duck hepatitis b virus

Summary

Summary: A DNA virus that closely resembles human hepatitis B virus. It has been recovered from naturally infected ducks.

Top Publications

  1. Cova L, Zoulim F. Duck hepatitis B virus model in the study of hepatitis B virus. Methods Mol Med. 2004;96:261-8 pubmed
  2. Nassal M. Hepatitis B virus replication: novel roles for virus-host interactions. Intervirology. 1999;42:100-16 pubmed
    ..Identifying these host factors and characterizing their interactions with the viral components will certainly reveal novel targets for specific antiviral strategies. ..
  3. Beck J, Vogel M, Nassal M. dNTP versus NTP discrimination by phenylalanine 451 in duck hepatitis B virus P protein indicates a common structure of the dNTP-binding pocket with other reverse transcriptases. Nucleic Acids Res. 2002;30:1679-87 pubmed
    ..This priming reaction can be reconstituted with in vitro-translated duck hepatitis B virus (DHBV) P protein. No direct structural data are available for any P protein...
  4. Addison W, Walters K, Wong W, Wilson J, Madej D, Jewell L, et al. Half-life of the duck hepatitis B virus covalently closed circular DNA pool in vivo following inhibition of viral replication. J Virol. 2002;76:6356-63 pubmed
    ..We inhibited the replication of duck hepatitis B virus in congenitally infected ducks with a combination of lamivudine and a dideoxyguanosine prodrug...
  5. Sen N, Cao F, Tavis J. Translation of duck hepatitis B virus reverse transcriptase by ribosomal shunting. J Virol. 2004;78:11751-7 pubmed
    The duck hepatitis B virus (DHBV) polymerase (P) is translated by de novo initiation from a downstream open reading frame (ORF) that partially overlaps the core (C) ORF on the bicistronic pregenomic RNA (pgRNA)...
  6. Tang H, McLachlan A. Avian and Mammalian hepadnaviruses have distinct transcription factor requirements for viral replication. J Virol. 2002;76:7468-72 pubmed
    ..In contrast, HNF3 and HNF4 support duck hepatitis B virus (DHBV) replication in nonhepatoma cells, whereas the RXR alpha-PPAR alpha heterodimer inhibits HNF4-..
  7. Habig J, Loeb D. Sequence identity of the direct repeats, DR1 and DR2, contributes to the discrimination between primer translocation and in situ priming during replication of the duck hepatitis B virus. J Mol Biol. 2006;364:32-43 pubmed
    ..sequence identity between DR1 and DR2 is necessary, but not sufficient for primer translocation in the duck hepatitis B virus. The discrimination between in situ priming and primer translocation depends upon suppression of in situ ..
  8. Le Mire M, Miller D, Foster W, Burrell C, Jilbert A. Covalently closed circular DNA is the predominant form of duck hepatitis B virus DNA that persists following transient infection. J Virol. 2005;79:12242-52 pubmed
    ..In the current study, ducks inoculated with duck hepatitis B virus (DHBV) were monitored for residual DHBV DNA following recovery from transient infection until 9 months ..
  9. Sauerbrei A, Schacke M, Schultz U, Egerer R, Merkle I, Glebe D, et al. Alternative methods for validation of cell culture infection with duck hepatitis B virus. J Virol Methods. 2005;129:178-85 pubmed
    ..Infection of cell cultures was most efficient 4 days after plating. The best identification of infected cultures was possible 6 days after infection with immunofluorescence using an antiserum against DHBV surface antigen...

More Information

Publications65

  1. Meier P, Scougall C, Will H, Burrell C, Jilbert A. A duck hepatitis B virus strain with a knockout mutation in the putative X ORF shows similar infectivity and in vivo growth characteristics to wild-type virus. Virology. 2003;317:291-8 pubmed
    Hepadnaviruses including human hepatitis B virus (HBV) and duck hepatitis B virus (DHBV) express X proteins, HBx and DHBx, respectively. Both HBx and DHBx are transcriptional activators and modulate cellular signaling in in vitro assays...
  2. Beck J, Nassal M. Efficient Hsp90-independent in vitro activation by Hsc70 and Hsp40 of duck hepatitis B virus reverse transcriptase, an assumed Hsp90 client protein. J Biol Chem. 2003;278:36128-38 pubmed
    ..Using an in vitro reconstitution system consisting of recombinant duck hepatitis B virus RT, purified chaperones, and the authentic RNA template Depsilon, we demonstrate here that this RT can be ..
  3. Tavis J, Ganem D. Evidence for activation of the hepatitis B virus polymerase by binding of its RNA template. J Virol. 1996;70:5741-50 pubmed
    ..b>Duck hepatitis B virus polymerase expressed in Saccharomyces cerevisiae can synthesize DNA from epsilon-containing RNAs and can ..
  4. Wang X, Qian X, Guo H, Hu J. Heat shock protein 90-independent activation of truncated hepadnavirus reverse transcriptase. J Virol. 2003;77:4471-80 pubmed
    ..Using the RT from duck hepatitis B virus as a model, we previously demonstrated that RT-epsilon interaction and protein priming require the ..
  5. Loeb D, Mack A, Tian R. A secondary structure that contains the 5' and 3' splice sites suppresses splicing of duck hepatitis B virus pregenomic RNA. J Virol. 2002;76:10195-202 pubmed
    ..pgRNA is a terminally redundant transcript whose synthesis does not involve RNA splicing. For duck hepatitis B virus (DHBV), a spliced RNA is derived from pgRNA by removal of a single intron...
  6. Urban S, Gripon P. Inhibition of duck hepatitis B virus infection by a myristoylated pre-S peptide of the large viral surface protein. J Virol. 2002;76:1986-90 pubmed
    We have used the duck hepatitis B virus (DHBV) model to study the interference with infection by a myristoylated peptide representing an N-terminal pre-S subdomain of the large viral envelope protein...
  7. Spangenberg H, Lee H, Li J, Tan F, Skidgel R, Wands J, et al. A short sequence within domain C of duck carboxypeptidase D is critical for duck hepatitis B virus binding and determines host specificity. J Virol. 2001;75:10630-42 pubmed
    ..At least in the case of duck hepatitis B virus (DHBV), host specificity seems controlled partly at the receptor level...
  8. Rösler C, Kock J, Kann M, Malim M, Blum H, Baumert T, et al. APOBEC-mediated interference with hepadnavirus production. Hepatology. 2005;42:301-9 pubmed
    ..In contrast to HIV and other retroviruses, however, APOBEC3G/3F-mediated editing of nucleic acids does not seem to represent an effective innate defense mechanism for hepadnaviruses...
  9. Loeb D, Tian R. Mutations that increase in situ priming also decrease circularization for duck hepatitis B virus. J Virol. 2001;75:6492-7 pubmed
    ..This type of synthesis is called in situ priming. Although in situ priming is normally low, in some duck hepatitis B virus mutants this type of priming is elevated...
  10. Grgacic E, Schaller H. A metastable form of the large envelope protein of duck hepatitis B virus: low-pH release results in a transition to a hydrophobic, potentially fusogenic conformation. J Virol. 2000;74:5116-22 pubmed
    We have examined the structure and fusion potential of the duck hepatitis B virus (DHBV) envelope proteins by treating subviral particles with deforming agents known to release envelope proteins of viruses from a metastable to a fusion-..
  11. Ampt K, van der Werf R, Nelissen F, Tessari M, Wijmenga S. The unstable part of the apical stem of duck hepatitis B virus epsilon shows enhanced base pair opening but not pico- to nanosecond dynamics and is essential for reverse transcriptase binding. Biochemistry. 2009;48:10499-508 pubmed publisher
    ..The unstable secondary structure of the apical stem-loop makes duck epsilon-RT binding unusual in light of recent classifications of RNA target interactions that assume stable secondary structures...
  12. Cao F, Badtke M, Metzger L, Yao E, Adeyemo B, Gong Y, et al. Identification of an essential molecular contact point on the duck hepatitis B virus reverse transcriptase. J Virol. 2005;79:10164-70 pubmed
    ..the exposure of the epitopes of six monoclonal antibodies (MAbs) to the terminal protein domain of the duck hepatitis B virus P protein in a partially denaturing buffer (RIPA) and a physiological buffer (IPP150)...
  13. Nassal M, Leifer I, Wingert I, Dallmeier K, Prinz S, Vorreiter J. A structural model for duck hepatitis B virus core protein derived by extensive mutagenesis. J Virol. 2007;81:13218-29 pubmed
    b>Duck hepatitis B virus (DHBV) shares many fundamental features with human HBV...
  14. Robaczewska M, Narayan R, Seigneres B, Schorr O, Thermet A, Podhajska A, et al. Sequence-specific inhibition of duck hepatitis B virus reverse transcription by peptide nucleic acids (PNA). J Hepatol. 2005;42:180-7 pubmed
    ..Our aim was to study the ability of PNAs targeting the duck HBV (DHBV) encapsidation signal epsilon to inhibit reverse transcription (RT) and to compare their efficacy with phosphorothioate oligodeoxynucleotides (S-ODNs)...
  15. Mabit H, Knaust A, Breiner K, Schaller H. Nuclear localization of the duck hepatitis B virus capsid protein: detection and functional implications of distinct subnuclear bodies in a compartment associated with RNA synthesis and maturation. J Virol. 2003;77:2157-64 pubmed
    We have reinvestigated the subcellular distribution of the duck hepatitis B virus (DHBV) core protein in infected duck hepatocytes and in transfected cells...
  16. Thermet A, Robaczewska M, Rollier C, Hantz O, Trepo C, Deleage G, et al. Identification of antigenic regions of duck hepatitis B virus core protein with antibodies elicited by DNA immunization and chronic infection. J Virol. 2004;78:1945-53 pubmed
    The induction of humoral response in ducks by DNA-based immunization against duck hepatitis B virus (DHBV) core protein (DHBc) was investigated...
  17. Tohidi Esfahani R, Vickery K, Cossart Y. The early host innate immune response to duck hepatitis B virus infection. J Gen Virol. 2010;91:509-20 pubmed publisher
    ..This work compared the early phase of duck hepatitis B virus infection in 1-day-old (D1) and 28-day-old (D28) ducks to determine whether differences in viral or host ..
  18. Srivastav N, Shakya N, Mak M, Agrawal B, Tyrrell D, Kumar R. Antiviral activity of various 1-(2'-deoxy-?-D-lyxofuranosyl), 1-(2'-fluoro-?-D-xylofuranosyl), 1-(3'-fluoro-?-D-arabinofuranosyl), and 2'-fluoro-2',3'-didehydro-2',3'-dideoxyribose pyrimidine nucleoside analogues against duck hepatitis B virus (DHBV). J Med Chem. 2010;53:7156-66 pubmed publisher
    ..1 ?M. In contrast, 50% inhibition could not be achieved by lamivudine at 44 ?M concentration in the drug-resistant strain. The compounds investigated did not show cytotoxicity to host cells up to the highest concentrations tested...
  19. Narayan R, Buronfosse T, Schultz U, Chevallier Gueyron P, Guerret S, Chevallier M, et al. Rise in gamma interferon expression during resolution of duck hepatitis B virus infection. J Gen Virol. 2006;87:3225-32 pubmed
  20. Miller D, Boyle D, Feng F, Reaiche G, Kotlarski I, Colonno R, et al. Antiviral therapy with entecavir combined with post-exposure "prime-boost" vaccination eliminates duck hepatitis B virus-infected hepatocytes and prevents the development of persistent infection. Virology. 2008;373:329-41 pubmed publisher
    Short-term antiviral therapy with the nucleoside analogue entecavir (ETV), given at an early stage of duck hepatitis B virus (DHBV) infection, restricts virus spread and leads to clearance of DHBV-infected hepatocytes in approximately 50% ..
  21. Hu J, Flores D, Toft D, Wang X, Nguyen D. Requirement of heat shock protein 90 for human hepatitis B virus reverse transcriptase function. J Virol. 2004;78:13122-31 pubmed
    ..RT-epsilon interaction in the duck hepatitis B virus (DHBV) was recently shown to require the molecular chaperone complex, the heat shock protein 90 (Hsp90)...
  22. Zimmerman K, Fischer K, Joyce M, Tyrrell D. Zinc finger proteins designed to specifically target duck hepatitis B virus covalently closed circular DNA inhibit viral transcription in tissue culture. J Virol. 2008;82:8013-21 pubmed publisher
    b>Duck hepatitis B virus (DHBV) is a model virus for human hepatitis B virus (HBV), which infects approximately 360 million individuals worldwide...
  23. Zhang Y, Summers J. Rapid production of neutralizing antibody leads to transient hepadnavirus infection. J Virol. 2004;78:1195-201 pubmed
    ..The basis of these age-related outcomes is not known. Infection of ducks with duck hepatitis B virus (DHBV) displays a similar dependence of outcome on the age of the host at the time of infection...
  24. Jilbert A, Botten J, Miller D, Bertram E, Hall P, Kotlarski J, et al. Characterization of age- and dose-related outcomes of duck hepatitis B virus infection. Virology. 1998;244:273-82 pubmed
    Experimental inoculation of naive ducks with duck hepatitis B virus (DHBV) can lead to one of three outcomes, namely, persistent viremia, transient infection with or without viremia, or no evidence of infection...
  25. Kock J, Kann M, Putz G, Blum H, Von Weizsacker F. Central role of a serine phosphorylation site within duck hepatitis B virus core protein for capsid trafficking and genome release. J Biol Chem. 2003;278:28123-9 pubmed
    ..Here, we used the duck hepatitis B virus (DHBV) infection model to analyze the effects of capsid phosphorylation and hydrogen bond formation...
  26. Li J, Tong S, Lee H, Perdigoto A, Spangenberg H, Wands J. Glycine decarboxylase mediates a postbinding step in duck hepatitis B virus infection. J Virol. 2004;78:1873-81 pubmed
    ..Two binding partners for duck hepatitis B virus (DHBV) pre-S envelope protein have been identified...
  27. Perlman D, Hu J. Duck hepatitis B virus virion secretion requires a double-stranded DNA genome. J Virol. 2003;77:2287-94 pubmed
    ..Using a synchronized replication system for the duck hepatitis B virus, together with a stringent two-step assay for virion secretion, we demonstrate that this selective genome ..
  28. Hu J, Toft D, Seeger C. Hepadnavirus assembly and reverse transcription require a multi-component chaperone complex which is incorporated into nucleocapsids. EMBO J. 1997;16:59-68 pubmed
    ..We have shown recently that the formation of this RNP complex in an avian hepadnavirus, the duck hepatitis B virus, depends on cellular factors that include the heat shock protein 90 (Hsp90)...
  29. Sauerbrei A, Schacke M, Gluck B, Egerer R, Wutzler P. Validation of biocides against duck hepatitis B virus as a surrogate virus for human hepatitis B virus. J Hosp Infect. 2006;64:358-65 pubmed
    The use of a surrogate virus, namely duck hepatitis B virus (DHBV), has been recommended for testing the virucidal activity of chemical biocides against hepatitis B virus...
  30. Yao E, Tavis J. Kinetics of synthesis and turnover of the duck hepatitis B virus reverse transcriptase. J Biol Chem. 2003;278:1201-5 pubmed
    ..Previously, we found that duck hepatitis B virus polymerase unexpectedly accumulates in the cytoplasm (Yao, E., Gong, Y., Chen, N., and Tavis, J. E...
  31. Hu J, Anselmo D. In vitro reconstitution of a functional duck hepatitis B virus reverse transcriptase: posttranslational activation by Hsp90. J Virol. 2000;74:11447-55 pubmed
    ..understand the mechanism of RT activation by Hsp90, we have now mapped the minimal RT sequences of the duck hepatitis B virus that are required for chaperone binding, RNP formation, and protein priming...
  32. Beck J, Nassal M. Hepatitis B virus replication. World J Gastroenterol. 2007;13:48-64 pubmed
    ..The ultimate, though most challenging goal, will be to visualize the hepadnaviral reverse transcriptase in the act of synthesizing DNA, which will also have strong implications for drug development...
  33. Qiao M, Scougall C, Duszynski A, Burrell C. Kinetics of early molecular events in duck hepatitis B virus replication in primary duck hepatocytes. J Gen Virol. 1999;80 ( Pt 8):2127-35 pubmed
    This paper describes the use of one-step growth conditions to study the kinetics of duck hepatitis B virus (DHBV) replication in primary duck hepatocytes. Synchronized infection was achieved using partially purified DHBV virions at an m.o...
  34. Zhang Y, Zhang B, Theele D, Litwin S, Toll E, Summers J. Single-cell analysis of covalently closed circular DNA copy numbers in a hepadnavirus-infected liver. Proc Natl Acad Sci U S A. 2003;100:12372-7 pubmed
    ..Using a PCR-based assay, we examined the number of cccDNA molecules of the duck hepatitis B virus in single nuclei isolated from the liver of a chronically infected duck over the course of 131 days of ..
  35. Beck J, Nassal M. Reconstitution of a functional duck hepatitis B virus replication initiation complex from separate reverse transcriptase domains expressed in Escherichia coli. J Virol. 2001;75:7410-9 pubmed
    ..Currently, the only system for generating such complexes in the test tube is in vitro translation of duck hepatitis B virus (DHBV) P protein in rabbit reticulocyte lysate (RRL), which also provides the necessary factors...
  36. Liu W, Zhai J, Liu J, Xie Y. Identification of natural recombination in duck hepatitis B virus. Virus Res. 2010;149:245-51 pubmed publisher
    ..The identification of inter-genotypic recombination among circulating DHBV isolates suggests the usefulness of DHBV as a model for studying the mechanism of HBV recombination...
  37. Funk A, Mhamdi M, Hohenberg H, Heeren J, Reimer R, Lambert C, et al. Duck hepatitis B virus requires cholesterol for endosomal escape during virus entry. J Virol. 2008;82:10532-42 pubmed publisher
    ..We show that the duck hepatitis B virus (DHBV) attaches predominantly to detergent-soluble domains on the plasma membrane...
  38. Choi J, Kim E, Park Y, Han Y. Expression of the active human and duck hepatitis B virus polymerases in heterologous system of Pichia methanolica. Antiviral Res. 2002;55:279-90 pubmed
    ..HBV (1-680 amino acids) and Duck Hepatitis B virus (DHBV, 1-780 amino acids) polymerase were expressed and showed DNA dependent DNA polymerase (DDDP)...
  39. Walters K, Joyce M, Addison W, Fischer K, Tyrrell D. Superinfection exclusion in duck hepatitis B virus infection is mediated by the large surface antigen. J Virol. 2004;78:7925-37 pubmed
    ..The Pekin duck hepatitis B virus (DHBV) model was used to investigate superinfection exclusion in hepadnavirus infections...
  40. Glebe D, Urban S. Viral and cellular determinants involved in hepadnaviral entry. World J Gastroenterol. 2007;13:22-38 pubmed
    ..g. HBV) and avihepadnaviruses (e.g. duck hepatitis B virus [DHBV])...
  41. Breiner K, Schaller H, Knolle P. Endothelial cell-mediated uptake of a hepatitis B virus: a new concept of liver targeting of hepatotropic microorganisms. Hepatology. 2001;34:803-8 pubmed
    ..Using a model hepatitis B virus, the duck hepatitis B virus (DHBV), we have obtained data indicating that scavenging liver sinusoidal endothelial cells (LSEC), rather ..
  42. Beck J, Nassal M. Sequence- and structure-specific determinants in the interaction between the RNA encapsidation signal and reverse transcriptase of avian hepatitis B viruses. J Virol. 1997;71:4971-80 pubmed
  43. Vorreiter J, Leifer I, Rösler C, Jackevica L, Pumpens P, Nassal M. Monoclonal antibodies providing topological information on the duck hepatitis B virus core protein and avihepadnaviral nucleocapsid structure. J Virol. 2007;81:13230-4 pubmed
    The icosahedral capsid of duck hepatitis B virus (DHBV) is formed by a single core protein species (DHBc). DHBc is much larger than HBc from human HBV, and no high-resolution structure is available. In an accompanying study (M. Nassal, I...
  44. Schultz U, Grgacic E, Nassal M. Duck hepatitis B virus: an invaluable model system for HBV infection. Adv Virus Res. 2004;63:1-70 pubmed
  45. Hu K, Beck J, Nassal M. SELEX-derived aptamers of the duck hepatitis B virus RNA encapsidation signal distinguish critical and non-critical residues for productive initiation of reverse transcription. Nucleic Acids Res. 2004;32:4377-89 pubmed
  46. Girard F, Ottink O, Ampt K, Tessari M, Wijmenga S. Thermodynamics and NMR studies on Duck, Heron and Human HBV encapsidation signals. Nucleic Acids Res. 2007;35:2800-11 pubmed
    ..All avian ALs are expected to adhere to this because of their conserved sequence. Duck PL is stable and structured and, in view of sequence similarities, the same is expected for heron - and human PL...
  47. Tavis J, Massey B, Gong Y. The duck hepatitis B virus polymerase is activated by its RNA packaging signal, epsilon. J Virol. 1998;72:5789-96 pubmed
    ..Three lines of evidence employing duck hepatitis B virus supporting this hypothesis are presented here...
  48. Anderson D, Grgacic E, Luscombe C, Gu X, Dixon R. Quantification of infectious duck hepatitis B virus by radioimmunofocus assay. J Med Virol. 1997;52:354-61 pubmed
    A simple method is described for the precise quantification of infectious duck hepatitis B virus (DHBV) in cell culture, using a radioimmunofocus assay (RIFA)...
  49. Saade F, Buronfosse T, Pradat P, Abdul F, Cova L. Enhancement of neutralizing humoral response of DNA vaccine against duck hepatitis B virus envelope protein by co-delivery of cytokine genes. Vaccine. 2008;26:5159-64 pubmed publisher
    We explored in the duck hepatitis B virus (DHBV) model the impact of duck interferon gamma (Du-IFNgamma) or interleukin 2 (Du-IL2) co-delivery on humoral neutralizing response induced by DNA-based vaccine encoding DHBV preS/S large ..
  50. Beck J, Nassal M. In vitro reconstitution of epsilon-dependent duck hepatitis B virus replication initiation. Methods Mol Med. 2004;95:315-25 pubmed
  51. Gong Y, Yao E, Tavis J. Evidence that the RNAseH activity of the duck hepatitis B virus is unable to act on exogenous substrates. BMC Microbiol. 2001;1:12 pubmed
    ..The mechanism of this template commitment is unknown. Here we provide evidence that the RNAseH activity of duck hepatitis B virus reverse transcriptase may also be unable to act on exogenous substrates.
  52. Tong Y, Tong S, Zhao X, Wang J, Jun J, Park J, et al. Initiation of duck hepatitis B virus infection requires cleavage by a furin-like protease. J Virol. 2010;84:4569-78 pubmed publisher
    ..HBV infection has both host and tissue specificities. For the related duck hepatitis B virus (DHBV), duck carboxypeptidase D (DCPD) has been proposed as the species-specific docking receptor, while ..
  53. Guo J, Pryce M, Wang X, Barrasa M, Hu J, Seeger C. Conditional replication of duck hepatitis B virus in hepatoma cells. J Virol. 2003;77:1885-93 pubmed
    ..in the hepadnavirus system, we have developed cell lines permitting the conditional replication of duck hepatitis B virus (DHBV)...
  54. Miller D, Kotlarski I, Jilbert A. DNA vaccines expressing the duck hepatitis B virus surface proteins lead to reduced numbers of infected hepatocytes and protect ducks against the development of chronic infection in a virus dose-dependent manner. Virology. 2006;351:159-69 pubmed
    We tested the efficacy of DNA vaccines expressing the duck hepatitis B virus (DHBV) pre-surface (pre-S/S) and surface (S) proteins in modifying the outcome of infection in 14-day-old ducks...
  55. Mabit H, Breiner K, Knaust A, Zachmann Brand B, Schaller H. Signals for bidirectional nucleocytoplasmic transport in the duck hepatitis B virus capsid protein. J Virol. 2001;75:1968-77 pubmed
    ..In this study, we analyze the signals determining capsid compartmentalization in the duck hepatitis B virus (DHBV) animal model, as this system also allows us to study hepadnavirus infection of cultured primary ..
  56. Jilbert A, Miller D, Scougall C, Turnbull H, Burrell C. Kinetics of duck hepatitis B virus infection following low dose virus inoculation: one virus DNA genome is infectious in neonatal ducks. Virology. 1996;226:338-45 pubmed
    Using pooled serum from congenitally duck hepatitis B virus (DHBV)-infected ducks as inoculum, we examined the effect of virus dose on the incubation period of infection and on the patterns of spread of virus infection in the liver...