baculoviridae

Summary

Summary: Family of INSECT VIRUSES containing two subfamilies: Eubaculovirinae (occluded baculoviruses) and Nudibaculovirinae (nonoccluded baculoviruses). The Eubaculovirinae, which contain polyhedron-shaped inclusion bodies, have two genera: NUCLEOPOLYHEDROVIRUS and GRANULOVIRUS. Baculovirus vectors are used for expression of foreign genes in insects.

Top Publications

  1. Lopez Macias C, Ferat Osorio E, Tenorio Calvo A, Isibasi A, Talavera J, Arteaga Ruiz O, et al. Safety and immunogenicity of a virus-like particle pandemic influenza A (H1N1) 2009 vaccine in a blinded, randomized, placebo-controlled trial of adults in Mexico. Vaccine. 2011;29:7826-34 pubmed publisher
    ..Based on these data, additional clinical trials are in development to evaluate influenza vaccine candidate antigens manufactured using Spodoptera frugiperda (Sf9)/baculovirus-based VLP technology...
  2. Ikeda M, Yamada H, Hamajima R, Kobayashi M. Baculovirus genes modulating intracellular innate antiviral immunity of lepidopteran insect cells. Virology. 2013;435:1-13 pubmed publisher
  3. Temeyer K, Chen A. Acetylcholinesterase of Stomoxys calcitrans (L.) (Diptera: Muscidae): cDNA sequence, baculovirus expression, and biochemical properties. Vet Parasitol. 2012;184:92-5 pubmed publisher
  4. Zhu B, Ye J, Lu P, Jiang R, Yang X, Fu Z, et al. Induction of antigen-specific immune responses in mice by recombinant baculovirus expressing premembrane and envelope proteins of West Nile virus. Virol J. 2012;9:132 pubmed publisher
    ..Recently baculovirus has emerged as a novel and attractive gene delivery vehicle for mammalian cells...
  5. Garavaglia M, Miele S, Iserte J, Belaich M, Ghiringhelli P. The ac53, ac78, ac101, and ac103 genes are newly discovered core genes in the family Baculoviridae. J Virol. 2012;86:12069-79 pubmed publisher
    The family Baculoviridae is a large group of insect viruses containing circular double-stranded DNA genomes of 80 to 180 kbp, which have broad biotechnological applications...
  6. Luo W, Shih Y, Hung C, Lo K, Chiang C, Lo W, et al. Development of the hybrid Sleeping Beauty: baculovirus vector for sustained gene expression and cancer therapy. Gene Ther. 2012;19:844-51 pubmed publisher
    ..These data altogether implicated the potential of the hybrid SB-baculovirus vector for prolonged hEA expression and for the treatment of multiple types of angiogenesis-dependent tumors...
  7. Sparks M, Gundersen Rindal D. The Lymantria dispar IPLB-Ld652Y cell line transcriptome comprises diverse virus-associated transcripts. Viruses. 2011;3:2339-50 pubmed publisher
    ..These sequence resources will provide a sound basis for developing testable experimental hypotheses by insect virologists, and suggest a number of avenues for potential research...
  8. Bieniossek C, Imasaki T, Takagi Y, Berger I. MultiBac: expanding the research toolbox for multiprotein complexes. Trends Biochem Sci. 2012;37:49-57 pubmed publisher
    ..Among other applications, MultiBac has been used to produce many important proteins and their complexes for their structural characterization, revealing fundamental cellular mechanisms...
  9. Wang Y, Choi J, Roh J, Liu Q, Tao X, Park J, et al. Genomic sequence analysis of granulovirus isolated from the tobacco cutworm, Spodoptera litura. PLoS ONE. 2011;6:e28163 pubmed publisher
    ..It is a major pest of many crops. To better understand Spodoptera litura granulovirus (SpliGV), the nucleotide sequence of the SpliGV DNA genome was determined and analyzed...

More Information

Publications80

  1. Hou D, Zhang L, Deng F, Fang W, Wang R, Liu X, et al. Comparative proteomics reveal fundamental structural and functional differences between the two progeny phenotypes of a baculovirus. J Virol. 2013;87:829-39 pubmed publisher
    ..This comparative proteomics study provides new insight into how BV and ODV are formed and why they function differently...
  2. Carinhas N, Robitaille A, Moes S, Carrondo M, Jenoe P, Oliveira R, et al. Quantitative proteomics of Spodoptera frugiperda cells during growth and baculovirus infection. PLoS ONE. 2011;6:e26444 pubmed publisher
    ..These findings have implications for virological research and improvement of baculovirus-based bioprocesses...
  3. Katsuma S, Koyano Y, Kang W, Kokusho R, Kamita S, Shimada T. The baculovirus uses a captured host phosphatase to induce enhanced locomotory activity in host caterpillars. PLoS Pathog. 2012;8:e1002644 pubmed publisher
    ..Collectively, we show that the modern baculovirus uses the host-derived phosphatase to establish adequate infection for ELA as a virion-associated structural protein rather than as an enzyme...
  4. Chen G, Hwang S, Su H, Kuo C, Luo W, Lo K, et al. Defective antiviral responses of induced pluripotent stem cells to baculoviral vector transduction. J Virol. 2012;86:8041-9 pubmed publisher
    ..These data together confirmed that iPSCs responded poorly to BV due to the impaired sensing and signaling system, thereby justifying the transduction of iPSCs with the baculoviral vector...
  5. Temeyer K, Brake D, Schlechte K. Acetylcholinesterase of Haematobia irritans (Diptera: Muscidae): baculovirus expression, biochemical properties, and organophosphate insensitivity of the G262A mutant. J Med Entomol. 2012;49:589-94 pubmed
  6. Molinari P, Crespo M, Gravisaco M, Taboga O, Moron G. Baculovirus capsid display potentiates OVA cytotoxic and innate immune responses. PLoS ONE. 2011;6:e24108 pubmed publisher
    ..All these findings, together with the absence of pre-existing immunity to BV in humans and the lack of viral gene expression in mammalian cells, make BV a candidate for vaccination...
  7. Hitchman R, Locanto E, Possee R, King L. Optimizing the baculovirus expression vector system. Methods. 2011;55:52-7 pubmed publisher
    ..The bacmid has also been genetically optimized for improved protein expression and stability. We describe the application of this system for high level production of recombinant proteins...
  8. Syed M, Kwang J. Oral vaccination of baculovirus-expressed VP28 displays enhanced protection against White Spot Syndrome Virus in Penaeus monodon. PLoS ONE. 2011;6:e26428 pubmed publisher
    ..Our findings indicate that oral vaccination of shrimp with Bac-VP28 is an attractive preventative measure against WSSV infection that can be used in the field...
  9. Lin C, Chang Y, Kao C, Lu C, Sung L, Yen T, et al. Augmented healing of critical-size calvarial defects by baculovirus-engineered MSCs that persistently express growth factors. Biomaterials. 2012;33:3682-92 pubmed publisher
    ..These data implicated the potential of the hybrid BV vector to engineer BMSCs for sustained BMP2/VEGF expression and the repair of critical-size calvarial defects...
  10. Airenne K, Hu Y, Kost T, Smith R, Kotin R, Ono C, et al. Baculovirus: an insect-derived vector for diverse gene transfer applications. Mol Ther. 2013;21:739-49 pubmed publisher
    ..Stem cell transduction, preclinical animal studies, tissue engineering, vaccination, cancer gene therapy, viral vector production, and drug discovery are covered...
  11. Nguyen Q, Chan L, Nielsen L, Reid S. Genome scale analysis of differential mRNA expression of Helicoverpa zea insect cells infected with a H. armigera baculovirus. Virology. 2013;444:158-70 pubmed publisher
    ..Differential expression was linked to changes in the number of intracellular and extracellular viral genomes and occlusion bodies. The first comprehensive elucidation of HearNPV-H. zea expression kinetics was obtained...
  12. Huynh H, Tran T, Chan L, Nielsen L, Reid S. Decline in baculovirus-expressed recombinant protein production with increasing cell density is strongly correlated to impairment of virus replication and mRNA expression. Appl Microbiol Biotechnol. 2013;97:5245-57 pubmed publisher
    ..Such limitations may be largely nutritional, but a more complex mechanism may be implicated...
  13. Nguyen Q, Palfreyman R, Chan L, Reid S, Nielsen L. Transcriptome sequencing of and microarray development for a Helicoverpa zea cell line to investigate in vitro insect cell-baculovirus interactions. PLoS ONE. 2012;7:e36324 pubmed publisher
  14. Zoth S, Gómez E, Carballeda J, Carrillo E, Berinstein A. Expression of a secreted version of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: its evaluation as a diagnostic reagent. J Vet Diagn Invest. 2011;23:519-23 pubmed publisher
    ..The present results show that the cloning strategy developed yielded the HN protein free in the cell culture supernatant and that the recombinant protein retained its reactivity with anti-NDV HN antibodies in chicken sera...
  15. Fuller E, Elderd B, Dwyer G. Pathogen persistence in the environment and insect-baculovirus interactions: disease-density thresholds, epidemic burnout, and insect outbreaks. Am Nat. 2012;179:E70-96 pubmed publisher
    ..Our work suggests that estimates of persistence times can lead to a deeper understanding of environmentally transmitted pathogens and illustrates the usefulness of experiments that are closely tied to mathematical models...
  16. Rychlowska M, Gromadzka B, Bienkowska Szewczyk K, Szewczyk B. Application of baculovirus-insect cell expression system for human therapy. Curr Pharm Biotechnol. 2011;12:1840-9 pubmed
  17. Mehrabadi M, Hussain M, Asgari S. MicroRNAome of Spodoptera frugiperda cells (Sf9) and its alteration following baculovirus infection. J Gen Virol. 2013;94:1385-97 pubmed publisher
    ..Seed shifting and arm switching happened during evolution of miRNAs in different insects and caused miRNA diversification, which led to changes in the target repository of miRNAs...
  18. Paul A, Binsalamah Z, Khan A, Abbasia S, Elias C, Shum Tim D, et al. A nanobiohybrid complex of recombinant baculovirus and Tat/DNA nanoparticles for delivery of Ang-1 transgene in myocardial infarction therapy. Biomaterials. 2011;32:8304-18 pubmed publisher
    ..77% vs 37.46 ± 5.2%, p < 0.01), NP(Ang1) and the control group (32.26 ± 2.49% and 31.58 ± 2.26%). Collectively, this data demonstrates hybrid Bac-NP as a new and improved gene delivery system for therapeutic applications...
  19. Paul A, Shao W, Abbasi S, Shum Tim D, Prakash S. PAMAM dendrimer-baculovirus nanocomplex for microencapsulated adipose stem cell-gene therapy: in vitro and in vivo functional assessment. Mol Pharm. 2012;9:2479-88 pubmed publisher
    ..6 ± 6.1%) compared to that in MC+ASC-MGFP (38.8 ± 5.3%) and MC groups (31.5 ± 3.3%). Collectively, these data demonstrate the feasibility of this system for improved stem cell therapy applications...
  20. Sokolenko S, George S, Wagner A, Tuladhar A, Andrich J, Aucoin M. Co-expression vs. co-infection using baculovirus expression vectors in insect cell culture: Benefits and drawbacks. Biotechnol Adv. 2012;30:766-81 pubmed publisher
    ..The review concludes with an examination of the gaps that still remain in co-expression/co-infection knowledge and practice...
  21. Cox M. Recombinant protein vaccines produced in insect cells. Vaccine. 2012;30:1759-66 pubmed publisher
    ..Dependent on health care priorities, different vaccines could be manufactured while maintaining the ability to rapidly convert to producing pandemic influenza vaccine when the need arises...
  22. Breitenbach J, Shelby K, Popham H. Baculovirus induced transcripts in hemocytes from the larvae of Heliothis virescens. Viruses. 2011;3:2047-64 pubmed publisher
    ..Using this method, we completed the first and most comprehensive gene expression survey of both baculoviral infection and host immune defense in lepidopteran larvae...
  23. Au S, Pante N. Nuclear transport of baculovirus: revealing the nuclear pore complex passage. J Struct Biol. 2012;177:90-8 pubmed publisher
    ..Our data suggests the NPC central channel undergoes large-scale rearrangements to allow translocation of the intact 250-nm long baculovirus capsid. We discuss our results in the light of the hypothetical models of NPC function...
  24. Metz S, Feenstra F, Villoing S, van Hulten M, van Lent J, Koumans J, et al. Low temperature-dependent salmonid alphavirus glycoprotein processing and recombinant virus-like particle formation. PLoS ONE. 2011;6:e25816 pubmed publisher
    ..So, temperature shift-down during synthesis is a prerequisite for correct SAV glycoprotein processing and recombinant VLP production...
  25. Mena J, Kamen A. Insect cell technology is a versatile and robust vaccine manufacturing platform. Expert Rev Vaccines. 2011;10:1063-81 pubmed publisher
    ..We will probably observe increasingly more baculovirus-derived products and market licensing of safe and efficacious vaccines...
  26. Popham H, Sun R, Shelby K, Robertson J. Iron levels change in larval Heliothis virescens tissues following baculovirus infection. Biol Trace Elem Res. 2012;148:356-62 pubmed publisher
    ..In both studies, hemolymph Fe levels were higher in HzSNPV infected larvae. This first study of tissue Fe levels during viral infection of an insect clearly demonstrates that Fe homeostasis is substantially disrupted...
  27. Premanand B, Kiener T, Meng T, Tan Y, Jia Q, Chow V, et al. Induction of protective immune responses against EV71 in mice by baculovirus encoding a novel expression cassette for capsid protein VP1. Antiviral Res. 2012;95:311-5 pubmed publisher
    ..This study revealed that baculovirus displaying VP1 with a HA transmembrane domain efficiently induced cross-neutralizing antibody responses in mice...
  28. Paul A, Nayan M, Khan A, Shum Tim D, Prakash S. Angiopoietin-1-expressing adipose stem cells genetically modified with baculovirus nanocomplex: investigation in rat heart with acute infarction. Int J Nanomedicine. 2012;7:663-82 pubmed publisher
    ..The study identified Bac-NP complex as an advanced gene delivery vehicle for stem cells and demonstrated its potential to treat ischemic heart disease with high therapeutic index for combined stem cell-gene therapy strategy...
  29. van Houte S, Ros V, Mastenbroek T, Vendrig N, Hoover K, Spitzen J, et al. Protein tyrosine phosphatase-induced hyperactivity is a conserved strategy of a subset of baculoviruses to manipulate lepidopteran host behavior. PLoS ONE. 2012;7:e46933 pubmed publisher
    ..Overall, these data provide a firm base for a deeper understanding of the mechanisms behind baculovirus-induced insect behavior...
  30. Yang Y, Lo S, Yang J, Yang J, Goh S, Wu C, et al. Polyethylenimine coating to produce serum-resistant baculoviral vectors for in vivo gene delivery. Biomaterials. 2009;30:5767-74 pubmed publisher
    ..The method may also serve as a flexible platform technology for improved use of the vectors, for example introducing a targeting ligand and minimizing immune responses. ..
  31. Millán A, Gomez Sebastian S, Núñez M, Veramendi J, Escribano J. Human papillomavirus-like particles vaccine efficiently produced in a non-fermentative system based on insect larva. Protein Expr Purif. 2010;74:1-8 pubmed publisher
  32. Gomez Casado E, Gomez Sebastian S, Nunez M, Lasa Covarrubias R, Martínez Pulgarín S, Escribano J. Insect larvae biofactories as a platform for influenza vaccine production. Protein Expr Purif. 2011;79:35-43 pubmed publisher
  33. Carinhas N, Bernal V, Monteiro F, Carrondo M, Oliveira R, Alves P. Improving baculovirus production at high cell density through manipulation of energy metabolism. Metab Eng. 2010;12:39-52 pubmed publisher
    ..These results represent a cost-efficient and scalable strategy for high cell density production of recombinant baculovirus vectors. ..
  34. Carinhas N, Bernal V, Yokomizo A, Carrondo M, Oliveira R, Alves P. Baculovirus production for gene therapy: the role of cell density, multiplicity of infection and medium exchange. Appl Microbiol Biotechnol. 2009;81:1041-9 pubmed publisher
    ..Virus titers as high as 2.6 x 10(10) IP x mL(-1) were obtained in cultures infected at 3.5 x 10(6) cells x mL(-1), while the amplification factor was roughly 19 times higher than the highest value obtained without medium exchange. ..
  35. Vicente T, Roldão A, Peixoto C, Carrondo M, Alves P. Large-scale production and purification of VLP-based vaccines. J Invertebr Pathol. 2011;107 Suppl:S42-8 pubmed publisher
    ..This paper reviews the current choices and trends of large-scale production and purification of VLP-based vaccines generated through the baculovirus expression vector system using insect cells. ..
  36. Kim Y, Choi J, Jiang H, Arote R, Jere D, Cho M, et al. Hybrid of baculovirus and galactosylated PEI for efficient gene carrier. Virology. 2009;387:89-97 pubmed publisher
    ..These results suggest that the GP/B hybrid system can be used in gene therapy to enhance transduction efficiency and hepatocyte specificity. ..
  37. Huang H, Xiao S, Qin J, Jiang Y, Yang S, Li T, et al. Construction and immunogenicity of a recombinant pseudotype baculovirus expressing the glycoprotein of rabies virus in mice. Arch Virol. 2011;156:753-8 pubmed publisher
    ..Our data clearly indicate that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop a new generation of vaccine against RABV infection. ..
  38. Chen C, Luo W, Lo W, Lin K, Sung L, Shih Y, et al. Development of hybrid baculovirus vectors for artificial MicroRNA delivery and prolonged gene suppression. Biotechnol Bioeng. 2011;108:2958-67 pubmed publisher
  39. Hebert C, Valdes J, Bentley W. Investigating apoptosis: characterization and analysis of Trichoplusia ni-caspase-1 through overexpression and RNAi mediated silencing. Insect Biochem Mol Biol. 2009;39:113-24 pubmed publisher
    ..Finally, our results indicate differences between RNAi and p35 as effector molecules for modulating caspase activity and apoptosis during cell growth and baculovirus infection. ..
  40. Chazarra S, Aznar Cervantes S, Sánchez del Campo L, Cabezas Herrera J, Xiaofeng W, Cenis J, et al. Purification and kinetic properties of human recombinant dihydrofolate reductase produced in Bombyx mori chrysalides. Appl Biochem Biotechnol. 2010;162:1834-46 pubmed publisher
    ..Expression of proteins in chrysalides (instead of larvae) could offer important advantages from both economic and biosecurity aspects. ..
  41. He F, Ho Y, Yu L, Kwang J. WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine. BMC Microbiol. 2008;8:238 pubmed publisher
    ..The worldwide outbreak of influenza A (H5N1) viruses among poultry species and humans highlighted the need to develop efficacious and safe vaccines based on efficient and scaleable production...
  42. Bézier A, Annaheim M, Herbinière J, Wetterwald C, Gyapay G, Bernard Samain S, et al. Polydnaviruses of braconid wasps derive from an ancestral nudivirus. Science. 2009;323:926-30 pubmed publisher
    ..We found that the nudiviral genes themselves are no longer packaged but are actively transcribed and produce particles used to deliver genes essential for successful parasitism in lepidopteran hosts. ..
  43. Shelby K, Popham H. Plasma phenoloxidase of the larval tobacco budworm, Heliothis virescens, is virucidal. J Insect Sci. 2006;6:1-12 pubmed publisher
    ..Our results confirm that phenoloxidase is the predominate activity in larval plasma accounting for inactivation of HzSNPV in vitro, and that phenoloxidase-dependent H(2)O(2) production may contribute to this virucidal activity. ..
  44. Ramachandra C, Shahbazi M, Kwang T, Choudhury Y, Bak X, Yang J, et al. Efficient recombinase-mediated cassette exchange at the AAVS1 locus in human embryonic stem cells using baculoviral vectors. Nucleic Acids Res. 2011;39:e107 pubmed publisher
    ..The method developed will be useful for repeated gene targeting at a defined locus of the hESC genome. ..
  45. He F, Madhan S, Kwang J. Baculovirus vector as a delivery vehicle for influenza vaccines. Expert Rev Vaccines. 2009;8:455-67 pubmed publisher
  46. Lin C, Lu C, Luo W, Chang Y, Sung L, Chiu H, et al. Baculovirus as a gene delivery vector for cartilage and bone tissue engineering. Curr Gene Ther. 2010;10:242-54 pubmed
    ..This article reviews the studies pertaining to the applications of baculovirus-mediated gene delivery in cartilage and bone tissue engineering and discusses recent progress, future applications and potential hurdles. ..
  47. Yao L, Sun J, Xu H, Kan Y, Zhang X, Yan H. A novel economic method for high throughput production of recombinant baculovirus by infecting insect cells with Bacmid-containing diminopimelate-auxotrophic Escherichia coli. J Biotechnol. 2010;145:23-9 pubmed publisher
    ..coli and the labour-intensive and complex transfection procedure, this transfection reagent free method enables a convenient and economic high throughput production of recombinant baculoviruses. ..
  48. Yoshida S, Kawasaki M, Hariguchi N, Hirota K, Matsumoto M. A baculovirus dual expression system-based malaria vaccine induces strong protection against Plasmodium berghei sporozoite challenge in mice. Infect Immun. 2009;77:1782-9 pubmed publisher
    ..These results introduce a novel concept for the baculovirus dual expression system that functions as both a subunit vaccine and a DNA vaccine and offer a promising new alternative to current human vaccine delivery platforms...
  49. Xue J, Salem T, Turney C, Cheng X. Strategy of the use of 28S rRNA as a housekeeping gene in real-time quantitative PCR analysis of gene transcription in insect cells infected by viruses. J Virol Methods. 2010;163:210-5 pubmed publisher
    ..Incorporation of the 28S reverse primer in oligo-dT-primed cDNA synthesis also does not interfere with the detection of other polymerase II transcribed genes...
  50. Lo W, Hwang S, Chuang C, Chen C, Hu Y. Development of a hybrid baculoviral vector for sustained transgene expression. Mol Ther. 2009;17:658-66 pubmed publisher
    ..These data collectively implicate the potential of this hybrid baculovirus system in gene therapy applications necessitating sustained transgene expression...
  51. Keil G, Klopfleisch C, Giesow K, Blohm U. Novel vectors for simultaneous high-level dual protein expression in vertebrate and insect cells by recombinant baculoviruses. J Virol Methods. 2009;160:132-7 pubmed publisher
    ..However, for expression in vertebrate cells the murine ie1 enhancer/promoter upstream the baculoviral p10 promoter was most efficient...
  52. Hebert C, Valdes J, Bentley W. In vitro and in vivo RNA interference mediated suppression of Tn-caspase-1 for improved recombinant protein production in High Five cell culture with the baculovirus expression vector system. Biotechnol Bioeng. 2009;104:390-9 pubmed publisher
    ..This cell line, designated dsTncasp-2, demonstrates superior viability under low nutrient culture conditions and resulted in as much as two times the protein yield when compared to standard High Five cells...
  53. Fang R, Feng H, Nie H, Wang L, Tu P, Song Q, et al. Construction and immunogenicity of pseudotype baculovirus expressing Toxoplasma gondii SAG1 protein in BALB/c mice model. Vaccine. 2010;28:1803-7 pubmed publisher
    ..These results indicated that pseudotype baculovirus-mediated gene delivery can be utilized as an alternative strategy to develop new generation of vaccines against T. gondii infection...
  54. Chung C, Chen C, Lin S, Chung Y, Chiu H, Chi W, et al. Enterovirus 71 virus-like particle vaccine: improved production conditions for enhanced yield. Vaccine. 2010;28:6951-7 pubmed publisher
    ..Altogether, Bac-P1-C3CD and the bioprocess render mass production more economical, obviate the need for cell lysis and hold promise for future industrial vaccine production...
  55. Marek M, van Oers M, Devaraj F, Vlak J, Merten O. Engineering of baculovirus vectors for the manufacture of virion-free biopharmaceuticals. Biotechnol Bioeng. 2011;108:1056-67 pubmed publisher
    ..This novel expression method obviates the need to purify the virions away from the biopharmaceuticals...
  56. Hakim M, Mandelbaum A, Fass D. Structure of a baculovirus sulfhydryl oxidase, a highly divergent member of the erv flavoenzyme family. J Virol. 2011;85:9406-13 pubmed publisher
    ..The diversification of the Erv sulfhydryl oxidase enzymes in large double-stranded DNA viruses exemplifies the extreme degree to which these viruses can push the boundaries of protein family folds...
  57. Lesch H, Laitinen A, Peixoto C, Vicente T, Makkonen K, Laitinen L, et al. Production and purification of lentiviral vectors generated in 293T suspension cells with baculoviral vectors. Gene Ther. 2011;18:531-8 pubmed publisher
    ..Furthermore, a scalable and cost-effective capture purification step was developed based on a diethylaminoethyl monolithic column capable of removing most of the baculoviruses from the LV pool with 65% recovery...
  58. Grabherr R, Ernst W. Baculovirus for eukaryotic protein display. Curr Gene Ther. 2010;10:195-200 pubmed
  59. Madhan S, Prabakaran M, Kwang J. Baculovirus as vaccine vectors. Curr Gene Ther. 2010;10:201-13 pubmed
    ..Gaining additional insight into the baculovirus biology and its interaction with non-native hosts will certainly promote this human-friendly virus as a potential vector for clinical applications...
  60. Lin C, Chang Y, Lin K, Yen T, Tai C, Chen C, et al. The healing of critical-sized femoral segmental bone defects in rabbits using baculovirus-engineered mesenchymal stem cells. Biomaterials. 2010;31:3222-30 pubmed publisher
  61. Paul A, Jardin B, Kulamarva A, Malhotra M, Elias C, Prakash S. Recombinant baculovirus as a highly potent vector for gene therapy of human colorectal carcinoma: molecular cloning, expression, and in vitro characterization. Mol Biotechnol. 2010;45:129-39 pubmed publisher
  62. Ji X, Sutton G, Evans G, Axford D, Owen R, Stuart D. How baculovirus polyhedra fit square pegs into round holes to robustly package viruses. EMBO J. 2010;29:505-14 pubmed publisher
    ..The use of flexible adaptors to generate a powerful system for packaging irregular particles is characteristic of the AcMNPV polyhedrin and may provide a vehicle to sequester a wide range of objects such as biological nano-particles...
  63. Jarvis D. Baculovirus-insect cell expression systems. Methods Enzymol. 2009;463:191-222 pubmed publisher
  64. Martyn J, Cardin A, Wines B, Cendron A, Li S, Mackenzie J, et al. Surface display of IgG Fc on baculovirus vectors enhances binding to antigen-presenting cells and cell lines expressing Fc receptors. Arch Virol. 2009;154:1129-38 pubmed publisher
    ..These novel baculoviral vectors, which target cells of the immune system that express FcRs, have potential applications for vaccination or gene therapy...
  65. Jin R, Lv Z, Chen Q, Quan Y, Zhang H, Li S, et al. Safety and immunogenicity of H5N1 influenza vaccine based on baculovirus surface display system of Bombyx mori. PLoS ONE. 2008;3:e3933 pubmed publisher
    ..375 mg/kg in rats and 3.2 mg/kg in cynomolgus monkeys. Our work showed the vaccine may be a candidate for a highly effective, cheap, and safe influenza vaccine for use in humans...
  66. Krammer F, Grabherr R. Alternative influenza vaccines made by insect cells. Trends Mol Med. 2010;16:313-20 pubmed publisher
    ..Three different approaches to insect cell-derived influenza vaccines have been established that could contribute to future influenza vaccine supply and development...
  67. Abe T, Matsuura Y. Host innate immune responses induced by baculovirus in mammals. Curr Gene Ther. 2010;10:226-31 pubmed
    ..This review deals with the current status of our knowledge of the induction of host innate immune responses by baculovirus and discusses the future prospects for baculovirus vectors...
  68. Noad R, Stewart M, Boyce M, Celma C, Willison K, Roy P. Multigene expression of protein complexes by iterative modification of genomic Bacmid DNA. BMC Mol Biol. 2009;10:87 pubmed publisher
    ..However current methods for selecting such recombinant genomes are too inefficient to routinely modify the virus in this way...
  69. Hitchman R, Possee R, Crombie A, Chambers A, Ho K, Siaterli E, et al. Genetic modification of a baculovirus vector for increased expression in insect cells. Cell Biol Toxicol. 2010;26:57-68 pubmed publisher
    ..These results confirm that there is still great potential for improving the BEVS, further increasing recombinant protein expression yields and stability in insect cells...
  70. Li Y, Ye J, Cao S, Xiao S, Zhao Q, Liu X, et al. Immunization with pseudotype baculovirus expressing envelope protein of Japanese encephalitis virus elicits protective immunity in mice. J Gene Med. 2009;11:57-65 pubmed publisher
    ..In the present study, we constructed a recombinant pseudotype baculovirus encoding the JEV envelope (E) protein and demonstrated that it could elicit high protective immunity in mice...
  71. Cho B, Jeon B, Kim J, Noh J, Kim J, Park M, et al. Expression and evaluation of Chikungunya virus E1 and E2 envelope proteins for serodiagnosis of Chikungunya virus infection. Yonsei Med J. 2008;49:828-35 pubmed publisher