autolysis

Summary

Summary: The spontaneous disintegration of tissues or cells by the action of their own autogenous enzymes.

Top Publications

  1. Rout M, Hosur R. Fluctuating partially native-like topologies in the acid denatured ensemble of autolysis resistant HIV-1 protease. Arch Biochem Biophys. 2009;482:33-41 pubmed publisher
    ..report here NMR characterization of the acid-denatured state of a mutant of HIV-1 protease, designed to prevent autolysis (Q7K, L33I, L63I) and to prevent cysteine oxidation (C67A and C95A)...
  2. Douillard A, Galbes O, Rossano B, Vernus B, Bonnieu A, Candau R, et al. Time course in calpain activity and autolysis in slow and fast skeletal muscle during clenbuterol treatment. Can J Physiol Pharmacol. 2011;89:117-25 pubmed publisher
    ..purpose, for 21 days we followed the time course of the calpain activity and of the ubiquitous calpain 1 and 2 autolysis, as well as muscle remodeling in the extensor digitorum longus (EDL) and soleus muscles of male Wistar rats ..
  3. Rezaie A, Sun M, Gailani D. Contributions of basic amino acids in the autolysis loop of factor XIa to serpin specificity. Biochemistry. 2006;45:9427-33 pubmed
    The autolysis loops (amino acids 143-154, chymotrypsinogen numbering) of plasma serine proteases play key roles in determining the specificity of protease inhibition by plasma serpins...
  4. Zhao J, Fleet G. Degradation of DNA during the autolysis of Saccharomyces cerevisiae. J Ind Microbiol Biotechnol. 2003;30:175-82 pubmed
    The autolysis of yeast cells has practical implications in the production of fermented foods and beverages and flavourants for food processing...
  5. Rey M, Davies P. The protease core of the muscle-specific calpain, p94, undergoes Ca2+-dependent intramolecular autolysis. FEBS Lett. 2002;532:401-6 pubmed
    ..Isolation of the intact 94-kDa enzyme has been difficult to achieve due to its rapid autolysis, and uncertainty has arisen over its Ca2+-dependence for activity...
  6. Robertson G, Zhao J, Desai B, Coleman W, Nicas T, Gilmour R, et al. Vancomycin tolerance induced by erythromycin but not by loss of vncRS, vex3, or pep27 function in Streptococcus pneumoniae. J Bacteriol. 2002;184:6987-7000 pubmed
    ..tolerance to vancomycin at various doses in multiple media and showed wild-type sensitivity to other classes of autolysis-inducing antibiotics...
  7. Pocsi I, Leiter E, Kwon N, Shin K, Kwon G, Pusztahelyi T, et al. Asexual sporulation signalling regulates autolysis of Aspergillus nidulans via modulating the chitinase ChiB production. J Appl Microbiol. 2009;107:514-23 pubmed publisher
    ..Elucidation of the regulation of ChiB production in Aspergillus nidulans...
  8. Penyige A, Matko J, Deák E, Bodnar A, Barabás G. Depolarization of the membrane potential by beta-lactams as a signal to induce autolysis. Biochem Biophys Res Commun. 2002;290:1169-75 pubmed
    The effect of beta-lactam antibiotics that are known to inhibit cell wall biosynthesis and induce cell wall autolysis on the electrophysiological state of the plasma membrane in Streptomyces griseus was studied...
  9. Botkjaer K, Fogh S, Bekes E, Chen Z, Blouse G, Jensen J, et al. Targeting the autolysis loop of urokinase-type plasminogen activator with conformation-specific monoclonal antibodies. Biochem J. 2011;438:39-51 pubmed publisher
    ..In the present study we characterize the so-called autolysis loop in the catalytic domain of uPA as a potential inhibitory target...

More Information

Publications62

  1. Romao S, Memmi G, Oggioni M, Trombe M. LuxS impacts on LytA-dependent autolysis and on competence in Streptococcus pneumoniae. Microbiology. 2006;152:333-41 pubmed
    ..found to be related on the one hand to down-regulation of competence, and on the other hand to attenuation of autolysis in cultures entering stationary phase...
  2. Emri T, Molnar Z, Veres T, Pusztahelyi T, Dudás G, Pocsi I. Glucose-mediated repression of autolysis and conidiogenesis in Emericella nidulans. Mycol Res. 2006;110:1172-8 pubmed
    Glucose-mediated repression of autolysis and sporulation was studied in submerged Emericellanidulans (anam. Aspergillus nidulans) cultures. Null mutation of the creA gene, which encodes the major carbon catabolite repressor CreA in E...
  3. Kovács Z, Szarka M, Kovacs S, Boczonádi I, Emri T, Abe K, et al. Effect of cell wall integrity stress and RlmA transcription factor on asexual development and autolysis in Aspergillus nidulans. Fungal Genet Biol. 2013;54:1-14 pubmed publisher
    ..regulated the expression of glucanase (ANID_00245, engA) and chitinase (chiB, chiA) genes, which promoted the autolysis of the cultures and also modulated the pellet sizes...
  4. Lametsch R, Lonergan S, Huff Lonergan E. Disulfide bond within mu-calpain active site inhibits activity and autolysis. Biochim Biophys Acta. 2008;1784:1215-21 pubmed publisher
    ..In the present study the influence of oxidation on activity and autolysis of mu-calpain was examined...
  5. Maleszewski J, Lu J, Fox Talbot K, Halushka M. Robust immunohistochemical staining of several classes of proteins in tissues subjected to autolysis. J Histochem Cytochem. 2007;55:597-606 pubmed
    ..paraffin-embedded human tissue arrays containing 16 tissues each were created to evaluate 48 hr of autolysis in a warm or cold environment...
  6. Tomita Y, Nihira M, Ohno Y, Sato S. Ultrastructural changes during in situ early postmortem autolysis in kidney, pancreas, liver, heart and skeletal muscle of rats. Leg Med (Tokyo). 2004;6:25-31 pubmed
    ..The effect of lysosomes and hydrolytic enzymes was not as great as previous findings...
  7. Li H, Thompson V, Goll D. Effects of autolysis on properties of mu- and m-calpain. Biochim Biophys Acta. 2004;1691:91-103 pubmed
    Although the biochemical changes that occur during autolysis of mu- and m-calpain are well characterized, there have been few studies on properties of the autolyzed calpain molecules themselves...
  8. Hickey R, Ross R, Hill C. Controlled autolysis and enzyme release in a recombinant lactococcal strain expressing the metalloendopeptidase enterolysin A. Appl Environ Microbiol. 2004;70:1744-8 pubmed
    ..lytic enzyme enterolysin A (EntL), produced by Enterococcus faecalis strain DPC5280, to elicit the controlled autolysis of starter lactococci. EntL, a cell wall metalloendopeptidase secreted by some E...
  9. Taveau M, Bourg N, Sillon G, Roudaut C, Bartoli M, Richard I. Calpain 3 is activated through autolysis within the active site and lyses sarcomeric and sarcolemmal components. Mol Cell Biol. 2003;23:9127-35 pubmed
    ..that the Capn3 activation mechanism is similar to the universal activation of caspases and corresponds to an autolysis within the active site of the protease...
  10. Molina R, Gonzalez A, Stelter M, Perez Dorado I, Kahn R, Morales M, et al. Crystal structure of CbpF, a bifunctional choline-binding protein and autolysis regulator from Streptococcus pneumoniae. EMBO Rep. 2009;10:246-51 pubmed publisher
    ..The carboxy-terminal module is involved in cell wall binding and the amino-terminal module is crucial for inhibition of the autolytic LytC muramidase, providing a regulatory function for pneumococcal autolysis.
  11. Tweedie D, Brossi A, Chen D, Ge Y, Bailey J, Yu Q, et al. Neurine, an acetylcholine autolysis product, elevates secreted amyloid-beta protein precursor and amyloid-beta peptide levels, and lowers neuronal cell viability in culture: a role in Alzheimer's disease?. J Alzheimers Dis. 2006;10:9-16 pubmed
    ..Neurine (vinyl-trimethyl-ammonium hydroxide) is a breakdown product of ACh, consequent to autolysis and is an organic poison found in cadavre brain...
  12. Yoshida R, Kuwahara Arai K, Baba T, Cui L, Richardson J, Hiramatsu K. Physiological and molecular analysis of a mecA-negative Staphylococcus aureus clinical strain that expresses heterogeneous methicillin resistance. J Antimicrob Chemother. 2003;51:247-55 pubmed
    ..The strain was characteristically a non-producer of autolytic enzyme, though the phenotype, which was lost easily, was not directly correlated with methicillin resistance...
  13. Farkas V, Takeo K, Maceková D, Ohkusu M, Yoshida S, Sipiczki M. Secondary cell wall formation in Cryptococcus neoformans as a rescue mechanism against acid-induced autolysis. FEMS Yeast Res. 2009;9:311-20 pubmed publisher
    ..The cell lysis and the formation of the secondary cell walls could be suppressed by buffering the growth medium between pH 4.5 and 6.5...
  14. Chaplin M, Barlow N, Ryder S, Simmons M, Spencer Y, Hughes R, et al. Evaluation of the effects of controlled autolysis on the immunodetection of PrP(Sc) by immunoblotting and immunohistochemistry from natural cases of scrapie and BSE. Res Vet Sci. 2002;72:37-43 pubmed
    ..One aliquot was frozen at -70 degrees C, the others were subjected to one of four autolysis regimes at 3 or 7 days at 25 degrees C or 37 degrees C...
  15. Debeer S, Baron T, Bencsik A. Immunohistochemistry of PrPsc within bovine spongiform encephalopathy brain samples with graded autolysis. J Histochem Cytochem. 2001;49:1519-24 pubmed
    ..Tissue autolysis, often unavoidable in routine field cases, is not compatible with histological examination of the brain even ..
  16. Nery L, Moreira C, Cestari T, Taga R, Damante J. Postmortem acinar autolysis in rat sublingual gland: a morphometric study. J Appl Oral Sci. 2010;18:509-14 pubmed
    ..The morphometric analysis of the volume density (Vv) and total volume (Vt) of intact (ia) and autolyzed (aa) acini was performed under light microscopy using a Zeiss II integration grid with 100 symmetrically distributed points...
  17. Pozzi N, Chen R, Chen Z, Bah A, Di Cera E. Rigidification of the autolysis loop enhances Na(+) binding to thrombin. Biophys Chem. 2011;159:6-13 pubmed publisher
    ..Here we report how replacement of the flexible autolysis loop of human thrombin with the homologous rigid domain of the murine enzyme results in a drastic (up to 10-fold)..
  18. Maki H, Yamaguchi T, Murakami K. Cloning and characterization of a gene affecting the methicillin resistance level and the autolysis rate in Staphylococcus aureus. J Bacteriol. 1994;176:4993-5000 pubmed
    ..the transformants of most strains, instances of reduced resistance were observed with concomitant increases of autolysis rate induced by Triton X-100 and were generally more profound in high-resistance strains...
  19. Gustafson J, Berger Bachi B, Strassle A, Wilkinson B. Autolysis of methicillin-resistant and -susceptible Staphylococcus aureus. Antimicrob Agents Chemother. 1992;36:566-72 pubmed
    ..Faster rates of autolysis were noted in two heterogeneous methicillin-resistant transductants than in their methicillin-susceptible ..
  20. Viccini G, Martinelli T, Cognialli R, de Faria R, Carbonero E, Sassaki G, et al. Exopolysaccharide from surface-liquid culture of Clonostachys rosea originates from autolysis of the biomass. Arch Microbiol. 2009;191:369-78 pubmed publisher
    ..We propose that the galactomannan in the culture broth originates from autolysis of the biomass, based not only on the similarity that it has with the galactomannan extracted from the biomass ..
  21. Szilagyi M, Kwon N, Dorogi C, Pocsi I, Yu J, Emri T. The extracellular ?-1,3-endoglucanase EngA is involved in autolysis of Aspergillus nidulans. J Appl Microbiol. 2010;109:1498-508 pubmed publisher
    To elucidate the roles of the ?-1,3-endoglucanase EngA in autolysis of the filamentous fungus Aspergillus nidulans and to identify the common regulatory elements of autolytic hydrolases.
  22. Wang D, Jin Q, Xiang H, Wang W, Guo N, Zhang K, et al. Transcriptional and functional analysis of the effects of magnolol: inhibition of autolysis and biofilms in Staphylococcus aureus. PLoS ONE. 2011;6:e26833 pubmed publisher
    ..Magnolol (MOL) shows inhibitory activity against S. aureus biofilms and Triton X-100-induced autolysis in vitro, although there are no data regarding the molecular mechanisms of MOL action in bacteria.
  23. Margot P, Mauel C, Karamata D. The gene of the N-acetylglucosaminidase, a Bacillus subtilis 168 cell wall hydrolase not involved in vegetative cell autolysis. Mol Microbiol. 1994;12:535-45 pubmed
    ..Cell separation, motility, autolysis, cell wall turnover and growth were not affected in strains devoid of the N-acetylglucosaminidase...
  24. Elce J, Hegadorn C, Arthur J. Autolysis, Ca2+ requirement, and heterodimer stability in m-calpain. J Biol Chem. 1997;272:11268-75 pubmed
    The roles of N-terminal autolysis of the large (80 kDa) and small (28 kDa) subunits in activation of rat m-calpain, in lowering its Ca2+ requirement, and in reducing its stability have been investigated with heterodimeric recombinant ..
  25. Rice K, Peralta R, Bast D, de Azavedo J, McGavin M. Description of staphylococcus serine protease (ssp) operon in Staphylococcus aureus and nonpolar inactivation of sspA-encoded serine protease. Infect Immun. 2001;69:159-69 pubmed publisher
    ..Therefore, we conclude that SspA is required for maturation of SspB and plays a role in controlling autolytic activity but does not by itself exert a significant contribution to the development of tissue abscess infections...
  26. Monleón E, Monzon M, Hortells P, Vargas A, Badiola J. Detection of PrP(sc) in samples presenting a very advanced degree of autolysis (BSE liquid state) by immunocytochemistry. J Histochem Cytochem. 2003;51:15-8 pubmed
    ..encephalopathies (TSEs), has been previously demonstrated on formalin-fixed autolytic tissue, no samples with autolysis as severe as tested here (i.e., liquid state) have previously been tested...
  27. Baki A, Tompa P, Alexa A, Molnar O, Friedrich P. Autolysis parallels activation of mu-calpain. Biochem J. 1996;318 ( Pt 3):897-901 pubmed
    The kinetics of autolysis and activation of mu-calpain were measured with microtubule-associated protein 2 (MAP2) as a very sensitive substrate...
  28. Nishizaki K, Yoshino T, Orita Y, Nomiya S, Masuda Y. TUNEL staining of inner ear structures may reflect autolysis, not apoptosis. Hear Res. 1999;130:131-6 pubmed
    ..TUNEL staining detects not only apoptosis but also late necrosis or autolysis because it detects DNA breaks. Autolysis may occur in inner ear structures during fixation...
  29. Emri T, Molnar Z, Pusztahelyi T, Varecza Z, Pocsi I. The fluG-BrlA pathway contributes to the initialisation of autolysis in submerged Aspergillus nidulans cultures. Mycol Res. 2005;109:757-63 pubmed
    The fluG gene proved to be essential in the initialisation of autolysis in Aspergillus nidulans (teleomorph Emericella nidulans) cultures, while a loss-of-function mutation in only one out of the flbB-E genes had only minor effects on ..
  30. Eakpetch P, Benjakul S, Visessanguan W, Kijroongrojana K. Autolysis of Pacific white shrimp (Litopenaeus vannamei) meat: characterization and the effects of protein additives. J Food Sci. 2008;73:S95-103 pubmed publisher
    ..The autolysis was more pronounced in the acidic pH values, followed by alkaline pH ranges...
  31. Xue T, Zhao L, Sun B. LuxS/AI-2 system is involved in antibiotic susceptibility and autolysis in Staphylococcus aureus NCTC 8325. Int J Antimicrob Agents. 2013;41:85-9 pubmed publisher
    ..aureus. In addition, a luxS mutant exhibited decreased autolysis and upregulated vancomycin resistance-associated VraRS two-component regulatory system...
  32. Daniel K, Anderson J, Zhong Q, Kazi A, Gupta P, Dou Q. Association of mitochondrial calpain activation with increased expression and autolysis of calpain small subunit in an early stage of apoptosis. Int J Mol Med. 2003;12:247-52 pubmed
    ..Our results suggest that increased expression and autolysis of the mitochondrial calpain small subunit are tightly associated with calpain activation in an early stage of ..
  33. Webster M, Francis P, Procter A, Stratmann G, Doshi R, Mann D, et al. Postmortem brains reveal similar but not identical amyloid precursor protein-like immunoreactivity in Alzheimer compared with other dementias. Brain Res. 1994;644:347-51 pubmed
    ..However, the total soluble APPLIR was higher in AD and it was hypothesized that this relates to cholinergic hypoactivity...
  34. Nieto Arribas P, Seseña S, Poveda J, Palop L, Cabezas L. Genotypic and technological characterization of Lactococcus lactis isolates involved in processing of artisanal Manchego cheese. J Appl Microbiol. 2009;107:1505-17 pubmed publisher
    ..Genotypic and technological characterization of wild lactococci isolated from artisanal Manchego cheese during the ripening process for selection of suitable starter cultures...
  35. Gabrijelcic Geiger D, Mentele R, Meisel B, Hinz H, Assfalg Machleidt I, Machleidt W, et al. Human micro-calpain: simple isolation from erythrocytes and characterization of autolysis fragments. Biol Chem. 2001;382:1733-7 pubmed
    ..This novel autolysis product of the small subunit starts with the sequence 60RILG and is further processed to the known 18 kDa ..
  36. Chitnis S, Kameswari D, Prasad K. Induction of autolysis in starved Escherichia coli cells by seminalplasmin, an antimicrobial protein from bovine seminal plasma. FEMS Microbiol Lett. 1993;112:147-50 pubmed
    ..These observations suggest that, unlike many other antibiotics, seminalplasmin can induce autolysis under the conditions of a stringent response.
  37. Ousey K, McIntosh C. Understanding wound bed preparation and wound debridement. Br J Community Nurs. 2010;15:S22, S24, S26, passim pubmed
    ..The method of debridement should be discussed with the patient and family where appropriate and consent to treatment obtained prior to the procedure being undertaken...
  38. Querido D. Temperature-correction of abdominal impedance: improved relationship between impedance and postmortem interval. Forensic Sci Int. 2000;109:39-50 pubmed
    ..interval (PMI) reflects the combined effects, on impedance, of postmortem cooling of the tissues and of autolysis per se...
  39. Sahin Toth M. The pathobiochemistry of hereditary pancreatitis: studies on recombinant human cationic trypsinogen. Pancreatology. 2001;1:461-5 pubmed
    ..This study attempts to identify the biochemical alterations in human cationic trypsinogen and trypsin caused by the hereditary pancreatitis-associated mutations Arg117-->His and Asn21-->Ile...
  40. Peichoto M, Paes Leme A, Pauletti B, Batista I, Mackessy S, Acosta O, et al. Autolysis at the disintegrin domain of patagonfibrase, a metalloproteinase from Philodryas patagoniensis (Patagonia Green Racer; Dipsadidae) venom. Biochim Biophys Acta. 2010;1804:1937-42 pubmed publisher
    ..Herein we demonstrate that patagonfibrase undergoes autolysis at its pH optimum (7.5) and at 37 degrees C, primarily producing a approximately 32...
  41. Murphy R, Vissing K, Latchman H, Lamboley C, McKenna M, Overgaard K, et al. Activation of skeletal muscle calpain-3 by eccentric exercise in humans does not result in its translocation to the nucleus or cytosol. J Appl Physiol (1985). 2011;111:1448-58 pubmed publisher
    ..To test this and help identify how/where calpain-3 acts, we investigated whether calpain-3 autolysis (hence, activation) following eccentric exercise results in translocation from its normal myofibrillar location ..
  42. Karlsson M, Jacobsson B. Intrauterine fetal death associated with Rothia dentocariosa: a case report. Am J Obstet Gynecol. 2007;197:e6-7 pubmed
    ..A healthy primiparous woman was admitted at term with an intrauterine fetal death after an uncomplicated pregnancy. R. dentocariosa was found in the fetal blood culture...
  43. Yang L, Manithody C, Rezaie A. The role of autolysis loop in determining the specificity of coagulation proteases. Braz J Med Biol Res. 2007;40:1055-64 pubmed
    We recently demonstrated that the substitution of the autolysis loop (residues 143 to 154 in the chymotrypsin numbering system) of activated protein C (APC) with the corresponding loop of factor Xa (fXa) renders the APC mutant (APC/fX143-..
  44. Schimmelpfenning D, Mollenhauer S. Use of a clear absorbent acrylic dressing for debridement. J Wound Ostomy Continence Nurs. 2006;33:639-42; discussion 642-3 pubmed
  45. Gräntzdörffer I, Yumlu S, Gioeva Z, von Wasielewski R, Ebert M, Rocken C. Comparison of different tissue sampling methods for protein extraction from formalin-fixed and paraffin-embedded tissue specimens. Exp Mol Pathol. 2010;88:190-6 pubmed publisher
    ..In this study, we explored the possibility to standardize tissue sampling from paraffin blocks and compared the protein extracts with those obtained from fresh frozen material...
  46. Campbell J, Singh A, SWOBODA J, Gilmore M, Wilkinson B, Walker S. An antibiotic that inhibits a late step in wall teichoic acid biosynthesis induces the cell wall stress stimulon in Staphylococcus aureus. Antimicrob Agents Chemother. 2012;56:1810-20 pubmed publisher
    ..We conclude that WTA inhibitors that act at a late stage of the biosynthetic pathway may be useful as antibiotics, and we present evidence that they could be particularly useful in combination with beta-lactams...
  47. Erman A, Veranic P. Time- and temperature-dependent autolysis of urinary bladder epithelium during ex vivo preservation. Protoplasma. 2011;248:541-50 pubmed publisher
    ..These findings provide evidence that room temperature is most appropriate for short ex vivo preservation of urothelial tissue...
  48. Lødemel J, Egge Jacobsen W, Olsen R. Detection of TIMP-2-like protein in Atlantic cod (Gadus morhua) muscle using two-dimensional real-time reverse zymography. Comp Biochem Physiol B Biochem Mol Biol. 2004;139:253-9 pubmed
    ..Finally, de novo sequencing of two peptides derived from the cod muscle inhibitor showed high homology to TIMP-2s both from human and other teleosts...
  49. Yang L, Manithody C, Rezaie A. The functional significance of the autolysis loop in protein C and activated protein C. Thromb Haemost. 2005;94:60-8 pubmed
    The autolysis loop of activated protein C (APC) is five residues longer than the autolysis loop of other vitamin K-dependent coagulation proteases...
  50. Motaouakkil S, Charra B, Hachimi A, Ezzouine H, Guedari H, Nejmi H, et al. [Rhabdomyolysis and paraphenylene-diamine poisoning]. Ann Fr Anesth Reanim. 2006;25:708-13 pubmed
    ..The aim of this study is to describe clinical description, biological findings, outcome and prognostic factors of paraphenylene-diamine poisoning...
  51. Cebollero E, Gonzalez Ramos D, Gonzalez R. Construction of a recombinant autolytic wine yeast strain overexpressing the csc1-1 allele. Biotechnol Prog. 2009;25:1598-604 pubmed publisher
    ..of sparkling wines and wines aged on lees, yeast cells kept in contact with the wine finally die and undergo autolysis, releasing cellular compounds with a positive effect on the wine quality...
  52. Vermaelen M, Sirvent P, Raynaud F, Astier C, Mercier J, Lacampagne A, et al. Differential localization of autolyzed calpains 1 and 2 in slow and fast skeletal muscles in the early phase of atrophy. Am J Physiol Cell Physiol. 2007;292:C1723-31 pubmed
    ..Moreover, the activation of proteolytic pathways appears to differ in slow and fast muscles, and the proteolytic mechanisms involved in fast-type muscle atrophy remain unclear...
  53. Liu Y, Zhou D, Ma D, Liu Y, Li D, Dong X, et al. Changes in collagenous tissue microstructures and distributions of cathepsin L in body wall of autolytic sea cucumber (Stichopus japonicus). Food Chem. 2016;212:341-8 pubmed publisher
    The autolysis of sea cucumber (Stichopus japonicus) was induced by ultraviolet (UV) irradiation, and the changes of microstructures of collagenous tissues and distributions of cathepsin L were investigated using histological and ..