Genomes and Genes
Ingrid G Winkler
Affiliation: South Brisbane
- Vascular niche E-selectin regulates hematopoietic stem cell dormancy, self renewal and chemoresistanceIngrid G Winkler
Stem Cells and Cancer Group, Mater Medical Research Institute, South Brisbane, Queensland, Australia
Nat Med 18:1651-7. 2012..As bone marrow suppression is a severe side effect of high-dose chemotherapy, transient blockade of E-selectin is potentially a promising treatment for the protection of HSCs during chemotherapy or irradiation...
- Serine protease inhibitors serpina1 and serpina3 are down-regulated in bone marrow during hematopoietic progenitor mobilizationIngrid G Winkler
Haematopoietic Stem Cell Laboratory, Mater Medical Research Institute, South Brisbane, Queensland 4101, Australia
J Exp Med 201:1077-88. 2005..These data suggest an unexpected role for serpina1 and serpina3 in regulating the bone marrow hematopoietic microenvironment as well as influencing the migratory behavior of hematopoietic precursors...
- Flow cytometry measurement of bone marrow perfusion in the mouse and sorting of progenitors and stems cells according to position relative to blood flow in vivoValerie Barbier
Stem Cell and Cancer Group, Mater Medical Research Institute, South Brisbane, QLD, Australia
Methods Mol Biol 844:45-63. 2012..This technique enables prospective isolation of HSCs based on the in vivo perfusion of the niches in which they reside...
- Positioning of bone marrow hematopoietic and stromal cells relative to blood flow in vivo: serially reconstituting hematopoietic stem cells reside in distinct nonperfused nichesIngrid G Winkler
Biotherapy Program, Mater Medical Research Institute, South Brisbane, Australia
Blood 116:375-85. 2010..This technique enables prospective isolation of serially reconstituting HSCs distinct from other less potent HSCs of the same phenotype, based on the in vivo niche in which they reside...
- B-lymphopoiesis is stopped by mobilizing doses of G-CSF and is rescued by overexpression of the anti-apoptotic protein Bcl2Ingrid G Winkler
Mater Research at the Translational Research Institute, Woolloongabba, Queensland, Australia
Haematologica 98:325-33. 2013..These results suggest that blood mobilized with these three agents may have distinct immune properties...
- Hematopoietic progenitor cell mobilization results in hypoxia with increased hypoxia-inducible transcription factor-1 alpha and vascular endothelial growth factor A in bone marrowJean Pierre Levesque
Mater Medical Research Institute, Raymond Terrace, Aubigny Place, South Brisbane, QLD, Australia
Stem Cells 25:1954-65. 2007..Disclosure of potential conflicts of interest is found at the end of this article...
- Bone marrow macrophages maintain hematopoietic stem cell (HSC) niches and their depletion mobilizes HSCsIngrid G Winkler
Haematopoietic Stem Cell Laboratory, Mater Medical Research Institute, South Brisbane, Australia
Blood 116:4815-28. 2010..Together, these results establish that bone marrow macrophages are pivotal to maintain the endosteal HSC niche and that the loss of such macrophages leads to the egress of HSCs into the blood...
- Mobilization of hematopoietic stem cells by depleting bone marrow macrophagesValerie Barbier
Mater Medical Research Institute, Aubigny Place, Raymond Terrace, South Brisbane, QLD, Australia
Methods Mol Biol 904:117-38. 2012..Detailed methods to analyze mobilization of phenotypic and functional reconstituting HSC are described with examples...
- Flow cytometry analysis of cell cycling and proliferation in mouse hematopoietic stem and progenitor cellsValerie Barbier
Stem Cell and Cancer Group, Mater Medical Research Institute, South Brisbane, QLD, Australia
Methods Mol Biol 844:31-43. 2012....
- Mobilization with granulocyte colony-stimulating factor blocks medullar erythropoiesis by depleting F4/80(+)VCAM1(+)CD169(+)ER-HR3(+)Ly6G(+) erythroid island macrophages in the mouseRebecca N Jacobsen
Stem Cell Biology Group, Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia School of Medicine, University of Queensland, Saint Lucia, Queensland, Australia
Exp Hematol 42:547-61.e4. 2014..Agents that affect these macrophages, such as G-CSF, are likely to affect these three processes concomitantly. ..
- Pharmacologic stabilization of HIF-1α increases hematopoietic stem cell quiescence in vivo and accelerates blood recovery after severe irradiationCatherine E Forristal
Stem Cell Biology Group, Mater Medical Research Institute, South Brisbane, Australia
Blood 121:759-69. 2013..The results of the present study demonstrate that the levels of HIF-1α protein can be manipulated pharmacologically in vivo to increase HSC quiescence and recovery from irradiation...
- Nichotherapy for stem cells: there goes the neighborhoodJean Pierre Levesque
Stem Cell Biology Group, Biological Therapies Program, Mater Medical Research Institute, South Brisbane, Australia
Bioessays 35:183-90. 2013..Some have been employed for decades, such as hematopoietic stem cell mobilization, whereas others are emerging, such as chemosensitization of leukemia stem cells by targeting their niche...
- Mechanisms of hematopoietic stem cell mobilization: when innate immunity assails the cells that make blood and boneIngrid G Winkler
Haematopoietic Stem Cell Laboratory, Cancer Biotherapies Program, Mater Medical Research Institute, University of Queensland, South Brisbane, Queensland, Australia
Exp Hematol 34:996-1009. 2006....
- 38th Annual Meeting of the International Society of Experimental HematologyJean Pierre Levesque
Haematopoietic Stem Cell Laboratory, Mater Medical Research Institute, Aubigny Place, Raymond Terrace, South Brisbane, QLD 4101, Australia
Expert Rev Hematol 2:645-7. 2009..Owing to the large number of keynote speakers, this report focuses on the most novel, unpublished data presented during the meeting...
- Tissue inhibitor of metalloproteinase-3 (TIMP-3) regulates hematopoiesis and bone formation in vivoYi Shen
Hematopoietic Stem Cell Laboratory, Mater Medical Research Institute, South Brisbane, Queensland, Australia
PLoS ONE 5:. 2010..TIMP-3 is associated with the extracellular matrix and is expressed in highly remodeling tissues. TIMP-3 function in the hematopoietic system is unknown...
- Mobilization of hematopoietic stem cells: state of the artJean Pierre Levesque
Mater Medical Research Institute, South Brisbane, Queensland, Australia
Curr Opin Organ Transplant 13:53-8. 2008..This article reviews recent advances in understanding the molecular mechanisms responsible for the retention of HSCs in the bone marrow, which are perturbed during HSC mobilization, and the clinical application of these findings...
- Hierarchy of immature hematopoietic cells related to blood flow and nicheJean Pierre Levesque
Biological Therapies Program, Mater Medical Research Institute, South Brisbane, Australia
Curr Opin Hematol 18:220-5. 2011..Extrinsic instructions provided by unique microenvironments (niches) regulate the fate of individual HSCs and progenitors. This review discusses the latest findings in respect to the organization and function of these niches...
- A novel mouse model of veno-occlusive disease provides strategies to prevent thioguanine-induced hepatic toxicityIulia Oancea
IBD Team, Immunology Infection Inflammation Program, Mater Medical Research Institute, South Brisbane, Queensland, Australia
Gut 62:594-605. 2013..Understanding of SOS has been hindered by inability to ethically perform serial liver biopsies on patients and the lack of an animal model...
- Granulocyte colony-stimulating factor induces the release in the bone marrow of proteases that cleave c-KIT receptor (CD117) from the surface of hematopoietic progenitor cellsJean Pierre Levesque
Stem Cell Laboratory, Peter MacCallum Cancer Institute, Locked Bag 1, A Beckett Street, Melbourne, Victoria 3000, Australia
Exp Hematol 31:109-17. 2003..We sought to determine the mechanisms responsible for the reduced expression of c-KIT on mobilized hematopoietic progenitor cells...
- Granulocyte colony-stimulating factor and an RARalpha specific agonist, VTP195183, synergize to enhance the mobilization of hematopoietic progenitor cellsKirsten E Herbert
Peter MacCallum Cancer Centre, Melbourne, Victoria, Australia
Transplantation 83:375-84. 2007....
- Mobilization by either cyclophosphamide or granulocyte colony-stimulating factor transforms the bone marrow into a highly proteolytic environmentJean Pierre Levesque
Stem Cell Biology Laboratory, Peter MacCallum Cancer Institute, Melbourne, Victoria, Australia
Exp Hematol 30:440-9. 2002..We sought to determine whether mobilization induced by G-CSF or chemotherapy was triggered by common or distinct mechanisms...
- Detection of antiviral antibodies using enzyme-linked immunosorbent assayIngrid G Winkler
Methods Mol Biol 304:257-71. 2005..The second uses fixed "virus-infected" cultured cells and can be used either as a second confirmatory assay or initially in a small-scale screening to identify potentially infected individuals for further study...
- Adhesion to E-selectin promotes growth inhibition and apoptosis of human and murine hematopoietic progenitor cells independent of PSGL-1Ingrid G Winkler
Adhesive Interactions and Cell Trafficking Laboratory, Peter MacCallum Cancer Centre, Melbourne, Vic, Australia
Blood 103:1685-92. 2004..These data demonstrate that an E-selectin ligand(s) other than PSGL-1 transduces growth inhibitory and proapoptotic signals and requires posttranslational fucosylation to be functional...