Brendan S Crabb

Summary

Affiliation: The Walter and Eliza Hall Institute of Medical Research
Country: Australia

Publications

  1. pmc A new rodent model to assess blood stage immunity to the Plasmodium falciparum antigen merozoite surface protein 119 reveals a protective role for invasion inhibitory antibodies
    Tania F de Koning-Ward
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville Victoria 3050, Australia
    J Exp Med 198:869-75. 2003
  2. pmc Antibodies against merozoite surface protein (MSP)-1(19) are a major component of the invasion-inhibitory response in individuals immune to malaria
    T F de Koning-Ward
    Department of Microbiology and Immunology and the Co-operative Research Centre for Vaccine Technology, University of Melbourne, VIC 3010, Australia
    J Exp Med 193:1403-12. 2001
  3. ncbi request reprint Transfection technology and the study of drug resistance in the malaria parasite Plasmodium falciparum
    Brendan S Crabb
    The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Vic, Australia
    Drug Resist Updat 5:126-30. 2002
  4. pmc Plasmodium falciparum virulence determinants unveiled
    Brendan S Crabb
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia
    Genome Biol 3:REVIEWS1031. 2002
  5. ncbi request reprint A new system for rapid plasmid integration in Plasmodium parasites
    Brendan S Crabb
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne 3050, Australia
    Trends Microbiol 15:3-6. 2007
  6. pmc Promising functional readouts of immunity in a blood-stage malaria vaccine trial
    Brendan S Crabb
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia
    PLoS Med 2:e380. 2005
  7. ncbi request reprint A common cross-species function for the double epidermal growth factor-like modules of the highly divergent plasmodium surface proteins MSP-1 and MSP-8
    Damien R Drew
    Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria 3050, Australia
    J Biol Chem 279:20147-53. 2004
  8. pmc Evidence for a common role for the serine-type Plasmodium falciparum serine repeat antigen proteases: implications for vaccine and drug design
    Joanne E McCoubrie
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    Infect Immun 75:5565-74. 2007
  9. ncbi request reprint A var gene promoter controls allelic exclusion of virulence genes in Plasmodium falciparum malaria
    Till S Voss
    The Walter and Eliza Hall Institute of Medical Research, Parkville 3050, Australia
    Nature 439:1004-8. 2006
  10. doi request reprint Blood-stage Plasmodium berghei infection leads to short-lived parasite-associated antigen presentation by dendritic cells
    Rachel J Lundie
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    Eur J Immunol 40:1674-81. 2010

Detail Information

Publications90

  1. pmc A new rodent model to assess blood stage immunity to the Plasmodium falciparum antigen merozoite surface protein 119 reveals a protective role for invasion inhibitory antibodies
    Tania F de Koning-Ward
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville Victoria 3050, Australia
    J Exp Med 198:869-75. 2003
    ..This study provides a strong impetus for the development of blood stage vaccines designed to generate invasion inhibitory antibodies and offers a new animal model to trial P. falciparum MSP-119 vaccines...
  2. pmc Antibodies against merozoite surface protein (MSP)-1(19) are a major component of the invasion-inhibitory response in individuals immune to malaria
    T F de Koning-Ward
    Department of Microbiology and Immunology and the Co-operative Research Centre for Vaccine Technology, University of Melbourne, VIC 3010, Australia
    J Exp Med 193:1403-12. 2001
    ....
  3. ncbi request reprint Transfection technology and the study of drug resistance in the malaria parasite Plasmodium falciparum
    Brendan S Crabb
    The Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Vic, Australia
    Drug Resist Updat 5:126-30. 2002
    ..falciparum is described. Future possibilities in this field are also outlined in the light of recent technological advances...
  4. pmc Plasmodium falciparum virulence determinants unveiled
    Brendan S Crabb
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia
    Genome Biol 3:REVIEWS1031. 2002
    ..We must understand the functions of these elements if the chronicity and unpredictable virulence of Plasmodium is to be explained...
  5. ncbi request reprint A new system for rapid plasmid integration in Plasmodium parasites
    Brendan S Crabb
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne 3050, Australia
    Trends Microbiol 15:3-6. 2007
    ..In this article, we outline the potential of this system, describing how it enables direct site-specific integration and the rapid generation of stably transformed populations that express uniform levels of introduced transgenes...
  6. pmc Promising functional readouts of immunity in a blood-stage malaria vaccine trial
    Brendan S Crabb
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia
    PLoS Med 2:e380. 2005
  7. ncbi request reprint A common cross-species function for the double epidermal growth factor-like modules of the highly divergent plasmodium surface proteins MSP-1 and MSP-8
    Damien R Drew
    Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, Victoria 3050, Australia
    J Biol Chem 279:20147-53. 2004
    ....
  8. pmc Evidence for a common role for the serine-type Plasmodium falciparum serine repeat antigen proteases: implications for vaccine and drug design
    Joanne E McCoubrie
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    Infect Immun 75:5565-74. 2007
    ..While SERA5 is to some extent further validated as a target for vaccine and drug development, our data suggest that the expression level of other serine-type SERAs is the only barrier to escape from anti-SERA5-specific interventions...
  9. ncbi request reprint A var gene promoter controls allelic exclusion of virulence genes in Plasmodium falciparum malaria
    Till S Voss
    The Walter and Eliza Hall Institute of Medical Research, Parkville 3050, Australia
    Nature 439:1004-8. 2006
    ..falciparum. Furthermore, the PfEMP1 knockdown parasites obtained in this study will be important tools to increase our understanding of P. falciparum-mediated virulence and immune evasion...
  10. doi request reprint Blood-stage Plasmodium berghei infection leads to short-lived parasite-associated antigen presentation by dendritic cells
    Rachel J Lundie
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    Eur J Immunol 40:1674-81. 2010
    ..This provides important evidence that P. berghei not only causes immunosuppression to subsequently encountered third party antigens, but also rapidly limits the capacity to generate effective parasite-specific immunity...
  11. ncbi request reprint Distinct protein classes including novel merozoite surface antigens in Raft-like membranes of Plasmodium falciparum
    Paul R Sanders
    The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050 Australia
    J Biol Chem 280:40169-76. 2005
    ..This insight into merozoite surfaces provides new opportunities for understanding both erythrocyte invasion and anti-parasite immunity...
  12. doi request reprint MSP1(19) miniproteins can serve as targets for invasion inhibitory antibodies in Plasmodium falciparum provided they contain the correct domains for cell surface trafficking
    Paul R Gilson
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3050, Australia
    Mol Microbiol 68:124-38. 2008
    ..Using a line expressing a green fluorescent protein-MSP1 fusion protein, we demonstrate that one mode of inhibition by antibodies targeting the MSP1(19) domain is the rapid agglutinating of merozoites prior to erythrocyte attachment...
  13. ncbi request reprint Evidence that Plasmodium falciparum chromosome end clusters are cross-linked by protein and are the sites of both virulence gene silencing and activation
    Allison J Marty
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Mol Microbiol 62:72-83. 2006
    ..Our findings support a model whereby a highly localized structure dedicated to the activation of a single var gene can be housed within a gene dense chromosome end cluster that is otherwise transcriptionally silent...
  14. pmc Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes
    David T Riglar
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
    Nat Commun 4:1415. 2013
    ..These data provide the most direct cellular evidence to date that protein export occurs at regions of the parasitophorous vacuole membrane housing the Plasmodium translocon of exported proteins complex...
  15. pmc A newly discovered protein export machine in malaria parasites
    Tania F de Koning-Ward
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3052, Australia
    Nature 459:945-9. 2009
    ..Two other proteins, a new protein PTEX88 and thioredoxin 2 (TRX2), were also identified as PTEX components. As a common portal for numerous crucial processes, this translocon offers a new avenue for therapeutic intervention...
  16. pmc An aspartyl protease directs malaria effector proteins to the host cell
    Justin A Boddey
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3052, Australia
    Nature 463:627-31. 2010
    ..Identification of the protease responsible for export into erythrocytes provides a novel target for therapeutic intervention against this devastating disease...
  17. ncbi request reprint Heterochromatin silencing and locus repositioning linked to regulation of virulence genes in Plasmodium falciparum
    Manoj T Duraisingh
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Cell 121:13-24. 2005
    ..Together, this implies that locus repositioning and heterochromatic silencing play important roles in the epigenetic regulation of virulence genes in P. falciparum...
  18. pmc Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays
    Danny W Wilson
    Infection and Immunity Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria, 3050, Australia
    Malar J 9:152. 2010
    ..However, several constraints have limited the use of these assays in large-scale population studies, vaccine trials and compound screening for drug discovery and development...
  19. ncbi request reprint Identification of protein complexes in detergent-resistant membranes of Plasmodium falciparum schizonts
    Paul R Sanders
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Melbourne, VIC 3050, Australia
    Mol Biochem Parasitol 154:148-57. 2007
    ..Confirmation of MSP-1 oligomerization, together with the isolation of a number of known complexes by BN-PAGE, makes it highly likely that novel interactions occur amongst members of this proteome...
  20. pmc The Plasmodium falciparum erythrocyte invasion ligand Pfrh4 as a target of functional and protective human antibodies against malaria
    Linda Reiling
    Burnet Institute of Medical Research and Public Health, Victoria, Australia
    PLoS ONE 7:e45253. 2012
    ..Plasmodium falciparum reticulocyte-binding-homologue-4 (PfRh4) is important for invasion of human erythrocytes and may therefore be a target of protective immunity...
  21. pmc Protein kinase a dependent phosphorylation of apical membrane antigen 1 plays an important role in erythrocyte invasion by the malaria parasite
    Kerstin Leykauf
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    PLoS Pathog 6:e1000941. 2010
    ..In addition to shedding unexpected new light on AMA1 function, this work represents the first time PKA has been implicated in merozoite invasion...
  22. ncbi request reprint Identification and stoichiometry of glycosylphosphatidylinositol-anchored membrane proteins of the human malaria parasite Plasmodium falciparum
    Paul R Gilson
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Mol Cell Proteomics 5:1286-99. 2006
    ..Together this work identified 11 and predicted a further 19 GPI-anchored proteins in P. falciparum...
  23. ncbi request reprint The Plasmodium falciparum genome--a blueprint for erythrocyte invasion
    Alan F Cowman
    Walter and Eliza Hall Institute of Medical Research, PO Royal Melbourne Hospital, Melbourne, Victoria 3050, Australia
    Science 298:126-8. 2002
    ..The genome sequence of this parasite reveals new gene families encoding proteins that appear to mediate erythrocyte invasion...
  24. pmc Blood-stage Plasmodium infection induces CD8+ T lymphocytes to parasite-expressed antigens, largely regulated by CD8alpha+ dendritic cells
    Rachel J Lundie
    Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
    Proc Natl Acad Sci U S A 105:14509-14. 2008
    ..Finally, using three different experimental approaches, we provide evidence that CTL specific for parasite-expressed antigens contribute to ECM...
  25. ncbi request reprint VAR2CSA is the principal ligand for chondroitin sulfate A in two allogeneic isolates of Plasmodium falciparum
    Michael F Duffy
    The Department of Medicine, The University of Melbourne, The Royal Melbourne Hospital, Parkville, VIC 3050, Australia
    Mol Biochem Parasitol 148:117-24. 2006
    ....
  26. pmc Exported proteins required for virulence and rigidity of Plasmodium falciparum-infected human erythrocytes
    Alexander G Maier
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Cell 134:48-61. 2008
    ..Collectively these proteins function as a pathogen secretion system, similar to bacteria and may provide targets for antivirulence based therapies to a disease responsible for millions of deaths annually...
  27. pmc IP-10-mediated T cell homing promotes cerebral inflammation over splenic immunity to malaria infection
    Catherine Q Nie
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    PLoS Pathog 5:e1000369. 2009
    ..Thus, our results demonstrate that modulating homing of cellular immune responses to malaria is critical for reaching a balance between protective immunity and immunopathogenesis...
  28. pmc A novel family of Apicomplexan glideosome-associated proteins with an inner membrane-anchoring role
    Hayley E Bullen
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    J Biol Chem 284:25353-63. 2009
    ..Hence, these proteins are strong candidates for an IMC-anchoring role, either directly or indirectly tethering the motor to the cytoskeleton...
  29. doi request reprint Truncation of Plasmodium berghei merozoite surface protein 8 does not affect in vivo blood-stage development
    Tania F de Koning-Ward
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
    Mol Biochem Parasitol 159:69-72. 2008
    ..These data demonstrate that a full-length membrane-associated form of PbMSP8 is not essential for blood-stage growth...
  30. pmc Plasmodium falciparum merozoite surface protein 8 is a ring-stage membrane protein that localizes to the parasitophorous vacuole of infected erythrocytes
    Damien R Drew
    The Walter and Eliza Hall Institute for Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
    Infect Immun 73:3912-22. 2005
    ..However, it is still possible that MSP-8 is involved in an early parasitophorous vacuole function that is significant for pathogenesis in the human host...
  31. doi request reprint Potential epigenetic regulatory proteins localise to distinct nuclear sub-compartments in Plasmodium falciparum
    Jennifer Volz
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, VIC 3050, Australia
    Int J Parasitol 40:109-21. 2010
    ..These analyses demonstrated an ordered compartmentalisation, indicating a complex sub-nuclear organisation that contributes to the complexity of transcriptional regulation in P. falciparum...
  32. pmc Towards a vaccine against Plasmodium vivax malaria
    James G Beeson
    Infection and Immunity Division, Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    PLoS Med 4:e350. 2007
  33. ncbi request reprint A subset of Plasmodium falciparum SERA genes are expressed and appear to play an important role in the erythrocytic cycle
    Susanne K Miller
    Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    J Biol Chem 277:47524-32. 2002
    ..The inability to disrupt SERA4, -5, and -6 is consistent with their apparent dominant expression and implies an important role for these genes in maintenance of the erythrocytic cycle...
  34. ncbi request reprint Enzymic, phylogenetic, and structural characterization of the unusual papain-like protease domain of Plasmodium falciparum SERA5
    Anthony N Hodder
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, 1G Royal Parade, Parkville, Victoria 3050, Australia
    J Biol Chem 278:48169-77. 2003
    ..This study provides impetus for the examination of SERA5 as a target for antimalarial drug design...
  35. pmc Identification and prioritization of merozoite antigens as targets of protective human immunity to Plasmodium falciparum malaria for vaccine and biomarker development
    Jack S Richards
    Department of Immunology, Burnet Institute, Melbourne 3001, Victoria, Australia
    J Immunol 191:795-809. 2013
    ....
  36. pmc Alterations in local chromatin environment are involved in silencing and activation of subtelomeric var genes in Plasmodium falciparum
    Till S Voss
    Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Parkville 3050, Australia
    Mol Microbiol 66:139-50. 2007
    ....
  37. pmc Inhibition of malaria parasite development by a cyclic peptide that targets the vital parasite protein SERA5
    W Douglas Fairlie
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    Infect Immun 76:4332-44. 2008
    ..These data implicate an important role for SERA5 in the regulation of the intraerythrocytic development of late-stage parasites and as a target for drug development...
  38. pmc Biosynthesis, localization, and macromolecular arrangement of the Plasmodium falciparum translocon of exported proteins (PTEX)
    Hayley E Bullen
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    J Biol Chem 287:7871-84. 2012
    ..Collectively, these data support the hypothesis that EXP2 oligomerizes and potentially forms the putative membrane-spanning pore to which the remainder of the PTEX complex is attached...
  39. doi request reprint Structural insights into the protease-like antigen Plasmodium falciparum SERA5 and its noncanonical active-site serine
    Anthony N Hodder
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia
    J Mol Biol 392:154-65. 2009
    ..Attempts to determine the structure in complex with known inhibitors were not successful. Thus, despite having revealed its structure, the function of the catalytic domain of SERA5 remains an enigma...
  40. pmc Identification and disruption of the gene encoding the third member of the low-molecular-mass rhoptry complex in Plasmodium falciparum
    Deborah L Baldi
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Infect Immun 70:5236-45. 2002
    ..falciparum; however, there is a single gene. Phylogenetic comparison of the RAP2/3 protein family from Plasmodium spp. suggests that the RAP2/3 duplication occurred after divergence of these parasite species...
  41. pmc A set of glycosylphosphatidyl inositol-anchored membrane proteins of Plasmodium falciparum is refractory to genetic deletion
    Paul R Sanders
    Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
    Infect Immun 74:4330-8. 2006
    ..No distinguishable growth rate or invasion pathway phenotype was detected for the msp-5 knockout line, although its presence as a surface-localized protein was confirmed...
  42. doi request reprint Recent insights into the export of PEXEL/HTS-motif containing proteins in Plasmodium parasites
    Hayley E Bullen
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    Curr Opin Microbiol 15:699-704. 2012
    ..Roles for parasite-specific molecular processes in two distinct sites, the endoplasmic reticulum and the PVM have been revealed...
  43. pmc Biochemical and functional analysis of two Plasmodium falciparum blood-stage 6-cys proteins: P12 and P41
    Tana Taechalertpaisarn
    Burnet Institute, Melbourne, Victoria, Australia
    PLoS ONE 7:e41937. 2012
    ..It now appears likely that these blood-stage 6-cys proteins operate as a pair and play redundant roles either in erythrocyte invasion or in host-immune interactions...
  44. pmc Quantifying the importance of MSP1-19 as a target of growth-inhibitory and protective antibodies against Plasmodium falciparum in humans
    Danny W Wilson
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia
    PLoS ONE 6:e27705. 2011
    ..Merozoite surface protein 1 (MSP1) is an abundant and essential protein. The C-terminal 19 kDa region (MSP1-19) is regarded as a promising vaccine candidate and may also be an important target of immunity...
  45. doi request reprint The Plasmodium translocon of exported proteins (PTEX) component thioredoxin-2 is important for maintaining normal blood-stage growth
    Kathryn Matthews
    School of Medicine, Deakin University, Waurn Ponds, Vic, 3216, Australia
    Mol Microbiol 89:1167-86. 2013
    ..Thus, while not essential for parasite survival, TRX2 may help to optimize PTEX activity. Importantly, the generation of TRX2 knockout parasites that display altered phenotypes provides a much-needed tool to dissect PTEX function. ..
  46. pmc Dendritic cell preactivation impairs MHC class II presentation of vaccines and endogenous viral antigens
    Louise J Young
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
    Proc Natl Acad Sci U S A 104:17753-8. 2007
    ..These observations have important implications for the design of prophylactic and therapeutic DC vaccines and contribute to the understanding of the mechanisms causing immunosuppression during systemic blood infections...
  47. doi request reprint NK cells and conventional dendritic cells engage in reciprocal activation for the induction of inflammatory responses during Plasmodium berghei ANKA infection
    Victoria Ryg-Cornejo
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3052, Australia
    Immunobiology 218:263-71. 2013
    ..DC depletion and genetic deletion of IL-12 dramatically reduced NK cell-mediated IFN-γ responses to malaria. Thus NK cells and DCs engage in reciprocal activation for the induction of inflammatory responses involved in severe malaria...
  48. pmc A genetic screen for improved plasmid segregation reveals a role for Rep20 in the interaction of Plasmodium falciparum chromosomes
    Rebecca A O'Donnell
    The Walter and Eliza Hall Institute of Medical Research, Victoria 3050, Australia
    EMBO J 21:1231-9. 2002
    ..This study implicates a direct role for Rep20 in the physical association of chromosome ends, which is a process that facilitates the generation of diversity in the terminally located P.falciparum virulence genes...
  49. pmc Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites
    Jake Baum
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia
    Nucleic Acids Res 37:3788-98. 2009
    ....
  50. ncbi request reprint Truncation of merozoite surface protein 3 disrupts its trafficking and that of acidic-basic repeat protein to the surface of Plasmodium falciparum merozoites
    Kerry E Mills
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Mol Microbiol 43:1401-11. 2002
    ..These results suggest that MSP3 is not absolutely essential for blood stage growth and that the putative leucine zipper region is required for the trafficking of both MSP3 and ABRA to the parasitophorous vacuole...
  51. doi request reprint Blood-stage Plasmodium berghei infection generates a potent, specific CD8+ T-cell response despite residence largely in cells lacking MHC I processing machinery
    Lei Shong Lau
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia
    J Infect Dis 204:1989-96. 2011
    ..Our studies indicate that vigorous CTL responses can be induced to pathogens even when they largely reside in red blood cells, which lack MHC I processing machinery...
  52. doi request reprint Recruitment of Factor H as a Novel Complement Evasion Strategy for Blood-Stage Plasmodium falciparum Infection
    Alexander T Kennedy
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3052, Australia Department of Medical Biology, The University of Melbourne, Parkville, Victoria 3052, Australia
    J Immunol 196:1239-48. 2016
    ..Our study provides new insights on mechanisms of immune evasion of malaria parasites and highlights the important function of surface coat proteins in the interplay between complement regulation and successful infection of the host. ..
  53. pmc Revealing the sequence and resulting cellular morphology of receptor-ligand interactions during Plasmodium falciparum invasion of erythrocytes
    Greta E Weiss
    Burnet Institute, Melbourne, Australia
    PLoS Pathog 11:e1004670. 2015
    ..In addition to enhancing general knowledge of apicomplexan biology, this work provides a rational basis to combine sequentially acting merozoite vaccine candidates in a single multi-receptor-blocking vaccine. ..
  54. doi request reprint PTEX is an essential nexus for protein export in malaria parasites
    Brendan Elsworth
    1 Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, 3004, Australia 2 Monash University, Clayton, Victoria, 3800, Australia 3
    Nature 511:587-91. 2014
    ..Hence, as the only known nexus for protein export in Plasmodium parasites, and an essential enzymic machine, PTEX is a prime drug target. ..
  55. doi request reprint Acquisition of invasion-inhibitory antibodies specific for the 19-kDa fragment of merozoite surface protein 1 in a transmigrant population requires multiple infections
    E Elsa Herdiana Murhandarwati
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    J Infect Dis 198:1212-8. 2008
    ..This delay in the acquisition of functional antibodies by residents of areas where malaria is endemic is consistent with the observation that multiple malaria infections are required before clinical immunity is acquired...
  56. doi request reprint Proteomic analysis reveals novel proteins associated with the Plasmodium protein exporter PTEX and a loss of complex stability upon truncation of the core PTEX component, PTEX150
    Brendan Elsworth
    Burnet Institute, 85 Commercial Road, Melbourne, Vic, 3004, Australia
    Cell Microbiol 18:1551-1569. 2016
    ....
  57. pmc Conditional expression of apical membrane antigen 1 in Plasmodium falciparum shows it is required for erythrocyte invasion by merozoites
    Alan Yap
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Vic, 3052, Australia
    Cell Microbiol 16:642-56. 2014
    ..These results show that PfAMA1 is an essential protein for merozoite invasion in P.  falciparum and either directly or indirectly plays a role in resealing of the red blood cell at the posterior end of the invasion event...
  58. pmc Plasmodium falciparum transfected with ultra bright NanoLuc luciferase offers high sensitivity detection for the screening of growth and cellular trafficking inhibitors
    Mauro F Azevedo
    Macfarlane Burnet Institute of Medical Research and Public Health, Melbourne, Victoria, Australia
    PLoS ONE 9:e112571. 2014
    ....
  59. doi request reprint Inhibition of Plasmodium falciparum CDPK1 by conditional expression of its J-domain demonstrates a key role in schizont development
    Mauro F Azevedo
    Centre for Immunology, Macfarlane Burnet Institute of Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    Biochem J 452:433-41. 2013
    ..These data point to an early schizont function for PfCDPK1 and demonstrate that conditionally expressing auto-inhibitory regions can be an effective way to address the function of Plasmodium enzymes...
  60. ncbi request reprint The malaria parasite Plasmodium falciparum has only one pyruvate dehydrogenase complex, which is located in the apicoplast
    Bernardo J Foth
    Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, VIC 3010, Australia
    Mol Microbiol 55:39-53. 2005
    ..The recombinantly expressed catalytic domain of the PDH subunit E2 showed high enzymatic activity in vitro indicating that pyruvate is converted to acetyl-CoA in the apicoplast, possibly for use in fatty acid biosynthesis...
  61. ncbi request reprint Transfection of the human malaria parasite Plasmodium falciparum
    Brendan S Crabb
    Division of Infection and Immunity, The Walter and Eliza Hall Institute of Medical Research, Victoria, Australia
    Methods Mol Biol 270:263-76. 2004
    ..The different plasmid vectors that are currently used for gene targeting and transgene expression (including green fluorescent protein expression) are also described...
  62. ncbi request reprint The role of osmiophilic bodies and Pfg377 expression in female gametocyte emergence and mosquito infectivity in the human malaria parasite Plasmodium falciparum
    Tania F de Koning-Ward
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, VIC 3050, Australia
    Mol Microbiol 67:278-90. 2008
    ..This reduced efficiency of emergence explains the significant defect in oocyst formation in mosquitoes fed blood meals containing Pfg377-negative gametocytes, resulting in an almost complete blockade of infection...
  63. pmc Tetracycline analogue-regulated transgene expression in Plasmodium falciparum blood stages using Toxoplasma gondii transactivators
    Markus Meissner
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville VIC 3050, Australia
    Proc Natl Acad Sci U S A 102:2980-5. 2005
    ..On the other hand, the identified transactivators are not functional in mammalian cells, consistent with the fundamental differences in the mechanism of gene regulation between Apicomplexan parasites and their human hosts...
  64. pmc Functional analysis of Plasmodium falciparum merozoite antigens: implications for erythrocyte invasion and vaccine development
    Alan F Cowman
    The Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria 3050, Australia
    Philos Trans R Soc Lond B Biol Sci 357:25-33. 2002
    ..This has provided new information on the role of merozoite antigens in erythrocyte invasion and also allows new approaches to address their potential as vaccine candidates...
  65. ncbi request reprint Invasion of red blood cells by malaria parasites
    Alan F Cowman
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Cell 124:755-66. 2006
    ..While some Apicomplexa can invade many different host cells, the disease-associated blood-stage form of the malaria parasite is restricted to erythrocytes...
  66. pmc Novel roles for erythroid Ankyrin-1 revealed through an ENU-induced null mouse mutant
    Gerhard Rank
    Rotary Bone Marrow Research Laboratory, Melbourne Health Research Directorate, Parkville, Australia
    Blood 113:3352-62. 2009
    ..These findings provide novel insights into the role of Ank-1, and define an ideal model for the study of HS and malarial resistance...
  67. pmc Association of iss and iucA, but not tsh, with plasmid-mediated virulence of avian pathogenic Escherichia coli
    Kelly A Tivendale
    Department of Veterinary Science, The University of Melbourne, Parkville, 3010 Victoria, Australia
    Infect Immun 72:6554-60. 2004
    ..coli infection in other species suggests that they may be useful models for definition of the role of these virulence genes and of other novel virulence genes that may be located on their virulence plasmids...
  68. doi request reprint Morphology and kinetics of the three distinct phases of red blood cell invasion by Plasmodium falciparum merozoites
    Paul R Gilson
    Walter and Eliza Hall Institute of Medical Research, Parkville, VIC 3050, Australia
    Int J Parasitol 39:91-6. 2009
    ..This study provides a morphological and kinetic framework onto which the invasion-associated physiological and molecular events can be overlaid...
  69. pmc Toward forward genetic screens in malaria-causing parasites using the piggyBac transposon
    Brendan S Crabb
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria 3004, Australia
    BMC Biol 9:21. 2011
    ..But further improvements are still needed for the full power of genome-wide molecular genetic screens to be utilized in this organism.See research article: http://www.biomedcentral.com/1471-2164/12/155...
  70. ncbi request reprint Systemic activation of dendritic cells by Toll-like receptor ligands or malaria infection impairs cross-presentation and antiviral immunity
    Nicholas S Wilson
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia
    Nat Immunol 7:165-72. 2006
    ..Moreover, inhibition of cross-presentation was overcome by injection of dendritic cells bearing antigen, which provides a new strategy for generating immunity during immunosuppressive blood infections...
  71. doi request reprint A model for the progression of receptor-ligand interactions during erythrocyte invasion by Plasmodium falciparum
    Katherine L Harvey
    Burnet Institute, Melbourne, Victoria 3004, Australia
    Int J Parasitol 42:567-73. 2012
    ..This review provides an update on the current details of these interactions to place them in sequence and super impose them upon the known kinetic events of invasion...
  72. ncbi request reprint Revealing the molecular determinants of gender in malaria parasites
    Alan F Cowman
    The Walter and Eliza Hall Institute of Medical Research, Melbourne 3050, Australia
    Cell 121:659-60. 2005
    ..In this issue of Cell, Khan et al. (2005) used elegant approaches to purify male and female gametocytes and elucidated their respective proteome, providing the basis for understanding sexual development in this pathogen...
  73. ncbi request reprint Functional genomics: identifying drug targets for parasitic diseases
    Alan F Cowman
    The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Melbourne 3050, Australia
    Trends Parasitol 19:538-43. 2003
  74. pmc Embryonic age influences the capacity for cytokine induction in chicken thymocytes
    Michelle A Peters
    Department of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia
    Immunology 110:358-67. 2003
    ..The balance of responses in the embryonic thymus suggests that at all stages thymocytes have a reduced capacity for activation in comparison to mature thymocyte populations...
  75. ncbi request reprint CD1d-restricted NKT cells contribute to malarial splenomegaly and enhance parasite-specific antibody responses
    Diana S Hansen
    The Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria, Australia
    Eur J Immunol 33:2588-98. 2003
    ....
  76. ncbi request reprint Molecular approaches to malaria: MAM 2004 and beyond
    Brendan S Crabb
    Trends Parasitol 20:547. 2004
    ..Rather, the articles address, in more general terms, recent advances on broader themes that were prominent at Molecular Approaches to Malaria meeting 2004...
  77. pmc The fine specificity, but not the invasion inhibitory activity, of 19-kilodalton merozoite surface protein 1-specific antibodies is associated with resistance to malarial parasitemia in a cross-sectional survey in The Gambia
    Patrick H Corran
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, United Kingdom
    Infect Immun 72:6185-9. 2004
    ..10) were significantly less likely to have malaria infections with densities of > or =1,000 parasites/microl than were individuals without such antibodies. This association persisted after correction for age and ethnic origin...
  78. ncbi request reprint The serine repeat antigen (SERA) gene family phylogeny in Plasmodium: the impact of GC content and reconciliation of gene and species trees
    Richard Bourgon
    Department of Statistics, University of California, Berkeley, USA
    Mol Biol Evol 21:2161-71. 2004
    ..Parsimony of reconciliation and a second criterion--implied mutational pattern at two key active sites in the SERA proteins-were also seen to be useful supplements to standard "bootstrap" analysis for inferred topologies...
  79. ncbi request reprint Evidence that invasion-inhibitory antibodies specific for the 19-kDa fragment of merozoite surface protein-1 (MSP-1 19) can play a protective role against blood-stage Plasmodium falciparum infection in individuals in a malaria endemic area of Africa
    Chandy C John
    Center for Global Health and Diseases, Case Western Reserve University, Wolstein Research Building, 2103 Cornell Road, Cleveland, OH 44106, USA
    J Immunol 173:666-72. 2004
    ..falciparum infection, and suggest that the measurement of MSP-1(19) specific inhibitory Abs may serve as an accurate correlate of protection in clinical trials of MSP-1-based vaccines...
  80. ncbi request reprint A parasite genome sheds light on an old enemy
    Alan F Cowman
    Nat Biotechnol 20:1098-9. 2002
  81. pmc Comparative genomics of the neglected human malaria parasite Plasmodium vivax
    Jane M Carlton
    The Institute for Genomic Research J Craig Venter Institute, 9704 Medical Research Drive, Rockville, Maryland 20850, USA
    Nature 455:757-63. 2008
    ..Completion of the P. vivax genome provides the scientific community with a valuable resource that can be used to advance investigation into this neglected species...
  82. pmc Several recombinant capsid proteins of equine rhinitis a virus show potential as diagnostic antigens
    Fan Li
    Centre for Equine Virology, School of Veterinary Science, The University of Melbourne, Victoria 3010, Australia
    Clin Diagn Lab Immunol 12:778-85. 2005
    ..This study provides promising candidates for development of a diagnostic ERAV ELISA...
  83. pmc Trafficking of STEVOR to the Maurer's clefts in Plasmodium falciparum-infected erythrocytes
    Jude M Przyborski
    Hygiene Institut, Abteilung Parasitologie, Universitatsklinikum Heidelberg, Heidelberg, Germany
    EMBO J 24:2306-17. 2005
    ..Our data suggest general principles for transport of transmembrane proteins to the Maurer's clefts and provide new insights into protein sorting and trafficking processes in P. falciparum...
  84. ncbi request reprint Immunogenicity and protective efficacy of Escherichia coli expressed Plasmodium falciparum merozoite surface protein-1(42) using human compatible adjuvants
    Suraksha Sachdeva
    International Centre for Genetic Engineering and Biotechnology, Aruna Asaf Ali Marg, New Delhi 110067, India
    Vaccine 24:2007-16. 2006
    ..berghei/P. falciparum chimeric line (Pb-PfM19) that expresses Plasmodium falciparum MSP-1(19). These findings may be useful for the development of a malaria vaccine based on Plasmodium falciparum MSP-1(42)...
  85. ncbi request reprint Prenatal malaria immune experience affects acquisition of Plasmodium falciparum merozoite surface protein-1 invasion inhibitory antibodies during infancy
    Arlene Dent
    Center for Global Health and Diseases, Case Western Reserve University, Cleveland, OH 44106, USA
    J Immunol 177:7139-45. 2006
    ..These data suggest that fetal sensitization or tolerance to MSP-1, associated with maternal malaria infection during pregnancy, affects the development of functional Ab responses to MSP-1 during infancy...
  86. pmc The importance of human FcgammaRI in mediating protection to malaria
    Richard S McIntosh
    Institute of Genetics, Queen s Medical Centre, University of Nottingham, United Kingdom
    PLoS Pathog 3:e72. 2007
    ..This important finding documents the capacity of FcgammaRI to mediate potent antimalaria immunity and supports the development of FcgammaRI-directed therapy for human malaria...
  87. ncbi request reprint Molecular genetic tools in Toxoplasma and Plasmodium: achievements and future needs
    Markus Meissner
    Hygieneinstitut Heidelberg, Abteilung Parasitologie, Universitatsklinikum Heidelberg, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Curr Opin Microbiol 10:349-56. 2007
    ..This review summarises the methodologies available for functional gene analysis in apicomplexan parasites and discusses further needs in tool development...
  88. ncbi request reprint Site-directed mutagenesis of the VP2 gene of Chicken anemia virus affects virus replication, cytopathology and host-cell MHC class I expression
    Michelle A Peters
    Department of Veterinary Science, The University of Melbourne, Victoria 3010, Australia
    J Gen Virol 87:823-31. 2006
    ....
  89. ncbi request reprint Attenuation of chicken anemia virus by site-directed mutagenesis of VP2
    Michelle A Peters
    Department of Veterinary Science, The University of Melbourne, Victoria 3010, Australia
    J Gen Virol 88:2168-75. 2007
    ..These findings show that mutations in the VP2 gene can reduce the virulence of CAV and these mutant viruses may have value as vaccine candidates...
  90. ncbi request reprint Chicken anemia virus VP2 is a novel dual specificity protein phosphatase
    Michelle A Peters
    Department of Veterinary Science, University of Melbourne, Victoria 3010, Australia
    J Biol Chem 277:39566-73. 2002
    ..This is the first DSP gene to be identified in a small viral genome...