Stuart I Mannering
Affiliation: The Walter and Eliza Hall Institute of Medical Research
- Interleukin-2 and loss of immunity in experimental Mycobacterium avium infectionStuart I Mannering
Department of Microbiology and Immunology, University of Melbourne, Victoria 3010, Australia
Infect Immun 70:27-35. 2002..However, CD25 (IL-2Ralpha) continued to be highly expressed in the terminal stages of infection, and although IL-2 production declined, addition of recombinant IL-2 to cultures could not rescue the final loss of IFN-gamma production...
- T cell epitopes of the La/SSB autoantigen in humanized transgenic mice expressing the HLA class II haplotype DRB1*0301/DQB1*0201Nadine L Dudek
Bio21 Molecular Science and Biotechnology Institute, and University of Melbourne, Melbourne, Victoria, Australia
Arthritis Rheum 56:3387-98. 2007..This study was undertaken to investigate the DR3/DQ2-restricted T cell response to wild-type human La (hLa) and a truncated form of mutant La...
- Recent insights into CD4(+) T-cell specificity and function in Type 1 diabetesStuart I Mannering
JDRF Advanced Postdoctoral Fellow, Autoimmunity and Transplantation Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
Expert Rev Clin Immunol 3:557-64. 2007..Here we discuss, with particular reference to human studies, advances in our understanding of CD4(+) T-cell responses in T1D...
- The insulin A-chain epitope recognized by human T cells is posttranslationally modifiedStuart I Mannering
Autoimmunity and Transplantation Division, The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Parkville, Victoria, Australia
J Exp Med 202:1191-7. 2005..We define for the first time a novel posttranslational modification that is required for T cell recognition of the insulin A-chain in T1D...
- An efficient method for cloning human autoantigen-specific T cellsStuart I Mannering
Autoimmunity and Transplantation Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
J Immunol Methods 298:83-92. 2005..Sixty proinsulin- and 47 glutamic acid decarboxylase-specific clones were obtained from six and two donors, respectively. In conclusion, the CFSE-based method is ideal for cloning rare, autoantigen-specific, human CD4+ T cells...
- CD4+ T cell proliferation in response to GAD and proinsulin in healthy, pre-diabetic, and diabetic donorsStuart I Mannering
Virology, Victorian Infectious Diseases Reference Lab, 10 Wreckyn, North Melbourne, Victoria, 3051, Australia
Ann N Y Acad Sci 1037:16-21. 2004..In addition, the ability of the CFSE assay to allow analysis of the phenotype and function of autoantigen-responsive T cells may improve discrimination...
- A sensitive method for detecting proliferation of rare autoantigen-specific human T cellsStuart I Mannering
Autoimmunity and Transplantation Division, The Walter and Eliza Hall Institute, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia
J Immunol Methods 283:173-83. 2003..Using the CFSE assay we were able to measure directly the proliferation of human CD4(+) T cells from healthy donors in response to the type 1 diabetes autoantigens glutamic acid decarboxylase (GAD) and proinsulin (PI)...
- Human T-cells recognise N-terminally Fmoc-modified peptideStuart I Mannering
Autoimmunity and Transplantation Division, The Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, 1G Royal Parade, Parkville, VIC 3050, Australia
Vaccine 21:3638-46. 2003..This finding has important implications for the use of synthetic peptides in screening and epitope mapping studies and their use as vaccines in humans...
- Generation and expansion of regulatory human CD4(+) T-cell clones specific for pancreatic islet autoantigensJames A Dromey
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, 3050 Victoria, Australia
J Autoimmun 36:47-55. 2011..This study demonstrates that autoantigen-specific CD4(+) Treg clones with potential application as a cell therapy for autoimmune disease can be generated and expanded from human blood...
- Intranasal vaccination with proinsulin DNA induces regulatory CD4+ T cells that prevent experimental autoimmune diabetesAlison L Every
Autoimmunity and Transplantation Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
J Immunol 176:4608-15. 2006....
- T-cell activation, proliferation and apoptosis in primary Listeria monocytogenes infectionStuart I Mannering
Department of Microbiology and Immunology, University of Melbourne, Victoria Australia
Immunology 106:87-95. 2002..Hence, CD25 expression peaked early, but expression of other activation markers, in vivo proliferation and apoptosis coincided after Listeria infection. T cells that had proliferated were over-represented in the apoptotic population...
- Monoclonal antibodies generated by DNA immunization recognize CD2 from a broad range of primatesJamie L Brady
Division of Autoimmunity and Transplantation, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
Immunol Cell Biol 87:413-8. 2009..Using chimeric NOD-SCID IL2rgamma(null) mice, the mAb were shown to deplete human and cynomolgus monkey T cells in vivo. These anti-CD2 mAb may therefore be important immunological tools in allo- and xenotransplantation...
- Pathogenic mechanisms in type 1 diabetes: the islet is both target and driver of diseaseKate L Graham
St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
Rev Diabet Stud 9:148-68. 2012..New technology, especially MHC tetramers and modern imaging, will enhance our understanding of the pathogenic mechanisms...
- Residual methylprednisolone suppresses human T-cell responses to spleen, but not islet, extracts from deceased organ donorsMax Joffe
Immunology and Diabetes Unit, St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
Int Immunol 24:447-53. 2012..We conclude that extracts of human spleen, but not islets, from deceased organ donors contain sufficient residual methylprednisolone to suppress the proliferation of T-cells in vitro...
- Mouse pancreatic beta cells express MHC class II and stimulate CD4(+) T cells to proliferateYuxing Zhao
St Vincent s Institute, Immunology and Diabetes Laboratory, Fitzroy, Victoria, Australia
Eur J Immunol 45:2494-503. 2015..Our study indicates that MHC class II molecules may play an important role in beta-cell interaction with CD4(+) T cells in the development of type 1 diabetes. ..
- Human dendritic cell subsets from spleen and blood are similar in phenotype and function but modified by donor health statusDiana Mittag
Autoimmunity and Transplantation Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
J Immunol 186:6207-17. 2011..In particular, the hallmark functions of mouse CD8α(+) DC subsets, that is, IL-12p70 secretion and cross-presentation, are not confined to the equivalent human CD141(+) DC but are shared by CD1b/c(+) and CD16(+) DC subsets...
- Proinsulin-a pathogenic autoantigen in type 1 diabetesParth Narendran
The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, 3050 Parkville, Australia
Autoimmun Rev 2:204-10. 2003..We conclude that proinsulin satisfies these criteria. As a corollary, proinsulin is a potential immunotherapeutic tool for the prevention of T1D...
- TCR-mediated activation promotes GITR upregulation in T cells and resistance to glucocorticoid-induced deathYifan Zhan
Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Melbourne 3050, Australia
Int Immunol 16:1315-21. 2004..Our findings also have implications for GC treatment in that it would be more difficult to abrogate an ongoing T-cell mediated inflammatory response than to prevent its induction...
- Proinsulin-specific, HLA-DQ8, and HLA-DQ8-transdimer-restricted CD4+ T cells infiltrate islets in type 1 diabetesVimukthi Pathiraja
Immunology and Diabetes Unit, St Vincent s Institute of Medical Research, Fitzroy, Victoria, Australia
Diabetes 64:172-82. 2015..Hence, proinsulin-specific, HLA-DQ8, and HLA-DQ8-transdimer-restricted CD4(+) T cells are strongly implicated in the autoimmune pathogenesis of human T1D...
- Biased T cell receptor usage directed against human leukocyte antigen DQ8-restricted gliadin peptides is associated with celiac diseaseSophie E Broughton
The Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria, Australia
Immunity 37:611-21. 2012..Accordingly, biased TCR usage is an important factor in the pathogenesis of DQ8-mediated celiac disease...
- Comprehensive, quantitative mapping of T cell epitopes in gluten in celiac diseaseJason A Tye-Din
Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
Sci Transl Med 2:41ra51. 2010..Our findings show that pathogenic T cells in celiac disease show limited diversity, and therefore suggest that peptide-based therapeutics for this disease and potentially other strongly HLA-restricted immune diseases should be possible...
- Congenic mice reveal genetic epistasis and overlapping disease loci for autoimmune diabetes and listeriosisNancy Wang
Immunology and Diabetes Unit, St Vincent s Institute of Medical Research, 9 Princes Street, Fitzroy, Victoria, 3065, Australia
Immunogenetics 66:501-6. 2014..This result co-localizes Idd14 and Listr2, a resistance locus for listeriosis, to the same genomic interval and indicates that congenic NOD mice may also be useful for localizing resistance loci for infectious disease. ..
- Cytotoxic T-lymphocyte-mediated killing of human pancreatic islet cells in vitroPeter D Campbell
St Vincent s Institute, The University of Melbourne Department of Medicine, St Vincent s Hospital, Fitzroy, Australia
Hum Immunol 69:543-51. 2008..In conclusion, we report a novel way of investigating the interaction between CTL and human islets. Human islets were rapidly killed in vitro by MHC class I-restricted CTL predominantly by the granule exocytosis pathway...
- A structural and immunological basis for the role of human leukocyte antigen DQ8 in celiac diseaseKate N Henderson
The Protein Crystallography Unit, Department of Biochemistry and Molecular Biology, School of Biomedical Sciences, Monash University, Clayton, Victoria 3800, Australia
Immunity 27:23-34. 2007..Accordingly, nondietary therapeutic interventions in celiac disease might need to be tailored to the genotype of the individual...