J A Trapani

Summary

Affiliation: University of Melbourne
Country: Australia

Publications

  1. ncbi request reprint CTL granules: evolution of vesicles essential for combating virus infections
    J A Trapani
    Austin Research Institute, Studley Road, Heidelberg 3084, Australia
    Immunol Today 20:351-6. 1999
  2. ncbi request reprint Efficient nuclear targeting of granzyme B and the nuclear consequences of apoptosis induced by granzyme B and perforin are caspase-dependent, but cell death is caspase-independent
    J A Trapani
    The John Connell Laboratory, The Austin Research Institute, Studley Road, Heidelberg 3084, Australia
    J Biol Chem 273:27934-8. 1998
  3. ncbi request reprint Isotypic variants of the interferon-inducible transcriptional repressor IFI 16 arise through differential mRNA splicing
    R W Johnstone
    The John Connell Laboratory, The Austin Research Institute, Heidelberg, Australia
    Biochemistry 37:11924-31. 1998
  4. ncbi request reprint Adoptive transfer: the role of perforin in mouse cytotoxic T lymphocyte rejection of human tumor xenografts in vivo
    M J Smyth
    Cellular Cytotoxicity Laboratory, The Austin Research Institute, Heidelberg, Victoria, Australia
    Xenotransplantation 5:146-53. 1998
  5. ncbi request reprint Proapoptotic functions of cytotoxic lymphocyte granule constituents in vitro and in vivo
    J A Trapani
    The Research Division, The John Connell Laboratory, Peter MacCallum Cancer Institute, St Andrew s Place, Austin Research Institute, Heidelberg, 3084, Australia au
    Curr Opin Immunol 12:323-9. 2000
  6. ncbi request reprint Anti-viral strategies of cytotoxic T lymphocytes are manifested through a variety of granule-bound pathways of apoptosis induction
    K M Edwards
    John Connell Laboratory, Austin Research Institute, Heidelberg, Victoria, Australia
    Immunol Cell Biol 77:76-89. 1999
  7. ncbi request reprint Hypothesis: cytotoxic lymphocyte granule serine proteases activate target cell endonucleases to trigger apoptosis
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Heidelberg, Victoria, Australia
    Clin Exp Pharmacol Physiol 21:67-70. 1994
  8. ncbi request reprint Redirected perforin-dependent lysis of colon carcinoma by ex vivo genetically engineered CTL
    P K Darcy
    Cellular Cytotoxicity Laboratory, The Austin Research Institute, Heidelberg, Victoria, Australia
    J Immunol 164:3705-12. 2000
  9. ncbi request reprint IFI 16 gene encodes a nuclear protein whose expression is induced by interferons in human myeloid leukaemia cell lines
    M J Dawson
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Australia
    J Cell Biochem 57:39-51. 1995
  10. ncbi request reprint Perforin-dependent nuclear entry of granzyme B precedes apoptosis, and is not a consequence of nuclear membrane dysfunction
    J A Trapani
    Cellular Cytotoxicity Laboratory, The Austin Research Institute, Studley Road, Heidelberg, 3084, Australia
    Cell Death Differ 5:488-96. 1998

Collaborators

Detail Information

Publications49

  1. ncbi request reprint CTL granules: evolution of vesicles essential for combating virus infections
    J A Trapani
    Austin Research Institute, Studley Road, Heidelberg 3084, Australia
    Immunol Today 20:351-6. 1999
    ..Here, Joseph Trapani and colleagues argue that CTL cytolytic granules are the principal apoptotic means of eliminating viruses and possess multiple independent mechanisms to counter the viral anti-apoptotic machinery...
  2. ncbi request reprint Efficient nuclear targeting of granzyme B and the nuclear consequences of apoptosis induced by granzyme B and perforin are caspase-dependent, but cell death is caspase-independent
    J A Trapani
    The John Connell Laboratory, The Austin Research Institute, Studley Road, Heidelberg 3084, Australia
    J Biol Chem 273:27934-8. 1998
    ..We conclude that cell death in response to perforin and granzyme B does not require caspase activation and still proceeds efficiently through non-nuclear pathways when nuclear substrate cleavage is inhibited...
  3. ncbi request reprint Isotypic variants of the interferon-inducible transcriptional repressor IFI 16 arise through differential mRNA splicing
    R W Johnstone
    The John Connell Laboratory, The Austin Research Institute, Heidelberg, Australia
    Biochemistry 37:11924-31. 1998
    ..Finally, IFI 16 isoforms can homo- and heterodimerize, and we have mapped the dimerization domain to the amino terminus which contains an imperfect leucine zipper domain...
  4. ncbi request reprint Adoptive transfer: the role of perforin in mouse cytotoxic T lymphocyte rejection of human tumor xenografts in vivo
    M J Smyth
    Cellular Cytotoxicity Laboratory, The Austin Research Institute, Heidelberg, Victoria, Australia
    Xenotransplantation 5:146-53. 1998
    ..These data indicate that perforin, and hence direct cytotoxicity, plays a key role in the ability of adoptively transferred CD8+ cytotoxic T lymphocytes to eradicate established xenografts...
  5. ncbi request reprint Proapoptotic functions of cytotoxic lymphocyte granule constituents in vitro and in vivo
    J A Trapani
    The Research Division, The John Connell Laboratory, Peter MacCallum Cancer Institute, St Andrew s Place, Austin Research Institute, Heidelberg, 3084, Australia au
    Curr Opin Immunol 12:323-9. 2000
    ....
  6. ncbi request reprint Anti-viral strategies of cytotoxic T lymphocytes are manifested through a variety of granule-bound pathways of apoptosis induction
    K M Edwards
    John Connell Laboratory, Austin Research Institute, Heidelberg, Victoria, Australia
    Immunol Cell Biol 77:76-89. 1999
    ..The fate of such a cell therefore reflects the balance of pro-apoptotic (immune) and anti-apoptotic (viral) strategies that have developed over eons of evolutionary time...
  7. ncbi request reprint Hypothesis: cytotoxic lymphocyte granule serine proteases activate target cell endonucleases to trigger apoptosis
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Heidelberg, Victoria, Australia
    Clin Exp Pharmacol Physiol 21:67-70. 1994
    ..In cytotoxic effectors, these nuclear substrates are normally protected from granzymes by endogenous inhibitors...
  8. ncbi request reprint Redirected perforin-dependent lysis of colon carcinoma by ex vivo genetically engineered CTL
    P K Darcy
    Cellular Cytotoxicity Laboratory, The Austin Research Institute, Heidelberg, Victoria, Australia
    J Immunol 164:3705-12. 2000
    ..This series of experiments has revealed the utility of scFv anti-CEA chimeras in providing mouse T cells the capacity to reject colon carcinoma in an Ag- and perforin-specific manner...
  9. ncbi request reprint IFI 16 gene encodes a nuclear protein whose expression is induced by interferons in human myeloid leukaemia cell lines
    M J Dawson
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Australia
    J Cell Biochem 57:39-51. 1995
    ..Therefore, IFI 16 and MNDA are members of a novel family of human DNA-binding proteins whose expression is associated with myeloid cell differentiation induced by cytokines and chemical agents...
  10. ncbi request reprint Perforin-dependent nuclear entry of granzyme B precedes apoptosis, and is not a consequence of nuclear membrane dysfunction
    J A Trapani
    Cellular Cytotoxicity Laboratory, The Austin Research Institute, Studley Road, Heidelberg, 3084, Australia
    Cell Death Differ 5:488-96. 1998
    ..Rather, a perforin-dependent signal results in a redistribution of grB from the cytoplasm to the nucleus, where it may contribute to the nuclear changes associated with apoptosis...
  11. ncbi request reprint The human cytotoxic T cell granule serine protease granzyme H has chymotrypsin-like (chymase) activity and is taken up into cytoplasmic vesicles reminiscent of granzyme B-containing endosomes
    K M Edwards
    The John Connell Laboratory, The Austin Research Institute, Studley Road, Heidelberg, 3084, Australia
    J Biol Chem 274:30468-73. 1999
    ..This suggests a hitherto unsuspected intracellular function for granzyme H...
  12. ncbi request reprint The IFN-inducible nucleoprotein IFI 16 is expressed in cells of the monocyte lineage, but is rapidly and markedly down-regulated in other myeloid precursor populations
    M J Dawson
    Cellular Cytotoxicity Laboratory, The Austin Research Institute, Heidelberg, Australia
    J Leukoc Biol 64:546-54. 1998
    ..This differential expression of IFI 16 in myeloid precursor subpopulations and its perceived molecular properties are consistent with a possible role in regulating myelopoiesis...
  13. ncbi request reprint Met-ase: cloning and distinct chromosomal location of a serine protease preferentially expressed in human natural killer cells
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
    J Immunol 151:6195-205. 1993
    ..The gene for the Hu-Met-1 serine protease is located on chromosome 19, which distinguishes it from any other member of the human granzyme family...
  14. ncbi request reprint The closely linked genes encoding the myeloid nuclear differentiation antigen (MNDA) and IFI16 exhibit contrasting haemopoietic expression
    M J Dawson
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Vic, Australia
    Immunogenetics 41:40-3. 1995
  15. ncbi request reprint Granzymes: a variety of serine protease specificities encoded by genetically distinct subfamilies
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
    J Leukoc Biol 60:555-62. 1996
    ....
  16. ncbi request reprint Genomic organization of IFI16, an interferon-inducible gene whose expression is associated with human myeloid cell differentiation: correlation of predicted protein domains with exon organization
    J A Trapani
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Vic, Australia
    Immunogenetics 40:415-24. 1994
    ..abstract truncated at 400 words)..
  17. ncbi request reprint Cloning and characterization of a novel NK cell-specific serine protease gene and its functional 5'-flanking sequences
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
    Immunogenetics 42:101-11. 1995
    ..These data are consistent with the NK cell-specific expression of RNK-Met-1 and suggest the potential utility of this gene promoter in the development of transgene models of NK cell biology in vivo...
  18. ncbi request reprint The human interferon-inducible protein, IFI 16, is a repressor of transcription
    R W Johnstone
    The Austin Research Institute, Austin Hospital, Studley Road, Heidelberg 3084, Victoria, Australia
    J Biol Chem 273:17172-7. 1998
    ..Thus, IFI 16 is a transcriptional repressor, with a modular structure typical of many known transcription regulators...
  19. pmc Differential tumor surveillance by natural killer (NK) and NKT cells
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin and Repatriation Medical Centre, Heidelberg, 3084 Victoria, Australia
    J Exp Med 191:661-8. 2000
    ..This is the first description of an antitumor function for NKT cells in the absence of exogenously administered potent stimulators such as IL-12 or alpha-galactosylceramide...
  20. pmc Perforin-mediated cytotoxicity is critical for surveillance of spontaneous lymphoma
    M J Smyth
    Austin Research Institute, Heidelberg, 3084, Victoria, Australia
    J Exp Med 192:755-60. 2000
    ..This study is the first that implicates direct cytotoxicity by lymphocytes in regulating lymphomagenesis...
  21. ncbi request reprint Granzymes: exogenous proteinases that induce target cell apoptosis
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Heidelberg, Australia
    Immunol Today 16:202-6. 1995
    ....
  22. ncbi request reprint A novel gene constitutively expressed in human lymphoid cells is inducible with interferon-gamma in myeloid cells
    J A Trapani
    Austin Research Institute, Austin Hospital, Heidelberg, Australia
    Immunogenetics 36:369-76. 1992
    ..DNA blotting indicated that, in contrast to the mouse, IFI 16 is present as a single copy gene in the human genome...
  23. pmc Production of anti-breast cancer monoclonal antibodies using a glutathione-S-transferase-MUC1 bacterial fusion protein
    V Apostolopoulos
    Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
    Br J Cancer 67:713-20. 1993
    ..The use of fusion proteins provides another means of immunisation to produce anti-tumour antibodies...
  24. ncbi request reprint Purification and cloning of a novel serine protease, RNK-Met-1, from the granules of a rat natural killer cell leukemia
    M J Smyth
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Austin Hospital, Heidelberg, Victoria, Australia
    J Biol Chem 267:24418-25. 1992
    ..These data indicate that RNK-Met-1 is a serine protease with unique activity that is expressed in the granules of large granular lymphocytes...
  25. ncbi request reprint Functional interaction between p53 and the interferon-inducible nucleoprotein IFI 16
    R W Johnstone
    The Peter MacCallum Cancer Institute, Cancer Immunology Division, East Melbourne, Victoria, Australia
    Oncogene 19:6033-42. 2000
    ..These findings demonstrate a possible link between gene induction following interferon stimulation and p53-mediated cellular events...
  26. doi request reprint Blocking granule-mediated death by primary human NK cells requires both protection of mitochondria and inhibition of caspase activity
    K A Sedelies
    Cancer Cell Death Laboratory, Cancer Immunology Program, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett Street, Melbourne, Victoria 8006, Australia
    Cell Death Differ 15:708-17. 2008
    ....
  27. ncbi request reprint CTL: Caspases Terminate Life, but that's not the whole story
    N J Waterhouse
    Cancer Immunology Laboratory, Research Division, Peter MacCallum Cancer Institute, St Andrews Place, East Melbourne, Australia
    Tissue Antigens 59:175-83. 2002
    ..Here we will discuss the mechanisms by which these stellar killers achieve their goal...
  28. pmc Novel properties of the protein kinase CK2-site-regulated nuclear- localization sequence of the interferon-induced nuclear factor IFI 16
    L J Briggs
    Nuclear Signalling Laboratory, Division for Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, P O Box 334, Canberra City, A C T 2601, Australia
    Biochem J 353:69-77. 2001
    ....
  29. ncbi request reprint Critical role of the transcription factor AP-1 for the constitutive and interferon-induced expression of IFI 16
    C J P Clarke
    Trescowthick Research Laboratories, Peter MacCallum Cancer Institute, St Andrews Place, East Melbourne, Victoria 3002, Australia
    J Cell Biochem 89:80-93. 2003
    ..These experiments define the transcriptional mechanisms of IFI 16 gene regulation and provide evidence suggesting that AP-1 activation may be an important event in IFN signaling...
  30. ncbi request reprint Filamin (280-kDa actin-binding protein) is a caspase substrate and is also cleaved directly by the cytotoxic T lymphocyte protease granzyme B during apoptosis
    K A Browne
    Cancer Immunology Laboratory, Peter MacCallum Cancer Institute, Locked Bag 1, A Beckett Street, Melbourne 8006, Australia
    J Biol Chem 275:39262-6. 2000
    ..Thus, filamin is a functionally important substrate for granzyme B, as its cleavage may account at least partly for caspase-independent cell death mediated by the granzyme...
  31. ncbi request reprint Functional dissociation of DeltaPsim and cytochrome c release defines the contribution of mitochondria upstream of caspase activation during granzyme B-induced apoptosis
    N J Waterhouse
    Cancer Cell Death, Peter MacCallum Cancer Centre, Locked Bag 1, A Beckett Street, Melbourne, Victoria 8006, Australia
    Cell Death Differ 13:607-18. 2006
    ..This functional dissociation of cytochrome c and SMAC release from loss of DeltaPsim demonstrates the essential contribution of Bid upstream of caspase activation during granzyme B-induced apoptosis...
  32. ncbi request reprint Novel putative promoter/enhancer sequences are shared by the mouse and human perforin (Pfp) genes
    J A Trapani
    Department of Pathology, University of Melbourne, Parkville, Australia
    Tissue Antigens 36:228-34. 1990
    ....
  33. pmc Genomic organization of the mouse pore-forming protein (perforin) gene and localization to chromosome 10. Similarities to and differences from C9
    J A Trapani
    Laboratory of Human Immunogenetics, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    J Exp Med 171:545-57. 1990
    ..Using a panel of somatic cell hybrid cell lines, Pfp has been mapped to chromosome 10...
  34. ncbi request reprint Characterization of a novel, human cytotoxic lymphocyte-specific serine protease cDNA clone (CSP-C)
    J L Klein
    Laboratory of Human Immunogenetics, Memorial Sloan Kettering Cancer Center, New York, New York
    Tissue Antigens 35:220-8. 1990
    ..It is likely that the CSP-C cDNA clone reported in this study corresponds to CGL-2...
  35. ncbi request reprint Genomic organization and chromosomal assignment for a serine protease gene (CSPB) expressed by human cytotoxic lymphocytes
    J L Klein
    Laboratory of Human Immunogenetics, Memorial Sloan Kettering Cancer Center, New York, New York 10021
    Genomics 5:110-7. 1989
    ..Using DNA blot analysis of a panel of human-rodent somatic cell lines, the human serine protease B gene has been localized to chromosome 14...
  36. pmc Molecular cloning of an inducible serine esterase gene from human cytotoxic lymphocytes
    J A Trapani
    Laboratory of Human Immunogenetics, Memorial Sloan Kettering Cancer Center, New York, NY 10021
    Proc Natl Acad Sci U S A 85:6924-8. 1988
    ..The data suggest that the protein encoded by HSE26.1 plays a role in cell-mediated cytotoxicity...
  37. ncbi request reprint The structure of the mouse lymphocyte pore-forming protein perforin
    B S Kwon
    Department of Microbiology and Immunology, Indiana Univ School of Medicine, Indianapolis 46223
    Biochem Biophys Res Commun 158:1-10. 1989
    ..Southern blot analysis of mouse genomic DNA indicated that PFP is encoded as a single-copy gene with the coding region contained within 10 kilobases of genomic DNA...
  38. ncbi request reprint Target cell apoptosis induced by cytotoxic T cells and natural killer cells involves synergy between the pore-forming protein, perforin, and the serine protease, granzyme B
    J A Trapani
    Cellular Cytotoxicity Laboratory, Austin Research Institute, Melbourne, Vic
    Aust N Z J Med 25:793-9. 1995
    ..elegans, are currently under investigation...
  39. ncbi request reprint A cytosolic granzyme B inhibitor related to the viral apoptotic regulator cytokine response modifier A is present in cytotoxic lymphocytes
    J Sun
    Department of Medicine, Monash Medical School, Box Hill Hospital, Box Hill 3128, Australia
    J Biol Chem 271:27802-9. 1996
    ..These results suggest that PI-9 serves to inactivate misdirected granzyme B following cytotoxic cell degranulation. This may explain why cytotoxic cells are not damaged by their own granzyme B during destruction of abnormal cells...
  40. ncbi request reprint A new family of 10 murine ovalbumin serpins includes two homologs of proteinase inhibitor 8 and two homologs of the granzyme B inhibitor (proteinase inhibitor 9)
    J Sun
    Department of Medicine, Monash Medical School, Clive Ward Centre, Box Hill Hospital, Box Hill 3128, Australia
    J Biol Chem 272:15434-41. 1997
    ....
  41. pmc In vitro activation of CPP32 and Mch3 by Mch4, a novel human apoptotic cysteine protease containing two FADD-like domains
    T Fernandes-Alnemri
    Center for Apoptosis Research and the Kimmel Cancer Center Institute, Jefferson Medical College, Philadelphia, PA 19107, USA
    Proc Natl Acad Sci U S A 93:7464-9. 1996
    ..The presence of the FADD-like domains in Mch4 and Mch5 suggests a role for these proteases in the Fas-apoptotic pathway. In addition, these proteases could participate in the granzyme B apoptotic pathways...
  42. ncbi request reprint Isolation and characterization of cDNA clones for mouse Ly-9
    M S Sandrin
    Molecular Immunogenetics Laboratory, Austin Research Institute, Austin Hospital Heidelberg, Victoria, Australia
    J Immunol 149:1636-41. 1992
    ..The sequence data also support the view that Ly-9 belongs to the subgroup of the Ig superfamily that includes Bcm-1, CD2, and LFA-3...
  43. ncbi request reprint Lack of apoptosis of S├ęzary cells in the circulation following oral bexarotene therapy
    S Brennand
    Division of Haematology and Medical Oncology, Peter MacCallum Cancer Centre, University of Melbourne, Australia
    Br J Dermatol 152:1199-205. 2005
    ..Apoptosis of malignant cells has been suggested as an important mechanism of the action of bexarotene in the treatment of cutaneous T-cell lymphoma (CTCL)...
  44. ncbi request reprint A new quantitative assay for cytochrome c release in apoptotic cells
    N J Waterhouse
    Cell Death Differ 10:853-5. 2003
  45. ncbi request reprint Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma
    K L DeYoung
    Laboratory of Cancer Genetics, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland 20892, USA
    Oncogene 15:453-7. 1997
    ..We have also demonstrated that, like IFI16 and MNDA, AIM2 is induced in HL60 cells by interferon gamma. Our findings support the existence of a family of genes in this region similar to the well-characterized mouse Ifi200 gene family...
  46. ncbi request reprint Isolation and nucleotide sequence of a cDNA clone encoding a novel HLA class I gene
    S Mizuno
    Laboratory of Biochemical Immunogenetics, Sloan Kettering Institute, New York 10021
    J Immunol 140:4024-30. 1988
    ..The JTW15 gene transcript was found in a broad variety of cell types and was inducible in cells treated with IFN-gamma. JTW15 is the first isolated full length cDNA clone defining a non-HLA-ABC class I gene...
  47. pmc Initiation of apoptosis by granzyme B requires direct cleavage of bid, but not direct granzyme B-mediated caspase activation
    V R Sutton
    Cancer Immunology Laboratory, Peter MacCallum Cancer Institute, Melbourne 8006, Australia
    J Exp Med 192:1403-14. 2000
    ..Overall, our results indicate that mitochondrial perturbation by Bid is necessary to achieve a lethal threshold of caspase activity and cell death due to granzyme B...
  48. ncbi request reprint The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32
    S M Srinivasula
    Center for Apoptosis Research, the Department of Biochemistry and Molecular Pharmacology, and the Kimmel Cancer Institute, Jefferson Medical College, Philadelphia, Pennsylvania 19107, USA
    J Biol Chem 271:27099-106. 1996
    ..This is the first demonstration of a protease cascade involving granzyme B, CPP32, Mch2alpha, and Mch6 and evidence that the lamin-cleaving enzyme Mch2 is a target of mature CPP32...
  49. ncbi request reprint Perforin-independent expression of granzyme B and proteinase inhibitor 9 in human testis and placenta suggests a role for granzyme B-mediated proteolysis in reproduction
    C E Hirst
    Department of Biochemistry and Molecular Biology, Monash University, Clayton, 3800, Australia
    Mol Hum Reprod 7:1133-42. 2001
    ..In the testis, graB may facilitate migration of developing germ cells, while in the placenta, it may contribute to extracellular matrix remodelling during parturition...