Michael T Ryan

Summary

Affiliation: La Trobe University
Country: Australia

Publications

  1. ncbi Mitochondrial-nuclear communications
    Michael T Ryan
    Department of Biochemistry, La Trobe University, Melbourne 3086, Australia
    Annu Rev Biochem 76:701-22. 2007
  2. ncbi Mitochondrial protein-import machinery: correlating structure with function
    Michael J Baker
    Department of Biochemistry, La Trobe University, Melbourne 3086, Australia
    Trends Cell Biol 17:456-64. 2007
  3. ncbi Chaperones: inserting beta barrels into membranes
    Michael T Ryan
    Department of Biochemistry, La Trobe University 3088, Melbourne, Australia
    Curr Biol 14:R207-9. 2004
  4. ncbi Dissection of the mitochondrial import and assembly pathway for human Tom40
    Adam D Humphries
    Department of Biochemistry, La Trobe University, Melbourne 3086, Australia
    J Biol Chem 280:11535-43. 2005
  5. pmc Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import
    Michael J Baker
    Department of Biochemistry, La Trobe University, Melbourne 3086, Victoria, Australia
    Mol Biol Cell 20:769-79. 2009
  6. ncbi Import of nuclear-encoded proteins into mitochondria
    Diana Stojanovski
    Department of Biochemistry, La Trobe University 3086, Melbourne, Australia
    Exp Physiol 88:57-64. 2003
  7. pmc MiD49 and MiD51, new components of the mitochondrial fission machinery
    Catherine S Palmer
    La Trobe Institute for Molecular Science, La Trobe University, Plenty Road, Melbourne 3086, Victoria, Australia
    EMBO Rep 12:565-73. 2011
  8. doi Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria
    Michael Lazarou
    Department of Biochemistry, La Trobe University, Melbourne, Australia
    FEBS J 276:6701-13. 2009
  9. doi Impaired folding of the mitochondrial small TIM chaperones induces clearance by the i-AAA protease
    Michael J Baker
    Department of Biochemistry, La Trobe Institute for Molecular Science and ARC Centre of Excellence for Coherent X ray Science, La Trobe University, Melbourne 3086, Australia
    J Mol Biol 424:227-39. 2012
  10. doi Next-generation sequencing in molecular diagnosis: NUBPL mutations highlight the challenges of variant detection and interpretation
    Elena J Tucker
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, Victoria, Australia
    Hum Mutat 33:411-8. 2012

Collaborators

Detail Information

Publications53

  1. ncbi Mitochondrial-nuclear communications
    Michael T Ryan
    Department of Biochemistry, La Trobe University, Melbourne 3086, Australia
    Annu Rev Biochem 76:701-22. 2007
    ..This review covers the mechanisms of mitochondrial biogenesis and the way cells respond to external signals to maintain mitochondrial function and cellular homeostasis...
  2. ncbi Mitochondrial protein-import machinery: correlating structure with function
    Michael J Baker
    Department of Biochemistry, La Trobe University, Melbourne 3086, Australia
    Trends Cell Biol 17:456-64. 2007
    ..Future challenges for the cell biologists will be to use this structural information to test specific hypotheses addressing the molecular mechanisms of mitochondrial protein import...
  3. ncbi Chaperones: inserting beta barrels into membranes
    Michael T Ryan
    Department of Biochemistry, La Trobe University 3088, Melbourne, Australia
    Curr Biol 14:R207-9. 2004
    ..New work has identified a sorting and assembly machinery that chaperones beta-barrels into the mitochondrial outer membrane and is evolutionarily conserved from bacteria to man...
  4. ncbi Dissection of the mitochondrial import and assembly pathway for human Tom40
    Adam D Humphries
    Department of Biochemistry, La Trobe University, Melbourne 3086, Australia
    J Biol Chem 280:11535-43. 2005
    ..Nevertheless, we show that Tom40 assembly is reduced in mitochondria depleted of human Sam50. These findings are discussed in context with current models from fungal studies...
  5. pmc Structural and functional requirements for activity of the Tim9-Tim10 complex in mitochondrial protein import
    Michael J Baker
    Department of Biochemistry, La Trobe University, Melbourne 3086, Victoria, Australia
    Mol Biol Cell 20:769-79. 2009
    ..We conclude that Tim9 plays an important functional role that includes facilitating the initial steps in translocating precursor substrates into the intermembrane space...
  6. ncbi Import of nuclear-encoded proteins into mitochondria
    Diana Stojanovski
    Department of Biochemistry, La Trobe University 3086, Melbourne, Australia
    Exp Physiol 88:57-64. 2003
    ..We also outline the various targeting pathways that preproteins can take in order to reach their appropriate sub-mitochondrial compartment...
  7. pmc MiD49 and MiD51, new components of the mitochondrial fission machinery
    Catherine S Palmer
    La Trobe Institute for Molecular Science, La Trobe University, Plenty Road, Melbourne 3086, Victoria, Australia
    EMBO Rep 12:565-73. 2011
    ..Overexpression of MiD49/51 seems to sequester Drp1 from functioning at mitochondria and cause fused tubules to associate with actin. Thus, MiD49/51 are new mediators of mitochondrial division affecting Drp1 action at mitochondria...
  8. doi Assembly of nuclear DNA-encoded subunits into mitochondrial complex IV, and their preferential integration into supercomplex forms in patient mitochondria
    Michael Lazarou
    Department of Biochemistry, La Trobe University, Melbourne, Australia
    FEBS J 276:6701-13. 2009
    ..We conclude that newly imported nuclear DNA-encoded subunits can integrate into the complex IV holoenzyme and supercomplex forms by associating with pre-existing subunits and intermediate assembly complexes...
  9. doi Impaired folding of the mitochondrial small TIM chaperones induces clearance by the i-AAA protease
    Michael J Baker
    Department of Biochemistry, La Trobe Institute for Molecular Science and ARC Centre of Excellence for Coherent X ray Science, La Trobe University, Melbourne 3086, Australia
    J Mol Biol 424:227-39. 2012
    ..We delineate a clearance mechanism for the mutant proteins and their unassembled wild-type partner protein by the mitochondrial ATP-dependent protease, Yme1 (yeast mitochondrial escape 1)...
  10. doi Next-generation sequencing in molecular diagnosis: NUBPL mutations highlight the challenges of variant detection and interpretation
    Elena J Tucker
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, Victoria, Australia
    Hum Mutat 33:411-8. 2012
    ..It is important that locus-specific databases contain accurate information on pathogenic variation. NGS data, therefore, require rigorous experimental follow-up to confirm mutation pathogenicity...
  11. pmc Analysis of the assembly profiles for mitochondrial- and nuclear-DNA-encoded subunits into complex I
    Michael Lazarou
    Department of Biochemistry, La Trobe University, Melbourne, Australia
    Mol Cell Biol 27:4228-37. 2007
    ..Subunit exchange may also act as an efficient mechanism to prevent the accumulation of oxidatively damaged subunits that would otherwise be detrimental to mitochondrial oxidative phosphorylation and have the potential to cause disease...
  12. pmc Structural and functional analysis of MiD51, a dynamin receptor required for mitochondrial fission
    Viviane Richter
    Department of Biochemistry and 2 Australian Research Council Centre of Excellence in Coherent X Ray Science, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia
    J Cell Biol 204:477-86. 2014
    ..MiD51 foci are also dependent on the presence of Drp1, and after scission they are distributed to daughter organelles, supporting the involvement of MiD51 in the fission apparatus. ..
  13. pmc A founder mutation in PET100 causes isolated complex IV deficiency in Lebanese individuals with Leigh syndrome
    Sze Chern Lim
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, VIC 3052, Australia Department of Paediatrics, University of Melbourne, Melbourne, VIC 3052, Australia
    Am J Hum Genet 94:209-22. 2014
    ..The mutation was estimated to have arisen at least 520 years ago, explaining how the families could have different religions and different geographic origins within Lebanon...
  14. doi Understanding mitochondrial complex I assembly in health and disease
    Masakazu Mimaki
    Department of Biochemistry, La Trobe University, Melbourne, VIC 3086, Australia
    Biochim Biophys Acta 1817:851-62. 2012
    ..This article is part of a Special Issue entitled: Biogenesis/Assembly of Respiratory Enzyme Complexes...
  15. doi Assembly of mitochondrial complex I and defects in disease
    Michael Lazarou
    Department of Biochemistry, La Trobe University, 3086 Melbourne, Australia
    Biochim Biophys Acta 1793:78-88. 2009
    ..Here we review how complex I is assembled and the factors required to mediate this process...
  16. ncbi Analysis of mitochondrial subunit assembly into respiratory chain complexes using Blue Native polyacrylamide gel electrophoresis
    Matthew McKenzie
    Department of Biochemistry, La Trobe University, Melbourne, VIC 3086, Australia
    Anal Biochem 364:128-37. 2007
    ..Furthermore, we show that this approach is highly suited for the rapid detection of respiratory complex assembly defects in fibroblasts from patients with mitochondrial disease and, thus, has potential diagnostic applications...
  17. doi Assembly factors of human mitochondrial complex I and their defects in disease
    Matthew McKenzie
    Department of Biochemistry, La Trobe University, Melbourne, Vic, Australia
    IUBMB Life 62:497-502. 2010
    ..Defects in assembly factors which disrupt complex I assembly and contribute to human disease pathogenesis will also be discussed...
  18. ncbi Levels of human Fis1 at the mitochondrial outer membrane regulate mitochondrial morphology
    Diana Stojanovski
    Department of Biochemistry, La Trobe University, 3086 Melbourne, Australia
    J Cell Sci 117:1201-10. 2004
    ..These results indicate that the levels of hFis1 at the mitochondrial surface influences mitochondrial fission events and hence overall mitochondrial morphology within the cell...
  19. pmc A mitochondrial specific stress response in mammalian cells
    Quan Zhao
    Department of Biochemistry, La Trobe University, Melbourne, Victoria 3086, Australia
    EMBO J 21:4411-9. 2002
    ....
  20. doi The regulation of mitochondrial morphology: intricate mechanisms and dynamic machinery
    Catherine S Palmer
    La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia
    Cell Signal 23:1534-45. 2011
    ..This review focuses on our current understanding of the mitochondrial morphology machinery in cell homeostasis, apoptosis and neurodegeneration, and the post translational modifications that regulate these processes...
  21. pmc Inhibition of Bak activation by VDAC2 is dependent on the Bak transmembrane anchor
    Michael Lazarou
    La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia
    J Biol Chem 285:36876-83. 2010
    ....
  22. doi Chapter 18 Analysis of respiratory chain complex assembly with radiolabeled nuclear- and mitochondrial-encoded subunits
    Matthew McKenzie
    Department of Biochemistry, La Trobe University, Melbourne, Australia
    Methods Enzymol 456:321-39. 2009
    ..Here we describe methods to monitor the assembly of newly synthesized subunits encoded by mitochondrial DNA from cultured mammalian cells, as well as the import and assembly of individual subunits encoded by nuclear DNA...
  23. doi Human Miltons associate with mitochondria and induce microtubule-dependent remodeling of mitochondrial networks
    Olga S Koutsopoulos
    Department of Biochemistry, La Trobe University, 3086 Melbourne, Australia
    Biochim Biophys Acta 1803:564-74. 2010
    ..We conclude that the human Miltons are important mediators of the mitochondrial trafficking machinery...
  24. doi Characterization of mitochondrial FOXRED1 in the assembly of respiratory chain complex I
    Luke E Formosa
    Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC 3086, Australia, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Melbourne 3800, Australia
    Hum Mol Genet 24:2952-65. 2015
    ..Our studies reveal that FOXRED1 is a crucial component in the productive assembly of complex I and that mutations in FOXRED1 leading to partial loss of function cause defects in complex I biogenesis. ..
  25. pmc Mutations in MTFMT underlie a human disorder of formylation causing impaired mitochondrial translation
    Elena J Tucker
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, VIC 3052, Australia
    Cell Metab 14:428-34. 2011
    ..Our findings demonstrate that MTFMT is critical for efficient human mitochondrial translation and reveal a human disorder of Met-tRNA(Met) formylation...
  26. doi Accessory subunits are integral for assembly and function of human mitochondrial complex I
    David A Stroud
    Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, 3800, Melbourne, Australia
    Nature 538:123-126. 2016
    ..Coupling gene-editing technology with proteomics represents a powerful tool for dissecting large multi-subunit complexes and enables the study of complex dysfunction at a cellular level...
  27. ncbi Import and assembly of proteins into mitochondria of mammalian cells
    Nicholas J Hoogenraad
    Department of Biochemistry, La Trobe University, Melbourne 3086, Australia
    Biochim Biophys Acta 1592:97-105. 2002
    ..We also discuss diseases associated with defects in import and assembly of mitochondrial proteins and what is currently known about the regulation of import in mammals...
  28. doi Splitting up the powerhouse: structural insights into the mechanism of mitochondrial fission
    Viviane Richter
    La Trobe Institute for Molecular Sciences, La Trobe University, Melbourne, 3086, Australia
    Cell Mol Life Sci 72:3695-707. 2015
    ..This review focuses on our current understanding of mitochondrial fission and the proteins that regulate this process in cell homeostasis, with a particular focus on the recent mechanistic insights based on protein structures. ..
  29. ncbi Insertion and assembly of human tom7 into the preprotein translocase complex of the outer mitochondrial membrane
    Amelia J Johnston
    Department of Biochemistry, La Trobe University, 3086 Melbourne, Australia
    J Biol Chem 277:42197-204. 2002
    ..These results indicate that the levels of Tom22 within mitochondria dictate the assembly of TOM complexes and hence may regulate its biogenesis...
  30. doi Recent advances into the understanding of mitochondrial fission
    Kirstin Elgass
    Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Australia
    Biochim Biophys Acta 1833:150-61. 2013
    ..This article is part of a Special Issue entitled: Mitochondrial dynamics and physiology...
  31. pmc Gene knockout using transcription activator-like effector nucleases (TALENs) reveals that human NDUFA9 protein is essential for stabilizing the junction between membrane and matrix arms of complex I
    David A Stroud
    Department of Biochemistry, La Trobe Institute for Molecular Science, and ARC Centre of Excellence for Coherent X ray Science, La Trobe University, Melbourne 3086, Australia
    J Biol Chem 288:1685-90. 2013
    ..We conclude that NDUFA9 is involved in stabilizing the junction between membrane and matrix arms of complex I, a late assembly step critical for complex I biogenesis and activity...
  32. pmc Mutation of C20orf7 disrupts complex I assembly and causes lethal neonatal mitochondrial disease
    Canny Sugiana
    Mitochondrial and Metabolic Research Group, Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, VIC 3052, Australia
    Am J Hum Genet 83:468-78. 2008
    ..Our results indicate that C20orf7 is crucial in the assembly of complex I and that mutations in C20orf7 cause mitochondrial disease...
  33. ncbi Mitochondrial morphology and distribution in mammalian cells
    Ann E Frazier
    Department of Biochemistry, La Trobe University, 3086 Melbourne, Australia
    Biol Chem 387:1551-8. 2006
    ..Here we review our current understanding of the functions of the main players involved in mitochondrial fission, fusion and distribution in mammalian cells...
  34. doi A novel isoform of the human mitochondrial complex I subunit NDUFV3
    Marris G Dibley
    Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Australia
    FEBS Lett . 2016
    ..While the 50 kDa isoform appears to be dominant in HEK293T cells, we find either isoform alone is sufficient for assembly of mature complex I. NDUFV3 represents the first known complex I subunit present in two functional isoforms...
  35. doi COA6 is a mitochondrial complex IV assembly factor critical for biogenesis of mtDNA-encoded COX2
    David A Stroud
    Department of Biochemistry and Molecular Biology, Monash University, Clayton 3800, Melbourne, Australia
    Hum Mol Genet 24:5404-15. 2015
    ..Our data reveal that COA6 is intricately involved in the copper-dependent biogenesis of COX2. ..
  36. doi Mitochondria: organization of respiratory chain complexes becomes cristae-lized
    David A Stroud
    Department of Biochemistry, La Trobe Institute for Molecular Science, La Trobe University, Melbourne 3086, Australia
    Curr Biol 23:R969-71. 2013
    ..New research shows that these two characteristics are more dependent on each other than previously thought. ..
  37. pmc Mutations in the UQCC1-interacting protein, UQCC2, cause human complex III deficiency associated with perturbed cytochrome b protein expression
    Elena J Tucker
    Murdoch Children s Research Institute, Royal Children s Hospital, Parkville, Victoria, Australia Department of Paediatrics, University of Melbourne, Melbourne, Victoria, Australia
    PLoS Genet 9:e1004034. 2013
    ....
  38. ncbi Mitochondrial respiratory chain supercomplexes are destabilized in Barth Syndrome patients
    Matthew McKenzie
    Department of Biochemistry, La Trobe University, Melbourne, Australia
    J Mol Biol 361:462-9. 2006
    ..We propose that the loss of mature cardiolipin species in Barth Syndrome results in unstable respiratory chain supercomplexes, thereby affecting Complex I biogenesis, respiratory activities and subsequent pathology...
  39. pmc NDUFS6 mutations are a novel cause of lethal neonatal mitochondrial complex I deficiency
    Denise M Kirby
    Murdoch Childrens Research Institute, Royal Children s Hospital, Melbourne, Victoria, Australia
    J Clin Invest 114:837-45. 2004
    ..This integrated approach to gene identification offers promise for identifying other unknown causes of respiratory chain disorders...
  40. pmc Mitochondrial fusion: Reaching the end of mitofusin's tether
    Luke E Formosa
    Department of Biochemistry and Molecular Biology, Monash Biomedicine Discovery Institute, Monash University, Melbourne, Victoria 3800, Australia
    J Cell Biol 215:597-598. 2016
    ..This work proposes a new model to explain the important and elusive process of MFN-mediated mitochondrial fusion...
  41. doi Translation and Assembly of Radiolabeled Mitochondrial DNA-Encoded Protein Subunits from Cultured Cells and Isolated Mitochondria
    Luke E Formosa
    Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Vic, 3086, Australia
    Methods Mol Biol 1351:115-29. 2016
    ....
  42. pmc Mapping biological composition through quantitative phase and absorption X-ray ptychography
    Michael W M Jones
    ARC Centre of Excellence for Coherent X ray Science, Department of Physics, La Trobe University, Bundoora 3086, Australia
    Sci Rep 4:6796. 2014
    ..While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered. ..
  43. pmc Mdm38 interacts with ribosomes and is a component of the mitochondrial protein export machinery
    Ann E Frazier
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, D 79104 Freiburg, Germany
    J Cell Biol 172:553-64. 2006
    ....
  44. pmc Mitochondrial protein import: precursor oxidation in a ternary complex with disulfide carrier and sulfhydryl oxidase
    Diana Stojanovski
    Institut fur Biochemie und Molekularbiologie, Zentrum für Biochemie und Molekulare Zellforschung, Universitat Freiburg, 79104 Freiburg, Germany
    J Cell Biol 183:195-202. 2008
    ....
  45. ncbi Biogenesis of yeast mitochondrial cytochrome c: a unique relationship to the TOM machinery
    Nils Wiedemann
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Strasse 7, Germany
    J Mol Biol 327:465-74. 2003
    ..We conclude that up to two components of the TOM complex, Tom22 and possibly the GIP, are involved in the biogenesis of cytochrome c...
  46. ncbi Machinery for protein sorting and assembly in the mitochondrial outer membrane
    Nils Wiedemann
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Hermann Herder Str 7, D 79104 Freiburg, Germany
    Nature 424:565-71. 2003
    ....
  47. pmc Essential role of Isd11 in mitochondrial iron-sulfur cluster synthesis on Isu scaffold proteins
    Nils Wiedemann
    Institut fur Biochemie und Molekularbiologie, Universitat Freiburg, Freiburg, Germany
    EMBO J 25:184-95. 2006
    ..We conclude that Isd11 is an indispensable eukaryotic component of the mitochondrial machinery for biogenesis of Fe/S proteins...
  48. ncbi Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70
    Stewart D Nuttall
    CSIRO Health Sciences and Nutrition, Parkville, Victoria, Australia
    Eur J Biochem 270:3543-54. 2003
    ....
  49. ncbi Low mutant load of mitochondrial DNA G13513A mutation can cause Leigh's disease
    Denise M Kirby
    Murdoch Children s Research Institute, University of Melbourne, Victoria, Australia
    Ann Neurol 54:473-8. 2003
    ..We conclude that the G13513A mutation causes a complex I defect when present at unusually low mutant load and may act dominantly...
  50. ncbi De novo mutations in the mitochondrial ND3 gene as a cause of infantile mitochondrial encephalopathy and complex I deficiency
    Robert McFarland
    Mitochondrial Research Group, School of Neurology, Neurobiology and Psychiatry, University of Newcastle upon Tyne, United Kingdom
    Ann Neurol 55:58-64. 2004
    ..Mitochondrial DNA disease may be considerably more prevalent in the pediatric population than currently predicted and should be considered in patients with infantile mitochondrial encephalopathies and complex I deficiency...
  51. ncbi Characterization of presenilin complexes from mouse and human brain using Blue Native gel electrophoresis reveals high expression in embryonic brain and minimal change in complex mobility with pathogenic presenilin mutations
    Janetta G Culvenor
    Department of Pathology, The University of Melbourne, Australia
    Eur J Biochem 271:375-85. 2004
    ..Higher molecular mass presenilin 1-reactive species were detected in brain containing presenilin 1 exon 9 deletion mutation. This abnormality was confirmed using cells transfected with the same presenilin deletion mutation...
  52. ncbi Biochemical and molecular diagnosis of mitochondrial respiratory chain disorders
    David R Thorburn
    Murdoch Children s Research Institute, Royal Children s Hospital, Melbourne, Victoria 3052 Australia
    Biochim Biophys Acta 1659:121-8. 2004
    ..A number of approaches offer promise to guide the choice of candidate genes, including Blue Native-PAGE immunoblotting and microarray expression analysis...
  53. ncbi Crystal structure of the mitochondrial chaperone TIM9.10 reveals a six-bladed alpha-propeller
    Chaille T Webb
    Structural Biology Division, The Walter and Eliza Hall Institute of Medical Research, 1G Royal Parade, Parkville, Victoria 3050, Australia
    Mol Cell 21:123-33. 2006
    ..10 as surrogate lipid contacts. The first reported crystal structure of a mitochondrial translocase assembly provides insights into selectivity and regulation of precursor import...