tropoelastin

Summary

Summary: A salt-soluble precursor of elastin. Lysyl oxidase is instrumental in converting it to elastin in connective tissue.

Top Publications

  1. Merrilees M, Lemire J, Fischer J, Kinsella M, Braun K, Clowes A, et al. Retrovirally mediated overexpression of versican v3 by arterial smooth muscle cells induces tropoelastin synthesis and elastic fiber formation in vitro and in neointima after vascular injury. Circ Res. 2002;90:481-7 pubmed
    ..We now report that V3-overexpressing ASMCs exhibit significantly increased expression of tropoelastin and increased formation of elastic fibers in long-term cell cultures...
  2. Muiznieks L, Jensen S, Weiss A. Structural changes and facilitated association of tropoelastin. Arch Biochem Biophys. 2003;410:317-23 pubmed
    Circular dichroism studies of tropoelastin secondary structure show 4+/-1% alpha-helix in aqueous solutions...
  3. Wise S, Mithieux S, Raftery M, Weiss A. Specificity in the coacervation of tropoelastin: solvent exposed lysines. J Struct Biol. 2005;149:273-81 pubmed
    b>Tropoelastin protein monomers associate by coacervation and are cross-linked in vivo to form elastin macro-assemblies...
  4. Cain S, Baldwin A, Mahalingam Y, Raynal B, Jowitt T, Shuttleworth C, et al. Heparan sulfate regulates fibrillin-1 N- and C-terminal interactions. J Biol Chem. 2008;283:27017-27 pubmed publisher
    ..by exons 59-62 near the fibrillin-1 C terminus are novel conformation-dependent high affinity heparin and tropoelastin binding sites...
  5. Wagenseil J, Mecham R. New insights into elastic fiber assembly. Birth Defects Res C Embryo Today. 2007;81:229-40 pubmed publisher
  6. Baldock C, Oberhauser A, Ma L, Lammie D, Siegler V, Mithieux S, et al. Shape of tropoelastin, the highly extensible protein that controls human tissue elasticity. Proc Natl Acad Sci U S A. 2011;108:4322-7 pubmed publisher
    Elastin enables the reversible deformation of elastic tissues and can withstand decades of repetitive forces. Tropoelastin is the soluble precursor to elastin, the main elastic protein found in mammals...
  7. Huang R, Merrilees M, Braun K, Beaumont B, Lemire J, Clowes A, et al. Inhibition of versican synthesis by antisense alters smooth muscle cell phenotype and induces elastic fiber formation in vitro and in neointima after vessel injury. Circ Res. 2006;98:370-7 pubmed
    ..versican production, induced a flattened morphology, reduced cell proliferation and migration, increased tropoelastin synthesis, increased elastin binding protein (S-Gal/EBP), and increased deposition of elastic fibers in long-..
  8. Szabo Z, Levi Minzi S, Christiano A, Struminger C, Stoneking M, Batzer M, et al. Sequential loss of two neighboring exons of the tropoelastin gene during primate evolution. J Mol Evol. 1999;49:664-71 pubmed
    Previous evidence has demonstrated the absence of exons 34 and 35 within the 3' end of the human tropoelastin (ELN) gene...
  9. Xu C, Zarins C, Glagov S. Gene expression of tropoelastin is enhanced in the aorta proximal to the coarctation in rabbits. Exp Mol Pathol. 2002;72:115-23 pubmed
    ..in the aortic wall in response to acute elevation of blood pressure, we studied the aortic gene expression of tropoelastin in a rabbit midthoracic aortic coarctation model...

More Information

Publications84

  1. Wachi H, Nonaka R, Sato F, Shibata Sato K, Ishida M, Iketani S, et al. Characterization of the molecular interaction between tropoelastin and DANCE/fibulin-5. J Biochem. 2008;143:633-9 pubmed publisher
    ..The present experiments were carried out to characterize the molecular interaction between fibulin-5 and tropoelastin. Our data showed that the divalent cations of Ca(2+), Ba(2+) and Mg(2+) significantly enhanced the binding of ..
  2. El Hallous E, Sasaki T, Hubmacher D, Getie M, Tiedemann K, Brinckmann J, et al. Fibrillin-1 interactions with fibulins depend on the first hybrid domain and provide an adaptor function to tropoelastin. J Biol Chem. 2007;282:8935-46 pubmed
    ..Formation of ternary complexes between fibrillin-1, fibulins, and tropoelastin demonstrated that fibulin-2 and -5 but much less fibulin-4, are able to act as molecular adaptors between ..
  3. Tamburro A, Bochicchio B, Pepe A. Dissection of human tropoelastin: exon-by-exon chemical synthesis and related conformational studies. Biochemistry. 2003;42:13347-62 pubmed
    Polypeptide sequences encoding the single exons of human tropoelastin were synthesized and their conformations were studied in different solvents and at different temperatures by CD and (1)H NMR...
  4. Tsuruga E, Irie K, Sakakura Y, Yajima T. Expression of fibrillins and tropoelastin by human gingival and periodontal ligament fibroblasts in vitro. J Periodontal Res. 2002;37:23-8 pubmed
    ..the major components of microfibrils involved in elastogenesis, we investigated the synthesis of fibrillins and tropoelastin in the conditioned medium of HGF and HPLF...
  5. Miao M, Cirulis J, Lee S, Keeley F. Structural determinants of cross-linking and hydrophobic domains for self-assembly of elastin-like polypeptides. Biochemistry. 2005;44:14367-75 pubmed
    ..To achieve the required structural durability of the elastic matrix, the elastin monomer, tropoelastin, undergoes ordered assembly into a covalently cross-linked, fibrillar polymeric structure...
  6. Gheduzzi D, Guerra D, Bochicchio B, Pepe A, Tamburro A, Quaglino D, et al. Heparan sulphate interacts with tropoelastin, with some tropoelastin peptides and is present in human dermis elastic fibers. Matrix Biol. 2005;24:15-25 pubmed
    ..with normal elastic fibers in human dermis and that isolated HS chains interact in vitro with recombinant tropoelastin and with peptides encoded by distinct exons of the human tropoelastin gene (EDPs)...
  7. Mariani T, Reed J, Shapiro S. Expression profiling of the developing mouse lung: insights into the establishment of the extracellular matrix. Am J Respir Cell Mol Biol. 2002;26:541-8 pubmed
    ..These data should serve as a resource for the pulmonary research community and assist in deciphering the molecular mechanisms governing normal lung development as well as those involved in aberrant developmental pathology. ..
  8. Miao M, Bruce A, Bhanji T, Davis E, Keeley F. Differential expression of two tropoelastin genes in zebrafish. Matrix Biol. 2007;26:115-24 pubmed
    ..Elastin is synthesized as a monomer, tropoelastin, but is rapidly transformed into its final polymeric form in the extracellular matrix...
  9. Rock M, Cain S, Freeman L, Morgan A, Mellody K, Marson A, et al. Molecular basis of elastic fiber formation. Critical interactions and a tropoelastin-fibrillin-1 cross-link. J Biol Chem. 2004;279:23748-58 pubmed
    ..binding of two overlapping fibrillin-1 fragments (encoded by central exons 18-25 and 24-30) to tropoelastin, which, in microfibrils, map to an exposed "arms" feature adjacent to the beads...
  10. Choudhury R, McGovern A, Ridley C, Cain S, Baldwin A, Wang M, et al. Differential regulation of elastic fiber formation by fibulin-4 and -5. J Biol Chem. 2009;284:24553-67 pubmed publisher
    ..We have determined how they interact with tropoelastin, lysyl oxidase, and fibrillin-1, thereby revealing how they differentially regulate assembly...
  11. Taddese S, Weiss A, Jahreis G, Neubert R, Schmelzer C. In vitro degradation of human tropoelastin by MMP-12 and the generation of matrikines from domain 24. Matrix Biol. 2009;28:84-91 pubmed publisher
    ..b>Tropoelastin is the precursor of the cross-linked elastin and its expression is triggered by elastin-degrading factors as a ..
  12. Thomassin L, Werneck C, Broekelmann T, Gleyzal C, Hornstra I, Mecham R, et al. The Pro-regions of lysyl oxidase and lysyl oxidase-like 1 are required for deposition onto elastic fibers. J Biol Chem. 2005;280:42848-55 pubmed
    ..Ligand blot and mammalian two-hybrid assays confirmed an interaction between tropoelastin and the pro-regions of both LOX and LOXL...
  13. DiCamillo S, Carreras I, Panchenko M, Stone P, Nugent M, Foster J, et al. Elastase-released epidermal growth factor recruits epidermal growth factor receptor and extracellular signal-regulated kinases to down-regulate tropoelastin mRNA in lung fibroblasts. J Biol Chem. 2002;277:18938-46 pubmed
    ..with transient, peaking at 30 min, induction of c-Fos and sustained, observed at 24-48 h, decrease of tropoelastin mRNA levels in NE-challenged cells...
  14. Choi J, Bergdahl A, Zheng Q, Starcher B, Yanagisawa H, Davis E. Analysis of dermal elastic fibers in the absence of fibulin-5 reveals potential roles for fibulin-5 in elastic fiber assembly. Matrix Biol. 2009;28:211-20 pubmed publisher
    ..lower levels of mature cross-linked elastin in the Fbln5(-/-) dermis, however, gene expression levels for tropoelastin and fibrillin-1, the major elastic fiber components, were unaffected...
  15. Hu Q, Loeys B, Coucke P, De Paepe A, Mecham R, Choi J, et al. Fibulin-5 mutations: mechanisms of impaired elastic fiber formation in recessive cutis laxa. Hum Mol Genet. 2006;15:3379-86 pubmed
    ..Purified recombinant fibulin-5 with either mutation showed reduced affinity for tropoelastin in solid-phase binding assays...
  16. DiCamillo S, Yang S, Panchenko M, Toselli P, Naggar E, Rich C, et al. Neutrophil elastase-initiated EGFR/MEK/ERK signaling counteracts stabilizing effect of autocrine TGF-beta on tropoelastin mRNA in lung fibroblasts. Am J Physiol Lung Cell Mol Physiol. 2006;291:L232-43 pubmed
    ..soluble epidermal growth factor receptor (EGFR) ligands and initiates EGFR/MEK/ERK signaling to downregulate tropoelastin mRNA in neonatal rat lung fibroblasts (DiCamillo SJ, Carreras I, Panchenko MV, Stone PJ, Nugent MA, Foster JA, ..
  17. Clarke A, Wise S, Cain S, Kielty C, Weiss A. Coacervation is promoted by molecular interactions between the PF2 segment of fibrillin-1 and the domain 4 region of tropoelastin. Biochemistry. 2005;44:10271-81 pubmed
    In forming elastic fibers, microfibrils act as the scaffold sites for depositing the elastin precursor tropoelastin. We examined key binding interactions that promote massive tropoelastin association through coacervation...
  18. Nonaka R, Onoue S, Wachi H, Sato F, Urban Z, Starcher B, et al. DANCE/fibulin-5 promotes elastic fiber formation in a tropoelastin isoform-dependent manner. Clin Biochem. 2009;42:713-21 pubmed publisher
    ..interactions among elastin, fibrillin-1, and fibulin-5 in the extracellular space and the maturation of tropoelastin using retinal pigment epithelial cells (ARPE-19)...
  19. Wachi H, Sato F, Murata H, Nakazawa J, Starcher B, Seyama Y. Development of a new in vitro model of elastic fiber assembly in human pigmented epithelial cells. Clin Biochem. 2005;38:643-53 pubmed
    We developed an in vitro model of elastic fiber assembly that provides a comparison of the efficiency of different tropoelastin molecules to organize into fibers.
  20. Waterhouse A, Yin Y, Wise S, Bax D, McKenzie D, Bilek M, et al. The immobilization of recombinant human tropoelastin on metals using a plasma-activated coating to improve the biocompatibility of coronary stents. Biomaterials. 2010;31:8332-40 pubmed publisher
    ..We previously demonstrated a plasma-activated coating (PAC) that covalently bound recombinant human tropoelastin (TE), a major regulator of vascular cells in vivo, to enhance endothelial cell interactions...
  21. McKenna K, Hinds M, Sarao R, Wu P, Maslen C, Glanville R, et al. Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials. Acta Biomater. 2012;8:225-33 pubmed publisher
    ..the structural and mechanical properties and the vascular cell compatibility of electrospun recombinant human tropoelastin (rTE) were evaluated as a potential vascular graft support matrix...
  22. Keane F, Clarke A, Foster T, Weiss A. The N-terminal A domain of Staphylococcus aureus fibronectin-binding protein A binds to tropoelastin. Biochemistry. 2007;46:7226-32 pubmed publisher
    ..Here we show that region A of FnBPA (rAFnBPA) binds to recombinant human tropoelastin. Binding occurs to three separate truncates of tropoelastin, encompassing domains 2-18, 17-27, and 27-36, ..
  23. Tsuruga E, Yajima T, Irie K. Induction of fibulin-5 gene is regulated by tropoelastin gene, and correlated with tropoelastin accumulation in vitro. Int J Biochem Cell Biol. 2004;36:395-400 pubmed
    ..examined the relationship between the gene expression of fibulin-5 and the gene expression and accumulation of tropoelastin by comparing elastin-producing cells (human gingival fibroblasts) with non-elastin-producing cells (human ..
  24. Mariani T, Arikan M, Pierce R. Fibroblast tropoelastin and alpha-smooth-muscle actin expression are repressed by particulate-activated macrophage-derived tumor necrosis factor-alpha in experimental silicosis. Am J Respir Cell Mol Biol. 1999;21:185-92 pubmed
    ..Reinitiated tropoelastin expression was identified throughout the lung in fibroblasts expressing alpha-smooth-muscle actin, whereas ..
  25. Tu Y, Weiss A. Glycosaminoglycan-mediated coacervation of tropoelastin abolishes the critical concentration, accelerates coacervate formation, and facilitates spherule fusion: implications for tropoelastin microassembly. Biomacromolecules. 2008;9:1739-44 pubmed publisher
    Elastogenesis and elastin repair depend on the secretion of tropoelastin from the cell, yet cellular production is low in the many biological systems that have been studied...
  26. Seo J, Lee S, Youn C, Choi H, Rhie G, Cho K, et al. Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skin in vivo. J Invest Dermatol. 2001;116:915-9 pubmed
    ..In this study, it was demonstrated that ultraviolet irradiation induced tropoelastin mRNA expression in the keratinocytes of human skin in vivo and also in cultured human keratinocytes by in situ ..
  27. Hu X, Wang X, Rnjak J, Weiss A, Kaplan D. Biomaterials derived from silk-tropoelastin protein systems. Biomaterials. 2010;31:8121-31 pubmed publisher
    A structural protein blend system based on silkworm silk fibroin and recombinant human tropoelastin is described...
  28. Yeo G, Keeley F, Weiss A. Coacervation of tropoelastin. Adv Colloid Interface Sci. 2011;167:94-103 pubmed publisher
    The coacervation of tropoelastin represents the first major stage of elastic fiber assembly...
  29. Pierce R, Deak S, Stolle C, Boyd C. Heterogeneity of rat tropoelastin mRNA revealed by cDNA cloning. Biochemistry. 1990;29:9677-83 pubmed
    ..gt11 library constructed from poly(A+) RNA isolated from aortic tissue of neonatal rats was screened for rat tropoelastin cDNAs. The first screen, utilizing a human tropoelastin cDNA clone, provided rat tropoelastin cDNAs spanning 2...
  30. Yang S, Nugent M, Panchenko M. EGF antagonizes TGF-beta-induced tropoelastin expression in lung fibroblasts via stabilization of Smad corepressor TGIF. Am J Physiol Lung Cell Mol Physiol. 2008;295:L143-51 pubmed publisher
    ..reported that neutrophil elastase (NE) downregulates transforming growth factor-beta (TGF-beta)-maintained tropoelastin mRNA levels in lung fibroblasts through transactivation of the epidermal growth factor (EGF) receptor (EGFR)/..
  31. Sato F, Wachi H, Ishida M, Nonaka R, Onoue S, Urban Z, et al. Distinct steps of cross-linking, self-association, and maturation of tropoelastin are necessary for elastic fiber formation. J Mol Biol. 2007;369:841-51 pubmed
    Elastic fibers play an important role in the characteristic resilience of many tissues. The assembly of tropoelastin into a fibrillar matrix is a complex stepwise process and the deposition and cross-linking of tropoelastin are believed ..
  32. Sasaki T, Göhring W, Miosge N, Abrams W, Rosenbloom J, Timpl R. Tropoelastin binding to fibulins, nidogen-2 and other extracellular matrix proteins. FEBS Lett. 1999;460:280-4 pubmed
    Elastic fibers in vessel walls and other tissues consist of cross-linked tropoelastin in association with several microfibrillar proteins...
  33. Hinek A, Smith A, Cutiongco E, Callahan J, Gripp K, Weksberg R. Decreased elastin deposition and high proliferation of fibroblasts from Costello syndrome are related to functional deficiency in the 67-kD elastin-binding protein. Am J Hum Genet. 2000;66:859-72 pubmed
    ..obtained from patients with Costello syndrome did not assemble elastic fibers, despite an adequate synthesis of tropoelastin and normal deposition of the microfibrillar scaffold...
  34. Reinboth B, Hanssen E, Cleary E, Gibson M. Molecular interactions of biglycan and decorin with elastic fiber components: biglycan forms a ternary complex with tropoelastin and microfibril-associated glycoprotein 1. J Biol Chem. 2002;277:3950-7 pubmed
    ..dermatan sulfate proteoglycans biglycan and decorin have been investigated with the elastic fiber components, tropoelastin, fibrillin-containing microfibrils, and microfibril-associated glycoproteins (MAGP) 1 and 2...
  35. Le Brun A, Chow J, Bax D, Nelson A, Weiss A, James M. Molecular orientation of tropoelastin is determined by surface hydrophobicity. Biomacromolecules. 2012;13:379-86 pubmed publisher
    b>Tropoelastin is the precursor of the extracellular protein elastin and is utilized in tissue engineering and implant technology by adapting the interface presented by surface-bound tropoelastin...
  36. Lin Y, Lee D, McDonough S, Nicholson L, Sharma Y, Chang Y. Repeated domains of leptospira immunoglobulin-like proteins interact with elastin and tropoelastin. J Biol Chem. 2009;284:19380-91 pubmed publisher
    ..In this study, we report that Leptospira can be immobilized by human tropoelastin (HTE) or elastin from different tissues, including lung, skin, and blood vessels, and that Lig proteins can ..
  37. Vrhovski B, Weiss A. Biochemistry of tropoelastin. Eur J Biochem. 1998;258:1-18 pubmed
  38. Dyksterhuis L, Baldock C, Lammie D, Wess T, Weiss A. Domains 17-27 of tropoelastin contain key regions of contact for coacervation and contain an unusual turn-containing crosslinking domain. Matrix Biol. 2007;26:125-35 pubmed
    The central region of tropoelastin including domains 19-25 of human tropoelastin forms a hot-spot for contacts during the inter-molecular association of tropoelastin by coacervation [Wise, S.G., Mithieux, S.M., Raftery, M.J. and Weiss, A...
  39. Kozel B, Wachi H, Davis E, Mecham R. Domains in tropoelastin that mediate elastin deposition in vitro and in vivo. J Biol Chem. 2003;278:18491-8 pubmed
    ..To identify domains in the tropoelastin molecule important for the assembly process, we utilized an in vitro assembly model to map sequences within ..
  40. Broekelmann T, Kozel B, Ishibashi H, Werneck C, Keeley F, Zhang L, et al. Tropoelastin interacts with cell-surface glycosaminoglycans via its COOH-terminal domain. J Biol Chem. 2005;280:40939-47 pubmed
    ..and cell biological approach, we have identified a cell interaction site at the carboxyl terminus of tropoelastin. Cell interactions with the COOH-terminal sequence are not through the elastin-binding protein (EBP67) because ..
  41. Cain S, Baldock C, Gallagher J, Morgan A, Bax D, Weiss A, et al. Fibrillin-1 interactions with heparin. Implications for microfibril and elastic fiber assembly. J Biol Chem. 2005;280:30526-37 pubmed
    ..cell attachment, but heparin and MAGP-1 competed for binding to the fibrillin-1 N terminus, and heparin and tropoelastin competed for binding to a central fibrillin-1 sequence...
  42. Clarke A, Arnspang E, Mithieux S, Korkmaz E, Braet F, Weiss A. Tropoelastin massively associates during coacervation to form quantized protein spheres. Biochemistry. 2006;45:9989-96 pubmed
    b>Tropoelastin, the precursor of elastin, undergoes a rapid monomer to multimer association in an inverse temperature transition. This association culminates in the rapid formation of stable, optically distinct droplets of tropoelastin...
  43. Trask T, Trask B, Ritty T, Abrams W, Rosenbloom J, Mecham R. Interaction of tropoelastin with the amino-terminal domains of fibrillin-1 and fibrillin-2 suggests a role for the fibrillins in elastic fiber assembly. J Biol Chem. 2000;275:24400-6 pubmed
    Alignment of tropoelastin molecules during the process of elastogenesis is thought to require fibrillin-containing microfibrils...
  44. Kozel B, Rongish B, Czirok A, Zach J, Little C, Davis E, et al. Elastic fiber formation: a dynamic view of extracellular matrix assembly using timer reporters. J Cell Physiol. 2006;207:87-96 pubmed
    ..the dynamics of elastic fiber assembly, mammalian cells were transfected with a cDNA construct encoding bovine tropoelastin in frame with the Timer reporter...
  45. Hucthagowder V, Morava E, Kornak U, Lefeber D, Fischer B, Dimopoulou A, et al. Loss-of-function mutations in ATP6V0A2 impair vesicular trafficking, tropoelastin secretion and cell survival. Hum Mol Genet. 2009;18:2149-65 pubmed publisher
    ..Immunostaining of ARCL2 cells showed the accumulation of tropoelastin (TE) in the Golgi and in large, abnormal intracellular and extracellular aggregates...
  46. Chen Z, Seo J, Kim Y, Lee S, Kim K, Cho K, et al. Heat modulation of tropoelastin, fibrillin-1, and matrix metalloproteinase-12 in human skin in vivo. J Invest Dermatol. 2005;124:70-8 pubmed
    ..In this study, we investigated the effects of heat on the expression of tropoelastin and fibrillin-1, two main components of elastic fibers, and on matrix metalloproteinase (MMP)-12, the most ..
  47. Cirulis J, Keeley F. Kinetics and morphology of self-assembly of an elastin-like polypeptide based on the alternating domain arrangement of human tropoelastin. Biochemistry. 2010;49:5726-33 pubmed publisher
    ..Both tropoelastin (TE), the full-length monomeric form of elastin, and elastin-like polypeptides (ELPs), based on sequences and ..
  48. Tsuruga E, Irie K, Sakakura Y, Yajima T. Tropoelastin expression by periodontal fibroblasts. J Dent Res. 2002;81:198-202 pubmed
    ..We examined gene expression and accumulation of tropoelastin in the cell-matrix layers of human gingival fibroblasts (HGF) and periodontal ligament fibroblasts (HPLF) in ..
  49. Mariencheck M, Davis E, Zhang H, Ramirez F, Rosenbloom J, Gibson M, et al. Fibrillin-1 and fibrillin-2 show temporal and tissue-specific regulation of expression in developing elastic tissues. Connect Tissue Res. 1995;31:87-97 pubmed
    ..these possibilities, the expression of fibrillin-1 and fibrillin-2 was compared with expression of MAGP and tropoelastin in two elastogenic tissues that undergo different developmental programs...
  50. Jensen S, Reinhardt D, Gibson M, Weiss A. Protein interaction studies of MAGP-1 with tropoelastin and fibrillin-1. J Biol Chem. 2001;276:39661-6 pubmed
    ..To investigate the interaction of MAGP-1 with tropoelastin and fibrillin-1, we expressed human MAGP-1 as a T7-tag fusion protein in Escherichia coli...
  51. Yeo G, Baldock C, Tuukkanen A, Roessle M, Dyksterhuis L, Wise S, et al. Tropoelastin bridge region positions the cell-interactive C terminus and contributes to elastic fiber assembly. Proc Natl Acad Sci U S A. 2012;109:2878-83 pubmed publisher
    The tropoelastin monomer undergoes stages of association by coacervation, deposition onto microfibrils, and cross-linking to form elastic fibers...
  52. Bax D, Rodgers U, Bilek M, Weiss A. Cell adhesion to tropoelastin is mediated via the C-terminal GRKRK motif and integrin alphaVbeta3. J Biol Chem. 2009;284:28616-23 pubmed publisher
    Elastin fibers are predominantly composed of the secreted monomer tropoelastin. This protein assembly confers elasticity to all vertebrate elastic tissues including arteries, lung, skin, vocal folds, and elastic cartilage...
  53. Wu W, Vrhovski B, Weiss A. Glycosaminoglycans mediate the coacervation of human tropoelastin through dominant charge interactions involving lysine side chains. J Biol Chem. 1999;274:21719-24 pubmed
    Following cellular secretion into the extracellular matrix, tropoelastin is transported, deposited, and cross-linked to make elastin...
  54. Yin Y, Wise S, Nosworthy N, Waterhouse A, Bax D, Youssef H, et al. Covalent immobilisation of tropoelastin on a plasma deposited interface for enhancement of endothelialisation on metal surfaces. Biomaterials. 2009;30:1675-81 pubmed publisher
    ..We further demonstrated the attachment of tropoelastin, an extracellular matrix protein critical to the correct arrangement and function of vasculature...
  55. Bochicchio B, Pepe A. Role of polyproline II conformation in human tropoelastin structure. Chirality. 2011;23:694-702 pubmed
    In this review, we present a comprehensive overview of the molecular studies on human tropoelastin domains accomplished by Tamburro and co-workers in the last decade...
  56. Heinz A, Jung M, Duca L, Sippl W, Taddese S, Ihling C, et al. Degradation of tropoelastin by matrix metalloproteinases--cleavage site specificities and release of matrikines. FEBS J. 2010;277:1939-56 pubmed publisher
    ..elastin-degrading diseases, the aim of this study was to investigate the degradation of the natural substrate tropoelastin by the elastinolytic matrix metalloproteinases MMP-7, MMP-9, and MMP-12 and to compare the cleavage site ..
  57. Lee P, Bax D, Bilek M, Weiss A. A novel cell adhesion region in tropoelastin mediates attachment to integrin ?V?5. J Biol Chem. 2014;289:1467-77 pubmed publisher
    b>Tropoelastin protein monomers assemble to form elastin. Cellular integrin ?V?3 binds RKRK at the C-terminal tail of tropoelastin...
  58. Davis E, Broekelmann T, Ozawa Y, Mecham R. Identification of tropoelastin as a ligand for the 65-kD FK506-binding protein, FKBP65, in the secretory pathway. J Cell Biol. 1998;140:295-303 pubmed
    The folding and trafficking of tropoelastin is thought to be mediated by intracellular chaperones, although the identity and role of any tropoelastin chaperone remain to be determined...
  59. Ostuni A, Bochicchio B, Armentano M, Bisaccia F, Tamburro A. Molecular and supramolecular structural studies on human tropoelastin sequences. Biophys J. 2007;93:3640-51 pubmed
    ..Previously, polypeptide sequences encoded by different exons of the human tropoelastin gene have been analyzed for their ability to coacervate and to self-assemble...
  60. Wachi H, Sato F, Nakazawa J, Nonaka R, Szabo Z, Urban Z, et al. Domains 16 and 17 of tropoelastin in elastic fibre formation. Biochem J. 2007;402:63-70 pubmed
    ..b>Tropoelastin lacking domains 16-17 (Delta16-17) was synthesized efficiently and secreted by transfected retinal pigment ..
  61. Chung M, Miao M, Stahl R, Chan E, Parkinson J, Keeley F. Sequences and domain structures of mammalian, avian, amphibian and teleost tropoelastins: Clues to the evolutionary history of elastins. Matrix Biol. 2006;25:492-504 pubmed publisher
    b>Tropoelastin is the monomeric form of elastin, a polymeric extracellular matrix protein responsible for properties of extensibility and elastic recoil in connective tissues of most vertebrates...
  62. Tamburro A, Pepe A, Bochicchio B, Quaglino D, Ronchetti I. Supramolecular amyloid-like assembly of the polypeptide sequence coded by exon 30 of human tropoelastin. J Biol Chem. 2005;280:2682-90 pubmed
    ..similar to those shown by amyloid networks, is shown by the polypeptide sequence encoded by exon 30 of human tropoelastin. To better understand the molecular properties of this sequence to give amyloid fibers, we used CD, NMR, and ..
  63. Dyksterhuis L, Carter E, Mithieux S, Weiss A. Tropoelastin as a thermodynamically unfolded premolten globule protein: The effect of trimethylamine N-oxide on structure and coacervation. Arch Biochem Biophys. 2009;487:79-84 pubmed publisher
    b>Tropoelastin is the monomer building block of the biopolymer elastin, which is responsible for elasticity in arteries, lung and skin...
  64. Chen Q, Zhang T, Roshetsky J, Ouyang Z, Essers J, Fan C, et al. Fibulin-4 regulates expression of the tropoelastin gene and consequent elastic-fibre formation by human fibroblasts. Biochem J. 2009;423:79-89 pubmed publisher
    ..analysis showed that knockdown of fibulin-4 expression was accompanied by reduced expression of tropoelastin mRNA. Further analysis showed that this decrease was caused by transcriptional down-regulation of tropoelastin...
  65. Toonkool P, Jensen S, Maxwell A, Weiss A. Hydrophobic domains of human tropoelastin interact in a context-dependent manner. J Biol Chem. 2001;276:44575-80 pubmed
    b>Tropoelastin is the soluble precursor of elastin, the major component of the extracellular elastic fiber...
  66. Kobayashi N, Kostka G, Garbe J, Keene D, Bachinger H, Hanisch F, et al. A comparative analysis of the fibulin protein family. Biochemical characterization, binding interactions, and tissue localization. J Biol Chem. 2007;282:11805-16 pubmed
    ..Binding to tropoelastin was strong for fibulin-2 and -5, moderate for fibulin-4 and -1, and relatively weak for fibulin-3...
  67. Tamburro A, Pepe A, Bochicchio B. Localizing alpha-helices in human tropoelastin: assembly of the elastin "puzzle". Biochemistry. 2006;45:9518-30 pubmed
    Polyalanine cross-linking domains encoded by exons 6, 15, 17, 19, 21, 23, 25, 27, 29, 31 of human tropoelastin were synthesized, and their conformations were studied in different solutions and at different temperatures by CD and (1)H NMR...
  68. McLaughlin P, Chen Q, Horiguchi M, Starcher B, Stanton J, Broekelmann T, et al. Targeted disruption of fibulin-4 abolishes elastogenesis and causes perinatal lethality in mice. Mol Cell Biol. 2006;26:1700-9 pubmed
    ..However, expression of tropoelastin or lysyl oxidase mRNA was unaffected in fibulin-4-/- mice...
  69. Callewaert B, Renard M, Hucthagowder V, Albrecht B, Hausser I, Blair E, et al. New insights into the pathogenesis of autosomal-dominant cutis laxa with report of five ELN mutations. Hum Mutat. 2011;32:445-55 pubmed publisher
    ..Mutant protein expression in fibroblast cultures impaired deposition of tropoelastin onto microfibril-containing fibers, and enhanced tropoelastin coacervation and globule formation leading to ..
  70. Faury G, Garnier S, Weiss A, Wallach J, Fulop T, Jacob M, et al. Action of tropoelastin and synthetic elastin sequences on vascular tone and on free Ca2+ level in human vascular endothelial cells. Circ Res. 1998;82:328-36 pubmed
    ..we have compared the action of different concentrations (10(-13) to 10(-7) mol/L) of recombinant human tropoelastin, eight synthetic elastin peptides, and a control peptide (VPVGGA) on both rat aortic ring tension and [Ca2+]i ..
  71. Tsuruga E, Irie K, Yajima T. Gene expression and accumulation of fibrillin-1, fibrillin-2, and tropoelastin in cultured periodontal fibroblasts. J Dent Res. 2002;81:771-5 pubmed
    ..to determine the gene expression of fibrillin-1 and fibrillin-2 (the major components of microfibrils) and of tropoelastin. In addition, we assessed the degree of accumulation of these proteins in the extracellular matrix...
  72. Tsuruga E, Yajima T, Irie K. Microfibril-associated glycoprotein-1 and fibrillin-2 are associated with tropoelastin deposition in vitro. Int J Biochem Cell Biol. 2005;37:120-9 pubmed
    Elastic system fibers consist of microfibrils and tropoelastin. During development, microfibrils act as a template on which tropoelastin is deposited...
  73. Urban Z, Gao J, Pope F, Davis E. Autosomal dominant cutis laxa with severe lung disease: synthesis and matrix deposition of mutant tropoelastin. J Invest Dermatol. 2005;124:1193-9 pubmed
    ..In addition to the normal 68 kDa tropoelastin (TE) protein, an abnormal, 120 kDa polypeptide was detected in the proband and her affected daughter in a CL ..
  74. Miao M, Stahl R, Petersen L, Reintsch W, Davis E, Keeley F. Characterization of an unusual tropoelastin with truncated C-terminus in the frog. Matrix Biol. 2009;28:432-41 pubmed publisher
    b>Tropoelastin is the monomeric form of elastin, a major polymeric protein of the extracellular elastic matrix of vertebrate tissues with properties of extensibility and elastic recoil...
  75. Cirulis J, Bellingham C, Davis E, Hubmacher D, Reinhardt D, Mecham R, et al. Fibrillins, fibulins, and matrix-associated glycoprotein modulate the kinetics and morphology of in vitro self-assembly of a recombinant elastin-like polypeptide. Biochemistry. 2008;47:12601-13 pubmed publisher
    ..Recent data suggest the complex interaction of tropoelastin, the monomeric form of elastin, with a number of other elastic matrix-associated proteins, including fibrillins,..