entomopoxvirinae

Summary

Summary: A subfamily of POXVIRIDAE comprising poxviruses infecting insects including members of COLEOPTERA; DIPTERA; LEPIDOPTERA; and ORTHOPTERA.

Top Publications

  1. Chakraborty M, Narayanan K, Sivaprakash M. In vivo enhancement of nucleopolyhedrovirus of oriental Armyworm, Mythimna separata using spindles from Helicoverpa armigera entomopoxvirus. Indian J Exp Biol. 2004;42:121-3 pubmed
    ..The enhancement index (log10) of the virus was 2.76 or reduction of more than 500 times in LC50. The ability and the mechanism of the spindles from H. armigera entomopoxvirus to enhance the infectivity of MsNPV has been discussed...
  2. Mitsuhashi W, Miyamoto K. Disintegration of the peritrophic membrane of silkworm larvae due to spindles of an entomopoxvirus. J Invertebr Pathol. 2003;82:34-40 pubmed
  3. Bawden A, Glassberg K, Diggans J, Shaw R, Farmerie W, Moyer R. Complete genomic sequence of the Amsacta moorei entomopoxvirus: analysis and comparison with other poxviruses. Virology. 2000;274:120-39 pubmed
    ..The complete genomic sequence of AmEPV paves the way for an understanding and comparison of the molecular properties and pathogenesis between the entomopoxviruses of insects and the more intensively studied vertebrate poxviruses...
  4. Osborne R, Symonds T, Sriskantha A, Lai Fook J, Fernon C, Dall D. An entomopoxvirus homologue of the vaccinia virus D13L-encoded 'rifampicin resistance' protein. J Gen Virol. 1996;77 ( Pt 5):839-46 pubmed
    ..We suggest that possession of a gene encoding a member of this polypeptide family might represent a defining molecular characteristic of the Poxviridae...
  5. Lawrence P. Purification and partial characterization of an entomopoxvirus (DLEPV) from a parasitic wasp of tephritid fruit flies. J Insect Sci. 2002;2:10 pubmed
    ..Unlike other EPVs, DlEPV does not express the spheroidin protein. Since it also replicates in both the wasp and fly, members of two different insect Orders, DlEPV may represent a new EPV Group, or a subgroup of the Group C viruses...
  6. Lawrence P. Morphogenesis and cytopathic effects of the Diachasmimorpha longicaudata entomopoxvirus in host haemocytes. J Insect Physiol. 2005;51:221-33 pubmed
    ..These data indicate that DlEPV disrupts the normal function of host haemocytes, thereby insuring the successful development of D. longicaudata offspring and as such should be regarded as a symbiont of the wasp...
  7. Winter J, Hall R, Moyer R. The effect of inhibitors on the growth of the entomopoxvirus from Amsacta moorei in Lymantria dispar (gypsy moth) cells. Virology. 1995;211:462-73 pubmed
    ..Instead, in the presence of IBT, AmEPV enveloped, immature virions form devoid of a well-differentiated core, which unlike mature virions fail to insert into occlusion bodies...
  8. Mwaengo D, Lawrence P. A putative DNA helicase and novel oligoribonuclease in the Diachasmimorpha longicaudata entomopoxvirus (DlEPV). Arch Virol. 2003;148:1431-44 pubmed
    ..These results support earlier reports that DlEPV is a member of the sub-family Entomopoxvirinae, most likely in Group C, and is the first symbiotic EPV described to date from a parasitic wasp.
  9. Hashimoto Y, Lawrence P. Comparative analysis of selected genes from Diachasmimorpha longicaudata entomopoxvirus and other poxviruses. J Insect Physiol. 2005;51:207-20 pubmed

More Information

Publications63

  1. Afonso C, Tulman E, Lu Z, Oma E, Kutish G, Rock D. The genome of Melanoplus sanguinipes entomopoxvirus. J Virol. 1999;73:533-52 pubmed
    The family Poxviridae contains two subfamilies: the Entomopoxvirinae (poxviruses of insects) and the Chordopoxvirinae (poxviruses of vertebrates)...
  2. Takemoto Y, Mitsuhashi W, Murakami R, Konishi H, Miyamoto K. The N-terminal region of an entomopoxvirus fusolin is essential for the enhancement of peroral infection, whereas the C-terminal region is eliminated in digestive juice. J Virol. 2008;82:12406-15 pubmed publisher
    ..These results suggest that the enhancement of infectivity involves more than the tolerance to digestive juice and chitin-binding ability. ..
  3. Hukuhara T, Hayakawa T, Wijonarko A. A bacterially produced virus enhancing factor from an entomopoxvirus enhances nucleopolyhedrovirus infection in armyworm larvae. J Invertebr Pathol. 2001;78:25-30 pubmed
    ..Removal of the GST moiety with glutathione-Sepharose 4B resulted in a highly purified EF preparation, which enhanced PsunMNPV infection in armyworm larvae and PsunMNPV fusion with an armyworm cell line, SIE-MSH-805-F...
  4. Crnov R, Dall D. Virions of Heliothis armigera entomopoxvirus contain a homologue of the vaccinia VP8 major core protein. Virus Genes. 1999;19:23-31 pubmed
    ..evidence for the assumption that morphological similarities observed between virions of the sub-families Entomopoxvirinae and Chordopoxvirinae by microscopy reflect corresponding similarities at a molecular level...
  5. Yu J, Sun X. Reannotation of protein-coding genes based on an improved graphical representation of DNA sequence. J Comput Chem. 2010;31:2126-35 pubmed publisher
    ..The present method could also be extended to other genomes with or without adaptation of training sets with high accuracy...
  6. Arif B, Pavlik L. Insect cell culture: virus replication and applications in biotechnology. J Invertebr Pathol. 2013;112 Suppl:S138-41 pubmed publisher
    ..Some entomopoxviruses have been replicated in a few cell lines and this paper reports the replication of an entomopoxvirus from the spruce budworm in a homologous cell line...
  7. Alaoui Ismaili M, Richardson C. Identification and characterization of a filament-associated protein encoded by Amsacta moorei entomopoxvirus. J Virol. 1996;70:2697-705 pubmed
    ..The fibrils containing this polypeptide are closely associated with occlusion bodies and may play a role in their morphogenesis and maturation...
  8. Hernández Crespo P, Veyrunes J, Cousserans F, Bergoin M. The spheroidin of an entomopoxvirus isolated from the grasshopper Anacridium aegyptium (AaEPV) shares low homology with spheroidins from lepidopteran or coleopteran EPVs. Virus Res. 2000;67:203-13 pubmed
    Based on virion morphology, the current virus taxonomy groups entomopoxviruses (EPVs) (Poxvirus: Entomopoxvirinae) from coleopteran and dipteran hosts in separated genera, wilts it keeps viruses infecting either lepidopteran or ..
  9. Lai Fook J, Dall D. Spindle bodies of Heliothis armigera entomopoxvirus develop in structures associated with host cell endoplasmic reticulum. J Invertebr Pathol. 2000;75:183-92 pubmed
    ..The ER-specific chaperone BiP has been observed to be associated with developing SBs at all stages of this process, and it is postulated that its sequestration within these bodies may have consequences for host cell metabolism...
  10. Radek R, Fabel P. A new entomopoxvirus from a cockroach: light and electron microscopy. J Invertebr Pathol. 2000;75:19-27 pubmed
    ..Taxonomically, the described virus exhibits features of both EPV genus A and EPV genus B. Provisory it is named Blattella germanica EPV (BgEPV). A possible use of the cockroach EPV as a biological control agent is discussed...
  11. Lu J, Tong J, Feng H, Huang J, Afonso C, Rock D, et al. Unique ligation properties of eukaryotic NAD+-dependent DNA ligase from Melanoplus sanguinipes entomopoxvirus. Biochim Biophys Acta. 2004;1701:37-48 pubmed
    ..The complementary specificities of these two enzymes suggest alternative primer design for genomic profiling approaches that use allele-specific detection directly from RNA transcripts...
  12. Wijonarko A, Hukuhara T. Detection of a virus enhancing factor in the spheroid, spindle, and virion of an entomopoxvirus. J Invertebr Pathol. 1998;72:82-6 pubmed
    ..An antiserum against an enhancing factor detected the homologous antigen in spheroids, spindles, and tissue-derived EPV virions but not in spheroid-derived virions...
  13. Li X, Barrett J, Yuen L, Arif B. Cloning, sequencing and transcriptional analysis of the Choristoneura fumiferana entomopoxvirus spheroidin gene. Virus Res. 1997;47:143-54 pubmed
    ..Primer extension results, anchor PCR and sequencing confirmed that there was a poly (A)17 tract at the 5' end of the spheroidin gene transcript, a structure typical of late gene transcripts of poxviruses...
  14. Li Y, Hall R, Moyer R. Transient, nonlethal expression of genes in vertebrate cells by recombinant entomopoxviruses. J Virol. 1997;71:9557-62 pubmed
    ..Therefore, AmEPV may prove to be a highly efficient, nontoxic method of gene delivery into vertebrate cells for transient gene expression...
  15. Nakai M, Shiotsuki T, Kunimi Y. An entomopoxvirus and a granulovirus use different mechanisms to prevent pupation of Adoxophyes honmai. Virus Res. 2004;101:185-91 pubmed
    ..honmai larvae. Thus, while AdhoEPV and AdhoGV both prevent pupation of A. honmai larvae, the mechanisms by which they do so appear to be different...
  16. Mitsuhashi W, Miyamoto K, Wada S. The complete genome sequence of the Alphaentomopoxvirus Anomala cuprea entomopoxvirus, including its terminal hairpin loop sequences, suggests a potentially unique mode of apoptosis inhibition and mode of DNA replication. Virology. 2014;452-453:95-116 pubmed publisher
    ..Furthermore, an ORF in the genome contains a serpin domain that was previously found in vertebrate poxviruses for apoptosis inhibition but not in insect viruses...
  17. Muratoglu H, Nalcacioglu R, Arif B, Demirbag Z. Genome-wide analysis of differential mRNA expression of Amsacta moorei entomopoxvirus, mediated by the gene encoding a viral protein kinase (AMV197). Virus Res. 2016;215:25-36 pubmed publisher
    ..Further investigations are, however, needed to narrow down the role of AMV197 throughout the infection process. ..
  18. Li Q, Liston P, Schokman N, Ho J, Moyer R. Amsacta moorei Entomopoxvirus inhibitor of apoptosis suppresses cell death by binding Grim and Hid. J Virol. 2005;79:3684-91 pubmed
    ..have been identified in the genomes of two independent families of insect viruses, the Baculoviridae and the Entomopoxvirinae. In this report, we examined the functional attributes of the Amsacta moorei entomopoxvirus-encoded IAP ..
  19. Nalcacioglu R, Dizman Y, Vlak J, Demirbag Z, van Oers M. Amsacta moorei entomopoxvirus encodes a functional DNA photolyase (AMV025). J Invertebr Pathol. 2010;105:363-5 pubmed publisher
    ..Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase...
  20. Lawrence P, Dillard B. A homolog of the vaccinia virus D13L rifampicin resistance gene is in the entomopoxvirus of the parasitic wasp, Diachasmimorpha longicaudata. J Insect Sci. 2008;8:8 pubmed publisher
    ..DlEPV remains an unassigned member of the Entomopoxvirinae (http://www.ncbi.nlm.nih.gov/ICTVdb/Ictv/index...
  21. Mitsuhashi W. Further evidence that spindles of an entomopoxvirus enhance its infectivity in a host insect. J Invertebr Pathol. 2002;79:59-61 pubmed
  22. Benarroch D, Shuman S. Characterization of mimivirus NAD+-dependent DNA ligase. Virology. 2006;353:133-43 pubmed
    ..We speculate that the dissemination of NAD+-dependent ligase from bacterium to eukaryotic virus might have occurred within an amoebal host...
  23. Mitsuhashi W, Kawakita H, Murakami R, Takemoto Y, Saiki T, Miyamoto K, et al. Spindles of an entomopoxvirus facilitate its infection of the host insect by disrupting the peritrophic membrane. J Virol. 2007;81:4235-43 pubmed
  24. Hukuhara T, Wijonarko A. Enhanced fusion of a nucleopolyhedrovirus with cultured cells by a virus enhancing factor from an entomopoxvirus. J Invertebr Pathol. 2001;77:62-7 pubmed
    ..The plasma membrane of cultured cells had specific affinity to the EF, as revealed by indirect immunofluorescence microscopy...
  25. Okuno S, Nakai M, Hiraoka T, Kunimi Y. Isolation of a protein lethal to the endoparasitoid Cotesia kariyai from entomopoxvirus-infected larvae of Mythimna separata. Insect Biochem Mol Biol. 2002;32:559-66 pubmed
    ..Western blot analysis also indicated that the proteins of virions and occlusion bodies of MyseEPV are not serologically related to PLCK...
  26. Mitsuhashi W, Sato M, Hirai Y. Involvement of spindles of an entomopoxvirus (EPV) in infectivity of the EPVs to their host insect. Arch Virol. 2000;145:1465-71 pubmed
    ..cuprea larvae. A significant increase in infectivity was induced by the addition of spindles to the spheroids, strongly suggesting that the spindles play an important role in the infection of the host insect...
  27. Krogh B, Cheng C, Burgin A, Shuman S. Melanoplus sanguinipes entomopoxvirus DNA topoisomerase: site-specific DNA transesterification and effects of 5'-bridging phosphorothiolates. Virology. 1999;264:441-51 pubmed
    ..5'-bridging phosphorothiolates at the scissile phosphate and other positions within the CCCTT element had no significant effect on k(cl)...
  28. Mitsuhashi W, Saito H, Sato M. Complete nucleotide sequence of the fusolin gene of an entomopoxvirus in the cupreous chafer, Anomala cuprea Hope (Coleoptera: Scarabaeidae). Insect Biochem Mol Biol. 1997;27:869-76 pubmed
    ..Furthermore, the last 330 bp region of the ORF revealed a low homology with that of MmEPV...
  29. Mitsuhashi W, Saito H, Sato M, Nakashima N, Noda H. Complete nucleotide sequence of spheroidin gene of Anomala cuprea entomopoxvirus. Virus Res. 1998;55:61-9 pubmed
    ..The number of cysteine residues and N-glycosylation sites also depended on the difference in genera (A or B). Thus, the spheroidin gene of EPVs was well conserved within the same genus...
  30. Benarroch D, Claverie J, Raoult D, Shuman S. Characterization of mimivirus DNA topoisomerase IB suggests horizontal gene transfer between eukaryal viruses and bacteria. J Virol. 2006;80:314-21 pubmed
  31. Hall R, Li Y, Feller J, Moyer R. The Amsacta moorei entomopoxvirus spheroidin gene is improperly transcribed in vertebrate poxviruses. Virology. 1996;224:427-36 pubmed
    ..Our results indicate that insect and vertebrate poxvirus promoters may not be universally interchangeable and imply that there are regulatory features of gene expression unique to the infected insect cell environment...
  32. Takatsuka J, Okuno S, Ishii T, Nakai M, Kunimi Y. Fitness-related traits of entomopoxviruses isolated from Adoxophyes honmai (Lepidoptera: Tortricidae) at three localities in Japan. J Invertebr Pathol. 2010;105:121-31 pubmed publisher
    ..The implications for selecting a candidate isolate to control tortricid pests are discussed...
  33. Hayakawa T, Xu J, Hukuhara T. Cloning and sequencing of the gene for an enhancing factor from Pseudaletia separata entomopoxvirus. Gene. 1996;177:269-70 pubmed
    ..The deduced amino acid sequence exhibited a significant similarity to major spindle proteins, fusolins, whose function is unknown, from Heliothis armigera, Choristoneura biennis and Melolontha melolontha entomopoxviruses...
  34. Perera S, Wong P, Krell P, Arif B. Expression of heterologous genes in the Amsacta moorei entomopoxvirus. J Virol Methods. 2010;165:1-8 pubmed publisher
    ..The highest level of reporter gene expression was observed in the recombinant containing 21 bp from the sph coding region. These results indicate that sph locus of AMEV can be used successfully to express exogenous genes...
  35. Perera S, Krell P, Demirbag Z, Nalcacioglu R, Arif B. Induction of apoptosis by the Amsacta moorei entomopoxvirus. J Gen Virol. 2013;94:1876-87 pubmed publisher
    ..A significantly reduced rate of infection was observed in the presence of general caspase inhibitors Q-VD-OPH and Z-VAD-FMK, indicating caspases may be involved in productive virus infection. ..
  36. Guo F, Yu X. Re-prediction of protein-coding genes in the genome of Amsacta moorei entomopoxvirus. J Virol Methods. 2007;146:389-92 pubmed
    ..Six of the seven falsely missed genes are less than 300bp. The present method could be extended to other poxvirus genomes with or without adaptation of training sets...
  37. Thézé J, Takatsuka J, Li Z, Gallais J, Doucet D, Arif B, et al. New insights into the evolution of Entomopoxvirinae from the complete genome sequences of four entomopoxviruses infecting Adoxophyes honmai, Choristoneura biennis, Choristoneura rosaceana, and Mythimna separata. J Virol. 2013;87:7992-8003 pubmed publisher
    Poxviruses are nucleocytoplasmic large DNA viruses encompassing two subfamilies, the Chordopoxvirinae and the Entomopoxvirinae, infecting vertebrates and insects, respectively...
  38. Li X, Wallis J, Barrett J, Krell P, Arif B. Characterization of the nucleoside triphosphate phosphohydrolase I gene from the Choristoneura fumiferana entomopoxvirus. Virus Res. 1998;56:93-105 pubmed
    ..2% of nucleotide and 97.2% of amino acid identities are observed, respectively. A single nucleotide difference in CfEPV nph I was sufficient to distinguish it from CbEPV by PCR amplification and digestion with a restriction enzyme...
  39. Hukuhara T, Hayakawa T, Wijonarko A. Increased baculovirus susceptibility of armyworm larvae feeding on transgenic rice plants expressing an entomopoxvirus gene. Nat Biotechnol. 1999;17:1122-4 pubmed
    ..Thus, introduction of the EF gene into plants can be used as a strategy to increase the effectiveness of baculoviruses in insect pest management...
  40. Li Q, Liston P, Moyer R. Functional analysis of the inhibitor of apoptosis (iap) gene carried by the entomopoxvirus of Amsacta moorei. J Virol. 2005;79:2335-45 pubmed
    ..Our results indicate that while the AMViap gene was active in controlling apoptosis through the intrinsic pathway, the virus likely encodes additional proteins that also regulate apoptosis...
  41. Palli S, Ladd T, Tomkins W, Shu S, Ramaswamy S, Tanaka Y, et al. Choristoneura fumiferana entomopoxvirus prevents metamorphosis and modulates juvenile hormone and ecdysteroid titers. Insect Biochem Mol Biol. 2000;30:869-76 pubmed
    ..Thus, our data show that EPV prevents metamorphosis by modulating ecdysteroid and JH levels...
  42. Becker M, Greenleaf W, Ostrov D, Moyer R. Amsacta moorei entomopoxvirus expresses an active superoxide dismutase. J Virol. 2004;78:10265-75 pubmed
    ..Therefore, AMVSOD is the first example of an active poxvirus SOD...
  43. Olszewski J, Dall D. Assessment of foreign protein production by recombinant Heliothis (Helicoverpa) armigera entomopoxviruses in Spodoptera frugiperda cells. J Gen Virol. 2002;83:451-61 pubmed
    ..The potential applications for further development of HaEPV expression systems are discussed...
  44. Narayanan K. Entomopoxvirus of cotton bollworm, Helicoverpa armigera (Hbn.). Indian J Exp Biol. 2002;40:842-5 pubmed
    ..Further, safety of HaEPV was shown against beneficial insect like mulberry silkworm, Bombyx mori and an useful insect general predator, Chrysoperla carnea...
  45. Gauthier L, Cousserans F, Veyrunes J, Bergoin M. The Melolontha melolontha entomopoxvirus (MmEPV) fusolin is related to the fusolins of lepidopteran EPVs and to the 37K baculovirus glycoprotein. Virology. 1995;208:427-36 pubmed
    ..This indicated that the vaccinia transcription machinery is able to transcribe the fusolin gene. The fusolin was also expressed in insect cells via a recombinant baculovirus...
  46. Palmer C, Miller D, Marlow S, Wilson L, Lawrie A, King L. Genetic modification of an entomopoxvirus: deletion of the spheroidin gene does not affect virus replication in vitro. J Gen Virol. 1995;76 ( Pt 1):15-23 pubmed
  47. Salvador R, Ferrelli M, Berretta M, Mitsuhashi W, Biedma M, Romanowski V, et al. Analysis of EpapGV gp37 gene reveals a close relationship between granulovirus and entomopoxvirus. Virus Genes. 2012;45:610-3 pubmed publisher
  48. Sanz P, Veyrunes J, Cousserans F, Bergoin M. Cloning and sequencing of the spherulin gene, the occlusion body major polypeptide of the Melolontha melolontha entomopoxvirus (MmEPV). Virology. 1994;202:449-57 pubmed
    ..These are characteristic features of vertebrate poxvirus late mRNAs suggesting similar modalities of gene expression for vertebrate and insect poxvirus genomes...
  49. Petersen B, Hall R, Moyer R, Shuman S. Characterization of a DNA topoisomerase encoded by Amsacta moore entomopoxvirus. Virology. 1997;230:197-206 pubmed
    ..The kinetic and equilibrium parameters of the DNA cleavage reaction of AmEPV topoisomerase (Kobs = 0.08 sec-1; Kcl = 0.22) are similar to those of the vaccinia virus enzyme...
  50. Sriskantha A, Osborne R, Dall D. Mapping of the Heliothis armigera entomopoxvirus (HaEPV) genome, and analysis of genes encoding the HaEPV spheroidin and nucleoside triphosphate phosphohydrolase I proteins. J Gen Virol. 1997;78 ( Pt 12):3115-23 pubmed
    ..It is proposed that genes of EPVs can be assigned to five actual or predicted homology-based groups, a categorization which is useful for directing and interpreting investigations of EPV gene functions and relationships...
  51. Becker M, Todd T, Moyer R. An Amsacta moorei entomopoxvirus ortholog of the poly(A) polymerase small subunit exhibits methyltransferase activity and is non-essential for virus growth. Virology. 2008;375:624-36 pubmed publisher
    ..No physical association was found between any of the putative AMEV PAP subunits. We therefore propose that mRNA polyadenylation does not require interactions between these three proteins...
  52. Means J, Penabaz T, Clem R. Identification and functional characterization of AMVp33, a novel homolog of the baculovirus caspase inhibitor p35 found in Amsacta moorei entomopoxvirus. Virology. 2007;358:436-47 pubmed
    ..Thus, AMVp33 encodes a caspase inhibitor similar to baculovirus P35 with a preference for effector caspases. This is the first report of a p35 homolog from any viral or cellular genome outside of the baculovirus family...
  53. Liu S, Li H, Sivakumar S, Bonning B. Virus-derived genes for insect-resistant transgenic plants. Adv Virus Res. 2006;68:427-57 pubmed
    ..Plant expression of polydnavirus-derived genes is described by Gill et al. (this volume, pp. 393-426)...
  54. Furuta Y, Mitsuhashi W, Kobayashi J, Hayasaka S, Imanishi S, Chinzei Y, et al. Peroral infectivity of non-occluded viruses of Bombyx mori nucleopolyhedrovirus and polyhedrin-negative recombinant baculoviruses to silkworm larvae is drastically enhanced when administered with Anomala cuprea entomopoxvirus spindles. J Gen Virol. 2001;82:307-12 pubmed
    ..In contrast, spheroids (proteinaceous structures containing AcEPV virions) did not enhance the peroral infectivity of either NOVs or the recombinant BmNPV in silkworm larvae...