potexvirus

Summary

Summary: A genus of plant viruses in the family FLEXIVIRIDAE, that cause mosaic and ringspot symptoms. Transmission occurs mechanically. Potato virus X is the type species.

Top Publications

  1. Kiselyova O, Yaminsky I, Karpova O, Rodionova N, Kozlovsky S, Arkhipenko M, et al. AFM study of potato virus X disassembly induced by movement protein. J Mol Biol. 2003;332:321-5 pubmed
    ..Our data supported the idea that binding of TGBp1 to one end of the PVX CP-helix induced linear destabilization of the whole helical particle, which may lead to its disassembly under conditions of AFM. ..
  2. Lin J, Hsu Y, Tsai C. Characterization of the infectivity of Bamboo mosaic virus with its correlation to the in vitro replicase activities in Nicotiana benthamiana. Virus Res. 2005;112:77-84 pubmed
    ..Results of exogenous RNA template activities using the 3'-ends of plus- and minus-strand RNA indicated that the 5th dpi RdRp could initiate minus-strand RNA synthesis more efficiency than the 11th dpi RdRp. ..
  3. Mumford R, Metcalfe E. The partial sequencing of the genomic RNA of a UK isolate of Pepino mosaic virus and the comparison of the coat protein sequence with other isolates from Europe and Peru. Arch Virol. 2001;146:2455-60 pubmed
    ..This analysis shows that all the tomato isolates share over 99% identity, but only between 96-97% identity with the Peruvian pepino isolate. ..
  4. Cooper B, Eckert D, Andon N, Yates J, Haynes P. Investigative proteomics: identification of an unknown plant virus from infected plants using mass spectrometry. J Am Soc Mass Spectrom. 2003;14:736-41 pubmed
    ..We believe this demonstrates that HPLC-MS/MS can be used to successfully characterize unknown viruses in infected plants. ..
  5. Candresse T, Marais A, Faure C, Dubrana M, Gombert J, Bendahmane A. Multiple coat protein mutations abolish recognition of Pepino mosaic potexvirus (PepMV) by the potato rx resistance gene in transgenic tomatoes. Mol Plant Microbe Interact. 2010;23:376-83 pubmed publisher
  6. Farnham G, Baulcombe D. Artificial evolution extends the spectrum of viruses that are targeted by a disease-resistance gene from potato. Proc Natl Acad Sci U S A. 2006;103:18828-33 pubmed
    ..The breadth of the disease-resistance phenotype from a natural R gene may be influenced by the tradeoff between the costs and benefits of broad-spectrum disease resistance. ..
  7. Cheng C, Hsiao Y, Wu H, Chuang C, Chen J, Tsai C, et al. Suppression of bamboo mosaic virus accumulation by a putative methyltransferase in Nicotiana benthamiana. J Virol. 2009;83:5796-805 pubmed publisher
    Bamboo mosaic virus (BaMV) is a 6.4-kb positive-sense RNA virus belonging to the genus Potexvirus of the family Flexiviridae...
  8. van Wezel R, Hong Y. Virus survival of RNA silencing without deploying protein-mediated suppression in Nicotiana benthamiana. FEBS Lett. 2004;562:65-70 pubmed
    ..Our results provide evidence that a plant virus may have developed a getaway strategy to survive RNA silencing. ..
  9. Gutierrez Aguirre I, Mehle N, Delić D, Gruden K, Mumford R, Ravnikar M. Real-time quantitative PCR based sensitive detection and genotype discrimination of Pepino mosaic virus. J Virol Methods. 2009;162:46-55 pubmed publisher

More Information

Publications120 found, 100 shown here

  1. Qiu W, Scholthof K. Satellite panicum mosaic virus capsid protein elicits symptoms on a nonhost plant and interferes with a suppressor of virus-induced gene silencing. Mol Plant Microbe Interact. 2004;17:263-71 pubmed
    ..Therefore, the roles of the SPMV CP now include encapsidation of the SPMV RNA, activity as a pathogenicity factor in both host and nonhost plants, and the enigmatic feature of interfering with suppression of gene silencing...
  2. Lu R, Malcuit I, Moffett P, Ruiz M, Peart J, Wu A, et al. High throughput virus-induced gene silencing implicates heat shock protein 90 in plant disease resistance. EMBO J. 2003;22:5690-9 pubmed
    ..This approach illustrates the potential benefits and limitations of RNA silencing in forward screens of gene function in plants. ..
  3. Hasiów Jaroszewska B, Borodynko N, Pospieszny H. Infectious RNA transcripts derived from cloned cDNA of a pepino mosaic virus isolate. Arch Virol. 2009;154:853-6 pubmed publisher
    ..The presence and authenticity of the progeny virus were verified by ELISA, RT-PCR and nucleotide sequencing. ..
  4. Lee C, Ho Y, Hu R, Yen Y, Wang Z, Lee Y, et al. The interaction between bamboo mosaic virus replication protein and coat protein is critical for virus movement in plant hosts. J Virol. 2011;85:12022-31 pubmed publisher
    Bamboo mosaic virus (BaMV) is a positive-sense RNA virus belonging to the genus Potexvirus. Open reading frame 1 (ORF1) encodes the viral replication protein that consists of a capping enzyme domain, a helicase-like domain (HLD), and an ..
  5. Rairdan G, Moffett P. Distinct domains in the ARC region of the potato resistance protein Rx mediate LRR binding and inhibition of activation. Plant Cell. 2006;18:2082-93 pubmed
    ..We propose instead that this activity can lead to multiple rounds of elicitor recognition, providing a means of signal amplification. ..
  6. Verchot Lubicz J, Ye C, Bamunusinghe D. Molecular biology of potexviruses: recent advances. J Gen Virol. 2007;88:1643-55 pubmed
    Recent advances in potexvirus research have produced new models describing virus replication, cell-to-cell movement, encapsidation, R gene-mediated resistance and gene silencing...
  7. Manske U, Schiemann J. Development and assessment of a potato virus X-based expression system with improved biosafety. Environ Biosafety Res. 2005;4:45-57 pubmed
    ..With respect to the stability of the gus insert and the expression level of the GUS protein, there were no differences between the novel system developed and the conventional PVX-based expression system. ..
  8. Ju H, Brown J, Ye C, Verchot Lubicz J. Mutations in the central domain of potato virus X TGBp2 eliminate granular vesicles and virus cell-to-cell trafficking. J Virol. 2007;81:1899-911 pubmed
    ..These results identify a novel element in the PVX TGBp2 protein which determines vesicle morphology. In addition, the data indicate that vesicles of the granular type induced by TGBp2 are necessary for PVX plasmodesmata transport. ..
  9. Lim H, Vaira A, Domier L, Lee S, Kim H, Hammond J. Efficiency of VIGS and gene expression in a novel bipartite potexvirus vector delivery system as a function of strength of TGB1 silencing suppression. Virology. 2010;402:149-63 pubmed publisher
    ..Substitution of TGB1 L(88)P, and equivalent changes in other potexvirus TGB1 proteins, affected RNA silencing suppression efficacy and suitability of the vectors from protein ..
  10. Lee Y, Hsu Y, Lin N. Generation of subgenomic RNA directed by a satellite RNA associated with bamboo mosaic potexvirus: analyses of potexvirus subgenomic RNA promoter. J Virol. 2000;74:10341-8 pubmed
    Satellite RNA of bamboo mosaic potexvirus (satBaMV), a single-stranded positive-sense RNA encoding a nonstructural protein of 20 kDa (P20), depends on bamboo mosaic potexvirus (BaMV) for replication and encapsidation...
  11. Dizadji A, Koohi Habibi M, Izadpanah K, Dietrich C, Mossahebi G, Winter S. Characterisation of lettuce virus X, a new potexvirus infecting lettuce in Iran. Arch Virol. 2008;153:1867-75 pubmed publisher
    ..properties and the complete genome sequence, this virus is a member of a new species in the genus Potexvirus, for which the name lettuce virus X (LeVX) is proposed...
  12. Hasiów Jaroszewska B, Borodynko N, Jackowiak P, Figlerowicz M, Pospieszny H. Single mutation converts mild pathotype of the Pepino mosaic virus into necrotic one. Virus Res. 2011;159:57-61 pubmed publisher
    Pepino mosaic virus (PepMV) is a member of the Flexiviridae family, genus Potexvirus. PepMV isolates from the same genotype differ in the symptoms they induce in Datura inoxia and Solanum lycopersicum...
  13. Lin M, Chang B, Liao J, Lin N, Hsu Y. Arg-16 and Arg-21 in the N-terminal region of the triple-gene-block protein 1 of Bamboo mosaic virus are essential for virus movement. J Gen Virol. 2004;85:251-9 pubmed
  14. Lin N, Hsu Y. A satellite RNA associated with bamboo mosaic potexvirus. Virology. 1994;202:707-14 pubmed publisher
    A small RNA molecule with properties of a satellite RNA was found in an isolate of bamboo mosaic potexvirus (BaMV-V) from Bambusa vulgaris McClure...
  15. Vaistij F, Jones L. Compromised virus-induced gene silencing in RDR6-deficient plants. Plant Physiol. 2009;149:1399-407 pubmed publisher
    ..For the potexvirus Potato virus X (PVX) and the potyvirus Plum pox virus (PPV), the efficiency of both VIGS and RdDM were ..
  16. Huang Y, Hu C, Lin C, Liu Y, Tsai C, Lin N, et al. Structural and functional analyses of the 3' untranslated region of Bamboo mosaic virus satellite RNA. Virology. 2009;386:139-53 pubmed publisher
    ..Taken together, these data indicate that satBaMV might have adopted a 3'-UTR structure similar to that of BaMV but may have evolved distinct features for its efficient replication. ..
  17. Lin N, Lin B, Lo N, Hu C, Chow T, Hsu Y. Nucleotide sequence of the genomic RNA of bamboo mosaic potexvirus. J Gen Virol. 1994;75 ( Pt 9):2513-8 pubmed publisher
    ..26 to 49%, 30 to 53%, 15 to 35% and 20 to 30%, respectively, to the corresponding ORFs of other members of the potexvirus group...
  18. Maroon Lango C, Guaragna M, Jordan R, Hammond J, Bandla M, Marquardt S. Two unique US isolates of Pepino mosaic virus from a limited source of pooled tomato tissue are distinct from a third (European-like) US isolate. Arch Virol. 2005;150:1187-201 pubmed
    ..the US have been cloned and sequenced by RT-PCR using total RNA from infected tissue as template, and degenerate potexvirus- and PepMV species- and isolate-specific primers...
  19. Leshchiner A, Minina E, Rakitina D, Vishnichenko V, Solovyev A, Morozov S, et al. Oligomerization of the potato virus X 25-kD movement protein. Biochemistry (Mosc). 2008;73:50-5 pubmed
    A 25-kD movement protein (25K protein) encoded by the first gene of the potexvirus Potato virus X triple gene block of transport genes is essential for the viral movement in infected plants...
  20. Chen H, Hsu Y, Lin N. Downregulation of Bamboo mosaic virus replication requires the 5' apical hairpin stem loop structure and sequence of satellite RNA. Virology. 2007;365:271-84 pubmed
  21. Senshu H, Ozeki J, Komatsu K, Hashimoto M, Hatada K, Aoyama M, et al. Variability in the level of RNA silencing suppression caused by triple gene block protein 1 (TGBp1) from various potexviruses during infection. J Gen Virol. 2009;90:1014-24 pubmed publisher
    ..In this study, we analysed the RNA silencing suppression ability of multiple virus species of the genus Potexvirus. Nicotiana benthamiana plants exhibiting RNA silencing of a green fluorescent protein (GFP) transgene showed ..
  22. Huang C, Huang Y, Meng M, Hsu Y, Tsai C. Sequences at the 3' untranslated region of bamboo mosaic potexvirus RNA interact with the viral RNA-dependent RNA polymerase. J Virol. 2001;75:2818-24 pubmed
    The 3' untranslated region (UTR) of bamboo mosaic potexvirus (BaMV) genomic RNA was found to fold into a series of stem-loop structures including a pseudoknot structure...
  23. Hong Y, Stanley J, van Wezel R. Novel system for the simultaneous analysis of geminivirus DNA replication and plant interactions in Nicotiana benthamiana. J Virol. 2003;77:13315-22 pubmed
    ..This defect correlates with possible loss of Rep nuclear localization and inability to trigger the host defense mechanism resembling a hypersensitive response. ..
  24. Chiu W, Hsu Y, Tsai C. Specificity analysis of the conserved hexanucleotides for the replication of bamboo mosaic potexvirus RNA. Virus Res. 2002;83:159-67 pubmed
    ..the potexviral conserved hexanucleotide sequence (ACc/uUAA) found in the 3' untranslated region of bamboo mosaic potexvirus (BaMV) genomic RNA, each nucleotide in the hexamer motif was substituted...
  25. Lukashina E, Badun G, Fedorova N, Ksenofontov A, Nemykh M, Serebryakova M, et al. Tritium planigraphy study of structural alterations in the coat protein of Potato virus X induced by binding of its triple gene block 1 protein to virions. FEBS J. 2009;276:7006-15 pubmed publisher
    ..A detailed description of the 'sandwich' version of the intravirus PVX coat protein model is presented...
  26. Cheng S, Huang Y, Wu Z, Hu C, Hsu Y, Tsai C. Identification of differentially expressed genes induced by Bamboo mosaic virus infection in Nicotiana benthamiana by cDNA-amplified fragment length polymorphism. BMC Plant Biol. 2010;10:286 pubmed publisher
    ..In this report, 9 of the 15 analyzed genes exhibited either a positive or a negative influence on the accumulation of BaMV in N. benthamiana plants. ..
  27. Kobayashi K, Sarrobert C, Ares X, Rivero M, Maldonado S, Robaglia C, et al. Over-expression of potato virus X TGBp1 movement protein in transgenic tobacco plants causes developmental and metabolic alterations. Plant Physiol Biochem. 2004;42:731-8 pubmed
    ..Since these changes were not observed when other PVX sequences were expressed in tobacco, we postulate that TGBp1 is an important symptom contributor in PVX infections. ..
  28. Karpova O, Zayakina O, Arkhipenko M, Sheval E, Kiselyova O, Poljakov V, et al. Potato virus X RNA-mediated assembly of single-tailed ternary 'coat protein-RNA-movement protein' complexes. J Gen Virol. 2006;87:2731-40 pubmed
    Different models have been proposed for the nature of the potexvirus transport form that moves from cell to cell over the infected plant: (i) genomic RNA moves as native virions; or (ii) in vitro-assembled non-virion ribonucleoprotein (..
  29. Hsu H, Tseng Y, Chou Y, Su S, Hsu Y, Chang B. Characterization of the RNA-binding properties of the triple-gene-block protein 2 of Bamboo mosaic virus. Virol J. 2009;6:50 pubmed publisher
    ..These results raise the possibility that TGBp2 facilitates intracellular delivery of viral RNA through non-specific protein-RNA interaction...
  30. Chiu M, Chen I, Baulcombe D, Tsai C. The silencing suppressor P25 of Potato virus X interacts with Argonaute1 and mediates its degradation through the proteasome pathway. Mol Plant Pathol. 2010;11:641-9 pubmed publisher
    ..Cell-to-cell movement of Potato Potexvirus X is dependent on suppression of RNA silencing. Plant J.44, 471-482)...
  31. Prasanth K, Huang Y, Liou M, Wang R, Hu C, Tsai C, et al. Glyceraldehyde 3-phosphate dehydrogenase negatively regulates the replication of Bamboo mosaic virus and its associated satellite RNA. J Virol. 2011;85:8829-40 pubmed publisher
    ..These observations support the contention that cytosolic GAPDH participates in the negative regulation of BaMV and satBaMV RNA replication. ..
  32. Lin M, Hu C, Lin N, Chang B, Hsu Y. Movement of potexviruses requires species-specific interactions among the cognate triple gene block proteins, as revealed by a trans-complementation assay based on the bamboo mosaic virus satellite RNA-mediated expression system. J Gen Virol. 2006;87:1357-67 pubmed
    ..The results indicate that species-specific interactions among movement proteins are obligatory for the cell-to-cell movement of BaMV and possibly other potexviruses. ..
  33. Wu X, Hou W, Wang M, Zhu X, Li F, Zhang J, et al. RNA silencing-mediated resistance is related to biotic / abiotic stresses and cellular RdRp expression in transgenic tobacco plants. BMB Rep. 2008;41:376-81 pubmed
    ..Collectively, we propose that biotic and abiotic stress factors affect RNA silencing-mediated resistance in transgenic tobacco plants and that their effects target different steps of RNA silencing. ..
  34. Li C, Zhang K, Zeng X, Jackson S, Zhou Y, Hong Y. A cis element within flowering locus T mRNA determines its mobility and facilitates trafficking of heterologous viral RNA. J Virol. 2009;83:3540-8 pubmed publisher
  35. Wu C, Lee S, Wang C. Viral protein targeting to the cortical endoplasmic reticulum is required for cell-cell spreading in plants. J Cell Biol. 2011;193:521-35 pubmed publisher
    ..Most of these proteins, including the triple-gene-block 3 protein (TGBp3) of Potexvirus, are ER membrane proteins...
  36. Howard A, Heppler M, Ju H, Krishnamurthy K, Payton M, Verchot Lubicz J. Potato virus X TGBp1 induces plasmodesmata gating and moves between cells in several host species whereas CP moves only in N. benthamiana leaves. Virology. 2004;328:185-97 pubmed
    ..The ability of GFP/CP and mutant GFP/TGBp1 fusion proteins to move in N. benthamiana and not in the other PVX host species suggests that N. benthamiana plants have a unique ability to promote protein intercellular movement...
  37. Pillai Nair N, Kim K, Hemenway C. Cis-acting regulatory elements in the potato virus X 3' non-translated region differentially affect minus-strand and plus-strand RNA accumulation. J Mol Biol. 2003;326:701-20 pubmed
    ..These data indicate that the PVX 3' NTR contains multiple, overlapping elements that influence accumulation of both minus and plus-strand RNA. ..
  38. Liu J, Lin N. Satellite RNA associated with bamboo mosaic potexvirus shares similarity with satellites associated with sobemoviruses. Arch Virol. 1995;140:1511-4 pubmed
    A putative nonstructural protein encoded by a satellite RNA associated with bamboo mosaic potexvirus shares 46% identity with the capsid protein of satellite virus of panicum mosaic sobemovirus...
  39. Liu J, Hsu Y, Huang T, Lin N. Molecular evolution and phylogeny of satellite RNA associated with bamboo mosaic potexvirus. J Mol Evol. 1997;44:207-13 pubmed
    Satellite RNA of bamboo mosaic potexvirus (satBaMV) is a linear RNA molecule which encodes a 20-kDa nonstructural protein. Sequences of seven different satBaMV isolates from bamboo hosts in three genera showed 0.7% to 7...
  40. Xie Z, Fan B, Chen C, Chen Z. An important role of an inducible RNA-dependent RNA polymerase in plant antiviral defense. Proc Natl Acad Sci U S A. 2001;98:6516-21 pubmed
    ..These results strongly suggest that inducible RdRP activity plays an important role in plant antiviral defense. ..
  41. Foster T, Lough T, Emerson S, Lee R, Bowman J, Forster R, et al. A surveillance system regulates selective entry of RNA into the shoot apex. Plant Cell. 2002;14:1497-508 pubmed
    ..These observations suggest the operation of an underlying regulatory mechanism. To examine this possibility, a potexvirus movement protein, known to modify cell-to-cell trafficking and PTGS, was expressed ectopically in transgenic ..
  42. Mechtcheriakova I, Eldarov M, Nicholson L, Shanks M, Skryabin K, Lomonossoff G. The use of viral vectors to produce hepatitis B virus core particles in plants. J Virol Methods. 2006;131:10-5 pubmed
    ..This approach provides a means whereby a variety of chimaeric particles can be assessed quickly and cheaply for various diagnostic and vaccine applications...
  43. Pacheco R, García Marcos A, Manzano A, de Lacoba M, Camañes G, García Agustín P, et al. Comparative analysis of transcriptomic and hormonal responses to compatible and incompatible plant-virus interactions that lead to cell death. Mol Plant Microbe Interact. 2012;25:709-23 pubmed publisher
    ..Furthermore, silencing of coronatine insensitive 1, a gene involved in jasmonate perception, in N. benthamiana accelerated cell death induced by PVX expressing HC-Pro. ..
  44. Fedorkin O, Solovyev A, Yelina N, Zamyatnin A, Zinovkin R, Makinen K, et al. Cell-to-cell movement of potato virus X involves distinct functions of the coat protein. J Gen Virol. 2001;82:449-58 pubmed
  45. Nemykh M, Efimov A, Novikov V, Orlov V, Arutyunyan A, Drachev V, et al. One more probable structural transition in potato virus X virions and a revised model of the virus coat protein structure. Virology. 2008;373:61-71 pubmed
    ..A revised model of the three-dimensional organization of coat protein subunits in the PVX virions is proposed. This two-domain model explains better the high plasticity of the PVX CP structure. ..
  46. Kwon S, Park M, Kim K, Plante C, Hemenway C, Kim K. cis-Acting sequences required for coat protein binding and in vitro assembly of Potato virus X. Virology. 2005;334:83-97 pubmed
    ..Taken together, our results suggest that RNA secondary structural elements within SL1 and/or sequences therein are crucial for formation of VLPs and are required for the specific recognition by the CP subunit. ..
  47. Tilsner J, Linnik O, Christensen N, Bell K, Roberts I, Lacomme C, et al. Live-cell imaging of viral RNA genomes using a Pumilio-based reporter. Plant J. 2009;57:758-70 pubmed publisher
    ..The utility of Pumilio as a fluorescence-based reporter for viral RNA is discussed. ..
  48. Chen I, Chou W, Lee P, Hsu Y, Tsai C. The AAUAAA motif of bamboo mosaic virus RNA is involved in minus-strand RNA synthesis and plus-strand RNA polyadenylation. J Virol. 2005;79:14555-61 pubmed
    ..Taken together, the results suggest that the AAUAAA motif in the 3' UTR of BaMV genomic RNA is involved not only in the formation of the poly(A) tail of the plus-strand RNA, but also in minus-strand RNA synthesis. ..
  49. Gomez P, Sempere R, Elena S, Aranda M. Mixed infections of Pepino mosaic virus strains modulate the evolutionary dynamics of this emergent virus. J Virol. 2009;83:12378-87 pubmed publisher
    ..Thus, mixed infections appear to be contributing to shaping the genetic structure and dynamics of PepMV populations. ..
  50. Lin N, Lee Y, Lin B, Lee C, Hsu Y. The open reading frame of bamboo mosaic potexvirus satellite RNA is not essential for its replication and can be replaced with a bacterial gene. Proc Natl Acad Sci U S A. 1996;93:3138-42 pubmed
    A satellite RNA of 836 nt depends on the bamboo mosaic potexvirus (BaMV) for its replication and encapsulation. The BaMV satellite RNA (satBaMV) contains a single open reading frame encoding a 20-kDa nonstructural protein...
  51. Li Y, Chen Y, Hsu Y, Meng M. Characterization of the AdoMet-dependent guanylyltransferase activity that is associated with the N terminus of bamboo mosaic virus replicase. J Virol. 2001;75:782-8 pubmed
    Bamboo mosaic virus (BaMV), a member of the potexvirus group, infects primarily members of the Bambusoideae...
  52. Bamunusinghe D, Hemenway C, Nelson R, Sanderfoot A, Ye C, Silva M, et al. Analysis of potato virus X replicase and TGBp3 subcellular locations. Virology. 2009;393:272-85 pubmed publisher
    ..Cerulenin, a drug that inhibits de novo membrane synthesis, also inhibited PVX replication. These combined data indicate that PVX replication relies on ER-derived membrane recruitment and membrane proliferation...
  53. Sharma P, Ikegami M. Tomato leaf curl Java virus V2 protein is a determinant of virulence, hypersensitive response and suppression of posttranscriptional gene silencing. Virology. 2010;396:85-93 pubmed publisher
    ..This finding demonstrated that ToLCJV-A[ID] V2 is a pathogenicity determinant that elicits an HR-like response...
  54. Tsai C, Cheng C, Peng C, Lin B, Lin N, Hsu Y. Sufficient length of a poly(A) tail for the formation of a potential pseudoknot is required for efficient replication of bamboo mosaic potexvirus RNA. J Virol. 1999;73:2703-9 pubmed
    RNAs transcribed from a full-length infectious cDNA clone of the bamboo mosaic potexvirus (strain O) genome, pBaMV-O, were infectious to Nicotiana benthamiana plants...
  55. Lough T, Netzler N, Emerson S, Sutherland P, Carr F, Beck D, et al. Cell-to-cell movement of potexviruses: evidence for a ribonucleoprotein complex involving the coat protein and first triple gene block protein. Mol Plant Microbe Interact. 2000;13:962-74 pubmed
    ..movement and encapsidation functions of the CP were shown to be separable, and the rate-limiting factor of potexvirus movement was shown not to be virion accumulation, but rather, the presence of TGBp1-3 and the CP in the infected ..
  56. Tremblay M, Majeau N, Gagné M, Lecours K, Morin H, Duvignaud J, et al. Effect of mutations K97A and E128A on RNA binding and self assembly of papaya mosaic potexvirus coat protein. FEBS J. 2006;273:14-25 pubmed
    Papaya mosaic potexvirus (PapMV) coat protein (CP) was expressed (CPdeltaN5) in Escherichia coli and showed to self assemble into nucleocapsid like particles (NLPs)...
  57. Lough T, Lee R, Emerson S, Forster R, Lucas W. Functional analysis of the 5' untranslated region of potexvirus RNA reveals a role in viral replication and cell-to-cell movement. Virology. 2006;351:455-65 pubmed
    ..cis-acting motifs required for recognition and translocation of viral RNA were identified using an artificial potexvirus defective RNA encoding a green fluorescent protein (GFP) reporter transcriptionally fused to the terminal viral ..
  58. Zayakina O, Arkhipenko M, Kozlovsky S, Nikitin N, Smirnov A, Susi P, et al. Mutagenic analysis of potato virus X movement protein (TGBp1) and the coat protein (CP): in vitro TGBp1-CP binding and viral RNA translation activation. Mol Plant Pathol. 2008;9:37-44 pubmed publisher
    ..The evidence to discriminate between these two events, i.e. TGBp1 binding to the CP-helix and TGBp1-dependent RNA translation activation, is discussed...
  59. Lin K, Cheng C, Chang B, Wang W, Huang Y, Lee Y, et al. Global analyses of small interfering RNAs derived from Bamboo mosaic virus and its associated satellite RNAs in different plants. PLoS ONE. 2010;5:e11928 pubmed publisher
    ..Here, we report on the small RNA profiles associated with infection with Bamboo mosaic virus (BaMV) and its two satellite RNAs (satBaMVs) in Nicotiana benthamiana and Arabidopsis thaliana...
  60. Atabekov J, Rodionova N, Karpova O, Kozlovsky S, Poljakov V. The movement protein-triggered in situ conversion of potato virus X virion RNA from a nontranslatable into a translatable form. Virology. 2000;271:259-63 pubmed
    ..The process of complex formation is ATP-independent; i.e., the ATPase activity of the 25-kDa MP is not involved in the binding of the MP to PVX virion...
  61. Cheng J, Peng C, Hsu Y, Tsai C. The synthesis of minus-strand RNA of bamboo mosaic potexvirus initiates from multiple sites within the poly(A) tail. J Virol. 2002;76:6114-20 pubmed
    The 3' terminus of the bamboo mosaic potexvirus (BaMV) contains a poly(A) tail, the 5' portion of which participates in the formation of an RNA pseudoknot required for BaMV RNA replication...
  62. Moffett P, Farnham G, Peart J, Baulcombe D. Interaction between domains of a plant NBS-LRR protein in disease resistance-related cell death. EMBO J. 2002;21:4511-9 pubmed
    ..We propose that activation of Rx entails sequential disruption of at least two intramolecular interactions...
  63. Krishnamurthy K, Mitra R, Payton M, Verchot Lubicz J. Cell-to-cell movement of the PVX 12K, 8K, or coat proteins may depend on the host, leaf developmental stage, and the PVX 25K protein. Virology. 2002;300:269-81 pubmed
    ..Thus plasmodesmata gating is not an essential function of these proteins for virus cell-to-cell movement. These proteins likely provide additional activities for virus cell-to-cell movement...
  64. Bayne E, Rakitina D, Morozov S, Baulcombe D. Cell-to-cell movement of potato potexvirus X is dependent on suppression of RNA silencing. Plant J. 2005;44:471-82 pubmed
    ..Here, through random mutation analysis of the Potato Potexvirus X (PVX) silencing suppressor P25, we provide evidence that it does...
  65. Chen I, Meng M, Hsu Y, Tsai C. Functional analysis of the cloverleaf-like structure in the 3' untranslated region of bamboo mosaic potexvirus RNA revealed dual roles in viral RNA replication and long distance movement. Virology. 2003;315:415-24 pubmed
    The 3' untranslated region (UTR) of bamboo mosaic potexvirus (BaMV) RNA was identified to fold into a tertiary structure comprising a cloverleaf-like structure designated ABC domain followed by a major stem-loop D, which in turn is ..
  66. Roth B, Pruss G, Vance V. Plant viral suppressors of RNA silencing. Virus Res. 2004;102:97-108 pubmed
    ..Because they interfere with silencing at different points in the pathway, these viral suppressors are powerful tools to help unravel the mechanism of RNA silencing in plants...
  67. Schwach F, Vaistij F, Jones L, Baulcombe D. An RNA-dependent RNA polymerase prevents meristem invasion by potato virus X and is required for the activity but not the production of a systemic silencing signal. Plant Physiol. 2005;138:1842-52 pubmed
    ..According to this idea, the secondary siRNAs mediate RNA silencing as an immediate response that slows down the systemic spreading of the virus into the growing point and newly emerging leaves...
  68. Ju H, Samuels T, Wang Y, Blancaflor E, Payton M, Mitra R, et al. The potato virus X TGBp2 movement protein associates with endoplasmic reticulum-derived vesicles during virus infection. Plant Physiol. 2005;138:1877-95 pubmed
    ..We propose a model in which reorganization of the ER and increased protein degradation is linked to plasmodesmata gating...
  69. Chapman S, Kavanagh T, Baulcombe D. Potato virus X as a vector for gene expression in plants. Plant J. 1992;2:549-57 pubmed
    ..These data point to a general utility of PVX as a vector for unregulated gene expression in plants...
  70. Cheng J, Ding M, Hsu Y, Tsai C. The partial purified RNA-dependent RNA polymerases from bamboo mosaic potexvirus and potato virus X infected plants containing the template-dependent activities. Virus Res. 2001;80:41-52 pubmed
    RNA-dependent RNA polymerases (RdRp) isolated from bamboo mosaic potexvirus (BaMV) and potato virus X infected Nicotiana benthamiana plants and solubilized with the detergent NP-40, generated a full-length genomic and two subgenomic ..
  71. Tamai A, Meshi T. Cell-to-cell movement of Potato virus X: the role of p12 and p8 encoded by the second and third open reading frames of the triple gene block. Mol Plant Microbe Interact. 2001;14:1158-67 pubmed
    ..Considering the likelihood that p12 and p8 are membrane proteins, it is suggested that intercellular as well as intracellular movement of PVX involves a membrane-mediated process...
  72. Bucher E, Hemmes H, de Haan P, Goldbach R, Prins M. The influenza A virus NS1 protein binds small interfering RNAs and suppresses RNA silencing in plants. J Gen Virol. 2004;85:983-91 pubmed
    ..In addition, NS1 was shown to be capable of binding siRNAs. The data presented here fit with a potential role for NS1 in counteracting innate antiviral responses in vertebrates by sequestering siRNAs...
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    ..This level of sensitivity indicated that the one-step immuno-capture duo-primer TaqMan real-time RT-PCR developed in the present study could be used for routine seed health assays...
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    ..Taken together, these data suggest that the sRNA deep sequencing technology will likely become an efficient and powerful generic tool for virus identification in plants and animals...
  75. Che J, Zheng H, Chen J, Adams M. Characterisation of a potyvirus and a potexvirus from Chinese scallion. Arch Virol. 2002;147:683-93 pubmed
    ..The complete sequences of a potyvirus and a potexvirus were determined and these were shown to represent different viruses from any in the databases...
  76. Aguilar J, Hern ndez Gallardo M, Cenis J, Lacasa A, Aranda M. Complete sequence of the Pepino mosaic virus RNA genome. Arch Virol. 2002;147:2009-15 pubmed publisher
    ..Thus, the genome organization of PepMV is that of a typical member of the genus Potexvirus. The nucleotide sequence obtained shares an overall 99% identity with the genomic RNA of a PepMV isolate from UK ..
  77. Cotillon A, Girard M, Ducouret S. Complete nucleotide sequence of the genomic RNA of a French isolate of Pepino mosaic virus (PepMV). Brief report. Arch Virol. 2002;147:2231-8 pubmed
    ..degrees of similarity of PepMV proteins with those of other potexviruses confirmed that PepMV belongs to the Potexvirus genus...
  78. Krishnamurthy K, Heppler M, Mitra R, Blancaflor E, Payton M, Nelson R, et al. The Potato virus X TGBp3 protein associates with the ER network for virus cell-to-cell movement. Virology. 2003;309:135-51 pubmed
    ..Plasmids expressing GFP:TGBp2 and GFP:TGBp3 were bombarded to several other PVX hosts and neither protein moved between adjacent cells. In most hosts, TGBp2 or TGBp3 cannot move cell-to-cell...
  79. Rodionova N, Karpova O, Kozlovsky S, Zayakina O, Arkhipenko M, Atabekov J. Linear remodeling of helical virus by movement protein binding. J Mol Biol. 2003;333:565-72 pubmed
    ..e. that intersubunit conformational changes may be transferred along the CP helix...
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    ..However, integrity of the C-terminal hydrophilic sequence is required for entrance to this non-canonical route...
  81. Samuels T, Ju H, Ye C, Motes C, Blancaflor E, Verchot Lubicz J. Subcellular targeting and interactions among the Potato virus X TGB proteins. Virology. 2007;367:375-89 pubmed
    ..These experiments indicate that the PVX TGB proteins have complex subcellular accumulation patterns and likely cooperate across subcellular compartments to promote virus infection...
  82. Tsai M, Hsu Y, Lin N. Bamboo mosaic potexvirus satellite RNA (satBaMV RNA)-encoded P20 protein preferentially binds to satBaMV RNA. J Virol. 1999;73:3032-9 pubmed
    A satellite RNA of 836 nucleotides [excluding the poly(A) tail] depends on the bamboo mosaic potexvirus (BaMV) for its replication and encapsidation...
  83. Roggero P, Ciuffo M, Benvenuto E, Franconi R. The expression of a single-chain Fv antibody fragment in different plant hosts and tissues by using Potato virus X as a vector. Protein Expr Purif. 2001;22:70-4 pubmed
    ..quinoa. No correlation was found between PVX and scFv concentration in the inoculated and systemically invaded leaves of N. benthamiana and N. clevelandii...
  84. Marusic C, Rizza P, Lattanzi L, Mancini C, Spada M, Belardelli F, et al. Chimeric plant virus particles as immunogens for inducing murine and human immune responses against human immunodeficiency virus type 1. J Virol. 2001;75:8434-9 pubmed
    ..Thus, PVX-based CVPs carrying neutralizing epitopes can offer novel perspectives for the development of effective vaccines against HIV and, more generally, for the design of new vaccination strategies in humans...
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    ..Moreover, covariation analyses of natural satBaMV variants provide substantial evidence that the secondary structure in the 5'-UTR of satBaMV is necessary for efficient replication...
  86. Komarova T, Skulachev M, Zvereva A, Schwartz A, Dorokhov Y, Atabekov J. New viral vector for efficient production of target proteins in plants. Biochemistry (Mosc). 2006;71:846-50 pubmed
    ..The novel vector can be used for production of different proteins including pharmaceuticals in plants...
  87. Hsu H, Chou Y, Tseng Y, Lin Y, Lin T, Lin N, et al. Topological properties of the triple gene block protein 2 of Bamboo mosaic virus. Virology. 2008;379:1-9 pubmed publisher
    ..Moreover, TGBp2 is able to self-assemble as revealed by the significant increase in multimeric TGBp2 when the TGBp2-containing proteoliposomes were treated with chemical crosslinker or oxidation agent...
  88. Raffaele S, Bayer E, Lafarge D, Cluzet S, German Retana S, Boubekeur T, et al. Remorin, a solanaceae protein resident in membrane rafts and plasmodesmata, impairs potato virus X movement. Plant Cell. 2009;21:1541-55 pubmed publisher
    ..Based on the localization of REM and its impact on virus macromolecular trafficking, we discuss the potential for lipid rafts to act as functional components in plasmodesmata and the plasma membrane...
  89. Hasiów Jaroszewska B, Jackowiak P, Borodynko N, Figlerowicz M, Pospieszny H. Quasispecies nature of Pepino mosaic virus and its evolutionary dynamics. Virus Genes. 2010;41:260-7 pubmed publisher
    ..The observed level of genetic variability allows PepMV for a quick and flexible adaptation to different hosts. Our results suggest that the level of PepMV variability possibly influences the course of infection...
  90. van Wezel R, Liu H, Tien P, Stanley J, Hong Y. Gene C2 of the monopartite geminivirus tomato yellow leaf curl virus-China encodes a pathogenicity determinant that is localized in the nucleus. Mol Plant Microbe Interact. 2001;14:1125-8 pubmed
    ..Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized...
  91. Li Y, Shih T, Hsu Y, Han Y, Huang Y, Meng M. The helicase-like domain of plant potexvirus replicase participates in formation of RNA 5' cap structure by exhibiting RNA 5'-triphosphatase activity. J Virol. 2001;75:12114-20 pubmed
  92. Kalinina N, Rakitina D, Solovyev A, Schiemann J, Morozov S. RNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block. Virology. 2002;296:321-9 pubmed
    ..A recombinant His-tagged form of TGBp1 of two hordeiviruses and potato virus X, a potexvirus, produced in Escherichia coli had unwinding activity on a partially duplexed RNA, but not DNA substrate...