tospovirus

Summary

Summary: A genus of plant viruses in the family BUNYAVIRIDAE. Tomato spotted wilt virus is the type species. Transmission occurs by at least nine species of thrips.

Top Publications

  1. Sin S, McNulty B, Kennedy G, Moyer J. Viral genetic determinants for thrips transmission of Tomato spotted wilt virus. Proc Natl Acad Sci U S A. 2005;102:5168-73 pubmed
    ..These results also indicate that the glycoproteins may not be required for TSWV infection of plant hosts but are required for transmissibility by thrips...
  2. Prins M, Lohuis D, Schots A, Goldbach R. Phage display-selected single-chain antibodies confer high levels of resistance against Tomato spotted wilt virus. J Gen Virol. 2005;86:2107-13 pubmed
    ..Protoplast experiments provided evidence that the two resistance-conferring antibodies may have a different mode of action and could be combined for higher durability of resistance in the field. ..
  3. Hassani Mehraban A, Brenkman A, van den Broek N, Goldbach R, Kormelink R. RNAi-mediated transgenic Tospovirus resistance broken by intraspecies silencing suppressor protein complementation. Mol Plant Microbe Interact. 2009;22:1250-7 pubmed publisher
    ..transgene cassette, containing partial nucleoprotein (N) gene sequences from four different tomato-infecting Tospovirus spp...
  4. de Oliveira A, Bertran A, Inoue Nagata A, Nagata T, Kitajima E, Oliveira Resende R. An RNA-dependent RNA polymerase gene of a distinct Brazilian tospovirus. Virus Genes. 2011;43:385-9 pubmed publisher
    ..In this study, an L segment sequence of 9,040 bp from a new tospovirus (family Bunyaviridae) naturally infecting bean (Phaseolus vulgaris L.) plants was characterized...
  5. Geerts Dimitriadou C, Lu Y, Geertsema C, Goldbach R, Kormelink R. Analysis of the Tomato spotted wilt virus ambisense S RNA-encoded hairpin structure in translation. PLoS ONE. 2012;7:e31013 pubmed publisher
    ..The presence of this hairpin structure sequence in the 3' untranslated region (UTR) of TSWV mRNAs implies a possible role in translation...
  6. Moritz G, Kumm S, Mound L. Tospovirus transmission depends on thrips ontogeny. Virus Res. 2004;100:143-9 pubmed
    ..Mechanical transmission through excrement and oviposition by adults is a possible alternative mode of virus transmission that requires investigation...
  7. Medeiros R, Resende R, de Avila A. The plant virus Tomato Spotted Wilt Tospovirus activates the immune system of its main insect vector, Frankliniella occidentalis. J Virol. 2004;78:4976-82 pubmed
    ..Tomato spotted wilt virus (TSWV), the type species in the Tospovirus genus, infects its most important insect vector, Frankliniella occidentalis, the western flower thrips (WFT)...
  8. Lewandowski D, Adkins S. The tubule-forming NSm protein from Tomato spotted wilt virus complements cell-to-cell and long-distance movement of Tobacco mosaic virus hybrids. Virology. 2005;342:26-37 pubmed
    ..Our combination of single-cell and intact plant experiments to examine multiple functions of a heterologous viral protein provides a generalized strategy with wider application to other viruses also lacking a reverse genetic system...
  9. Kahn N, Walgenbach J, Kennedy G. Summer weeds as hosts for Frankliniella occidentalis and Frankliniella fusca (Thysanoptera: Thripidae) and as reservoirs for tomato spotted wilt Tospovirus in North Carolina. J Econ Entomol. 2005;98:1810-5 pubmed
    In North Carolina, Tomato spotted wilt tospovirus (family Bunyaviridae, genus Tospovirus, TSWV) is vectored primarily by the tobacco thrips, Frankliniella fusca (Hinds), and the western flower thrips, Frankliniella occidentalis (Pergande) ..

More Information

Publications104 found, 100 shown here

  1. Chen T, Li J, Fan Y, Yeh Y, Yeh S, Kormelink R. Molecular characterization of the full-length L and M RNAs of Tomato yellow ring virus, a member of the genus Tospovirus. Virus Genes. 2013;46:487-95 pubmed publisher
    ..ring virus (TYRV), first isolated from tomato in Iran, was classified as a non-approved species of the genus Tospovirus based on the characterization of its genomic S RNA...
  2. Heinze C, Letschert B, Hristova D, Yankulova M, Kauadjouor O, Willingmann P, et al. Variability of the N-protein and the intergenic region of the S RNA of tomato spotted wilt tospovirus (TSWV). New Microbiol. 2001;24:175-87 pubmed
  3. Kritzman A, Gera A, Raccah B, van Lent J, Peters D. The route of tomato spotted wilt virus inside the thrips body in relation to transmission efficiency. Arch Virol. 2002;147:2143-56 pubmed
    ..These results support the contention that the virus must reach the salivary glands before thrips pupation in order to be transmitted by old second instar larvae and adults...
  4. van Knippenberg I, Lamine M, Goldbach R, Kormelink R. Tomato spotted wilt virus transcriptase in vitro displays a preference for cap donors with multiple base complementarity to the viral template. Virology. 2005;335:122-30 pubmed
    ..For Tomato spotted wilt virus (TSWV), type species of the plant-infecting Tospovirus genus within the Bunyaviridae, cap donors were previously shown to require a single base complementarity to the ..
  5. van Knippenberg I, Goldbach R, Kormelink R. Tomato spotted wilt virus S-segment mRNAs have overlapping 3'-ends containing a predicted stem-loop structure and conserved sequence motif. Virus Res. 2005;110:125-31 pubmed
    ..This suggests that these viral transcripts contain a predicted stem-loop structure at their 3'-end. The potential involvement of the 3'-end structure in transcription termination is discussed...
  6. Kunkalikar S, Poojari S, Arun B, Rajagopalan P, Chen T, Yeh S, et al. Importance and genetic diversity of vegetable-infecting tospoviruses in India. Phytopathology. 2011;101:367-76 pubmed publisher
    ..A proposal for establishing WBNV as a distinct tospovirus species is made based on the molecular characterization of small- (S) and medium- (M) RNA segments.
  7. Dietzgen R, Martin K, Anderson G, Goodin M. In planta localization and interactions of impatiens necrotic spot tospovirus proteins. J Gen Virol. 2012;93:2490-5 pubmed publisher
    Impatiens necrotic spot tospovirus (INSV) is a significant pathogen of ornamentals...
  8. Satyanarayana T, Mitchell S, Reddy D, Brown S, Kresovich S, Jarret R, et al. Peanut bud necrosis tospovirus S RNA: complete nucleotide sequence, genome organization and homology to other tospoviruses. Arch Virol. 1996;141:85-98 pubmed
    ..5 kDa protein, identified as the nonstructural (NSs) protein based on similarity to published tospovirus sequences. The second ORF (831 nucleotides in virus complementary strand) encodes a Mr 30.6 kDa protein...
  9. Takeda A, Sugiyama K, Nagano H, Mori M, Kaido M, Mise K, et al. Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus. FEBS Lett. 2002;532:75-9 pubmed
    ..TSWV NSs protein suppressed sense transgene-induced PTGS but did not suppress inverted repeat transgene-induced PTGS. TSWV NSs protein is the first RNA silencing suppressor identified in negative-strand RNA viruses...
  10. Rinne P, Van Den Boogaard R, Mensink M, Kopperud C, Kormelink R, Goldbach R, et al. Tobacco plants respond to the constitutive expression of the tospovirus movement protein NS(M) with a heat-reversible sealing of plasmodesmata that impairs development. Plant J. 2005;43:688-707 pubmed
    ..This type of defence may also play a role in the formation of symptoms during viral infection...
  11. Garcia S, Billecocq A, Crance J, Prins M, Garin D, Bouloy M. Viral suppressors of RNA interference impair RNA silencing induced by a Semliki Forest virus replicon in tick cells. J Gen Virol. 2006;87:1985-9 pubmed
    ..in ticks, three known viral inhibitors that act in different ways, the NS1 protein of Influenza virus, NSs of Tospovirus Tomato spotted wilt virus and HC-Pro of Zucchini yellow mosaic virus were expressed and investigated to ..
  12. Beaudoin A, Kahn N, Kennedy G. Bell and banana pepper exhibit mature-plant resistance to tomato spotted wilt Tospovirus transmitted by Frankliniella fusca (Thysanoptera: Thripidae). J Econ Entomol. 2009;102:30-5 pubmed
    Tomato spotted wilt virus (family Bunyaviridae, genus Tospovirus, TSWV) causes annual economic losses in pepper, Capsicum annuum L...
  13. Schnettler E, Hemmes H, Huismann R, Goldbach R, Prins M, Kormelink R. Diverging affinity of tospovirus RNA silencing suppressor proteins, NSs, for various RNA duplex molecules. J Virol. 2010;84:11542-54 pubmed publisher
    The tospovirus NSs protein was previously shown to suppress the antiviral RNA silencing mechanism in plants...
  14. Oliveira V, Bartasson L, de Castro M, Corrêa J, Ribeiro B, Resende R. A silencing suppressor protein (NSs) of a tospovirus enhances baculovirus replication in permissive and semipermissive insect cell lines. Virus Res. 2011;155:259-67 pubmed publisher
    ..Our data showed that NSs protein of TSWV facilitates baculovirus replication in different lepidopteran cell lines, and these results indicate that NSs could play a similar role during TSWV-infection in its thrips vector...
  15. Seepiban C, Gajanandana O, Attathom T, Attathom S. Tomato necrotic ringspot virus, a new tospovirus isolated in Thailand. Arch Virol. 2011;156:263-74 pubmed publisher
    A new tospovirus isolated from naturally infected tomato plants grown in Nakhon Pathom province (Thailand) was characterized. Infected plants showed symptoms consisting of necrotic spots, necrotic ringspots and stem necrosis...
  16. Satyanarayana T, Mitchell S, Reddy D, Kresovich S, Jarret R, Naidu R, et al. The complete nucleotide sequence and genome organization of the M RNA segment of peanut bud necrosis tospovirus and comparison with other tospoviruses. J Gen Virol. 1996;77 ( Pt 9):2347-52 pubmed publisher
    ..Defective RNA molecules derived from the genomic M RNA were detected during continuous passage of the virus by sap inoculations...
  17. Cortez I, Saaijer J, Wongjkaew K, Pereira A, Goldbach R, Peters D, et al. Identification and characterization of a novel tospovirus species using a new RT-PCR approach. Arch Virol. 2001;146:265-78 pubmed
    A novel tospovirus serologically distinct from all established tospovirus species was found in Thailand in Physalis minima L. The S RNA of this virus was cloned by a new RT-PCR approach revealing a nucleotide sequence of 3257 nucleotides...
  18. Bag S, Druffel K, Pappu H. Structure and genome organization of the large RNA of iris yellow spot virus (genus Tospovirus, family Bunyaviridae). Arch Virol. 2010;155:275-9 pubmed publisher
    ..spot virus (IYSV) was determined, and with this report, the complete genomic sequence of IYSV of the genus Tospovirus, family Bunyaviridae has been elucidated...
  19. Tentchev D, Verdin E, Marchal C, Jacquet M, Aguilar J, Moury B. Evolution and structure of Tomato spotted wilt virus populations: evidence of extensive reassortment and insights into emergence processes. J Gen Virol. 2011;92:961-73 pubmed publisher
    Tomato spotted wilt virus (TSWV; genus Tospovirus, family Bunyaviridae) genetic diversity was evaluated by sequencing parts of the three RNA genome segments of 224 isolates, mostly from pepper and tomato crops in southern Europe...
  20. Zhou J, Kantartzi S, Wen R, Newman M, Hajimorad M, Rupe J, et al. Molecular characterization of a new Tospovirus infecting soybean. Virus Genes. 2011;43:289-95 pubmed publisher
    ..Protein pairwise comparisons coupled with phylogenetic analyses indicate that the virus is a new member of the genus Tospovirus.
  21. Abe H, Tomitaka Y, Shimoda T, Seo S, Sakurai T, Kugimiya S, et al. Antagonistic plant defense system regulated by phytohormones assists interactions among vector insect, thrips and a tospovirus. Plant Cell Physiol. 2012;53:204-12 pubmed publisher
    ..Our results suggest the following mechanism: TSWV infection suppresses the anti-herbivore response in plants and attracts its vector, thrips, to virus-infected plants by exploiting the antagonistic SA-JA plant defense systems...
  22. Gowda S, Satyanarayana T, Naidu R, Mushegian A, Dawson W, Reddy D. Characterization of the large (L) RNA of peanut bud necrosis tospovirus. Arch Virol. 1998;143:2381-90 pubmed
    ..purified from peanut plants systemically infected with peanut bud necrosis virus (PBNV), a member of the genus Tospovirus, contained both viral(v) and viral complementary(vc) sense L RNAs...
  23. van Knippenberg I, Goldbach R, Kormelink R. Purified tomato spotted wilt virus particles support both genome replication and transcription in vitro. Virology. 2002;303:278-86 pubmed
    ..Moreover, an artificially produced N mRNA containing an AMV-derived leader was shown to be used as cap donor, indicating that resnatching of viral mRNAs takes place in vitro...
  24. Bucher E, Sijen T, de Haan P, Goldbach R, Prins M. Negative-strand tospoviruses and tenuiviruses carry a gene for a suppressor of gene silencing at analogous genomic positions. J Virol. 2003;77:1329-36 pubmed
    ..These results indicate that plant-infecting negative-strand RNA viruses carry a gene for a suppressor of RNA silencing...
  25. Tsompana M, Abad J, Purugganan M, Moyer J. The molecular population genetics of the Tomato spotted wilt virus (TSWV) genome. Mol Ecol. 2005;14:53-66 pubmed
    ..to characterize the evolution of a highly pathogenic plant RNA virus [Tomato spotted wilt virus (TSWV), Tospovirus, Bunyaviridae]...
  26. Snippe M, Willem Borst J, Goldbach R, Kormelink R. Tomato spotted wilt virus Gc and N proteins interact in vivo. Virology. 2007;357:115-23 pubmed
    ..The genuine character of N-Gc interaction was confirmed by its presence in purified virus and RNP preparations. The results are discussed in view of TSWV particle assembly taking place at the Golgi complex...
  27. Pappu H, Jones R, Jain R. Global status of tospovirus epidemics in diverse cropping systems: successes achieved and challenges ahead. Virus Res. 2009;141:219-36 pubmed publisher
    The diseases caused by thrips-transmitted tospoviruses (genus Tospovirus, family Bunyaviridae) are a major constraint to production of important vegetable, legume and ornamental crops in different parts of the world...
  28. Hassani Mehraban A, Botermans M, Verhoeven J, Meekes E, Saaijer J, Peters D, et al. A distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia. Arch Virol. 2010;155:423-8 pubmed publisher
    A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests...
  29. Lokesh B, Rashmi P, Amruta B, Srisathiyanarayanan D, Murthy M, Savithri H. NSs encoded by groundnut bud necrosis virus is a bifunctional enzyme. PLoS ONE. 2010;5:e9757 pubmed publisher
    Groundnut bud necrosis virus (GBNV), a member of genus Tospovirus in the family Bunyaviridae, infects a large number of leguminosae and solanaceae plants in India...
  30. Duijsings D, Kormelink R, Goldbach R. In vivo analysis of the TSWV cap-snatching mechanism: single base complementarity and primer length requirements. EMBO J. 2001;20:2545-52 pubmed
    ..Cleavage occurred preferentially at an A residue, suggesting a requirement for a single base complementarity with the TSWV RNA template, which could be confirmed by analyses of host mRNAs used in vivo as cap donors...
  31. Whitfield A, Ullman D, German T. Tospovirus-thrips interactions. Annu Rev Phytopathol. 2005;43:459-89 pubmed
    ..In this review, we focus primarily on recent progress toward understanding the biological processes and molecular interactions involved in the acquisition and transmission of Tospoviruses by their thrips vectors...
  32. Hassani Mehraban A, Saaijer J, Peters D, Goldbach R, Kormelink R. Molecular and biological comparison of two Tomato yellow ring virus (TYRV) isolates: challenging the Tospovirus species concept. Arch Virol. 2007;152:85-96 pubmed
    Two strains of Tomato yellow ring virus (TYRV, genus Tospovirus), one from tomato (referred to as TYRV-t) and the other from soybean and potato (denoted TYRV-s), collected from different geographical regions in Iran, were compared...
  33. Bucher E, Lohuis D, van Poppel P, Geerts Dimitriadou C, Goldbach R, Prins M. Multiple virus resistance at a high frequency using a single transgene construct. J Gen Virol. 2006;87:3697-701 pubmed
    ..Thus RNA silencing can be further improved for high frequency multiple virus resistance by combining small RNA fragments from a series of target viruses...
  34. Dong J, Cheng X, Yin Y, Fang Q, Ding M, Li T, et al. Characterization of tomato zonate spot virus, a new tospovirus in China. Arch Virol. 2008;153:855-64 pubmed publisher
    An isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties...
  35. Hagen C, Frizzi A, Kao J, Jia L, Huang M, Zhang Y, et al. Using small RNA sequences to diagnose, sequence, and investigate the infectivity characteristics of vegetable-infecting viruses. Arch Virol. 2011;156:1209-16 pubmed publisher
    ..We repeated this technique in identification of a squash-infecting geminivirus and also used it to identify an unspecified tospovirus.
  36. Polischuk V, Budzanivska I, Shevchenko T, Oliynik S. Evidence for plant viruses in the region of Argentina Islands, Antarctica. FEMS Microbiol Ecol. 2007;59:409-17 pubmed
    ..However, the means of plant virus emergence in the region remain elusive and are discussed. ..
  37. Zheng Y, Chen C, Jan F. Complete nucleotide sequence of capsicum chlorosis virus isolated from Phalaenopsis orchid and the prediction of the unexplored genetic information of tospoviruses. Arch Virol. 2011;156:421-32 pubmed publisher
    Phalaenopsis orchids are popular ornamentals all over the world. A tospovirus, capsicum chlorosis virus (CaCV-Ph) had been identified as the cause of chlorotic ringspots on leaves of Phalaenopsis orchids in Taiwan...
  38. Czech A, Szklarczyk M, Gajewski Z, Zukowska E, Michalik B, Kobyłko T, et al. Selection of tomato plants resistant to a local Polish isolate of tomato spotted wilt virus (TSWV). J Appl Genet. 2003;44:473-80 pubmed
    ..Some observed discrepancies of these data are also discussed. Our results indicate that marker-assisted selection can be used for breeding of the TSWV-resistant tomato in Poland. ..
  39. Kovalenko O, Shepelevych V. [Pathogenesis and induced virus resistance in tobacco plants affected by tomato spotted wilt virus]. Mikrobiol Z. 2004;66:81-5 pubmed
    ..It has been shown that strongly virulent isolates interfere with weakly virulent ones and induce the development of nonspecific resistance in tobacco plants, this resistance is also efficient to the tobacco mosaic virus. ..
  40. Zhang Y, Zhang C, Li W. The nucleocapsid protein of an enveloped plant virus, Tomato spotted wilt virus, facilitates long-distance movement of Tobacco mosaic virus hybrids. Virus Res. 2012;163:246-53 pubmed publisher
    ..On the basis of these findings, the potential role of N during the TSWV infection was discussed. To our knowledge, this is the first report that the N protein of an enveloped plant virus functioned in long-distance movement...
  41. Zhu M, Jiang L, Bai B, Zhao W, Chen X, Li J, et al. The Intracellular Immune Receptor Sw-5b Confers Broad-Spectrum Resistance to Tospoviruses through Recognition of a Conserved 21-Amino Acid Viral Effector Epitope. Plant Cell. 2017;29:2214-2232 pubmed publisher
    ..in the Sw-5b LRR domain were positively selected during evolution and are all necessary to confer resistance to tospovirus. The results described here provide a new example of a plant NLR mediating broad-spectrum resistance through ..
  42. Frese M, Prins M, Ponten A, Goldbach R, Haller O, Zeltz P. Constitutive expression of interferon-induced human MxA protein in transgenic tobacco plants does not confer resistance to a variety of RNA viruses. Transgenic Res. 2000;9:429-38 pubmed
    ..spot virus, and groundnut ringspot virus revealed no increased resistance of MxA-transgenic tobacco plants to tospovirus infections...
  43. Kovalenko O, Shepelevich V. [Changes in ultrastructure and protein metabolism of tobacco plants infected by tomato spotted wilt virus]. Tsitol Genet. 2003;37:24-9 pubmed
    ..At the same time it induces formation of at least three pathogenesis-associated proteins (PR-proteins) and two antiviral factors of the types AVF (6) and IVR (7) active towards tobacco mosaic virus...
  44. Rao X, Wu Z, Li Y. Complete genome sequence of a Watermelon silver mottle virus isolate from China. Virus Genes. 2013;46:576-80 pubmed publisher
    The complete genome of a Watermelon silver mottle virus (WSMoV) (genus Tospovirus, family Bunyaviridae) isolate (WSMoV-GZ) from Guangdong province, China was sequenced...
  45. Inoue T, Sakurai T, Murai T, Maeda T. Specificity of accumulation and transmission of tomato spotted wilt virus (TSWV) in two genera, Frankliniella and Thrips (Thysanoptera: Thripidae). Bull Entomol Res. 2004;94:501-7 pubmed
  46. Pappu H, du Toit L, Schwartz H, Mohan S. Sequence diversity of the nucleoprotein gene of iris yellow spot virus (genus Tospovirus, family Bunyaviridae) isolates from the western region of the United States. Arch Virol. 2006;151:1015-23 pubmed
    Iris yellow spot virus (IYSV), a tentative virus species in the genus Tospovirus and family Bunyaviridae, is considered a rapidly emerging threat to onion production in the western United States (US)...
  47. Mandal B, Mandal S, Csinos A, Martinez N, Culbreath A, Pappu H. Biological and molecular analyses of the acibenzolar-S-methyl-induced systemic acquired resistance in flue-cured tobacco against Tomato spotted wilt virus. Phytopathology. 2008;98:196-204 pubmed publisher
    ..Use of gibberellic acid in combination with ASM reduced the stunting caused by ASM. Present findings together with previous field experiments demonstrate that ASM is a potential option for management of TSWV in flue-cured tobacco. ..
  48. Smith E, Fuchs M, Shields E, Nault B. Long-Distance Dispersal Potential for Onion Thrips (Thysanoptera: Thripidae) and Iris yellow spot virus (Bunyaviridae: Tospovirus) in an Onion Ecosystem. Environ Entomol. 2015;44:921-30 pubmed publisher
    ..Our results indicate that T. tabaci adults, including viruliferous individuals, engage in long-distance dispersal late in the season and likely contribute to the spread of IYSV. ..
  49. Kumar R, Mandal B, Geetanjali A, Jain R, Jaiwal P. Genome organisation and sequence comparison suggest intraspecies incongruence in M RNA of Watermelon bud necrosis virus. Arch Virol. 2010;155:1361-5 pubmed publisher
    Watermelon bud necrosis virus (WBNV), a member of the genus Tospovirus, family Bunyaviridae is an important viral pathogen in watermelon cultivation in India. The complete genome sequence properties of WBNV are not available...
  50. Liu L, Ye H, Chen T, Chen T. Development of a microarray for simultaneous detection and differentiation of different tospoviruses that are serologically related to Tomato spotted wilt virus. Virol J. 2017;14:1 pubmed publisher
    ..Tomato spotted wilt virus (TSWV) is the type species of the genus Tospovirus and one of the most destructive plant viruses...
  51. Corpillo D, Gardini G, Vaira A, Basso M, Aime S, Accotto G, et al. Proteomics as a tool to improve investigation of substantial equivalence in genetically modified organisms: the case of a virus-resistant tomato. Proteomics. 2004;4:193-200 pubmed
    ..Fifteen proteins were identified by peptide mass fingerprinting. ..
  52. Diaz Montano J, Fuchs M, Nault B, Shelton A. Evaluation of onion cultivars for resistance to onion thrips (Thysanoptera: Thripidae) and Iris yellow spot virus. J Econ Entomol. 2010;103:925-37 pubmed
    ..by > 50% and be even more problematic when it transmits Iris yellow spot virus (family Bunyaviridae, genus Tospovirus, IYSV). Because T...
  53. Zhang Z, Zhang P, Li W, Zhang J, Huang F, Yang J, et al. De novo transcriptome sequencing in Frankliniella occidentalis to identify genes involved in plant virus transmission and insecticide resistance. Genomics. 2013;101:296-305 pubmed publisher
    ..occidentalis. This data-set not only enriches genomic resource for WFT, but also benefits research into its molecular genetics and functional genomics. ..
  54. Hedil M, Kormelink R. Viral RNA Silencing Suppression: The Enigma of Bunyavirus NSs Proteins. Viruses. 2016;8: pubmed publisher
    The Bunyaviridae is a family of arboviruses including both plant- and vertebrate-infecting representatives. The Tospovirus genus accommodates plant-infecting bunyaviruses, which not only replicate in their plant host, but also in their ..
  55. Ogada P, Kiirika L, Lorenz C, Senkler J, Braun H, Poehling H. Differential proteomics analysis of Frankliniella occidentalis immune response after infection with Tomato spotted wilt virus (Tospovirus). Dev Comp Immunol. 2017;67:1-7 pubmed publisher
    ..Key proteins included: 70 kDa heat shock proteins, Ubiquitin and Dermcidin, among others, indicative of a responsive pattern of the vector's innate immune system to viral infection. ..
  56. Snippe M, Smeenk L, Goldbach R, Kormelink R. The cytoplasmic domain of tomato spotted wilt virus Gn glycoprotein is required for Golgi localisation and interaction with Gc. Virology. 2007;363:272-9 pubmed
    ..Truncated Gn constructs lacking the C-terminal 20 amino acids but still localising to the Golgi were no longer able to redirect Gc, suggesting the requirement of this domain for interaction with Gc. ..
  57. Ramesh S, Williams S, Kappagantu M, Mitter N, Pappu H. Transcriptome-wide identification of host genes targeted by tomato spotted wilt virus-derived small interfering RNAs. Virus Res. 2017;238:13-23 pubmed publisher
    ..of highly expressed viral small interfering RNAs (vsiRNAs) derived from the tomato spotted wilt virus (TSWV; Tospovirus: Bunyaviridae) genome was analyzed for their propensity to downregulate the tomato transcriptome...
  58. Ribeiro D, Foresti O, Denecke J, Wellink J, Goldbach R, Kormelink R. Tomato spotted wilt virus glycoproteins induce the formation of endoplasmic reticulum- and Golgi-derived pleomorphic membrane structures in plant cells. J Gen Virol. 2008;89:1811-8 pubmed publisher
    ..The behaviour of both glycoproteins within the plant cell and the phenomenon of membrane deformation are discussed in light of the natural process of viral infection. ..
  59. García Marcos A, Pacheco R, Manzano A, Aguilar E, Tenllado F. Oxylipin biosynthesis genes positively regulate programmed cell death during compatible infections with the synergistic pair potato virus X-potato virus Y and Tomato spotted wilt virus. J Virol. 2013;87:5769-83 pubmed publisher
    ..We conclude that oxylipin metabolism is a critical component that positively regulates the process of PCD during compatible plant-virus interactions but does not play a role in restraining virus accumulation in planta. ..
  60. Nagata T, Inoue Nagata A, Van Lent J, Goldbach R, Peters D. Factors determining vector competence and specificity for transmission of Tomato spotted wilt virus. J Gen Virol. 2002;83:663-71 pubmed
  61. Hurt C, Brandenburg R, Jordan D, Kennedy G, Bailey J. Management of spotted wilt vectored by Frankliniella fusca (Thysanoptera: Thripidae) in Virginia market-type peanut. J Econ Entomol. 2005;98:1435-40 pubmed
    ..the impact of cultural practices and in-furrow insecticides on the incidence of Tomato spotted wilt virus (genus Tospovirus, family Bunyaviridae, TSWV), which is transmitted to peanut, Arachis hypogaea L...
  62. Margaria P, Ciuffo M, Pacifico D, Turina M. Evidence that the nonstructural protein of Tomato spotted wilt virus is the avirulence determinant in the interaction with resistant pepper carrying the TSW gene. Mol Plant Microbe Interact. 2007;20:547-58 pubmed
    ..Analysis of another RB strain phenotype allowed us to conclude that local necrotic response is not sufficient for resistance in Capsicum spp. carrying the Tsw gene. ..
  63. Kunkalikar S, Sudarsana P, Rajagopalan P, Zehr U, Ravi K. Biological and molecular characterization of Capsicum chlorosis virus infecting chilli and tomato in India. Arch Virol. 2010;155:1047-57 pubmed publisher
    Two isolates of Capsicum chlorosis virus (CaCV, genus Tospovirus) from tomato (CaCV-To-Ind) and chilli (CaCV-Ch-Pan), collected from Haryana and Uttar Pradesh states of northern India respectively, were compared...
  64. Curtin S, Zsögön A, Watson J, Waterhouse P. Isolation and analysis of small RNAs from virus-infected plants. Methods Mol Biol. 2012;894:173-89 pubmed publisher
    ..EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA)...
  65. Taki A, Yamagishi N, Yoshikawa N. Development of apple latent spherical virus-based vaccines against three tospoviruses. Virus Res. 2013;176:251-8 pubmed publisher
    ..DNA fragments of 201 nt of three tospovirus S-RNAs (silencing suppressor (NSS) and nucleocapsid protein (N) coding regions for each tospovirus) were ..
  66. de Breuil S, Canizares J, Blanca J, Bejerman N, Trucco V, Giolitti F, et al. Analysis of the coding-complete genomic sequence of groundnut ringspot virus suggests a common ancestor with tomato chlorotic spot virus. Arch Virol. 2016;161:2311-6 pubmed publisher
    ..The high level of sequence similarity between GRSV and TCSV (over 90 % identity) observed in the genes and proteins encoded in the M RNA support previous results indicating that these viruses probably have a common ancestor. ..
  67. Shrestha A, Champagne D, Culbreath A, Rotenberg D, Whitfield A, Srinivasan R. Transcriptome changes associated with Tomato spotted wilt virus infection in various life stages of its thrips vector, Frankliniella fusca (Hinds). J Gen Virol. 2017;98:2156-2170 pubmed publisher
    ..The pathosystem components included a Tospovirus, Tomato spotted wilt virus (TSWV), its insect vector, Frankliniella fusca (Hinds), and a plant host, Arachis ..
  68. Komoda K, Narita M, Yamashita K, Tanaka I, Yao M. Asymmetric Trimeric Ring Structure of the Nucleocapsid Protein of Tospovirus. J Virol. 2017;91: pubmed publisher
    i>Tomato spotted wilt virus (TSWV), belonging to the genus Tospovirus of the family Bunyaviridae, causes significant economic damage to several vegetables and ornamental plants worldwide...
  69. Jain R, Pandey A, Krishnareddy M, Mandal B. Immunodiagnosis of groundnut and watermelon bud necrosis viruses using polyclonal antiserum to recombinant nucleocapsid protein of Groundnut bud necrosis virus. J Virol Methods. 2005;130:162-4 pubmed
    ..The antiserum to the NP of GBNV at 1:4000 dilution detected successfully natural infection of GBNV and Watermelon bud necrosis virus in a wide range of cucurbitaceous, leguminous and solanaceous hosts from different locations. ..
  70. Ribeiro D, Borst J, Goldbach R, Kormelink R. Tomato spotted wilt virus nucleocapsid protein interacts with both viral glycoproteins Gn and Gc in planta. Virology. 2009;383:121-30 pubmed publisher
    ..The observations are discussed in the context of the virus particle formation during the infection process. ..
  71. Bag S, Druffel K, Salewsky T, Pappu H. Nucleotide sequence and genome organization of the medium RNA of Iris yellow spot virus from the United States. Arch Virol. 2009;154:715-8 pubmed publisher
    Iris yellow spot tospovirus (IYSV) of the family Bunyaviridae causes a serious disease in onion in the USA and other parts of the world...
  72. Li W, Lewandowski D, Hilf M, Adkins S. Identification of domains of the Tomato spotted wilt virus NSm protein involved in tubule formation, movement and symptomatology. Virology. 2009;390:110-21 pubmed publisher
    ..These findings are likely applicable to other tospovirus species, especially those within the 'New World' group as NSm sequences are highly conserved.
  73. Miozzi L, Catoni M, Fiorilli V, Mullineaux P, Accotto G, Lanfranco L. Arbuscular mycorrhizal symbiosis limits foliar transcriptional responses to viral infection and favors long-term virus accumulation. Mol Plant Microbe Interact. 2011;24:1562-72 pubmed publisher
    ..However, in a longer period, increase in virus titer and delay in the appearance of recovery were observed in mycorrhizal plants, thus indicating that the plant's reaction to TSWV infection is attenuated by mycorrhization. ..
  74. Ma H, Song C, Borth W, Sether D, Melzer M, Hu J. Modified expression of alternative oxidase in transgenic tomato and petunia affects the level of tomato spotted wilt virus resistance. BMC Biotechnol. 2011;11:96 pubmed publisher
  75. Shang W, Meng C, Zheng K, Ding M, Zhang Z, Hong J, et al. [Expression and subcellular location of NSm protein of Tomato spotted wilt virus in plant and insect cells]. Wei Sheng Wu Xue Bao. 2012;52:962-8 pubmed
    ..These findings suggest that NSm protein target the plasmodesmata specifically in plant cells, and they also could form tubular structures on the surface when expressed in entomic Tn cells. ..
  76. Zhang Z, Wang D, Yu C, Wang Z, Dong J, Shi K, et al. Identification of three new isolates of Tomato spotted wilt virus from different hosts in China: molecular diversity, phylogenetic and recombination analyses. Virol J. 2016;13:8 pubmed publisher
    ..During their evolution, both reassortment and recombination played roles. These results suggest that recombination could be an important mechanism in the evolution of multipartite RNA viruses, even negative-sense RNA viruses. ..
  77. Zhang M, Schillberg S, Zimmermann S, Liao Y, Breuer G, Fischer R. GST fusion proteins cause false positives during selection of viral movement protein specific single chain antibodies. J Virol Methods. 2001;91:139-47 pubmed
  78. Okuda M, Hanada K. RT-PCR for detecting five distinct Tospovirus species using degenerate primers and dsRNA template. J Virol Methods. 2001;96:149-56 pubmed
    RT-PCR procedures for detection of multiple species of tospovirus from plant tissues were investigated. Downstream primers were designated from the 3' untranslated sequences of the S RNA...
  79. Levin J, Thompson W, Csinos A, Stephenson M, Weissinger A. Matrix attachment regions increase the efficiency and stability of RNA-mediated resistance to tomato spotted wilt virus in transgenic tobacco. Transgenic Res. 2005;14:193-206 pubmed
    ..These results are consistent with the concept that MARs may prevent transcriptional silencing...
  80. Kormelink R, Garcia M, Goodin M, Sasaya T, Haenni A. Negative-strand RNA viruses: the plant-infecting counterparts. Virus Res. 2011;162:184-202 pubmed publisher
    ..Genetic differences with the animal-infecting counterparts and their evolutionary descendants will be described in light of the above processes...
  81. Stankovic I, Bulajić A, Vučurović A, Ristic D, Milojevic K, Berenji J, et al. Status of tobacco viruses in Serbia and molecular characterization of tomato spotted wilt virus isolates. Acta Virol. 2011;55:337-47 pubmed
    ..The high incidence of viruses in Serbian tobacco crops highlights the importance of enhancing farmers knowledge towards better implementation of control strategies for preventing serious losses...
  82. Kellmann J, Liebisch P, Schmitz K, Piechulla B. Visual representation by atomic force microscopy (AFM) of tomato spotted wilt virus ribonucleoproteins. Biol Chem. 2001;382:1559-62 pubmed
    ..Hence, the AFM approach provides a starting point for further detailed studies on TSWV ribonucleoprotein complexes...
  83. Naidu R, Ingle C, Deom C, Sherwood J. The two envelope membrane glycoproteins of Tomato spotted wilt virus show differences in lectin-binding properties and sensitivities to glycosidases. Virology. 2004;319:107-17 pubmed
    Tomato spotted wilt virus (TSWV, Genus: Tospovirus, Family: Bunyaviridae) is a major constraint to the production of several different crops of agronomic and horticultural importance worldwide...
  84. Riley D, Pappu H. Tactics for management of thrips (Thysanoptera: Thripidae) and tomato spotted wilt virus in tomato. J Econ Entomol. 2004;97:1648-58 pubmed
    ..Control tactics that manage thrips early in the growing season significantly increased tomato yield in years when the incidence of TSWV was high (>17%)...
  85. Chiemsombat P, Gajanandana O, Warin N, Hongprayoon R, Bhunchoth A, Pongsapich P. Biological and molecular characterization of tospoviruses in Thailand. Arch Virol. 2008;153:571-7 pubmed publisher
    ..their 93% identity to CaCV isolates in the other two clades, CaCV-TD8, CaCV-AIT and CaCV-KS16-Thailand tomato tospovirus were designated as CaCV-tomato necrosis strain...
  86. Olatinwo R, Paz J, Brown S, Kemerait R, Culbreath A, Beasley J, et al. A predictive model for spotted wilt epidemics in peanut based on local weather conditions and the tomato spotted wilt virus risk index. Phytopathology. 2008;98:1066-74 pubmed publisher
    Tomato spotted wilt virus (TSWV), a member of the genus Tospovirus (family Bunyaviridae), is an important plant virus that causes severe damage to peanut (Arachis hypogaea) in the southeastern United States...
  87. Zilian E, Maiss E. An optimized mRFP-based bimolecular fluorescence complementation system for the detection of protein-protein interactions in planta. J Virol Methods. 2011;174:158-65 pubmed publisher
    ..This optimized BiFC system provides a convenient alternative to other BiFC, as well as yeast two-hybrid assays, for detecting protein-protein interactions...
  88. Hu Z, Feng Z, Zhang Z, Liu Y, Tao X. Complete genome sequence of a tomato spotted wilt virus isolate from China and comparison to other TSWV isolates of different geographic origin. Arch Virol. 2011;156:1905-8 pubmed publisher
    ..The phylogenetic relationship of the Chinese TSWV-YN isolate to other TSWV isolates of different geographic origin, based on the nucleotide sequences of the glycoprotein (GP) and nucleocapsid (N) genes, was also analyzed in this study...
  89. Srinivasan R, Sundaraj S, Pappu H, Diffie S, Riley D, Gitaitis R. Transmission of Iris yellow spot virus by Frankliniella fusca and Thrips tabaci (Thysanoptera: Thripidae). J Econ Entomol. 2012;105:40-7 pubmed
    Thrips-transmitted Iris yellow spot virus (IYSV) (Family Bunyaviridae, Genus Tospovirus) affects onion production in the United States and worldwide. The presence of IYSV in Georgia was confirmed in 2003...
  90. Mertelík J, Mokrá V. Tomato spotted wilt virus in ornamental plants, vegetables and weeds in the Czech Republic. Acta Virol. 1998;42:347-51 pubmed
    ..Among weeds, the TSWV infection occurred very often in Stellaria media and Galinsoga parviflora. These two plant species were prevalent in glasshouses and were also good hosts of F. occidentalis...
  91. Heinze C, Willingmann P, Schwach F, Adam G. An unusual large intergenic region in the S-RNA of a Bulgarian tomato spotted wilt virus isolate. Arch Virol. 2003;148:199-205 pubmed
    The complete S-RNA sequences of four Bulgarian and one German isolate of tomato spotted wilt tospovirus were determined...
  92. Hallwass M, Leastro M, Lima M, Inoue Nagata A, Resende R. Sequence determination and analysis of the NSs genes of two tospoviruses. Arch Virol. 2012;157:591-6 pubmed publisher
    ..6% identity with that of ZLCV and 75.9% identity with that of TSWV, while the NSs sequence of ZLCV and TSWV shared 67.9% identity. Phylogenetic analysis based on NSs sequences confirmed that these viruses cluster in the American clade...