bacteriophage p1

Summary

Summary: A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE, which infects E. coli. It is the largest of the COLIPHAGES and consists of double-stranded DNA, terminally redundant, and circularly permuted.

Top Publications

  1. Chatterjee P, Mukherjee S, Shakes L, Wilson W, Coren J, Harewood K, et al. Selecting transpositions using phage P1 headful packaging: new markerless transposons for functionally mapping long-range regulatory sequences in bacterial artificial chromosomes and P1-derived artificial chromosomes. Anal Biochem. 2004;335:305-15 pubmed
    ..The procedure nevertheless offers a potential approach to map recombinogenic sequences in BACs and PACs...
  2. Bendtsen J, Nilsson A, Lehnherr H. Phylogenetic and functional analysis of the bacteriophage P1 single-stranded DNA-binding protein. J Virol. 2002;76:9695-701 pubmed
    b>Bacteriophage P1 encodes a single-stranded DNA-binding protein (SSB-P1), which shows 66% amino acid sequence identity to the SSB protein of the host bacterium Escherichia coli...
  3. Hansen A, Lehnherr H, Wang X, Mobley V, Jin D. Escherichia coli SspA is a transcription activator for bacteriophage P1 late genes. Mol Microbiol. 2003;48:1621-31 pubmed
    ..coli RNA polymerase (RNAP)-associated protein, has been reported to be essential for lytic growth of bacteriophage P1. Unlike P1 early promoters, P1 late promoters are not recognized by RNAP alone...
  4. Imam A, Patrinos G, de Krom M, Bottardi S, Janssens R, Katsantoni E, et al. Modification of human beta-globin locus PAC clones by homologous recombination in Escherichia coli. Nucleic Acids Res. 2000;28:E65 pubmed
    ..No rearrangements were detected with the exception of one highly repetitive genomic sequence when either the E.COLI: RecA- or the lambdoid phage encoded RecT and RecE-dependent recombination systems were used...
  5. Osoegawa K, Tateno M, Woon P, Frengen E, Mammoser A, Catanese J, et al. Bacterial artificial chromosome libraries for mouse sequencing and functional analysis. Genome Res. 2000;10:116-28 pubmed
    ..The low rate of chimerism, approximately 1%, and the low level of detected rearrangements support the anticipated usefulness of the BAC libraries for genome research...
  6. Lemonnier M, Ziegelin G, Reick T, Muñoz Gómez A, Díaz Orejas R, Lanka E. Bacteriophage P1 Ban protein is a hexameric DNA helicase that interacts with and substitutes for Escherichia coli DnaB. Nucleic Acids Res. 2003;31:3918-28 pubmed
    Since the ban gene of bacteriophage P1 suppresses a number of conditionally lethal dnaB mutations in Escherichia coli, it was assumed that Ban protein is a DNA helicase (DnaB analogue) that can substitute for DnaB in the host replication ..
  7. Wang Z, Engler P, Longacre A, Storb U. An efficient method for high-fidelity BAC/PAC retrofitting with a selectable marker for mammalian cell transfection. Genome Res. 2001;11:137-42 pubmed
    ..We use a BAC/PAC retrofitting vector that, on transformation into competent BAC or PAC strains, will catalyze the specific insertion of itself into BAC/PAC vectors through in vivo cre/loxP site-specific recombination...
  8. Zhang Z, Lutz B. Cre recombinase-mediated inversion using lox66 and lox71: method to introduce conditional point mutations into the CREB-binding protein. Nucleic Acids Res. 2002;30:e90 pubmed
    ..Our results indicate that this Cre- mediated inversion method is a valuable tool to introduce point mutations in the mouse genome in a regulatable manner...
  9. Derose E, Kirby T, Mueller G, Chikova A, Schaaper R, London R. Phage like it HOT: solution structure of the bacteriophage P1-encoded HOT protein, a homolog of the theta subunit of E. coli DNA polymerase III. Structure. 2004;12:2221-31 pubmed
    ..It was recently discovered that E. coli bacteriophage P1 encodes a theta homolog, named HOT...

More Information

Publications91

  1. Wang Y, Xu Z, Pierce J, Guo X. Characterization of eastern oyster (Crassostrea virginica Gmelin) chromosomes by fluorescence in situ hybridization with bacteriophage P1 clones. Mar Biotechnol (NY). 2005;7:207-14 pubmed
    ..We tested bacteriophage P1 clones for chromosomal identification in the eastern oyster Crassostrea virginica, using fluorescence in situ ..
  2. Gruenig M, Lu D, Won S, Dulberger C, Manlick A, Keck J, et al. Creating directed double-strand breaks with the Ref protein: a novel RecA-dependent nuclease from bacteriophage P1. J Biol Chem. 2011;286:8240-51 pubmed publisher
    The bacteriophage P1-encoded Ref protein enhances RecA-dependent recombination in vivo by an unknown mechanism. We demonstrate that Ref is a new type of enzyme; that is, a RecA-dependent nuclease...
  3. Ho T, Waldor M. Enterohemorrhagic Escherichia coli O157:H7 gal mutants are sensitive to bacteriophage P1 and defective in intestinal colonization. Infect Immun. 2007;75:1661-6 pubmed
    ..Thus, one way in which the O157 O antigen may contribute to EHEC intestinal colonization is to promote resistance to host-derived antimicrobial polypeptides...
  4. Liu J, Chen C, Shiomi D, Niki H, Margolin W. Visualization of bacteriophage P1 infection by cryo-electron tomography of tiny Escherichia coli. Virology. 2011;417:304-11 pubmed publisher
    b>Bacteriophage P1 has a contractile tail that targets the conserved lipopolysaccharide on the outer membrane surface of the host for initial adsorption...
  5. Lehnherr H, Bendtsen J, Preuss F, Ilyina T. Identification and characterization of the single-stranded DNA-binding protein of bacteriophage P1. J Bacteriol. 1999;181:6463-8 pubmed
    The genome of bacteriophage P1 harbors a gene coding for a 162-amino-acid protein which shows 66% amino acid sequence identity to the Escherichia coli single-stranded DNA-binding protein (SSB)...
  6. Frengen E, Zhao B, Howe S, Weichenhan D, Osoegawa K, Gjernes E, et al. Modular bacterial artificial chromosome vectors for transfer of large inserts into mammalian cells. Genomics. 2000;68:118-26 pubmed
  7. Nistala R, Sigmund C. A reliable and efficient method for deleting operational sequences in PACs and BACs. Nucleic Acids Res. 2002;30:e41 pubmed
  8. Chatterjee P, Shakes L, Srivastava D, Garland D, Harewood K, Moore K, et al. Mutually exclusive recombination of wild-type and mutant loxP sites in vivo facilitates transposon-mediated deletions from both ends of genomic DNA in PACs. Nucleic Acids Res. 2004;32:5668-76 pubmed
  9. Ioannou P, Amemiya C, Garnes J, Kroisel P, Shizuya H, Chen C, et al. A new bacteriophage P1-derived vector for the propagation of large human DNA fragments. Nat Genet. 1994;6:84-9 pubmed
    ..Similarly, no insert instability has been observed after extended culturing, for 20 clones. We conclude that the PAC cloning system will be useful in the mapping and detailed analysis of complex genomes...
  10. Siegel R, Jain R, Bradbury A. Using an in vivo phagemid system to identify non-compatible loxP sequences. FEBS Lett. 2001;505:467-73 pubmed
    The site-specific recombination system of bacteriophage P1 is composed of the Cre recombinase that recognizes a 34-bp loxP site...
  11. Chikova A, Schaaper R. The bacteriophage P1 hot gene product can substitute for the Escherichia coli DNA polymerase III {theta} subunit. J Bacteriol. 2005;187:5528-36 pubmed
    ..Putative homologs have also been found in the genomes of bacteriophage P1 and of certain conjugative plasmids...
  12. Chatterjee P, Coren J. Isolating large nested deletions in bacterial and P1 artificial chromosomes by in vivo P1 packaging of products of Cre-catalysed recombination between the endogenous and a transposed loxP site. Nucleic Acids Res. 1997;25:2205-12 pubmed
    ..These procedures should facilitate physical and functional mapping of genes and regulatory elements in these large plasmids...
  13. Łobocka M, Rose D, Plunkett G, Rusin M, Samojedny A, Lehnherr H, et al. Genome of bacteriophage P1. J Bacteriol. 2004;186:7032-68 pubmed
    ..The base content and distribution in P1 DNA indicate that replication of P1 from its plasmid origin had more impact on the base compositional asymmetries of the P1 genome than replication from the lytic origin of replication...
  14. Sternberg N. Cloning high molecular weight DNA fragments by the bacteriophage P1 system. Trends Genet. 1992;8:11-6 pubmed
    ..This review describes the background and some recent advances in cloning of high molecular weight DNA using the bacteriophage P1 system.
  15. Paul S, Summers D. ArgR and PepA, accessory proteins for XerCD-mediated resolution of ColE1 dimers, are also required for stable maintenance of the P1 prophage. Plasmid. 2004;52:63-8 pubmed
    ..We propose that ArgR and PepA are involved in Cre-lox recombination in vivo, probably by constraining the system to resolution of prophage dimers...
  16. Raina S, Missiakas D, Georgopoulos C. The rpoE gene encoding the sigma E (sigma 24) heat shock sigma factor of Escherichia coli. EMBO J. 1995;14:1043-55 pubmed
    ..by transducing strains carrying a single copy of either phtrA-lacZ or rpoHP3-lacZ fusions with mutagenized bacteriophage P1 lysates and screening for Lac- mutant colonies at 22 degrees C...
  17. Shcherbak N, Kishchenko O, Sakhno L, Komarnytsky I, Kuchuk M. Lox-dependent gene expression in transgenic plants obtained via Agrobacterium-mediated transformation. Tsitol Genet. 2013;47:21-32 pubmed
    Lox sites of the Cre/lox recombination system from bacteriophage P1 were analyzed for their ability to affect on transgene expression when inserted upstream from a gene coding sequence adjacent to the right border (RB) of T-DNA...
  18. Chaperon D. A method for the construction of in frame substitutions in operons: deletion of the essential Escherichia coli holB gene coding for a subunit of the DNA polymerase III holoenzyme. J Microbiol Methods. 2006;65:127-34 pubmed
    ..The method of the holB null construction presented in this work allows for a simplified studying of interactions between the different subunits of DNA polymerase III. ..
  19. Kuhlenbaumer G, Schirmacher A, Meuleman J, Tissir F, Del Favero J, Stogbauer F, et al. A sequence-ready BAC/PAC contig and partial transcript map of approximately 1.5 Mb in human chromosome 17q25 comprising multiple disease genes. Genomics. 1999;62:242-50 pubmed
    ..This sequence-ready PAC and BAC contig will be pivotal for the positional cloning of the HNA gene as well as other disease genes mapping to this region. ..
  20. Irobi J, Tissir F, De Jonghe P, De Vriendt E, Van Broeckhoven C, Timmerman V, et al. A clone contig of 12q24.3 encompassing the distal hereditary motor neuropathy type II gene. Genomics. 2000;65:34-43 pubmed
    ..This physical map provides a valuable resource for mapping genes and markers located within the distal HMN II region and facilitates the positional cloning of the distal HMN II gene. ..
  21. Lech P, Somia N. Retrovirus vectors. Contrib Nephrol. 2008;159:30-46 pubmed publisher
    ..Finally, we discuss potential pathologies and avenues for the optimization of the technology for gene transfer to a complex organ such as the kidney. ..
  22. Bauer A, Savelyeva L, Claas A, Praml C, Berthold F, Schwab M. Smallest region of overlapping deletion in 1p36 in human neuroblastoma: a 1 Mbp cosmid and PAC contig. Genes Chromosomes Cancer. 2001;31:228-39 pubmed
    ..deleted region shared among tumors, we assembled a physical map of the I Mbp SRO consisting predominantly of bacteriophage P1-derived artificial chromosome (PAC) clones...
  23. Lee L, Sadowski P. Identification of Cre residues involved in synapsis, isomerization, and catalysis. J Biol Chem. 2003;278:36905-15 pubmed
    The Cre protein of bacteriophage P1 is a tyrosine recombinase and catalyzes recombination via formation of a covalent protein-DNA complex and a Holliday junction intermediate...
  24. Kim M, Park J, Park H, Chung I, Park K. PAC and cosmid contig spanning the HOXA cluster on human chromosome 7p15. Somat Cell Mol Genet. 1998;24:245-8 pubmed
  25. Day C, Smilinich N, Fitzpatrick G, deJong P, Shows T, Higgins M. The imprinted domain in mouse distal Chromosome 7: reagents for mutagenesis and sequencing. Mamm Genome. 1999;10:182-5 pubmed
  26. Lieber M. Bacteriophage research: its deeper significance for science. Riv Biol. 2006;99:224-6 pubmed
  27. Schnaitman C. Phage biology: coming of age. Science. 2002;298:2329 pubmed
  28. Lehnherr H, Jensen C, Stenholm A, Dueholm A. Dual regulatory control of a particle maturation function of bacteriophage P1. J Bacteriol. 2001;183:4105-9 pubmed
    A unique arrangement of promoter elements was found upstream of the bacteriophage P1 particle maturation gene (mat)...
  29. Hao W, Xu W, Chen B, Wang P, Dong W, Li M. [Preparation and identification of phage-displayed ICAM-1-mimic peptide]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2003;19:77-9 pubmed
    ..To obtain bioactive ICAM-1 mimetic peptide...
  30. Shin J, Lin C, Lim H. Horizontal transfer of DNA methylation patterns into bacterial chromosomes. Nucleic Acids Res. 2016;44:4460-71 pubmed publisher
    ..the horizontal transfer of genes into the bacterial cytoplasm but can be transferred into chromosomes by: (i) bacteriophage P1 transduction; and (ii) transformation of extracellular synthetic DNA...
  31. MacDonald A, Lu Y, Kilbride E, Akopian A, Colloms S. PepA and ArgR do not regulate Cre recombination at the bacteriophage P1 loxP site. Plasmid. 2008;59:119-26 pubmed publisher
    In the lysogenic state, bacteriophage P1 is maintained as a low copy-number circular plasmid. Site-specific recombination at loxP by the phage-encoded Cre protein keeps P1 monomeric, thus helping to ensure stable plasmid inheritance...
  32. Burn T, Connors T, Van Raay T, Dackowski W, Millholland J, Klinger K, et al. Generation of a transcriptional map for a 700-kb region surrounding the polycystic kidney disease type 1 (PKD1) and tuberous sclerosis type 2 (TSC2) disease genes on human chromosome 16p3.3. Genome Res. 1996;6:525-37 pubmed
    ..We have initiated studies to identify transcribed sequences in this region using a bacteriophage P1 contig containing 700 kb of DNA surrounding the PKD1 and TSC2 genes...
  33. Arbing M, Handelman S, Kuzin A, Verdon G, Wang C, Su M, et al. Crystal structures of Phd-Doc, HigA, and YeeU establish multiple evolutionary links between microbial growth-regulating toxin-antitoxin systems. Structure. 2010;18:996-1010 pubmed publisher
    ..Our crystal structures of Phd-Doc from bacteriophage P1, the HigA antitoxin from Escherichia coli CFT073, and YeeU of the YeeUWV systems from E...
  34. Koma D, Yamanaka H, Moriyoshi K, Ohmoto T, Sakai K. A convenient method for multiple insertions of desired genes into target loci on the Escherichia coli chromosome. Appl Microbiol Biotechnol. 2012;93:815-29 pubmed publisher
    ..The resultant strains selectively overproduced the target aromatic compounds. Thus, the developed method is a convenient tool for the metabolic engineering of E. coli for the production of valuable compounds. ..
  35. de Boer J, Williams A, Skavdis G, Harker N, Coles M, Tolaini M, et al. Transgenic mice with hematopoietic and lymphoid specific expression of Cre. Eur J Immunol. 2003;33:314-25 pubmed
    b>Bacteriophage P1 Cre/loxP based systems can be used to manipulate the genomes ofmice in vivo and in vitro, allowing the generation of tissue-specific conditional mutants...
  36. O Keefe D, Su S, Bacich D, Horiguchi Y, Luo Y, Powell C, et al. Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene. Biochim Biophys Acta. 1998;1443:113-27 pubmed
    ..Analysis of sequence differences between non-coding regions of the two genes suggests duplication and divergence occurred 22 million years ago. ..
  37. Hwang L, Vecchiarelli A, Han Y, Mizuuchi M, Harada Y, Funnell B, et al. ParA-mediated plasmid partition driven by protein pattern self-organization. EMBO J. 2013;32:1238-49 pubmed publisher
    ..Our results support a diffusion-ratchet model, where ParB on the plasmid chases and redistributes the ParA gradient on the nucleoid, which in turn mobilizes the plasmid. ..
  38. Stanley N, Findlay K, Berks B, Palmer T. Escherichia coli strains blocked in Tat-dependent protein export exhibit pleiotropic defects in the cell envelope. J Bacteriol. 2001;183:139-44 pubmed
    ..Both phenotypes are similar to those displayed by strains carrying point mutations in the lpxC (envA) gene. The phenotype was not replicated by mutations affecting synthesis and/or activity of all known or predicted Tat substrates. ..
  39. Li L, Rao N, Kornberg A. Inorganic polyphosphate essential for lytic growth of phages P1 and fd. Proc Natl Acad Sci U S A. 2007;104:1794-9 pubmed
    ..A filamentous phage, fd, also failed to produce plaques on a mutant lawn. Although fd adsorbed to the F-pilus, its DNA failed to enter the mutant host. ..
  40. León M, Santander J, Curtiss R, Robeson J. Natural lysogenization and transduction in Salmonella enterica serovar Choleraesuis by bacteriophage P1. Res Microbiol. 2013;164:1-5 pubmed publisher
    It has been reported that bacteriophage P1 injects DNA into serovar Choleraesuis without evidence of productive infection. However, we found that P1 generates progeny and is capable of transduction in serovar Choleraesuis...
  41. Garcia Pino A, Balasubramanian S, Wyns L, Gazit E, De Greve H, MAGNUSON R, et al. Allostery and intrinsic disorder mediate transcription regulation by conditional cooperativity. Cell. 2010;142:101-11 pubmed publisher
    ..Our experiments provide the basis for understanding the mechanism of conditional cooperative regulation of transcription typical of toxin-antitoxin modules. This model may be applicable for the regulation of other biological systems. ..
  42. Wang C, Shi J, Huang Y, Cruz P, Ochoa B, Hawkins Lee B, et al. Construction of a physical and transcript map for a 1-Mb genomic region containing the urofacial (Ochoa) syndrome gene on 10q23-q24 and localization of the disease gene within two overlapping BAC clones (<360 kb). Genomics. 1999;60:12-9 pubmed
    ..Shotgun sequencing of the two BAC clones and BLASTN search of the EST databases revealed 3 other ESTs contained in the UFS critical region. These results will facilitate the cloning and identification of the UFS gene. ..
  43. Kennedy E, Nelson E, Tanaka T, Damiano J, Timp G. Live Bacterial Physiology Visualized with 5 nm Resolution Using Scanning Transmission Electron Microscopy. ACS Nano. 2016;10:2669-77 pubmed publisher
    ..On the other hand, with a cumulative dose below LD50 (and lower SNR), it was still possible to visualize the infection of E. coli by P1, showing the insertion of viral DNA within 3 s, with 5 nm resolution. ..
  44. Copeland N, Jenkins N, Court D. Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet. 2001;2:769-79 pubmed
  45. Hosoda F, Arai Y, Kitamura E, Inazawa J, Fukushima M, Tokino T, et al. A complete NotI restriction map covering the entire long arm of human chromosome 11. Genes Cells. 1997;2:345-57 pubmed
    ..A chromosome-wide NotI restriction map was constructed to understand the overall feature of the genome organization and to facilitate the positional cloning of disease genes...
  46. Kenzaka T, Tani K, Sakotani A, Yamaguchi N, Nasu M. High-frequency phage-mediated gene transfer among Escherichia coli cells, determined at the single-cell level. Appl Environ Microbiol. 2007;73:3291-9 pubmed
  47. Vollmer M, Kremer M, Ruf R, Miot S, Nothwang H, Wirth J, et al. Molecular cloning of the critical region for glomerulopathy with fibronectin deposits (GFND) and evaluation of candidate genes. Genomics. 2000;68:127-35 pubmed
    ..Although no loss-of-function mutation has been identified as yet, these data provide a basis for the examination of candidate genes for GFND and other genes for MPGN, which localize to the vicinity of the GFND region...
  48. Ioannidu S, Walter L, Dressel R, Gunther E. Physical map and expression profile of genes of the telomeric class I gene region of the rat MHC. J Immunol. 2001;166:3957-65 pubmed
    ..The data provide further molecular information on the MHC for analyzing disease susceptibility and underline the usefulness of the rat model...
  49. Coren J, Sternberg N. Construction of a PAC vector system for the propagation of genomic DNA in bacterial and mammalian cells and subsequent generation of nested deletions in individual library members. Gene. 2001;264:11-8 pubmed
    ..All library members constructed in pJCPAC-Mam1 can be directly transformed into human cells to assess function. And the deletion technology can be used to aid in delineating the boundaries of genes and other cis-acting elements...
  50. Engl C, Beek A, Bekker M, De Mattos J, Jovanovic G, Buck M. Dissipation of proton motive force is not sufficient to induce the phage shock protein response in Escherichia coli. Curr Microbiol. 2011;62:1374-85 pubmed publisher
    ..Taken together, this study suggests that not a single but likely multiple signals are needed to be integrated to induce the Psp response...
  51. Slavcev R, Funnell B. Identification and characterization of a novel allele of Escherichia coli dnaB helicase that compromises the stability of plasmid P1. J Bacteriol. 2005;187:1227-37 pubmed
    b>Bacteriophage P1 lysogenizes Escherichia coli cells as a plasmid with approximately the same copy number as the copy number of the host chromosome...
  52. Cherniavskaia A, Morozova I, Lebedeva S, Zarenkov M. [Construction of variants of Yersinia pestis EV76 (RIEG line) vaccine strain differing in antibiotic resistance spectra with stage-by-stage transduction of R-transposons]. Antibiot Khimioter. 2005;50:13-7 pubmed
  53. Yu B, Kim C. Minimization of the Escherichia coli genome using the Tn5-targeted Cre/loxP excision system. Methods Mol Biol. 2008;416:261-77 pubmed publisher
    ..coli deletion strain from which all the individual deleted regions are excised. This process will eventually yield an E. coli strain in which the genome is reduced in size and contains only regions that are essential for viability...
  54. Sengupta M, Nielsen H, Youngren B, Austin S. P1 plasmid segregation: accurate redistribution by dynamic plasmid pairing and separation. J Bacteriol. 2010;192:1175-83 pubmed publisher
  55. Redaschi N, Bickle T. Posttranscriptional regulation of EcoP1I and EcoP15I restriction activity. J Mol Biol. 1996;257:790-803 pubmed
    ..In addition, we have preliminary evidence from in vivo gene fusion studies that the res gene may code for more than one gene product...
  56. Thierauf A, Perez G, Maloy A. Generalized transduction. Methods Mol Biol. 2009;501:267-86 pubmed publisher
    ..In this chapter two of the best-studied systems - Escherichia coli-phage P1, and Salmonella enterica-phage P22 - are discussed from theoretical and practical perspectives...
  57. Gasser D, Sternberg N, Pierce J, Goldner Sauve A, Feng H, Haq A, et al. P1 and cosmid clones define the organization of 280 kb of the mouse H-2 complex containing the Cps-1 and Hsp70 loci. Immunogenetics. 1994;39:48-55 pubmed
    ..1 and BAT6 (valyl-tRNA synthetase). This refines the location of the Cps-1 locus to a 45 kb region contained in the H2-124 P1 insert...
  58. Braybrook C, Warry G, Howell G, Mandryko V, Arnason A, Bjornsson A, et al. Physical and transcriptional mapping of the X-linked cleft palate and ankyloglossia (CPX) critical region. Hum Genet. 2001;108:537-45 pubmed
    ..However, these variants have also been detected at a lower frequency on unaffected chromosomes, indicating that they are polymorphisms that are unlikely to cause the CPX phenotype...
  59. Zhang X, Lee K, Heng H, Tsui L, Parnes J, Shepherd N, et al. Isolation of P1 bacteriophage clones containing large contiguous segments of the human and mouse loci for the T-cell coreceptor molecule CD8. Genet Anal Tech Appl. 1994;11:129-39 pubmed
  60. Selbert S, Bentley D, Melton D, Rannie D, Lourenco P, Watson C, et al. Efficient BLG-Cre mediated gene deletion in the mammary gland. Transgenic Res. 1998;7:387-96 pubmed
    ..Crossing the transgenic BLG-Cre strain described here to mice harbouring other floxed alleles will facilitate the functional analysis of those genes during differentiation and development of the mammary gland...
  61. Murty V, Montgomery K, Dutta S, Bala S, Renault B, Bosl G, et al. A 3-Mb high-resolution BAC/PAC contig of 12q22 encompassing the 830-kb consensus minimal deletion in male germ cell tumors. Genome Res. 1999;9:662-71 pubmed
    ..The sequence data described in this paper have been submitted to the Genome Survey Sequence under accession nos. AQ254896-AQ254955 and AQ269251-AQ269266. Online supplementary material is available at http://www.genome.org]..
  62. Baker L, Lodge J. Multiple gene deletion in Cryptococcus neoformans using the Cre-lox system. Methods Mol Biol. 2012;845:85-98 pubmed publisher
    ..Here, we describe the adaptation of the Bacteriophage P1 Cre-loxP system for use in C...
  63. Pan X, Minegishi N, Harigae H, Yamagiwa H, Minegishi M, Akine Y, et al. Identification of human GATA-2 gene distal IS exon and its expression in hematopoietic stem cell fractions. J Biochem. 2000;127:105-12 pubmed
  64. Lehnherr H, Yarmolinsky M. Addiction protein Phd of plasmid prophage P1 is a substrate of the ClpXP serine protease of Escherichia coli. Proc Natl Acad Sci U S A. 1995;92:3274-7 pubmed
    ..This conclusion situates P1 among plasmids that elicit severe withdrawal symptoms and are able to do so because they encode both a cell toxin and an actively degraded macromolecule that blocks the synthesis or function of the toxin...
  65. Sutherland H, Pick E, Francis F, Lehrach H, Frischauf A. Mapping around the Fused locus on mouse chromosome 17. Mamm Genome. 1995;6:449-53 pubmed
    ..The candidate region has been covered to a large extent by YAC and P1 contigs, and has been partly characterized by pulsed-field gel analysis...
  66. Chatterjee P, Sternberg N. Retrofitting high molecular weight DNA cloned in P1: introduction of reporter genes, markers selectable in mammalian cells and generation of nested deletions. Genet Anal. 1996;13:33-42 pubmed
    ..1. vector, thus facilitating the use of clones from the current P.1. recombinant libraries in cellular transformation studies...
  67. Garcia Pino A, Christensen Dalsgaard M, Wyns L, Yarmolinsky M, Magnuson R, Gerdes K, et al. Doc of prophage P1 is inhibited by its antitoxin partner Phd through fold complementation. J Biol Chem. 2008;283:30821-7 pubmed publisher
    ..Moreover, Doc activates the endogenous E. coli RelE mRNA interferase but does not require this or any other known chromosomal toxin-antitoxin locus for its action in vivo...
  68. Sergeeva Z, Burova L, Tovkach F. [Introduction of transpozon Tn9 to endogenic plasmids of Erwinia carotovora during lysogenization of cells by coliphage P1]. Mikrobiol Z. 2006;68:34-9 pubmed
    ..A convenient method is offered for selecting bacterial clones carrying the plasmid pCA25::Tn9 which is based on counterselection of P1-lysogens at high concentrations of chloramphenicol - 100-120 microg/ml...
  69. Shakes L, Garland D, Srivastava D, Harewood K, Chatterjee P. Minimal cross-recombination between wild-type and loxP511 sites in vivo facilitates truncating both ends of large DNA inserts in pBACe3.6 and related vectors. Nucleic Acids Res. 2005;33:e118 pubmed
  70. Sektas M, Specht M. Limited use of the Cre/loxP recombination system in efficient production of loxP-containing minicircles in vivo. Plasmid. 2005;53:148-63 pubmed
    The Cre/loxP recombination system of bacteriophage P1 is one of the most powerful tools in genome engineering...
  71. Sauer B. Inducible gene targeting in mice using the Cre/lox system. Methods. 1998;14:381-92 pubmed
    ..switches and for speeding the creation of gene-modified animals, is the Cre site-specific DNA recombinase of bacteriophage P1. Precise DNA rearrangements and genetic switches can be efficiently generated in a straightforward manner ..
  72. Grimm L, Holinski Feder E, Teodoridis J, Scheffer B, Schindelhauer D, Meitinger T, et al. Analysis of the human GDNF gene reveals an inducible promoter, three exons, a triplet repeat within the 3'-UTR and alternative splice products. Hum Mol Genet. 1998;7:1873-86 pubmed
    ..Furthermore, fibroblast growth factor 2, tetradecanoyl 12-phorbol acetate, an inflammatory agent, and cAMP increase promoter activity, suggesting that GDNF transcriptional regulation is a target of exogenous signals...
  73. Min Kim J, Young Choi J, Sun Kim M, Chang Kim S. In vivo excision and amplification of large human genomic segments using the Cre/loxP-and large T antigen/SV40 ori-mediated machinery. J Biotechnol. 2004;110:227-33 pubmed
    ..in vivo excision and amplification system in human BJAB cells was devised by combining the Cre/loxP system of bacteriophage P1 and the large T antigen/SV40 ori system of Simian virus 40...
  74. Engert J, Dore C, Mercier J, Ge B, Betard C, Rioux J, et al. Autosomal recessive spastic ataxia of Charlevoix-Saguenay (ARSACS): high-resolution physical and transcript map of the candidate region in chromosome region 13q11. Genomics. 1999;62:156-64 pubmed
    ..Six BAC/PAC clones form a contig between D13S232 and D13S787 for sequencing within the ARSACS critical region...
  75. Chang W, Chang N, Lin S, Wu C, Wu F. Tissue-specific cancer-related serpin gene cluster at human chromosome band 3q26. Genes Chromosomes Cancer. 2000;29:240-55 pubmed
    ..This finding indicates that the distance between human PI14 and PI12 is approximately 100 kb, and hence we speculate that other tissue-specific cancer-related serpin genes are likely to reside within this 3q26.1 cluster region...
  76. Williams M, Ouyang T, Flickinger M. Glutathione S-transferase-sspA fusion binds to E. coli RNA polymerase and complements delta sspA mutation allowing phage P1 replication. Biochem Biophys Res Commun. 1994;201:123-7 pubmed
    b>Bacteriophage P1 is unable to form plaques on E. coli hosts lacking a functional sspA gene...
  77. Higgins M, Day C, Smilinich N, Ni L, Cooper P, Nowak N, et al. Contig maps and genomic sequencing identify candidate genes in the usher 1C locus. Genome Res. 1998;8:57-68 pubmed
    ..Based on their map location, these loci represent candidate disease loci for USH1C. The NEFA gene was assessed as the USH1C locus by the sequencing of an amplified NEFA cDNA from an USH1C patient; however, no mutations were detected...
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    ..Dre-mediated recombination in mammalian cells showed that, like Cre, no host bacterial proteins are required for efficient Dre-mediated site-specific DNA recombination...
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    ..The proximity of IDD to the balanced translocation breakpoint and its position within the shortest region of deletion overlap indicate that this gene may have a role, along with other genes, in the CATCH22 haploinsufficiency syndromes...
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