bacteriophage t7

Summary

Summary: Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.

Top Publications

  1. Mekler V, Minakhin L, Sheppard C, Wigneshweraraj S, Severinov K. Molecular mechanism of transcription inhibition by phage T7 gp2 protein. J Mol Biol. 2011;413:1016-27 pubmed publisher
    ..The inhibition of RNAP interactions with single-stranded segments of the transcription bubble by gp2 is a novel effect, which may occur via allosteric mechanism that is set in motion by the gp2 binding to the ?' jaw. ..
  2. Hamdan S, Johnson D, Tanner N, Lee J, Qimron U, Tabor S, et al. Dynamic DNA helicase-DNA polymerase interactions assure processive replication fork movement. Mol Cell. 2007;27:539-49 pubmed
    A single copy of bacteriophage T7 DNA polymerase and DNA helicase advance the replication fork with a processivity greater than 17,000 nucleotides...
  3. Moak M, Molineux I. Peptidoglycan hydrolytic activities associated with bacteriophage virions. Mol Microbiol. 2004;51:1169-83 pubmed
    ..These studies lead to a general mechanism describing how phage genomes are transported across the bacterial cell wall...
  4. Zhu B, Lee S, Tan M, Wang E, Richardson C. Gene 5.5 protein of bacteriophage T7 in complex with Escherichia coli nucleoid protein H-NS and transfer RNA masks transfer RNA priming in T7 DNA replication. Proc Natl Acad Sci U S A. 2012;109:8050-5 pubmed publisher
    ..A deficiency in the primase of bacteriophage T7 to synthesize primers can be overcome by genetic alterations that decrease the expression of T7 gene 5...
  5. Tanner N, Loparo J, Hamdan S, Jergic S, Dixon N, van Oijen A. Real-time single-molecule observation of rolling-circle DNA replication. Nucleic Acids Res. 2009;37:e27 pubmed publisher
    ..This method allows for rapid and precise characterization of the kinetics of DNA synthesis and the effects of replication inhibitors. ..
  6. Lee J, Hite R, Hamdan S, Xie X, Richardson C, van Oijen A. DNA primase acts as a molecular brake in DNA replication. Nature. 2006;439:621-4 pubmed
    ..Here we use single-molecule techniques to study the kinetics of a multiprotein replication complex from bacteriophage T7 and to characterize the effect of primase activity on fork progression...
  7. Chan L, Kosuri S, Endy D. Refactoring bacteriophage T7. Mol Syst Biol. 2005;1:2005.0018 pubmed
    ..Here, we redesign the genome of a natural biological system, bacteriophage T7, in order to specify an engineered surrogate that, if viable, would be easier to study and extend...
  8. Qimron U, Kulczyk A, Hamdan S, Tabor S, Richardson C. Inadequate inhibition of host RNA polymerase restricts T7 bacteriophage growth on hosts overexpressing udk. Mol Microbiol. 2008;67:448-57 pubmed
    ..5 that affect its interaction with T7 RNA polymerase, also reduce the interference with T7 growth by host RNA polymerase. We propose a general model for the requirement of host RNA polymerase inhibition. ..
  9. Springman R, Badgett M, Molineux I, Bull J. Gene order constrains adaptation in bacteriophage T7. Virology. 2005;341:141-52 pubmed
    ..We adapted a bacteriophage T7 variant harboring an ectopically positioned RNA polymerase gene to determine whether it could regain the ..

More Information

Publications80

  1. Springman R, Keller T, Molineux I, Bull J. Evolution at a high imposed mutation rate: adaptation obscures the load in phage T7. Genetics. 2010;184:221-32 pubmed publisher
    ..The impact of a high mutation rate on long-term viral fitness was tested here. A large population of the DNA bacteriophage T7 was grown with a mutagen, producing a genomic rate of 4 nonlethal mutations per generation, two to three ..
  2. Guan C, Kumar S, Kucera R, Ewel A. Changing the enzymatic activity of T7 endonuclease by mutations at the beta-bridge site: alteration of substrate specificity profile and metal ion requirements by mutation distant from the catalytic domain. Biochemistry. 2004;43:4313-22 pubmed
    ..Our results suggest that the enzyme recognizes its substrates by DNA conformation exclusion and offer a simple explanation for the broad substrate specificity of phage resolvase. ..
  3. Bull J, Heineman R, Wilke C. The phenotype-fitness map in experimental evolution of phages. PLoS ONE. 2011;6:e27796 pubmed publisher
    ..In conjunction with data provided here, the new understanding appears to resolve a discrepancy between the reported fitness of phage T7 and the substantially lower value calculated from its phenotype-fitness map. ..
  4. Zhang H, Lee S, Richardson C. The roles of tryptophans in primer synthesis by the DNA primase of bacteriophage T7. J Biol Chem. 2012;287:23644-56 pubmed publisher
    ..Five tryptophan residues are dispersed in the primase of bacteriophage T7: Trp-42 in the ZBD and Trp-69, -97, -147, and -255 in the RNA polymerase domain...
  5. Donmez I, Rajagopal V, Jeong Y, Patel S. Nucleic acid unwinding by hepatitis C virus and bacteriophage t7 helicases is sensitive to base pair stability. J Biol Chem. 2007;282:21116-23 pubmed
    ..Modeling of the dependence provided the degree of the active involvement of helicase in base pair destabilization during the unwinding process and distinguished between passive and active mechanisms of unwinding. ..
  6. Johnson D, Bai L, Smith B, Patel S, Wang M. Single-molecule studies reveal dynamics of DNA unwinding by the ring-shaped T7 helicase. Cell. 2007;129:1299-309 pubmed
    ..However, an active unwinding model fully supports the data even though the helicase on its own does not unwind at its optimal rate. This work offers insights into possible ways helicase activity is enhanced by associated proteins. ..
  7. Crampton D, Mukherjee S, Richardson C. DNA-induced switch from independent to sequential dTTP hydrolysis in the bacteriophage T7 DNA helicase. Mol Cell. 2006;21:165-74 pubmed
    We show that the mechanisms of DNA-dependent and -independent dTTP hydrolysis by the gene 4 protein of bacteriophage T7 differ in the pathways by which these reactions are catalyzed...
  8. Lee S, Richardson C. Acidic residues in the nucleotide-binding site of the bacteriophage T7 DNA primase. J Biol Chem. 2005;280:26984-91 pubmed publisher
    ..Like other prokaryotic homologs, the primase domain of the gene 4 helicase-primase of bacteriophage T7 contains a zinc motif and a catalytic core...
  9. Agirrezabala X, Velazquez Muriel J, Gomez Puertas P, Scheres S, Carazo J, Carrascosa J. Quasi-atomic model of bacteriophage t7 procapsid shell: insights into the structure and evolution of a basic fold. Structure. 2007;15:461-72 pubmed
    ..are emerging as one such family, and we have studied the possible existence of the HK97-like fold in bacteriophage T7. The procapsid structure at approximately 10 A resolution was used to obtain a quasi-atomic model by fitting ..
  10. Johnson D, Takahashi M, Hamdan S, Lee S, Richardson C. Exchange of DNA polymerases at the replication fork of bacteriophage T7. Proc Natl Acad Sci U S A. 2007;104:5312-7 pubmed
  11. Lee S, Marintcheva B, Hamdan S, Richardson C. The C-terminal residues of bacteriophage T7 gene 4 helicase-primase coordinate helicase and DNA polymerase activities. J Biol Chem. 2006;281:25841-9 pubmed
    The gene 4 protein of bacteriophage T7 plays a central role in DNA replication by providing both helicase and primase activities...
  12. Tan G, Yusoff K, Seow H, Tan W. Antigenicity and immunogenicity of the immunodominant region of hepatitis B surface antigen displayed on bacteriophage T7. J Med Virol. 2005;77:475-80 pubmed
    ..The recombinant phage exhibited the antigenic and immunogenic characteristics of HBsAg, illustrating its potential as an immunological reagent and vaccine...
  13. Dimitri A, Goodenough A, Guengerich F, Broyde S, Scicchitano D. Transcription processing at 1,N2-ethenoguanine by human RNA polymerase II and bacteriophage T7 RNA polymerase. J Mol Biol. 2008;375:353-66 pubmed
    ..Our results show that 1,N(2)-epsilon G acts as a partial block to the bacteriophage T7 RNA polymerase (RNAP), which allows nucleotide incorporation in the growing RNA with the selectivity A>G&..
  14. Tong Y, Zhang Z, Liu B, Huang J, Liu H, Liu Y, et al. Autoantibodies as potential biomarkers for nasopharyngeal carcinoma. Proteomics. 2008;8:3185-93 pubmed publisher
    ..Studies of the corresponding proteins may have significances in tumor biology, novel drug development, and immunotherapy...
  15. Pandey M, Syed S, Donmez I, Patel G, Ha T, Patel S. Coordinating DNA replication by means of priming loop and differential synthesis rate. Nature. 2009;462:940-3 pubmed publisher
  16. Stano N, Patel S. T7 lysozyme represses T7 RNA polymerase transcription by destabilizing the open complex during initiation. J Biol Chem. 2004;279:16136-43 pubmed
    b>Bacteriophage T7 lysozyme binds to T7 RNA polymerase and inhibits transcription initiation and the transition from initiation to elongation...
  17. Kosuri S, Kelly J, Endy D. TABASCO: A single molecule, base-pair resolved gene expression simulator. BMC Bioinformatics. 2007;8:480 pubmed
    ..However, for practical reasons including computational tractability, available simulators have not been able to represent genome-scale models of gene expression at this level of detail...
  18. Stephanou A, Roberts G, Tock M, Pritchard E, Turkington R, Nutley M, et al. A mutational analysis of DNA mimicry by ocr, the gene 0.3 antirestriction protein of bacteriophage T7. Biochem Biophys Res Commun. 2009;378:129-32 pubmed publisher
    The ocr protein of bacteriophage T7 is a structural and electrostatic mimic of approximately 24 base pairs of double-stranded B-form DNA...
  19. Alcantara E, Kim D, Do S, Lee S. Bi-functional activities of chimeric lysozymes constructed by domain swapping between bacteriophage T7 and K11 lysozymes. J Biochem Mol Biol. 2007;40:539-46 pubmed
    The lysozymes encoded by bacteriophage T7 and K11 are both bifunctional enzymes sharing an extensive sequence homology (75%)...
  20. Cámara B, Liu M, Reynolds J, Shadrin A, Liu B, Kwok K, et al. T7 phage protein Gp2 inhibits the Escherichia coli RNA polymerase by antagonizing stable DNA strand separation near the transcription start site. Proc Natl Acad Sci U S A. 2010;107:2247-52 pubmed publisher
  21. Lee S, Richardson C. Molecular basis for recognition of nucleoside triphosphate by gene 4 helicase of bacteriophage T7. J Biol Chem. 2010;285:31462-71 pubmed publisher
    ..Although most helicases use ATP in these processes, the DNA helicase encoded by gene 4 of bacteriophage T7 uses dTTP most efficiently...
  22. Akabayov B, Akabayov S, Lee S, Tabor S, Kulczyk A, Richardson C. Conformational dynamics of bacteriophage T7 DNA polymerase and its processivity factor, Escherichia coli thioredoxin. Proc Natl Acad Sci U S A. 2010;107:15033-8 pubmed publisher
    Gene 5 of bacteriophage T7 encodes a DNA polymerase (gp5) responsible for the replication of the phage DNA. Gp5 polymerizes nucleotides with low processivity, dissociating after the incorporation of 1 to 50 nucleotides...
  23. Kiro R, Molshanski Mor S, Yosef I, Milam S, Erickson H, Qimron U. Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division. Proc Natl Acad Sci U S A. 2013;110:19549-54 pubmed publisher
    ..4 enhances the bacteriophage's competitive ability. This division inhibition is thus a fascinating example of a strategy in bacteriophages to maximize utilization of their hosts' cell resources. ..
  24. Donmez I, Patel S. Mechanisms of a ring shaped helicase. Nucleic Acids Res. 2006;34:4216-24 pubmed
    b>Bacteriophage T7 helicase (T7 gene 4 helicase-primase) is a prototypical member of the ring-shaped family of helicases, whose structure and biochemical mechanisms have been studied in detail...
  25. Krumpe L, Atkinson A, Smythers G, Kandel A, Schumacher K, McMahon J, et al. T7 lytic phage-displayed peptide libraries exhibit less sequence bias than M13 filamentous phage-displayed peptide libraries. Proteomics. 2006;6:4210-22 pubmed
    ..To demonstrate their utility, several of the T7-displayed peptide libraries were screened for streptavidin- and neutravidin-binding phage. Novel binding motifs were identified for each protein...
  26. Crampton D, Ohi M, Qimron U, Walz T, Richardson C. Oligomeric states of bacteriophage T7 gene 4 primase/helicase. J Mol Biol. 2006;360:667-77 pubmed
    Electron microscopic and crystallographic data have shown that the gene 4 primase/helicase encoded by bacteriophage T7 can form both hexamers and heptamers...
  27. Scholl D, Adhya S, Merril C. Escherichia coli K1's capsule is a barrier to bacteriophage T7. Appl Environ Microbiol. 2005;71:4872-4 pubmed
    ..This observation suggests that the capsule plays an important role as a defense against some phages that recognize structures beneath it and that the K1-specific phages evolved to counter this physical barrier...
  28. Jeong Y, Levin M, Patel S. The DNA-unwinding mechanism of the ring helicase of bacteriophage T7. Proc Natl Acad Sci U S A. 2004;101:7264-9 pubmed
    ..b>Bacteriophage T7 helicase functions as a hexameric ring to drive the replication complex by separating the DNA strands during ..
  29. Kemp P, Gupta M, Molineux I. Bacteriophage T7 DNA ejection into cells is initiated by an enzyme-like mechanism. Mol Microbiol. 2004;53:1251-65 pubmed
    In a normal infection about 850 bp of the bacteriophage T7 genome is ejected into the cell, the remainder of the genome is internalized through transcription by Escherichia coli and then T7 RNA polymerase...
  30. He Z, Richardson C. Effect of single-stranded DNA-binding proteins on the helicase and primase activities of the bacteriophage T7 gene 4 protein. J Biol Chem. 2004;279:22190-7 pubmed
    Gene 4 protein (gp4) of bacteriophage T7 provides two essential functions at the T7 replication fork, primase and helicase activities. Previous studies have shown that the single-stranded DNA-binding protein of T7, encoded by gene 2...
  31. Zhu B, Lee S, Richardson C. An in trans interaction at the interface of the helicase and primase domains of the hexameric gene 4 protein of bacteriophage T7 modulates their activities. J Biol Chem. 2009;284:23842-51 pubmed publisher
    ..The two activities reside in a single polypeptide encoded by gene 4 of bacteriophage T7. Their coexistence within the same polypeptide facilitates their coordination during DNA replication...
  32. Kemp P, Garcia L, Molineux I. Changes in bacteriophage T7 virion structure at the initiation of infection. Virology. 2005;340:307-17 pubmed
    Five proteins are ejected from the bacteriophage T7 virion at the initiation of infection...
  33. Liao J, Jeong Y, Kim D, Patel S, Oster G. Mechanochemistry of t7 DNA helicase. J Mol Biol. 2005;350:452-75 pubmed
    The bacteriophage T7 helicase is a ring-shaped hexameric motor protein that unwinds double-stranded DNA during DNA replication and recombination...
  34. Choi J, Guengerich F. Analysis of the effect of bulk at N2-alkylguanine DNA adducts on catalytic efficiency and fidelity of the processive DNA polymerases bacteriophage T7 exonuclease- and HIV-1 reverse transcriptase. J Biol Chem. 2004;279:19217-29 pubmed
    ..with the processive DNA polymerases human immunodeficiency virus, type 1, reverse transcriptase (RT), and bacteriophage T7 exonuclease(-) (T7(-))...
  35. Lee S, Richardson C. Choreography of bacteriophage T7 DNA replication. Curr Opin Chem Biol. 2011;15:580-6 pubmed publisher
    ..The lagging strand is synthesized discontinuously within a loop that forms and resolves during each cycle of Okazaki fragment synthesis. The synthesis of a primer as well as the termination of a fragment signal loop resolution...
  36. Agirrezabala X, Mart n Benito J, Valle M, Gonz lez J, Valencia A, Valpuesta J, et al. Structure of the connector of bacteriophage T7 at 8A resolution: structural homologies of a basic component of a DNA translocating machinery. J Mol Biol. 2005;347:895-902 pubmed publisher
    The three-dimensional structure of the bacteriophage T7 head-to-tail connector has been obtained at 8A resolution using cryo-electron microscopy and single-particle analysis from purified recombinant connectors...
  37. Stephanou A, Roberts G, Cooper L, Clarke D, Thomson A, Mackay C, et al. Dissection of the DNA mimicry of the bacteriophage T7 Ocr protein using chemical modification. J Mol Biol. 2009;391:565-76 pubmed publisher
    The homodimeric Ocr (overcome classical restriction) protein of bacteriophage T7 is a molecular mimic of double-stranded DNA and a highly effective competitive inhibitor of the bacterial type I restriction/modification system...
  38. Hamdan S, Richardson C. Motors, switches, and contacts in the replisome. Annu Rev Biochem. 2009;78:205-43 pubmed publisher
    ..The replisome of bacteriophage T7 contains a minimum of proteins, thus facilitating its study...
  39. Satapathy A, Crampton D, Beauchamp B, Richardson C. Promiscuous usage of nucleotides by the DNA helicase of bacteriophage T7: determinants of nucleotide specificity. J Biol Chem. 2009;284:14286-95 pubmed publisher
    The multifunctional protein encoded by gene 4 of bacteriophage T7 (gp4) provides both helicase and primase activity at the replication fork...
  40. Bull J, Molineux I, Wilke C. Slow fitness recovery in a codon-modified viral genome. Mol Biol Evol. 2012;29:2997-3004 pubmed
    ..Overall, the study supports the premise that codon-modified viruses recover fitness slowly, although the evolution is substantially more rapid than expected from the design principle...
  41. Zang H, Harris T, Guengerich F. Kinetics of nucleotide incorporation opposite polycyclic aromatic hydrocarbon-DNA adducts by processive bacteriophage T7 DNA polymerase. Chem Res Toxicol. 2005;18:389-400 pubmed
    ..The effect of these bulky PAH adducts is either to attenuate rates of conformational changes or to introduce an additional conformation problem but not to alter the inherent affinity of the polymerase for DNA or dNTPs...
  42. Zhu B, Lee S, Richardson C. Bypass of a nick by the replisome of bacteriophage T7. J Biol Chem. 2011;286:28488-97 pubmed publisher
    ..In bacteriophage T7, movement of either the DNA helicase or the DNA polymerase alone terminates upon encountering a nick in ..
  43. Qimron U, Marintcheva B, Tabor S, Richardson C. Genomewide screens for Escherichia coli genes affecting growth of T7 bacteriophage. Proc Natl Acad Sci U S A. 2006;103:19039-44 pubmed
    ..coli lipopolysaccharide receptor for T7 adsorption leads to T7 resistance. Selection of T7 phage that can recognize these altered receptors has enabled the construction of phage to which the host is 100-fold less resistant...
  44. Lin T, Chen T. A positive charge at position 33 of thioredoxin primarily affects its interaction with other proteins but not redox potential. Biochemistry. 2004;43:945-52 pubmed
    ..Together with the G33D mutation, the overall results show that a charged residue at the first X has a greater influence on the molecular interaction of the protein than the redox potential...
  45. Chang C, Kemp P, Molineux I. Gp15 and gp16 cooperate in translocating bacteriophage T7 DNA into the infected cell. Virology. 2010;398:176-86 pubmed publisher
    Loss of up to four amino acids from the C terminus of the 1318 residue bacteriophage T7 gp16 allows plaque formation at normal efficiencies...
  46. Hamdan S, Marintcheva B, Cook T, Lee S, Tabor S, Richardson C. A unique loop in T7 DNA polymerase mediates the binding of helicase-primase, DNA binding protein, and processivity factor. Proc Natl Acad Sci U S A. 2005;102:5096-101 pubmed publisher
    b>Bacteriophage T7 DNA polymerase (gene 5 protein, gp5) interacts with its processivity factor, Escherichia coli thioredoxin, via a unique loop at the tip of the thumb subdomain...
  47. Ishikawa H, Hoshino Y, Motoki Y, Kawahara T, Kitajima M, Kitami M, et al. A system for the directed evolution of the insecticidal protein from Bacillus thuringiensis. Mol Biotechnol. 2007;36:90-101 pubmed
    ..These results indicate that a method combining T7 phage display with selection using cadherin-like protein-coated magnetic beads can be used to increase the activity of easily obtained, low-activity Cry toxins from bacteria...
  48. Ghosh S, Marintcheva B, Takahashi M, Richardson C. C-terminal phenylalanine of bacteriophage T7 single-stranded DNA-binding protein is essential for strand displacement synthesis by T7 DNA polymerase at a nick in DNA. J Biol Chem. 2009;284:30339-49 pubmed publisher
    Single-stranded DNA-binding protein (gp2.5), encoded by gene 2.5 of bacteriophage T7, plays an essential role in DNA replication...
  49. Lee S, Richardson C. The linker region between the helicase and primase domains of the gene 4 protein of bacteriophage T7. Role in helicase conformation and activity. J Biol Chem. 2004;279:23384-93 pubmed
    The gene 4 protein of bacteriophage T7 provides both helicase and primase activities...
  50. Shadrin A, Sheppard C, Severinov K, Matthews S, Wigneshweraraj S. Substitutions in the Escherichia coli RNA polymerase inhibitor T7 Gp2 that allow inhibition of transcription when the primary interaction interface between Gp2 and RNA polymerase becomes compromised. Microbiology. 2012;158:2753-64 pubmed publisher
    The Escherichia coli-infecting bacteriophage T7 encodes a 7 kDa protein, called Gp2, which is a potent inhibitor of the host RNA polymerase (RNAp). Gp2 is essential for T7 phage development. The interaction site for Gp2 on the E...
  51. Stano N, Jeong Y, Donmez I, Tummalapalli P, Levin M, Patel S. DNA synthesis provides the driving force to accelerate DNA unwinding by a helicase. Nature. 2005;435:370-3 pubmed
    ..The ring-shaped helicase of bacteriophage T7 translocates along single-stranded (ss)DNA at a speed of 130 bases per second; however, T7 helicase slows ..
  52. Lee S, Zhu B, Hamdan S, Richardson C. Mechanism of sequence-specific template binding by the DNA primase of bacteriophage T7. Nucleic Acids Res. 2010;38:4372-83 pubmed publisher
    ..We present a model in which conformational changes induced during primer synthesis facilitate contact between the zinc-binding domain and the polymerase domain...
  53. Tran N, Lee S, Richardson C, Tabor S. A novel nucleotide kinase encoded by gene 1.7 of bacteriophage T7. Mol Microbiol. 2010;77:492-504 pubmed publisher
    Gene 1.7 of bacteriophage T7 confers sensitivity of both phage T7 and its host Escherichia coli to dideoxythymidine (ddT). We have purified the product of gene 1.7, gp1.7. It exists in two forms of molecular weight 22,181 and 17,782...
  54. Brieba L, Eichman B, Kokoska R, DOUBLIE S, Kunkel T, Ellenberger T. Structural basis for the dual coding potential of 8-oxoguanosine by a high-fidelity DNA polymerase. EMBO J. 2004;23:3452-61 pubmed
    ..This explains why translesion synthesis is permitted without proofreading of an 8oG.dA mismatch, thus providing insight into the high mutagenic potential of 8oG...
  55. Satapathy A, Kulczyk A, Ghosh S, van Oijen A, Richardson C. Coupling dTTP hydrolysis with DNA unwinding by the DNA helicase of bacteriophage T7. J Biol Chem. 2011;286:34468-78 pubmed publisher
    The DNA helicase encoded by gene 4 of bacteriophage T7 assembles on single-stranded DNA as a hexamer of six identical subunits with the DNA passing through the center of the toroid...
  56. Ionel A, Velazquez Muriel J, Luque D, Cuervo A, Castón J, Valpuesta J, et al. Molecular rearrangements involved in the capsid shell maturation of bacteriophage T7. J Biol Chem. 2011;286:234-42 pubmed publisher
  57. Garcia Doval C, van Raaij M. Structure of the receptor-binding carboxy-terminal domain of bacteriophage T7 tail fibers. Proc Natl Acad Sci U S A. 2012;109:9390-5 pubmed publisher
    The six bacteriophage T7 tail fibers, homo-trimers of gene product 17, are thought to be responsible for the first specific, albeit reversible, attachment to Escherichia coli lipopolysaccharide...
  58. Cheng X, Wang W, Molineux I. F exclusion of bacteriophage T7 occurs at the cell membrane. Virology. 2004;326:340-52 pubmed publisher
    The F plasmid PifA protein, known to be the cause of F exclusion of bacteriophage T7, is shown to be a membrane-associated protein. No transmembrane domains of PifA were located. In contrast, T7 gp1...
  59. Robins W, Faruque S, Mekalanos J. Coupling mutagenesis and parallel deep sequencing to probe essential residues in a genome or gene. Proc Natl Acad Sci U S A. 2013;110:E848-57 pubmed publisher
    ..Here we have applied this method to T7 bacteriophage and T7-like virus JSF7 of Vibrio cholerae...
  60. Marintcheva B, Marintchev A, Wagner G, Richardson C. Acidic C-terminal tail of the ssDNA-binding protein of bacteriophage T7 and ssDNA compete for the same binding surface. Proc Natl Acad Sci U S A. 2008;105:1855-60 pubmed publisher
    ..This mechanism prevents random binding of charged molecules to the nucleic acid-binding pocket and coordinates nucleic acid-protein and protein-protein interactions...
  61. Guo F, Liu Z, Vago F, Ren Y, Wu W, Wright E, et al. Visualization of uncorrelated, tandem symmetry mismatches in the internal genome packaging apparatus of bacteriophage T7. Proc Natl Acad Sci U S A. 2013;110:6811-6 pubmed publisher
    ..used single-particle electron cryomicroscopy to study the multilayer structure of the portal vertex of the bacteriophage T7 procapsid, the recipient of T7 DNA in packaging...
  62. Heineman R, Bull J. Testing optimality with experimental evolution: lysis time in a bacteriophage. Evolution. 2007;61:1695-709 pubmed
    ..Here we evolved lysis time in bacteriophage T7, a virus of Escherichia coli...
  63. Herrmann S, Leshem B, Lobel L, Bin H, Mendelson E, Ben Nathan D, et al. T7 phage display of Ep15 peptide for the detection of WNV IgG. J Virol Methods. 2007;141:133-40 pubmed
  64. Hu B, Margolin W, Molineux I, Liu J. The bacteriophage t7 virion undergoes extensive structural remodeling during infection. Science. 2013;339:576-9 pubmed publisher
    ..After ejection, the extended phage tail collapsed or disassembled, which allowed resealing of the infected cell membrane. These structural studies provide a detailed series of intermediates during phage infection...
  65. Crampton D, Guo S, Johnson D, Richardson C. The arginine finger of bacteriophage T7 gene 4 helicase: role in energy coupling. Proc Natl Acad Sci U S A. 2004;101:4373-8 pubmed
    The DNA helicase encoded by gene 4 of bacteriophage T7 couples DNA unwinding to the hydrolysis of dTTP...
  66. Gone S, Nicholson A. Bacteriophage T7 protein kinase: Site of inhibitory autophosphorylation, and use of dephosphorylated enzyme for efficient modification of protein in vitro. Protein Expr Purif. 2012;85:218-23 pubmed publisher
    b>Bacteriophage T7 encodes a serine/threonine-specific protein kinase that phosphorylates multiple cellular proteins during infection of Escherichia coli...
  67. Li Y, Dutta S, DOUBLIE S, Bdour H, Taylor J, Ellenberger T. Nucleotide insertion opposite a cis-syn thymine dimer by a replicative DNA polymerase from bacteriophage T7. Nat Struct Mol Biol. 2004;11:784-90 pubmed
    ..the structural basis for this, we determined crystal structures of a replicative DNA polymerase from bacteriophage T7 in complex with nucleotide substrates and a DNA template containing a cis-syn cyclobutane pyrimidine dimer (..
  68. Schlicke M, Brakmann S. Expression and purification of histidine-tagged bacteriophage T7 DNA polymerase. Protein Expr Purif. 2005;39:247-53 pubmed
    ..This phenomenon is also observed with bacteriophage T7 gene 5 protein, the phage-encoded subunit of T7 DNA polymerase, if expression is based on the T5 promoter/lac ..
  69. Akabayov B, Lee S, Akabayov S, Rekhi S, Zhu B, Richardson C. DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the zinc-binding domain. Biochemistry. 2009;48:1763-73 pubmed publisher
    Synthesis of oligoribonucleotide primers for lagging-strand DNA synthesis in the DNA replication system of bacteriophage T7 is catalyzed by the primase domain of the gene 4 helicase-primase...
  70. Bull J, Molineux I. Predicting evolution from genomics: experimental evolution of bacteriophage T7. Heredity (Edinb). 2008;100:453-63 pubmed publisher
    ..of molecular biology has been exploited in designing and interpreting experimental evolution studies with bacteriophage T7. The modest size of its genome (40 kb dsDNA) and the ease of making genetic constructs, combined with the ..
  71. Bull J, Springman R, Molineux I. Compensatory evolution in response to a novel RNA polymerase: orthologous replacement of a central network gene. Mol Biol Evol. 2007;24:900-8 pubmed