sperm midpiece

Summary

Summary: The middle piece of the spermatozoon is a highly organized segment consisting of MITOCHONDRIA, the outer dense fibers and the core microtubular structure.

Top Publications

  1. Downing Meisner A, Klaus A, O Leary M. Sperm head morphology in 36 species of artiodactylans, perissodactylans, and cetaceans (Mammalia). J Morphol. 2005;263:179-202 pubmed
    ..2002] J Struct Biol 138:187-198) was detected in artiodactylans, and its shape is described for the species examined...
  2. Firman R, Simmons L. Sperm midpiece length predicts sperm swimming velocity in house mice. Biol Lett. 2010;6:513-6 pubmed publisher
    ..We conducted in vitro sperm velocity assays on males from established selection lines, and found that sperm midpiece size was the only phenotypic predictor of sperm swimming velocity.
  3. Ferramosca A, Albani D, Coppola L, Zara V. Varicocele Negatively Affects Sperm Mitochondrial Respiration. Urology. 2015;86:735-9 pubmed publisher
    ..05) in mitochondrial respiratory activity in comparison to the control group. A 32% increase (P <.05) in sperm midpiece defects and a 41% decrease (P <.05) in sperm concentration and motility were also observed...
  4. Tomasek O, Albrechtova J, Němcová M, Opatová P, Albrecht T. Trade-off between carotenoid-based sexual ornamentation and sperm resistance to oxidative challenge. Proc Biol Sci. 2017;284: pubmed publisher
    ..Shortening of the sperm midpiece following oxidative challenge further suggests that redox homeostasis may constrain sperm morphometry...
  5. Fisher H, Jacobs Palmer E, Lassance J, Hoekstra H. The genetic basis and fitness consequences of sperm midpiece size in deer mice. Nat Commun. 2016;7:13652 pubmed publisher
    ..Our findings suggest that rapid evolution of reproductive traits can occur through cell type-specific changes to ubiquitously expressed genes and have an important effect on fitness. ..
  6. Meng X, Gong Y, Huang J, Zeng Y, Quan S, Zhong Y. [Impact of sperm midpiece morphology on embryo development following intracytoplasmic morphologically selected sperm injection]. Nan Fang Yi Ke Da Xue Xue Bao. 2016;36:255-9 pubmed
    To evaluate the impact of sperm midpiece morphology observed under high-power microscope on embryo development following intracytoplasmic morphologically selected sperm injection...
  7. Angrimani D, Losano J, Lúcio C, Veiga G, Landim F, Nichi M, et al. Cytoplasmic droplet acting as a mitochondrial modulator during sperm maturation in dogs. Anim Reprod Sci. 2017;181:50-56 pubmed publisher
    ..In the corpus segment, the migration of the CD along the sperm midpiece provides a high mitochondrial activity and the onset of sperm motility...
  8. Swegen A, Lambourne S, Aitken R, Gibb Z. Rosiglitazone Improves Stallion Sperm Motility, ATP Content, and Mitochondrial Function. Biol Reprod. 2016;95:107 pubmed
    ..AMPK localized to the sperm midpiece, and its phosphorylation, was increased in rosiglitazone-treated spermatozoa...
  9. Zhu J, Yang W. Ultrastructure of the mature spermatozoon of the bivalve Estellarca olivacea (Mollusca: Bivalvia: Arcidae) and its phylogenetic implications. Acta Biol Hung. 2009;60:27-34 pubmed publisher
    ..Whether the particular acrosome, subacrosomal space, or the highly condensed nucleus might be adaptations of high fertilization rate in the particular environment of this species is discussed...

More Information

Publications63

  1. Liu Y, Chen G, Lu L, Sun H, Guo Q, Xue K, et al. RNASET2 in human spermatozoa and seminal plasma: a novel relevant indicator for asthenozoospermia. Andrology. 2013;1:75-84 pubmed publisher
    ..05). In conclusion, higher expression of RNASET2 in the semen of asthenozoospermia individuals may contribute to sperm motility impairment...
  2. Bian X, Gandahi J, Liu Y, Yang P, Liu Y, Zhang L, et al. The ultrastructural characteristics of the spermatozoa stored in the cauda epididymidis in Chinese soft-shelled turtle Pelodiscus sinensis during the breeding season. Micron. 2013;44:202-9 pubmed publisher
    ..Thus, in the present study we hypothesized that the cauda epididymidis might be involved in the sperm maturation in this species...
  3. Zhang X, Yao K, Xiong C. [A comparative study of sperm morphology evaluation criteria by the fifth and fourth editions of WHO Laboratory Manual]. Zhonghua Nan Ke Xue. 2011;17:989-92 pubmed
  4. Woolley D, Carter D, Tilly G. Compliance in the neck structures of the guinea pig spermatozoon, as indicated by rapid freezing and electron microscopy. J Anat. 2008;213:336-41 pubmed publisher
  5. Shi L, Nascimento J, Chandsawangbhuwana C, Botvinick E, Berns M. An automatic system to study sperm motility and energetics. Biomed Microdevices. 2008;10:573-83 pubmed publisher
    ..This system should have application in both the human fertility clinic and in animal husbandry...
  6. Escalier D. Arrest of flagellum morphogenesis with fibrous sheath immaturity of human spermatozoa. Andrologia. 2006;38:54-60 pubmed
    ..These anomalies raise the question of the mechanisms that lead to impairment of both the annulus relocation and the deposit of proteins on the fibrous sheath during spermiogenesis...
  7. Soler C, de Monserrat J, Gutierrez R, Nunez J, Nunez M, Sancho M, et al. Use of the Sperm-Class Analyser for objective assessment of human sperm morphology. Int J Androl. 2003;26:262-70 pubmed
  8. Nipper R, Chennothukuzhi V, Tutuncu L, Williams C, Gerton G, Moss S. Differential RNA expression and polyribosome loading of alternative transcripts of the Akap4 gene in murine spermatids. Mol Reprod Dev. 2005;70:397-405 pubmed
    ..Antisense RNA transcripts complementary to both the Akap82 and Fsc1 mRNAs were present, suggesting that translatability may be regulated by these RNAs...
  9. Chao H, Chung C, Pan H, Liao P, Kuo P, Hsu C. Protein tyrosine phosphatase non-receptor type 14 is a novel sperm-motility biomarker. J Assist Reprod Genet. 2011;28:851-61 pubmed publisher
    ..To understand the molecular basis of sperm-motility and to identify related novel motility biomarkers...
  10. Erkan M, Sousa M, Baldaia L. Comparative ultracytochemical study of the acrosome in four different Veneroida species from the Turkish coast. J Submicrosc Cytol Pathol. 2002;34:125-30 pubmed
    ..gallina and the apical and outer regions in P. rudis. In midpiece, only C. glaucum showed a positive silver reaction at the centriolar fossa...
  11. Zhen L, Wang L, Fu J, Li Y, Zhao N, Li X. Hexavalent chromium affects sperm motility by influencing protein tyrosine phosphorylation in the midpiece of boar spermatozoa. Reprod Toxicol. 2016;59:66-79 pubmed publisher
    ..These results suggest that chromium affects boar sperm motility by impairing tyrosine phosphorylation in the midpiece of sperm by blocking the cAMP/PKA pathway in boar sperm in vitro. ..
  12. Harayama H. Roles of intracellular cyclic AMP signal transduction in the capacitation and subsequent hyperactivation of mouse and boar spermatozoa. J Reprod Dev. 2013;59:421-30 pubmed
  13. Guerin J, Cornut Thibaut A, Giscard Destaing S, Pouvreau S, Guillemin Y, Aouacheria A. Subcellular dynamics of the maternal cell death regulator BCL2L10 in human preimplantation embryos. Hum Reprod. 2013;28:729-39 pubmed publisher
    ..What is the expression status and subcellular localization of the maternally expressed Bcl-2 family member, BCL2L10, in early human embryos of diverse developmental stages and quality?..
  14. Lefievre L, Nash K, Mansell S, Costello S, Punt E, Correia J, et al. 2-APB-potentiated channels amplify CatSper-induced Ca(2+) signals in human sperm. Biochem J. 2012;448:189-200 pubmed publisher
    ..We propose that 2-APB-sensitive channels amplify [Ca2+]i elevation induced by progesterone (and other CatSper agonists), amplifying, propagating and providing spatio-temporal complexity in [Ca2+]i signals of human sperm...
  15. Spehr M, Schwane K, Riffell J, Barbour J, Zimmer R, Neuhaus E, et al. Particulate adenylate cyclase plays a key role in human sperm olfactory receptor-mediated chemotaxis. J Biol Chem. 2004;279:40194-203 pubmed
    ..Particulate AC is therefore required for induction of hOR17-4-mediated human sperm behavior and represents a promising target for future design of contraceptive drugs...
  16. Flores Herrera H, Acuña Hernández D, Rivera Rebolledo J, González Jiménez M, Rodas Martínez A, Swanson W. Effect of increasing trypsin concentrations on seminal coagulum dissolution and sperm parameters in spider monkeys (Ateles geoffroyi). Theriogenology. 2012;78:612-9 pubmed publisher
  17. Kraus M, Ticha M, Zelezna B, Peknicova J, Jonáková V. Characterization of human seminal plasma proteins homologous to boar AQN spermadhesins. J Reprod Immunol. 2005;65:33-46 pubmed
    ..However, localization of hSA proteins on the sperm tail and neck suggests that their biological role differs from that of boar AQN spermadhesins located on the sperm head...
  18. Sahin E, Petrunkina A, Ekhlasi Hundrieser M, Hettel C, Waberski D, Harrison R, et al. Fibronectin type II-module proteins in the bovine genital tract and their putative role in cell volume control during sperm maturation. Reprod Fertil Dev. 2009;21:479-88 pubmed
    ..While the protein had no effect on cauda spermatozoa, it caused caput spermatozoa to swell more in response to hypotonic stress, similarly to untreated cauda spermatozoa...
  19. Lipke C, Meinecke Tillmann S, Meyer W, Meinecke B. Preparation and ultrastructure of spermatozoa from green poison frogs, Dendrobates auratus, following hormonal induced spermiation (Amphibia, Anura, Dendrobatidae). Anim Reprod Sci. 2009;113:177-86 pubmed publisher
    ..g. amphibian phylogeny without killing frogs threatened with extinction but allows investigations in the field of assisted reproduction and male fertility for example in conservation programs for endangered amphibians...
  20. Psenicka M, Alavi S, Rodina M, Gela D, Nebesarova J, Linhart O. Morphology and ultrastructure of Siberian sturgeon (Acipenser baerii) spermatozoa using scanning and transmission electron microscopy. Biol Cell. 2007;99:103-15 pubmed
    ..In contrast with teleost fish, chondrostean spermatozoa have a head with a distinct acrosome, whereas other structures, such as a midpiece and a single flagellum, are present in spermatozoa of most species...
  21. Gwo J, Chiu J, Lin C, Su Y, Yu S. Spermatozoal ultrastructure of four Sparidae fishes: Acanthopagrus berda, Acanthopagrus australis, Lagodon rhomboids and Archosargus probatocephus. Tissue Cell. 2005;37:109-15 pubmed
    ..Variation in the depths of the nuclear fossa and mitochondria number is substantial in these four Sparidae species. This study provide useful systematic characters to the existing knowledge of comparative spermatology of Sparidae...
  22. Birkhead T, Pellatt E, Brekke P, Yeates R, Castillo Juarez H. Genetic effects on sperm design in the zebra finch. Nature. 2005;434:383-7 pubmed
    ..These results have important implications for the evolution of sperm in other taxa...
  23. Gwo J, Kuo M, Chiu J, Cheng H. Ultrastructure of Pagrus major and Rhabdosargus sarba spermatozoa (Perciformes: Sparidae: Sparinae). Tissue Cell. 2004;36:141-7 pubmed
    ..It is concluded that the spermatozoa of both species are of primitive type, and they are distinguished by several unique features which may provide useful systematic characteristics...
  24. Talarczyk Desole J, Kotwicka M, Jendraszak M, Pawelczyk L, Murawski M, Jędrzejczak P. Sperm midpiece apoptotic markers: impact on fertilizing potential in in vitro fertilization and intracytoplasmic sperm injection. Hum Cell. 2016;29:67-75 pubmed publisher
    ..2, p = 0.85 for PST and r = 0.1, p = 0.51 for active c3). The two apoptotic markers localized in the sperm midpiece area may affect their function not only by decreasing basic andrologic parameters but also by reducing the ..
  25. Kashir J, Sermondade N, Sifer C, Oo S, Jones C, Mounce G, et al. Motile sperm organelle morphology evaluation-selected globozoospermic human sperm with an acrosomal bud exhibits novel patterns and higher levels of phospholipase C zeta. Hum Reprod. 2012;27:3150-60 pubmed publisher
    ..Does motile sperm organelle morphology examination (MSOME) affect levels and localization patterns of the oocyte activation factor phospholipase C zeta (PLC?) in globozoospermic sperm with and without an acrosomal bud?..
  26. Evans J. Patterns of genetic variation and covariation in ejaculate traits reveal potential evolutionary constraints in guppies. Heredity (Edinb). 2011;106:869-75 pubmed publisher
    ..Nevertheless, these prospective analyses point to potential constraints on ejaculate evolution and may explain the high level of phenotypic variability in ejaculate traits in this species...
  27. Brito L, Sertich P, Stull G, Rives W, Knobbe M. Sperm ultrastructure, morphometry, and abnormal morphology in American black bears (Ursus americanus). Theriogenology. 2010;74:1403-13 pubmed publisher
    ..The morphology of abnormal sperm and the underlying ultrastructural defects were similar to that in other large domestic animals thus suggesting similar underlying pathogenesis of specific sperm defects and similar effects on fertility...
  28. Lesich K, Zhang Z, Kelsch C, Ponichter K, Strauss J, Lindemann C. Functional deficiencies and a reduced response to calcium in the flagellum of mouse sperm lacking SPAG16L. Biol Reprod. 2010;82:736-44 pubmed publisher
    ..This deficit in motor function, and an increased rigidity of the midpiece region which we detected in the passive flagella, together can explain the observed motility characteristics of the Spag16L-null sperm...
  29. Bohring C, Klepper L, Krause W. Localization of binding sites of naturally occurring antisperm antibodies on human spermatozoa by immunofluorescence. Andrologia. 2004;36:286-90 pubmed
    ..Immunofluorescence appears to be a valuable tool in the diagnosis of immune infertility, in particular when impairment of the acrosome activity is suggested...
  30. Diallo A, Diarra M, Sow A, Mbodj M, Afoutou J. [Ultrastructural aspects of the spermatozoon abnormalities on infertile men in Senegal]. Dakar Med. 2007;52:17-22 pubmed
  31. Morales C, Badran H, El Alfy M, Men H, Zhang H, Martin DeLeon P. Cytoplasmic localization during testicular biogenesis of the murine mRNA for Spam1 (PH-20), a protein involved in acrosomal exocytosis. Mol Reprod Dev. 2004;69:475-82 pubmed
  32. Fehr J, Meyer D, Widmayer P, Borth H, Ackermann F, Wilhelm B, et al. Expression of the G-protein alpha-subunit gustducin in mammalian spermatozoa. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2007;193:21-34 pubmed
  33. Fabrega A, Puigmulé M, Dacheux J, Bonet S, Pinart E. Glycocalyx characterisation and glycoprotein expression of Sus domesticus epididymal sperm surface samples. Reprod Fertil Dev. 2012;24:619-30 pubmed publisher
    ..The sperm glycocalyx modifications observed in the present study provide an insight into the molecular modifications associated with epididymal maturation, which may be correlated with the degree of maturation of ejaculated spermatozoa...
  34. Ounjai P, Kim K, Lishko P, Downing K. Three-dimensional structure of the bovine sperm connecting piece revealed by electron cryotomography. Biol Reprod. 2012;87:73 pubmed
    ..On the other hand, our video recordings of the bull sperm movement show little bending of the head with respect to the tail, so it appears that there may be normally little strain within the connecting piece...
  35. Reunov A. [Problem of terminology in characteristics of spermatozoa of metazoa]. Ontogenez. 2005;36:403-21 pubmed
    ..The terms "primitive spermatozoon", "modified spermatozoon", and "aberrant spermatozoon" will become more universal if the method of their combined utilization is applied...
  36. Kehr S, Malinouski M, Finney L, Vogt S, Labunskyy V, Kasaikina M, et al. X-ray fluorescence microscopy reveals the role of selenium in spermatogenesis. J Mol Biol. 2009;389:808-18 pubmed publisher
    ..Overall, the use of XFM allowed visualization of tissue and cellular Se and provided important insights in the role of this and other trace elements in spermatogenesis...
  37. Aguiar O, Giaretta A, Recco Pimentel S. The sperm of Hylodinae species (Anura, Leptodactylidae): ultrastructural characteristics and their relevance to interspecific taxonomic relationships. J Biosci. 2006;31:379-88 pubmed
    ..This distinctive flagellar condition expands the already known variability in sperm structure within the Leptodactylidae...
  38. Zhang X, Ibrahim E, de Rivero Vaccari J, Lotocki G, Aballa T, Dietrich W, et al. Involvement of the inflammasome in abnormal semen quality of men with spinal cord injury. Fertil Steril. 2013;99:118-24 pubmed publisher
    ..To investigate inflammasome components and end-product cytokines in semen of SCI and control subjects...
  39. Navarro B, Miki K, Clapham D. ATP-activated P2X2 current in mouse spermatozoa. Proc Natl Acad Sci U S A. 2011;108:14342-7 pubmed publisher
    ..However, fertility of P2rx2(-/-) males declines with frequent mating over days, suggesting that P2X2 receptor adds a selection advantage under these conditions...
  40. Garda A, Costa G, Colli G, Báo S. Spermatozoa of Pseudinae (Amphibia, Anura, Hylidae), with a test of the hypothesis that sperm ultrastructure correlates with reproductive modes in anurans. J Morphol. 2004;261:196-205 pubmed
    ..Therefore, support for the correlation between sperm ultrastructure and fertilization environments in anurans is, at best, equivocal...
  41. Feiden S, Stypa H, Wolfrum U, Wegener G, Kamp G. A novel pyruvate kinase (PK-S) from boar spermatozoa is localized at the fibrous sheath and the acrosome. Reproduction. 2007;134:81-95 pubmed
    ..In the principal piece, both polyclonal anti-PK-M1 and anti-PK-S were found at the fibrous sheath. Our results suggest that PK-S is a major component in the structural organization of glycolysis in boar spermatozoa...
  42. Kankavi O, Ata A, Celik Ozenci C, Sati L, Ciftcioglu M, Demir R, et al. Presence and subcellular localizations of surfactant proteins A and D in human spermatozoa. Fertil Steril. 2008;90:1904-9 pubmed publisher
    ..To investigate the presence of surfactant protein-A (SP-A); molecular weight 34 kDa and surfactant protein-D (SP-D); and molecular weight 43 kDa in human spermatozoa...
  43. Kalahanis J, Rousso D, Kourtis A, Mavromatidis G, Makedos G, Panidis D. Round-headed spermatozoa in semen specimens from fertile and subfertile men. J Reprod Med. 2002;47:489-93 pubmed
    ..To investigate the frequency of round-headed, or acrosomeless, spermatozoa, determine the percentage and evaluate the possible correlation with other semen parameters...
  44. Terada Y, Nakamura S, Morita J, Tachibana M, Morito Y, Ito K, et al. Use of Mammalian eggs for assessment of human sperm function: molecular and cellular analyses of fertilization by intracytoplasmic sperm injection. Am J Reprod Immunol. 2004;51:290-3 pubmed
    ..Egg activation, sperm decondensation and sperm centorosomal function were examined in sperm from fertile men and infertile patients...
  45. Abid S, Sagare Patil V, Gokral J, Modi D. Cellular ontogeny of RBMY during human spermatogenesis and its role in sperm motility. J Biosci. 2013;38:85-92 pubmed
    ..One such possible function of RBMY could be its involvement in sperm motility...
  46. Tripepi S, Jamieson B, Brunelli E. Ultrastructure of the spermatid of Caprimulgus europaeus Linnaeus 1758, the European nightjar (Aves; Caprimulgidae), with phylogenetic implications. J Morphol. 2006;267:1157-64 pubmed
    ..The structure of the spermatid and spermatozoon is consistent with placement of the Caprimulgidae near the Psittacidae, but is less supportive of close proximity to the Apodidae, from DNA-DNA hybridization and some other analyses...
  47. Plante G, Thérien I, Manjunath P. Characterization of recombinant murine binder of sperm protein homolog 1 and its role in capacitation. Biol Reprod. 2012;87:20, 1-11 pubmed publisher
    ..These results show that murine epididymal BSPH1 shares many biochemical and functional characteristics with BSP proteins secreted by seminal vesicles of ungulates, and suggest that it might play a similar role in sperm functions...
  48. Ramió Lluch L, Fernández Novell J, Pena A, Bucci D, Rigau T, Rodriguez Gil J. "In vitro" capacitation and subsequent acrosome reaction are related to changes in the expression and location of midpiece actin and mitofusin-2 in boar spermatozoa. Theriogenology. 2012;77:979-88 pubmed publisher
  49. Maricchiolo G, Genovese L, Laura R, Micale V, Muglia U. Fine structure of spermatozoa in the common pandora (Pagellus erythrinus Linnaeus, 1758) (Perciformes, Sparidae). Histol Histopathol. 2004;19:1237-40 pubmed publisher
    ..According to the morphological classification proposed by Mattei (1970), the sperm of pandora belongs to a "type I" designation, like that of the other Sparid fish...
  50. Asquith K, Kitchener A, Kay D. Immunisation of the male tammar wallaby (Macropus eugenii) with spermatozoa elicits epididymal antigen-specific antibody secretion and compromised fertilisation rate. J Reprod Immunol. 2006;69:127-47 pubmed
    ..This study demonstrates that a contraceptive vaccine targeting sperm antigens has potential for fertility control in male macropods...
  51. Ugajin T, Terada Y, Hasegawa H, Nabeshima H, Suzuki K, Yaegashi N. The shape of the sperm midpiece in intracytoplasmic morphologically selected sperm injection relates sperm centrosomal function. J Assist Reprod Genet. 2010;27:75-81 pubmed publisher
    To evaluate whether the morphology of the sperm midpiece observed by high magnification microscopy relates to sperm centrosomal function.
  52. Li K, Jin J, Chen W, Shi Q, Ni Y, Roldan E. Secretory phospholipase A2 group IID Is involved in progesterone-induced acrosomal exocytosis of human spermatozoa. J Androl. 2012;33:975-83 pubmed
    ..In conclusion, sPLA(2)-IID is present at the head and midpiece in the human sperm, and activation of such sPLA(2)-IID seems to be involved in AE. Therefore, sPLA(2)-IID isoform plays a functional role during the AE in human sperm...
  53. Cummins J. Mitochondria in reproduction. Reprod Biomed Online. 2004;8:14-5 pubmed
  54. Scheltinga D, Wilkinson M, Jamieson B, Oommen O. Ultrastructure of the mature spermatozoa of caecilians (Amphibia: Gymnophiona). J Morphol. 2003;258:179-92 pubmed