Johan Robbens

Summary

Affiliation: University of Antwerp
Country: Belgium

Publications

  1. ncbi request reprint Ecotoxicological risk assessment using DNA chips and cellular reporters
    Johan Robbens
    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171 B 2020, Antwerp, Belgium
    Trends Biotechnol 25:460-6. 2007
  2. doi request reprint Performance of the flow cytometric E-screen assay in screening estrogenicity of pure compounds and environmental samples
    Caroline Vanparys
    Laboratory of Ecophysiology, Biochemistry and Toxicology, University of Antwerp, Antwerp, Belgium
    Sci Total Environ 408:4451-60. 2010
  3. doi request reprint Selection and characterization of PCB-binding DNA aptamers
    Jaytry Mehta
    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Anal Chem 84:1669-76. 2012
  4. doi request reprint cDNA phage display as a novel tool to screen for cellular targets of chemical compounds
    Bieke Van Dorst
    Department of Biology, Laboratory for Ecophysiology, University Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Toxicol In Vitro 24:1435-40. 2010
  5. doi request reprint In vitro selection and characterization of DNA aptamers recognizing chloramphenicol
    Jaytry Mehta
    University of Antwerp, Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan, Antwerp, Belgium
    J Biotechnol 155:361-9. 2011
  6. doi request reprint Selection of scFv phages specific for chloramphenicol acetyl transferase (CAT), as alternatives for antibodies in CAT detection assays
    Bieke Van Dorst
    University Antwerp, Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan 171, B 2020, Antwerp, Belgium
    J Appl Toxicol 32:783-9. 2012
  7. doi request reprint Phage display as a method for discovering cellular targets of small molecules
    Bieke Van Dorst
    University Antwerp, Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Methods 58:56-61. 2012
  8. doi request reprint Aptasensing of chloramphenicol in the presence of its analogues: reaching the maximum residue limit
    Sanaz Pilehvar
    Department of Chemistry, University of Antwerp, Universiteitsplein 1, B 2610 Antwerp, Belgium
    Anal Chem 84:6753-8. 2012
  9. doi request reprint Recent advances in recognition elements of food and environmental biosensors: a review
    Bieke Van Dorst
    University of Antwerp, Department of Biology, Laboratory of Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan 171, 2020 Antwerp, Belgium
    Biosens Bioelectron 26:1178-94. 2010
  10. doi request reprint Phage display as a novel screening tool for primary toxicological targets
    Bieke Van Dorst
    Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Environ Toxicol Chem 29:250-5. 2010

Detail Information

Publications23

  1. ncbi request reprint Ecotoxicological risk assessment using DNA chips and cellular reporters
    Johan Robbens
    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171 B 2020, Antwerp, Belgium
    Trends Biotechnol 25:460-6. 2007
    ..Some important technical and interpretative hurdles still need to be overcome before a full implementation of ecotoxicogenomics in regulatory settings will occur...
  2. doi request reprint Performance of the flow cytometric E-screen assay in screening estrogenicity of pure compounds and environmental samples
    Caroline Vanparys
    Laboratory of Ecophysiology, Biochemistry and Toxicology, University of Antwerp, Antwerp, Belgium
    Sci Total Environ 408:4451-60. 2010
    ..Therefore, this study, among other E-screen studies, supports the use of MCF-7 cell proliferation as estrogenicity screen for pure compounds and complex samples...
  3. doi request reprint Selection and characterization of PCB-binding DNA aptamers
    Jaytry Mehta
    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Anal Chem 84:1669-76. 2012
    ..This study therefore advances aptamer technology by targeting one of the highly sought-after POPs, for the first time ever recorded...
  4. doi request reprint cDNA phage display as a novel tool to screen for cellular targets of chemical compounds
    Bieke Van Dorst
    Department of Biology, Laboratory for Ecophysiology, University Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Toxicol In Vitro 24:1435-40. 2010
    ....
  5. doi request reprint In vitro selection and characterization of DNA aptamers recognizing chloramphenicol
    Jaytry Mehta
    University of Antwerp, Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan, Antwerp, Belgium
    J Biotechnol 155:361-9. 2011
    ..8 and 1μM respectively). These aptamers open up possibilities to allow easy detection of Cam via aptamer-based biosensors...
  6. doi request reprint Selection of scFv phages specific for chloramphenicol acetyl transferase (CAT), as alternatives for antibodies in CAT detection assays
    Bieke Van Dorst
    University Antwerp, Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan 171, B 2020, Antwerp, Belgium
    J Appl Toxicol 32:783-9. 2012
    ..1 ng ml⁻¹ or 4 pM. This low detection limit demonstrates the potential of the scFv phages as an alternative for capturing antibodies in a highly sensitive detection test to measure CAT concentrations in reporter gene assays...
  7. doi request reprint Phage display as a method for discovering cellular targets of small molecules
    Bieke Van Dorst
    University Antwerp, Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Methods 58:56-61. 2012
    ....
  8. doi request reprint Aptasensing of chloramphenicol in the presence of its analogues: reaching the maximum residue limit
    Sanaz Pilehvar
    Department of Chemistry, University of Antwerp, Universiteitsplein 1, B 2610 Antwerp, Belgium
    Anal Chem 84:6753-8. 2012
    ..Its simplicity and low detection limit (because of the home-selected aptamers) suggest that the electrochemical aptasensor is suitable for practical use in the detection of CAP in milk samples...
  9. doi request reprint Recent advances in recognition elements of food and environmental biosensors: a review
    Bieke Van Dorst
    University of Antwerp, Department of Biology, Laboratory of Ecophysiology, Biochemistry and Toxicology, Groenenborgerlaan 171, 2020 Antwerp, Belgium
    Biosens Bioelectron 26:1178-94. 2010
    ....
  10. doi request reprint Phage display as a novel screening tool for primary toxicological targets
    Bieke Van Dorst
    Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Environ Toxicol Chem 29:250-5. 2010
    ..This shows that phage display, which is already widely used in other research fields, also has potential in ecotoxicology, as a novel screening tool with which to determine primary toxicological targets of chemical compounds...
  11. pmc Toxicity ranking and toxic mode of action evaluation of commonly used agricultural adjuvants on the basis of bacterial gene expression profiles
    Ingrid Nobels
    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Antwerp, Belgium
    PLoS ONE 6:e24139. 2011
    ..Although the number of compounds that were evaluated is rather limited (13), the results show that the used reporter assay is a promising tool to rank commonly used agricultural adjuvants based on toxicity and toxic mode of action data...
  12. doi request reprint Expression characteristics of potential biomarker genes in Tra catfish, Pangasianodon hypophthalmus, exposed to trichlorfon
    Amit Kumar Sinha
    Laboratory for Ecophysiology, Biochemistry and Toxicology, University of Antwerp, 2020 Antwerp, Belgium
    Comp Biochem Physiol Part D Genomics Proteomics 5:207-16. 2010
    ..These results could permit to develop a "molecular biomarker system" which can be applied as a first-tier method of identifying contaminant exposure before effects at population level occur...
  13. ncbi request reprint Effect of copper exposure on gene expression profiles in Chlamydomonas reinhardtii based on microarray analysis
    An Jamers
    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerp, Belgium
    Aquat Toxicol 80:249-60. 2006
    ....
  14. ncbi request reprint Cadmium and zinc induction of ZnT-1 mRNA in an established carp cell line
    Frederik Muylle
    Department of Biology, Laboratory for Ecophysiology, Biochemistry and Toxicology, University of Antwerp, Belgium
    Comp Biochem Physiol C Toxicol Pharmacol 143:242-51. 2006
    ..Our results clearly show that although Zn and Cd can both regulate Zn export in EPC cells, the effects on the cellular Zn pools are quite different...
  15. doi request reprint Carbon nanotubes based electrochemical aptasensing platform for the detection of hydroxylated polychlorinated biphenyl in human blood serum
    Sanaz Pilehvar
    University of Antwerp, Department of Chemistry, Groenenborgerlaan 171, 2020 Antwerp, Belgium
    Biosens Bioelectron 54:78-84. 2014
    ..The described approach for aptasensing opens up new perspectives in the field of biomonitoring providing a device with acceptable stability, high sensitivity, good accuracy and precision...
  16. pmc Aptamer-based molecular recognition of lysergamine, metergoline and small ergot alkaloids
    Elsa Rouah-Martin
    Laboratory of Systemic Physiological and Ecotoxicological Research SPHERE, Department of Biology, University of Antwerp, Groenenborgerlaan 171, Antwerp B 2020, Belgium
    Int J Mol Sci 13:17138-59. 2012
    ..This system could also be applied to small ergot alkaloids in an ergot contaminated flour sample...
  17. doi request reprint The identification of cellular targets of 17β estradiol using a lytic (T7) cDNA phage display approach
    Bieke Van Dorst
    University Antwerp, Department of Biology, Antwerp, Belgium
    Toxicol In Vitro 25:388-93. 2011
    ..This illustrates the potential of lytic (T7) cDNA phage display in toxicology, to provide important information about cellular targets of chemical compounds...
  18. ncbi request reprint Ecological impact assessment of metallurgic effluents using in situ biomarker assays
    Wim De Coen
    Laboratory for Ecophysiology, Biochemistry and Toxicology, Department of Biology, University of Antwerp, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Environ Pollut 141:283-94. 2006
    ..In addition, we found clear genotoxic effects caused by the industrial discharges that might correlate with a reduction in (long-term) survival...
  19. doi request reprint Folding properties of the hepatitis B core as a carrier protein for vaccination research
    Michiel Etienne Janssens
    Department of Animal Health, Institute for Tropical Medicine, 155 Nationalestraat, 2000, Antwerp, Belgium
    Amino Acids 38:1617-26. 2010
    ..In this study, we also propose a new high-yield purification protocol for fusion proteins to be used in vaccination experiments with the carrier protein or in comparative studies of particulate or non-particulate HBc fusion proteins...
  20. ncbi request reprint Expression, purification and characterization of full-length RNA-free hepatitis B core particles
    Katleen Broos
    Laboratory of Plant Physiology, Department of Biology, 171 Groenenborgerlaan, Building U, 6th Floor, CGB University of Antwerp, 2020 Antwerpen, Belgium
    Protein Expr Purif 54:30-7. 2007
    ..In this report, we detail a novel method for the synthesis and purification of full-length core particles essentially free of RNA from Escherichia coli...
  21. pmc Estrogen-like properties of fluorotelomer alcohols as revealed by mcf-7 breast cancer cell proliferation
    Marleen Maras
    Laboratory for Ecophysiology, Biochemistry, and Toxicology, University of Antwerp, Antwerp, Belgium
    Environ Health Perspect 114:100-5. 2006
    ..The latter finding suggests an alternative mode of action of the fluorotelomer alcohols compared with that of E2. This study clearly underlines the need for future in vivo testing for specific endocrine-related end points...
  22. doi request reprint Conventional and (eco) toxicological assessment of batch partial ozone oxidation and subsequent biological treatment of a tank truck cleaning generated concentrate
    Wim De Schepper
    University of Antwerp UA, Department of Biology, Ecophysiology, Biochemistry and Toxicology Group EBT, Groenenborgerlaan 171, B 2020 Antwerp, Belgium
    Water Res 43:4037-49. 2009
    ..subcapitata algal 72h growth inhibition test. The 500 mg O(3)/l optimal ozone dosage, as determined from P. subcapitata algal 72h growth inhibition and S(COD) removal, could be retrieved using short-term methods...
  23. ncbi request reprint Response of DNA fragments to potentiometric sensors studied using HPLC
    Luc J Nagels
    Antwerp University, Chemistry Department, Groenenborgerlaan 171, B 2020, Antwerp, Belgium
    Comb Chem High Throughput Screen 10:555-9. 2007
    ..The slopes of the calibration graphs were at least 23 mV/decade (dT(10)), which was much higher than expected. The results are discussed in view of the potential use of this sensor type in high throughput microarrays...