Ralph Clinckers

Summary

Affiliation: Vrije Universiteit Brussel
Country: Belgium

Publications

  1. ncbi Quantitative in vivo microdialysis study on the influence of multidrug transporters on the blood-brain barrier passage of oxcarbazepine: concomitant use of hippocampal monoamines as pharmacodynamic markers for the anticonvulsant activity
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    J Pharmacol Exp Ther 314:725-31. 2005
  2. doi Prediction of antiepileptic drug efficacy: the use of intracerebral microdialysis to monitor biophase concentrations
    Ralph Clinckers
    Vrije Universiteit Brussel VUB, Pharmaceutical Institute, Research Group Experimental Pharmacology, Department of Pharmaceutical Chemistry and Drug Analysis labo FASC, Laarbeeklaan 103, Building G, 1090 Brussels, Belgium
    Expert Opin Drug Metab Toxicol 5:1267-77. 2009
  3. doi Pharmacological and neurochemical characterization of the involvement of hippocampal adrenoreceptor subtypes in the modulation of acute limbic seizures
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    J Neurochem 115:1595-607. 2010
  4. doi Direct enhancement of hippocampal dopamine or serotonin levels as a pharmacodynamic measure of combined antidepressant-anticonvulsant action
    Ilse Smolders
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group on Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neuropharmacology 54:1017-28. 2008
  5. pmc Inactivation of the constitutively active ghrelin receptor attenuates limbic seizure activity in rodents
    Jeanelle Portelli
    Center for Neurosciences, Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neurotherapeutics 9:658-72. 2012
  6. ncbi In vivo modulatory action of extracellular glutamate on the anticonvulsant effects of hippocampal dopamine and serotonin
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    Epilepsia 46:828-36. 2005
  7. doi Intrastrain differences in seizure susceptibility, pharmacological response and basal neurochemistry of Wistar rats
    Jeanelle Portelli
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Vrije Universiteit Brussel, Brussels, Belgium
    Epilepsy Res 87:234-46. 2009
  8. ncbi Anticonvulsant action of GBR-12909 and citalopram against acute experimentally induced limbic seizures
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neuropharmacology 47:1053-61. 2004
  9. ncbi Sigma 1 receptor-mediated increase in hippocampal extracellular dopamine contributes to the mechanism of the anticonvulsant action of neuropeptide Y
    Alfred Meurs
    Department of Neurology, U Z Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium
    Eur J Neurosci 26:3079-92. 2007
  10. ncbi Involvement of the somatostatin-2 receptor in the anti-convulsant effect of angiotensin IV against pilocarpine-induced limbic seizures in rats
    Bart Stragier
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    J Neurochem 98:1100-13. 2006

Collaborators

Detail Information

Publications28

  1. ncbi Quantitative in vivo microdialysis study on the influence of multidrug transporters on the blood-brain barrier passage of oxcarbazepine: concomitant use of hippocampal monoamines as pharmacodynamic markers for the anticonvulsant activity
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    J Pharmacol Exp Ther 314:725-31. 2005
    ..Coadministration with multidrug transporter inhibitors significantly potentiates the anticonvulsant activity of oxcarbazepine and offers opportunities for treatment of pharmacoresistant epilepsy...
  2. doi Prediction of antiepileptic drug efficacy: the use of intracerebral microdialysis to monitor biophase concentrations
    Ralph Clinckers
    Vrije Universiteit Brussel VUB, Pharmaceutical Institute, Research Group Experimental Pharmacology, Department of Pharmaceutical Chemistry and Drug Analysis labo FASC, Laarbeeklaan 103, Building G, 1090 Brussels, Belgium
    Expert Opin Drug Metab Toxicol 5:1267-77. 2009
    ..In addition, we demonstrate how microdialysis data can be used in conjunction with mechanism-based pharmacokinetic/pharmacodynamic modeling for dose selection and optimization of the therapeutic regimen for novel compounds...
  3. doi Pharmacological and neurochemical characterization of the involvement of hippocampal adrenoreceptor subtypes in the modulation of acute limbic seizures
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    J Neurochem 115:1595-607. 2010
    ....
  4. doi Direct enhancement of hippocampal dopamine or serotonin levels as a pharmacodynamic measure of combined antidepressant-anticonvulsant action
    Ilse Smolders
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group on Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neuropharmacology 54:1017-28. 2008
    ..This study highlights that the hippocampus is an important site of action of combined anticonvulsant-antidepressant and monoamine enhancing effects...
  5. pmc Inactivation of the constitutively active ghrelin receptor attenuates limbic seizure activity in rodents
    Jeanelle Portelli
    Center for Neurosciences, Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neurotherapeutics 9:658-72. 2012
    ..This constitutes a novel mechanism of anticonvulsant action, whereby an endogenous agonist reduces the activity of a constitutively active receptor...
  6. ncbi In vivo modulatory action of extracellular glutamate on the anticonvulsant effects of hippocampal dopamine and serotonin
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    Epilepsia 46:828-36. 2005
    ..The present microdialysis study investigated the modulatory role of extracellular hippocampal glutamate levels in these monoaminergic protective and proconvulsant effects...
  7. doi Intrastrain differences in seizure susceptibility, pharmacological response and basal neurochemistry of Wistar rats
    Jeanelle Portelli
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Vrije Universiteit Brussel, Brussels, Belgium
    Epilepsy Res 87:234-46. 2009
    ..This study clearly illustrates that intrastrain differences do exist from one vendor/breeding location to another, or even between rats from the same breeding location...
  8. ncbi Anticonvulsant action of GBR-12909 and citalopram against acute experimentally induced limbic seizures
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neuropharmacology 47:1053-61. 2004
    ..Non-selective actions curtailing glutamatergic activity may further be responsible for the unexpected anticonvulsant effects at predicted proconvulsant concentrations...
  9. ncbi Sigma 1 receptor-mediated increase in hippocampal extracellular dopamine contributes to the mechanism of the anticonvulsant action of neuropeptide Y
    Alfred Meurs
    Department of Neurology, U Z Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium
    Eur J Neurosci 26:3079-92. 2007
    ..This novel mechanism of anticonvulsant action of NPY is separate from, and may be complementary to, the well established Y2 receptor-mediated inhibition of hippocampal excitability...
  10. ncbi Involvement of the somatostatin-2 receptor in the anti-convulsant effect of angiotensin IV against pilocarpine-induced limbic seizures in rats
    Bart Stragier
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    J Neurochem 98:1100-13. 2006
    ....
  11. doi Hippocampal sst(1) receptors are autoreceptors and do not affect seizures in rats
    Dimitri De Bundel
    Department of Pharmaceutical Chemistry, Research Group Experimental Neuropharmacology, Drug Analysis and Drug Information, Vrije Universiteit Brussel, Brussels, Belgium
    Neuroreport 21:254-8. 2010
    ....
  12. doi Rat hippocampal somatostatin sst3 and sst4 receptors mediate anticonvulsive effects in vivo: indications of functional interactions with sst2 receptors
    Najat Aourz
    Center for Neuroscience, Department of Pharmaceutical Chemistry and Drug Analysis, CePhar, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neuropharmacology 61:1327-33. 2011
    ..Nevertheless, selective sst(2) receptor antagonism prevented these sst(3)- or sst(4) receptor-mediated anticonvulsant effects, suggesting a functional cooperation with rat hippocampal sst(2) receptors...
  13. ncbi Hippocampal dopamine and serotonin elevations as pharmacodynamic markers for the anticonvulsant efficacy of oxcarbazepine and 10,11-dihydro-10-hydroxycarbamazepine
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neurosci Lett 390:48-53. 2005
    ..These pharmacodynamic markers are here shown to be useful for the selection of anticonvulsant threshold concentrations of oxcarbazepine and 10,11-dihydro-10-hydroxycarbamazepine...
  14. ncbi Substantia nigra is an anticonvulsant site of action of topiramate in the focal pilocarpine model of limbic seizures
    Alfred Meurs
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    Epilepsia 47:1519-35. 2006
    ..Prompted by the observation that intrahippocampal topiramate (TPM) administration does not suppress limbic seizures in the focal pilocarpine model, we investigated the role of the SNR in the anticonvulsant mechanism of action of TPM...
  15. ncbi Anticonvulsant action of hippocampal dopamine and serotonin is independently mediated by D and 5-HT receptors
    Ralph Clinckers
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Brussels, Belgium
    J Neurochem 89:834-43. 2004
    ..Our results suggest that, within a certain concentration range, DA and 5-HT contribute independently to the prevention of hippocampal epileptogenesis via, respectively, D(2) and 5-HT(1A) receptor activation...
  16. doi Neuropeptide Y increases in vivo hippocampal extracellular glutamate levels through Y1 receptor activation
    Alfred Meurs
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Center for Neuroscience, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Neurosci Lett 510:143-7. 2012
    ..Whilst this finding contrasts with the supposed glutamatergic hypothesis for NPY in the hippocampus, it is of significance to further assist in deciphering NPY's mechanisms of action in in vivo settings...
  17. doi The antidepressants citalopram and reboxetine reduce seizure frequency in rats with chronic epilepsy
    Katia Vermoesen
    Center for Neurosciences, Department of Pharmaceutical Chemistry and Drug Analysis, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels, Belgium
    Epilepsia 53:870-8. 2012
    ..The present study was designed to determine the seizure liability of citalopram and reboxetine in the kainic acid-induced post-status epilepticus model for temporal lobe epilepsy...
  18. doi Assessment of the convulsant liability of antidepressants using zebrafish and mouse seizure models
    Katia Vermoesen
    Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neuroscience, Vrije Universiteit Brussel, Brussels, Belgium
    Epilepsy Behav 22:450-60. 2011
    ..In general we conclude that the convulsant liability of antidepressants is model and concentration dependent...
  19. doi The control of kainic acid-induced status epilepticus
    Katia Vermoesen
    Department of Pharmaceutical Chemistry and Drug Analysis, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Epilepsy Res 90:164-6. 2010
    ..Diazepam and ketamine synergistically blocked all electrographical seizure activity...
  20. doi Involvement of the AT1 receptor subtype in the effects of angiotensin IV and LVV-haemorphin 7 on hippocampal neurotransmitter levels and spatial working memory
    Dimitri De Bundel
    Research Group Experimental Pharmacology, Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Vrije Universiteit Brussel, Brussels, Belgium
    J Neurochem 112:1223-34. 2010
    ..c.v. injection, suggesting an extrahippocampal site of action. We propose that AT1 receptors are implicated in the neurochemical and cognitive effects of Ang IV, whereas LVV-H7 may mediate its effects via IRAP...
  21. doi Determination of reboxetine in rat brain microdialysates and plasma samples using liquid chromatography coupled to fluorescence detection
    Naser Shraim
    Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neurosciences, Laarbeeklaan 103, B 1090 Brussels, Belgium
    J Chromatogr B Analyt Technol Biomed Life Sci 898:53-61. 2012
    ..7% and 11.5% with an average recovery of 98.5±7.3% (mean±SD, n=40). We demonstrated the applicability of the method to determine the concentration-time profiles of RBX in brain and plasma following systemic administration...
  22. ncbi Clinical potential of neuropeptide Y receptor ligands in the treatment of epilepsy
    Alfred Meurs
    Department of Neurology, University Hospital Brussels, Laarbeeklaan 101, 1090 Brussels, Belgium
    Curr Top Med Chem 7:1660-74. 2007
    ....
  23. ncbi Use of microbore LC-MS/MS for the quantification of oxcarbazepine and its active metabolite in rat brain microdialysis samples
    Katrien Lanckmans
    Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Research Group Experimental Pharmacology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    J Chromatogr B Analyt Technol Biomed Life Sci 831:205-12. 2006
    ..By applying this method, we were able to determine the extracellular concentrations of OXC and MHD during at least 4h after intraperitoneal (i.p.) administration of 10 mg/kg OXC...
  24. ncbi In vivo modulation of extracellular hippocampal glutamate and GABA levels and limbic seizures by group I and II metabotropic glutamate receptor ligands
    Ilse Smolders
    Department of Pharmaceutical Chemistry, Research Group Experimental Pharmacology, Pharmaceutical Institute, Vrije Universiteit Brussel VUB, Brussels, Belgium
    J Neurochem 88:1068-77. 2004
    ..These data provide a neurochemical basis for the full anticonvulsant effects of mGlu1a and mGlu5 antagonists and the partial effects observed with mGlu2/3 agonists in vivo...
  25. pmc White-matter astrocytes, axonal energy metabolism, and axonal degeneration in multiple sclerosis
    Melissa Cambron
    Department of Neurology, Center for Neurosciences, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Brussel, Belgium
    J Cereb Blood Flow Metab 32:413-24. 2012
    ..White-matter astrocytes may be considered as a potential target for neuroprotective MS therapies...
  26. doi Microbore liquid chromatography with UV detection to study the in vivo passage of compound 21, a non-peptidergic AT₂ receptor agonist, to the striatum in rats
    Naser Shraim
    Vrije Universiteit Brussel, Department of Pharmaceutical Chemistry and Drug Analysis, Center for Neuroscience, Laarbeeklaan 103, B 1090 Brussels, Belgium
    J Neurosci Methods 202:137-42. 2011
    ....
  27. doi Astrocytes as potential targets to suppress inflammatory demyelinating lesions in multiple sclerosis
    Jacques de Keyser
    Department of Neurology, University Hospital Brussel, Vrije Universiteit Brussel, Brussels, Belgium
    Neurochem Int 57:446-50. 2010
    ..If confirmed and extended by additional studies, suppressing the antigen presenting capacity of astrocytes could be a novel therapeutic option for the treatment of MS...
  28. doi Astrocytic beta(2)-adrenergic receptors: from physiology to pathology
    Guy Laureys
    Department of Pharmaceutical Chemistry and Drug Analysis, Vrije Universiteit Brussel, Belgium
    Prog Neurobiol 91:189-99. 2010
    ..In this review we focus on the physiological functions of astrocytic beta(2)-adrenergic receptors, and their possible impact in disease states...