Summary: Occurrence or induction of release of more ova than are normally released at the same time in a given species. The term applies to both animals and humans.

Top Publications

  1. Holzer H, Casper R, Tulandi T. A new era in ovulation induction. Fertil Steril. 2006;85:277-84 pubmed
    To evaluate the efficacy of aromatase inhibitors in ovulation induction, superovulation, and IVF...
  2. Kimura K, Hirako M, Iwata H, Aoki M, Kawaguchi M, Seki M. Successful superovulation of cattle by a single administration of FSH in aluminum hydroxide gel. Theriogenology. 2007;68:633-9 pubmed vitro and in vivo, and whether a single administration of FSH in Al-gel could successfully induce superovulation (SOV) in cattle. Porcine FSH (pFSH; 30 mg) was mixed with 5 mL of Al-gel; 99.98+/-0...
  3. Badawy A, Elnashar A, Totongy M. Clomiphene citrate or aromatase inhibitors for superovulation in women with unexplained infertility undergoing intrauterine insemination: a prospective randomized trial. Fertil Steril. 2009;92:1355-9 pubmed publisher
    To compare clomiphene citrate (CC) and letrozole used for superovulation before intrauterine insemination (IUI) in unexplained infertility. Prospective randomized trial. A university teaching hospital and a private practice setting...
  4. Badawy A, Elnashar A, Totongy M. Clomiphene citrate or aromatase inhibitors combined with gonadotropins for superovulation in women undergoing intrauterine insemination: a prospective randomised trial. J Obstet Gynaecol. 2010;30:617-21 pubmed publisher
    ..This approach will reduce the cost of stimulation protocols without effect on the treatment outcome and letrozole has no advantage over CC in this respect. ..
  5. Humblot P, Holm P, Lonergan P, Wrenzycki C, Lequarre A, Joly C, et al. Effect of stage of follicular growth during superovulation on developmental competence of bovine oocytes. Theriogenology. 2005;63:1149-66 pubmed
    ..However, a precisely defined time for aspirating immature oocytes for subsequent in vitro development seems not to be crucial. ..
  6. Sbracia M, Colabianchi J, Giallonardo A, Giannini P, Piscitelli C, Morgia F, et al. Cetrorelix protocol versus gonadotropin-releasing hormone analog suppression long protocol for superovulation in intracytoplasmic sperm injection patients older than 40. Fertil Steril. 2009;91:1842-7 pubmed publisher
    ..Our study showed that the long protocol performed better in older women than the cetrorelix protocol and that the GnRH antagonist may be detrimental in older women. ..
  7. Wahlberg P, Nylander A, Ahlskog N, Liu K, Ny T. Expression and localization of the serine proteases high-temperature requirement factor A1, serine protease 23, and serine protease 35 in the mouse ovary. Endocrinology. 2008;149:5070-7 pubmed publisher
    ..PRSS35 was also expressed in the forming and regressing CL. These data suggest that HtrA1 and PRSS35 may be involved in ovulation and CL formation and regression, and that PRSS23 may play a role in follicular atresia. ..
  8. Cornejo Cortés M, Sanchez Torres C, Vázquez Chagoyán J, Suárez Gómez H, Garrido Fariña G, Meraz Ríos M. Rat embryo quality and production efficiency are dependent on gonadotrophin dose in superovulatory treatments. Lab Anim. 2006;40:87-95 pubmed
    The present study was performed to optimize a superovulation protocol in rats in order to produce a large number of good-quality embryos suitable to develop rat embryonic stem (rES) cells...
  9. Tríbulo A, Rogan D, Tríbulo H, Tríbulo R, Alasino R, Beltramo D, et al. Superstimulation of ovarian follicular development in beef cattle with a single intramuscular injection of Folltropin-V. Anim Reprod Sci. 2011;129:7-13 pubmed publisher

More Information


  1. Lee W, Song K, Lim K, Lee S, Lee B, Jang G. Influence of factors during superovulation on embryo production in Korean Holstein cattle. J Vet Med Sci. 2012;74:167-74 pubmed
    ..Furthermore, P/F ratio and MUN could be candidate indicators for selection of high-efficiency donor cows. ..
  2. Thangavelu G, Colazo M, Ambrose D, Oba M, Okine E, Dyck M. Diets enriched in unsaturated fatty acids enhance early embryonic development in lactating Holstein cows. Theriogenology. 2007;68:949-57 pubmed
    ..4+/-6.3, 132.3+/-8.3, and 89.3+/-9.6 cells, respectively). Although our hypothesis was only partially supported, embryonic development was enhanced in Holstein cows fed unsaturated fatty acids compared to those fed saturated fatty acids. ..
  3. Mapletoft R, Steward K, Adams G. Recent advances in the superovulation in cattle. Reprod Nutr Dev. 2002;42:601-11 pubmed
    ..Methods can be used for repeated superstimulation of donor animals at 25 to 30 day intervals, without regard to estrus detection or stage of the estrous cycle, and without compromising embryo production. ..
  4. Fauque P, Jouannet P, Lesaffre C, Ripoche M, Dandolo L, Vaiman D, et al. Assisted Reproductive Technology affects developmental kinetics, H19 Imprinting Control Region methylation and H19 gene expression in individual mouse embryos. BMC Dev Biol. 2007;7:116 pubmed
    ..In this study, we evaluated the impact of superovulation, in vitro fertilization (IVF) and embryo culture conditions on proper genomic imprinting and blastocyst ..
  5. Kon H, Tohei A, Hokao R, Shinoda M. Estrous cycle stage-independent treatment of PMSG and hCG can induce superovulation in adult Wistar-Imamichi rats. Exp Anim. 2005;54:185-7 pubmed
    ..These results demonstrate that the PMSG/hCG treatment can induce ovulation at any stage of estrous cycle in WI rats and efficient superovulation at 12 weeks of age.
  6. Yang W, Li S, Tang K, Hua G, Zhang C, Yu J, et al. Polymorphisms in the 5' upstream region of the FSH receptor gene, and their association with superovulation traits in Chinese Holstein cows. Anim Reprod Sci. 2010;119:172-7 pubmed publisher
    To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, variation in the bovine follicle stimulating hormone receptor (FSHR) gene was investigated ..
  7. Popova E, Bader M, Krivokharchenko A. Strain differences in superovulatory response, embryo development and efficiency of transgenic rat production. Transgenic Res. 2005;14:729-38 pubmed
    The differences between rat strains in superovulation response, in vitro and in vivo development of preimplantation embryos and overall transgenic efficiency was studied...
  8. Ishigame H, Medan M, Kawaguchi M, Fukuda A, Watanabe G, Arai K, et al. Induction of superovulation by immunoneutralization of endogenous inhibin in immature rats. J Reprod Dev. 2005;51:559-66 pubmed
    ..Efficiency of superovulation on production of fertilized oocytes was compared between the inhibin antiserum (inhibin-AS) and equine ..
  9. Topipat C, Choktanasiri W, Jultanmas R, Weerakiet S, Wongkularb A, Rojanasakul A. A comparison of the effects of clomiphene citrate and the aromatase inhibitor letrozole on superovulation in Asian women with normal ovulatory cycles. Gynecol Endocrinol. 2008;24:145-50 pubmed publisher
    To compare the effect of the aromatase inhibitor letrozole and clomiphene citrate (CC) on superovulation in women with normal ovulation...
  10. Ishigame H, Medan M, Watanabe G, Shi Z, Kishi H, Arai K, et al. A new alternative method for superovulation using passive immunization against inhibin in adult rats. Biol Reprod. 2004;71:236-43 pubmed
    ..inhibin on ovulation rate and embryo development in vivo and in vitro to establish a new alternative superovulation method in the adult rat...
  11. Tang K, Li S, Yang W, Yu J, Han L, Li X, et al. An MspI polymorphism in the inhibin alpha gene and its associations with superovulation traits in Chinese Holstein cows. Mol Biol Rep. 2011;38:17-21 pubmed publisher
    To identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism, the PCR-RFLP method was applied to detect an A>G transition determining an MspI ..
  12. Yang W, Tang K, Li S, Yang L. Association analysis between variants in bovine progesterone receptor gene and superovulation traits in Chinese Holstein cows. Reprod Domest Anim. 2011;46:1029-34 pubmed publisher
    The objective of this study was to identify a predictor to forecast superovulation response on the basis of associations between superovulation performance and gene polymorphism...
  13. Petit H, Cavalieri F, Santos G, Morgan J, Sharpe P. Quality of embryos produced from dairy cows fed whole flaxseed and the success of embryo transfer. J Dairy Sci. 2008;91:1786-90 pubmed publisher
    ..The experimental diets were fed to 121 recipient heifers from wk 8 before estrus synchronization and superovulation to d 50 of gestation. Dietary fat fed to donor cows had no effect on the number of viable embryos per cow (3...
  14. Ha C, Joo B, Kim S, Joo J, Kim H, Lee K. Estrogen administration during superovulation increases oocyte quality and expressions of vascular endothelial growth factor and nitric oxide synthase in the ovary. J Obstet Gynaecol Res. 2010;36:789-95 pubmed publisher
    This study investigated whether estrogen administration during superovulation enhances oocyte quality using a mice model...
  15. Souter I, Baltagi L, Kuleta D, Meeker J, Petrozza J. Women, weight, and fertility: the effect of body mass index on the outcome of superovulation/intrauterine insemination cycles. Fertil Steril. 2011;95:1042-7 pubmed publisher
  16. Yang W, Tang K, Li S, Chao L, Yang L. Polymorphisms of the bovine luteinizing hormone/choriogonadotropin receptor (LHCGR) gene and its association with superovulation traits. Mol Biol Rep. 2012;39:2481-7 pubmed publisher
    ..transfer technique in cattle is the highly variable between individuals in ovulatory response to FSH-induced superovulation. The objective of this study was to identify a predictor to forecast superovulation response on the basis of ..
  17. Merriman J, Jennings P, McLaughlin E, Jones K. Effect of aging on superovulation efficiency, aneuploidy rates, and sister chromatid cohesion in mice aged up to 15 months. Biol Reprod. 2012;86:49 pubmed publisher
    ..The mean number of eggs ± SEM recovered from mice following superovulation peaked at 22.5 ± 3...
  18. Sato A, Otsu E, Negishi H, Utsunomiya T, Arima T. Aberrant DNA methylation of imprinted loci in superovulated oocytes. Hum Reprod. 2007;22:26-35 pubmed
    ..However, the timing of DNA methylation acquisition in adult oocytogenesis and the effects of superovulation are unknown...
  19. Zhang L, Chai M, Tian X, Wang F, Fu Y, He C, et al. Effects of melatonin on superovulation and transgenic embryo transplantation in small-tailed han sheep (Ovis aries). Neuro Endocrinol Lett. 2013;34:294-301 pubmed
    In this study, the effects of melatonin on superovulation and the transfer of transgenic embryos were investigated in Small-Tailed Han sheep...
  20. Byers S, Payson S, Taft R. Performance of ten inbred mouse strains following assisted reproductive technologies (ARTs). Theriogenology. 2006;65:1716-26 pubmed
    b>Superovulation, in vitro fertilization, embryo cryopreservation, and embryo transfer are assisted reproductive technologies (ARTs) widely used in laboratory mice...
  21. Deb K, Chaturvedi M, Jaiswal Y. Gram-negative bacterial LPS induced poor uterine receptivity and implantation failure in mouse: alterations in IL-1beta expression in the preimplantation embryo and uterine horns. Infect Dis Obstet Gynecol. 2005;13:125-33 pubmed
    ..However, a change in the expression pattern of IL-1beta in the preimplantation embryos underlines the significance of this molecule in LPS induced pregnancy loss or implantation failure in mouse. ..
  22. Forcada F, Abecia J, Cebrian Perez J, Muino Blanco T, Valares J, Palacin I, et al. The effect of melatonin implants during the seasonal anestrus on embryo production after superovulation in aged high-prolificacy Rasa Aragonesa ewes. Theriogenology. 2006;65:356-65 pubmed
    ..administered in March on the ovarian cyclicity, ovulatory response and embryo production after repeated superovulation of selected high-prolificacy Rasa Aragonesa aged ewes...
  23. Claman P, Wilkie V, Collins D. Timing intrauterine insemination either 33 or 39 hours after administration of human chorionic gonadotropin yields the same pregnancy rates as after superovulation therapy. Fertil Steril. 2004;82:13-6 pubmed
    To compare a short and long interval between hCG administration and IUI after superovulation for the treatment of infertility. Prospective, randomized clinical trial. University hospital-based fertility clinic...
  24. Casper R. Letrozole: ovulation or superovulation?. Fertil Steril. 2003;80:1335-7; discussion 1339 pubmed
  25. Market Velker B, Zhang L, Magri L, Bonvissuto A, Mann M. Dual effects of superovulation: loss of maternal and paternal imprinted methylation in a dose-dependent manner. Hum Mol Genet. 2010;19:36-51 pubmed publisher
    b>Superovulation or ovarian stimulation is currently an indispensable assisted reproductive technology (ART) for human subfertility/infertility treatment...
  26. Sommer J, Collins E, Estrada J, Petters R. Synchronization and superovulation of mature cycling gilts for the collection of pronuclear stage embryos. Anim Reprod Sci. 2007;100:402-10 pubmed
    ..A standard dose of each drug was used independent of size or age of the animal. One protocol averaged 38.9 ovulations and 31.1 one-cell embryos recovered per animal. ..
  27. Mitwally M, Casper R. Potential of aromatase inhibitors for ovulation and superovulation induction in infertile women. Drugs. 2006;66:2149-60 pubmed
    ..However, we believe that definitive studies in the form of randomised controlled trials comparing clomifene with AIs are needed. ..
  28. Koppan M, Varnagy A, Reglodi D, Brubel R, Nemeth J, Tamas A, et al. Correlation between oocyte number and follicular fluid concentration of pituitary adenylate cyclase-activating polypeptide (PACAP) in women after superovulation treatment. J Mol Neurosci. 2012;48:617-22 pubmed
    ..was to investigate whether there is a correlation between PACAP concentration in FF and ovarian response to superovulation treatment in infertile women, performed in volunteers (n?=?132; aged between 20 and 35)...
  29. Lee D, Joo B, Suh D, Park J, Choi Y, Lee K. Sodium nitroprusside treatment during the superovulation process improves ovarian response and ovarian expression of vascular endothelial growth factor in aged female mice. Fertil Steril. 2008;89:1514-21 pubmed publisher
    To investigate whether sodium nitroprusside (SNP) treatment during the superovulation process improves ovarian response and oocyte developmental competence in aged female mice. Controlled experimental study. Large urban medical center...
  30. Al Fozan H, Al Khadouri M, Tan S, Tulandi T. A randomized trial of letrozole versus clomiphene citrate in women undergoing superovulation. Fertil Steril. 2004;82:1561-3 pubmed
    ..5 mg) and clomiphene citrate (CC; 100 mg) in women undergoing superovulation and IUI. Prospective randomized trial. University teaching hospital...
  31. Williams R, Hillard J, De Vane G, Yeko T, Kipersztok S, Rhoton Vlasak A, et al. A randomized, multicenter study comparing the efficacy of recombinant FSH vs recombinant FSH with Ganirelix during superovulation/IUI therapy. Am J Obstet Gynecol. 2004;191:648-51; discussion 651-3 pubmed
    ..of a Gonadotropin releasing hormone (GnRH) antagonist, Ganirelix (Antagon), can improve pregnancy rates during superovulation with recombinant follicle-stimulating hormone (rFSH) followed by intrauterine insemination (IUI)...
  32. Healey S, Tan S, Tulandi T, Biljan M. Effects of letrozole on superovulation with gonadotropins in women undergoing intrauterine insemination. Fertil Steril. 2003;80:1325-9 pubmed
    To investigate the effects of letrozole in patients undergoing superovulation with gonadotropins and IUI. Retrospective analysis. Academic teaching hospital...
  33. Zanetti E, Duarte J. Comparison of three protocols for superovulation of brown brocket deer (Mazama gouazoubira). Zoo Biol. 2012;31:642-55 pubmed publisher
    ..and the presence of functional corpora lutea after treatment by three different protocols designed to cause superovulation in brown brocket deer. Six female received an intravaginal device containing 0...
  34. Cuello C, Berthelot F, Martinat Botté F, Guillouet P, Furstoss V, Boisseau C, et al. Transfer of vitrified blastocysts from one or two superovulated Large White Hyperprolific donors to Meishan recipients: reproductive parameters at Day 30 of pregnancy. Theriogenology. 2004;61:843-50 pubmed
    ..05) when transfers were performed with embryos from two donors (19.5% versus 30.5%). These results indicate that pooling embryos from two donors increases the in vivo efficiency after transfer of vitrified/warmed porcine blastocysts. ..
  35. Zech N, Murtinger M, Uher P. Pregnancy after ovarian superovulation by transdermal delivery of follicle-stimulating hormone. Fertil Steril. 2011;95:2784-5 pubmed publisher
    ..We report the first pregnancy after laser microporation and transdermal delivery of FSH for ovarian superovulation as a substitute for SC or IM injections.
  36. Denomme M, Zhang L, Mann M. Embryonic imprinting perturbations do not originate from superovulation-induced defects in DNA methylation acquisition. Fertil Steril. 2011;96:734-738.e2 pubmed publisher
    To investigate whether superovulation disrupts maternal imprint acquisition in oocytes. Animal model. Academic institute. Spontaneously ovulated and superovulated mice...
  37. Choi K, Joo B, Sun S, Park M, Son J, Joo J, et al. Administration of visfatin during superovulation improves developmental competency of oocytes and fertility potential in aged female mice. Fertil Steril. 2012;97:1234-41.e1-3 pubmed publisher
    To examine whether visfatin administration during superovulation improves ovarian response, developmental competence of oocytes, and fertility in aged female mice. Controlled experimental study. University hospital...
  38. Hayakawa H, Hirai T, Takimoto A, Ideta A, Aoyagi Y. Superovulation and embryo transfer in Holstein cattle using sexed sperm. Theriogenology. 2009;71:68-73 pubmed publisher
    ..We concluded that the use of sexed frozen-thawed sperm (> or =90% X-sperm biased and 10 x10(6) total sperm) may be economically viable for commercial MOET programs in Holstein heifers. ..
  39. Burdette J, Kurley S, Kilen S, Mayo K, Woodruff T. Gonadotropin-induced superovulation drives ovarian surface epithelia proliferation in CD1 mice. Endocrinology. 2006;147:2338-45 pubmed
    ..In this study, proliferation of the OSE was quantified in response to superovulation induced by ip injection of pregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) in ..
  40. Fortier A, Lopes F, Darricarrère N, Martel J, Trasler J. Superovulation alters the expression of imprinted genes in the midgestation mouse placenta. Hum Mol Genet. 2008;17:1653-65 pubmed publisher
    ..b>Superovulation has been shown to affect growth and development of the embryo, but an effect on imprinted genes has not been ..
  41. Sotomaru Y, Kamisako T, Hioki K. Estrous stage- and animal age-independent superovulation in the BrlHan:WIST@Jcl(GALAS) rat. Exp Anim. 2005;54:137-41 pubmed
    In most strains of rats, the effects of treatment for the induction of superovulation show major strain differences and are strongly influenced by the stage of the estrous cycle...
  42. Ax R, Armbrust S, Tappan R, Gilbert G, Oyarzo J, Bellin M, et al. Superovulation and embryo recovery from peripubertal Holstein heifers. Anim Reprod Sci. 2005;85:71-80 pubmed
    ..These studies were designed to evaluate feasibility of superovulation and embryo recovery in peripubertal heifers (starting at 7...
  43. Stouder C, Deutsch S, Paoloni Giacobino A. Superovulation in mice alters the methylation pattern of imprinted genes in the sperm of the offspring. Reprod Toxicol. 2009;28:536-41 pubmed publisher
    Some steps of the assisted reproduction techniques, such as superovulation, may interfere with imprinting reprogramming...
  44. Fouda U, Sayed A. Extended letrozole regimen versus clomiphene citrate for superovulation in patients with unexplained infertility undergoing intrauterine insemination: a randomized controlled trial. Reprod Biol Endocrinol. 2011;9:84 pubmed publisher
    ..of extended letrozole regimen with clomiphene citrate in women with unexplained infertility undergoing superovulation and intrauterine insemination (IUI)...
  45. Lozano J, Lonergan P, Boland M, O CALLAGHAN D. Influence of nutrition on the effectiveness of superovulation programmes in ewes: effect on oocyte quality and post-fertilization development. Reproduction. 2003;125:543-53 pubmed
    ..In Expt 1, a lower superovulation response in terms of animals ovulating (P < 0.05), ovulation rate per ewe ovulating (P = 0...
  46. Martin Coello J, Gonzalez R, Crespo C, Gomendio M, Roldan E. Superovulation and in vitro oocyte maturation in three species of mice (Mus musculus, Mus spretus and Mus spicilegus). Theriogenology. 2008;70:1004-13 pubmed publisher
    Mouse oocytes can be obtained via superovulation or using in vitro maturation although several factors, including genetic background, may affect response...
  47. Nargund G, Reid F, Parsons J. Human chorionic gonadotropin-to-oocyte collection interval in a superovulation IVF program. A prospective study. J Assist Reprod Genet. 2001;18:87-90 pubmed
    ..None of the 533 women studied had ovulated before oocyte collection. The results do not suggest a trend toward increased ovulation more than 36 hr after hCG administration. ..
  48. Guzick D, Sullivan M, Adamson G, Cedars M, Falk R, Peterson E, et al. Efficacy of treatment for unexplained infertility. Fertil Steril. 1998;70:207-13 pubmed
    ..Clomiphene citrate + IUI is a cost-effective treatment for unexplained infertility. If this treatment fails, hMG + IUI and assisted reproduction are efficacious therapeutic options. ..
  49. Van Soom A, Boerjan M, Bols P, Vanroose G, Lein A, Coryn M, et al. Timing of compaction and inner cell allocation in bovine embryos produced in vivo after superovulation. Biol Reprod. 1997;57:1041-9 pubmed
    ..It can be concluded that the slow in vivo transition from the morula to the blastocyst stage allows sufficient time for allocation of inner cells to the ICM of the embryo. ..
  50. Bodis J, Bognar Z, Hartmann G, Torok A, Csaba I. Measurement of noradrenaline, dopamine and serotonin contents in follicular fluid of human graafian follicles after superovulation treatment. Gynecol Obstet Invest. 1992;33:165-7 pubmed
    ..The patients were given human menopausal gonadotropin (HMG), as a superovulation treatment, and 7,500 IU human chorionic gonadotropin (HCG) to induce ovulation 34-36 h prior to the follicular ..
  51. Albano C, Grimbizis G, Smitz J, Riethmüller Winzen H, Reissmann T, Van Steirteghem A, et al. The luteal phase of nonsupplemented cycles after ovarian superovulation with human menopausal gonadotropin and the gonadotropin-releasing hormone antagonist Cetrorelix. Fertil Steril. 1998;70:357-9 pubmed
    ..However, a progressive increase in LH was observed after day 7, reaching normal values. Corpus luteum function seems to be impaired in cycles that are stimulated with hMG and the GnRH antagonist Cetrorelix. ..
  52. Macrow P, Li T, Seif M, Buckley C, Elstein M. Endometrial structure after superovulation: a prospective controlled study. Fertil Steril. 1994;61:696-9 pubmed
    To demonstrate the effect of superovulation using a GnRH agonist (GnRH-a) and hMG and hCG on endometrial structure. Prospective, case-controlled study. Tertiary referral assisted reproduction unit in an academic department...
  53. Mills M, Eddowes H, Cahill D, Fahy U, Abuzeid M, McDermott A, et al. A prospective controlled study of in-vitro fertilization, gamete intra-fallopian transfer and intrauterine insemination combined with superovulation. Hum Reprod. 1992;7:490-4 pubmed
    ..fertilization (IVF), gamete intra-Fallopian transfer (GIFT) and intrauterine insemination (IUI) combined with superovulation in the treatment of infertility were compared in 151 couples undergoing a single cycle of treatment...