 175:5882-9. 1993
    ..aeruginosa. In agreement with this, a PchR mutant obtained by in vitro mutagenesis and gene replacement was deficient in production of the ferripyochelin receptor and pyochelin...
  21. ncbi The Pseudomonas aeruginosa tonB gene encodes a novel TonB protein
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Microbiology 142:1449-58. 1996
    ..coli tonB strain. The larger size of TonBp.a. and its ability to function in both E. coli and P. putida make it a unique TonB protein whose characterization should enhance our understanding of TonB function in bacteria...
  22. pmc Mutational analysis of the OprM outer membrane component of the MexA-MexB-OprM multidrug efflux system of Pseudomonas aeruginosa
    X Z Li
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 183:12-27. 2001
    ..Koronakis, A. Sharff, E. Koronakis, B. Luisi, and C. Hughes, Nature 405:914-919, 2000). The data are consistent with OprM monomer existing as a substantially periplasmic protein with four outer membrane-spanning regions...
  23. pmc SmeDEF multidrug efflux pump contributes to intrinsic multidrug resistance in Stenotrophomonas maltophilia
    L Zhang
    Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6
    Antimicrob Agents Chemother 45:3497-503. 2001
    ..Apparently, hyperexpressed SmeF is capable of functioning with additional multidrug efflux components to promote multidrug resistance in S. maltophilia...
  24. pmc Multiple antibiotic resistance in Pseudomonas aeruginosa: evidence for involvement of an efflux operon
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 175:7363-72. 1993
    ..These data suggest that ORFA-ORFB-oprK (ORFC)-dependent drug efflux contributes to multiple antibiotic resistance in P. aeruginosa. We propose, therefore, the designation mexAB (multiple efflux) for ORFAB...
  25. ncbi Expression of the ferric enterobactin receptor (PfeA) of Pseudomonas aeruginosa: involvement of a two-component regulatory system
    C R Dean
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Mol Microbiol 8:1095-103. 1993
    ..Analysis of the hydropathy profiles of PfeR and PfeS supported a cytoplasmic location for PfeR and a cytoplasmic membrane location for PfeS...
  26. ncbi Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Mol Microbiol 10:529-44. 1993
    ..Despite the homology of the ORFA and ORFB products to EnvC and EnvD, respectively, the ORFA-ORFB-deficient mutants were not defective in septum formation.(ABSTRACT TRUNCATED AT 400 WORDS)..
  27. pmc Cloning and characterization of the ferric enterobactin receptor gene (pfeA) of Pseudomonas aeruginosa
    C R Dean
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 175:317-24. 1993
    ..The PfeA protein showed more than 60% homology to the E. coli FepA protein. Consistent with this, the two proteins showed significant immunological cross-reactivity...
  28. pmc Inner membrane efflux components are responsible for beta-lactam specificity of multidrug efflux pumps in Pseudomonas aeruginosa
    R Srikumar
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 179:7875-81. 1997
    ..Thus, one or both of inner membrane-associated proteins MexA and MexB are responsible for drug recognition, including recognition of beta-lactams...
  29. pmc Role of the multidrug efflux systems of Pseudomonas aeruginosa in organic solvent tolerance
    X Z Li
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Bacteriol 180:2987-91. 1998
    ..These data suggest that the efflux of organic solvents is a factor in the tolerance of P. aeruginosa to these compounds and that the multidrug efflux systems of this organism can accommodate organic solvents, as well as antibiotics...
  30. pmc MexR repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa: identification of MexR binding sites in the mexA-mexR intergenic region
    K Evans
    Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada, K7L 3N6
    J Bacteriol 183:807-12. 2001
    ....
  31. pmc Cloning and nucleotide sequence analysis of the ferripyoverdine receptor gene fpvA of Pseudomonas aeruginosa
    K Poole
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 175:4597-604. 1993
    ..FpvA also shared strong homology (41.3% identity) with the PupA protein of Pseudomonas putida WCS358. This protein is the receptor for the iron-bound form of pseudobactin, a compound structurally very similar to pyoverdine...
  32. ncbi A second tonB gene in Pseudomonas aeruginosa is linked to the exbB and exbD genes
    Q Zhao
    Department of Microbiology and Immunology, Queen s University, Kingston, Ont, Canada
    FEMS Microbiol Lett 184:127-32. 2000
    ..TonB1 but not TonB2 deletion strains were also compromised as regards the utilization of hemin or hemoglobin as sole iron sources, indicating that heme transport requires TonB1...
  33. pmc The pvc gene cluster of Pseudomonas aeruginosa: role in synthesis of the pyoverdine chromophore and regulation by PtxR and PvdS
    A Stintzi
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 181:4118-24. 1999
    ..pvcABCD expression was negatively regulated by iron and positively regulated by both PvdS, the alternate sigma factor required for pyoverdine biosynthesis, and PtxR, a LysR family activator previously implicated in exotoxin A regulation...
  34. ncbi Fluoroquinolone susceptibilities of efflux-mediated multidrug-resistant Pseudomonas aeruginosa, Stenotrophomonas maltophilia and Burkholderia cepacia
    L Zhang
    Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6
    J Antimicrob Chemother 48:549-52. 2001
    ..The results indicate that these fluoroquinolones are all substrates for the multidrug efflux systems of these organisms. Clinafloxacin was found generally to be the most active agent against multidrug-resistant strains...
  35. pmc Mutations in PA2491 (mexS) promote MexT-dependent mexEF-oprN expression and multidrug resistance in a clinical strain of Pseudomonas aeruginosa
    Mara L Sobel
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 187:1246-53. 2005
    ....
  36. ncbi MexAB-OprM hyperexpression in NalC-type multidrug-resistant Pseudomonas aeruginosa: identification and characterization of the nalC gene encoding a repressor of PA3720-PA3719
    Lily Cao
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada, K7L 3N6
    Mol Microbiol 53:1423-36. 2004
    ..The deduced products of PA3720-PA3719 show no homology to sequences presently in the GenBank databases, however, and as such provide no clues as to how this might occur...
  37. pmc Mutations in PA3574 (nalD) lead to increased MexAB-OprM expression and multidrug resistance in laboratory and clinical isolates of Pseudomonas aeruginosa
    Mara L Sobel
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Antimicrob Agents Chemother 49:1782-6. 2005
    ....
  38. pmc nalD encodes a second repressor of the mexAB-oprM multidrug efflux operon of Pseudomonas aeruginosa
    Yuji Morita
    Department of Microbiology and Immunology, Queen s University, Kingston, ON, Canada K7L 3N6
    J Bacteriol 188:8649-54. 2006
    ..Moreover, increased expression from this promoter was seen in a nalD mutant, consistent with NalD directly controlling mexAB-oprM expression from a second promoter...
  39. pmc MexCD-OprJ multidrug efflux system of Pseudomonas aeruginosa: involvement in chlorhexidine resistance and induction by membrane-damaging agents dependent upon the AlgU stress response sigma factor
    Sebastien Fraud
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L3N6
    Antimicrob Agents Chemother 52:4478-82. 2008
    ..Hyperexpression of this efflux system in nfxB mutants was also substantially AlgU dependent. CHX resistance correlated with efflux gene expression in various mutants, consistent with MexCD-OprJ being a determinant of CHX resistance...
  40. pmc Differential impact of MexB mutations on substrate selectivity of the MexAB-OprM multidrug efflux pump of Pseudomonas aeruginosa
    Jocelyn K Middlemiss
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 186:1258-69. 2004
    ....
  41. pmc The mexR repressor of the mexAB-oprM multidrug efflux operon in Pseudomonas aeruginosa: characterization of mutations compromising activity
    Lateef Adewoye
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 184:4308-12. 2002
    ..Changes at residues L95 and R21, however, produced a stable MexR protein capable of dimerization and, thus, likely compromised DNA binding...
  42. pmc Antibiotic inducibility of the MexXY multidrug efflux system of Pseudomonas aeruginosa: involvement of the antibiotic-inducible PA5471 gene product
    Yuji Morita
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 188:1847-55. 2006
    ....
  43. pmc FpvIR control of fpvA ferric pyoverdine receptor gene expression in Pseudomonas aeruginosa: demonstration of an interaction between FpvI and FpvR and identification of mutations in each compromising this interaction
    Gyula Alan Rédly
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 187:5648-57. 2005
    ....
  44. pmc SmeC, an outer membrane multidrug efflux protein of Stenotrophomonas maltophilia
    Xian Zhi Li
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Antimicrob Agents Chemother 46:333-43. 2002
    ..This represents the first report of coregulation of an efflux resistance determinant and a beta-lactamase...
  45. pmc Oxidative stress induction of the MexXY multidrug efflux genes and promotion of aminoglycoside resistance development in Pseudomonas aeruginosa
    Sebastien Fraud
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L3N6
    Antimicrob Agents Chemother 55:1068-74. 2011
    ..Moreover, PA5471 was required for mexXY expression and aminoglycoside resistance in these as well as several CF isolates examined...
  46. pmc Assembly of the MexAB-OprM multidrug pump of Pseudomonas aeruginosa: component interactions defined by the study of pump mutant suppressors
    Dominic Nehme
    Department of Microbiology and Immunology, Queen s University, Botterell Hall, Room 737, Kingston K7L 3N6, Canada
    J Bacteriol 189:6118-27. 2007
    ..These data highlight the importance of MexA and interactions with this adapter in promoting MexAB-OprM pump assembly and in stabilizing the pump complex...
  47. pmc mexEF-oprN multidrug efflux operon of Pseudomonas aeruginosa: regulation by the MexT activator in response to nitrosative stress and chloramphenicol
    Hossam Fetar
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    Antimicrob Agents Chemother 55:508-14. 2011
    ....
  48. doi Citrate-mediated iron uptake in Pseudomonas aeruginosa: involvement of the citrate-inducible FecA receptor and the FeoB ferrous iron transporter
    Bryan Marshall
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    Microbiology 155:305-15. 2009
    ..These data indicate that Fe delivered to P. aeruginosa by citrate is released as Fe2+, probably in the periplasm, prior to its transport into cells via Fe transport components...
  49. pmc Pyoverdine-mediated regulation of FpvA synthesis in Pseudomonas aeruginosa: involvement of a probable extracytoplasmic-function sigma factor, FpvI
    Gyula Alan Rédly
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 185:1261-5. 2003
    ..A Fur-binding site was identified immediately upstream of fpvI, consistent with the observed iron-regulated expression of fpvI and fpvA...
  50. pmc Mutational analysis of the TonB1 energy coupler of Pseudomonas aeruginosa
    Qixun Zhao
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Bacteriol 184:1503-13. 2002
    ..aeruginosa, although chimeras possessing an E. coli C terminus were active in E. coli...
  51. pmc FpvA-mediated ferric pyoverdine uptake in Pseudomonas aeruginosa: identification of aromatic residues in FpvA implicated in ferric pyoverdine binding and transport
    Jiang Sheng Shen
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Bacteriol 187:8511-5. 2005
    ..Alanine substitutions in three of these, W362, W391, and F795, markedly compromised ferric pyoverdine binding and transport, consistent with a role of FpvA in ferric pyoverdine recognition...
  52. pmc Translational control of the antibiotic inducibility of the PA5471 gene required for mexXY multidrug efflux gene expression in Pseudomonas aeruginosa
    Yuji Morita
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada
    J Bacteriol 191:4966-75. 2009
    ..aeruginosa. These data suggest that PA5471 is not sufficient for MexXY recruitment in response to antibiotic exposure and that additional antibiotic-dependent effects are needed...
  53. pmc Differential effects of mutations in tonB1 on intrinsic multidrug resistance and iron acquisition in Pseudomonas aeruginosa
    Qixun Zhao
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 184:2045-9. 2002
    ....
  54. pmc Assembly of the MexAB-OprM multidrug efflux system of Pseudomonas aeruginosa: identification and characterization of mutations in mexA compromising MexA multimerization and interaction with MexB
    Dominic Nehme
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario K7L 3N6, Canada
    J Bacteriol 186:2973-83. 2004
    ..Still, all but one of the MexA mutations studied compromised MexA-MexB association, suggesting that native structure and/or proper assembly of the protein may be necessary for this...
  55. pmc FpvA receptor involvement in pyoverdine biosynthesis in Pseudomonas aeruginosa
    Jiangsheng Shen
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Bacteriol 184:3268-75. 2002
    ..These data are consistent with the idea that the N-terminal extension plays a role in FpvA-mediated pyoverdine biosynthesis in P. aeruginosa...
  56. ncbi Role of the acetyltransferase AAC(6')-Iz modifying enzyme in aminoglycoside resistance in Stenotrophomonas maltophilia
    Xian Zhi Li
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    J Antimicrob Chemother 51:803-11. 2003
    ..These results indicate that the aac(6')-Iz gene is an important contributor to aminoglycoside resistance in clinical strains of S. maltophilia, particularly to tobramycin...
  57. pmc Role of phosphoglucomutase of Stenotrophomonas maltophilia in lipopolysaccharide biosynthesis, virulence, and antibiotic resistance
    Geoffrey A McKay
    Department of Microbiology and Immunology, Queen s University, Kingston, Ontario, Canada K7L 3N6
    Infect Immun 71:3068-75. 2003
    ..These data highlight the contribution made by LPS to the antimicrobial resistance and virulence of S. maltophilia...
  58. pmc Influence of quorum sensing and iron on twitching motility and biofilm formation in Pseudomonas aeruginosa
    Glenn M Patriquin
    Queen s University, Department of Microbiology and Immunology, Rm 737, Botterell Hall, Kingston, Ontario, Canada
    J Bacteriol 190:662-71. 2008
    ....
  59. ncbi Crystal structure of the MexR repressor of the mexRAB-oprM multidrug efflux operon of Pseudomonas aeruginosa
    Daniel Lim
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada
    J Biol Chem 277:29253-9. 2002
    ..Our results indicate that MexR may differ from the other described member of this family, MarR, in the nature of its effector, mode of DNA binding, and mechanism of regulation...
  60. pmc Multidrug efflux systems play an important role in the invasiveness of Pseudomonas aeruginosa
    Yoichi Hirakata
    Division of Infectious and Immunological Diseases, Department of Pediatrics, University of British Columbia, Vancouver, British Columbia, V5Z 4H4 Canada
    J Exp Med 196:109-18. 2002
    ..We conclude that the P. aeruginosa MexAB-OprM efflux system exports virulence determinants that contribute to bacterial virulence...
  61. pmc Contributions of MexAB-OprM and an EmrE homolog to intrinsic resistance of Pseudomonas aeruginosa to aminoglycosides and dyes
    Xian Zhi Li
    Department of Molecular and Cell Biology, University of California, Berkeley, California 94720 3206, USA
    Antimicrob Agents Chemother 47:27-33. 2003
    ..The EmrE-dependent efflux of ethidium bromide was confirmed by a continuous fluorescence assay...
  62. pmc The crystal structure of MexR from Pseudomonas aeruginosa in complex with its antirepressor ArmR
    Mark S Wilke
    Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada V6T1Z3
    Proc Natl Acad Sci U S A 105:14832-7. 2008
    ..Thus, this work defines the structural mechanism by which ArmR allosterically derepresses MexR-controlled gene expression in P. aeruginosa and reveals important insights into the regulation of multidrug resistance...
  63. ncbi Mathematical modelling response of Pseudomonas aeruginosa to meropenem
    Vincent H Tam
    University of Houston College of Pharmacy, 1441 Moursund Street, Houston, TX 77030, USA
    J Antimicrob Chemother 60:1302-9. 2007
    ..We explored the use of mathematical modelling to guide selection of dosing regimens...
  64. ncbi Prevalence, mechanisms, and risk factors of carbapenem resistance in bloodstream isolates of Pseudomonas aeruginosa
    Vincent H Tam
    Department of Clinical Sciences and Administration, University of Houston College of Pharmacy, Houston, TX 77030, USA
    Diagn Microbiol Infect Dis 58:309-14. 2007
    ..aeruginosa. Understanding the prevalence and mechanism of carbapenem resistance in P. aeruginosa may guide empiric therapy for nosocomial infections in our hospital...
  65. ncbi Uninhibited antibiotic target discovery via chemical genetics
    Keith Poole
    Nat Biotechnol 22:1528-9. 2004
  66. ncbi Binding properties of pyochelin and structurally related molecules to FptA of Pseudomonas aeruginosa
    Gaëtan L A Mislin
    Métaux et Microorganismes Chimie, Biologie et Applications, UMR 7175 LC1 Institut Gilbert Laustriat, CNRS, Université Louis Pasteur Strasbourg 1, ESBS, Bld Sébastien Brant, F 67413 Illkirch, Strasbourg, France
    J Mol Biol 357:1437-48. 2006
    ..All these findings improve our understanding of specificity of the interaction between FptA and its siderophore...
  67. pmc Induction of the MexXY efflux pump in Pseudomonas aeruginosa is dependent on drug-ribosome interaction
    Katy Jeannot
    Laboratoire de Bacteriologie, Hopital Jean Minjoz, 25030 Besancon Cedex, France
    J Bacteriol 187:5341-6. 2005
    ..Altogether, these data demonstrate physiological interplays between MexXY and the ribosome and are suggestive of an alternative function for MexXY beyond antibiotic efflux...
  68. pmc Optimization of meropenem minimum concentration/MIC ratio to suppress in vitro resistance of Pseudomonas aeruginosa
    Vincent H Tam
    University of Houston College of Pharmacy, 1441 Moursund Street, Houston, TX 77030, USA
    Antimicrob Agents Chemother 49:4920-7. 2005
    ..aeruginosa resistance. Our in vitro data support the use of an extended duration of meropenem infusion for the treatment of severe nosocomial infections in combination with an aminoglycoside...
  69. ncbi Formation of the chromophore of the pyoverdine siderophores by an oxidative cascade
    Pieter C Dorrestein
    Department of Chemistry and Chemical Biology, 120 Baker Laboratory, Cornell University, Ithaca, NY 14853, USA
    Org Lett 5:2215-7. 2003
    ..This oxidative cascade can be carried out with polyphenol oxidase (PPO), MnO(2), and cell-free extracts from Pseudomonas aeruginosa. [reaction: see text]..