Bernd Mayer

Summary

Affiliation: University of Graz
Country: Austria

Publications

  1. pmc Tolerance to nitroglycerin through proteasomal down-regulation of aldehyde dehydrogenase-2 in a genetic mouse model of ascorbate deficiency
    G Wölkart
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Br J Pharmacol 168:1868-77. 2013
  2. pmc Cardiac dysfunction in adipose triglyceride lipase deficiency: treatment with a PPARα agonist
    G Wölkart
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Br J Pharmacol 165:380-9. 2012
  3. pmc The enigma of nitroglycerin bioactivation and nitrate tolerance: news, views and troubles
    B Mayer
    Department of Pharmacology and Toxicology, Karl Franzens University Graz, Graz, Austria
    Br J Pharmacol 155:170-84. 2008
  4. doi request reprint Inactivation of soluble guanylate cyclase by stoichiometric S-nitrosation
    Bernd Mayer
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Mol Pharmacol 75:886-91. 2009
  5. pmc Characterization of the East Asian variant of aldehyde dehydrogenase-2: bioactivation of nitroglycerin and effects of Alda-1
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, 8010 Graz, Austria
    J Biol Chem 285:943-52. 2010
  6. doi request reprint Mitochondrial nitrite reduction coupled to soluble guanylate cyclase activation: lack of evidence for a role in the bioactivation of nitroglycerin
    Alexander Kollau
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Nitric Oxide 20:53-60. 2009
  7. pmc Role of the general base Glu-268 in nitroglycerin bioactivation and superoxide formation by aldehyde dehydrogenase-2
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Biol Chem 284:19878-86. 2009
  8. doi request reprint Potent inhibition of aldehyde dehydrogenase-2 by diphenyleneiodonium: focus on nitroglycerin bioactivation
    Regina Neubauer
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Austria
    Mol Pharmacol 84:407-14. 2013
  9. pmc Effects of nitroglycerin/L-cysteine on soluble guanylate cyclase: evidence for an activation/inactivation equilibrium controlled by nitric oxide binding and haem oxidation
    Antonius C F Gorren
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Biochem J 390:625-31. 2005
  10. doi request reprint Tetrahydrobiopterin protects soluble guanylate cyclase against oxidative inactivation
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 82:420-7. 2012

Collaborators

Detail Information

Publications63

  1. pmc Tolerance to nitroglycerin through proteasomal down-regulation of aldehyde dehydrogenase-2 in a genetic mouse model of ascorbate deficiency
    G Wölkart
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Br J Pharmacol 168:1868-77. 2013
    ....
  2. pmc Cardiac dysfunction in adipose triglyceride lipase deficiency: treatment with a PPARα agonist
    G Wölkart
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Br J Pharmacol 165:380-9. 2012
    ..In the present study we investigated cardiac function in ATGL-deficient mice and the potential therapeutic effects of the PPARα and γ agonists Wy14,643 and rosiglitazone, respectively...
  3. pmc The enigma of nitroglycerin bioactivation and nitrate tolerance: news, views and troubles
    B Mayer
    Department of Pharmacology and Toxicology, Karl Franzens University Graz, Graz, Austria
    Br J Pharmacol 155:170-84. 2008
    ..Finally, we emphasize some of the most intriguing, in our opinion, unresolved puzzles of GTN pharmacology that urgently need to be addressed in future studies...
  4. doi request reprint Inactivation of soluble guanylate cyclase by stoichiometric S-nitrosation
    Bernd Mayer
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Mol Pharmacol 75:886-91. 2009
    ....
  5. pmc Characterization of the East Asian variant of aldehyde dehydrogenase-2: bioactivation of nitroglycerin and effects of Alda-1
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, 8010 Graz, Austria
    J Biol Chem 285:943-52. 2010
    ..In addition, our data revealed an unexpected discrepancy between GTN reductase activity and sGC activation, suggesting that GTN denitration and bioactivation may reflect independent pathways of ALDH2-catalyzed GTN biotransformation...
  6. doi request reprint Mitochondrial nitrite reduction coupled to soluble guanylate cyclase activation: lack of evidence for a role in the bioactivation of nitroglycerin
    Alexander Kollau
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Nitric Oxide 20:53-60. 2009
    ..Our data suggest that cytochrome c oxidase catalyzes reduction of nitrite to NO at low O(2) tension but argue against the involvement of this pathway in mitochondrial bioactivation of nitroglycerin...
  7. pmc Role of the general base Glu-268 in nitroglycerin bioactivation and superoxide formation by aldehyde dehydrogenase-2
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Biol Chem 284:19878-86. 2009
    ..ALDH2-catalyzed superoxide formation may essentially contribute to oxidative stress in GTN-exposed blood vessels...
  8. doi request reprint Potent inhibition of aldehyde dehydrogenase-2 by diphenyleneiodonium: focus on nitroglycerin bioactivation
    Regina Neubauer
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Austria
    Mol Pharmacol 84:407-14. 2013
    ..In addition, the data provide new evidence for the essential role of ALDH2 in GTN bioactivation and may have implications to other fields of ALDH2 research, such as hepatic ethanol metabolism and cardiac ischemia/reperfusion injury. ..
  9. pmc Effects of nitroglycerin/L-cysteine on soluble guanylate cyclase: evidence for an activation/inactivation equilibrium controlled by nitric oxide binding and haem oxidation
    Antonius C F Gorren
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Biochem J 390:625-31. 2005
    ..These results suggest that the effect of GTN/Cys is the result of an activation/inactivation equilibrium that is controlled by the rate of NO release and haem oxidation...
  10. doi request reprint Tetrahydrobiopterin protects soluble guanylate cyclase against oxidative inactivation
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 82:420-7. 2012
    ..Protection of sGC against oxidative inactivation may contribute to the known beneficial effects of BH4 in cardiovascular disorders associated with oxidative stress...
  11. doi request reprint Bioactivation of pentaerythrityl tetranitrate by mitochondrial aldehyde dehydrogenase
    Martina Griesberger
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 79:541-8. 2011
    ..The very low rate of ALDH2 inactivation, presumably as a result of low affinity of the denitration pathway, may at least partially explain why PETN does not induce vascular tolerance...
  12. doi request reprint Mechanisms underlying activation of soluble guanylate cyclase by the nitroxyl donor Angeli's salt
    Andreas Zeller
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 76:1115-22. 2009
    ..These data indicate that AS activates sGC exclusively via NO, formed either via SOD-catalyzed oxidation of HNO or through a minor AS decomposition pathway that is unmasked in the presence of HNO scavenging thiols...
  13. pmc Partially irreversible inactivation of mitochondrial aldehyde dehydrogenase by nitroglycerin
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    J Biol Chem 283:30735-44. 2008
    ..These observations are consistent with a significant role for irreversible inactivation of ALDH2 in the development of nitrate tolerance...
  14. doi request reprint Vascular bioactivation of nitroglycerin is catalyzed by cytosolic aldehyde dehydrogenase-2
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Austria
    Circ Res 110:385-93. 2012
    ..Despite having wide implications to GTN pharmacology and raising many questions that are still unresolved, mitochondrial bioactivation of GTN in blood vessels is still lacking experimental support...
  15. pmc Bioactivation of nitroglycerin by purified mitochondrial and cytosolic aldehyde dehydrogenases
    Matteo Beretta
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    J Biol Chem 283:17873-80. 2008
    ..Incubation of ALDH1 or ALDH2 with GTN resulted in sustained, chloral hydrate-sensitive formation of NO. These data may explain the coupling of ALDH2-catalyzed GTN metabolism to sGC activation in vascular smooth muscle...
  16. pmc Contribution of aldehyde dehydrogenase to mitochondrial bioactivation of nitroglycerin: evidence for the activation of purified soluble guanylate cyclase through direct formation of nitric oxide
    Alexander Kollau
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochem J 385:769-77. 2005
    ..In addition, the previous conflicting notion of the existence of a high-affinity GTN-metabolizing pathway operating in intact blood vessels but not in tissue homogenates is explained...
  17. doi request reprint Site-directed mutagenesis of aldehyde dehydrogenase-2 suggests three distinct pathways of nitroglycerin biotransformation
    M Verena Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010, Austria
    Mol Pharmacol 80:258-66. 2011
    ..Although the latter reaction accounts for less than 10% of total turnover of GTN metabolism catalyzed by wild-type ALDH2, it is most likely essential for vascular GTN bioactivation...
  18. pmc Endothelial dysfunction in adipose triglyceride lipase deficiency
    Astrid Schrammel
    Department of Pharmacology and Toxicology, University of Graz, Universitatsplatz 2, 8010 Graz, Austria Electronic address
    Biochim Biophys Acta 1841:906-17. 2014
    ..Our results reveal a hitherto unrecognized link between disturbed lipid metabolism, obesity and cardiovascular disease. ..
  19. ncbi request reprint Bioactivation of nitroglycerin by ascorbate
    Alexander Kollau
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Mol Pharmacol 72:191-6. 2007
    ..This reaction may contribute to GTN bioactivation in blood vessels under conditions of GTN tolerance and ascorbate supplementation...
  20. pmc Functional cardiac lipolysis in mice critically depends on comparative gene identification-58
    Kathrin A Zierler
    Institute of Molecular Biosciences, University of Graz, 8010 Graz, Austria
    J Biol Chem 288:9892-904. 2013
    ..In summary, this study demonstrates that functional muscle lipolysis depends on both CGI-58 and ATGL...
  21. ncbi request reprint High-pressure studies of the reaction mechanism of nitric-oxide synthase
    Antonius C F Gorren
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochim Biophys Acta 1764:578-85. 2006
    ..This exposition is followed by a summary of the results obtained thus far in high-pressure studies and of the conclusions that can be drawn from them...
  22. doi request reprint Evidence against tetrahydrobiopterin depletion of vascular tissue exposed to nitric oxide/superoxide or nitroglycerin
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 48:145-52. 2010
    ..GTN seems to trigger minor eNOS uncoupling that is unrelated to BH(4) depletion and without observable consequence on eNOS function...
  23. doi request reprint Vascular tolerance to nitroglycerin in ascorbate deficiency
    Gerald Wölkart
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Cardiovasc Res 79:304-12. 2008
    ..Using an established guinea pig model of ascorbate deficiency, we investigated whether endogenous ascorbate contributes to GTN-induced vasodilation...
  24. pmc Efficient nitrosation of glutathione by nitric oxide
    Bernd Kolesnik
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 63:51-64. 2013
    ..These observations suggest that direct aerobic nitrosation may represent a physiologically relevant pathway of nitrosothiol formation...
  25. pmc Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: reaction intermediates revealed by crystallography and mass spectrometry
    Barbara S Lang
    Department of Pharmacology and Toxicology, University of Graz, 8010 Graz, Austria
    J Biol Chem 287:38124-34. 2012
    ..Our results shed light on the molecular mechanism of the GTN denitration reaction and provide useful information on the structural requirements for high affinity binding of organic nitrates to the catalytic site of ALDH2...
  26. pmc Interaction between neuronal nitric-oxide synthase and tetrahydrobiopterin revisited: studies on the nature and mechanism of tight pterin binding
    Christian L Heine
    Department of Pharmacology and Toxicology, Institute of Pharmaceutical Sciences, Karl Franzens University Graz, A 8010, Graz, Austria
    Biochemistry 53:1284-95. 2014
    ..We propose that incubation of pterin-free nNOS with BH4 creates one tight pterin-binding site per dimer, leaving the other site unaffected, in a reaction that involves redox chemistry. ..
  27. pmc Cardiac oxidative stress in a mouse model of neutral lipid storage disease
    Astrid Schrammel
    Department of Pharmacology and Toxicology, Institute of Pharmaceutical Sciences, University of Graz, Universitatsplatz 2, 8010 Graz, Austria Electronic address
    Biochim Biophys Acta 1831:1600-8. 2013
    ..Upregulation of soluble guanylate cyclase and cardiac tetrahydrobiopterin might be regarded as counterregulatory mechanisms in cardiac ATGL deficiency. ..
  28. ncbi request reprint Inefficient spin trapping of superoxide in the presence of nitric-oxide: implications for studies on nitric-oxide synthase uncoupling
    Marc Pignitter
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 41:455-63. 2006
    ..In addition, the similar behavior of nNOS and SIN-1 provides further evidence for NO as initial product of the NOS reaction...
  29. ncbi request reprint Interference of the polyphenol epicatechin with the biological chemistry of nitric oxide- and peroxynitrite-mediated reactions
    Rüdiger Wippel
    Institut für Pharmakologie and Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochem Pharmacol 67:1285-95. 2004
    ..8 and 0.63 microM, respectively. Our data suggest that at up to 0.1 mM (-)-epicatechin preferentially inhibits NO-related nitration and oxidation reactions without affecting NO synthesis and cyclic GMP signaling...
  30. pmc Aldehyde dehydrogenase-independent bioactivation of nitroglycerin in porcine and bovine blood vessels
    Regina Neubauer
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Austria
    Biochem Pharmacol 93:440-8. 2015
    ..If present in the human vasculature, this pathway might contribute to the therapeutic effects of organic nitrates that are not metabolized by ALDH2. ..
  31. ncbi request reprint Tetrahydrobiopterin as combined electron/proton donor in nitric oxide biosynthesis: cryogenic UV-Vis and EPR detection of reaction intermediates
    Antonius C F Gorren
    Department of Pharmacology and Toxicology, Karl Franzens University Graz, Austria
    Methods Enzymol 396:456-66. 2005
    ..In this chapter, the suitability, drawbacks, and advantages of cryogenic methods are discussed...
  32. doi request reprint Scavenging of nitric oxide by hemoglobin in the tunica media of porcine coronary arteries
    Alexander Kollau
    Institute of Pharmaceutical Sciences, Department of Pharmacology and Toxicology, University of Graz, Austria
    Nitric Oxide 54:8-14. 2016
    ..Selective removal of hemoglobin-mediated scavenging unmasked stimulation of maximal NO-stimulated sGC activity by a soluble factor expressed in vascular tissue...
  33. pmc Desensitization of endothelial nitric oxide synthase by receptor agonists
    Sabine Wagner
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochem J 364:863-8. 2002
    ..In addition to the transient Ca2+ signal, desensitization of eNOS may represent a further mechanism by which endothelial cells rapidly terminate receptor-dependent NO formation...
  34. pmc Role of the ubiquitin-proteasome system in cardiac dysfunction of adipose triglyceride lipase-deficient mice
    Marion Mussbacher
    Department of Pharmacology and Toxicology, University of Graz, Universitatsplatz 2, A 8010 Graz, Austria Electronic address
    J Mol Cell Cardiol 77:11-9. 2014
    ..In summary, our data point to a hitherto unrecognized link between proteasomal function, PPARα signaling and cardiovascular disease. ..
  35. ncbi request reprint Effects of statins on nitric oxide/cGMP signaling in human umbilical vein endothelial cells
    Claudia Meda
    Department of Pharmacology and Toxicology, Karl Franzens University Graz, Univ Platz 2, A 8010 Graz, Austria
    Pharmacol Rep 62:100-12. 2010
    ..Stimulation of NO synthesis and cytotoxicity appear to share a common initial mechanism but involve distinct downstream signaling cascades that exhibit differential sensitivity to HMG-CoA reductase inhibition...
  36. pmc Formation of Nitric Oxide by Aldehyde Dehydrogenase-2 Is Necessary and Sufficient for Vascular Bioactivation of Nitroglycerin
    Marissa Opelt
    From the Institute of Pharmaceutical Sciences, Department of Pharmacology and Toxicology, University of Graz, A 8010 Graz, Austria
    J Biol Chem 291:24076-24084. 2016
    ..Formation of NO and cGMP accumulation were inhibited by ALDH inhibitors chloral hydrate and daidzin. The present study demonstrates that ALDH2-catalyzed NO formation is necessary and sufficient for GTN bioactivation in VSMC...
  37. pmc Aerobic nitric oxide-induced thiol nitrosation in the presence and absence of magnesium cations
    Bernd Kolesnik
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Free Radic Biol Med 76:286-98. 2014
    ..We propose that in cells Mg(2+)-stimulated NO/O2-induced nitrosothiol formation may be a physiologically relevant reaction...
  38. ncbi request reprint Consumption of nitric oxide by endothelial cells: evidence for the involvement of a NAD(P)H-, flavin- and heme-dependent dioxygenase reaction
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    FEBS Lett 577:199-204. 2004
    ....
  39. pmc Activation of endothelial nitric oxide synthase by the pro-apoptotic drug embelin: Striking discrepancy between nitric oxide-mediated cyclic GMP accumulation and L-citrulline formation
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Austria
    Nitric Oxide 22:281-9. 2010
    ..Our puzzling observations suggest that embelin improves coupling of endothelial NO synthesis to sGC activation through mobilization of an as yet unrecognized signaling pathway...
  40. ncbi request reprint Nitric-oxide synthase: a cytochrome P450 family foster child
    Antonius C F Gorren
    Department of Pharmacology und Toxicology, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochim Biophys Acta 1770:432-45. 2007
    ..In this review similarities and differences between NOS and cytochrome P450 are analyzed in an attempt to explain why NOS requires BH4 and why NO synthesis is not catalyzed by a member of the cytochrome P450 family...
  41. ncbi request reprint Redox role for tetrahydrobiopterin in nitric oxide synthase catalysis: low-temperature optical absorption spectral detection
    Antonius C F Gorren
    Institute for Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria
    Methods Enzymol 353:114-21. 2002
  42. pmc Cell type-specific recycling of tetrahydrobiopterin by dihydrofolate reductase explains differential effects of 7,8-dihydrobiopterin on endothelial nitric oxide synthase uncoupling
    Kurt Schmidt
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, A 8010 Graz, Austria Electronic address
    Biochem Pharmacol 90:246-53. 2014
    ..Thus, the differential regulation of eNOS uncoupling in different types of endothelial cells may be explained by striking differences in the apparent BH2 affinity of dihydrofolate reductase...
  43. ncbi request reprint Pharmacological interference with dimerization of human neuronal nitric-oxide synthase expressed in adenovirus-infected DLD-1 cells
    Hans Jörg Habisch
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 63:682-9. 2003
    ..As time-dependent inhibitors of NOS dimerization, BBS-1 and related compounds provide a promising strategy to develop a new class of selective and clinically useful NOS inhibitors...
  44. ncbi request reprint S-nitrosation of glutathione by nitric oxide, peroxynitrite, and (*)NO/O(2)(*-)
    Astrid Schrammel
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Graz, Austria
    Free Radic Biol Med 34:1078-88. 2003
    ..Our results indicate that concerted generation of (*)NO and O(2)(*)(-) may essentially contribute to nitrosative stress in inflammatory diseases...
  45. ncbi request reprint Functional characterization of Glu298Asp mutant human endothelial nitric oxide synthase purified from a yeast expression system
    Regina Golser
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Nitric Oxide 8:7-14. 2003
    ..To summarize, we obtained no evidence for altered enzyme function of the eNOS mutant that could explain endothelial dysfunction associated with the E298D polymorphism...
  46. ncbi request reprint Attenuation of myocardial ischemia/reperfusion injury in mice with myocyte-specific overexpression of endothelial nitric oxide synthase
    Friedrich Brunner
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Cardiovasc Res 57:55-62. 2003
    ..Wild-type (WT) mice served as controls...
  47. ncbi request reprint Binding of L-arginine and imidazole suggests heterogeneity of rat brain neuronal nitric oxide synthase
    Antonius C F Gorren
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochemistry 41:7819-29. 2002
    ..We propose that this heterogeneous response of nNOS toward Arg and imidazole is underlying the apparently conflicting results reported in the literature...
  48. pmc TRPC3 contributes to regulation of cardiac contractility and arrhythmogenesis by dynamic interaction with NCX1
    Bernhard Doleschal
    Institute of Pharmaceutical Sciences, University of Graz, Graz, Austria
    Cardiovasc Res 106:163-73. 2015
    ..TRPC3 is a non-selective cation channel, which forms a Ca2+ entry pathway involved in cardiac remodelling. Our aim was to analyse acute electrophysiological and contractile consequences of TRPC3 activation in the heart...
  49. ncbi request reprint Bioactivation of nitroglycerin--a new piece in the puzzle
    Bernd Mayer
    Institut fur Pharmakologie und Toxikologie, Universitatsplatz 2, 8010 Graz, Austria
    Angew Chem Int Ed Engl 42:388-91. 2003
  50. ncbi request reprint Lack of involvement of extracellular signal-regulated kinase (ERK) in the agonist-induced endothelial nitric oxide synthesis
    Kurt Schmidt
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    Biochem Pharmacol 63:1137-42. 2002
    ....
  51. pmc Different effects of ascorbate deprivation and classical vascular nitrate tolerance on aldehyde dehydrogenase-catalysed bioactivation of nitroglycerin
    M V Wenzl
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Br J Pharmacol 156:1248-55. 2009
    ..As relaxation to GTN is reduced but still sensitive to ALDH2 inhibitors in ascorbate deficiency, we compared the contribution of ALDH2 inactivation to GTN hyposensitivity in ascorbate deficiency and classical in vivo nitrate tolerance...
  52. pmc ATGL-mediated fat catabolism regulates cardiac mitochondrial function via PPAR-α and PGC-1
    Guenter Haemmerle
    Institute of Molecular Biosciences, University of Graz, Graz, Austria
    Nat Med 17:1076-85. 2011
    ..These findings reveal a potential treatment for the excessive cardiac lipid accumulation and often-lethal cardiomyopathy in people with neutral lipid storage disease, a disease marked by reduced or absent ATGL activity...
  53. ncbi request reprint Functional and analytical evidence for scavenging of oxygen radicals by L-arginine
    Achim Lass
    Department of Pharmacology and Toxicology, Karl Franzens Universitat Graz, Graz, Austria
    Mol Pharmacol 61:1081-8. 2002
    ..In summary, the results of this study demonstrate protective effects of L-arginine against oxygen radical-induced cardiac injury by free radical scavenging...
  54. ncbi request reprint Dityrosine formation outcompetes tyrosine nitration at low steady-state concentrations of peroxynitrite. Implications for tyrosine modification by nitric oxide/superoxide in vivo
    S Pfeiffer
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    J Biol Chem 275:6346-52. 2000
    ....
  55. ncbi request reprint Biosynthesis and action of nitric oxide in mammalian cells
    B Mayer
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Austria
    Trends Biochem Sci 22:477-81. 1997
    ..Here, we discuss progress in NO chemistry and the enzymology of NO synthases, and we will also attempt to explain its actions in the cardiovascular, nervous and immune systems...
  56. ncbi request reprint Lack of tyrosine nitration by peroxynitrite generated at physiological pH
    S Pfeiffer
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010 Graz, Austria
    J Biol Chem 273:27280-5. 1998
    ..1-2 mM), and was not enhanced by NaHCO3 (up to 20 mM), indicating that it was mediated by .NO2 rather than peroxynitrite. Our results argue against peroxynitrite produced from .NO and O-2 as a mediator of tyrosine nitration in vivo...
  57. ncbi request reprint Protein tyrosine nitration in cytokine-activated murine macrophages. Involvement of a peroxidase/nitrite pathway rather than peroxynitrite
    S Pfeiffer
    , , , A-8010 Graz, Austria
    J Biol Chem 276:34051-8. 2001
    ....
  58. ncbi request reprint The versatile and complex enzymology of nitric oxide synthase
    A C Gorren
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Universitatsplatz 2, A 8010, Graz, Austria
    Biochemistry (Mosc) 63:734-43. 1998
    ..Based on a number of recent observations, we postulate that neuronal NOS with one equivalent of BH4 per dimer (a state which may be physiologically relevant) catalyzes the concerted formation of peroxynitrite...
  59. ncbi request reprint Reaction of peroxynitrite with HEPES or MOPS results in the formation of nitric oxide donors
    K Schmidt
    Institut fur Pharmakologie und Toxikologie, Karl Franzens Universitat Graz, Austria
    Free Radic Biol Med 24:859-62. 1998
    ..These data demonstrate that certain buffer components present in cell culture media may yield artificial results in experiments with authentic ONOO-...
  60. ncbi request reprint Tracing nitrates and sulphates in river basins using isotope techniques
    L Rock
    Department of Geology and Geophysics, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada
    Water Sci Technol 53:209-17. 2006
    ..Hence, we submit that isotopic measurements can make an important contribution to the identification of nutrient and pollutant sources and to river basin management...
  61. ncbi request reprint Protein tyrosine nitration in mouse peritoneal macrophages activated in vitro and in vivo: evidence against an essential role of peroxynitrite
    S Pfeiffer
    , , A-8010 Graz, Austria
    FASEB J 15:2355-64. 2001
    ..These results argue against an essential role of peroxynitrite in protein tyrosine nitration in vivo...
  62. ncbi request reprint Isotopic assessment of sources and processes affecting sulfate and nitrate in surface water and groundwater of Luxembourg
    L Rock
    Department of Geology and Geophysics, University of Calgary, 2500 University Drive NW, Calgary, Alberta, T2N 1N4, Canada
    Isotopes Environ Health Stud 38:191-206. 2002
    ..It appears that sulfate within Luxembourg's aquatic ecosystem is mainly of lithogenic origin, whereas nitrate is often derived from anthropogenic activities...