Summary: A genus of plant viruses that infects ANGIOSPERMS. Transmission occurs mechanically and through soil, with one species transmitted via a fungal vector. The type species is Tomato bushy stunt virus.

Top Publications

  1. Panavas T, Pogany J, Nagy P. Internal initiation by the cucumber necrosis virus RNA-dependent RNA polymerase is facilitated by promoter-like sequences. Virology. 2002;296:275-87 pubmed
    ..An unusual feature of the tombusvirus RdRp that is partially purified from cucumber necrosis virus (CNV)-infected plants is the ability to initiate ..
  2. Pogany J, Stork J, Li Z, Nagy P. In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70. Proc Natl Acad Sci U S A. 2008;105:19956-61 pubmed publisher
    ..The assembled TBSV replicase performed a complete replication cycle, synthesizing RNA complementary to the provided RNA replicon and using the complementary RNA as template to synthesize new TBSV replicon RNA...
  3. Kovalev N, Pogany J, Nagy P. Template role of double-stranded RNA in tombusvirus replication. J Virol. 2014;88:5638-51 pubmed publisher
    ..This strategy likely allows TBSV to protect the replicating viral RNA from degradation as well as to evade the early detection of viral dsRNAs by the host surveillance system. ..
  4. Panavas T, Panaviene Z, Pogany J, Nagy P. Enhancement of RNA synthesis by promoter duplication in tombusviruses. Virology. 2003;310:118-29 pubmed studied in vitro by using a partially purified RNA-dependent RNA polymerase (RdRp) preparation obtained from tombusvirus-infected plants, Virology 276, 279- 288)...
  5. Cheng C, Serviene E, Nagy P. Suppression of viral RNA recombination by a host exoribonuclease. J Virol. 2006;80:2631-40 pubmed
    ..Altogether, this paper establishes that host ribonucleases and host-mediated viral RNA turnover play major roles in RNA virus recombination and evolution. ..
  6. Panavas T, Stork J, Nagy P. Use of double-stranded RNA templates by the tombusvirus replicase in vitro: Implications for the mechanism of plus-strand initiation. Virology. 2006;352:110-20 pubmed
  7. Pogany J, Nagy P. Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast. J Virol. 2008;82:5967-80 pubmed publisher
    To study the replication of Tomato bushy stunt virus (TBSV), a small tombusvirus of plants, we have developed a cell-free system based on a Saccharomyces cerevisiae extract...
  8. Barajas D, Li Z, Nagy P. The Nedd4-type Rsp5p ubiquitin ligase inhibits tombusvirus replication by regulating degradation of the p92 replication protein and decreasing the activity of the tombusvirus replicase. J Virol. 2009;83:11751-64 pubmed publisher
    ..Overall, our data suggest that direct binding between Rsp5p and p92(pol) reduces the stability of p92(pol), with consequent inhibition of TBSV replicase activity. ..
  9. Nicholson B, Zaslaver O, Mayberry L, Browning K, White K. Tombusvirus Y-shaped translational enhancer forms a complex with eIF4F and can be functionally replaced by heterologous translational enhancers. J Virol. 2013;87:1872-83 pubmed publisher
    ..Here, we have investigated the properties of the Y-shaped class of 3'CITE present in the tombusvirus Carnation Italian ringspot virus (CIRV)...

More Information

Publications123 found, 100 shown here

  1. Panavas T, Nagy P. Yeast as a model host to study replication and recombination of defective interfering RNA of Tomato bushy stunt virus. Virology. 2003;314:315-25 pubmed
    ..In summary, development of yeast as a host for replication of TBSV DI RNA will facilitate studies on the roles of viral and host proteins in replication/recombination. ..
  2. Celix A, Rodriguez Cerezo E, Garcia Arenal F. New satellite RNAs, but no DI RNAs, are found in natural populations of tomato bushy stunt tombusvirus. Virology. 1997;239:277-84 pubmed
    A collection of 57 field isolates of the tombusvirus tomato bushy stunt virus was obtained from eggplant and tomato during 1994-1997 and was examined for the presence of defective interfering (DI) RNA species by Northern blot ..
  3. Panaviene Z, Nagy P. Mutations in the RNA-binding domains of tombusvirus replicase proteins affect RNA recombination in vivo. Virology. 2003;317:359-72 pubmed
    ..In this article, we show evidence that the replicase proteins of Cucumber necrosis virus, a tombusvirus, are directly involved in RNA recombination in vivo...
  4. Omarov R, Ciomperlik J, Scholthof H. RNAi-associated ssRNA-specific ribonucleases in Tombusvirus P19 mutant-infected plants and evidence for a discrete siRNA-containing effector complex. Proc Natl Acad Sci U S A. 2007;104:1714-9 pubmed
  5. Scholthof H, Alvarado V, Vega Arreguín J, Ciomperlik J, Odokonyero D, Brosseau C, et al. Identification of an ARGONAUTE for antiviral RNA silencing in Nicotiana benthamiana. Plant Physiol. 2011;156:1548-55 pubmed publisher
    ..Thus, the role of NbAGO2 might be primarily for antiviral defense. ..
  6. Sharma M, Sasvari Z, Nagy P. Inhibition of phospholipid biosynthesis decreases the activity of the tombusvirus replicase and alters the subcellular localization of replication proteins. Virology. 2011;415:141-52 pubmed publisher
    ..These data are consistent with the important role of phospholipids in RNA virus replication...
  7. Barajas D, Nagy P. Ubiquitination of tombusvirus p33 replication protein plays a role in virus replication and binding to the host Vps23p ESCRT protein. Virology. 2010;397:358-68 pubmed publisher
    ..In this article, we identify two lysines in the tombusvirus p33 replication co-factor involved in ubiquitination and show that the same lysines are also important for the ..
  8. Wang S, Mortazavi L, White K. Higher-order RNA structural requirements and small-molecule induction of tombusvirus subgenomic mRNA transcription. J Virol. 2008;82:3864-71 pubmed publisher
    ..These findings provide important new insights into the premature termination mechanism and present a novel approach to regulate the transcriptional process. ..
  9. Havelda Z, Hornyik C, Crescenzi A, Burgyan J. In situ characterization of Cymbidium Ringspot Tombusvirus infection-induced posttranscriptional gene silencing in Nicotiana benthamiana. J Virol. 2003;77:6082-6 pubmed
    ..In contrast to the necrotic symptoms caused by wild-type tombusvirus, the infection of p19-defective mutant virus results in the development of a typical PTGS-associated recovery ..
  10. Nicholson B, Wu B, Chevtchenko I, White K. Tombusvirus recruitment of host translational machinery via the 3' UTR. RNA. 2010;16:1402-19 pubmed publisher
    ..Moreover, the virus-host interplay defined in this study provides insights into natural host resistance mechanisms that have been linked to 3'CITE activity. ..
  11. Cimino P, Nicholson B, Wu B, Xu W, White K. Multifaceted regulation of translational readthrough by RNA replication elements in a tombusvirus. PLoS Pathog. 2011;7:e1002423 pubmed publisher
    ..Based on comparative sequence analysis and experimental data, basic elements of this regulatory model extend to other members of Tombusviridae, as well as to viruses outside of this family. ..
  12. Li Z, Gonzalez P, Sasvari Z, Kinzy T, Nagy P. Methylation of translation elongation factor 1A by the METTL10-like See1 methyltransferase facilitates tombusvirus replication in yeast and plants. Virology. 2014;448:43-54 pubmed publisher
    ..eukaryotic translation elongation factor 1A (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex...
  13. Gursinsky T, Schulz B, Behrens S. Replication of Tomato bushy stunt virus RNA in a plant in vitro system. Virology. 2009;390:250-60 pubmed publisher
    ..Our studies with cell-free extracts of a plant host thus confirmed earlier findings and enabled novel insights into the TBSV RNA replication process. ..
  14. Wang S, White K. Riboswitching on RNA virus replication. Proc Natl Acad Sci U S A. 2007;104:10406-11 pubmed
    ..Through rational design, a tombusvirus RE, which has the structure of a perfect RNA stem loop in the plus-strand RNA genome, was replaced with a ..
  15. Havelda Z, Várallyay E, Valoczi A, Burgyan J. Plant virus infection-induced persistent host gene downregulation in systemically infected leaves. Plant J. 2008;55:278-88 pubmed publisher
    ..Our data suggest that efficient and persistent downregulation of host genes may be an important component of symptom development in certain host-virus interactions. ..
  16. Choi I, White K. An RNA activator of subgenomic mRNA1 transcription in tomato bushy stunt virus. J Biol Chem. 2002;277:3760-6 pubmed
    ..These data are most consistent with a role for this interaction in regulating sg mRNA1 accumulation at the level of transcription. ..
  17. Lin H, White K. A complex network of RNA-RNA interactions controls subgenomic mRNA transcription in a tombusvirus. EMBO J. 2004;23:3365-74 pubmed
    ..RNA components of a multipartite RNA-based control system, (ii) support a premature termination mechanism for tombusvirus sg mRNA transcription and (iii) reveal a close mechanistic relationship between sg mRNA transcription, viral ..
  18. Panavas T, Hawkins C, Panaviene Z, Nagy P. The role of the p33:p33/p92 interaction domain in RNA replication and intracellular localization of p33 and p92 proteins of Cucumber necrosis tombusvirus. Virology. 2005;338:81-95 pubmed
  19. Wu B, Pogany J, Na H, Nicholson B, Nagy P, White K. A discontinuous RNA platform mediates RNA virus replication: building an integrated model for RNA-based regulation of viral processes. PLoS Pathog. 2009;5:e1000323 pubmed publisher
    ..Here we report that a third fundamental tombusvirus process, viral genome replication, requires a long-range RNA-based interaction spanning approximately 3000 nts...
  20. Jiang Y, Li Z, Nagy P. Nucleolin/Nsr1p binds to the 3' noncoding region of the tombusvirus RNA and inhibits replication. Virology. 2010;396:10-20 pubmed publisher
    Previous genome-wide screens identified >100 host genes affecting tombusvirus replication using yeast model host...
  21. Tavazza M, Lucioli A, Calogero A, Pay A, Tavazza R. Nucleotide sequence, genomic organization and synthesis of infectious transcripts from a full-length clone of artichoke mottle crinkle virus. J Gen Virol. 1994;75 ( Pt 7):1515-24 pubmed publisher
    The complete nucleotide sequence of the genome of artichoke mottle crinkle virus (AMCV), a member of the tombusvirus group, has been determined. The genome is 4790 nucleotides (nt) in length...
  22. Lin H, Xu W, White K. A multicomponent RNA-based control system regulates subgenomic mRNA transcription in a tombusvirus. J Virol. 2007;81:2429-39 pubmed
    ..X. Lin and K. A. White, EMBO J. 23:3365-3374, 2004), the transcription of tombusvirus sg mRNAs also involves several additional RNA structures...
  23. Wang R, Stork J, Pogany J, Nagy P. A temperature sensitive mutant of heat shock protein 70 reveals an essential role during the early steps of tombusvirus replication. Virology. 2009;394:28-38 pubmed publisher
    ..Taken together, the cytosolic SSA subfamily of Hsp70 proteins play essential and multiple roles in TBSV replication. ..
  24. Everett A, Scholthof H, Scholthof K. Satellite panicum mosaic virus coat protein enhances the performance of plant virus gene vectors. Virology. 2010;396:37-46 pubmed publisher
    ..These results suggest that the multifaceted SPMV CP has intriguing effects on virus-host interactions that surface in heterologous systems...
  25. Pathak K, Pogany J, Nagy P. Non-template functions of the viral RNA in plant RNA virus replication. Curr Opin Virol. 2011;1:332-8 pubmed publisher
    ..Altogether, understanding of the non-template functions of the viral RNA during viral replication provides new insights into virus-host interactions. ..
  26. Ray D, Wu B, White K. A second functional RNA domain in the 5' UTR of the Tomato bushy stunt virus genome: intra- and interdomain interactions mediate viral RNA replication. RNA. 2003;9:1232-45 pubmed
    ..Tomato bushy stunt virus (TBSV) belongs to this class of RNA virus and is the prototype member of the genus Tombusvirus. Previous studies have demonstrated that a T-shaped domain (TSD) forms in the 5' half of the TBSV 5' UTR and ..
  27. Cheng C, Nagy P. Mechanism of RNA recombination in carmo- and tombusviruses: evidence for template switching by the RNA-dependent RNA polymerase in vitro. J Virol. 2003;77:12033-47 pubmed
    ..recombinant RdRp of Turnip crinkle carmovirus or a partially purified RdRp preparation of Cucumber necrosis tombusvirus. We demonstrated that these RdRp preparations generated RNA recombinants in vitro...
  28. Panaviene Z, Panavas T, Serva S, Nagy P. Purification of the cucumber necrosis virus replicase from yeast cells: role of coexpressed viral RNA in stimulation of replicase activity. J Virol. 2004;78:8254-63 pubmed
    ..In this work, we obtained a highly active template-dependent replicase complex for Cucumber necrosis tombusvirus (CNV), which is a plus-stranded RNA virus, from Saccharomyces cerevisiae...
  29. Cheng C, Jaag H, Jonczyk M, Serviene E, Nagy P. Expression of the Arabidopsis Xrn4p 5'-3' exoribonuclease facilitates degradation of tombusvirus RNA and promotes rapid emergence of viral variants in plants. Virology. 2007;368:238-48 pubmed
    ..A previous genome-wide screen for host factors affecting recombination in Tomato bushy stunt tombusvirus (TBSV), a small monopartite plant virus, identified Xrn1p 5'-3' exoribonuclease of yeast, a model host, whose ..
  30. Pathak K, Sasvari Z, Nagy P. The host Pex19p plays a role in peroxisomal localization of tombusvirus replication proteins. Virology. 2008;379:294-305 pubmed publisher
    ..To identify the host proteins involved in assisting the peroxisomal localization of the tombusvirus p33 replication protein, we tested if p33 could bind directly to yeast proteins involved in peroxisomal ..
  31. Sasvari Z, Izotova L, Kinzy T, Nagy P. Synergistic roles of eukaryotic translation elongation factors 1B? and 1A in stimulation of tombusvirus minus-strand synthesis. PLoS Pathog. 2011;7:e1002438 pubmed publisher
    ..eEF1B? binds to the viral RNA and is one of the resident host proteins in the tombusvirus replicase complex...
  32. Havelda Z, Hornyik C, V l czi A, Burgy n J. Defective interfering RNA hinders the activity of a tombusvirus-encoded posttranscriptional gene silencing suppressor. J Virol. 2005;79:450-7 pubmed publisher
    ..The presence of DI RNAs in tombusvirus-infected plants reduces the accumulation of helper virus RNA and results in the development of attenuated ..
  33. Monkewich S, Lin H, Fabian M, Xu W, Na H, Ray D, et al. The p92 polymerase coding region contains an internal RNA element required at an early step in Tombusvirus genome replication. J Virol. 2005;79:4848-58 pubmed
    ..Together, these results suggest that IRE activity is required in the cytosol at an early step in the viral replication process, such as template recruitment and/or replicase complex assembly. ..
  34. Szittya G, Moxon S, Pantaleo V, Toth G, Rusholme Pilcher R, Moulton V, et al. Structural and functional analysis of viral siRNAs. PLoS Pathog. 2010;6:e1000838 pubmed publisher
    ..The sequence profiles and Terminator digests suggest that CymRSV siRNAs are produced from the structured positive strand rather than from perfect double stranded RNA or by RNA dependent RNA polymerase. ..
  35. Serviene E, Jiang Y, Cheng C, Baker J, Nagy P. Screening of the yeast yTHC collection identifies essential host factors affecting tombusvirus RNA recombination. J Virol. 2006;80:1231-41 pubmed
    ..Overall, this and previous works firmly establish that a set of essential and nonessential host genes could affect TBSV recombination and evolution. ..
  36. Angel C, Hsieh Y, Schoelz J. Comparative analysis of the capacity of tombusvirus P22 and P19 proteins to function as avirulence determinants in Nicotiana species. Mol Plant Microbe Interact. 2011;24:91-9 pubmed publisher
    We have used an agroinfiltration assay for a comparative study of the roles of tombusvirus P22 and P19 proteins in elicitation of hypersensitive response (HR)-like necrosis and the role of P19 in silencing suppression in Nicotiana ..
  37. Na H, White K. Structure and prevalence of replication silencer-3' terminus RNA interactions in Tombusviridae. Virology. 2006;345:305-16 pubmed
    ..This survey also revealed a significant level of diversity and modularity with respect to RSEs, 3'CSSs and their structural contexts and, moreover, suggests that RSE-3'CSS interactions are prevalent in Tombusviridae and related viruses...
  38. Jaag H, Stork J, Nagy P. Host transcription factor Rpb11p affects tombusvirus replication and recombination via regulating the accumulation of viral replication proteins. Virology. 2007;368:388-404 pubmed publisher
    Previous genome-wide screens identified over 100 host genes whose deletion/down-regulation affected tombusvirus replication and 32 host genes that affected tombusvirus RNA recombination in yeast, a model host for replication of Tomato ..
  39. Rubino L, Russo M. Membrane targeting sequences in tombusvirus infections. Virology. 1998;252:431-7 pubmed
    ..The CymRSV 33-kDa protein did not contain the obvious consensus signals thought to be characteristic of proteins targeted to peroxisomes, and an mitochondrial targeting sequence motif was not evident. ..
  40. Wu B, Vanti W, White K. An RNA domain within the 5' untranslated region of the tomato bushy stunt virus genome modulates viral RNA replication. J Mol Biol. 2001;305:741-56 pubmed
    ..Collectively, these data support a fundamental role for the TSD, and in particular its S1 subelement, in tombusvirus RNA replication.
  41. Qu F, Morris T. Efficient infection of Nicotiana benthamiana by Tomato bushy stunt virus is facilitated by the coat protein and maintained by p19 through suppression of gene silencing. Mol Plant Microbe Interact. 2002;15:193-202 pubmed
    ..benthamiana. The CP likely enables efficient viral unloading from the vascular system in the form of assembled virions, whereas p19 enhances systemic infection by suppressing the virus-induced gene silencing. ..
  42. Serviene E, Shapka N, Cheng C, Panavas T, Phuangrat B, Baker J, et al. Genome-wide screen identifies host genes affecting viral RNA recombination. Proc Natl Acad Sci U S A. 2005;102:10545-50 pubmed
    ..Overall, our results demonstrate that a set of host genes have a major effect on RNA virus recombination and evolution. ..
  43. Jonczyk M, Pathak K, Sharma M, Nagy P. Exploiting alternative subcellular location for replication: tombusvirus replication switches to the endoplasmic reticulum in the absence of peroxisomes. Virology. 2007;362:320-30 pubmed
    ..Proc Natl Acad Sci U S A, 2005). To further test how the lack of peroxisomes could affect tombusvirus replication, we used yeast cells missing either PEX3 or PEX19 genes, which are absolutely essential for ..
  44. Ahn J, Lee J, Davarpanah S, Jeon J, Park Y, Liu J, et al. Host-dependent suppression of RNA silencing mediated by the viral suppressor p19 in potato. Planta. 2011;234:1065-72 pubmed publisher
    ..These results suggest that p19 suppresses siRNA-mediated silencing in potato, but may not affect miRNA-mediated silencing, possibly due to the host-dependent manner of p19 activity. ..
  45. Pathak K, Jiang Z, Ochanine V, Sharma M, Pogany J, Nagy P. Characterization of dominant-negative and temperature-sensitive mutants of tombusvirus replication proteins affecting replicase assembly. Virology. 2013;437:48-61 pubmed publisher
    ..the essential p33:p33/p92 interaction domain of p33 and p92 replication proteins of Cucumber necrosis virus, a tombusvirus. Mutations within the p33:p33/p92 interaction domain also affected viral RNA recombination in yeast model host...
  46. Rubino L, Di Franco A, Russo M. Expression of a plant virus non-structural protein in Saccharomyces cerevisiae causes membrane proliferation and altered mitochondrial morphology. J Gen Virol. 2000;81:279-86 pubmed
    Carnation Italian ringspot tombusvirus encodes a protein, referred to as 36K, that possesses a mitochondrial targeting signal and two transmembrane segments which are thought to anchor this protein to the outer membrane of the ..
  47. Chapman E, Prokhnevsky A, Gopinath K, Dolja V, Carrington J. Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step. Genes Dev. 2004;18:1179-86 pubmed
    ..In addition, p21 bound to synthetic miRNA/miRNA* and siRNA duplexes in vitro. We propose that several different suppressors act by distinct mechanisms to inhibit the incorporation of small RNAs into active RISCs. ..
  48. Panavas T, Nagy P. Mechanism of stimulation of plus-strand synthesis by an RNA replication enhancer in a tombusvirus. J Virol. 2005;79:9777-85 pubmed
    ..We found that the template activity of the tombusvirus replicase preparation was stimulated in trans by the REN, suggesting that the REN is a functional enhancer when ..
  49. Li Z, Pogany J, Tupman S, Esposito A, Kinzy T, Nagy P. Translation elongation factor 1A facilitates the assembly of the tombusvirus replicase and stimulates minus-strand synthesis. PLoS Pathog. 2010;6:e1001175 pubmed publisher
    ..eukaryotic translation elongation factor (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex...
  50. Huang T, Nagy P. Direct inhibition of tombusvirus plus-strand RNA synthesis by a dominant negative mutant of a host metabolic enzyme, glyceraldehyde-3-phosphate dehydrogenase, in yeast and plants. J Virol. 2011;85:9090-102 pubmed publisher
    ..Moreover, this mutant also exhibits strong suppression of tombusvirus accumulation in yeast and in virus-infected Nicotiana benthamiana...
  51. Pantaleo V, Szittya G, Burgyan J. Molecular bases of viral RNA targeting by viral small interfering RNA-programmed RISC. J Virol. 2007;81:3797-806 pubmed
    ..Here we analyzed the viral RNA targeting by VIGS in tombusvirus-infected plants, and we show evidence that antiviral response of VIGS is based on viral RNA cleavage by RNA-..
  52. Angel C, Schoelz J. A survey of resistance to Tomato bushy stunt virus in the genus Nicotiana reveals that the hypersensitive response is triggered by one of three different viral proteins. Mol Plant Microbe Interact. 2013;26:240-8 pubmed publisher
    ..tabacum), and Alatae (N. bonariensis). In general, Nicotiana spp. were capable of recognizing only one tombusvirus avirulence determinant, with the exceptions of N. bonariensis and N...
  53. Rubino L, Burgyan J, Russo M. Molecular cloning and complete nucleotide sequence of carnation Italian ringspot tombusvirus genomic and defective interfering RNAs. Arch Virol. 1995;140:2027-39 pubmed
    The complete sequences of genomic and defective interfering (DI) RNAs of carnation Italian ringspot tombusvirus (CIRV) were determined...
  54. Qiu W, Park J, Scholthof H. Tombusvirus P19-mediated suppression of virus-induced gene silencing is controlled by genetic and dosage features that influence pathogenicity. Mol Plant Microbe Interact. 2002;15:269-80 pubmed
  55. Panaviene Z, Baker J, Nagy P. The overlapping RNA-binding domains of p33 and p92 replicase proteins are essential for tombusvirus replication. Virology. 2003;308:191-205 pubmed
    ..Virol. 5845-5851), in which p92 was expressed from the genomic RNA of a tombusvirus, while p33 was expressed from a DI RNA...
  56. Fabian M, Na H, Ray D, White K. 3'-Terminal RNA secondary structures are important for accumulation of tomato bushy stunt virus DI RNAs. Virology. 2003;313:567-80 pubmed
    ..The existence of SLs 1 and 3 was also supported by comparative secondary structure analysis of sequenced tombusvirus genomes...
  57. Fabian M, White K. 5'-3' RNA-RNA interaction facilitates cap- and poly(A) tail-independent translation of tomato bushy stunt virus mrna: a potential common mechanism for tombusviridae. J Biol Chem. 2004;279:28862-72 pubmed
    Tomato bushy stunt virus (TBSV) is the prototypical member of the genus Tombusvirus in the family Tombusviridae...
  58. Omarov R, Sparks K, Smith L, Zindovic J, Scholthof H. Biological relevance of a stable biochemical interaction between the tombusvirus-encoded P19 and short interfering RNAs. J Virol. 2006;80:3000-8 pubmed publisher
    ..The findings should be particularly valuable for the RNAi field in general because the P19 mutants enable precise determination of siRNA appropriation effects...
  59. Scholthof H. The Tombusvirus-encoded P19: from irrelevance to elegance. Nat Rev Microbiol. 2006;4:405-11 pubmed
    Since its discovery in the late 1980s, the status of the Tombusvirus-encoded p19 protein (P19) changed from being thought obsolete to its identification a decade later as an important viral pathogenicity factor...
  60. Scholthof H. Rapid delivery of foreign genes into plants by direct rub-inoculation with intact plasmid DNA of a tomato bushy stunt virus gene vector. J Virol. 1999;73:7823-9 pubmed
    ..This is the first demonstration of an infectious DNA from a member of the Tombusviridae which permits rapid TBSV-mediated foreign-gene expression upon direct rub-inoculation of miniprep DNA onto a variety of plant species...
  61. Panavas T, Pogany J, Nagy P. Analysis of minimal promoter sequences for plus-strand synthesis by the Cucumber necrosis virus RNA-dependent RNA polymerase. Virology. 2002;296:263-74 pubmed
    ..Taken together, these results demonstrate that promoter recognition by the tombusvirus RdRp requires only short sequences present at the 3' end of templates.
  62. Szittya G, Silhavy D, Molnar A, Havelda Z, Lovas A, Lakatos L, et al. Low temperature inhibits RNA silencing-mediated defence by the control of siRNA generation. EMBO J. 2003;22:633-40 pubmed
    ..However, temperature does not influence the accumulation of micro (mi) RNAs, which play a role in developmental regulation, suggesting that the two classes of small (si and mi) RNAs are generated by different nuclease complexes...
  63. Pogany J, Fabian M, White K, Nagy P. A replication silencer element in a plus-strand RNA virus. EMBO J. 2003;22:5602-11 pubmed
    ..templates of tomato bushy stunt virus and a partially purified, RNA-dependent RNA polymerase preparation from tombusvirus-infected plants, revealed that this interaction inhibits minus-strand synthesis 7-fold...
  64. Lakatos L, Szittya G, Silhavy D, Burgyan J. Molecular mechanism of RNA silencing suppression mediated by p19 protein of tombusviruses. EMBO J. 2004;23:876-84 pubmed
    ..Furthermore, the obtained results also suggest that the p19-mediated sequestration of siRNAs in virus-infected cells blocks the spread of the mobile, systemic signal of RNA silencing...
  65. Navarro B, Rubino L, Russo M. Expression of the Cymbidium ringspot virus 33-kilodalton protein in Saccharomyces cerevisiae and molecular dissection of the peroxisomal targeting signal. J Virol. 2004;78:4744-52 pubmed
    ..In yeast mutants lacking peroxisomes p33 was detected in the ER. The possible involvement of the ER as an intermediate step for the integration of p33 into the peroxisomal membrane is discussed...
  66. Panavas T, Serviene E, Brasher J, Nagy P. Yeast genome-wide screen reveals dissimilar sets of host genes affecting replication of RNA viruses. Proc Natl Acad Sci U S A. 2005;102:7326-31 pubmed
    ..This finding suggests that virus infection and disease-causing proteins might use or alter similar host pathways and may suggest connections between chronic diseases and prior virus infection...
  67. Serva S, Nagy P. Proteomics analysis of the tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication. J Virol. 2006;80:2162-9 pubmed
    ..To identify host proteins present in the cucumber necrosis tombusvirus (CNV) replicase, we affinity purified functional viral replicase complexes from yeast...
  68. Hsieh Y, Omarov R, Scholthof H. Diverse and newly recognized effects associated with short interfering RNA binding site modifications on the Tomato bushy stunt virus p19 silencing suppressor. J Virol. 2009;83:2188-200 pubmed publisher
    ..benthamiana plants but not in spinach. In conclusion, even though siRNA binding by P19 is a key functional property, compromised siRNA sequestration can result in novel and diverse host-dependent properties...
  69. Nagy P. The roles of host factors in tombusvirus RNA recombination. Adv Virus Res. 2011;81:63-84 pubmed publisher
    ..These advances will help build more accurate models on viral recombination, evolution, and adaptation...
  70. Cheng C, Pogany J, Nagy P. Mechanism of DI RNA formation in tombusviruses: dissecting the requirement for primer extension by the tombusvirus RNA dependent RNA polymerase in vitro. Virology. 2002;304:460-73 pubmed
    ..This model was tested using a partially purified RNA-dependent RNA polymerase (RdRp) preparation from tombusvirus-infected plants in vitro...
  71. Rajendran K, Nagy P. Characterization of the RNA-binding domains in the replicase proteins of tomato bushy stunt virus. J Virol. 2003;77:9244-58 pubmed
    Tomato bushy stunt virus (TBSV), a tombusvirus with a nonsegmented, plus-stranded RNA genome, codes for two essential replicase proteins...
  72. Omarov R, Rezende J, Scholthof H. Host-specific generation and maintenance of Tomato bushy stunt virus defective interfering RNAs. Mol Plant Microbe Interact. 2004;17:195-201 pubmed
    ..benthamiana did not yield DI in upper pepper leaves. Our cumulative results suggest that complex host-specific determinants play an important role in TBSV DI generation and their subsequent maintenance and accumulation...
  73. Roth B, Pruss G, Vance V. Plant viral suppressors of RNA silencing. Virus Res. 2004;102:97-108 pubmed
    ..Because they interfere with silencing at different points in the pathway, these viral suppressors are powerful tools to help unravel the mechanism of RNA silencing in plants...
  74. Cheng C, Panavas T, Luo G, Nagy P. Heterologous RNA replication enhancer stimulates in vitro RNA synthesis and template-switching by the carmovirus, but not by the tombusvirus, RNA-dependent RNA polymerase: implication for modular evolution of RNA viruses. Virology. 2005;341:107-21 pubmed
    ..On the contrary, RNA synthesis by the TCV RdRp was stimulated by the TCV REN and the heterologous tombusvirus REN with comparable efficiency...
  75. Stork J, Panaviene Z, Nagy P. Inhibition of in vitro RNA binding and replicase activity by phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus. Virology. 2005;343:79-92 pubmed
    ..Stork, J., Nagy, P.D., 2005. Phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus adjacent to the RNA binding site affects viral RNA replication. J. Virol. 343, 65-78...
  76. Li Z, Pogany J, Panavas T, Xu K, Esposito A, Kinzy T, et al. Translation elongation factor 1A is a component of the tombusvirus replicase complex and affects the stability of the p33 replication co-factor. Virology. 2009;385:245-60 pubmed publisher TBSV RNA, namely translation elongation factor eEF1A, revealed that it is present in the highly purified tombusvirus replicase complex...
  77. Barajas D, Jiang Y, Nagy P. A unique role for the host ESCRT proteins in replication of Tomato bushy stunt virus. PLoS Pathog. 2009;5:e1000705 pubmed publisher
    ..Previous genome-wide screens with Tomato bushy stunt tombusvirus (TBSV) in a yeast model host revealed the involvement of seven ESCRT (endosomal sorting complexes required for ..
  78. Várallyay E, Valoczi A, Agyi A, Burgyan J, Havelda Z. Plant virus-mediated induction of miR168 is associated with repression of ARGONAUTE1 accumulation. EMBO J. 2010;29:3507-19 pubmed publisher
    ..the induction of miR168, which overlaps spatially with virus-occupied sectors, is mediated mainly by the Tombusvirus p19 RNA-silencing suppressor...
  79. Shah Nawaz ul Rehman M, Martinez Ochoa N, Pascal H, Sasvari Z, Herbst C, Xu K, et al. Proteome-wide overexpression of host proteins for identification of factors affecting tombusvirus RNA replication: an inhibitory role of protein kinase C. J Virol. 2012;86:9384-95 pubmed publisher
  80. Nagy P, Pogany J. Partial purification and characterization of Cucumber necrosis virus and Tomato bushy stunt virus RNA-dependent RNA polymerases: similarities and differences in template usage between tombusvirus and carmovirus RNA-dependent RNA polymerases. Virology. 2000;276:279-88 pubmed
    ..of de novo initiation of complementary RNA synthesis using either plus- or minus-strand templates derived from tombusvirus defective interfering (DI) RNAs...
  81. Koenig R, Verhoeven J, Fribourg C, Pfeilstetter E, Lesemann D. Evaluation of various species demarcation criteria in attempts to classify ten new tombusvirus isolates. Arch Virol. 2004;149:1733-44 pubmed
    ..suggested species demarcation criteria was compared in attempts to determine the taxonomic status of ten new tombusvirus isolates...
  82. Pogany J, White K, Nagy P. Specific binding of tombusvirus replication protein p33 to an internal replication element in the viral RNA is essential for replication. J Virol. 2005;79:4859-69 pubmed
    ..Using the prototypical tombusvirus, Tomato bushy stunt virus (TBSV), we show that recombinant p33 replicase protein binds specifically to an ..
  83. Panaviene Z, Panavas T, Nagy P. Role of an internal and two 3'-terminal RNA elements in assembly of tombusvirus replicase. J Virol. 2005;79:10608-18 pubmed
    ..The replicase of Cucumber necrosis virus (CNV), a tombusvirus, contains the viral p33 and p92 replication proteins and possible host factors...
  84. Rajendran K, Nagy P. Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: requirement of p33:p92 interaction for replicase assembly. Virology. 2006;345:270-9 pubmed publisher
    ..essential interactions between p33:p33 and p33:p92 replication proteins of Tomato bushy stunt virus (TBSV), a tombusvirus with a non-segmented, plus-stranded RNA genome...
  85. Wang R, Stork J, Nagy P. A key role for heat shock protein 70 in the localization and insertion of tombusvirus replication proteins to intracellular membranes. J Virol. 2009;83:3276-87 pubmed publisher
    ..Tomato bushy stunt tombusvirus (TBSV) is a model virus that can replicate a small replicon RNA in Saccharomyces cerevisiae and in plants...
  86. Sasvari Z, Kovalev N, Nagy P. The GEF1 proton-chloride exchanger affects tombusvirus replication via regulation of copper metabolism in yeast. J Virol. 2013;87:1800-10 pubmed publisher
    ..We also show that the in vitro activity of the purified tombusvirus replicase from gef1? yeast was low and that the in vitro assembly of the viral replicase in a cell extract was ..
  87. Baulcombe D, Molnar A. Crystal structure of p19--a universal suppressor of RNA silencing. Trends Biochem Sci. 2004;29:279-81 pubmed
    ..The recently reported crystal structure of two Tombusvirus suppressor proteins reveals a novel RNA-binding structure and illustrates precisely how the silencing mechanism ..
  88. Wu B, White K. Uncoupling RNA virus replication from transcription via the polymerase: functional and evolutionary insights. EMBO J. 2007;26:5120-30 pubmed
    ..Here we show that tombusvirus genome replication can be effectively uncoupled from sg mRNA transcription in vivo by C-terminal modifications ..
  89. Wang R, Nagy P. Tomato bushy stunt virus co-opts the RNA-binding function of a host metabolic enzyme for viral genomic RNA synthesis. Cell Host Microbe. 2008;3:178-87 pubmed publisher
    ..Downregulation of GAPDH in a natural plant host decreased TBSV genomic RNA accumulation. Thus, TBSV co-opts the RNA-binding function of a metabolic protein, helping convert the host cell into a viral factory...
  90. Jaag H, Nagy P. Silencing of Nicotiana benthamiana Xrn4p exoribonuclease promotes tombusvirus RNA accumulation and recombination. Virology. 2009;386:344-52 pubmed publisher
    ..5'-to-3' exoribonuclease Xrn1p plays a major role in recombination and degradation of Tomato bushy stunt tombusvirus (TBSV) replicon (rep)RNA in yeast, a model host (Serviene, E., Shapka, N., Cheng, C.P., Panavas, T...
  91. Jaag H, Pogany J, Nagy P. A host Ca2+/Mn2+ ion pump is a factor in the emergence of viral RNA recombinants. Cell Host Microbe. 2010;7:74-81 pubmed publisher
    ..Thus, a conserved host protein could affect the adaptive evolution of RNA viruses...
  92. Stork J, Kovalev N, Sasvari Z, Nagy P. RNA chaperone activity of the tombusviral p33 replication protein facilitates initiation of RNA synthesis by the viral RdRp in vitro. Virology. 2011;409:338-47 pubmed publisher
    ..Altogether, the TBSV RNA chaperone might perform similar biological functions to the helicases of other RNA viruses with much larger coding capacity...