Denis Dufrane

Summary

Country: Belgium

Publications

  1. ncbi request reprint The influence of implantation site on the biocompatibility and survival of alginate encapsulated pig islets in rats
    Denis Dufrane
    Faculte de Medecine, Laboratory of Experimental Surgery, Universite Catholique de Louvain, CHEX 5570, Avenue Hippocrate 55, B 1200 Brussels, Belgium
    Biomaterials 27:3201-8. 2006
  2. ncbi request reprint Parameters favouring successful adult pig islet isolations for xenotransplantation in pig-to-primate models
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculty of Medicine, Brussels, Belgium
    Xenotransplantation 13:204-14. 2006
  3. doi request reprint Regeneration of abdominal wall musculofascial defects by a human acellular collagen matrix
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, CHEX 5570, Avenue Hippocrate 55, B 1200 Brussels, Belgium
    Biomaterials 29:2237-48. 2008
  4. doi request reprint Native pancreatic alpha-cell adaptation in streptozotocin-induced diabetic primates: importance for pig islet xenotransplantation
    Denis Dufrane
    Faculte de Medecine, Laboratory of Experimental Surgery, Universite Catholique de Louvain, CHEX 5570, Avenue Hippocrate 55, Brussels, Belgium
    Xenotransplantation 16:152-63. 2009
  5. doi request reprint Alginate macroencapsulation of pig islets allows correction of streptozotocin-induced diabetes in primates up to 6 months without immunosuppression
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Transplantation 90:1054-62. 2010
  6. ncbi request reprint Six-month survival of microencapsulated pig islets and alginate biocompatibility in primates: proof of concept
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, and Department of Pathology, Cliniques Universitaires Saint Luc, Brussels, Belgium
    Transplantation 81:1345-53. 2006
  7. doi request reprint Improvement of pig islet function by in vivo pancreatic tissue remodeling: a "human-like" pig islet structure with streptozotocin treatment
    Sophie Veriter
    Pôle de Chirurgie Expérimentale et Transplantation, Laboratory of Experimental Surgery, Universite Catholique de Louvain, Secteur des Sciences de la Santé, Brussels, Belgium
    Cell Transplant 22:2161-73. 2013
  8. doi request reprint In vivo selection of biocompatible alginates for islet encapsulation and subcutaneous transplantation
    Sophie Veriter
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Tissue Eng Part A 16:1503-13. 2010
  9. doi request reprint Galactosyl-knock-out engineered pig as a xenogenic donor source of adipose MSCs for bone regeneration
    Thomas Schubert
    Endocrine Cell Therapy Unit, Center of Tissue and Cell Therapy, Cliniques Universitaires Saint Luc, Universite Catholique de Louvain, 1200 Brussels, Belgium
    Biomaterials 34:3279-89. 2013
  10. doi request reprint Improvement of subcutaneous bioartificial pancreas vascularization and function by coencapsulation of pig islets and mesenchymal stem cells in primates
    Sophie Veriter
    Universite Catholique de Louvain, Secteur des Sciences de la Santé, Institute of Clinical and Experimental Research, Pôle de Chirurgie Expérimentale et Transplantation, Brussels, Belgium
    Cell Transplant 23:1349-64. 2014

Collaborators

Detail Information

Publications27

  1. ncbi request reprint The influence of implantation site on the biocompatibility and survival of alginate encapsulated pig islets in rats
    Denis Dufrane
    Faculte de Medecine, Laboratory of Experimental Surgery, Universite Catholique de Louvain, CHEX 5570, Avenue Hippocrate 55, B 1200 Brussels, Belgium
    Biomaterials 27:3201-8. 2006
    ..In rats, the peritoneum site seems not appropriate for promoting the engraftment of encapsulated pig islets. Kidney subcapsular and subcutaneous spaces represent an interesting alternative...
  2. ncbi request reprint Parameters favouring successful adult pig islet isolations for xenotransplantation in pig-to-primate models
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculty of Medicine, Brussels, Belgium
    Xenotransplantation 13:204-14. 2006
    ..This experimental work was designed to provide guidelines applicable in pig pancreas procurement and islet isolation for successful islet xenotransplantation into primates...
  3. doi request reprint Regeneration of abdominal wall musculofascial defects by a human acellular collagen matrix
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, CHEX 5570, Avenue Hippocrate 55, B 1200 Brussels, Belgium
    Biomaterials 29:2237-48. 2008
    ..In conclusion, human acellular collagen matrix, placed in an FLT position, can induce an abdominal wall reconstitution without adhesions and hernia recurrence...
  4. doi request reprint Native pancreatic alpha-cell adaptation in streptozotocin-induced diabetic primates: importance for pig islet xenotransplantation
    Denis Dufrane
    Faculte de Medecine, Laboratory of Experimental Surgery, Universite Catholique de Louvain, CHEX 5570, Avenue Hippocrate 55, Brussels, Belgium
    Xenotransplantation 16:152-63. 2009
    ....
  5. doi request reprint Alginate macroencapsulation of pig islets allows correction of streptozotocin-induced diabetes in primates up to 6 months without immunosuppression
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Transplantation 90:1054-62. 2010
    ..This study assessed the capacity of alginate-encapsulated islets to reverse diabetes in a pig-to-primate model...
  6. ncbi request reprint Six-month survival of microencapsulated pig islets and alginate biocompatibility in primates: proof of concept
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, and Department of Pathology, Cliniques Universitaires Saint Luc, Brussels, Belgium
    Transplantation 81:1345-53. 2006
    ..The aim of this study was to assess the long-term biocompatibility of alginate encapsulated pig islets after transplantation in primates...
  7. doi request reprint Improvement of pig islet function by in vivo pancreatic tissue remodeling: a "human-like" pig islet structure with streptozotocin treatment
    Sophie Veriter
    Pôle de Chirurgie Expérimentale et Transplantation, Laboratory of Experimental Surgery, Universite Catholique de Louvain, Secteur des Sciences de la Santé, Brussels, Belgium
    Cell Transplant 22:2161-73. 2013
    ..05) and insulin release (144 µU/ml) than nontreated islets (59 µU/ml, p < 0.05), respectively. Low-dose STZ (<50 mg/kg) can modify the structure of pig islets in vivo and improve insulin secretion after isolation. ..
  8. doi request reprint In vivo selection of biocompatible alginates for islet encapsulation and subcutaneous transplantation
    Sophie Veriter
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Tissue Eng Part A 16:1503-13. 2010
    ..A traditional alginate made of high mannuronic content (SLM3%) is an adapted material to immunoprotect islets in subcutaneous tissue. No improvement was found with lower viscosity and use of GRGDSP-peptide sequence...
  9. doi request reprint Galactosyl-knock-out engineered pig as a xenogenic donor source of adipose MSCs for bone regeneration
    Thomas Schubert
    Endocrine Cell Therapy Unit, Center of Tissue and Cell Therapy, Cliniques Universitaires Saint Luc, Universite Catholique de Louvain, 1200 Brussels, Belgium
    Biomaterials 34:3279-89. 2013
    ..No significant difference was found at POD 28. The combination of the cellular immunomodulation with the Gal-KO phenotype of AMSCs can significantly improve the cellular engraftment of pig osteogenic cells by delaying xenorejection...
  10. doi request reprint Improvement of subcutaneous bioartificial pancreas vascularization and function by coencapsulation of pig islets and mesenchymal stem cells in primates
    Sophie Veriter
    Universite Catholique de Louvain, Secteur des Sciences de la Santé, Institute of Clinical and Experimental Research, Pôle de Chirurgie Expérimentale et Transplantation, Brussels, Belgium
    Cell Transplant 23:1349-64. 2014
    ..However, the cotransplantation of islets with MSCs improves only slightly the long-term function of a subcutaneous bioartificial pancreas in a primate preclinical model. ..
  11. pmc Macro- or microencapsulation of pig islets to cure type 1 diabetes
    Denis Dufrane
    Department of Health Sciences, Institute for Experimental and Clinical, Experimental Surgery and Transplantation, Catholic University of Louvain, B 1200 Brussels, Belgium
    World J Gastroenterol 18:6885-93. 2012
    ..In this review, we summarize current knowledge of encapsulated pig islets, to determine obstacles to implantation in humans and possible solutions to overcome these obstacles...
  12. doi request reprint Beta-5 score to evaluate pig islet graft function in a primate pre-clinical model
    Yasuhiro Igarashi
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Xenotransplantation 17:449-59. 2010
    ..We developed a composite scoring system to accurately assess pig islet function in pre-clinical primate studies...
  13. doi request reprint Critical size bone defect reconstruction by an autologous 3D osteogenic-like tissue derived from differentiated adipose MSCs
    Thomas Schubert
    Endocrine Cell Therapy Unit, Center of Tissue and Cell Therapy, Cliniques Universitaires Saint Luc, Universite Catholique de Louvain, 1200 Brussels, Belgium
    Biomaterials 34:4428-38. 2013
    ..This custom-made 3D osteogenic AMSC autograft is a therapeutic solution for bone non-unions and for critical-size defects...
  14. doi request reprint Pig islets for clinical islet xenotransplantation
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Curr Opin Nephrol Hypertens 18:495-500. 2009
    ..Another source of insulin-producing cells, therefore, would be of major interest, and pigs represent a possible and serious source for obtaining such cells...
  15. doi request reprint Pig islet for xenotransplantation in human: structural and physiological compatibility for human clinical application
    Denis Dufrane
    Pôle de Chirurgie Expérimentale et Transplantation, Universite Catholique de Louvain, Secteur des Sciences de la Santé, Institut de Recherche Expérimentale et Clinique, B 1200 Brussels, Belgium
    Transplant Rev (Orlando) 26:183-8. 2012
    ..Therefore, in this review, we provide a summary of the present state of knowledge about pig and human islet compatibility...
  16. ncbi request reprint Streptozotocin-induced diabetes in large animals (pigs/primates): role of GLUT2 transporter and beta-cell plasticity
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Transplantation 81:36-45. 2006
    ..To induce irreversible diabetes in large animals, the efficiency of streptozotocin (STZ) was evaluated in pigs, primates and compared to the gold standard model in rats...
  17. pmc Human Adipose-Derived Mesenchymal Stem Cells in Cell Therapy: Safety and Feasibility in Different "Hospital Exemption" Clinical Applications
    Sophie Veriter
    Endocrine Cell Therapy, Centre of Tissular and Cellular Therapy, Cliniques Universitaires Saint Luc, Brussels, Belgium
    PLoS ONE 10:e0139566. 2015
    ..We demonstrated that autologous ASC transplantation can be considered a safe and feasible therapy tool for extreme clinical indications of ASC properties and physiopathology of disease. ..
  18. pmc Scaffold-free Three-dimensional Graft From Autologous Adipose-derived Stem Cells for Large Bone Defect Reconstruction: Clinical Proof of Concept
    Denis Dufrane
    From the Endocrine Cell Therapy, Center of Tissue and Cell Therapy, Cliniques Universitaires Saint Luc, Brussels, Belgium DD, WA, NA Orthopedic Surgery Service, Department of Surgery, Cliniques Universitaires Saint Luc, Brussels, Belgium P LD, CD and Center for Human Genetics, Cliniques Universitaires Saint Luc Université Catholique de Louvain, Brussels, Belgium HAP
    Medicine (Baltimore) 94:e2220. 2015
    ..We demonstrated that this engineered tissue can safely promote osteogenesis in extreme conditions of bone nonunions with minor donor site morbidity and no oncological side effects...
  19. ncbi request reprint Nutrient control of insulin secretion in isolated normal human islets
    Jean Claude Henquin
    Unité d Endocrinologie et Métabolisme, UCL 55 30, Ave Hippocrate 55, B 1200 Brussels, Belgium
    Diabetes 55:3470-7. 2006
    ....
  20. pmc Application of Electron Paramagnetic Resonance (EPR) Oximetry to Monitor Oxygen in Wounds in Diabetic Models
    Céline M Desmet
    Biomedical Magnetic Resonance Research Group, Louvain Drug Research Institute, Universite Catholique de Louvain, Brussels, Belgium
    PLoS ONE 10:e0144914. 2015
    ..Nevertheless, the technique is limited for measurements in pedicled skin flaps and cannot be applied to excisional wounds in which diffusion of atmospheric oxygen significantly affects the measurements. ..
  21. doi request reprint The enhanced performance of bone allografts using osteogenic-differentiated adipose-derived mesenchymal stem cells
    Thomas Schubert
    Laboratory of Experimental Surgery IREC CHEX, Universite Catholique de Louvain, Faculte de Medecine, Brussels, Belgium
    Biomaterials 32:8880-91. 2011
    ..05)) were found for osteogenic-differentiated AMSCs in comparison to BM-MSCs after 30 days of implantation. Osteogenic-differentiated AMSCs are the best candidate to improve the angio-/osteogenicity of decellularized bone allografts...
  22. doi request reprint A pharmacological approach to understanding the control of insulin secretion in human islets
    Jean Claude Henquin
    Unit of Endocrinology and Metabolism, Faculty of Medicine, University of Louvain, Brussels, Belgium
    Diabetes Obes Metab . 2017
    ..To understand better the control of insulin secretion (IS) by human β-cells and identify similarities and differences with rodent models...
  23. ncbi request reprint A simple method using a polymethylpenten chamber for isolation of human pancreatic islets
    Denis Dufrane
    Laboratory of Experimental Surgery, Universite Catholique de Louvain, Faculty of Medicine, Brussels, Belgium
    Pancreas 30:e51-9. 2005
    ..We describe a novel method using a polymethylpenten chamber (PMPC) and compare its efficacy to the classic method using a stainless steel chamber (SSC)...
  24. doi request reprint Formalin fixation could interfere with the clinical assessment of the tumor-free margin in tumor surgery: magnetic resonance imaging-based study
    Pierre Louis Docquier
    Department of Orthopedic Surgery, Saint Luc University Hospital, Brussels, Belgium
    Oncology 78:115-24. 2010
    ..Fixation could interfere with the accuracy of the assessment of the tumor-free margin...
  25. pmc Impact of Hyperglycemia and Low Oxygen Tension on Adipose-Derived Stem Cells Compared with Dermal Fibroblasts and Keratinocytes: Importance for Wound Healing in Type 2 Diabetes
    Aurore Lafosse
    Universite Catholique de Louvain, Brussels, Belgium
    PLoS ONE 11:e0168058. 2016
    ....
  26. doi request reprint Dynamics of glucose-induced insulin secretion in normal human islets
    Jean Claude Henquin
    Unit of Endocrinology and Metabolism, Faculty of Medicine, University of Louvain, Brussels, Belgium
    Am J Physiol Endocrinol Metab 309:E640-50. 2015
    ....
  27. doi request reprint Autologous Adipose Stromal Cells Seeded onto a Human Collagen Matrix for Dermal Regeneration in Chronic Wounds: Clinical Proof of Concept
    Aurore Lafosse
    Brussels, Belgium From the Endocrine Cell Therapy Unit, Center of Tissue Cell Therapy, Institut de Recherche Expérimentale et Clinique, Plastic and Reconstructive Surgery Unit, Louvain Drug Research Institute, Biomedical Magnetic Resonance Research Unit, Center for Human Genetics and Human Molecular Genetics, de Duve Institute, Cliniques Universitaires Saint Luc, Cardiovascular Research Unit, Institut de Recherche Expérimentale et Clinique, Universite Catholique de Louvain
    Plast Reconstr Surg 136:279-95. 2015
    ..This work aimed to develop a biological dressing made of adipose-derived stromal cells onto a human acellular collagen matrix...