Alice Vrielink

Summary

Affiliation: University of Western Australia
Country: Australia

Publications

  1. pmc Ligand recognition by the TPR domain of the import factor Toc64 from Arabidopsis thaliana
    Rashmi Panigrahi
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, Australia
    PLoS ONE 8:e83461. 2013
  2. doi request reprint Cholesterol oxidase: biochemistry and structural features
    Alice Vrielink
    School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley, Australia
    FEBS J 276:6826-43. 2009
  3. doi request reprint Cholesterol oxidase: structure and function
    Alice Vrielink
    School of Biomedical Biomolecular and Chemical Sciences, University of Western Australia, Crawley, WA, 6009, Australia
    Subcell Biochem 51:137-58. 2010
  4. doi request reprint The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein
    Amanda J Blythe
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, 6009, Australia
    Protein Sci 25:1710-21. 2016
  5. pmc An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase
    Emily Golden
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, 6009 Australia
    Sci Rep 7:40517. 2017
  6. pmc The role of oxidoreductases in determining the function of the neisserial lipid A phosphoethanolamine transferase required for resistance to polymyxin
    Susannah Piek
    School of Pathology and Laboratory Medicine, and The Marshall Center for Infectious Diseases, Research and Training, University of Western Australia, Perth, Western Australia, Australia
    PLoS ONE 9:e106513. 2014
  7. doi request reprint Mechanism of the dehydrogenase reaction of DmpFG and analysis of inter-subunit channeling efficiency and thermodynamic parameters in the overall reaction
    Natalie E Smith
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, WA 6009, Australia
    Int J Biochem Cell Biol 45:1878-85. 2013
  8. doi request reprint The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin
    Christopher Wanty
    School of Chemistry and Biochemistry, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
    J Mol Biol 425:3389-402. 2013
  9. doi request reprint Production and characterization of recombinant perdeuterated cholesterol oxidase
    Emily Golden
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, WA 6009, Australia
    Anal Biochem 485:102-8. 2015
  10. doi request reprint Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity
    Emily Golden
    School of Chemistry and Biochemistry, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
    Biochem J 451:217-26. 2013

Collaborators

Detail Information

Publications13

  1. pmc Ligand recognition by the TPR domain of the import factor Toc64 from Arabidopsis thaliana
    Rashmi Panigrahi
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, Australia
    PLoS ONE 8:e83461. 2013
    ..Our findings from ITC and molecular dynamics studies suggest that AtToc64_TPR does not discriminate between C-termini peptides of Hsp70 and Hsp90. ..
  2. doi request reprint Cholesterol oxidase: biochemistry and structural features
    Alice Vrielink
    School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, Crawley, Australia
    FEBS J 276:6826-43. 2009
    ..The presence of a molecular tunnel that is proposed to serve in the access of dioxygen to the active site and mechanisms of its control by a 'gate' formed by amino acid residues are highlighted...
  3. doi request reprint Cholesterol oxidase: structure and function
    Alice Vrielink
    School of Biomedical Biomolecular and Chemical Sciences, University of Western Australia, Crawley, WA, 6009, Australia
    Subcell Biochem 51:137-58. 2010
    ..In this review the biochemical, structural, kinetic and mechanistic features of the enzyme are discussed...
  4. doi request reprint The yeast transcription elongation factor Spt4/5 is a sequence-specific RNA binding protein
    Amanda J Blythe
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, 6009, Australia
    Protein Sci 25:1710-21. 2016
    ..These findings attribute a new function to a domain of Spt4/5 that associates directly with RNAPII, making significant steps towards elucidating the mechanism behind transcriptional control by Spt4/5. ..
  5. pmc An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase
    Emily Golden
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia, 6009 Australia
    Sci Rep 7:40517. 2017
    ..We propose a novel second-order partial-charge interaction network which enables the correct orientation of the hydride receiving orbital of N5. The implications of these observations for flavin mediated redox chemistry are discussed...
  6. pmc The role of oxidoreductases in determining the function of the neisserial lipid A phosphoethanolamine transferase required for resistance to polymyxin
    Susannah Piek
    School of Pathology and Laboratory Medicine, and The Marshall Center for Infectious Diseases, Research and Training, University of Western Australia, Perth, Western Australia, Australia
    PLoS ONE 9:e106513. 2014
    ..This effect was more pronounced when neisserial LptA was expressed in E. coli than in N. meningitidis and may reflect that other factors in the neisserial periplasm have a role in LptA stability. ..
  7. doi request reprint Mechanism of the dehydrogenase reaction of DmpFG and analysis of inter-subunit channeling efficiency and thermodynamic parameters in the overall reaction
    Natalie E Smith
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, WA 6009, Australia
    Int J Biochem Cell Biol 45:1878-85. 2013
    ..This supports the concept of allosteric structural communication between the two enzymes to coordinate their activities...
  8. doi request reprint The structure of the neisserial lipooligosaccharide phosphoethanolamine transferase A (LptA) required for resistance to polymyxin
    Christopher Wanty
    School of Chemistry and Biochemistry, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
    J Mol Biol 425:3389-402. 2013
    ..The structure reveals the protein components needed for the transferase chemistry; however, substrate-binding regions are not evident and are likely to reside in the transmembrane domain of the protein. ..
  9. doi request reprint Production and characterization of recombinant perdeuterated cholesterol oxidase
    Emily Golden
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, WA 6009, Australia
    Anal Biochem 485:102-8. 2015
    ..Together, structural and functional studies indicate that the perdeuterated protein is suitable for structural studies by neutron crystallography directed at understanding the role of hydrogen atoms in enzyme catalysis. ..
  10. doi request reprint Structure of a class III engineered cephalosporin acylase: comparisons with class I acylase and implications for differences in substrate specificity and catalytic activity
    Emily Golden
    School of Chemistry and Biochemistry, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
    Biochem J 451:217-26. 2013
    ..A view of the transition state configuration of the enzyme provides important insights into the mechanism of substrate binding and catalysis...
  11. doi request reprint Detergents in Membrane Protein Purification and Crystallisation
    Anandhi Anandan
    School of Chemistry and Biochemistry, University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia
    Adv Exp Med Biol 922:13-28. 2016
    ..The last section will briefly cover the types of detergent and their properties focusing on custom designed detergents for membrane protein studies. ..
  12. doi request reprint High-resolution structures of cholesterol oxidase in the reduced state provide insights into redox stabilization
    Emily Golden
    School of Chemistry and Biochemistry, University of Western Australia, Crawley, Western Australia 6009, Australia
    Acta Crystallogr D Biol Crystallogr 70:3155-66. 2014
    ..These calculations suggest that strong hydrogen-bond interactions between Gly120 and the flavin N5 centre may play an important role in these structural features. ..
  13. doi request reprint Ubiquitin fusion constructs allow the expression and purification of multi-KOW domain complexes of the Saccharomyces cerevisiae transcription elongation factor Spt4/5
    Amanda Blythe
    School of Chemistry and Biochemistry, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia
    Protein Expr Purif 100:54-60. 2014
    ..These expression constructs encompass a larger region of Spt5 than has previously been reported, and will provide the opportunity to elucidate the biological function of the multi-KOW containing Spt5. ..