capsid

..host protein, p32, which is the receptor of complement 1 protein's (C1q) "globular head" as rubella virus/vaccine capsid protein binder. Perturbations in p32 functions have been implicated in SLE-associated arthritis...

..The immunogenicity of these polysaccharides is enhanced by their binding to carrier proteins. Viral surface capsid proteins are commonly found to be protective antigens and suitable as vaccine candidate. SALMONELLA TYPHI...

..C) Our laboratory has also been investigating the role of the HIV-1 capsid protein (CA) in early postentry events, a stage in the infectious process that is still not completely understood...

..Our research focuses on the large-scale conformational changes that accompany capsid maturation and on the interaction of viruses with host cells. We are currently pursuing five subprojects...

..HIV-1 isolates and that the compound acts by disrupting a late step in Gag processing involving conversion of the capsid precursor (p25) to mature capsid protein (p24)...

..All enteroviruses have a similar RNA genome of approximately 7.5 kb that encodes four capsid proteins and a number of nonstructural proteins...

..The need to manipulate AAV capsids for specific tissue delivery has generated interest in understanding their capsid structures. Previously we have reported the structure of the AAV5 capsid (Walters et al...

..We have made the unexpected discovery that one of the virion proteins, the capsid protein (C), is involved in an early step in virus RNA synthesis...

..We will investigate the process by which these viruses utilize their capsid protein for genome RNA recruitment, assembly, and association with the transmembrane glycoproteins...

..examine the interactions of the viral RNAs with the RNA-dependent RNA polymerase during replication and with the capsid protein precursor during virus assembly...

..While there are crystal structures for several of the major Ad capsid proteins (hexon, penton base and fiber), we lack detailed knowledge of their association upon assembly into intact ..

..Other DNA viruses interact with ND10s early after infections, but these interactions, which do not involve capsid proteins, lead to disruption of the structures...

..hepadnaviruses, for example conversion of RNA to a DNA genome during assembly and requirement for only a single capsid protein...

..hepadnaviruses, for example, conversion of RNA to a DNA genome during assembly and requirement for only a single capsid protein...

..While there are crystal structures for several of the major Ad capsid proteins (hexon, penton base and fiber), we lack detailed knowledge of their association upon assembly into intact ..

..In this regard we recently demonstrated that we could engineer phage by changing a single amino acid in a major capsid protein such that the resulting phage can remain in the mammalian circulatory system with an efficacy of more than ..

..In the case of other successful vaccines, such as HBsAg for hepatitis B virus, and L1 capsid protein of human papilloma virus, assembled particles were up to 1,000-fold more potent than the same weight of ..

..are to 1) determine the atomic structure of papilloma viruses in order to understand molecular mechanisms in capsid assembly and disassembly (uncoating), and 2) understand the structural basis of papilloma virus surface ..

..Importantly, 6 different AAV serotypes have been isolated (AAV 1-6) which differ in their respective capsid proteins...

..Atomic resolution structures of human papillomavirus (HPV) subtypes-11 and 5 L1 capsid proteins, HPV L1+L2 capsid protein complexes, and bovine papillomavirus virions will be determined...

..goal of this application is to characterize the protein dynamics of Hepatitis B virus (HBV) assembly, both in the capsid protein dimer and the assembled capsid of 120 HBV dimers, with the eye towards providing a new anti-viral ..

..goal of this application is to characterize the protein dynamics of Hepatitis B virus (HBV) assembly, both in the capsid protein dimer and the assembled capsid of 120 HBV dimers, with the eye towards providing a new anti-viral ..

..5% identical in DNA sequence, yet which differ in host range through mutations in the capsid protein which affect specific receptor binding...

..We will build on our previous cryoEM and crystallographic studies of the particle and capsid protein by performing in vitro analysis of particle degradation with cesium sulfate and doing crystallography of ..

..This structure, while revealing a capsid shell almost identical to those of other herpesviruses, nevertheless exhibited striking differences at the ..

..We will build on our previous cryoEM and crystallographic studies of the particle and capsid protein by performing in vitro analysis of particle degradation with cesium sulfate and doing crystallography of ..

..significant insight has come from studies of the immune response to virus-like-particles generated when NoV capsid proteins are over expressed...

..In addition to a VPg-linked genome, mature virion contains the major capsid proteins VP2 and VP3, the viral polymerase VP1, and possibly the viral protease VP4...

..In addition to a VPg-linked genome, mature virion contains the major capsid proteins VP2 and VP3, the viral polymerase VP1, and possibly the viral protease VP4...

..to be different, with a unique mutation near the active site of 2a protease that may contribute to the altered capsid proteins previously demonstrated in the variant virus...

..HPV virions consist of a molecule of double-stranded circular DNA contained within an icosahedral capsid composed of L1 major capsid protein and L2 minor capsid protein...

..HPV virions consist of a molecule of double-stranded circular DNA contained within an icosahedral capsid composed of L1 major capsid protein and L2 minor capsid protein...

..that can be infected with B19, and show that there appears to be multiple blocks, including at viral entry and capsid protein trafficking...

..Using recombinant SV40 capsid proteins, it is possible to package DNA and RNA in vitro...

..We will continue our studies of how the proteins of the capsid interact and why, and what modifications they undergo and how these govern the process of capsid formation and ..

..Papillomavirus has only two capsid proteins; L1 and L2...

..The virion outer capsid protein, VP5, which appears to have fusigenic activity and facilitate virus entry into the cytoplasm is key to ..

DESCRIPTION (provided by applicant): The retroviral CA (or capsid) protein has several essential roles in the virus life cycle organizing the packing of the Gag polyprotein during immature particle assembly, directing morphogenesis of the ..

..Papillomavirus has only two capsid proteins; L1 and L2...

..MG selective transduction profiles can be selected for using replication-competent AAV combinatorial capsid protein libraries characterized by randomized virion surface loops; (2) AAV vectors that display dominant T-cell ..

..conformational changes in the virion, and the viral usurping of normal cellular functions via a number of non-capsid proteins...

..of virus to the tegument will be investigated by the immunofluorescence co-localization of VSVG and retroviral capsid proteins, and ultrastructural techniques...

..virion is composed of a glycoprotein-containing envelope, a tegument layer, and an icosahedral, bacteriophage-like capsid enclosing a double-stranded DNA genome...

..of virus to the tegument will be investigated by the immunofluorescence co-localization of VSVG and retroviral capsid proteins, and ultrastructural techniques...

..virion is composed of a glycoprotein-containing envelope, a tegument layer, and an icosahedral, bacteriophage-like capsid enclosing a double-stranded DNA genome...

..The two HPV capsid proteins, L1 and L2, are both independent protective antigens...

DESCRIPTION (provided by applicant): Capsid proteins of papillomaviruses (PV) have evolved functional domains for the proper interaction between themselves, with PV viral nonstructural proteins, with PV genomic DNA, and with host cell ..

DESCRIPTION (provided by applicant): Capsid proteins of papillomaviruses (PV) have evolved functional domains for the proper interaction between themselves, with PV viral nonstructural proteins, with PV genomic DNA, and with host cell ..

DESCRIPTION (provided by applicant): Like all herpesviruses, HSV-1 consists of an icosahedral capsid surrounded by a membrane envelope. The capsid, which contains the virus DNA, is assembled in the infected cell nucleus...

..critical - distinction between pathogenic and non-pathogenic infections is that antibody responses to the Gag p27 capsid protein, but not to other viral proteins, are weak to undetectable in the setting of nonpathogenic infections...

..caveat we have a novel strategy, termed shielding, in which we have technology to genetically modify the virion capsid to provide an uniformly shielded Ad vector...

..the infectious virion is composed of a glycoprotein containing envelope, a tegument layer, and an icosahedral capsid enclosing a dsDNA genome...

..a motif that is important for virus assembly and located at the C-terminal region of murine leukemia virus (MLV) capsid (CA)...

..The central hypothesis of this proposal is that by characterizing and modifying the AAV capsid proteins we will not only increase our understanding of viral tissue tropism, entry, immunoreactivity, and ..

..The central hypothesis of this proposal is that by characterizing and modifying the AAV capsid proteins we will not only increase our understanding of viral tissue tropism, entry, immunoreactivity, and ..

..rAAV has a simple structure consisting of 3 capsid proteins and a single-stranded DNA genome...

..with a diameter of about 2000 A and consists of several compartments: glycoproteins, membrane, tegument layer, capsid and dsDNA...

..to HPV disease are unknown, but previous studies have focused on HPV genotype-specific response against the major capsid protein, LI...

..Current research focuses on identifying functional domains of the viral capsid that permit efficient infection of specific cell types in order to advance the design of ideal virions for gene ..

HOC and SOC are dispensable T4 capsid proteins that can be used for phage display of multiple copies of peptides and proteins...

..These results are interesting in the context of RNA-protein interaction, as well as for the discovery of antiNC agents for AIDS therapy...

..This complex could become an important target for anti-HIV drug development, where a drug could exert its action near a high-energy intermediate on the pathway for maturation of the dimer...

..the idea that the HSV-1 UL6 gene product (pUL6) forms the portal through which viral DNA passes as it enters the capsid. Since DNA must enter at a unique site, immunoelectron microscopy experiments were undertaken to determine the ..

..5 promotes the formation of the icosahedral capsid shell through its association with the major capsid protein VP5 and through intermolecular interactions with ..

..It has been proposed that packaging is initiated by interaction of genome-bound Rep proteins to the capsid, thereby targeting the genome to the portal of encapsidation...

Rotavirus is a major pathogen of infantile gastroenteritis. It is a large and complex virus with a multilayered capsid organization that integrates the determinants of host specificity, cell entry, and the enzymatic functions necessary ..

..This process is critically dependent on the spacer p2, located between the capsid and the nucleocapsid proteins...

..These findings show the variation between predicted and actual transcription and indicate the complex nature of transcription from HCMV ORFs...

..The first structure reveals a complex assembly in the interior of the capsid, which involves the scaffolding, and the core complex, which plays an important role in DNA packaging and is ..

..This result suggests that the zinc-finger motif is not a required aspect of coat protein activation of replication in ilarviruses...

Human polyomavirus is a naked capsid virus containing a closed circular double-stranded DNA genome. The mechanism of DNA encapsidation for the viral progeny formation is not fully understood...

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Rotavirus has a complex triple-layered icosahedral capsid. The external layer consists of VP7 and VP4, the intermediate layer consists of VP6 trimers, and the internal layer consists of VP2...

..late region of human papillomavirus type 6 (HPV-6) isolated in China and assembling capabilities of the encoded capsid proteins, HPV-6 L1 and L2 sequences were cloned and used for expression in Bac-to-Bac baculovirus expression ..

..These results support a model of APV capsid assembly in which complexes of the structural proteins VP1, VP3 (or VP2), and VP4, formed within the cytoplasm, ..

..Gag is then proteolyzed to produce the smaller capsid protein CA, which forms the central conical capsid that surrounds the RNA genome in the mature, infectious virus...

Infectious bursal disease virus (IBDV) is a nonenveloped virus with an icosahedral capsid composed of two proteins, VP2 and VP3, that derive from the processing of the polyprotein NH(2)-pVP2-VP4-VP3-COOH...

..Although tryptic cleavage of the VP2 N-terminus was not affected for the mutants, VP1 was degraded during proteolysis of mutant, but not wild-type, virions...

..mutants map to U(L)6, the gene encoding the protein subunit of the portal complex through which DNA enters the capsid. We tested the idea that WAY-150138 acts by preventing the incorporation of DNA-packaging proteins into capsids as ..

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..The 23K form is able to interact with the ORF2 capsid protein and be incorporated into virus-like particles...

..Alternatively, insertion of the myristate into the lipid bilayer might be a prerequisite event for the function of other distinct MA-encoded membrane binding domains...

The herpes simplex virus type 1 capsid is a protective shell that acts as a container for the genetic material of the virus...

..The results demonstrate a powerful new system for molecular genetic dissections of sigma1 with respect to its structure, assembly into particles, and roles in entry...

..These results indicate that the transmembrane domains of glycoproteins E2 and E1 of alphaviruses interact in a sequence-dependent manner and that this interaction is required for efficient budding and assembly of infectious virions...

..that the nuclear entry of simian virus 40 involves a dynamic interplay between two distinct interiorly situated capsid NLSs, the Vp1 NLS and the Vp3 NLS, and the selective exposure and importin recognition of the Vp3 NLS...

..The lipophilic belt contacts the non-matching symmetry vertex of the capsid and forms a bearing for the connector rotation...

..Escherichia coli leads to the formation of bona fide capsids, showing that the two histidines are not critical in capsid assembly...

Point mutations were introduced into the major capsid protein (P3) of cloned infectious cDNA of the polerovirus beet western yellows virus (BWYV) by manipulation of cloned infectious cDNA...

..morphological ramifications of a dominant epitope mutation and associated flanking amino acid substitutions on the capsid protein (CA) of SIV/simian-human immunodeficiency virus (SHIV)...

Overlapping DNA fragments containing the DNA packaging and capsid synthesis gene region of bacteriophage P2 were cloned and sequenced. In this report we present the complete nucleotide sequence of this 6550 bp region...

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..This system provides useful information for detailed studies of viral morphogenesis and the structural basis for the antigenicity of BKV...

..In the double-stranded DNA bacteriophages this cleavage is associated with DNA packaging, capsid expansion, and scaffold removal...

..Fifteen putative open reading frames and their respective amino acid sequences are described. One open reading frame is similar to the VP8 protein of rotavirus...

..We have analyzed 75 high-resolution virus capsid structures by calculating the average fraction of the solvent-accessible surface area of the coat protein subunits ..

..No serological relationships were found between the particles of the two viruses, although more than 50% of the amino acid sequences of the putative CPs are identical...

..These data suggest that incorporation of portal vertices into heads occurs during growth of the coat lattice at decision points that dictate head assembly fidelity...

..and virions to determine which component of the virus particle UL17 is associated with and at what stage in capsid assembly UL17 is required...

..The amino acid sequences of several peptide fragments of the major outer capsid protein reported as 45K were contained in the predicted polypeptide...

..None of these UL10 gene products is modified by N- or O-linked glycosylation. Isolation of a green fluorescent protein-expressing UL10 deletion mutant of ILTV revealed that this gene is not required for virus replication in cell culture...

..We have investigated the influence of the UL14 protein on the intracellular localization of capsid proteins and DNA cleavage and packaging proteins in coexpressing cells...

..of replicated concatemeric viral DNA into unit length genomes and their packaging into a preformed icosahedral capsid known as the procapsid...

..cleavage of the viral DNA (terminase activity); (ii) the UL6 protein may be an integral component of the capsid shell; and (iii) the UL25 protein may associate with capsids after scaffold loss and DNA packaging, sealing the ..

..1 portal protein encoded by UL6, the putative terminase components encoded by UL15, UL 28, and UL33, the minor capsid proteins encoded by UL17, and the major scaffold protein ICP35 were conducted...

..revealed that in cells transfected with a vlf-1 knockout bacmid, aberrant tubular structures containing the capsid protein vp39 were observed, suggesting that this virus construct was defective in producing mature capsids...

..Finding that DNA packaging is unimpeded in proheads containing the fusion argues that portal rotation is not crucial to DNA translocation. The paper is a landmark for describing direct testing of the mechanism of DNA translocation...

We examined how a particular type of intermolecular disulfide (ds) bond is formed in the capsid of a cytoplasmically replicating nonenveloped animal virus despite the normally reducing environment inside cells...

..While these viruses do not grow in tissue culture cells or animal models, expression of the capsid protein in insect cells results in the self-assembly of recombinant NV virus-like particles (rNV VLPs) that are ..

..The messenger release hypothesis predicts that RNA 3 must complement such an inoculum in order to produce RNA 4 that will trigger the process. This is shown indeed to be the case...

The L1 and L2 capsid proteins of animal and human papillomaviruses (HPVs) can self-assemble into virus-like particles (VLPs) that closely resemble native virions...

..to derive an Ad vector with enhanced targeting potential by a radical replacement of the fiber protein in the Ad capsid with a chimeric molecule containing a heterologous trimerization motif and a receptor-binding ligand...

..The reovirus penetration agent is the outer-capsid protein, Mu1...

..cerevisiae cultures from newly synthesized capsid proteins and also package newly synthesized DNA, including full-length and truncated viral DNA and S...

..to one that can support initiation of dsRNA synthesis requires the presence and partial assembly of the core capsid protein...

The herpes simplex virus type 1 UL6 protein forms a 12-subunit ring structure at a unique capsid vertex which functions as a conduit for encapsidation of the viral genome...

..The relationship between mutations of cleavage sites or C-terminal p6* residues and protease mutations suggests that these alterations may serve a compensatory role, increasing viral fitness...

..CA protein undergoes dramatic structural changes and establishes unique intermolecular interfaces in the mature capsid shell that are different from those that existed in the immature precursor...