Summary: A genus of plant viruses, in the family TYMOVIRIDAE, possessing a narrow host range that includes CRUCIFERAE. Transmission occurs by BEETLES and mechanical inoculation.

Top Publications

  1. Prod homme D, Le Panse S, Drugeon G, Jupin I. Detection and subcellular localization of the turnip yellow mosaic virus 66K replication protein in infected cells. Virology. 2001;281:88-101 pubmed
    ..Indirect immunofluorescence assays and electron microscopy of immunogold-decorated ultrathin sections of infected leaf tissue using anti-66K-specific antibody revealed labeling of membrane vesicles located at the chloroplast envelope...
  2. van Roon A, Bink H, Plaisier J, Pleij C, Abrahams J, Pannu N. Crystal structure of an empty capsid of turnip yellow mosaic virus. J Mol Biol. 2004;341:1205-14 pubmed
    ..Further differences were found at the N terminus of the A-subunit. These differences have implications for the mechanism of decapsidation of the virus, required for infection...
  3. Hammond J, Rambo R, Kieft J. Multi-domain packing in the aminoacylatable 3' end of a plant viral RNA. J Mol Biol. 2010;399:450-63 pubmed publisher
    ..The TYMV TLS.UPD might demonstrate how RNA structural plasticity can play a role in regulation of biological processes...
  4. Wientges J, Putz J, Giege R, Florentz C, Schwienhorst A. Selection of viral RNA-derived tRNA-like structures with improved valylation activities. Biochemistry. 2000;39:6207-18 pubmed
    ..This is in line with the role of the TLS in viral replication...
  5. Jakubiec A, Notaise J, Tournier V, Héricourt F, Block M, Drugeon G, et al. Assembly of turnip yellow mosaic virus replication complexes: interaction between the proteinase and polymerase domains of the replication proteins. J Virol. 2004;78:7945-57 pubmed
    ..These results support a novel model for the interaction of TYMV replication proteins and suggest that viruses in the alphavirus-like supergroup may have selected different pathways to assemble their replication complexes...
  6. Segwagwe A, Putnam M, Druffel K, Pappu H, Eastwell K. Molecular characterization of a new tymovirus from Diascia ornamental plants. Arch Virol. 2008;153:1495-503 pubmed publisher
    ..Sequence analysis also revealed the presence of a new tymovirus, and the entire genomic sequence of this virus was determined...
  7. Pag n I, Betancourt M, de Miguel J, Pi ero D, Fraile A, Garc a Arenal F. Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico. Arch Virol. 2010;155:675-84 pubmed publisher
    ..In this work, the complete genomic nucleotide sequence of a new tymovirus species infecting chiltepin, the wild ancestor of Capsicum annuum pepper crops, in Mexico was determined, and ..
  8. Camborde L, Planchais S, Tournier V, Jakubiec A, Drugeon G, Lacassagne E, et al. The ubiquitin-proteasome system regulates the accumulation of Turnip yellow mosaic virus RNA-dependent RNA polymerase during viral infection. Plant Cell. 2010;22:3142-52 pubmed publisher
  9. Schirawski J, Planchais S, Haenni A. An improved protocol for the preparation of protoplasts from an established Arabidopsis thaliana cell suspension culture and infection with RNA of turnip yellow mosaic tymovirus: a simple and reliable method. J Virol Methods. 2000;86:85-94 pubmed
    ..These protoplasts can be transformed efficiently with RNA from turnip yellow mosaic tymovirus (TYMV) by polyethyleneglycol-mediated transfection...

More Information

Publications104 found, 100 shown here

  1. Schirawski J, Voyatzakis A, Zaccomer B, Bernardi F, Haenni A. Identification and functional analysis of the turnip yellow mosaic tymovirus subgenomic promoter. J Virol. 2000;74:11073-80 pubmed
    ..Turnip yellow mosaic tymovirus (TYMV) is a positive-stranded RNA virus that produces an sgRNA for the expression of its coat protein...
  2. Hema M, Nagendrakumar S, Yamini R, Chandran D, Rajendra L, Thiagarajan D, et al. Chimeric tymovirus-like particles displaying foot-and-mouth disease virus non-structural protein epitopes and its use for detection of FMDV-NSP antibodies. Vaccine. 2007;25:4784-94 pubmed
    Expression of Physalis mottle tymovirus (PhMV) coat protein (CP) in Escherichia coli (E. coli) was earlier shown to self-assemble into empty capsids that are nearly identical to the capsids formed in vivo...
  3. Min B, Feldman T, Ali A, Wiley G, Muthukumar V, Roe B, et al. Molecular characterization, ecology, and epidemiology of a novel Tymovirus in Asclepias viridis from Oklahoma. Phytopathology. 2012;102:166-76 pubmed publisher
    ..A novel tymovirus sequence was detected in Asclepias viridis (green milkweed), a perennial growing in a natural setting in the ..
  4. Barends S, Bink H, van den Worm S, Pleij C, Kraal B. Entrapping ribosomes for viral translation: tRNA mimicry as a molecular Trojan horse. Cell. 2003;112:123-9 pubmed
    ..Disruption of the TLS exclusively abolishes polyprotein synthesis, which can be restored by adding excess TLS in trans. Our observations imply a novel eukaryotic mechanism for internal initiation of mRNA translation...
  5. Prod homme D, Jakubiec A, Tournier V, Drugeon G, Jupin I. Targeting of the turnip yellow mosaic virus 66K replication protein to the chloroplast envelope is mediated by the 140K protein. J Virol. 2003;77:9124-35 pubmed
    ..These results suggests that the 140K protein is a key organizer of the assembly of the TYMV replication complexes and a major determinant for their chloroplastic localization and retention...
  6. Jakubiec A, Drugeon G, Camborde L, Jupin I. Proteolytic processing of turnip yellow mosaic virus replication proteins and functional impact on infectivity. J Virol. 2007;81:11402-12 pubmed
    ..We present evidence that PRO/HEL cleavage is critical for productive virus infection and that the impaired infectivity of PRO/HEL cleavage mutants is due mainly to defective synthesis of positive-strand RNA...
  7. Tzanetakis I, Tsai C, Martin R, Dreher T. A tymovirus with an atypical 3'-UTR illuminates the possibilities for 3'-UTR evolution. Virology. 2009;392:238-45 pubmed publisher
    We report the complete genome sequence of Dulcamara mottle virus (DuMV), confirming its membership within the Tymovirus genus, which was previously based on physical and pathology evidence...
  8. Chenon M, Camborde L, Cheminant S, Jupin I. A viral deubiquitylating enzyme targets viral RNA-dependent RNA polymerase and affects viral infectivity. EMBO J. 2012;31:741-53 pubmed publisher
  9. Dreher T, Goodwin J. Transfer RNA mimicry among tymoviral genomic RNAs ranges from highly efficient to vestigial. Nucleic Acids Res. 1998;26:4356-64 pubmed
    ..The range of tRNA mimicry within the tymovirus genus thus ranges from extremely highly developed to minimal...
  10. Matsuda D, Dreher T. The tRNA-like structure of Turnip yellow mosaic virus RNA is a 3'-translational enhancer. Virology. 2004;321:36-46 pubmed
    ..The UPSK seems to act as an important, though nonspecific, spacer element ensuring proper presentation of a functional TLS. Our studies have uncovered a novel type of translational enhancer and a new role for a plant viral TLS...
  11. Matsuda D, Yoshinari S, Dreher T. eEF1A binding to aminoacylated viral RNA represses minus strand synthesis by TYMV RNA-dependent RNA polymerase. Virology. 2004;321:47-56 pubmed
    ..It is proposed that minus strand repression by eEF1A.GTP binding occurs early during infection to help coordinate the competing translation and replication functions of the genomic RNA...
  12. Koenig R, Barends S, Gultyaev A, Lesemann D, Vetten H, Loss S, et al. Nemesia ring necrosis virus: a new tymovirus with a genomic RNA having a histidylatable tobamovirus-like 3' end. J Gen Virol. 2005;86:1827-33 pubmed publisher
    ..folding properties of its 5'-untranslated region and in vitro translation experiments, NeRNV RNA is a typical tymovirus RNA...
  13. Niu Q, Lin S, Reyes J, Chen K, Wu H, Yeh S, et al. Expression of artificial microRNAs in transgenic Arabidopsis thaliana confers virus resistance. Nat Biotechnol. 2006;24:1420-8 pubmed
    ..The amiRNA-mediated approach should have broad applicability for engineering multiple virus resistance in crop plants...
  14. Bink H, Pleij C. RNA-protein interactions in spherical viruses. Arch Virol. 2002;147:2261-79 pubmed
    ..It is only in the case of the RNA bacteriophages that we know in atomic detail how an RNA hairpin interacts with the coat protein. ..
  15. Shin H, Kim H, Cho T. The Pro/Hel region is indispensable for packaging non-replicating turnip yellow mosaic virus RNA, but not replicating viral RNA. Mol Cells. 2010;29:463-9 pubmed publisher
  16. Koenig R, Pleij C, Lesemann D, Loss S, Vetten H. Molecular characterization of isolates of anagyris vein yellowing virus, plantago mottle virus and scrophularia mottle virus -- comparison of various approaches for tymovirus classification. Arch Virol. 2005;150:2325-38 pubmed
    ..Total nucleotide and coat protein amino acid sequence identities revealed similar groupings in the genus tymovirus as serological studies did...
  17. Robin C, Beaurepaire L, Chenon M, Jupin I, Bressanelli S. In praise of impurity: 30S ribosomal S15 protein-assisted crystallization of turnip yellow mosaic virus proteinase. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012;68:486-90 pubmed publisher
  18. Stephan D, Siddiqua M, Ta Hoang A, Engelmann J, Winter S, Maiss E. Complete nucleotide sequence and experimental host range of Okra mosaic virus. Virus Genes. 2008;36:231-40 pubmed publisher
    Okra mosaic virus (OkMV) is a tymovirus infecting members of the family Malvaceae. Early infections in okra (Abelmoschus esculentus) lead to yield losses of 12-19.5%...
  19. Pagán I, González Jara P, Moreno Letelier A, Rodelo Urrego M, Fraile A, Piñero D, et al. Effect of biodiversity changes in disease risk: exploring disease emergence in a plant-virus system. PLoS Pathog. 2012;8:e1002796 pubmed publisher
    ..These results are relevant for managing plant diseases and for establishing conservation policies for endangered plant species. ..
  20. Lacerda A, Fonseca L, Blawid R, Boiteux L, Ribeiro S, Brasileiro A. Reference Gene Selection for qPCR Analysis in Tomato-Bipartite Begomovirus Interaction and Validation in Additional Tomato-Virus Pathosystems. PLoS ONE. 2015;10:e0136820 pubmed publisher
    ..samples, corresponding to additional three tomato-virus pathosystems that included tospovirus, crinivirus and tymovirus + tobamovirus, was analyzed together with the tomato-ToCMoV pathosystem dataset, using the same algorithms...
  21. Lin S, Wu H, Elena S, Chen K, Niu Q, Yeh S, et al. Molecular evolution of a viral non-coding sequence under the selective pressure of amiRNA-mediated silencing. PLoS Pathog. 2009;5:e1000312 pubmed publisher
    ..The pattern of substitutions within the viral target was largely dominated by G to A and C to U transitions. ..
  22. Umashankar M, Murthy M, Savithri H. Mutation of interfacial residues disrupts subunit folding and particle assembly of Physalis mottle tymovirus. J Biol Chem. 2003;278:6145-52 pubmed
    ..To gain insights into the assembly mechanisms of the Physalis mottle tymovirus (PhMV), six interfacial residues, identified based on the crystal structure of the native and recombinant ..
  23. Petrzik K. Occurrence of Potato virus X on hybrid dock in Czech Republic. Acta Virol. 2009;53:49-52 pubmed
    ..Both viruses were detected 9 months after inoculation in the infected plants. ..
  24. Bink H, Schirawski J, Haenni A, Pleij C. The 5'-proximal hairpin of turnip yellow mosaic virus RNA: its role in translation and encapsidation. J Virol. 2003;77:7452-8 pubmed
    ..Slightly acidic conditions can be found in chloroplasts, where TYMV assembly is driven by a low pH generated by active photosynthesis. ..
  25. Miyoshi H, Suehiro N, Tomoo K, Muto S, Takahashi T, Tsukamoto T, et al. Binding analyses for the interaction between plant virus genome-linked protein (VPg) and plant translational initiation factors. Biochimie. 2006;88:329-40 pubmed
    ..Further experiments with affinity chromatography showed that VPg forms a ternary complex with eIF(iso)4E and eIF(iso)4G. Thus, VPg may participate in viral translational initiation by functioning as an alternative cap-like structure. ..
  26. Camborde L, Tournier V, Noizet M, Jupin I. A Turnip yellow mosaic virus infection system in Arabidopsis suspension cell culture. FEBS Lett. 2007;581:337-41 pubmed
    ..The Arabidopsis cell culture yielded soluble, active replication complexes, providing a useful tool to study host factors involved in TYMV replication. ..
  27. Zhang Y, Wang Y, Kanyuka K, Parry M, Powers S, Halford N. GCN2-dependent phosphorylation of eukaryotic translation initiation factor-2alpha in Arabidopsis. J Exp Bot. 2008;59:3131-41 pubmed publisher
    ..Analysis of wild-type and GT8359 plants infected with Turnip yellow mosaic virus or Turnip crinkle virus showed that AteIF2alpha was not phosphorylated. ..
  28. Ranjith Kumar C, Gopinath K, Jacob A, Srividhya V, Elango P, Savithri H. Genomic sequence of physalis mottle virus and its evolutionary relationship with other tymoviruses. Arch Virol. 1998;143:1489-500 pubmed
    The genome of physalis mottle tymovirus (PhMV) is 6673 nucleotides long and is rich in cytosine residues (40.58%) like other tymoviruses...
  29. Powell J, Barbar E, Dreher T. Turnip yellow mosaic virus forms infectious particles without the native beta-annulus structure and flexible coat protein N-terminus. Virology. 2012;422:165-73 pubmed publisher
  30. Malkin A, Kuznetsov Y, Lucas R, McPherson A. Surface processes in the crystallization of turnip yellow mosaic virus visualized by atomic force microscopy. J Struct Biol. 1999;127:35-43 pubmed
    ..We believe this provides the first visual record of a cooperative restructuring of the surface of a supramolecular crystal. ..
  31. Rohozinski J, Hancock J. Do light-induced pH changes within the chloroplast drive turnip yellow mosaic virus assembly?. J Gen Virol. 1996;77 ( Pt 2 ):163-5 pubmed
    ..In a low-pH environment, poly(C) regions within the genomic RNA of TYMV may interact to form tertiary structures, and the recognition of these structures by TYMV coat protein initiates virion assembly. ..
  32. Bink H, Hellendoorn K, van der Meulen J, Pleij C. Protonation of non-Watson-Crick base pairs and encapsidation of turnip yellow mosaic virus RNA. Proc Natl Acad Sci U S A. 2002;99:13465-70 pubmed
    ..5) in the presence of spermidine. Based on these results we propose that this binding of protonated cytosines to the coat protein of turnip yellow mosaic virus may represent a new motif in RNA-protein interactions. ..
  33. Dufresne P, Thivierge K, Cotton S, Beauchemin C, Ide C, Ubalijoro E, et al. Heat shock 70 protein interaction with Turnip mosaic virus RNA-dependent RNA polymerase within virus-induced membrane vesicles. Virology. 2008;374:217-27 pubmed publisher
    ..Thus, Hsc70-3 and PABP2 are potentially integral components of the replicase complex and could have important roles to play in the regulation of potyviral RdRp functions...
  34. Murphy A, Otto B, Brearley C, Carr J, Hanke D. A role for inositol hexakisphosphate in the maintenance of basal resistance to plant pathogens. Plant J. 2008;56:638-52 pubmed publisher
    ..In contrast, mutant atips1 plants, which were also depleted in InsP6, were not compromised in resistance to pathogens, suggesting that a specific pool of InsP6 regulates defence against phytopathogens. ..
  35. Joshi R, Ravel J, Haenni A. Interaction of turnip yellow mosaic virus Val-RNA with eukaryotic elongation factor EF-1 [alpha]. Search for a function. EMBO J. 1986;5:1143-8 pubmed
    ..This suggests that interaction of TYMV RNA with EF-1alpha is not mandatory for replicase activity. ..
  36. Cho T, Dreher T. Encapsidation of genomic but not subgenomic Turnip yellow mosaic virus RNA by coat protein provided in trans. Virology. 2006;356:126-35 pubmed
    ..Our results indicate differences in the encapsidation process between genomic and subgenomic RNAs, and suggest an interaction between RNA replication and the packaging of subgenomic RNA. ..
  37. Kim B, Suehiro N, Natsuaki T, Inukai T, Masuta C. The P3 protein of turnip mosaic virus can alone induce hypersensitive response-like cell death in Arabidopsis thaliana carrying TuNI. Mol Plant Microbe Interact. 2010;23:144-52 pubmed publisher
    ..In this study, we successfully demonstrated that the systemic necrosis by TuMV in Arabidopsis was determined by the gene-for-gene interaction between TuNI and P3 using the protoplast system for direct verification. ..
  38. Moeller J, Moscou M, Bancroft T, Skadsen R, Wise R, Whitham S. Differential accumulation of host mRNAs on polyribosomes during obligate pathogen-plant interactions. Mol Biosyst. 2012;8:2153-65 pubmed publisher
  39. Bradel B, Preil W, Jeske H. Sequence analysis and genome organisation of poinsettia mosaic virus (PnMV) reveal closer relationship to marafiviruses than to tymoviruses. Virology. 2000;271:289-97 pubmed publisher
    ..221-kDa polyprotein that lacks a stop codon between the replicase and the coat protein genes, as in most tymovirus RNAs...
  40. Shin H, Kim I, Cho T. Replication and encapsidation of recombinant Turnip yellow mosaic virus RNA. BMB Rep. 2008;41:739-44 pubmed
    ..An RNase protection assay revealed that the recombinant subgenomic RNA was encapsidated as efficiently as the genuine subgenomic RNA. ..
  41. Krishna S, Sastri M, Savithri H, Murthy M. Structural studies on the empty capsids of Physalis mottle virus. J Mol Biol. 2001;307:1035-47 pubmed
    The three-dimensional crystal structure of the empty capsid of Physalis mottle tymovirus has been determined to 3.2 A resolution. The empty capsids crystallized in the space group P1, leading to 60-fold non-crystallographic redundancy...
  42. Mitchell E, Bond J. Variation in the coat protein sequence of British isolates of Turnip yellow mosaic virus and comparison with previously published isolates. Brief report. Arch Virol. 2005;150:2347-55 pubmed
    ..705-0.819, mean 0.742). Comparison with other isolates showed large genetic distances between the Dorset isolates and both European and Australian isolates. ..
  43. Beauchemin C, Laliberté J. The poly(A) binding protein is internalized in virus-induced vesicles or redistributed to the nucleolus during turnip mosaic virus infection. J Virol. 2007;81:10905-13 pubmed
    ..This redistribution of an important translation initiation factor to the nucleolus and to membrane structure likely underlies two important processes of the TuMV replication cycle. ..
  44. Khan M, Goss D. Poly(A)-binding protein increases the binding affinity and kinetic rates of interaction of viral protein linked to genome with translation initiation factors eIFiso4F and eIFiso4F·4B complex. Biochemistry. 2012;51:1388-95 pubmed publisher
    ..0 ± 3.0 to 44.0 ± 2.4 kJ/mol. This suggests that the presence of both proteins leads to a rapid, stable complex, which serves to sequester initiation factors. ..
  45. Vargas Asencio J, Wojciechowska K, Baskerville M, Gomez A, Perry K, Thompson J. The complete nucleotide sequence and genomic characterization of grapevine asteroid mosaic associated virus. Virus Res. 2017;227:82-87 pubmed publisher
    ..BLAST analysis of the predicted GAMaV OP showed a unique relatedness to the OPs of members of the genus Tymovirus.
  46. Jakubiec A, Tournier V, Drugeon G, Pflieger S, Camborde L, Vinh J, et al. Phosphorylation of viral RNA-dependent RNA polymerase and its role in replication of a plus-strand RNA virus. J Biol Chem. 2006;281:21236-49 pubmed
    ..Based on these data, we propose that phosphorylation-dependent regulatory mechanisms are essential for viral RdRp function and virus replication. ..
  47. Spak J, Holy A, Pavingerová D, Votruba I, Spaková V, Petrzik K. New in vitro method for evaluating antiviral activity of acyclic nucleoside phosphonates against plant viruses. Antiviral Res. 2010;88:296-303 pubmed publisher
    ..The method enables large-scale screening of potential antivirals for in vitro elimination of viruses from vegetatively propagated crops and ornamentals. ..
  48. Domashevskiy A, Miyoshi H, Goss D. Inhibition of pokeweed antiviral protein (PAP) by turnip mosaic virus genome-linked protein (VPg). J Biol Chem. 2012;287:29729-38 pubmed publisher
    ..VPg may confer an evolutionary advantage by suppressing one of the plant defense mechanisms and also suggests the possible use of this protein against the cytotoxic activity of ribosome-inactivating proteins...
  49. Jupin I. A protocol for VIGS in Arabidopsis thaliana using a one-step TYMV-derived vector. Methods Mol Biol. 2013;975:197-210 pubmed publisher
    ..Another important characteristic of this viral vector is its capacity to infect and induce silencing in meristem tissues. ..
  50. Drugeon G, Jupin I. Stability in vitro of the 69K movement protein of Turnip yellow mosaic virus is regulated by the ubiquitin-mediated proteasome pathway. J Gen Virol. 2002;83:3187-97 pubmed
    ..We propose that selective degradation of virus movement proteins may contribute to the previously reported transient nature of their accumulation during infection. ..
  51. Sukhacheva E, Fomicheva V, Efimova N, Shubert J. [Immunodetection of RNA-dependent RNA polymerase from turnip yellow luteovirus using monoclonal antibodies]. Bioorg Khim. 2004;30:25-9 pubmed
    ..The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru. ..
  52. Lombardi C, Ayach M, Beaurepaire L, Chenon M, Andreani J, Guerois R, et al. A compact viral processing proteinase/ubiquitin hydrolase from the OTU family. PLoS Pathog. 2013;9:e1003560 pubmed publisher
    ..Our results thus shed light on how such a compact protein achieves a diversity of key functions in viral genome replication and host-pathogen interaction. ..
  53. Blawid R, Hayashi E, Rezende J, Kitajima E, Nagata T. A highly divergent isolate of tomato blistering mosaic virus from Solanum violaefolium. Virus Genes. 2016;52:294-8 pubmed publisher
    The complete genome of a tymovirus infecting Solanum violaefolium was sequenced. The genome comprised 6284 nt, with a 5'-UTR of 137 nt and a comparatively longer 3'-UTR of 121 nt...
  54. Zeng Q, Saha S, Lee L, Barnhill H, Oxsher J, Dreher T, et al. Chemoselective modification of turnip yellow mosaic virus by Cu(I) catalyzed azide-alkyne 1,3-dipolar cycloaddition reaction and its application in cell binding. Bioconjug Chem. 2011;22:58-66 pubmed publisher
    ..This illustrates the potential application of plant virus based materials in tissue engineering, drug delivery, and biosensing. ..
  55. Kreuze J, Koenig R, De Souza J, Vetten H, Muller G, Flores B, et al. The complete genome sequences of a Peruvian and a Colombian isolate of Andean potato latent virus and partial sequences of further isolates suggest the existence of two distinct potato-infecting tymovirus species. Virus Res. 2013;173:431-5 pubmed publisher
    The complete genomic RNA sequences of the tymovirus isolates Hu and Col from potato which originally had been considered to be strains of the same virus species, i.e...
  56. Edwards M, Zhang Z, Weiland J. Oat blue dwarf marafivirus resembles the tymoviruses in sequence, genome organization, and expression strategy. Virology. 1997;232:217-29 pubmed
    ..Due to the strong similarities in genome sequence and expression strategy, OBDV, and presumably the other marafiviruses, should be considered a member of the tymovirus lineage of the alpha-like plant viruses.
  57. Larson S, Lucas R, Greenwood A, McPherson A. The RNA of turnip yellow mosaic virus exhibits icosahedral order. Virology. 2005;334:245-54 pubmed
    ..The distribution of RNA observed crystallographically appears to be in agreement with models based on biochemical data and secondary structural analyses...
  58. Kuznetsov Y, McPherson A. Atomic force microscopy investigation of Turnip Yellow Mosaic Virus capsid disruption and RNA extrusion. Virology. 2006;352:329-37 pubmed
    ..Similar products were also obtained by heating TYMV to 70 -75 degrees C and by exposure to alkaline pH. Experiments showed the nucleic acid to have an elaborate secondary structure distributed linearly along its length...
  59. Barnhill H, Reuther R, Ferguson P, Dreher T, Wang Q. Turnip yellow mosaic virus as a chemoaddressable bionanoparticle. Bioconjug Chem. 2007;18:852-9 pubmed
    ..Therefore, it can potentially be employed as a reactive scaffold for the display of a variety of materials for applications in many areas of nanoscience...
  60. Grover V, Pierce M, Hoyt P, Zhang F, Melcher U. Oligonucleotide-based microarray for detection of plant viruses employing sequence-independent amplification of targets. J Virol Methods. 2010;163:57-67 pubmed publisher
    ..The system was validated in pure and mixed samples by detection of three Tymovirus species: Asclepias asymptomatic virus, Kennedya yellow mosaic virus and Turnip yellow mosaic virus...
  61. Curtin S, Zsögön A, Watson J, Waterhouse P. Isolation and analysis of small RNAs from virus-infected plants. Methods Mol Biol. 2012;894:173-89 pubmed publisher
    ..EMBO Rep 7:1168-1175, 2006; Curtin et al. FEBS Lett 582:2753-2760, 2008). The protocols presented here can also be employed for the isolation of non-virus related small RNAs such as microRNAs (miRNAs) and hairpin RNA (hpRNA)...
  62. Cumbo A, Lorber B, Corvini P, Meier W, Shahgaldian P. A synthetic nanomaterial for virus recognition produced by surface imprinting. Nat Commun. 2013;4:1503 pubmed publisher
    ..We demonstrate the formation of a chemical imprint, comparable to the formation of biosilica, due to the template effect of the virion surface on the synthesis of the recognition material...
  63. Sánchez F, Sáez M, Lunello P, Ponz F. Plant viral elongated nanoparticles modified for log-increases of foreign peptide immunogenicity and specific antibody detection. J Biotechnol. 2013;168:409-15 pubmed publisher
    ..Purified VER3 nanoparticles also showed log increases in their ability to detect VER3 antibodies in sera, when used as reagents in ELISA assays, an application also used here for the first time. ..
  64. Agbeci M, Grangeon R, Nelson R, Zheng H, Laliberté J. Contribution of host intracellular transport machineries to intercellular movement of turnip mosaic virus. PLoS Pathog. 2013;9:e1003683 pubmed publisher
    ..Using this dual gene cassette expression system and transport inhibitors, components of the secretory and actomyosin machinery were shown to be important for TuMV intercellular spread...
  65. Suehiro N, Natsuaki T, Watanabe T, Okuda S. An important determinant of the ability of Turnip mosaic virus to infect Brassica spp. and/or Raphanus sativus is in its P3 protein. J Gen Virol. 2004;85:2087-98 pubmed
    ..These results suggest that the P3 protein is an important factor in the infection cycle of TuMV and in determining the host range of this and perhaps other potyviruses...
  66. Umashankar M, Murthy M, Singh S, Appu Rao A, Savithri H. The role of inter-subunit ionic interactions in the assembly of Physalis mottle tymovirus. Arch Virol. 2006;151:1917-31 pubmed
    Physalis mottle tymovirus (PhMV) is a small spherical plant virus with its RNA genome encapsidated in a protein shell made of 180 identical coat protein (CP) subunits...
  67. Garcia J, Simón Mateo C. A micropunch against plant viruses. Nat Biotechnol. 2006;24:1358-9 pubmed
  68. Shin H, Cho N, Cho T. Role of 5'-UTR hairpins of the Turnip yellow mosaic virus RNA in replication and systemic movement. BMB Rep. 2008;41:778-83 pubmed
    ..The deletion analysis along with sequence modification of the hairpin structure indicates that the second hairpin plays a role in efficient long-distance systemic movement of TYMV...
  69. Shin H, Tzanetakis I, Dreher T, Cho T. The 5'-UTR of Turnip yellow mosaic virus does not include a critical encapsidation signal. Virology. 2009;387:427-35 pubmed publisher
  70. Jakubiec A, Yang S, Chua N. Arabidopsis DRB4 protein in antiviral defense against Turnip yellow mosaic virus infection. Plant J. 2012;69:14-25 pubmed publisher
    ..Our findings suggest that the control of viral protein as well as RNA levels might be important for mounting an efficient antiviral response...
  71. Hammond R, Ramirez P. Molecular characterization of the genome of Maize rayado fino virus, the type member of the genus Marafivirus. Virology. 2001;282:338-47 pubmed publisher
    ..Unlike OBDV, MRFV RNA does not appear to contain a poly(A) tail, and it encodes a putative second overlapping open reading frame...
  72. de Smit M, Gultyaev A, Hilge M, Bink H, Barends S, Kraal B, et al. Structural variation and functional importance of a D-loop-T-loop interaction in valine-accepting tRNA-like structures of plant viral RNAs. Nucleic Acids Res. 2002;30:4232-40 pubmed
    ..structures (TLSs) are found at the 3' ends of the genomic RNAs of most plant viruses belonging to the genera Tymovirus, Furovirus, Pomovirus and Pecluvirus, and of one Tobamovirus species...
  73. Kasschau K, Xie Z, Allen E, Llave C, Chapman E, Krizan K, et al. P1/HC-Pro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA unction. Dev Cell. 2003;4:205-17 pubmed
    ..The basis for TuMV- and other virus-induced disease in plants may be explained, at least partly, by interference with miRNA-controlled developmental pathways that share components with the antiviral RNA-silencing pathway...
  74. Matsuda D, Bauer L, Tinnesand K, Dreher T. Expression of the two nested overlapping reading frames of turnip yellow mosaic virus RNA is enhanced by a 5' cap and by 5' and 3' viral sequences. J Virol. 2004;78:9325-35 pubmed
    ..Barends et al., Cell 112:123-129, 2003)...
  75. Matsuda D, Dreher T. Cap- and initiator tRNA-dependent initiation of TYMV polyprotein synthesis by ribosomes: evaluation of the Trojan horse model for TYMV RNA translation. RNA. 2007;13:129-37 pubmed
    ..These results favor the initiation coupling model...
  76. Lorber B, Adrian M, Witz J, Erhardt M, Harris J. Formation of two-dimensional crystals of icosahedral RNA viruses. Micron. 2008;39:431-46 pubmed
    ..The formation of 2D crystals and the enhancing effect of polyethylene glycol on the self-organisation of virions at the air/water interface are discussed...
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