Ghislain Opdenakker

Summary

Affiliation: Katholieke Universiteit Leuven
Country: Belgium

Publications

  1. ncbi request reprint Remnant epitopes, autoimmunity and glycosylation
    Ghislain Opdenakker
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Belgium
    Biochim Biophys Acta 1760:610-5. 2006
  2. pmc Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy
    Ahmed M Abu El-Asrar
    Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia
    Lipids Health Dis 13:187. 2014
  3. ncbi request reprint Targeting matrix metalloproteinases in acute inflammatory shock syndromes
    Zheng Qiu
    School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
    Comb Chem High Throughput Screen 15:555-70. 2012
  4. doi request reprint Probing cytokines, chemokines and matrix metalloproteinases towards better immunotherapies of multiple sclerosis
    Ghislain Opdenakker
    Laboratories of Immunobiology and Molecular Immunology, Rega Institute for Medical Research, K U Leuven, B 3000 Leuven, Belgium
    Cytokine Growth Factor Rev 22:359-65. 2011
  5. ncbi request reprint A monoclonal antibody inhibits gelatinase B/MMP-9 by selective binding to part of the catalytic domain and not to the fibronectin or zinc binding domains
    Erik Martens
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Biochim Biophys Acta 1770:178-86. 2007
  6. pmc Rescue from acute neuroinflammation by pharmacological chemokine-mediated deviation of leukocytes
    Nele Berghmans
    Rega Institute for Medical Research, Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium
    J Neuroinflammation 9:243. 2012
  7. ncbi request reprint Synergistic induction of CXCL9 and CXCL11 by Toll-like receptor ligands and interferon-gamma in fibroblasts correlates with elevated levels of CXCR3 ligands in septic arthritis synovial fluids
    Paul Proost
    Laboratories of Molecular Immunology, Rega Institute for Medical Research, Leuven, Belgium
    J Leukoc Biol 75:777-84. 2004
  8. pmc Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies
    Paul Proost
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium
    Arthritis Res Ther 8:R107. 2006
  9. ncbi request reprint TLR ligands and cytokines induce CXCR3 ligands in endothelial cells: enhanced CXCL9 in autoimmune arthritis
    Tamara Loos
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    Lab Invest 86:902-16. 2006
  10. pmc Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation
    Paul Proost
    Laboratory of Molecular Immunology, Rega Institute, K U Leuven, Leuven, Belgium
    J Exp Med 205:2085-97. 2008

Detail Information

Publications86

  1. ncbi request reprint Remnant epitopes, autoimmunity and glycosylation
    Ghislain Opdenakker
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Belgium
    Biochim Biophys Acta 1760:610-5. 2006
    ....
  2. pmc Expression of bioactive lysophospholipids and processing enzymes in the vitreous from patients with proliferative diabetic retinopathy
    Ahmed M Abu El-Asrar
    Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia
    Lipids Health Dis 13:187. 2014
    ..In addition, we investigated the correlations between the levels of PA and LPA and the levels of the inflammatory and endothelial dysfunction biomarker soluble vascular cell adhesion molecule-1 (sVCAM-1)...
  3. ncbi request reprint Targeting matrix metalloproteinases in acute inflammatory shock syndromes
    Zheng Qiu
    School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China
    Comb Chem High Throughput Screen 15:555-70. 2012
    ..This evaluation strengthens the expectation that MMP inhibitors, in particular those blocking neutrophil proteases, may become useful in the early phase of acute inflammatory shock syndromes...
  4. doi request reprint Probing cytokines, chemokines and matrix metalloproteinases towards better immunotherapies of multiple sclerosis
    Ghislain Opdenakker
    Laboratories of Immunobiology and Molecular Immunology, Rega Institute for Medical Research, K U Leuven, B 3000 Leuven, Belgium
    Cytokine Growth Factor Rev 22:359-65. 2011
    ..Novel molecules interfering with matrix metalloproteinases and chemokines, such as EMMPRIN, COAM and monoclonal antibodies are currently being investigated, demonstrating continued efforts to find new drugs for MS treatment...
  5. ncbi request reprint A monoclonal antibody inhibits gelatinase B/MMP-9 by selective binding to part of the catalytic domain and not to the fibronectin or zinc binding domains
    Erik Martens
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Biochim Biophys Acta 1770:178-86. 2007
    ..These results define part of the catalytic domain of gelatinase B/MMP-9, and not the zinc-binding or fibronectin domains, as target for the development of selective inhibitors...
  6. pmc Rescue from acute neuroinflammation by pharmacological chemokine-mediated deviation of leukocytes
    Nele Berghmans
    Rega Institute for Medical Research, Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium
    J Neuroinflammation 9:243. 2012
    ..However, in view of unsatisfactory results and severe side effects, complementary therapies are needed. We have examined the effect of chlorite-oxidized oxyamylose (COAM), a potent antiviral polycarboxylic acid on EAE...
  7. ncbi request reprint Synergistic induction of CXCL9 and CXCL11 by Toll-like receptor ligands and interferon-gamma in fibroblasts correlates with elevated levels of CXCR3 ligands in septic arthritis synovial fluids
    Paul Proost
    Laboratories of Molecular Immunology, Rega Institute for Medical Research, Leuven, Belgium
    J Leukoc Biol 75:777-84. 2004
    ..These results illustrate the complex interaction between cytokines and TLR ligands in infection...
  8. pmc Coexpression and interaction of CXCL10 and CD26 in mesenchymal cells by synergising inflammatory cytokines: CXCL8 and CXCL10 are discriminative markers for autoimmune arthropathies
    Paul Proost
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium
    Arthritis Res Ther 8:R107. 2006
    ..Synovial concentrations of the neutrophil chemoattractant CXCL8 may therefore be useful to discriminate between autoimmune arthritis types...
  9. ncbi request reprint TLR ligands and cytokines induce CXCR3 ligands in endothelial cells: enhanced CXCL9 in autoimmune arthritis
    Tamara Loos
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    Lab Invest 86:902-16. 2006
    ..Thus, CXCL9 is an important chemokine in autoimmune arthritis...
  10. pmc Citrullination of CXCL8 by peptidylarginine deiminase alters receptor usage, prevents proteolysis, and dampens tissue inflammation
    Paul Proost
    Laboratory of Molecular Immunology, Rega Institute, K U Leuven, Leuven, Belgium
    J Exp Med 205:2085-97. 2008
    ..This study shows that PAD citrullinates the chemokine CXCL8, and thus may dampen neutrophil extravasation during acute or chronic inflammation...
  11. doi request reprint CXCR3 determines strain susceptibility to murine cerebral malaria by mediating T lymphocyte migration toward IFN-gamma-induced chemokines
    Philippe E Van Den Steen
    Laboratory of Immunobiology, Rega Institute for Medical Research, Catholic University of Leuven, Leuven, Belgium
    Eur J Immunol 38:1082-95. 2008
    ..The strain-dependent susceptibility to develop CM is more related to the expression of CXCR3 in circulating leukocytes than to the chemokine expression levels in the brain...
  12. ncbi request reprint Stimulation of angiostatic platelet factor-4 variant (CXCL4L1/PF-4var) versus inhibition of angiogenic granulocyte chemotactic protein-2 (CXCL6/GCP-2) in normal and tumoral mesenchymal cells
    Jo Vandercappellen
    Laboratory of Molecular Immunology, Rega Institute, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    J Leukoc Biol 82:1519-30. 2007
    ..Application of angiostatic CXCL4L1/PF-4var without attraction of protumoral phagocytes may be beneficial in cancer therapy...
  13. doi request reprint "Reverse degradomics", monitoring of proteolytic trimming by multi-CE and confocal detection of fluorescent substrates and reaction products
    Helene Piccard
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    Electrophoresis 30:2366-77. 2009
    ....
  14. ncbi request reprint GCP-2/CXCL6 synergizes with other endothelial cell-derived chemokines in neutrophil mobilization and is associated with angiogenesis in gastrointestinal tumors
    Klara Gijsbers
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    Exp Cell Res 303:331-42. 2005
    ....
  15. doi request reprint Immunopathology and dexamethasone therapy in a new model for malaria-associated acute respiratory distress syndrome
    Philippe E Van Den Steen
    Laboratory of Immunobiology, Rega Institute for Medical Research, Catholic University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Am J Respir Crit Care Med 181:957-68. 2010
    ..No efficient treatments are available for MA-ARDS and its pathogenesis remains poorly understood...
  16. ncbi request reprint Carboxyterminal cleavage of the chemokines MIG and IP-10 by gelatinase B and neutrophil collagenase
    Philippe E Van Den Steen
    Laboratory of Molecular Immunology, Rega Institute, University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Biochem Biophys Res Commun 310:889-96. 2003
    ..Alignment of the CXC chemokines with the respective cleavage sites by both MMPs identified the ELR motif as a possible determinant for amino terminal cleavage by these MMPs...
  17. doi request reprint Expression of angiostatic platelet factor-4var/CXCL4L1 counterbalances angiogenic impulses of vascular endothelial growth factor, interleukin-8/CXCL8, and stromal cell-derived factor 1/CXCL12 in esophageal and colorectal cancer
    Hannelien Verbeke
    Rega Institute for Medical Research, Laboratory of Molecular Immunology, K U Leuven, 3000 Leuven, Belgium
    Hum Pathol 41:990-1001. 2010
    ....
  18. doi request reprint Beta-hematin interaction with the hemopexin domain of gelatinase B/MMP-9 provokes autocatalytic processing of the propeptide, thereby priming activation by MMP-3
    Nathalie Geurts
    Laboratory of Immunobiology, Rega Institute for Medical Research, Catholic University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Biochemistry 47:2689-99. 2008
    ..These findings suggest that reduction of hemorrhage and hemolysis might prevent matrix metalloproteinase-9-mediated inflammatory and vascular damages...
  19. ncbi request reprint Gelatinase B/matrix metalloproteinase-9 provokes cataract by cleaving lens betaB1 crystallin
    Francis J Descamps
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    FASEB J 19:29-35. 2005
    ..The cleavage of betaB1 crystallin was also observed in vivo after endogenous gelatinase B-induction by the chemokine granulocyte chemotactic protein-2 in wild-type mice but not in gelatinase B-/- mice...
  20. ncbi request reprint Microbial Toll-like receptor ligands differentially regulate CXCL10/IP-10 expression in fibroblasts and mononuclear leukocytes in synergy with IFN-gamma and provide a mechanism for enhanced synovial chemokine levels in septic arthritis
    Paul Proost
    Laboratory of Molecular Immunology, Rega Institute, K U Leuven, Leuven, Belgium
    Eur J Immunol 33:3146-53. 2003
    ....
  21. pmc PARC/CCL18 is a plasma CC chemokine with increased levels in childhood acute lymphoblastic leukemia
    Sofie Struyf
    Laboratories of Molecular Immunology, Rega Institute, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Am J Pathol 163:2065-75. 2003
    ..Serum PARC levels thus constitute a novel leukemia marker, possibly reflecting tumor/host cell interactions in the circulation...
  22. doi request reprint Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis
    Armando M De Palma
    Laboratory of Virology and Experimental Chemotherapy, Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Virology 382:20-7. 2008
    ..In summary, this report demonstrates that acute coxsackievirus B4-induced pancreatitis involves the action of MMP-9, which is mainly originating from macrophages and neutrophils...
  23. ncbi request reprint DNA polymerase mutations in drug-resistant herpes simplex virus mutants determine in vivo neurovirulence and drug-enzyme interactions
    Graciela Andrei
    Laboratory of Virology, Rega Institute for Medical Research, K U Leuven, Belgium
    Antivir Ther 12:719-32. 2007
    ..It appears that drug-resistant mutants arising under the pressure of HPMP derivatives have the lowest levels of neurovirulence...
  24. doi request reprint Myeloid cells are tunable by a polyanionic polysaccharide derivative and co-determine host rescue from lethal virus infection
    Sandra Li
    Rega Institute for Medical Research, Department of Microbiology and Immunology, University of Leuven, Minderbroedersstraat 10, Leuven, Belgium
    J Leukoc Biol 88:1017-29. 2010
    ..These findings reinforce the role of neutrophils and macrophages as critical cells that can be manipulated toward antiviral defense...
  25. doi request reprint Hemozoin induces lung inflammation and correlates with malaria-associated acute respiratory distress syndrome
    Katrien Deroost
    Laboratory of Immunobiology, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven University of Leuven, Leuven 3000, Belgium
    Am J Respir Cell Mol Biol 48:589-600. 2013
    ..Thus, hemozoin correlates with MA-ARDS and induces pulmonary inflammation...
  26. doi request reprint CC chemokine ligand-2 synergizes with the nonchemokine G protein-coupled receptor ligand fMLP in monocyte chemotaxis, and it cooperates with the TLR ligand LPS via induction of CXCL8
    Mieke Gouwy
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, B 3000 Leuven, Belgium
    J Leukoc Biol 86:671-80. 2009
    ....
  27. ncbi request reprint Remnant epitopes generate autoimmunity: from rheumatoid arthritis and multiple sclerosis to diabetes
    Francis J Descamps
    Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Adv Exp Med Biol 535:69-77. 2003
    ..In addition, gelatinase B is upregulated in pancreatitis and cleaves insulin. The most efficient cleavage by gelatinase B leads to a major insulin remnant epitope...
  28. doi request reprint Synergistic up-regulation of MCP-2/CCL8 activity is counteracted by chemokine cleavage, limiting its inflammatory and anti-tumoral effects
    Sofie Struyf
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, K U Leuven, Leuven, Belgium
    Eur J Immunol 39:843-57. 2009
    ..These data indicate that optimal induction and delivery of MCP-2/CCL8 is counteracted by converting this chemokine into a receptor antagonist, thereby losing its anti-tumoral potential...
  29. doi request reprint Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
    Helene Piccard
    Rega Institute for Medical Research, University of Leuven, Belgium
    Int J Cancer 131:E425-36. 2012
    ..Thus, COAM constitutes a novel tool to study the role of innate immune cells in the initial stages of tumor development and an example that innate immunostimulating glycosaminoglycan mimicry may be exploited therapeutically...
  30. ncbi request reprint Gelatinase B/matrix metalloproteinase-9 cleaves interferon-beta and is a target for immunotherapy
    Inge Nelissen
    Rega Institute for Medical Research, Laboratory of Molecular Immunology, University of Leuven, Leuven, Belgium
    Brain 126:1371-81. 2003
    ....
  31. doi request reprint Isotypic neutralizing antibodies against mouse GCP-2/CXCL6 inhibit melanoma growth and metastasis
    Hannelien Verbeke
    Rega Institute for Medical Research, Laboratory of Molecular Immunology, K U Leuven, Leuven, Belgium
    Cancer Lett 302:54-62. 2011
    ..Moreover, the drop in muGCP-2 antibody titer correlated with the melanoma tumor size. Taken together, we show that functional blocking of GCP-2 inhibits tumor growth and metastases...
  32. doi request reprint Interleukin-17 regulates chemokine and gelatinase B expression in fibroblasts to recruit both neutrophils and monocytes
    Zheng Qiu
    Rega Institute for Medical Research, University of Leuven, Belgium
    Immunobiology 214:835-42. 2009
    ..We conclude that IL-17 regulates differentially chemokine and MMP expression by normal and transformed fibroblasts and is indirectly capable of attracting both monocytes and neutrophils to the inflammatory focus...
  33. ncbi request reprint Hemopexin domains as multifunctional liganding modules in matrix metalloproteinases and other proteins
    Helene Piccard
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    J Leukoc Biol 81:870-92. 2007
    ....
  34. ncbi request reprint Inhibition of lethal endotoxin shock with an L-pyridylalanine containing metalloproteinase inhibitor selected by high-throughput screening of a new peptide library
    Jialiang Hu
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Comb Chem High Throughput Screen 9:599-611. 2006
    ..These data illustrate the usefulness of the screening platform for protective inhibitor discovery and complement recent insights in the pathogenesis and treatment of shock syndromes...
  35. pmc Citrullination of CXCL8 increases this chemokine's ability to mobilize neutrophils into the blood circulation
    Tamara Loos
    Laboratory of Molecular Immunology, Rega Institute, K U Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Haematologica 94:1346-53. 2009
    ..Recently, we discovered a new natural post-translational modification of CXCL8, i.e. the deimination of arginine into citrulline by peptidylarginine deiminases...
  36. ncbi request reprint Gelatinase B/MMP-9 and neutrophil collagenase/MMP-8 process the chemokines human GCP-2/CXCL6, ENA-78/CXCL5 and mouse GCP-2/LIX and modulate their physiological activities
    Philippe E Van Den Steen
    Laboratory of Molecular Immunology, Rega Institute, University of Leuven, Belgium
    Eur J Biochem 270:3739-49. 2003
    ..This rapid conversion was also performed by crude neutrophil granule secretion under physiological conditions, extending the relevance of this proteolytic cleavage to the in vivo situation...
  37. ncbi request reprint In vivo activation of gelatinase B/MMP-9 by trypsin in acute pancreatitis is a permissive factor in streptozotocin-induced diabetes
    Francis J Descamps
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Minderbroederstraat 10, B 3000 Leuven, Belgium
    J Pathol 204:555-61. 2004
    ..In conclusion, gelatinase B itself is not a causative factor but, when activated by endogenous trypsin, is a permissive factor for insulin degradation and diabetes...
  38. ncbi request reprint Chemokines synergize in the recruitment of circulating neutrophils into inflamed tissue
    Sofie Struyf
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, Leuven, Belgium
    Eur J Immunol 35:1583-91. 2005
    ..These data demonstrate that inducible and constitutive GPCR ligands synergize to enhance inflammation and facilitate a more effective immune response...
  39. pmc Improved methods for haemozoin quantification in tissues yield organ-and parasite-specific information in malaria-infected mice
    Katrien Deroost
    Laboratory of Immunobiology, Rega Institute, University of Leuven, Leuven, Belgium
    Malar J 11:166. 2012
    ..This study aimed to improve methods for Hz quantification in tissues and to investigate the accumulation of Hz in different organs from mice infected with Plasmodium parasites with a varying degree of virulence...
  40. doi request reprint Adenylyl cyclase-associated protein-1/CAP1 as a biological target substrate of gelatinase B/MMP-9
    Bénédicte Cauwe
    Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Exp Cell Res 314:2739-49. 2008
    ..The high turnover rate of CAP1 by MMP-9, comparable to that of gelatin as the natural extracellular substrate of this enzyme, may be critical to prevent pathological conditions associated with considerable cytolysis...
  41. pmc Angiostatic and chemotactic activities of the CXC chemokine CXCL4L1 (platelet factor-4 variant) are mediated by CXCR3
    Sofie Struyf
    Rega Institute, K U Leuven, Leuven, Belgium
    Blood 117:480-8. 2011
    ..However, the in vivo data indicate that the angiostatic property of CXCL4L1 is equally important in retarding tumor growth. Thus, both CXCR3A and CXCR3B are implicated in the chemotactic and vascular effects of CXCL4L1...
  42. doi request reprint Deficiency of gelatinase B/MMP-9 aggravates lpr-induced lymphoproliferation and lupus-like systemic autoimmune disease
    Bénédicte Cauwe
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    J Autoimmun 36:239-52. 2011
    ....
  43. ncbi request reprint The hemopexin and O-glycosylated domains tune gelatinase B/MMP-9 bioavailability via inhibition and binding to cargo receptors
    Philippe E Van Den Steen
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, B 3000, Belgium
    J Biol Chem 281:18626-37. 2006
    ..Therefore, the OG and hemopexin domains down-regulate the bioavailability of active MMP-9 and the interactions with the cargo receptors are proposed to be the original function of hemopexin domains in MMPs...
  44. ncbi request reprint Matrix metalloproteinase inhibitors as therapy for inflammatory and vascular diseases
    Jialiang Hu
    Rega Institute for Medical Research, Catholic University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Nat Rev Drug Discov 6:480-98. 2007
    ....
  45. doi request reprint Serum amyloid A1α induces paracrine IL-8/CXCL8 via TLR2 and directly synergizes with this chemokine via CXCR2 and formyl peptide receptor 2 to recruit neutrophils
    Mieke De Buck
    Laboratory of Molecular Immunology and Laboratory of Immunobiology, Rega Institute for Medical Research, Department of Microbiology and Immunology, University of Leuven, Leuven, Belgium
    J Leukoc Biol 98:1049-60. 2015
    ..g., lipopolysaccharide) inflammatory mediators induce primary chemoattractants such as serum amyloid A that synergize in an autocrine (monocyte) or a paracrine (neutrophil) fashion with secondary chemokines induced in stromal cells. ..
  46. doi request reprint Pathogenesis of malaria-associated acute respiratory distress syndrome
    Philippe E Van Den Steen
    Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Trends Parasitol 29:346-58. 2013
    ..Experimental anti-inflammatory interventions are successful in mice and remain to be validated in patients. ..
  47. doi request reprint Zymography methods for visualizing hydrolytic enzymes
    Jennifer Vandooren
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, KU Leuven, Belgium
    Nat Methods 10:211-20. 2013
    ..Future development of novel substrate probes and improvement in detection and imaging methods will increase the applicability of zymography for (reverse) degradomics studies...
  48. doi request reprint Meprins process matrix metalloproteinase-9 (MMP-9)/gelatinase B and enhance the activation kinetics by MMP-3
    Nathalie Geurts
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    FEBS Lett 586:4264-9. 2012
    ..Hence, this meprin-mediated processing and enhancement of MMP-9 activation kinetics may have biological relevance in the context of in vivo inflammatory processes...
  49. pmc Insufficiently defined genetic background confounds phenotypes in transgenic studies as exemplified by malaria infection in Tlr9 knockout mice
    Nathalie Geurts
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    PLoS ONE 6:e27131. 2011
    ....
  50. doi request reprint Inflammatory rather than infectious insults play a role in exocrine tissue damage in a mouse model for coxsackievirus B4-induced pancreatitis
    Armando M De Palma
    Laboratory of Virology and Experimental Chemotherapy, Rega Institute for Medical Research, University of Leuven, Belgium
    J Pathol 217:633-41. 2009
    ..In conclusion, our data suggest that immunopathological effects, rather than direct virus-induced destruction, are responsible for the damage to acinar tissue in CVB4-induced pancreatitis...
  51. ncbi request reprint Matrix metalloproteinases, tissue inhibitors of MMPs and TACE in experimental cerebral malaria
    Philippe E Van Den Steen
    Laboratory of Immunobiology, Rega Institute, University of Leuven, Leuven, Belgium
    Lab Invest 86:873-88. 2006
    ..berghei ANKA. Furthermore, in comparison with C57Bl/6 mice, a larger increase in TIMP-1 and a marked, >30-fold induction in MMP-3 were found in the brains of Balb/C mice, suggesting possible protective roles for TIMP-1 and MMP-3...
  52. doi request reprint Interferon-γ orchestrates the number and function of Th17 cells in experimental autoimmune encephalomyelitis
    Nele Berghmans
    Laboratory of Immunobiology, Faculty of Medicine, Rega Institute, University of Leuven, Leuven, Belgium
    J Interferon Cytokine Res 31:575-87. 2011
    ..IFN-γ thus orchestrates the number and function of Th17 cells...
  53. ncbi request reprint Recombinant mouse granulocyte chemotactic protein-2: production in bacteria, characterization, and systemic effects on leukocytes
    Sofie Starckx
    Rega Institute for Medical Research, Laboratory for Molecular Immunology, University of Leuven, Leuven, Belgium
    J Interferon Cytokine Res 22:965-74. 2002
    ..These data reinforce the idea that chemokines, including GCP-2, influence the homeostasis of circulating leukocyte numbers...
  54. ncbi request reprint A novel rationale for inhibition of gelatinase B in multiple sclerosis: MMP-9 destroys alpha B-crystallin and generates a promiscuous T cell epitope
    Sofie Starckx
    Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium
    J Neuroimmunol 141:47-57. 2003
    ..Gelatinase B is thus able to release T cell epitopes from intact alphaB-crystallin, but their pathogenic role remains unclear...
  55. ncbi request reprint The activated form of gelatinase B/matrix metalloproteinase-9 is associated with diabetic vitreous hemorrhage
    Francis J Descamps
    Laboratory of Immunobiology, Rega Institute, University of Leuven, Belgium
    Exp Eye Res 83:401-7. 2006
    ..001) and strongly correlated with hemoglobin levels (r=0.7525; p<0.001). Activated MMP-9 was not detected in paired serum samples. We conclude that activated MMP-9 might be involved in hemorrhagic transformation in patients with PDR...
  56. ncbi request reprint Targeting neutrophil collagenase/matrix metalloproteinase-8 and gelatinase B/matrix metalloproteinase-9 with a peptidomimetic inhibitor protects against endotoxin shock
    Jialiang Hu
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, Leuven 3000, Belgium
    Biochem Pharmacol 70:535-44. 2005
    ..This proves the principle that early neutrophil MMP inhibition followed by TACE blockade may become a treatment strategy of gram-negative sepsis, endotoxinemia and other life-threatening inflammatory reactions...
  57. pmc Angiostatic, tumor inflammatory and anti-tumor effects of CXCL4(47-70) and CXCL4L1(47-70) in an EGF-dependent breast cancer model
    Katrien Van Raemdonck
    Laboratory of Molecular Immunology, KU Leuven University of Leuven, Department of Microbiology and Immunology, Rega Institute for Medical Research, Leuven, Belgium
    Oncotarget 5:10916-33. 2014
    ..Our results suggest a tumor type-dependent responsiveness to either CXCL4(47-70) or CXCL4L1(47-70) treatment, defined by anti-proliferative, angiostatic and inflammatory actions, and substantiate their therapeutic potential...
  58. doi request reprint Heterogeneity and evolution of thymidine kinase and DNA polymerase mutants of herpes simplex virus type 1: implications for antiviral therapy
    Graciela Andrei
    Laboratory of Virology, Rega Institute for Medical Research, K U Leuven, Minderbroedersstraat 10, Leuven, Belgium
    J Infect Dis 207:1295-305. 2013
    ..An understanding of the evolutionary aspects of HSV infection is crucial to the design of effective therapeutic and control strategies...
  59. ncbi request reprint Gelatinase B is diabetogenic in acute and chronic pancreatitis by cleaving insulin
    Francis J Descamps
    Rega Institute for Medical Research, Laboratory of Molecular Immunology, University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    FASEB J 17:887-9. 2003
    ..Consequently, diabetes may be worsened by enzymatic degradation of insulin by gelatinase B and by the consequent enhancement of the autoimmune process. Gelatinase B is diabetogenic in acute and chronic pancreatitis by cleaving insulin...
  60. ncbi request reprint The CXC chemokine GCP-2/CXCL6 is predominantly induced in mesenchymal cells by interleukin-1beta and is down-regulated by interferon-gamma: comparison with interleukin-8/CXCL8
    Anja Wuyts
    Laboratory of Molecular Immunology, Rega Institute, University of Leuven, Leuven, Belgium
    Lab Invest 83:23-34. 2003
    ..The patterns of producer cell types, inducers and kinetics and the quantities of GCP-2 produced, suggest a unique role for GCP-2 in physiologic and pathologic processes...
  61. ncbi request reprint The molecular biology of matrix metalloproteinases and tissue inhibitors of metalloproteinases in inflammatory bowel diseases
    Magali de Bruyn
    a Laboratory of Immunobiology, Department of Microbiology and Immunology, KU Leuven, Rega Institute for Medical Research, Leuven, Belgium
    Crit Rev Biochem Mol Biol 51:295-358. 2016
    ....
  62. pmc Hemozoin induces hepatic inflammation in mice and is differentially associated with liver pathology depending on the Plasmodium strain
    Katrien Deroost
    Department of Microbiology and Immunology, Laboratory of Immunobiology, Rega Institute for Medical Research, KU Leuven University of Leuven, Leuven, Belgium
    PLoS ONE 9:e113519. 2014
    ..falciparum-derived hemozoin in malaria-free C57BL/6J mice induced inflammatory gene transcription in the liver, suggesting that hemozoin may be involved in the pathogenesis of malaria hepatopathy by inducing inflammation. ..
  63. pmc Intradermal air pouch leukocytosis as an in vivo test for nanoparticles
    Jennifer Vandooren
    Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    Int J Nanomedicine 8:4745-56. 2013
    ....
  64. doi request reprint Biochemistry and molecular biology of gelatinase B or matrix metalloproteinase-9 (MMP-9): the next decade
    Jennifer Vandooren
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, KU Leuven, Belgium
    Crit Rev Biochem Mol Biol 48:222-72. 2013
    ..Such studies will better define conditions in which inhibition of MMP-9 is beneficial or has to be avoided...
  65. doi request reprint Virus entry inhibition by chlorite-oxidized oxyamylose versus induction of antiviral interferon by poly(I:C)
    Sandra Li
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium
    Biochem Pharmacol 76:831-40. 2008
    ..These results suggest that COAM can alter the outcome of infection, not by IFN induction and in turn modifying the cellular antiviral state, but through inhibition of virus entry into cells...
  66. pmc Organotypic epithelial raft cultures as a model for evaluating compounds against alphaherpesviruses
    Graciela Andrei
    Rega Institute for Medical Research, Catholic University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Antimicrob Agents Chemother 49:4671-80. 2005
    ..Since no animal model is available for the evaluation of antiviral agents against VZV, the organotypic cultures may be considered a model to evaluate the efficacy of new anti-VZV antivirals before clinical trials...
  67. doi request reprint The thymidine phosphorylase inhibitor 5'-O-tritylinosine (KIN59) is an antiangiogenic multitarget fibroblast growth factor-2 antagonist
    Sandra Liekens
    Laboratory of Virology and Chemotherapy, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium
    Mol Cancer Ther 11:817-29. 2012
    ....
  68. ncbi request reprint Neutralizing antibodies in gene-defective hosts
    Ghislain Opdenakker
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, Belgium
    Trends Immunol 24:94-100. 2003
    ..This Review compares and exemplifies the various genetic and immunological contexts that lead to 'neutralizing and generated by gene defect' or 'nagged' antibodies, and outlines implications and solutions for therapeutic strategies...
  69. ncbi request reprint Biochemistry and molecular biology of gelatinase B or matrix metalloproteinase-9 (MMP-9)
    Philippe E Van Den Steen
    Rega Institute for Medical Research, University of Leuven, Belgium
    Crit Rev Biochem Mol Biol 37:375-536. 2002
    ..These include reproduction, growth, development, inflammation, and vascular and proliferative diseases...
  70. ncbi request reprint Simulation of evolution-selected propeptide by high-throughput selection of a peptidomimetic inhibitor on a capillary DNA sequencer platform
    Jialiang Hu
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Leuven 3000, Belgium
    Anal Chem 77:2116-24. 2005
    ..A notable finding is that the in vitro-selected inhibitor mimics many of the characteristics of the evolution-selected MMP propeptide sequence...
  71. ncbi request reprint The countercurrent principle in invasion and metastasis of cancer cells. Recent insights on the roles of chemokines
    Ghislain Opdenakker
    Rega Institute for Medical Research, University Leuven, B 3000 Leuven, Belgium
    Int J Dev Biol 48:519-27. 2004
    ....
  72. ncbi request reprint Role of matrix metalloproteinase-9 in a mouse model for amyotrophic lateral sclerosis
    Maarten Dewil
    Laboratory for Neurobiology, Campus Gasthuisberg, Herestraat 49, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Neuroreport 16:321-4. 2005
    ..Therefore, the effect of pharmacological inhibition of MMP-9 activity is unlikely to be of therapeutical benefit in amyotrophic lateral sclerosis...
  73. ncbi request reprint Chemokine-protease interactions in cancer
    Jo Van Damme
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    Semin Cancer Biol 14:201-8. 2004
    ..Although proteolytical processing of chemokines is well studied in vitro, the direct or indirect effects on tumour invasion and metastasis are only poorly evaluated...
  74. ncbi request reprint The biochemical, biological, and pathological kaleidoscope of cell surface substrates processed by matrix metalloproteinases
    Bénédicte Cauwe
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Leuven, Belgium
    Crit Rev Biochem Mol Biol 42:113-85. 2007
    ....
  75. doi request reprint Multidimensional degradomics identifies systemic autoantigens and intracellular matrix proteins as novel gelatinase B/MMP-9 substrates
    Bénédicte Cauwe
    Department of Microbiology and Immunology, Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, Leuven, Belgium
    Integr Biol (Camb) 1:404-26. 2009
    ..The study of proteolysis of ICM molecules, autoantigens, alarmins and other crucial intracellular molecules may result in the discovery of novel roles for proteolytic modification...
  76. doi request reprint Intracellular substrate cleavage: a novel dimension in the biochemistry, biology and pathology of matrix metalloproteinases
    Bénédicte Cauwe
    Rega Institute for Medical Research, Laboratory of Immunobiology, University of Leuven, Minderbroedersstraat 10, 3000 Leuven, Belgium
    Crit Rev Biochem Mol Biol 45:351-423. 2010
    ..Hence, further elucidation of intracellular MMP localizations and intracellular substrate proteolysis is a new challenge in MMP research...
  77. ncbi request reprint Functional roles and therapeutic targeting of gelatinase B and chemokines in multiple sclerosis
    Ghislain Opdenakker
    Rega Institute for Medical Research, University of Leuven, Belgium
    Lancet Neurol 2:747-56. 2003
    ..This may allow interferon beta to be used at lower doses and prevent side-effects...
  78. doi request reprint Enzymatic degradation of adhesive-dentin interfaces produced by mild self-etch adhesives
    Jan De Munck
    Department of Conservative Dentistry, Leuven BIOMAT Research Cluster, School of Dentistry, Oral Pathology and Maxillo Facial Surgery, Catholic University of Leuven, Leuven, Belgium
    Eur J Oral Sci 118:494-501. 2010
    ..It is concluded that in contrast to etch-and-rinse adhesives, the involvement of endogenous MMP-2 and MMP-9 in the bond-degradation process is minimal for mild self-etch adhesives...
  79. doi request reprint Matrix metalloproteinases as therapeutic targets in protozoan parasitic infections
    Nathalie Geurts
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Leuven, Minderbroedersstraat 10, B3000 Leuven, Belgium
    Pharmacol Ther 133:257-79. 2012
    ..MMP inhibitors (MMPIs) may dampen collateral tissue damage, as is anecdotically reported for tetracyclines as MMP regulators in parasite infections...
  80. ncbi request reprint Inhibitors of gelatinase B/matrix metalloproteinase-9 activity comparison of a peptidomimetic and polyhistidine with single-chain derivatives of a neutralizing monoclonal antibody
    Jialiang Hu
    Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10, Leuven, Belgium
    Biochem Pharmacol 67:1001-9. 2004
    ..As an extra control and serendipitously it was found that polyhistidine is an inhibitor of gelatinases, presumably by altering the active site by chelation of the catalytic Zn2+...
  81. pmc Gelatin degradation assay reveals MMP-9 inhibitors and function of O-glycosylated domain
    Jennifer Vandooren
    Jennifer Vandooren, Nathalie Geurts, Erik Martens, Philippe E Van den Steen, Ghislain Opdenakker, Laboratory of Immunobiology, Rega Institute for Medical Research, University of Leuven, Minderbroederstraat 10, Leuven B 3000, Belgium
    World J Biol Chem 2:14-24. 2011
    ..To establish a novel, sensitive and high-throughput gelatinolytic assay to define new inhibitors and compare domain deletion mutants of gelatinase B/matrix metalloproteinase (MMP)-9...
  82. ncbi request reprint Identification of biologically active chemokine isoforms from ascitic fluid and elevated levels of CCL18/pulmonary and activation-regulated chemokine in ovarian carcinoma
    Evemie Schutyser
    Laboratory of Molecular Immunology, Rega Institute for Medical Research, K U Leuven, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    J Biol Chem 277:24584-93. 2002
    ..This points to a delicate balance of chemokines in epithelial ovarian cancer and to a potentially major role for CXCL8 and CCL18 in this tumor...
  83. ncbi request reprint Cleavage of denatured natural collagen type II by neutrophil gelatinase B reveals enzyme specificity, post-translational modifications in the substrate, and the formation of remnant epitopes in rheumatoid arthritis
    Philippe E Van Den Steen
    Rega Institute for Medical Research, Laboratory of Molecular Immunology, University of Leuven, 3000 Leuven, Belgium
    FASEB J 16:379-89. 2002
    ..These data lend support to the thesis that extracellular proteolysis and other post-translational modifications of antigenic peptides may be critical in the establishment and perpetuation of autoimmune processes...
  84. ncbi request reprint Gelatinase B deficiency protects against endotoxin shock
    Benedicte Dubois
    Rega Institute, Laboratory of Molecular Immunology, University of Leuven, Leuven, Belgium
    Eur J Immunol 32:2163-71. 2002
    ..The gelatinase B-deficient mice were resistant to endotoxin shock, which implies that specific MMP-9 inhibition constitutes anapproach for the treatment of septic shock syndromes...
  85. ncbi request reprint Regulation of gelatinase B in human monocytic and endothelial cells by PECAM-1 ligation and its modulation by interferon-beta
    Inge Nelissen
    Rega Institute for Medical Research, Laboratory of Molecular Immunology, University of Leuven, B 3000 Leuven, Belgium
    J Leukoc Biol 71:89-98. 2002
    ..IFN-beta did not alter PECAM-1 expression on PBMCs. Thus, PECAM-1 and gelatinase B are differently regulated in leukocytes and endothelial cells...
  86. pmc Characterization of herpes simplex virus type 1 thymidine kinase mutants selected under a single round of high-dose brivudin
    Graciela Andrei
    Katholieke Universiteit Leuven, Rega Institute for Medical Research, Laboratory of Virology, Minderbroedersstraat 10, B 3000 Leuven, Belgium
    J Virol 79:5863-9. 2005
    ..The A168T change, associated with an altered TK phenotype, proved to be the most commonly selected substitution. For the different mutants, a correlation between phenotype, genotype, and in vivo neurovirulence was observed...