Sean W Kennedy

Summary

Affiliation: Environment Canada
Country: Canada

Publications

  1. ncbi request reprint Hepatic EROD activity is not a useful biomarker of polychlorinated biphenyl exposure in the adult herring gull (Larus argentatus)
    Sean W Kennedy
    Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottawa, Ontario, Canada K1A 0H3
    Ecotoxicology 12:153-61. 2003
  2. ncbi request reprint Sensitivity of bald eagle (Haliaeetus leucocephalus) hepatocyte cultures to induction of cytochrome P4501A by 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Sean W Kennedy
    Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottowa, Ontario, Canada K1A 0H3
    Ecotoxicology 12:163-70. 2003
  3. doi request reprint Pipping success and liver mRNA expression in chicken embryos exposed in ovo to C8 and C11 perfluorinated carboxylic acids and C10 perfluorinated sulfonate
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario K1N 6N5, Canada
    Toxicol Lett 190:134-9. 2009
  4. doi request reprint A luciferase reporter gene assay and aryl hydrocarbon receptor 1 genotype predict the LD50 of polychlorinated biphenyls in avian species
    Gillian E Manning
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    Toxicol Appl Pharmacol 263:390-401. 2012
  5. doi request reprint Induction of cytochrome P4501A by highly purified hexachlorobenzene in primary cultures of ring-necked pheasant and Japanese quail embryo hepatocytes
    Lukas J Mundy
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 155:498-505. 2012
  6. doi request reprint In vitro and in ovo effects of four brominated flame retardants on toxicity and hepatic mRNA expression in chicken embryos
    Caroline Egloff
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario, Canada K1N6N5
    Toxicol Lett 207:25-33. 2011
  7. doi request reprint Cytochrome P4501A induction in avian hepatocyte cultures exposed to polychlorinated biphenyls: comparisons with AHR1-mediated reporter gene activity and in ovo toxicity
    Gillian E Manning
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    Toxicol Appl Pharmacol 266:38-47. 2013
  8. doi request reprint Cytochrome P4501A induction in primary cultures of embryonic European starling hepatocytes exposed to TCDD, PeCDF and TCDF
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics Department of Biology, University of Ottawa, Ottawa, ON, K1N 6N5, Canada
    Ecotoxicology 22:731-9. 2013
  9. doi request reprint In ovo effects of perfluorohexane sulfonate and perfluorohexanoate on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryos
    Cristina G Cassone
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 127:216-24. 2012
  10. doi request reprint In Ovo effects of two organophosphate flame retardants--TCPP and TDCPP--on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryos
    Amani Farhat
    Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Toxicol Sci 134:92-102. 2013

Collaborators

Detail Information

Publications60

  1. ncbi request reprint Hepatic EROD activity is not a useful biomarker of polychlorinated biphenyl exposure in the adult herring gull (Larus argentatus)
    Sean W Kennedy
    Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottawa, Ontario, Canada K1A 0H3
    Ecotoxicology 12:153-61. 2003
    ..CYP1A protein concentration was significantly correlated with total PCB concentration, but the linear relationship had little predictive power. We conclude that EROD is not a useful biomarker of PCB exposure in the adult herring gull...
  2. ncbi request reprint Sensitivity of bald eagle (Haliaeetus leucocephalus) hepatocyte cultures to induction of cytochrome P4501A by 2,3,7,8-tetrachlorodibenzo-p-dioxin
    Sean W Kennedy
    Environment Canada, Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottowa, Ontario, Canada K1A 0H3
    Ecotoxicology 12:163-70. 2003
    ..This new method is used routinely in our laboratory to prepare hepatocyte cultures from birds for examination of various biochemical responses to environmental contaminants...
  3. doi request reprint Pipping success and liver mRNA expression in chicken embryos exposed in ovo to C8 and C11 perfluorinated carboxylic acids and C10 perfluorinated sulfonate
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario K1N 6N5, Canada
    Toxicol Lett 190:134-9. 2009
    ..Based on the present results for white leghorn chickens, current environmental concentrations of PFOA, PFUdA and PFDS are unlikely to affect the hatching success of wild birds...
  4. doi request reprint A luciferase reporter gene assay and aryl hydrocarbon receptor 1 genotype predict the LD50 of polychlorinated biphenyls in avian species
    Gillian E Manning
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    Toxicol Appl Pharmacol 263:390-401. 2012
    ....
  5. doi request reprint Induction of cytochrome P4501A by highly purified hexachlorobenzene in primary cultures of ring-necked pheasant and Japanese quail embryo hepatocytes
    Lukas J Mundy
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 155:498-505. 2012
    ....
  6. doi request reprint In vitro and in ovo effects of four brominated flame retardants on toxicity and hepatic mRNA expression in chicken embryos
    Caroline Egloff
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie Street, Ottawa, Ontario, Canada K1N6N5
    Toxicol Lett 207:25-33. 2011
    ..None of the gene targets were responsive to BEHTBP exposure in CEH. The multi-tiered in vitro/in ovo screening approach was effective for assessing toxicological and molecular biological effects of these BFRs in an avian species...
  7. doi request reprint Cytochrome P4501A induction in avian hepatocyte cultures exposed to polychlorinated biphenyls: comparisons with AHR1-mediated reporter gene activity and in ovo toxicity
    Gillian E Manning
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    Toxicol Appl Pharmacol 266:38-47. 2013
    ..Relative potency values were generally similar between the LRG and EROD assays and indicate that the relative potency of some PCBs may differ among species...
  8. doi request reprint Cytochrome P4501A induction in primary cultures of embryonic European starling hepatocytes exposed to TCDD, PeCDF and TCDF
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics Department of Biology, University of Ottawa, Ottawa, ON, K1N 6N5, Canada
    Ecotoxicology 22:731-9. 2013
    ..However, starling hepatocytes are less sensitive than chicken hepatocytes to CYP1A4/5 induction by PeCDF and TCDF...
  9. doi request reprint In ovo effects of perfluorohexane sulfonate and perfluorohexanoate on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryos
    Cristina G Cassone
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 127:216-24. 2012
    ..This in ovo study successfully validated previous in vitro results concerning the modulation of TH-responsive genes and identified adverse effects associated with TH homeostasis in response to PFHxS treatment...
  10. doi request reprint In Ovo effects of two organophosphate flame retardants--TCPP and TDCPP--on pipping success, development, mRNA expression, and thyroid hormone levels in chicken embryos
    Amani Farhat
    Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Toxicol Sci 134:92-102. 2013
    ..The effects of TDCPP on the gallbladder indicate that the disturbance of lipid metabolism is a likely mechanism of toxicity. ..
  11. doi request reprint Tris(1,3-dichloro-2-propyl) phosphate perturbs the expression of genes involved in immune response and lipid and steroid metabolism in chicken embryos
    Amani Farhat
    Department of Biology, University of Ottawa, Ottawa, ON K1N 6N5, Canada National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3, Canada
    Toxicol Appl Pharmacol 275:104-12. 2014
    ..Finally, our results are in concordance with the literature that describes TDCPP as a cancer-causing agent, since the majority of dysregulated genes were involved in cancer pathways...
  12. doi request reprint Amino acid sequence of the ligand-binding domain of the aryl hydrocarbon receptor 1 predicts sensitivity of wild birds to effects of dioxin-like compounds
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    Toxicol Sci 131:139-52. 2013
    ..93, p < 0.0001) with in ovo toxicity data for those species. These results indicate promise for the use of AHR1 LBD amino acid sequences independently, or combined with the LRG assay, to predict avian species sensitivity to DLCs...
  13. doi request reprint 1,2-Dibromo-4-(1,2-dibromoethyl)-cyclohexane and tris(methylphenyl) phosphate cause significant effects on development, mRNA expression, and circulating bile acid concentrations in chicken embryos
    Doug Crump
    National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3, Canada Electronic address
    Toxicol Appl Pharmacol 277:279-87. 2014
    ....
  14. doi request reprint Pipping success, isomer-specific accumulation, and hepatic mRNA expression in chicken embryos exposed to HBCD
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 115:492-500. 2010
    ..Research on the effects of HBCD in wild avian species is warranted...
  15. doi request reprint Sequence and in vitro function of chicken, ring-necked pheasant, and Japanese quail AHR1 predict in vivo sensitivity to dioxins
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    Environ Sci Technol 46:2967-75. 2012
    ..Our results support and expand previous in vitro and in vivo work that demonstrated ligand-dependent species differences in AHR1 affinity. The findings and methods will be of use for DLC risk assessments...
  16. doi request reprint In vitro microarray analysis identifies genes in acute-phase response pathways that are down-regulated in the liver of chicken embryos exposed in ovo to PFUdA
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, 20 Marie Curie, Ottawa, ON, Canada
    Toxicol In Vitro 27:1649-58. 2013
    ..These findings suggest in ovo exposure of birds to PFUdA may lead to post-hatch developmental deficiencies, such as impaired inflammatory response...
  17. doi request reprint Chicken embryo cardiomyocyte cultures--a new approach for studying effects of halogenated aromatic hydrocarbons in the avian heart
    Stephanie P Jones
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada
    Toxicol Sci 109:66-74. 2009
    ..Induction of CYP1A5 by TCDD in avian cardiomyocytes is a novel finding, and might help direct future studies on mechanisms of action of HAHs in the heart...
  18. doi request reprint Technical-grade perfluorooctane sulfonate alters the expression of more transcripts in cultured chicken embryonic hepatocytes than linear perfluorooctane sulfonate
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Environ Toxicol Chem 30:2846-59. 2011
    ..These data show that T-PFOS elicits a greater transcriptional response in CEH than L-PFOS alone and demonstrates the importance of considering the isomer-specific toxicological properties of PFOS when assessing exposure risk...
  19. doi request reprint Effects of co-exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin and perfluorooctane sulfonate or perfluorooctanoic acid on expression of cytochrome P450 isoforms in chicken (Gallus gallus) embryo hepatocyte cultures
    Michio X Watanabe
    National Wildlife Research Centre, Environment Canada, Carleton University, Ottawa, ON, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 149:605-12. 2009
    ..Our data suggest a complex gene response to mixtures of dioxin-like and perfluorinated compounds...
  20. doi request reprint Use of an avian hepatocyte assay and the avian Toxchip Polymerse chain reaction array for testing prioritization of 16 organic flame retardants
    Emily Porter
    National Wildlife Research Centre, Environment Canada, Ottawa, Ontario, Canada
    Environ Toxicol Chem 33:573-82. 2014
    ....
  21. doi request reprint 2,3,4,7,8-pentachlorodibenzofuran is a more potent cytochrome P4501A inducer than 2,3,7,8-tetrachlorodibenzo-p-dioxin in herring gull hepatocyte cultures
    Jessica C Hervé
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    Environ Toxicol Chem 29:2088-95. 2010
    ....
  22. pmc Effects of perfluoroalkyl compounds on mRNA expression levels of thyroid hormone-responsive genes in primary cultures of avian neuronal cells
    Viengtha Vongphachan
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    Toxicol Sci 120:392-402. 2011
    ..This is the first study to report that PFC exposure alters mRNA expression in primary cultures of avian neuronal cells and may provide insight into the possible mechanisms of action of PFCs in the avian brain...
  23. ncbi request reprint Same-sample analysis of ethoxyresorufin-O-deethylase activity and cytochrome P4501A mRNA abundance in chicken embryo hepatocytes
    Jessica A Head
    Center for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ont, Canada K1N 6N5
    Anal Biochem 360:294-302. 2007
    ..When working with hepatocytes cultured from wild or endangered species, our approach can help to circumvent the problem of small sample size by maximizing the amount of data obtained from each sample...
  24. doi request reprint Effects of tris(1,3-dichloro-2-propyl) phosphate and tris(1-chloropropyl) phosphate on cytotoxicity and mRNA expression in primary cultures of avian hepatocytes and neuronal cells
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 126:140-8. 2012
    ..The mRNA expression data were similar to those from a previous study with hexabromocyclododecane...
  25. doi request reprint Highly purified hexachlorobenzene induces cytochrome P4501A in primary cultures of chicken embryo hepatocytes
    Lukas J Mundy
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Toxicol Appl Pharmacol 248:185-93. 2010
    ..The maximal EROD activity and CYP1A4/5 mRNA expression differed greatly between HCB and TCDD, and may contribute to an overestimation of the ReP value calculated for highly purified HCB...
  26. doi request reprint Cytochrome P4501A induction by 2,3,7,8-tetrachlorodibenzo-p-dioxin and two chlorinated dibenzofurans in primary hepatocyte cultures of three avian species
    Jessica C Hervé
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ontario, Canada
    Toxicol Sci 113:380-91. 2010
    ..This study indicates that PeCDF is more potent than TCDD in ring-necked pheasant and Japanese quail hepatocytes and that ring-necked pheasant, Japanese quail, and chicken hepatocytes are equally sensitive to CYP1A induction by PeCDF...
  27. doi request reprint Exposure to perfluorooctane sulfonate or fenofibrate causes PPAR-alpha dependent transcriptional responses in chicken embryo hepatocytes
    Matthew A Cwinn
    Department of Biology and Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, ON, Canada K1N 6N5
    Comp Biochem Physiol C Toxicol Pharmacol 148:165-71. 2008
    ....
  28. doi request reprint Transcriptional profiles in the cerebral hemisphere of chicken embryos following in ovo perfluorohexane sulfonate exposure
    Cristina G Cassone
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada K1A 0H3
    Toxicol Sci 129:380-91. 2012
    ....
  29. doi request reprint Effects of hexabromocyclododecane and polybrominated diphenyl ethers on mRNA expression in chicken (Gallus domesticus) hepatocytes
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada
    Toxicol Sci 106:479-87. 2008
    ....
  30. doi request reprint Isomer-specific accumulation of perfluorooctane sulfonate in the liver of chicken embryos exposed in ovo to a technical mixture
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ontario, Canada
    Environ Toxicol Chem 30:226-31. 2011
    ..This supports the hypothesis that the pharmacokinetics of PFOS are isomer-specific in biota, and may help explain why wildlife PFOS burdens are dominated by L-PFOS relative to T-PFOS mixtures...
  31. doi request reprint Species-specific relative AHR1 binding affinities of 2,3,4,7,8-pentachlorodibenzofuran explain avian species differences in its relative potency
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada Environment Canada, National Wildlife Research Centre, Ottawa, Ontario K1A 0H3, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 161:21-5. 2014
    ..The current report introduces a COS-7 whole-cell binding assay and provides a mechanistic explanation for differential relative potencies of PeCDF among species of birds. ..
  32. doi request reprint The effects of Dechlorane Plus on toxicity and mRNA expression in chicken embryos: a comparison of in vitro and in ovo approaches
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 154:129-34. 2011
    ..At current environmental exposure levels, no adverse effects of DP on embryonic viability or pathways associated with the genes assessed are predicted...
  33. ncbi request reprint Detection of polybrominated diphenyl ethers in herring gull (Larus argentatus) brains: effects on mRNA expression in cultured neuronal cells
    Doug Crump
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario, Canada
    Environ Sci Technol 42:7715-21. 2008
    ..Herring gull neuronal cells were responsive to both T3 and PBDEs although, receptors associated with two predicted mechanisms of PBDE action were not effective molecular biomarkers of exposure...
  34. ncbi request reprint Health of herring gulls (Larus argentatus) in relation to breeding location in the early 1990s. I. Biochemical measures
    Glen A Fox
    Canadian Wildlife Service, Environment Canada, National Wildlife Research Centre, Carleton University, Ottawa, Ontario, Canada
    J Toxicol Environ Health A 70:1443-70. 2007
    ..The health of adult herring gulls varied with breeding location and "lifestyle" in the early 1990s, and Great Lakes gulls suffered from chemical and nutritional stressors that modulated physiological processes and endocrine function...
  35. doi request reprint Tris(2-butoxyethyl)phosphate and triethyl phosphate alter embryonic development, hepatic mRNA expression, thyroid hormone levels, and circulating bile acid concentrations in chicken embryos
    Caroline Egloff
    National Wildlife Research Centre, Environment Canada, Ottawa, ON K1A 0H3, Canada
    Toxicol Appl Pharmacol 279:303-10. 2014
    ..The increase in plasma bile acids and LSI, gallbladder hypotrophy, and discoloration of liver tissue represented potential phenotypic outcomes associated with modulation of hepatic genes involved with xenobiotic and lipid metabolism...
  36. doi request reprint Time-dependent effects of the flame retardant tris(1,3-dichloro-2-propyl) phosphate (TDCPP) on mRNA expression, in vitro and in ovo, reveal optimal sampling times for rapidly metabolized compounds
    Amani Farhat
    National Wildlife Research Centre, Environment Canada, Ottawa, Ontario, Canada
    Environ Toxicol Chem 33:2842-9. 2014
    ..Overall, TDCPP elicits the transcriptional and phenotypic alterations observed in vitro and in ovo, whereas its major metabolite, BDCPP, is far less biologically active...
  37. doi request reprint Perfluorooctane sulfonate (PFOS) toxicity in domestic chicken (Gallus gallus domesticus) embryos in the absence of effects on peroxisome proliferator activated receptor alpha (PPARalpha)-regulated genes
    Jason M O'Brien
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 149:524-30. 2009
    ..PFOS exposure via egg injection prior to incubation did not affect the transcriptional activity of any of the assayed PPARalpha-regulated genes at any of the doses examined in day 21 chicken embryos...
  38. ncbi request reprint Fluorescent RNA arbitrarily primed polymerase chain reaction. A new differential display approach to detect contaminant-induced alterations of gene expression in wildlife species
    Doug Crump
    National Wildlife Research Center, Canadian Wildlife Service, Carleton University, Ottawa, Ontario, Canada
    Methods Mol Biol 410:15-27. 2008
    ..g., cultured cells, tissue). This straightforward, safe, and cost-effective approach represents a useful alternative to the radiometric-based RAP-PCR method...
  39. ncbi request reprint Exposure to 3,3',4,4',5-pentachlorobiphenyl during embryonic development has a minimal effect on the cytochrome P4501A response to 2,3,7,8-tetrachlorodibenzo-p-dioxin in cultured chicken embryo hepatocytes
    Jessica A Head
    Center for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada
    Environ Toxicol Chem 25:2981-9. 2006
    ..These findings suggest that exposure to environmental levels of dioxin-like compounds during incubation is not likely to alter species-sensitivity estimates derived from in vitro CYP1A data...
  40. doi request reprint Sunlight Irradiation of Highly Brominated Polyphenyl Ethers Generates Polybenzofuran Products That Alter Dioxin-responsive mRNA Expression in Chicken Hepatocytes
    Guanyong Su
    Ecotoxicology and Wildlife Health Division, Environment and Climate Change Canada, National Wildlife Research Centre, Carleton University, Ottawa, Ontario K1A 0H3, Canada
    Environ Sci Technol 50:2318-27. 2016
    ..Our findings clearly show that the environmental stability of TeDB-DiPhOBz and BDE-209, and possibly other highly brominated polyphenyl ethers, is of great concern from a dioxin-like degradation products and toxicity perspective. ..
  41. doi request reprint Ethoxyresorufin O-deethylase induction by TCDD, PeCDF and TCDF in ring-necked pheasant and Japanese quail hepatocytes: Time-dependent effects on concentration-response curves
    Jessica C Hervé
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5
    Toxicol In Vitro 24:1301-5. 2010
    ....
  42. doi request reprint Effects of 18 perfluoroalkyl compounds on mRNA expression in chicken embryo hepatocyte cultures
    Nathan J Hickey
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario K1A 0H3, Canada
    Toxicol Sci 111:311-20. 2009
    ..This study is the first to expose CEH cultures to multiple PFCs in vitro and demonstrates that exposure to PFC solutions of different isomeric content or chain length causes variable transcriptional responses...
  43. ncbi request reprint Differential expression, induction, and stability of CYP1A4 and CYP1A5 mRNA in chicken and herring gull embryo hepatocytes
    Jessica A Head
    Center for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario, Canada
    Comp Biochem Physiol C Toxicol Pharmacol 145:617-24. 2007
    ..We propose that CYP1A5 mRNA expression may be a sensitive biomarker of exposure to dioxin-like compounds in some avian species...
  44. doi request reprint Time-dependent transcriptomic and biochemical responses of 6-formylindolo[3,2-b]carbazole (FICZ) and 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) are explained by AHR activation time
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada Environment and Climate Change Canada, National Wildlife Research Centre, Ottawa, Ontario K1A 0H3, Canada Electronic address
    Biochem Pharmacol 115:134-43. 2016
    ..Moreover, rapid metabolism of FICZ in hepatocytes resulted in a significant decrease in the induction of EROD activity. ..
  45. doi request reprint Biochemical and Transcriptomic Effects of Herring Gull Egg Extracts from Variably Contaminated Colonies of the Laurentian Great Lakes in Chicken Hepatocytes
    Doug Crump
    Ecotoxicology and Wildlife Health Division, Environment Canada, National Wildlife Research Centre, Carleton University, Ottawa, ON, Canada K1A 0H3
    Environ Sci Technol 49:10190-8. 2015
    ..Such high-throughput assays show potential promise as cost-effective tools for determining toxic potencies of complex mixtures in the environment...
  46. doi request reprint Photolytic degradation products of two highly brominated flame retardants cause cytotoxicity and mRNA expression alterations in chicken embryonic hepatocytes
    Guanyong Su
    Ecotoxicology and Wildlife Health Division, Environment Canada, National Wildlife Research Centre, Carleton University, Ottawa, Ontario K1A 0H3, Canada
    Environ Sci Technol 48:12039-46. 2014
    ..Overall, degradation of TeDB-DiPhOBz and BDE-209 by natural sunlight generates byproducts that affect in vitro expression of genes, especially the AhR-mediated CYP1A4...
  47. ncbi request reprint Key amino acids in the aryl hydrocarbon receptor predict dioxin sensitivity in avian species
    Jessica A Head
    Department of Biology, Centre for Advanced Research in Environmental Genomics, University of Ottawa, Ottawa, Ontario, Canada
    Environ Sci Technol 42:7535-41. 2008
    ..Given the large degree of variation in species sensitivity and the paucity of species-specific toxicity data, a genetic screen based on these findings could substantially improve risk assessment for dioxin-like compounds in wild birds...
  48. ncbi request reprint Naturally produced halogenated dimethyl bipyrroles bind to the aryl hydrocarbon receptor and induce cytochrome P4501A and porphyrin accumulation in chicken embryo hepatocytes
    Sheryl A Tittlemier
    Centre for Analytical and Environmental Chemistry, Carleton University, Ottawa, Ontario K1S 5B6, Canada
    Environ Toxicol Chem 22:1622-31. 2003
    ..000017. The results of this work illustrate that HDBPs act as agonist ligands for the AhR in the same manner as many anthropogenic organohalogens...
  49. doi request reprint Rapid in vitro metabolism of the flame retardant triphenyl phosphate and effects on cytotoxicity and mRNA expression in chicken embryonic hepatocytes
    Guanyong Su
    Environment Canada, National Wildlife Research Centre, Carleton University, Ottawa, Ontario K1A 0H3, Canada
    Environ Sci Technol 48:13511-9. 2014
    ..Overall, this is the first reported evidence that across 27 toxicologically relevant genes, DPHP altered more transcripts than its precursor, and that TPHP is also metabolized via a hydroxylation pathway in CEH. ..
  50. doi request reprint Differences in activation of aryl hydrocarbon receptors of white sturgeon relative to lake sturgeon are predicted by identities of key amino acids in the ligand binding domain
    Jon A Doering
    Environment Canada, National Wildlife Research Centre, Ottawa, Ontario K1A 0H3, Canada
    Environ Sci Technol 49:4681-9. 2015
    ..This indicates that identities of key amino acids in the LBD of AhR2 could be predictive of both in vitro activation by DLCs and in vivo sensitivity to DLCs in these, and potentially other, fishes. ..
  51. doi request reprint Comparing the effects of tetrabromobisphenol-A, bisphenol A, and their potential replacement alternatives, TBBPA-bis(2,3-dibromopropyl ether) and bisphenol S, on cell viability and messenger ribonucleic acid expression in chicken embryonic hepatocytes
    Melissa Ma
    National Wildlife Research Centre, Environment Canada, Ottawa, Ontario, Canada
    Environ Toxicol Chem 34:391-401. 2015
    ..Characterization of the potential toxicological and molecular-level effects of these compounds will ideally be useful to chemical regulators tasked with assessing the risk of new and existing chemicals...
  52. doi request reprint Sensitivity of avian species to the aryl hydrocarbon receptor ligand 6-formylindolo [3,2-b] carbazole (FICZ)
    Reza Farmahin
    Centre for Advanced Research in Environmental Genomics, Department of Biology, University of Ottawa, Ottawa, Ontario K1N 6N5, Canada Environment Canada, National Wildlife Research Centre, Ottawa, Ontario K1A 0H3, Canada
    Chem Biol Interact 221:61-9. 2014
    ..Our results suggest that FICZ and TCDD activate avian AHR1 by different modes of interaction with AHR1. ..
  53. doi request reprint Ethoxyresorufin-O-deethylase (EROD) induction by TCDD, PeCDF and PCB 126 in bobwhite quail hepatocytes
    Stephanie P Jones
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Raven Road, Ottawa, ON, K1A 0H3, Canada
    Ecotoxicology 23:802-8. 2014
    ..17 and 0.02, respectively. The rank order of potency was PeCDF > TCDD > PCB 126. The results confirm that bobwhite quail and ring-necked pheasant hepatocytes have similar sensitivity to EROD induction by TCDD, PeCDF and PCB 126...
  54. ncbi request reprint DNA strand length and EROD activity in relation to two screening measures of genotoxic exposure in Great Lakes herring gulls
    Glen A Fox
    Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottawa, ON, Canada K1A 0H3
    Ecotoxicology 14:527-44. 2005
    ..These agents appear to be persistent bioaccumulative compounds and/or their metabolites...
  55. doi request reprint Feathers as a source of RNA for genomic studies in avian species
    Stephanie P Jones
    Environment Canada, National Wildlife Research Centre, Carleton University, 1125 Colonel By Drive, Raven Road, Ottawa, ON, K1A 0H3, Canada
    Ecotoxicology 24:55-60. 2015
    ....
  56. doi request reprint Detection of PBDE effects on mRNA expression in chicken (Gallus domesticus) neuronal cells using real-time RT-PCR and a new differential display method
    Doug Crump
    Environment Canada, National Wildlife Research Centre, 1125 Colonel By Drive, Ottawa, Ontario, Canada K1S 5B6
    Toxicol In Vitro 22:1337-43. 2008
    ..Taken together, the findings from this study indicate that this in vitro cell culture method can be used to characterize the effects of PBDEs in the avian brain...
  57. ncbi request reprint Hepatic porphyria induced by the herbicide tralkoxydim in small mammals is species-specific
    Bruce D Pauli
    Canadian Wildlife Service, National Wildlife Research Centre, Carleton University, Ottawa, Ontario K1A 0H3, Canada
    Environ Toxicol Chem 24:450-6. 2005
    ..Of the species tested, only M. musculus was susceptible to the porphyrinogenic action of tralkoxydim, and no significant accumulation of protoporphyrin was observed in any of the other species exposed to the herbicide...
  58. pmc Octylphenol (OP) alters the expression of members of the amyloid protein family in the hypothalamus of the snapping turtle, Chelydra serpentina serpentina
    Vance L Trudeau
    Department of Biology and Centre for Advanced Research in Environmental Genomics, University of Ottawa, 30 Marie Curie Street, Ottawa, Ontario, K1N 6N5, Canada
    Environ Health Perspect 110:269-75. 2002
    ..Because members of the APP gene family are involved in neuronal development, we hypothesize that OP exposure may disrupt hypothalamic development in young turtles...
  59. ncbi request reprint Gonadal feminization and halogenated environmental contaminants in common terns (Sterna hirundo): evidence that ovotestes in male embryos do not persist to the prefledgling stage
    Constance A Hart
    Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA
    Ecotoxicology 12:125-40. 2003
    ....
  60. pmc The molecular basis for differential dioxin sensitivity in birds: role of the aryl hydrocarbon receptor
    Sibel I Karchner
    Biology Department, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA
    Proc Natl Acad Sci U S A 103:6252-7. 2006
    ..These studies provide a molecular understanding of species differences in sensitivity to dioxin-like compounds and suggest an approach to using the AHR as a marker of dioxin susceptibility in wildlife...