Norelle L Daly

Summary

Affiliation: University of Queensland
Country: Australia

Publications

  1. pmc Structure of catalytic domain of Matriptase in complex with Sunflower trypsin inhibitor-1
    Cai Yuan
    State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
    BMC Struct Biol 11:30. 2011
  2. doi request reprint Structural studies of conotoxins
    Norelle L Daly
    The University of Queensland, Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, Brisbane, QLD, Australia
    IUBMB Life 61:144-50. 2009
  3. pmc Structure and activity of alpha-conotoxin PeIA at nicotinic acetylcholine receptor subtypes and GABA(B) receptor-coupled N-type calcium channels
    Norelle L Daly
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 286:10233-7. 2011
  4. doi request reprint Design and therapeutic applications of cyclotides
    Norelle L Daly
    The University of Queensland, Institute for Molecular Bioscience, Division of Chemistry and Structural Biology, Brisbane, Queensland 4072, Australia
    Future Med Chem 1:1613-22. 2009
  5. ncbi request reprint Isolation and characterization of novel cyclotides from Viola hederaceae: solution structure and anti-HIV activity of vhl-1, a leaf-specific expressed cyclotide
    Bin Chen
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    J Biol Chem 280:22395-405. 2005
  6. pmc Phosphatidylethanolamine binding is a conserved feature of cyclotide-membrane interactions
    Sónia Troeira Henriques
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, 4072 Queensland, Australia
    J Biol Chem 287:33629-43. 2012
  7. ncbi request reprint Knots in rings. The circular knotted protein Momordica cochinchinensis trypsin inhibitor-II folds via a stable two-disulfide intermediate
    Masa Cemazar
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane 4072 QLD, Australia
    J Biol Chem 281:8224-32. 2006
  8. doi request reprint Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues
    Emma L Millard
    Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    J Biol Chem 284:4944-51. 2009
  9. doi request reprint Molecular engineering of conotoxins: the importance of loop size to alpha-conotoxin structure and function
    Ai Hua Jin
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072 Australia
    J Med Chem 51:5575-84. 2008
  10. pmc Structure of alpha-conotoxin BuIA: influences of disulfide connectivity on structural dynamics
    Ai Hua Jin
    Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane QLD, Australia
    BMC Struct Biol 7:28. 2007

Collaborators

Detail Information

Publications84

  1. pmc Structure of catalytic domain of Matriptase in complex with Sunflower trypsin inhibitor-1
    Cai Yuan
    State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou, Fujian 350002, China
    BMC Struct Biol 11:30. 2011
    ..Sunflower trypsin inhibitor-1 (SFTI-1), a cyclic peptide inhibitor originally isolated from sunflower seeds, exhibits potent inhibitory activity toward matriptase...
  2. doi request reprint Structural studies of conotoxins
    Norelle L Daly
    The University of Queensland, Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, Brisbane, QLD, Australia
    IUBMB Life 61:144-50. 2009
    ..This short article gives an overview of the structural diversity of conotoxins, and illustrates this diversity with recent selected examples of conotoxin structures...
  3. pmc Structure and activity of alpha-conotoxin PeIA at nicotinic acetylcholine receptor subtypes and GABA(B) receptor-coupled N-type calcium channels
    Norelle L Daly
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 286:10233-7. 2011
    ..Furthermore, there is probably a much more diverse range of conotoxins that target the GABA(B) receptor than currently realized...
  4. doi request reprint Design and therapeutic applications of cyclotides
    Norelle L Daly
    The University of Queensland, Institute for Molecular Bioscience, Division of Chemistry and Structural Biology, Brisbane, Queensland 4072, Australia
    Future Med Chem 1:1613-22. 2009
    ..There is a wide range of disease states that can be targeted using the cyclotide framework and the advances that have been made in the production of cyclotides will facilitate their development as pharmaceutical templates...
  5. ncbi request reprint Isolation and characterization of novel cyclotides from Viola hederaceae: solution structure and anti-HIV activity of vhl-1, a leaf-specific expressed cyclotide
    Bin Chen
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    J Biol Chem 280:22395-405. 2005
    ..Met(7) was found to be oxidized in the native form, consistent with the fact that its side chain protrudes into the solvent, occupying 7.5% of the molecular surface. vhl-1 shows anti-HIV activity with an EC(50) value of 0.87 microm...
  6. pmc Phosphatidylethanolamine binding is a conserved feature of cyclotide-membrane interactions
    Sónia Troeira Henriques
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, 4072 Queensland, Australia
    J Biol Chem 287:33629-43. 2012
    ..Knowledge of their membrane specificity has the potential to assist in the design of novel drugs based on the cyclotide framework, perhaps allowing the targeting of peptide drugs to specific cell types...
  7. ncbi request reprint Knots in rings. The circular knotted protein Momordica cochinchinensis trypsin inhibitor-II folds via a stable two-disulfide intermediate
    Masa Cemazar
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane 4072 QLD, Australia
    J Biol Chem 281:8224-32. 2006
    ..The fact that macrocyclic proteins are readily able to fold to a complex knotted structure in vitro in the absence of chaperones makes them suitable as protein engineering scaffolds that have remarkable stability...
  8. doi request reprint Inhibition of neuronal nicotinic acetylcholine receptor subtypes by alpha-Conotoxin GID and analogues
    Emma L Millard
    Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    J Biol Chem 284:4944-51. 2009
    ..Overall, our findings contribute to the understanding of the structure-activity relationships of alpha-conotoxins and may be beneficial for the ongoing attempts to exploit modulators of the neuronal nAChRs as therapeutic agents...
  9. doi request reprint Molecular engineering of conotoxins: the importance of loop size to alpha-conotoxin structure and function
    Ai Hua Jin
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072 Australia
    J Med Chem 51:5575-84. 2008
    ..Docking simulations identified several hydrogen bonds lost upon truncation that provide an explanation for the reduced affinities observed at the alpha7 nAChR and AChBP...
  10. pmc Structure of alpha-conotoxin BuIA: influences of disulfide connectivity on structural dynamics
    Ai Hua Jin
    Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane QLD, Australia
    BMC Struct Biol 7:28. 2007
    ..BuIA is the only alpha-conotoxin containing a 4/4 cysteine spacing and thus it is of significant interest to examine the structure of this conotoxin...
  11. doi request reprint Engineering stabilized vascular endothelial growth factor-A antagonists: synthesis, structural characterization, and bioactivity of grafted analogues of cyclotides
    Sunithi Gunasekera
    The University of Queensland, Institute for Molecular Bioscience, Brisbane QLD 4072, Australia
    J Med Chem 51:7697-704. 2008
    ..In general, the stabilization of bioactive peptide epitopes is a significant problem in medicinal chemistry and in the current study we have provided insight into one approach to stabilize these peptides in a biological environment...
  12. ncbi request reprint Alpha-selenoconotoxins, a new class of potent alpha7 neuronal nicotinic receptor antagonists
    Christopher J Armishaw
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 281:14136-43. 2006
    ..These results demonstrate that selenoconotoxins can be used as highly stable scaffolds for the design of new drugs...
  13. doi request reprint Effects of cyclization on stability, structure, and activity of α-conotoxin RgIA at the α9α10 nicotinic acetylcholine receptor and GABA(B) receptor
    Reena Halai
    Institute for Molecular Bioscience, Division of Chemistry and Structural Biology, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Med Chem 54:6984-92. 2011
    ....
  14. ncbi request reprint The cyclotide family of circular miniproteins: nature's combinatorial peptide template
    David J Craik
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane
    Biopolymers 84:250-66. 2006
    ..Cyclotides are exceptionally stable and are resistant to denaturation via thermal, chemical, or enzymatic treatments. The structural features that contribute to their remarkable stability are described in this review...
  15. doi request reprint Solving the alpha-conotoxin folding problem: efficient selenium-directed on-resin generation of more potent and stable nicotinic acetylcholine receptor antagonists
    Markus Muttenthaler
    Institute for Molecular Bioscience, Division of Chemistry and Structural Biology, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Am Chem Soc 132:3514-22. 2010
    ....
  16. doi request reprint Isolation and characterization of peptides from Momordica cochinchinensis seeds
    Lai Y Chan
    The University of Queensland, Institute for Molecular Bioscience, Brisbane QLD 4072, Australia
    J Nat Prod 72:1453-8. 2009
    ..Of the cell lines tested, MCoCC-1 is the most toxic against a human melanoma cell line (MM96L) and is nonhemolytic to human erythrocytes. The role of these peptides within the plant remains to be determined...
  17. ncbi request reprint Solution structure of chi-conopeptide MrIA, a modulator of the human norepinephrine transporter
    K Peter R Nilsson
    Institute for Molecular Bioscience, University of Queensland, Brisbane 4072, Australia
    Biopolymers 80:815-23. 2005
    ..The N-terminus also affects activity, as a single N-terminal deletion introduced additional pharmacology at rat vas deferens, while deleting the first two amino acids reduced chi-conopeptide potency...
  18. ncbi request reprint The cyclotide fingerprint in oldenlandia affinis: elucidation of chemically modified, linear and novel macrocyclic peptides
    Manuel Rey R Plan
    Institute for Molecular Bioscience, Australian Research Council, Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane QLD 4072, Australia
    Chembiochem 8:1001-11. 2007
    ..These pathways have important implications for the persistence and environmental fate of the cyclotides if used as crop-protection agents...
  19. doi request reprint Alanine scanning mutagenesis of the prototypic cyclotide reveals a cluster of residues essential for bioactivity
    Shane M Simonsen
    Institute for Molecular Bioscience, University of Queensland, St Lucia, Brisbane, Queensland 4072, Australia
    J Biol Chem 283:9805-13. 2008
    ..Overall, the mutagenesis data provide an important insight into cyclotide biological activity and suggest that specific self-association, in combination with membrane binding mediates cyclotide bioactivities...
  20. ncbi request reprint Potential therapeutic applications of the cyclotides and related cystine knot mini-proteins
    David J Craik
    University of Queensland, Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, Brisbane QLD 4072, Australia
    Expert Opin Investig Drugs 16:595-604. 2007
    ..The cystine knot framework is tolerant to a wide range of residue substitutions and is showing great promise as a scaffold in drug design and protein engineering...
  21. pmc Engineering stable peptide toxins by means of backbone cyclization: stabilization of the alpha-conotoxin MII
    Richard J Clark
    Institute for Molecular Bioscience and School of Biomedical Sciences, University of Queensland, Brisbane QLD 4072, Australia
    Proc Natl Acad Sci U S A 102:13767-72. 2005
    ..Cyclization strategies represent an approach for stabilizing bioactive peptides while keeping their full potencies and should boost applications of peptide-based drugs in human medicine...
  22. ncbi request reprint Conserved structural and sequence elements implicated in the processing of gene-encoded circular proteins
    Julie L Dutton
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Quuensland 4072, Australia
    J Biol Chem 279:46858-67. 2004
    ..This structural conservation suggests a vital role for the NTR in the in vivo folding, processing, or detoxification of cyclotide domains from the precursor protein...
  23. pmc Cyclic peptides arising by evolutionary parallelism via asparaginyl-endopeptidase-mediated biosynthesis
    Joshua S Mylne
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Plant Cell 24:2765-78. 2012
    ..We believe this is evolutionary evidence that, in addition to its known roles in proteolysis, AEP is especially suited to performing protein cyclization...
  24. doi request reprint A Synthetic mirror image of kalata B1 reveals that cyclotide activity is independent of a protein receptor
    Lillian Sando
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
    Chembiochem 12:2456-62. 2011
    ....
  25. ncbi request reprint Retrocyclin-2: structural analysis of a potent anti-HIV theta-defensin
    Norelle L Daly
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane QLD 4072, Australia
    Biochemistry 46:9920-8. 2007
    ..The ability to self-associate may contribute to the high-affinity binding of retrocyclins for glycoproteins by increasing the valency and enhancing the ability of retrocyclins to cross-link cell surface glycoproteins...
  26. ncbi request reprint The absolute structural requirement for a proline in the P3'-position of Bowman-Birk protease inhibitors is surmounted in the minimized SFTI-1 scaffold
    Norelle L Daly
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 281:23668-75. 2006
    ..Overall, this mutational analysis of SFTI-1 clearly defines the optimized nature of the SFTI-1 scaffold and demonstrates the importance of the secondary loop in maintaining the active conformation of the binding loop...
  27. doi request reprint The structure of a two-disulfide intermediate assists in elucidating the oxidative folding pathway of a cyclic cystine knot protein
    Masa Cemazar
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Structure 16:842-51. 2008
    ....
  28. doi request reprint Structural and biochemical characteristics of the cyclotide kalata B5 from Oldenlandia affinis
    Manuel R Plan
    Institute for Molecular Bioscience, Division of Chemistry and Structural Biology, The University of Queensland, 306 Carmody Rd, St Lucia, QLD 4067, Australia
    Biopolymers 94:647-58. 2010
    ..As is the case with other cyclotides, kalata B5 has an exposed hydrophobic region on its surface, supporting suggestions that this hydrophobic patch is a key feature for membrane binding and biological activity of cyclotides...
  29. doi request reprint Stabilization of α-conotoxin AuIB: influences of disulfide connectivity and backbone cyclization
    Erica S Lovelace
    Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Australia
    Antioxid Redox Signal 14:87-95. 2011
    ..Overall, the results of this study provide important insights into factors influencing the stability and oxidative folding of α-conotoxin AuIB and might be valuable in the design of more stable antagonists of nAChRs...
  30. ncbi request reprint Cyclic MrIA: a stable and potent cyclic conotoxin with a novel topological fold that targets the norepinephrine transporter
    Erica S Lovelace
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Med Chem 49:6561-8. 2006
    ..Furthermore, the structure reported here for cyclic MrIA represents a new topology among a growing number of circular disulfide-rich peptides...
  31. pmc Isolation of an orally active insecticidal toxin from the venom of an Australian tarantula
    Margaret C Hardy
    Institute for Molecular Bioscience, University of Queensland, St Lucia, Australia
    PLoS ONE 8:e73136. 2013
    ..OAIP-1 is likely to be synergized by the gut-lytic activity of the Bacillus thuringiensis Cry toxin (Bt) expressed in insect-resistant transgenic crops, and consequently it might be a good candidate for trait stacking with Bt. ..
  32. doi request reprint Isolation and characterization of α-conotoxin LsIA with potent activity at nicotinic acetylcholine receptors
    Marco C Inserra
    Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane QLD 4072, Australia
    Biochem Pharmacol 86:791-9. 2013
    ..This study gives further insight into α-conotoxin pharmacology and the molecular basis of nAChR selectivity, highlighting the influence of N-terminal residues and C-terminal amidation on conotoxin pharmacology...
  33. doi request reprint Engineering pro-angiogenic peptides using stable, disulfide-rich cyclic scaffolds
    Lai Y Chan
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Blood 118:6709-17. 2011
    ..This is the first report of using these scaffolds to improve the activity and stability of angiogenic peptide sequences and is a promising approach for promoting angiogenesis for therapeutic uses...
  34. ncbi request reprint Chemical synthesis and biosynthesis of the cyclotide family of circular proteins
    Sunithi Gunasekera
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane Queensland, Australia
    IUBMB Life 58:515-24. 2006
    ..In this review the current chemical, recombinant and biosynthetic routes to the production of cyclotides are discussed...
  35. ncbi request reprint Capped acyclic permutants of the circular protein kalata B1
    Shane M Simonsen
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    FEBS Lett 577:399-402. 2004
    ..These variants were observed to cause no measurable lysis of erythrocytes, strengthening the connection between backbone cyclization and haemolytic activity...
  36. pmc Kalata B8, a novel antiviral circular protein, exhibits conformational flexibility in the cystine knot motif
    Norelle L Daly
    Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, QLD 4072, Australia
    Biochem J 393:619-26. 2006
    ..The 'uncoupling' of these two activities has not previously been observed for the cyclotides and may be related to the unusual hydrophilic nature of the peptide...
  37. doi request reprint Cyclization of the antimicrobial peptide gomesin with native chemical ligation: influences on stability and bioactivity
    Lai Yue Chan
    The University of Queensland, Institute for Molecular Bioscience, 306 Carmody Road, Brisbane 4072, Australia
    Chembiochem 14:617-24. 2013
    ..In addition, antimalarial activity is enhanced upon cyclization. These findings provide additional insight into the influences of backbone cyclization on the therapeutic potential of peptides...
  38. ncbi request reprint Engineering of conotoxins for the treatment of pain
    Bodil B Carstens
    The University of Queensland, Institute for Molecular Bioscience, Brisbane QLD 4072, Australia
    Curr Pharm Des 17:4242-53. 2011
    ..Minor re-engineering of χ-conotoxin MrIa to convert its N-terminal residue to pyroglutamic acid proved particularly successful and the modified derivative, Xen2174, is currently in clinical trials for neuropathic pain...
  39. ncbi request reprint Discovery and characterization of a linear cyclotide from Viola odorata: implications for the processing of circular proteins
    David C Ireland
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, QLD 4072, Australia
    J Mol Biol 357:1522-35. 2006
    ..Enzymatic stability assays on violacin A indicate that despite an increase in the flexibility of the structure relative to cyclic counterparts, the cystine knot preserves the overall stability of the molecule...
  40. doi request reprint Dissecting the oxidative folding of circular cystine knot miniproteins
    Sunithi Gunasekera
    The University of Queensland, Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, Brisbane, Australia
    Antioxid Redox Signal 11:971-80. 2009
    ..Overall, these mechanistic findings on the folding of cyclotides are potentially valuable for the design of new drug leads...
  41. ncbi request reprint Solution structure of the cyclotide palicourein: implications for the development of a pharmaceutical framework
    Daniel G Barry
    Institute for Molecular Bioscience, Queensland Bioscience Precinct, The University of Queensland Brisbane, Queensland 4072, Australia
    Structure 12:85-94. 2004
    ..The cyclotide core fold, thus, can in principle be used as a framework for the development of useful pharmaceutical and agricultural bioactivities...
  42. ncbi request reprint The cyclotides and related macrocyclic peptides as scaffolds in drug design
    David J Craik
    University of Queensland, Institute for Molecular Bioscience, Australian Research Council Special Research Center for Functional and Applied Genomics, Brisbane
    Curr Opin Drug Discov Devel 9:251-60. 2006
    ....
  43. doi request reprint Discovery of cyclotides in the fabaceae plant family provides new insights into the cyclization, evolution, and distribution of circular proteins
    Aaron G Poth
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD, Australia
    ACS Chem Biol 6:345-55. 2011
    ..Moreover, this study provides impetus for the examination of other economically and agriculturally significant species within Fabaceae, now the largest plant family from which cyclotides have been described...
  44. doi request reprint A new family of cystine knot peptides from the seeds of Momordica cochinchinensis
    Lai Yue Chan
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Peptides 39:29-35. 2013
    ..Otherwise these new peptides MCo-3 to MCo-6 were evaluated for antimalarial activity against Plasmodium falciparum, and cytotoxic activity against the cancer cell line MDA-MB-231. But these peptides were not active...
  45. doi request reprint Cyclization of conotoxins to improve their biopharmaceutical properties
    Richard J Clark
    The University of Queensland, Institute for Molecular Bioscience, Brisbane, QLD 4072, Australia
    Toxicon 59:446-55. 2012
    ....
  46. doi request reprint Discovery, structure and biological activities of cyclotides
    Norelle L Daly
    The University of Queensland, Institute for Molecular Bioscience, Brisbane, Australia
    Adv Drug Deliv Rev 61:918-30. 2009
    ..This article gives an overview of the discovery of cyclotides, describes their unique structural features and range of bioactivities, and discusses their applications in drug design...
  47. pmc Discovery of an unusual biosynthetic origin for circular proteins in legumes
    Aaron G Poth
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    Proc Natl Acad Sci U S A 108:10127-32. 2011
    ..Knowledge of Fabaceae cyclotide gene transcripts should enable the production of modified cyclotides in crop plants for a variety of agricultural or pharmaceutical applications, including plant-produced designer peptide drugs...
  48. doi request reprint The three-dimensional structure of the analgesic alpha-conotoxin, RgIA
    Richard J Clark
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, Australia
    FEBS Lett 582:597-602. 2008
    ..We determined the three-dimensional structure of RgIA using NMR methods to assist in elucidating the molecular role of RgIA in analgesia and inflammation...
  49. ncbi request reprint Oxidative folding of the cystine knot motif in cyclotide proteins
    David J Craik
    Institute for Molecular Bioscience, University of Queensland, Brisbane, 4072, Australia
    Protein Pept Lett 12:147-52. 2005
    ..This mini-review reports on the current understanding of the folding process in cyclotides. The synthesis and folding of these molecules paves the way for their application as stable molecular templates...
  50. ncbi request reprint Disulfide folding pathways of cystine knot proteins. Tying the knot within the circular backbone of the cyclotides
    Norelle L Daly
    Institute for Molecular Bioscience, Australian Research Council Centre for Functional and Applied Genomics, University of Queensland, Brisbane, 4072 Queensland, Australia
    J Biol Chem 278:6314-22. 2003
    ....
  51. pmc Structural plasticity of the cyclic-cystine-knot framework: implications for biological activity and drug design
    Richard J Clark
    Institute for Molecular Bioscience, Australian Research Council Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, QLD 4072, Australia
    Biochem J 394:85-93. 2006
    ..This finding confirms the tolerance of the cyclotide framework to residue substitutions and opens up possibilities for the substitution of biologically active peptide epitopes into the framework...
  52. ncbi request reprint NMR as a tool for elucidating the structures of circular and knotted proteins
    David J Craik
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane QLD 4072, Australia
    Mol Biosyst 3:257-65. 2007
    ..In this review the role of NMR in defining the structures of cyclotides is described...
  53. pmc Chemical re-engineering of chlorotoxin improves bioconjugation properties for tumor imaging and targeted therapy
    Muharrem Akcan
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    J Med Chem 54:782-7. 2011
    ..Based on these data, we propose to advance a monolabeled chlorotoxin to human clinical trials...
  54. doi request reprint α-Conotoxin ImI incorporating stable cystathionine bridges maintains full potency and identical three-dimensional structure
    Zoltan Dekan
    Institute for Molecular Bioscience, The University of Queensland, St Lucia 4072, Queensland, Australia
    J Am Chem Soc 133:15866-9. 2011
    ..The effect of oxidation of the thioethers to sulfoxides was also investigated, with significant changes in the biological activities observed ranging from a >30-fold reduction (2S═O) to a 3-fold increase (3S═O(B)) in potencies...
  55. ncbi request reprint Isolation, structure, and activity of GID, a novel alpha 4/7-conotoxin with an extended N-terminal sequence
    Annette Nicke
    Institute for Molecular Bioscience and School of Biomedical Sciences, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 278:3137-44. 2003
    ....
  56. doi request reprint NMR and protein structure in drug design: application to cyclotides and conotoxins
    Norelle L Daly
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    Eur Biophys J 40:359-70. 2011
    ..Chemically re-engineering conotoxins to give them a cyclic backbone has been used to engender them with improved biopharmaceutical properties, such as are observed in cyclotides...
  57. ncbi request reprint A comparison of the self-association behavior of the plant cyclotides kalata B1 and kalata B2 via analytical ultracentrifugation
    Amanda Nourse
    Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, QLD 4072, Australia
    J Biol Chem 279:562-70. 2004
    ..An understanding of the factors affecting solution aggregation is of vital importance for future pharmaceutical application of these molecules...
  58. pmc Structures of naturally occurring circular proteins from bacteria
    David J Craik
    Institute for Molecular Bioscience, The University of Queensland, Brisbane QLD 4072, Australia
    J Bacteriol 185:4011-21. 2003
  59. ncbi request reprint Solution structure and novel insights into the determinants of the receptor specificity of human relaxin-3
    K Johan Rosengren
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 281:5845-51. 2006
    ..This structural feature is likely important for the activation of its endogenous receptor, GPCR135...
  60. doi request reprint α-Conotoxin MrIC is a biased agonist at α7 nicotinic acetylcholine receptors
    Alexander Mueller
    Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, St Lucia, Queensland 4072, Australia
    Biochem Pharmacol 94:155-63. 2015
    ....
  61. pmc The cyclic cystine ladder in θ-defensins is important for structure and stability, but not antibacterial activity
    Anne C Conibear
    Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 288:10830-40. 2013
    ..The results provide insights into the mechanism of action of θ-defensins and illustrate the potential of θ-defensin analogues as scaffolds for peptide drug design...
  62. ncbi request reprint Chemical synthesis and structure elucidation of bovine kappa-casein (1-44)
    Paramjit S Bansal
    Institute for Molecular Bioscience, The University of Queensland, St Lucia, QLD 4072, Australia
    Biochem Biophys Res Commun 340:1098-103. 2006
    ..Further, NMR analysis showed the presence of a helical segment containing 26 residues which extends from Pro8 to Arg34. This is the first report which demonstrates extensive secondary structure within the casein class of proteins...
  63. doi request reprint Isolation, sequencing, and structure-activity relationships of cyclotides
    David C Ireland
    Division of Chemistry and Structural Biology, Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
    J Nat Prod 73:1610-22. 2010
    ..They have thus attracted interest in drug design as well as in crop protection applications...
  64. ncbi request reprint Linearization of a naturally occurring circular protein maintains structure but eliminates hemolytic activity
    Daniel G Barry
    Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane 4072, Australia
    Biochemistry 42:6688-95. 2003
    ..Furthermore, loss of hemolytic activity is associated with backbone truncation and linearization...
  65. doi request reprint Chemical synthesis and structure of the prokineticin Bv8
    Rodrigo A V Morales
    Institute for Molecular Bioscience, The University of Queensland, 306 Carmody Road, St Lucia, Brisbane, Australia
    Chembiochem 11:1882-8. 2010
    ....
  66. pmc Identification and characterization of a new family of cell-penetrating peptides: cyclic cell-penetrating peptides
    Laura Cascales
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 286:36932-43. 2011
    ..The ability of diverse disulfide-rich cyclic peptides to penetrate cells enhances their potential in drug design, and we propose a new classification for them, i.e. cyclic cell-penetrating peptides...
  67. ncbi request reprint Microcin J25 has a threaded sidechain-to-backbone ring structure and not a head-to-tail cyclized backbone
    K Johan Rosengren
    Institute for Molecular Bioscience, University of Queensland, Brisbane QLD 4072, Australia
    J Am Chem Soc 125:12464-74. 2003
    ..The new structural interpretation fully accounts for previously reported NMR and biophysical data and is consistent with the remarkable stability of this potent antimicrobial peptide...
  68. pmc The biological activity of the prototypic cyclotide kalata b1 is modulated by the formation of multimeric pores
    Yen Hua Huang
    Institute for Molecular Bioscience, The University of Queensland, Brisbane 4072, Australia
    J Biol Chem 284:20699-707. 2009
    ..Collectively, the findings provide a mechanistic explanation for the diversity of biological functions ascribed to this fascinating family of ultrastable macrocyclic peptides...
  69. doi request reprint Albumins and their processing machinery are hijacked for cyclic peptides in sunflower
    Joshua S Mylne
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia
    Nat Chem Biol 7:257-9. 2011
    ..Thus, these peptides emerge from within an albumin precursor by the action of albumin's own processing enzyme...
  70. doi request reprint Beta-arrestin 2 is required for complement C1q expression in macrophages and constrains factor-independent survival
    Jane E Lattin
    The University of Queensland, Institute for Molecular Bioscience, QLD 4072, Australia
    Mol Immunol 47:340-7. 2009
    ..Given that loss of C1q function is strongly associated with the development of systemic lupus erythematosus, ARRB2 may act to limit the development of autoimmune disease...
  71. ncbi request reprint Structures of muO-conotoxins from Conus marmoreus. I nhibitors of tetrodotoxin (TTX)-sensitive and TTX-resistant sodium channels in mammalian sensory neurons
    Norelle L Daly
    Institute for Molecular Bioscience and School of Biomedical Sciences, University of Queensland, Brisbane, 4072 Queensland, Australia
    J Biol Chem 279:25774-82. 2004
    ....
  72. ncbi request reprint Structure-activity relationships of alpha-conotoxins targeting neuronal nicotinic acetylcholine receptors
    Emma L Millard
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia
    Eur J Biochem 271:2320-6. 2004
    ..Structural studies, particularly using NMR spectroscopy have provided an insight into the role and spatial location of residues implicated in receptor binding and biological activity...
  73. pmc Decoding the membrane activity of the cyclotide kalata B1: the importance of phosphatidylethanolamine phospholipids and lipid organization on hemolytic and anti-HIV activities
    Sónia Troeira Henriques
    University of Queensland, Institute for Molecular Bioscience, Brisbane, Queensland 4072, Australia
    J Biol Chem 286:24231-41. 2011
    ..We further show that the anti-HIV activity of kB1 is the result of its ability to target and disrupt the membranes of HIV particles, which are raft-like membranes very rich in PE-phospholipids...
  74. ncbi request reprint Discovery, structure and biological activities of the cyclotides
    David J Craik
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    Curr Protein Pept Sci 5:297-315. 2004
    ..Because of their highly stable peptide framework they might make useful templates in drug design programs, and their insecticidal activity opens the possibility of applications in crop protection...
  75. ncbi request reprint Structure and metal binding studies of the second copper binding domain of the Menkes ATPase
    Christopher E Jones
    The National Research Centre for Environmental Toxicology, The University of Queensland, 39 Kessels Road, Coopers Plains, QLD 4108, Australia
    J Struct Biol 143:209-18. 2003
    ..Complementary site-directed mutagenesis of the adjacent residues has been used to probe the structural roles of conserved residues...
  76. ncbi request reprint Twists, knots, and rings in proteins. Structural definition of the cyclotide framework
    K Johan Rosengren
    Institute for Molecular Bioscience, Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, Australia
    J Biol Chem 278:8606-16. 2003
    ....
  77. ncbi request reprint The cyclotides: novel macrocyclic peptides as scaffolds in drug design
    David J Craik
    Institute for Molecular Bioscience, Australian Research Council Special Research Center for Functional and Applied Genomics, University of Queensland, Brisbane, QLD 4072, Australia
    Curr Opin Drug Discov Devel 5:251-60. 2002
    ..The potential use of cyclotides for drug design is evaluated here, with reference to rapidly increasing knowledge of natural cyclotides and the emergence of new techniques in peptide engineering...
  78. doi request reprint Cyclotides: macrocyclic peptides with applications in drug design and agriculture
    David J Craik
    Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, 4072, Australia
    Cell Mol Life Sci 67:9-16. 2010
    ..This article identifies gaps in current knowledge on cyclotides and suggests future directions for research into this fascinating family of ultra-stable mini-proteins...
  79. ncbi request reprint The chemistry and biology of cyclotides
    David J Craik
    University of Queensland, Institute for Molecular Bioscience, Australian Research Council Special Research Center for Functional and Applied Genomics, Brisbane, QLD 4072, Australia
    Curr Opin Drug Discov Devel 10:176-84. 2007
    ..The availability of an enhanced armory of synthetic methods promises to expand the potential range of cyclotide-based applications...
  80. ncbi request reprint Solution structures of the cis- and trans-Pro30 isomers of a novel 38-residue toxin from the venom of Hadronyche Infensa sp. that contains a cystine-knot motif within its four disulfide bonds
    K Johan Rosengren
    Institute for Molecular Bioscience, ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane QLD 4072, Australia
    Biochemistry 41:3294-301. 2002
    ..Full assignment of the NMR spectra for both conformers allowed for the calculation of their structures, revealing the presence of a triple-stranded antiparallel beta sheet consistent with the inhibitor cystine-knot (ICK) motif...
  81. ncbi request reprint The role of the cyclic peptide backbone in the anti-HIV activity of the cyclotide kalata B1
    Norelle L Daly
    Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD 4072, Australia
    FEBS Lett 574:69-72. 2004
    ..In addition, acyclic permutants of kalata B1 displayed no anti-HIV activity, suggesting that this activity is critically dependent on an intact circular backbone...
  82. ncbi request reprint NMR of conotoxins: structural features and an analysis of chemical shifts of post-translationally modified amino acids
    Ute C Marx
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane QLD 4072, Australia
    Magn Reson Chem 44:S41-50. 2006
    ..This analysis provides a reference source for chemical shifts of post-translationally modified amino acids...
  83. doi request reprint Conopressin-T from Conus tulipa reveals an antagonist switch in vasopressin-like peptides
    Sebastien Dutertre
    Institute for Molecular Bioscience, University of Queensland, Brisbane, Queensland 4072, Australia
    J Biol Chem 283:7100-8. 2008
    ..NMR structures of both conopressin-T and L7P analogue revealed a marked difference in the orientation of the exocyclic tripeptide that may serve as templates for the design of novel ligands with enhanced affinity for the V 1a receptor...
  84. ncbi request reprint The cyclic cystine knot miniprotein MCoTI-II is internalized into cells by macropinocytosis
    Kathryn P Greenwood
    Institute for Molecular Bioscience and Australian Research Council Special Research Centre for Functional and Applied Genomics, The University of Queensland, Brisbane, Queensland 4072, Australia
    Int J Biochem Cell Biol 39:2252-64. 2007
    ..The ready uptake, coupled with low cytotoxicity, indicates that MCoTI-II has the potential to transport grafted bioactivities to intracellular targets, making it a potentially valuable framework in drug design applications...