Paul R Gorry

Summary

Affiliation: Macfarlane Burnet Institute for Medical Research and Public Health
Country: Australia

Publications

  1. ncbi request reprint Enhanced replicative capacity and pathogenicity of HIV-1 isolated from individuals infected with drug-resistant virus and declining CD4+ T-cell counts
    Ajantha Solomon
    Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria, Australia
    J Acquir Immune Defic Syndr 40:140-8. 2005
  2. pmc Distinct HIV-1 entry phenotypes are associated with transmission, subtype specificity, and resistance to broadly neutralizing antibodies
    Kelechi Chikere
    Department of Microbiology, Immunology, and Molecular Genetics, Los Angeles, USA
    Retrovirology 11:48. 2014
  3. pmc Macrophage Tropism and Cytopathicity of HIV-1 Variants Isolated Sequentially from a Long-Term Survivor Infected with nef-Deleted Virus
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Open Microbiol J 1:1-7. 2007
  4. pmc HIV-1 envelope-receptor interactions required for macrophage infection and implications for current HIV-1 cure strategies
    Paul R Gorry
    1 GPO Box 2284, Melbourne 3001, Victoria, Australia or University of Pennsylvania, 522 Johnson Pavilion, 36th and Hamilton Walk, Philadelphia, PA 19104 6060, USA E mail
    J Leukoc Biol 95:71-81. 2014
  5. pmc Linkages between HIV-1 specificity for CCR5 or CXCR4 and in vitro usage of alternative coreceptors during progressive HIV-1 subtype C infection
    Kieran Cashin
    Center for Biomedical Research, Burnet Institute, 85 Commercial Rd, Melbourne, Victoria 3004, Australia
    Retrovirology 10:98. 2013
  6. pmc Mechanisms of HIV non-progression; robust and sustained CD4+ T-cell proliferative responses to p24 antigen correlate with control of viraemia and lack of disease progression after long-term transfusion-acquired HIV-1 infection
    Wayne B Dyer
    Australian Red Cross Blood Service, 153 Clarence Street, Sydney, NSW 2000, Australia
    Retrovirology 5:112. 2008
  7. pmc Evolution of DC-SIGN use revealed by fitness studies of R5 HIV-1 variants emerging during AIDS progression
    Marie Borggren
    Dept Laboratory Medicine, Lund University, Lund, Sweden
    Retrovirology 5:28. 2008
  8. ncbi request reprint The role of viral coreceptors and enhanced macrophage tropism in human immunodeficiency virus type 1 disease progression
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Australia
    Sex Health 1:23-34. 2004
  9. doi request reprint Coreceptors and HIV-1 pathogenesis
    Paul R Gorry
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    Curr HIV/AIDS Rep 8:45-53. 2011
  10. ncbi request reprint Astrocyte infection by HIV-1: mechanisms of restricted virus replication, and role in the pathogenesis of HIV-1-associated dementia
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Curr HIV Res 1:463-73. 2003

Research Grants

  1. Pathogenesis of Macrophage Tropic HIV-1
    Paul Gorry; Fiscal Year: 2004

Detail Information

Publications83

  1. ncbi request reprint Enhanced replicative capacity and pathogenicity of HIV-1 isolated from individuals infected with drug-resistant virus and declining CD4+ T-cell counts
    Ajantha Solomon
    Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria, Australia
    J Acquir Immune Defic Syndr 40:140-8. 2005
    ..In summary, although drug resistance may lead to impaired viral fitness, the capacity of virus quasispecies from PBMCs to use CXCR4 may have significant consequences on viral replicative capacity and potentially on clinical outcome...
  2. pmc Distinct HIV-1 entry phenotypes are associated with transmission, subtype specificity, and resistance to broadly neutralizing antibodies
    Kelechi Chikere
    Department of Microbiology, Immunology, and Molecular Genetics, Los Angeles, USA
    Retrovirology 11:48. 2014
    ..This GGR Affinofile system enabled a higher throughput characterization of CD4/CCR5 usage efficiencies associated with differential Env phenotypes...
  3. pmc Macrophage Tropism and Cytopathicity of HIV-1 Variants Isolated Sequentially from a Long-Term Survivor Infected with nef-Deleted Virus
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Open Microbiol J 1:1-7. 2007
    ..Together, these results suggest that enhanced cytopathicity of nef-deleted HIV-1 for MDM can occur independently of replication capacity, and may contribute to the pathogenesis of nef-deleted HIV-1 infection...
  4. pmc HIV-1 envelope-receptor interactions required for macrophage infection and implications for current HIV-1 cure strategies
    Paul R Gorry
    1 GPO Box 2284, Melbourne 3001, Victoria, Australia or University of Pennsylvania, 522 Johnson Pavilion, 36th and Hamilton Walk, Philadelphia, PA 19104 6060, USA E mail
    J Leukoc Biol 95:71-81. 2014
    ....
  5. pmc Linkages between HIV-1 specificity for CCR5 or CXCR4 and in vitro usage of alternative coreceptors during progressive HIV-1 subtype C infection
    Kieran Cashin
    Center for Biomedical Research, Burnet Institute, 85 Commercial Rd, Melbourne, Victoria 3004, Australia
    Retrovirology 10:98. 2013
    ....
  6. pmc Mechanisms of HIV non-progression; robust and sustained CD4+ T-cell proliferative responses to p24 antigen correlate with control of viraemia and lack of disease progression after long-term transfusion-acquired HIV-1 infection
    Wayne B Dyer
    Australian Red Cross Blood Service, 153 Clarence Street, Sydney, NSW 2000, Australia
    Retrovirology 5:112. 2008
    ..We examined the mechanisms that differentiated delayed progressors from LTNP in this cohort...
  7. pmc Evolution of DC-SIGN use revealed by fitness studies of R5 HIV-1 variants emerging during AIDS progression
    Marie Borggren
    Dept Laboratory Medicine, Lund University, Lund, Sweden
    Retrovirology 5:28. 2008
    ..e. at the chronic stage and during end-stage disease, with regard to efficiency of DC-SIGN use in trans-infections...
  8. ncbi request reprint The role of viral coreceptors and enhanced macrophage tropism in human immunodeficiency virus type 1 disease progression
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Australia
    Sex Health 1:23-34. 2004
    ..These variants are likely to impact HIV-1 disease progression, especially in patients who persistently harbour only R5 viral strains...
  9. doi request reprint Coreceptors and HIV-1 pathogenesis
    Paul R Gorry
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    Curr HIV/AIDS Rep 8:45-53. 2011
    ..This review highlights recent literature that has advanced our understanding of the role of coreceptors in HIV-1 pathogenesis...
  10. ncbi request reprint Astrocyte infection by HIV-1: mechanisms of restricted virus replication, and role in the pathogenesis of HIV-1-associated dementia
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Curr HIV Res 1:463-73. 2003
    ....
  11. pmc Pathogenicity and immunogenicity of attenuated, nef-deleted HIV-1 strains in vivo
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Retrovirology 4:66. 2007
    ....
  12. ncbi request reprint Replication-dependent pathogenicity of attenuated nef-deleted HIV-1 in vivo
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    J Acquir Immune Defic Syndr 46:390-4. 2007
    ..We report the outcome of a 26-year prospective study documenting the clinical course of nef-deleted HIV-1 infection in 7 SBBC members...
  13. ncbi request reprint Pathogenesis of macrophage tropic HIV-1
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Curr HIV Res 3:53-60. 2005
    ..These variants are likely to impact HIV-1 disease progression, particularly in patients who persistently harbor only R5 viral strains...
  14. pmc Phenotype and envelope gene diversity of nef-deleted HIV-1 isolated from long-term survivors infected from a single source
    Lachlan Gray
    Macfarlane Burnet Institute for Medical Research and Public Health, Victoria, Australia
    Virol J 4:75. 2007
    ..To better understand mechanisms underlying the pathogenicity of nef-deleted HIV-1, we examined the phenotype and env sequence diversity of sequentially isolated viruses (n = 2) from 3 SBBC members...
  15. doi request reprint The magnitude of HIV-1 resistance to the CCR5 antagonist maraviroc may impart a differential alteration in HIV-1 tropism for macrophages and T-cell subsets
    Jacqueline K Flynn
    Center for Virology, Burnet Institute, Melbourne, Vic, Australia
    Virology 442:51-8. 2013
    ..The pattern of HIV-1 tropism alterations for susceptible cells may therefore be variable in subjects with MVC resistance...
  16. pmc CD4 and MHC class 1 down-modulation activities of nef alleles from brain- and lymphoid tissue-derived primary HIV-1 isolates
    Lachlan R Gray
    Center for Virology, Burnet Institute, 85 Commercial Rd, Melbourne, 3004 VIC, Australia
    J Neurovirol 17:82-91. 2011
    ....
  17. pmc Viral phenotypes and antibody responses in long-term survivors infected with attenuated human immunodeficiency virus type 1 containing deletions in the nef and long terminal repeat regions
    Erin E Verity
    Department of Microbiology, Monash University, Clayton, Victoria, Australia
    J Virol 81:9268-78. 2007
    ..Despite the presence of strong neutralizing antibodies in a number of SBBC members, disease progression was not prevented, and each cohort member studied displayed a unique outcome of infection with nef-attenuated HIV-1...
  18. pmc An altered and more efficient mechanism of CCR5 engagement contributes to macrophage tropism of CCR5-using HIV-1 envelopes
    Jasminka Sterjovski
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    Virology 404:269-78. 2010
    ..Thus, enhanced gp120-CCR5 interactions may contribute to M-tropism of R5 HIV-1 strains through different structural mechanisms...
  19. ncbi request reprint Uncoupling coreceptor usage of human immunodeficiency virus type 1 (HIV-1) from macrophage tropism reveals biological properties of CCR5-restricted HIV-1 isolates from patients with acquired immunodeficiency syndrome
    Lachlan Gray
    Macfarlane Burnet Institute for Medical Research and Public Health, GPO Box 2284, Melbourne, 3001 Victoria, Australia Department of Microbiology and Immunology, University of Melbourne, Victoria, Australia
    Virology 337:384-98. 2005
    ..Thus, reduced CD4/CCR5 dependence is a phenotype of R5 HIV-1 associated with M-tropism and late stage infection, which may affect the efficacy of HIV-1 entry inhibitors...
  20. pmc Asn 362 in gp120 contributes to enhanced fusogenicity by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with AIDS
    Jasminka Sterjovski
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Retrovirology 4:89. 2007
    ....
  21. doi request reprint Enhanced CD4+ cellular apoptosis by CCR5-restricted HIV-1 envelope glycoprotein variants from patients with progressive HIV-1 infection
    Jessica Wade
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    Virology 396:246-55. 2010
    ..Furthermore, they contribute to a better understanding of mechanisms involved in CD4+ T-cell loss in subjects experiencing progressive R5 HIV-1 infection...
  22. pmc Tissue-specific sequence alterations in the human immunodeficiency virus type 1 envelope favoring CCR5 usage contribute to persistence of dual-tropic virus in the brain
    Lachlan Gray
    Macfarlane Burnet Institute for Medical Research and Public Health, Victoria, Australia
    J Virol 83:5430-41. 2009
    ....
  23. doi request reprint Extensive astrocyte infection is prominent in human immunodeficiency virus-associated dementia
    Melissa J Churchill
    Centre for Virology, The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Ann Neurol 66:253-8. 2009
    ..Our data indicate that astrocytes can be extensively infected with HIV, and suggest an important role for HIV-infected astrocytes in HIV neuropathogenesis...
  24. doi request reprint Structural elements of primary CCR5-using HIV-1 gp120 proteins influencing sensitivity and resistance to the broadly neutralizing monoclonal antibody b12
    Jasminka Sterjovski
    Center for Virology, Burnet Institute, Melbourne, VIC 3004, Australia
    Virology 432:394-404. 2012
    ..Together, our studies provide new mechanistic insights into the gp120 determinants influencing sensitivity and resistance to HIV-1 neutralization by b12...
  25. pmc Alternative coreceptor requirements for efficient CCR5- and CXCR4-mediated HIV-1 entry into macrophages
    Kieran Cashin
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    J Virol 85:10699-709. 2011
    ..Together, the results of our study provide novel insights into alternative mechanisms of Env-coreceptor engagement that are associated with efficient CCR5- and CXCR4-mediated HIV-1 entry into macrophages...
  26. pmc HIV-1 escape from the CCR5 antagonist maraviroc associated with an altered and less-efficient mechanism of gp120-CCR5 engagement that attenuates macrophage tropism
    Michael Roche
    Centers for Virology, Monash University, Australia
    J Virol 85:4330-42. 2011
    ....
  27. doi request reprint CD4-binding site alterations in CCR5-using HIV-1 envelopes influencing gp120-CD4 interactions and fusogenicity
    Jasminka Sterjovski
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    Virology 410:418-28. 2011
    ..Our results provide evidence that the gp120 V5 loop may alter CD4bs conformation and contribute to increased gp120-CD4 interactions and Env fusogenicity...
  28. ncbi request reprint Transcriptional activity of blood-and cerebrospinal fluid-derived nef/long-terminal repeat sequences isolated from a slow progressor infected with nef-deleted human immunodeficiency virus type 1 (HIV-1) who developed HIV-associated dementia
    Melissa J Churchill
    The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    J Neurovirol 12:219-28. 2006
    ..Thus, nef and LTR mutations that augment transcription may contribute to neuropathogenesis of nef-deleted HIV-1...
  29. pmc CoRSeqV3-C: a novel HIV-1 subtype C specific V3 sequence based coreceptor usage prediction algorithm
    Kieran Cashin
    Center for Virology, Burnet Institute, 85 Commercial Rd, Melbourne 3004VIC, Australia
    Retrovirology 10:24. 2013
    ..In this study, we elucidated the V3 sequence determinants of C-HIV coreceptor usage, and used this knowledge to develop and validate a novel, user friendly, and highly sensitive C-HIV specific coreceptor usage prediction algorithm...
  30. pmc HIV-1 predisposed to acquiring resistance to maraviroc (MVC) and other CCR5 antagonists in vitro has an inherent, low-level ability to utilize MVC-bound CCR5 for entry
    Michael Roche
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    Retrovirology 8:89. 2011
    ..However, the CCR5-using CC1/85 strain appears to be uniquely predisposed to acquiring resistance to several CCR5 antagonists in vitro including MVC, vicriviroc and AD101...
  31. pmc Macrophage-tropic HIV-1 variants from brain demonstrate alterations in the way gp120 engages both CD4 and CCR5
    Hamid Salimi
    Center for Virology, Burnet Institute, Melbourne, Victoria, Australia
    J Leukoc Biol 93:113-26. 2013
    ..Our results suggest that BR-derived HIV-1 variants with highly efficient macrophage entry adopt conformations in gp120 that simultaneously alter the way in which the Env interacts with CD4 and CCR5...
  32. doi request reprint Both CD31(+) and CD31⁻ naive CD4(+) T cells are persistent HIV type 1-infected reservoirs in individuals receiving antiretroviral therapy
    Fiona Wightman
    Department of Medicine, Monash University, Melbourne, Australia
    J Infect Dis 202:1738-48. 2010
    ..Naive T cell recovery is critical for successful immune reconstitution after antiretroviral therapy (ART), but the relative contribution of CD31(+) and CD31⁻ naive T cells to immune reconstitution and viral persistence is unknown...
  33. ncbi request reprint Longitudinal analysis of nef/long terminal repeat-deleted HIV-1 in blood and cerebrospinal fluid of a long-term survivor who developed HIV-associated dementia
    Melissa Churchill
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Australia
    J Infect Dis 190:2181-6. 2004
    ..Thus, nef/LTR-deleted HIV-1 strains may undergo compartmentalized evolution in long-term survivors and cause neurologic disease...
  34. pmc Conformational alterations in the CD4 binding cavity of HIV-1 gp120 influencing gp120-CD4 interactions and fusogenicity of HIV-1 envelopes derived from brain and other tissues
    Lachlan Gray
    Center for Virology, Burnet Institute, Commercial Rd, Melbourne 3004, Australia
    Retrovirology 8:42. 2011
    ....
  35. pmc Reduced basal transcriptional activity of central nervous system-derived HIV type 1 long terminal repeats
    Lachlan R Gray
    Center for Virology, Burnet Institute, Melbourne, Victoria 3001, Australia
    AIDS Res Hum Retroviruses 29:365-70. 2013
    ..These findings have significance for understanding the molecular basis of HIV-1 persistence within cellular reservoirs of the CNS that need to be considered for strategies aimed at eradicating HIV-1...
  36. pmc Persistence of attenuated HIV-1 rev alleles in an epidemiologically linked cohort of long-term survivors infected with nef-deleted virus
    Melissa J Churchill
    The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Retrovirology 4:43. 2007
    ..Therefore, to determine whether defects in other viral genes contribute to attenuation of these HIV-1 strains, we characterized dominant HIV-1 rev alleles that persisted in 4 SBBC subjects; C18, C64, C98 and D36...
  37. ncbi request reprint Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues
    Melissa J Churchill
    The Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    J Neurovirol 12:146-52. 2006
    ..The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1...
  38. ncbi request reprint Impaired complement-mediated phagocytosis by HIV type-1-infected human monocyte-derived macrophages involves a cAMP-dependent mechanism
    Rula Azzam
    AIDS Pathogenesis and Clinical Research Program, Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Australia
    AIDS Res Hum Retroviruses 22:619-29. 2006
    ....
  39. doi request reprint Genetic and functional heterogeneity of CNS-derived tat alleles from patients with HIV-associated dementia
    Daniel Cowley
    Centre for Virology, Burnet Institute, 85 Commercial Rd, Melbourne, 3004 Victoria, Australia
    J Neurovirol 17:70-81. 2011
    ..Together, these data suggest that despite the observed heterogeneity in tat alleles isolated from matched lymphoid to CNS compartments, Tat function is maintained, highlighting the importance of Tat function in HIV-1 neuropathogenesis...
  40. pmc Longitudinal analysis of human immunodeficiency virus type 1 nef/long terminal repeat sequences in a cohort of long-term survivors infected from a single source
    Melissa J Churchill
    Macfarlane Burnet Institute for Medical Research and Public Health, GPO Box 2284, Melbourne 3001, Victoria, Australia
    J Virol 80:1047-52. 2006
    ..The in vivo pathogenicity of attenuated HIV-1 is therefore dictated by viral and/or host factors other than those that impose a unidirectional selection pressure on the nef/LTR region of the HIV-1 genome...
  41. pmc Frequency and Env determinants of HIV-1 subtype C strains from antiretroviral therapy-naive subjects that display incomplete inhibition by maraviroc
    Katharina Borm
    Center for Biomedical Research, Burnet Institute, Melbourne, Vic, Australia
    Retrovirology 13:74. 2016
    ....
  42. pmc Differences in coreceptor specificity contribute to alternative tropism of HIV-1 subtype C for CD4(+) T-cell subsets, including stem cell memory T-cells
    Kieran Cashin
    Center for Biomedical Research, Burnet Institute, Melbourne, 3004, Australia
    Retrovirology 11:97. 2014
    ....
  43. pmc Covariance of charged amino acids at positions 322 and 440 of HIV-1 Env contributes to coreceptor specificity of subtype B viruses, and can be used to improve the performance of V3 sequence-based coreceptor usage prediction algorithms
    Kieran Cashin
    Center for Biomedical Research, Burnet Institute, Melbourne, Australia Department of Microbiology and Immunology, University of Melbourne, Melbourne, Australia
    PLoS ONE 9:e109771. 2014
    ....
  44. ncbi request reprint Apoptosis induced in synchronized human immunodeficiency virus type 1-infected primary peripheral blood mononuclear cells is detected after the peak of CD4+ T-lymphocyte loss and is dependent on the tropism of the gp120 envelope glycoprotein
    Victoria A Lawson
    AIDS Cellular Biology Laboratory, Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Virology 327:70-82. 2004
    ....
  45. pmc HIV-1 entry and trans-infection of astrocytes involves CD81 vesicles
    Lachlan R Gray
    Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia Department of Biochemistry and Molecular Biology, Monash University, Victoria, Australia
    PLoS ONE 9:e90620. 2014
    ..The ability of astrocytes to transfer virus, without de novo viral synthesis suggests they are capable of sequestering and protecting virus and thus, they could potentially facilitate viral dissemination in the CNS. ..
  46. pmc Quantifying susceptibility of CD4+ stem memory T-cells to infection by laboratory adapted and clinical HIV-1 strains
    Jacqueline K Flynn
    Center for Biomedical Research, Burnet Institute, Melbourne, Victoria 3004, Australia
    Viruses 6:709-26. 2014
    ..Our system facilitates the quantitation of HIV-1 infection in alternative T cell subsets by CCR5- and CXCR4-using viruses across different HIV-1 subtypes, and will be useful for studies of HIV-1 pathogenesis and viral reservoirs. ..
  47. pmc The NRTIs lamivudine, stavudine and zidovudine have reduced HIV-1 inhibitory activity in astrocytes
    Lachlan R Gray
    Centre for Virology, Burnet Institute, Melbourne, Victoria, Australia
    PLoS ONE 8:e62196. 2013
    ..Our data suggest that 3TC, d4T and ZDV may not adequately target astrocyte infection in vivo, which has potential implications for their inclusion in Neuro-cART regimens...
  48. pmc A common mechanism of clinical HIV-1 resistance to the CCR5 antagonist maraviroc despite divergent resistance levels and lack of common gp120 resistance mutations
    Michael Roche
    Center for Virology, Monash University, Melbourne, Victoria, Australia
    Retrovirology 10:43. 2013
    ..To more precisely understand mechanisms of resistance to MVC in vivo, we characterized Envs isolated from 2 subjects who experienced virologic failure on MVC...
  49. pmc Genetic and functional analysis of R5X4 human immunodeficiency virus type 1 envelope glycoproteins derived from two individuals homozygous for the CCR5delta32 allele
    Lachlan Gray
    Macfarlane Burnet Institute for Medical Research and Public Health, GPO Box 2284, Melbourne 3001, Victoria, Australia
    J Virol 80:3684-91. 2006
    ..The persistence of CCR5-using HIV-1 in two CCR5delta32 homozygotes suggests the conserved CCR5 binding domain of Env is highly stable and provides new mechanistic insights important for HIV-1 transmission and persistence...
  50. ncbi request reprint The CD16+ monocyte subset is more permissive to infection and preferentially harbors HIV-1 in vivo
    Philip J Ellery
    AIDS Pathogenesis and Clinical Research Program, Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    J Immunol 178:6581-9. 2007
    ..Collectively, these data show that CD16+ monocytes are preferentially susceptible to HIV-1 entry, more permissive for replication, and constitute a continuing source of viral persistence during HAART...
  51. ncbi request reprint Astrocyte specific viral strains in HIV dementia
    Katherine A Thompson
    Neurovirology Research Laboratory, Macfarlane Burnet Institute for Medical Research and Public Health, Department of Medicine, Monash University, Melbourne, Victoria, Australia
    Ann Neurol 56:873-7. 2004
    ..These results demonstrate cell-specific compartmentalization of distinct R5-like viral strains in the central nervous system microenvironment...
  52. ncbi request reprint HIV-1 entry inhibitors: classes, applications and factors affecting potency
    Jasminka Sterjovski
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Curr HIV Res 4:387-400. 2006
    ....
  53. pmc A new way of measuring apoptosis by absolute quantitation of inter-nucleosomally fragmented genomic DNA
    David J Hooker
    Centre for Virology, Burnet Institute, 85 Commercial Road, Melbourne, Victoria 3004, Australia
    Nucleic Acids Res 40:e113. 2012
    ....
  54. doi request reprint Extremely prolonged HIV seroconversion associated with an MHC haplotype carrying disease susceptibility genes for antibody deficiency disorders
    Alex Padiglione
    Infectious Diseases Unit, Monash Medical Centre, Clayton, Victoria, Australia
    Clin Immunol 137:199-208. 2010
    ..This highlights the need for multiparameter testing, in particular use of a fourth generation HIV test, for confirming HIV infection and underscores the importance of host factors in HIV pathogenesis...
  55. pmc Measuring and monitoring apoptosis and drug toxicity in HIV patients by ligation-mediated polymerase chain reaction
    David J Hooker
    Centre for Virology, Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    J Cell Mol Med 13:948-58. 2009
    ..8 for each). Together these data demonstrate that LM-PCR is a robust and reliable quantifier of apoptosis with potential for basic science and clinical investigation...
  56. doi request reprint A novel, rapid method to detect infectious HIV-1 from plasma of persons infected with HIV-1
    Alyssa Cornall
    Infectious Diseases Unit, Alfred Hospital, Melbourne, Victoria, Australia
    J Virol Methods 165:90-6. 2010
    ..This technique could be used for rapid evaluation of viral fitness, co-receptor usage or drug resistance without the need for viral amplification...
  57. pmc Low TRBP levels support an innate human immunodeficiency virus type 1 resistance in astrocytes by enhancing the PKR antiviral response
    Chi L Ong
    Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria, Australia
    J Virol 79:12763-72. 2005
    ..Our findings highlight TRBP as a novel cellular target for therapeutic interventions to block productive HIV-1 replication in cells that are fully permissive for HIV-1 infection...
  58. pmc Virucidal activity of the dendrimer microbicide SPL7013 against HIV-1
    Sushama Telwatte
    Centres for Virology and Immunology, Burnet Institute, Melbourne, Victoria 3004, Australia
    Antiviral Res 90:195-9. 2011
    ..In addition, the mode of action by which SPL7013 prevents infection of cells with X4 and R5X4 strains is likely to differ from R5 strains of HIV-1...
  59. pmc Ex vivo response to histone deacetylase (HDAC) inhibitors of the HIV long terminal repeat (LTR) derived from HIV-infected patients on antiretroviral therapy
    Hao K Lu
    Department of Infectious Diseases, Monash University, Melbourne, Victoria, Australia Centre for Biomedical Research, Burnet Institute, Melbourne, Victoria, Australia
    PLoS ONE 9:e113341. 2014
    ..Together, our results suggest that the LTR sequence of integrated virus is not a major determinant of a functional response to an HDACi. ..
  60. pmc Is the central nervous system a reservoir of HIV-1?
    Lachlan R Gray
    aCentre for Biomedical Research, Burnet Institute bDepartment of Infectious Diseases, Monash University, Melbourne, Victoria cSouth Australian Health and Medical Research Institute, Adelaide, South Australia dDepartment of Microbiology and Immunology, University of Melbourne, Parkville eDepartment of Microbiology, Monash University, Clayton fDepartment of Medicine, Monash University, Melbourne, Victoria, Australia
    Curr Opin HIV AIDS 9:552-8. 2014
    ..To summarize the evidence in the literature that supports the central nervous system (CNS) as a viral reservoir for HIV-1 and to prioritize future research efforts...
  61. pmc Structure activity relationship of dendrimer microbicides with dual action antiviral activity
    David Tyssen
    Centres for Virology and Immunology, Burnet Institute, Melbourne, Victoria, Australia
    PLoS ONE 5:e12309. 2010
    ....
  62. pmc Reliable genotypic tropism tests for the major HIV-1 subtypes
    Kieran Cashin
    1 Center for Biomedical Research, Burnet Institute, Melbourne, Australia 3004 2 Department of Microbiology and Immunology, University of Melbourne, Parkville, Australia 3010
    Sci Rep 5:8543. 2015
    ..The PhenoSeq platform will inform the appropriate use of maraviroc and future CCR5 blocking drugs in regions of the world where non-B HIV-1 predominates, which are burdened the most by the HIV-1 pandemic. ..
  63. ncbi request reprint Isolation of human immunodeficiency virus type 1 from peripheral blood monocytes
    Paul R Gorry
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Methods Mol Biol 304:25-33. 2005
    ..The study of HIV-1 strains harbored by peripheral blood monocytes of patients undergoing HAART will contribute to the understanding of viral persistence in cellular reservoirs that impede effective HAART...
  64. ncbi request reprint Novel pathway of human immunodeficiency virus type 1 uptake and release in astrocytes
    Jennifer N Clarke
    Australian Centre for HIV and Hepatitis Virology Research, Infectious Diseases Laboratories, Institute of Medical and Veterinary Science, Adelaide, Australia
    Virology 348:141-55. 2006
    ..Our findings suggest a novel pathway of HIV-1 uptake and release in astrocytes that does not necessarily require virus replication, which may contribute to persistence and spread of HIV-1 in the brain...
  65. doi request reprint Transmission of HIV-1 drug-resistant variants: prevalence and effect on treatment outcome
    Martin R Jakobsen
    Department of Infectious Diseases, Aarhus University Hospital, Skejby, Denmark
    Clin Infect Dis 50:566-73. 2010
    ....
  66. doi request reprint Toxicity and in vitro activity of HIV-1 latency-reversing agents in primary CNS cells
    Lachlan R Gray
    Centre for Biomedical Research, Burnet Institute, 85 Commercial Rd, Melbourne, 3004, Vic, Australia
    J Neurovirol 22:455-63. 2016
    ..We recommend that future trials of LRA also examine the effects of these agents on the CNS via examination of cerebrospinal fluid. ..
  67. pmc Inhibition of catechol-O-methyl transferase (COMT) by tolcapone restores reductions in microtubule-associated protein 2 (MAP2) and synaptophysin (SYP) following exposure of neuronal cells to neurotropic HIV
    Ting Ting Lee
    Department of Psychiatry, The University of Melbourne, Level 1 North, Main Block, Royal Hospital, 3050, Melbourne, Vic, Australia
    J Neurovirol 21:535-43. 2015
    ..As HIV-associated dendritic and synaptic damage are contributors to HAND, inhibition of COMT may represent a potential strategy for attenuating or preventing some of the symptoms of HAND...
  68. pmc HIV-1 transcriptional regulation in the central nervous system and implications for HIV cure research
    Melissa J Churchill
    Center for Biomedical Research, Burnet Institute, 85 Commercial Rd, Melbourne, 3004, Victoria, Australia
    J Neurovirol 21:290-300. 2015
    ..A comprehensive understanding of viral transcription and latency in the CNS is required in order to determine treatment outcomes when using transcriptional activators within the CNS...
  69. ncbi request reprint Is there a role for plant-made vaccines in the prevention of HIV/AIDS?
    Diane E Webster
    Macfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia
    Immunol Cell Biol 83:239-47. 2005
    ....
  70. pmc Apoptosis of bystander T cells induced by human immunodeficiency virus type 1 with increased envelope/receptor affinity and coreceptor binding site exposure
    Geoffrey H Holm
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 78:4541-51. 2004
    ....
  71. pmc Increased CCR5 affinity and reduced CCR5/CD4 dependence of a neurovirulent primary human immunodeficiency virus type 1 isolate
    Paul R Gorry
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Virol 76:6277-92. 2002
    ....
  72. ncbi request reprint Regulation of CC chemokine receptor 5 and CD4 expression and human immunodeficiency virus type 1 replication in human macrophages and microglia by T helper type 2 cytokines
    Jianbin Wang
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA
    J Infect Dis 185:885-97. 2002
    ..These findings suggest that increased production of T helper type 2 cytokines in the later stages of disease can enhance virus entry and replication in mononuclear phagocytes and facilitate chemotactic migration...
  73. ncbi request reprint Persistence of dual-tropic HIV-1 in an individual homozygous for the CCR5 Delta 32 allele
    Paul R Gorry
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Lancet 359:1832-4. 2002
    ..We studied the persistence of dual-tropic HIV-1 in an individual homozygous for this deletion. Our results suggest that structural features of the HIV-1 envelope linked to CCR5 tropism could confer a selective advantage in vivo...
  74. pmc Genetic and functional analysis of full-length human immunodeficiency virus type 1 env genes derived from brain and blood of patients with AIDS
    Asa Ohagen
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts, USA
    J Virol 77:12336-45. 2003
    ....
  75. pmc Bioinformatic prediction programs underestimate the frequency of CXCR4 usage by R5X4 HIV type 1 in brain and other tissues
    Megan E Mefford
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, Massachusetts 02115, USA
    AIDS Res Hum Retroviruses 24:1215-20. 2008
    ..These results demonstrate that most predictive algorithms underestimate the frequency of R5X4 HIV-1 in brain and other tissues. SVM(geno2pheno) is the most accurate predictor of CXCR4 usage by R5X4 HIV-1...
  76. pmc The HIV Env variant N283 enhances macrophage tropism and is associated with brain infection and dementia
    Rebecca L Dunfee
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 103:15160-5. 2006
    ..001). These findings suggest that the macrophage-tropic HIV Env variant N283 is associated with brain infection and dementia in vivo, representing an example of a HIV variant associated with a specific AIDS-related complication...
  77. ncbi request reprint In vivo replication kinetics of a nef-deleted strain of HIV-1
    Suzanne M Crowe
    AIDS 19:842-3. 2005
  78. doi request reprint Primary HIV-1 R5 isolates from end-stage disease display enhanced viral fitness in parallel with increased gp120 net charge
    Johanna Repits
    Department of Laboratory Medicine, Lund University, Sweden
    Virology 379:125-34. 2008
    ..In summary, this study suggests that R5 HIV-1 variants with increased gp120 net charge emerge in an opportunistic manner during severe immunodeficiency. Thus, we here propose a new mechanism by which HIV-1 may gain fitness...
  79. pmc Changes in the V3 region of gp120 contribute to unusually broad coreceptor usage of an HIV-1 isolate from a CCR5 Delta32 heterozygote
    Paul R Gorry
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Virology 362:163-78. 2007
    ....
  80. ncbi request reprint CXCR4 or CCR5 tropism of human immunodeficiency virus type 1 isolates does not determine the immunological milieu in patients responding to antiretroviral therapy
    Patricia Price
    School of Surgery and Pathology, University of Western Australia, Perth, WA, Australia
    Viral Immunol 19:734-40. 2006
    ..Levels of LAG-3 (lymphocyte activation gene-3 protein) were elevated in patients with uncontrolled HIV replication. Hence the immunological milieu did not reflect HIV tropism...
  81. ncbi request reprint Mechanisms of HIV-1 neurotropism
    Rebecca Dunfee
    Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA
    Curr HIV Res 4:267-78. 2006
    ..Understanding mechanisms that underlie HIV neurotropism and neurovirulence is critical for development of therapeutics to inhibit CNS infection and preventing neurological injury in HIV-infected individuals...
  82. ncbi request reprint Highly active antiretroviral therapy and human immunodeficiency virus encephalitis
    Jennifer E Cook
    Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA
    Ann Neurol 57:795-803. 2005
    ..HAART significantly decreased the amount of astrogliosis and viral load in treated mice compared with mice that received vehicle injections. These studies offer insight into the ability of HAART to treat HIV infection of the brain...

Research Grants1

  1. Pathogenesis of Macrophage Tropic HIV-1
    Paul Gorry; Fiscal Year: 2004
    ..Understanding the molecular basis of enhanced M-tropism will provide further insights into HIV-1 pathogenesis, which is vital for the development of new inhibitors for the treatment of HIV-1 infection. ..