Wolfgang F Graier

Summary

Country: Austria

Publications

  1. pmc Mitochondrial protein phosphorylation: instigator or target of lipotoxicity?
    Wolfgang F Graier
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Graz, Austria
    Trends Endocrinol Metab 20:186-93. 2009
  2. ncbi request reprint The role of mitochondria for Ca2+ refilling of the endoplasmic reticulum
    Rolland Malli
    Department of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, 8010 Graz, Austria
    J Biol Chem 280:12114-22. 2005
  3. pmc Leucine zipper EF hand-containing transmembrane protein 1 (Letm1) and uncoupling proteins 2 and 3 (UCP2/3) contribute to two distinct mitochondrial Ca2+ uptake pathways
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Molecular and Cellular Physiology Research Unit, Center of Molecular Medicine, Medical University Graz, Harrachgasse 21 III, 8010 Graz, Austria
    J Biol Chem 286:28444-55. 2011
  4. ncbi request reprint Sustained Ca2+ transfer across mitochondria is Essential for mitochondrial Ca2+ buffering, sore-operated Ca2+ entry, and Ca2+ store refilling
    Roland Malli
    Department of Medical Biochemistry and Medical Molecular Biology, University of Graz, 8010 Graz, Austria
    J Biol Chem 278:44769-79. 2003
  5. pmc Mitochondrial Ca2+ uptake 1 (MICU1) and mitochondrial ca2+ uniporter (MCU) contribute to metabolism-secretion coupling in clonal pancreatic β-cells
    Muhammad Rizwan Alam
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    J Biol Chem 287:34445-54. 2012
  6. pmc Molecularly distinct routes of mitochondrial Ca2+ uptake are activated depending on the activity of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA)
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    J Biol Chem 288:15367-79. 2013
  7. pmc Spatiotemporal correlations between cytosolic and mitochondrial Ca(2+) signals using a novel red-shifted mitochondrial targeted cameleon
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Centre of Molecular Medicine, Medical University of Graz, Graz, Austria
    PLoS ONE 7:e45917. 2012
  8. pmc The endocannabinoid N-arachidonoyl glycine (NAGly) inhibits store-operated Ca2+ entry by preventing STIM1-Orai1 interaction
    Andras T Deak
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Graz, Austria
    J Cell Sci 126:879-88. 2013
  9. pmc Uncoupling protein 3 adjusts mitochondrial Ca(2+) uptake to high and low Ca(2+) signals
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Centre of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010 Graz, Austria
    Cell Calcium 48:288-301. 2010
  10. pmc Studying mitochondrial Ca(2+) uptake - a revisit
    Claire Jean-Quartier
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, A 8010 Graz, Austria
    Mol Cell Endocrinol 353:114-27. 2012

Collaborators

Detail Information

Publications56

  1. pmc Mitochondrial protein phosphorylation: instigator or target of lipotoxicity?
    Wolfgang F Graier
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Graz, Austria
    Trends Endocrinol Metab 20:186-93. 2009
    ..Moreover, the degradation of mitochondria by mitophagy is discussed as an important landmark that leads to cellular apoptosis in lipotoxicity...
  2. ncbi request reprint The role of mitochondria for Ca2+ refilling of the endoplasmic reticulum
    Rolland Malli
    Department of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, 8010 Graz, Austria
    J Biol Chem 280:12114-22. 2005
    ....
  3. pmc Leucine zipper EF hand-containing transmembrane protein 1 (Letm1) and uncoupling proteins 2 and 3 (UCP2/3) contribute to two distinct mitochondrial Ca2+ uptake pathways
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Molecular and Cellular Physiology Research Unit, Center of Molecular Medicine, Medical University Graz, Harrachgasse 21 III, 8010 Graz, Austria
    J Biol Chem 286:28444-55. 2011
    ..Our data indicate that Letm1 and UCP2/3 independently contribute to two distinct, mitochondrial Ca(2+) uptake pathways in intact endothelial cells...
  4. ncbi request reprint Sustained Ca2+ transfer across mitochondria is Essential for mitochondrial Ca2+ buffering, sore-operated Ca2+ entry, and Ca2+ store refilling
    Roland Malli
    Department of Medical Biochemistry and Medical Molecular Biology, University of Graz, 8010 Graz, Austria
    J Biol Chem 278:44769-79. 2003
    ....
  5. pmc Mitochondrial Ca2+ uptake 1 (MICU1) and mitochondrial ca2+ uniporter (MCU) contribute to metabolism-secretion coupling in clonal pancreatic β-cells
    Muhammad Rizwan Alam
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    J Biol Chem 287:34445-54. 2012
    ..Our data highlight the crucial role of MICU1 and MCU in mitochondrial Ca(2+) uptake in pancreatic β-cells and their involvement in the positive feedback required for sustained insulin secretion...
  6. pmc Molecularly distinct routes of mitochondrial Ca2+ uptake are activated depending on the activity of the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA)
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    J Biol Chem 288:15367-79. 2013
    ..Moreover, our findings herein support the existence of distinct mitochondrial Ca(2+) uptake routes that might be essential to ensure an efficient ion transfer into mitochondria despite heterogeneous cytosolic Ca(2+) rises...
  7. pmc Spatiotemporal correlations between cytosolic and mitochondrial Ca(2+) signals using a novel red-shifted mitochondrial targeted cameleon
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Centre of Molecular Medicine, Medical University of Graz, Graz, Austria
    PLoS ONE 7:e45917. 2012
    ..Moreover, slow mitochondrial Ca(2+) extrusion and a desensitization of mitochondrial Ca(2+) uptake cause a clear difference in patterns of mitochondrial and cytosolic Ca(2+) oscillations of pancreatic beta-cells in response to D-glucose...
  8. pmc The endocannabinoid N-arachidonoyl glycine (NAGly) inhibits store-operated Ca2+ entry by preventing STIM1-Orai1 interaction
    Andras T Deak
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Graz, Austria
    J Cell Sci 126:879-88. 2013
    ..In summary, our findings revealed the STIM1-Orai1-mediated SOCE machinery as a molecular target of NAGly, which might have many implications in cell physiology...
  9. pmc Uncoupling protein 3 adjusts mitochondrial Ca(2+) uptake to high and low Ca(2+) signals
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Centre of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010 Graz, Austria
    Cell Calcium 48:288-301. 2010
    ..Our data demonstrate that distinct sites in the IML2 of UCP3 effect mitochondrial uptake of high and low Ca(2+) signals...
  10. pmc Studying mitochondrial Ca(2+) uptake - a revisit
    Claire Jean-Quartier
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, A 8010 Graz, Austria
    Mol Cell Endocrinol 353:114-27. 2012
    ..Moreover, this work highlights the necessity of the utilization of multiple technical approaches to study the complexity of mitochondrial Ca(2+) homeostasis...
  11. pmc Mitochondria and Ca(2+) signaling: old guests, new functions
    Wolfgang F Graier
    Molecular and Cellular Physiology Research Unit, MCPRU, Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Graz, Austria
    Pflugers Arch 455:375-96. 2007
    ....
  12. pmc Subplasmalemmal endoplasmic reticulum controls K(Ca) channel activity upon stimulation with a moderate histamine concentration in a human umbilical vein endothelial cell line
    Maud Frieden
    Department of Medical Biochemistry and Medical Molecular Biology, Karl Franzens University of Graz, Harrachgasse 21 III, A 8010 Graz, Austria
    J Physiol 540:73-84. 2002
    ..These findings further support our concept of the subplasmalemmal Ca(2+) control unit (SCCU) and add the local activation of Ca(2+)-activated K(+)-channels to the function of the SCCU...
  13. ncbi request reprint Free fatty acid overload attenuates Ca2+ signaling and NO production in endothelial cells
    Victor E Esenabhalu
    Department of Medical Biochemistry and Medical Molecular Biology, Karl Franzens University of Graz, Harrachgasse 21 211, A 8010 Graz, Austria
    Antioxid Redox Signal 5:147-53. 2003
    ..O(2)(-) release in FFA-loaded cells. These data indicate that FFAs significantly affect endothelial Ca(2+) signaling, eNOS activity, and.O(2)(-) release and, thus, might contribute to vascular dysfunction in atherogenesis...
  14. pmc GPR55-dependent and -independent ion signalling in response to lysophosphatidylinositol in endothelial cells
    Alexander Bondarenko
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Graz, Austria
    Br J Pharmacol 161:308-20. 2010
    ..To estimate the contribution of GPR55 to the physiological effects of LPI, the GPR55-dependent and -independent electrical responses in this cell type were investigated...
  15. pmc NAT8L (N-acetyltransferase 8-like) accelerates lipid turnover and increases energy expenditure in brown adipocytes
    Ariane R Pessentheiner
    From the Institute for Genomics and Bioinformatics, Graz University of Technology, Petergasse 14, 8010 Graz, Austria
    J Biol Chem 288:36040-51. 2013
    ..Taken together, our data show that Nat8l impacts on the brown adipogenic phenotype and suggests the existence of the NAT8L-driven NAA metabolism as a novel pathway to provide cytosolic acetyl-CoA for lipid synthesis in adipocytes. ..
  16. doi request reprint The contribution of UCP2 and UCP3 to mitochondrial Ca(2+) uptake is differentially determined by the source of supplied Ca(2+)
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Austria
    Cell Calcium 47:433-40. 2010
    ....
  17. pmc Acyl chain-dependent effect of lysophosphatidylcholine on cyclooxygenase (COX)-2 expression in endothelial cells
    Lada Brkić
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Harrachgasse 21 III, Medical University Graz, 8010 Graz, Austria
    Atherosclerosis 224:348-54. 2012
    ..In the present study, we examined the capacity of those LPC to modulate expression of cyclooxygenase (COX)-2 in vascular endothelial cells...
  18. doi request reprint Mitochondrial Ca2+ uptake and not mitochondrial motility is required for STIM1-Orai1-dependent store-operated Ca2+ entry
    Shamim Naghdi
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    J Cell Sci 123:2553-64. 2010
    ....
  19. ncbi request reprint Hyperglycemic conditions affect shape and Ca2+ homeostasis of mitochondria in endothelial cells
    Jolanta Paltauf-Doburzynska
    Department of Medical Biochemistry and Medical Molecular Biology, Medical University of Graz, 8010 Graz, Austria
    J Cardiovasc Pharmacol 44:423-36. 2004
    ....
  20. ncbi request reprint T-cadherin mediates low-density lipoprotein-initiated cell proliferation via the Ca(2+)-tyrosine kinase-Erk1/2 pathway
    Dijle Kipmen-Korgun
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Graz, Austria
    J Cardiovasc Pharmacol 45:418-30. 2005
    ..Because LDL uptake was not facilitated by T-cadherin, our data suggest that T-cadherin serves as a signaling receptor for LDL that facilitates an LDL-dependent mitogenic signal in the vasculature...
  21. pmc Anandamide initiates Ca(2+) signaling via CB2 receptor linked to phospholipase C in calf pulmonary endothelial cells
    Cristina Zoratti
    Department of Medical Biochemistry and Medical Molecular Biology, Karl Franzens, University of Graz, Harrachgasse 21 III, Graz A 8010, Austria
    Br J Pharmacol 140:1351-62. 2003
    ..Moreover, the cytosolic Ca2+ elevation was accompanied by a transient Ca2+ increase in the mitochondria. Thus, in addition to its actions on smooth muscle cells, anandamide also acts as a powerful stimulus for endothelial cells...
  22. pmc ATP increases within the lumen of the endoplasmic reticulum upon intracellular Ca2+ release
    Neelanjan Vishnu
    Institute of Molecular Biology and Biochemistry, Center of Physiological Medicine, Medical University of Graz, 8010 Graz, Austria Institute of Physiological Chemistry, Center of Physiological Medicine, Medical University of Graz, 8010 Graz, Austria Precursory Research for Embryonic Science, Japan Science and Technology Agency, Tokyo 102 0075, Japan
    Mol Biol Cell 25:368-79. 2014
    ..Our data highlight a novel Ca(2+)-controlled process that supplies the ER with additional energy upon cell stimulation. ..
  23. pmc Rearrangement of MICU1 multimers for activation of MCU is solely controlled by cytosolic Ca(2.)
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010 Graz, Austria
    Sci Rep 5:15602. 2015
    ..Our experiments provide novel details about how MCU/EMRE is regulated by MICU1 and an original approach to investigate MCU/EMRE activation in intact cells. ..
  24. pmc Characterization of distinct single-channel properties of Ca²⁺ inward currents in mitochondria
    Alexander I Bondarenko
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010 Graz, Austria
    Pflugers Arch 465:997-1010. 2013
    ..Collectively, our data identified multiple conductances of Ca(2+) currents in mitoplasts isolated from HeLa cells, thus challenging the dogma of only one unique mitochondrial Ca(2+) uniporter...
  25. pmc Mitochondrial Ca2+, the secret behind the function of uncoupling proteins 2 and 3?
    Wolfgang F Graier
    Institute of Molecular Biology and Biochemistry, Molecular and Cellular Physiology Research Unit, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, Graz, Austria
    Cell Calcium 44:36-50. 2008
    ....
  26. pmc Endothelial mitochondria--less respiration, more integration
    Lukas N Groschner
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010, Graz, Austria
    Pflugers Arch 464:63-76. 2012
    ..In this review, we want to outline the significance of mitochondria in both physiology and pathology of the vascular endothelium...
  27. pmc UCP2 modulates single-channel properties of a MCU-dependent Ca(2+) inward current in mitochondria
    Alexander I Bondarenko
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010, Graz, Austria
    Pflugers Arch 467:2509-18. 2015
    ..These findings confirm a regulatory role of UCP2 in mitochondrial Ca(2+) uptake and identify UCP2 as a selective modulator of just one distinct MCU/EMRE-dependent mitochondrial Ca(2+) inward current...
  28. pmc Generation of Red-Shifted Cameleons for Imaging Ca²⁺ Dynamics of the Endoplasmic Reticulum
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Centre of Molecular Medicine, Medical University of Graz, Harrachgasse 21, 8010 Graz, Austria
    Sensors (Basel) 15:13052-68. 2015
    ..However, ER targeted GFP/RFP-based probes were suitable to sense ER Ca2+ in a reliable manner. With this study we increased the palette of cameleons for visualizing Ca2+ dynamics within the main intracellular Ca2+ store. ..
  29. pmc Uncoupling proteins 2 and 3 are fundamental for mitochondrial Ca2+ uniport
    Michael Trenker
    Institute of Molecular Biology and Biochemistry, Centre of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010 Graz, Austria
    Nat Cell Biol 9:445-52. 2007
    ..Moreover, the identification of proteins fundemental for mitochondrial Ca(2+) uptake expands our knowledge of the physiological role for mitochondrial Ca(2+) sequestration...
  30. pmc Cytosolic Ca2+ prevents the subplasmalemmal clustering of STIM1: an intrinsic mechanism to avoid Ca2+ overload
    Roland Malli
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Graz, Austria
    J Cell Sci 121:3133-9. 2008
    ....
  31. doi request reprint Assessment of mitochondrial Ca²⁺ uptake
    Andras T Deak
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010, Graz, Austria
    Methods Mol Biol 1264:421-39. 2015
    ..Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca(2+) concentration ([Ca(2+)]mito) in a wide range of applications...
  32. pmc Acyl chain-dependent effect of lysophosphatidylcholine on endothelium-dependent vasorelaxation
    Shailaja P Rao
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Graz, Austria
    PLoS ONE 8:e65155. 2013
    ..The potency of attenuating relaxation and the relative contribution of underlying mechanisms are strongly related to LPC acyl-chain length and degree of saturation...
  33. pmc Mitochondrial Ca(2+) uniporter (MCU)-dependent and MCU-independent Ca(2+) channels coexist in the inner mitochondrial membrane
    Alexander I Bondarenko
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010, Graz, Austria
    Pflugers Arch 466:1411-20. 2014
    ..These data reveal i-MCC as MCU-dependent current while xl-MCC and b-MCC seem to be rather MCU-independent, thus, pointing to the engagement of at least two molecularly distinct mitochondrial Ca(2+) channels. ..
  34. pmc IP3-mediated STIM1 oligomerization requires intact mitochondrial Ca2+ uptake
    Andras T Deak
    The Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    J Cell Sci 127:2944-55. 2014
    ..Our findings show a stimulus-specific contribution of mitochondrial Ca(2+) uptake to the SOCE machinery, likely through a role in shaping cytosolic Ca(2+) micro-domains. ..
  35. pmc The GPR55 agonist lysophosphatidylinositol directly activates intermediate-conductance Ca2+ -activated K+ channels
    Alexander I Bondarenko
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, Graz, Austria
    Pflugers Arch 462:245-55. 2011
    ....
  36. pmc Inhibition of autophagy rescues palmitic acid-induced necroptosis of endothelial cells
    Muhammad Jadoon Khan
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    J Biol Chem 287:21110-20. 2012
    ..In summary, we introduce an unexpected mechanism of lipotoxicity in endothelial cells and provide several novel strategies to counteract the lipotoxic signaling of PA...
  37. pmc Monoglyceride lipase deficiency modulates endocannabinoid signaling and improves plaque stability in ApoE-knockout mice
    Nemanja Vujić
    Institute of Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria
    Atherosclerosis 244:9-21. 2016
    ..Thus, we speculated that in the absence of MGL, increased 2-AG concentrations mediate CB2R signaling in immune cells to modulate inflammatory responses, thereby affecting the development of atherosclerosis...
  38. pmc Active autophagy but not lipophagy in macrophages with defective lipolysis
    Madeleine Goeritzer
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21, 8010 Graz, Austria
    Biochim Biophys Acta 1851:1304-16. 2015
    ..We conclude that autophagy of proteins and cell organelles but not of LDs is active as a compensatory mechanism to circumvent and balance the reduced availability of energy substrates in A0H0 macrophages. ..
  39. pmc Mitochondria maintain maturation and secretion of lipoprotein lipase in the endoplasmic reticulum
    Karin Osibow
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, Harrachgasse 21 III, 8010 Graz, Austria
    Biochem J 396:173-82. 2006
    ..Since Ca2+ entry and mitochondrial Ca2+ homoeostasis are crucial for continuous Ca2+-dependent protein maturation in the ER, their pathological alterations may result in dysfunctional protein folding...
  40. pmc Mitochondrial Ca2+ channels: Great unknowns with important functions
    Roland Malli
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, 8010 Graz, Austria
    FEBS Lett 584:1942-7. 2010
    ..This mini-review revisits previous research on the mechanisms of mitochondrial Ca(2+) uptake and aligns them with most recent findings...
  41. pmc Nitric oxide inhibits capacitative Ca2+ entry by suppression of mitochondrial Ca2+ handling
    Baskaran Thyagarajan
    Department of Pharmacology and Toxicology, Karl Franzens University Graz, A 8010 Graz, Austria
    Br J Pharmacol 137:821-30. 2002
    ..6. Our data demonstrate that NO effectively inhibits CCE cells by cGMP-independent suppression of mitochondrial function. We suggest disruption of local Ca(2+) handling by mitochondria as a key mechanism of NO induced suppression of CCE...
  42. pmc Docosahexaenoic acid-induced unfolded protein response, cell cycle arrest, and apoptosis in vascular smooth muscle cells are triggered by Ca²⁺-dependent induction of oxidative stress
    Slaven Crnkovic
    Institute of Molecular Biology and Biochemistry, Center for Molecular Medicine, Austria
    Free Radic Biol Med 52:1786-95. 2012
    ..We conclude that Ca(2+)-dependent oxidative stress is the central and initial event responsible for induction of UPR, cell cycle arrest, and apoptosis in DHA-treated hPASMCs...
  43. pmc The GPR55 agonist lysophosphatidylinositol acts as an intracellular messenger and bidirectionally modulates Ca2+ -activated large-conductance K+ channels in endothelial cells
    Alexander I Bondarenko
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, Graz, Austria
    Pflugers Arch 461:177-89. 2011
    ..We conclude that LPI via this mechanism serves as an important modulator of endothelial electrical responses to cell stimulation...
  44. pmc Integrin clustering enables anandamide-induced Ca2+ signaling in endothelial cells via GPR55 by protection against CB1-receptor-triggered repression
    Markus Waldeck-Weiermair
    Institute of Molecular Biology and Biochemistry, Medical University Graz, Graz, A8010, Austria
    J Cell Sci 121:1704-17. 2008
    ..Altogether, these data demonstrate that the physiological effects of anandamide on endothelial cells depend on the status of integrin clustering...
  45. ncbi request reprint Cholesterol- and caveolin-rich membrane domains are essential for phospholipase A2-dependent EDHF formation
    Annarita Graziani
    Department of Pharmacology and Toxicology, Karl Franzens University, Graz, Austria
    Cardiovasc Res 64:234-42. 2004
    ..The present study was designed to investigate the role of these signaling platforms in the generation of endothelial-derived hyperpolarizing factor (EDHF)...
  46. ncbi request reprint Functional analysis of histamine receptor subtypes involved in endothelium-mediated relaxation of the human uterine artery
    Michaela M Spitaler
    Department of Medical Biochemistry and Medical Molecular Biology, Karl Franzens University of Graz, Graz, Austria
    Clin Exp Pharmacol Physiol 29:711-6. 2002
    ..9. Finally, endothelial cells can be isolated and cultured from the human uterine artery and maintain their reactivity to histamine in culture...
  47. pmc Oleoyl-lysophosphatidylcholine limits endothelial nitric oxide bioavailability by induction of reactive oxygen species
    Andrijana Kozina
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, 8010 Graz, Austria
    PLoS ONE 9:e113443. 2014
    ..We conclude that LPC 18:1 induces eNOS uncoupling and unspecific superoxide production. This results in NO scavenging by ROS, a limited endothelial NO bioavailability and impaired vascular function. ..
  48. pmc Tissue-specific expression of human lipoprotein lipase in the vascular system affects vascular reactivity in transgenic mice
    Victor E Esenabhalu
    Department of Medical Biochemistry and Medical Molecular Biology, Karl Franzens University of Graz, Harrachgasse 21 III, A 8010 Graz, Austria
    Br J Pharmacol 135:143-54. 2002
    ..Thus, vascular LPL activity might represent a primary risk factor for atherosclerosis independently from cholesterol/LDL levels...
  49. pmc N-Arachidonoyl glycine suppresses Na⁺/Ca²⁺ exchanger-mediated Ca²⁺ entry into endothelial cells and activates BK(Ca) channels independently of GPCRs
    Alexander I Bondarenko
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Graz, Austria
    Br J Pharmacol 169:933-48. 2013
    ..N-Arachidonoyl glycine (NAGly) is a lipoamino acid with vasorelaxant properties. We aimed to explore the mechanisms of NAGly's action on unstimulated and agonist-stimulated endothelial cells...
  50. doi request reprint Metabolism-secretion coupling and mitochondrial calcium activities in clonal pancreatic β-cells
    Lukas N Groschner
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Graz, Austria
    Vitam Horm 95:63-86. 2014
    ..The mitochondrial Ca²⁺ uptake machinery plays a fundamental role in this feed-forward mechanism that guarantees sustained insulin secretion and, thus, represents a promising therapeutic target for type 2 diabetes...
  51. pmc Development of novel FP-based probes for live-cell imaging of nitric oxide dynamics
    Emrah Eroglu
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21 III, 8010 Graz, Austria
    Nat Commun 7:10623. 2016
    ..Moreover, targeting of the probes was used to detect signals within mitochondria. The geNOps are useful new tools to further investigate and understand the complex patterns of signalling on the single (sub)cellular level. ..
  52. pmc Deletion of CGI-58 or adipose triglyceride lipase differently affects macrophage function and atherosclerosis
    Madeleine Goeritzer
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Graz, Austria
    J Lipid Res 55:2562-75. 2014
    ..These results suggest that macrophage function and atherosclerosis susceptibility differ fundamentally in these two animal models with disturbed TG catabolism, showing a more severe phenotype by ATGL deficiency. ..
  53. pmc Characterization of rat serum amyloid A4 (SAA4): a novel member of the SAA superfamily
    Christine Rossmann
    Institute of Molecular Biology and Biochemistry, Medical University of Graz, Graz, Austria
    Biochem Biophys Res Commun 450:1643-9. 2014
    ..In line with sequence prediction a 14-kDa non-glycosylated SAA4 protein is abundantly expressed in rat liver. Fluorescence microscopy revealed predominant localization of rSAA4-GFP-tagged fusion protein in the ER. ..
  54. pmc miR-206 controls LXRα expression and promotes LXR-mediated cholesterol efflux in macrophages
    Manjula Vinod
    Institute of Molecular Biology and Biochemistry, Medical University of Graz, Austria
    Biochim Biophys Acta 1841:827-35. 2014
    ..We therefore propose a feed-back loop between miR-206 and LXRα that might be part of an LXR auto-regulatory mechanism to fine tune LXR activity. ..
  55. pmc A new type of non-Ca2+-buffering Apo(a)-based fluorescent indicator for intraluminal Ca2+ in the endoplasmic reticulum
    Karin Osibow
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University Graz, 8010 Graz, Austria
    J Biol Chem 281:5017-25. 2006
    ....
  56. pmc Triacylglycerol accumulation activates the mitochondrial apoptosis pathway in macrophages
    Elma Aflaki
    Institute of Molecular Biology and Biochemistry, Center of Molecular Medicine, Medical University of Graz, Harrachgasse 21, 8010 Graz, Austria
    J Biol Chem 286:7418-28. 2011
    ..Results obtained during the present study define a novel pathway linking intracellular TG accumulation to mitochondrial dysfunction and programmed cell death in macrophages...