congo red

Summary

Summary: An acid dye used in testing for hydrochloric acid in gastric contents. It is also used histologically to test for AMYLOIDOSIS.

Top Publications

  1. Maezawa I, Hong H, Liu R, Wu C, Cheng R, Kung M, et al. Congo red and thioflavin-T analogs detect Abeta oligomers. J Neurochem. 2008;104:457-68 pubmed
    ..We found that some well established amyloid ligands, analogs of Congo red and thioflavin-T (ThT), bind AbetaO with high affinity and detect AbetaO in vitro and in vivo...
  2. Zhao J, Liu H, Elumalai P, Chen W, Men L, Liu K. Molecular modeling to investigate the binding of Congo red toward GNNQQNY protofibril and in silico virtual screening for the identification of new aggregation inhibitors. J Mol Model. 2013;19:151-62 pubmed publisher
    ..mechanics Poisson-Boltzmann surface area (MM-PBSA) analyses were integrated to investigate the binding between Congo red (CR) and the GNNQQNY protofibril from yeast prion protein Sup35 and to further evaluate their binding ..
  3. Eisert R, Felau L, Brown L. Methods for enhancing the accuracy and reproducibility of Congo red and thioflavin T assays. Anal Biochem. 2006;353:144-6 pubmed
  4. Sereikaite J, Bumelis V. Congo red interaction with alpha-proteins. Acta Biochim Pol. 2006;53:87-92 pubmed
    The ability of Congo red to form complexes with alpha-proteins, human growth hormone and human interferon-alpha2b, was found by absorption difference spectroscopy...
  5. Lendel C, Bertoncini C, Cremades N, Waudby C, Vendruscolo M, Dobson C, et al. On the mechanism of nonspecific inhibitors of protein aggregation: dissecting the interactions of alpha-synuclein with Congo red and lacmoid. Biochemistry. 2009;48:8322-34 pubmed publisher
    ..a protein whose aggregation is closely connected with Parkinson's disease, and two small molecules, Congo red and Lacmoid, which inhibit its fibrillization...
  6. Reddy M, Sivaramakrishna L, Reddy A. The use of an agricultural waste material, Jujuba seeds for the removal of anionic dye (Congo red) from aqueous medium. J Hazard Mater. 2012;203-204:118-27 pubmed publisher
    ..maruritiana), abundantly available in and around the Nallamalla forest in Andhra Pradesh, for the anionic dye (Congo red, CR) adsorption from aqueous solution, has been investigated as low cost and eco-friendly adsorbent...
  7. Michels J, Büntzow M. Assessment of Congo red as a fluorescence marker for the exoskeleton of small crustaceans and the cuticle of polychaetes. J Microsc. 2010;238:95-101 pubmed publisher
    In this study, the potential of the common dye Congo red as a fluorescence marker for chitin in the exoskeleton of small crustaceans and collagen in the polychaete cuticle was tested...
  8. Hu Z, Chen H, Ji F, Yuan S. Removal of Congo Red from aqueous solution by cattail root. J Hazard Mater. 2010;173:292-7 pubmed publisher
    In this study, cattail root was used to remove Congo Red (CR) from aqueous solution...
  9. Stopa B, Rybarska J, Drozd A, Konieczny L, Krol M, Lisowski M, et al. Albumin binds self-assembling dyes as specific polymolecular ligands. Int J Biol Macromol. 2006;40:1-8 pubmed
    Self-assembling dyes with a structure related to Congo red (e.g. Evans blue) form polymolecular complexes with albumin. The dyes, which are lacking a self-assembling property (Trypan blue, ANS) bind as single molecules...

More Information

Publications62

  1. Yang Y, Wang G, Wang B, Li Z, Jia X, Zhou Q, et al. Biosorption of Acid Black 172 and Congo Red from aqueous solution by nonviable Penicillium YW 01: kinetic study, equilibrium isotherm and artificial neural network modeling. Bioresour Technol. 2011;102:828-34 pubmed publisher
    ..performance of nonviable Penicillium YW 01 biomass for removal of Acid Black 172 metal-complex dye (AB) and Congo Red (CR) in solutions. Maximum biosorption capacities of 225.38 and 411...
  2. Juárez J, Taboada P, Mosquera V. Existence of different structural intermediates on the fibrillation pathway of human serum albumin. Biophys J. 2009;96:2353-70 pubmed publisher
    ..intermediates on the fibrillation pathway were determined by means of thioflavin T (ThT) fluorescence and Congo Red absorbance; far- and near-ultraviolet circular dichroism; tryptophan fluorescence; Fourier transform infrared ..
  3. Antoniani D, Bocci P, Maciag A, Raffaelli N, Landini P. Monitoring of diguanylate cyclase activity and of cyclic-di-GMP biosynthesis by whole-cell assays suitable for high-throughput screening of biofilm inhibitors. Appl Microbiol Biotechnol. 2010;85:1095-104 pubmed publisher
    ..of AdrA-dependent cellulose production as red colony phenotype on solid medium supplemented with the dye Congo red (CR). Presence of DGC inhibitors blocking AdrA activity would result in a white phenotype on CR medium...
  4. Pratim Bose P, Chatterjee U, Xie L, Johansson J, Göthelid E, Arvidsson P. Effects of Congo red on a?(1-40) fibril formation process and morphology. ACS Chem Neurosci. 2010;1:315-24 pubmed publisher
    ..Hence, inhibition of A? aggregation is actively explored as an option to prevent or treat AD. Congo red (CR) has been widely used as a model antiamyloid agent to prevent A? aggregation...
  5. Ramsook C, Tan C, Garcia M, Fung R, Soybelman G, Henry R, et al. Yeast cell adhesion molecules have functional amyloid-forming sequences. Eukaryot Cell. 2010;9:393-404 pubmed publisher
    ..cerevisiae Flo1p and C. albicans Eap1p rapidly formed insoluble amyloids, as determined by Congo red absorbance, thioflavin T fluorescence, and fiber morphology...
  6. Olukanni O, Osuntoki A, Awotula A, Kalyani D, Gbenle G, Govindwar S. Decolorization of dyehouse effluent and biodegradation of Congo red by Bacillus thuringiensis RUN1. J Microbiol Biotechnol. 2013;23:843-9 pubmed
    ..infrared spectroscopy and gas chromatography-mass spectrometer analysis of the ethyl acetate extract of Congo red dye and its metabolites showed that the bacterium could degrade it by the asymmetric cleavage of the azo bonds ..
  7. Zhu H, Jiang R, Xiao L, Chang Y, Guan Y, Li X, et al. Photocatalytic decolorization and degradation of Congo Red on innovative crosslinked chitosan/nano-CdS composite catalyst under visible light irradiation. J Hazard Mater. 2009;169:933-40 pubmed publisher
    ..An azo dye, Congo Red (CR), was used as model pollutant to study its photocatalytic activity under visible light irradiation...
  8. Lendel C, Bolognesi B, Wahlström A, Dobson C, Gräslund A. Detergent-like interaction of Congo red with the amyloid beta peptide. Biochemistry. 2010;49:1358-60 pubmed publisher
    ..b>Congo red (CR) affects protein aggregation...
  9. Ram A, Klis F. Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red. Nat Protoc. 2006;1:2253-6 pubmed
    ..that cell wall mutants are generally more sensitive to two related anionic dyes, Calcofluor white (CFW) and Congo red (CR), both of which interfere with the construction and stress response of the cell wall...
  10. Wang L, Wang A. Adsorption characteristics of Congo Red onto the chitosan/montmorillonite nanocomposite. J Hazard Mater. 2007;147:979-85 pubmed
    ..of CTS and MMT, initial pH value of the dye solution and temperature on adsorption capacities of samples for Congo Red (CR) dye have been investigated...
  11. Piekarska B, Drozd A, Konieczny L, Krol M, Jurkowski W, Roterman I, et al. The indirect generation of long-distance structural changes in antibodies upon their binding to antigen. Chem Biol Drug Des. 2006;68:276-83 pubmed
    ..the site of packing of the N-terminal chain fragment allows penetration and binding of the supramolecular dye Congo red that hence becomes an indicator of the initiated relaxation process and is also the prospective ligand in ..
  12. Dawood S, Sen T. Removal of anionic dye Congo red from aqueous solution by raw pine and acid-treated pine cone powder as adsorbent: equilibrium, thermodynamic, kinetics, mechanism and process design. Water Res. 2012;46:1933-46 pubmed publisher
    ..Batch adsorption experiments were performed to remove anionic dye Congo red from aqueous solution...
  13. Spólnik P, Stopa B, Piekarska B, Jagusiak A, Konieczny L, Rybarska J, et al. The use of rigid, fibrillar Congo red nanostructures for scaffolding protein assemblies and inducing the formation of amyloid-like arrangement of molecules. Chem Biol Drug Des. 2007;70:491-501 pubmed
    ..organization of protein aggregates was obtained using for their formation the rigid fibrillar nanostructures of Congo red as the scaffolding...
  14. Romero D, Aguilar C, Losick R, Kolter R. Amyloid fibers provide structural integrity to Bacillus subtilis biofilms. Proc Natl Acad Sci U S A. 2010;107:2230-4 pubmed publisher
    ..We propose that TasA forms amyloid fibers that bind cells together in the biofilm. ..
  15. Wu C, Wang Z, Lei H, Zhang W, Duan Y. Dual binding modes of Congo red to amyloid protofibril surface observed in molecular dynamics simulations. J Am Chem Soc. 2007;129:1225-32 pubmed
    b>Congo red has been used to identify amyloid fibrils in tissues for more than 80 years and is also a weak inhibitor to both amyloid-beta fibril formation and toxicity...
  16. Pardini G, De Groot P, Coste A, Karababa M, Klis F, de Koster C, et al. The CRH family coding for cell wall glycosylphosphatidylinositol proteins with a predicted transglycosidase domain affects cell wall organization and virulence of Candida albicans. J Biol Chem. 2006;281:40399-411 pubmed
    ..genes of this family resulted in additive sensitivity to compounds interfering with normal cell wall formation (Congo red, calcofluor white, SDS, and high Ca(2+) concentrations), suggesting that these genes contribute to cell wall ..
  17. Iram A, Naeem A. Trifluoroethanol and acetonitrile induced formation of the molten globule states and aggregates of cellulase. Int J Biol Macromol. 2012;50:932-8 pubmed
    ..in intrinsic and ANS fluorescence, loss in tertiary structure and enzyme activity, increase non-native ?-sheet structure as evident from far-UV CD and FTIR spectra, increase in Thioflavin T fluorescence and shift in Congo red assay.
  18. Smid L, Vovko T, Popovic M, Petric A, Kepe V, Barrio J, et al. The 2,6-disubstituted naphthalene derivative FDDNP labeling reliably predicts Congo red birefringence of protein deposits in brain sections of selected human neurodegenerative diseases. Brain Pathol. 2006;16:124-30 pubmed
    ..Our findings may be of significant value for the detection of neuropathological aggregates with [F-18]FDDNP in some of these disorders in the living brain of human subjects. ..
  19. McLaughlin R, De Stigter J, Sikkink L, Baden E, Ramirez Alvarado M. The effects of sodium sulfate, glycosaminoglycans, and Congo red on the structure, stability, and amyloid formation of an immunoglobulin light-chain protein. Protein Sci. 2006;15:1710-22 pubmed
    ..Finally, the histological dye Congo red, known to bind to the cross beta-sheet structure of amyloid fibrils, inhibits AL-09 amyloid fibril formation in ..
  20. de Jong W, Wosten H, Dijkhuizen L, Claessen D. Attachment of Streptomyces coelicolor is mediated by amyloidal fimbriae that are anchored to the cell surface via cellulose. Mol Microbiol. 2009;73:1128-40 pubmed publisher
    ..Mass spectrometry, electron microscopy and Congo red treatment showed that these fimbriae are composed of bundled amyloid fibrils of chaplins...
  21. Shamrock V, Duval J, Lindsey G, Gaboriaud F. The role of the heat shock protein Hsp12p in the dynamic response of Saccharomyces cerevisiae to the addition of Congo red. FEMS Yeast Res. 2009;9:391-9 pubmed publisher
    ..a wild-type (WT) strain and a strain overexpressing (OE) Hsp12p, in the presence and absence of hydrophobic Congo red compound...
  22. Silva R, Carmona Ribeiro A, Petri D. Enzymatic activity of cholesterol oxidase immobilized onto polymer nanoparticles mediated by Congo red. Colloids Surf B Biointerfaces. 2013;110:347-55 pubmed publisher
    ..The adsorption of Congo red (CR) onto PS/PEG particles was evidenced by the decrease of ? potential values and increase in the particles ..
  23. Howie A, Brewer D, Howell D, Jones A. Physical basis of colors seen in Congo red-stained amyloid in polarized light. Lab Invest. 2008;88:232-42 pubmed publisher
    Amyloid stained by Congo red is traditionally said to show apple-green birefringence in polarized light, although in practice various colors may be seen between accurately crossed polarizing filters, called polarizer and analyzer...
  24. Ingham C, Ben Jacob E. Swarming and complex pattern formation in Paenibacillus vortex studied by imaging and tracking cells. BMC Microbiol. 2008;8:36 pubmed publisher
    ..Inhibitors of swarming (p-Nitrophenylglycerol and Congo Red) were identified...
  25. Telke A, Joshi S, Jadhav S, Tamboli D, Govindwar S. Decolorization and detoxification of Congo red and textile industry effluent by an isolated bacterium Pseudomonas sp. SU-EBT. Biodegradation. 2010;21:283-96 pubmed publisher
    ..SU-EBT. This strain showed 97 and 90% decolorization of a recalcitrant dye, Congo red (100 mg l(-1)) and textile industry effluent with 50% reduction in COD within 12 and 60 h, respectively...
  26. Hiller E, Heine S, Brunner H, Rupp S. Candida albicans Sun41p, a putative glycosidase, is involved in morphogenesis, cell wall biogenesis, and biofilm formation. Eukaryot Cell. 2007;6:2056-65 pubmed
    ..In addition, the SUN41 mutant showed a higher sensitivity towards the cell wall-disturbing agent Congo red, whereas no difference was observed in the presence of calcofluor white...
  27. Feng B, Toyama B, Wille H, Colby D, Collins S, May B, et al. Small-molecule aggregates inhibit amyloid polymerization. Nat Chem Biol. 2008;4:197-9 pubmed publisher
    ..They also blocked infection of yeast cells with Sup35 prions, which suggests that colloidal inhibition may be relevant in more biological milieus. ..
  28. Suzuki T, Campbell J, Kim Y, SWOBODA J, Mylonakis E, Walker S, et al. Wall teichoic acid protects Staphylococcus aureus from inhibition by Congo red and other dyes. J Antimicrob Chemother. 2012;67:2143-51 pubmed publisher
    ..of wall teichoic acid expression resulted in an ?1000-fold increase in susceptibility to azo dyes such as Congo red, reducing its MIC from >1024 to <4 mg/L...
  29. Zako T, Sakono M, Hashimoto N, Ihara M, Maeda M. Bovine insulin filaments induced by reducing disulfide bonds show a different morphology, secondary structure, and cell toxicity from intact insulin amyloid fibrils. Biophys J. 2009;96:3331-40 pubmed publisher
    ..The remarkably low toxicity of the filamentous structure should shed new light on possible pharmacological approaches to the various diseases caused by amyloid fibrils. ..
  30. Krol M, Roterman I, Drozd A, Konieczny L, Piekarska B, Rybarska J, et al. The increased flexibility of CDR loops generated in antibodies by Congo red complexation favors antigen binding. J Biomol Struct Dyn. 2006;23:407-16 pubmed
    The dye Congo red and related self-assembling compounds were found to stabilize immune complexes by binding to antibodies currently engaged in complexation to antigen...
  31. Hare J, McDonough K. High-frequency RecA-dependent and -independent mechanisms of Congo red binding mutations in Yersinia pestis. J Bacteriol. 1999;181:4896-904 pubmed
    Yersinia pestis, which causes bubonic and pneumonic plague, forms pigmented red colonies on Congo red (CR) dye agar...
  32. Piekarska B, Konieczny L, Rybarska J, Stopa B, Spolnik P, Roterman I, et al. Intramolecular signaling in immunoglobulins -- new evidence emerging from the use of supramolecular protein ligands. J Physiol Pharmacol. 2004;55:487-501 pubmed
    ..Here we present new evidence supporting the notion of intramolecular signaling, based on the use of supramolecular dyes that bind to signal-derived specific sites in immunoglobulins. ..
  33. Inouye H, Nguyen J, Fraser P, Shinchuk L, Packard A, Kirschner D. Histidine residues underlie Congo red binding to A beta analogs. Amyloid. 2000;7:179-88 pubmed
    The binding mechanism of Congo red (CR) to Alzheimer's disease (AD) amyloid fibrils (A beta) in terms of binding affinity and number of sites was quantitated from absorption spectroscopy (at 200-700 nm) by measuring the concentration of ..
  34. Stopa B, Piekarska B, Konieczny L, Rybarska J, Spólnik P, Zemanek G, et al. The structure and protein binding of amyloid-specific dye reagents. Acta Biochim Pol. 2003;50:1213-27 pubmed
    The self-assembling tendency and protein complexation capability of dyes related to Congo red and also some dyes of different structure were compared to explain the mechanism of Congo red binding and the reason for its specific affinity ..
  35. Kim Y, Randolph T, Manning M, Stevens F, Carpenter J. Congo red populates partially unfolded states of an amyloidogenic protein to enhance aggregation and amyloid fibril formation. J Biol Chem. 2003;278:10842-50 pubmed
    b>Congo red (CR) has been reported to inhibit or enhance amyloid fibril formation by several proteins...
  36. Piekarska B, Konieczny L, Rybarska J, Stopa B, Zemanek G, Szneler E, et al. Heat-induced formation of a specific binding site for self-assembled Congo Red in the V domain of immunoglobulin L chain lambda. Biopolymers. 2001;59:446-56 pubmed
    Moderate heating (40-50 degrees C) of immunoglobulins makes them accessible for binding with Congo Red and some related highly associated dyes...
  37. Rybarska J, Piekarska B, Stopa B, Zemanek G, Konieczny L, Nowak M, et al. Evidence that supramolecular Congo red is the sole ligation form of this dye for L chain lambda derived amyloid proteins. Folia Histochem Cytobiol. 2001;39:307-14 pubmed
    The mechanism of Congo red binding to amyloid protein was studied in order to establish which of two structural dye versions present in water solutions--unimolecular and supramolecular--represent its actual ligation form...
  38. Ashburn T, Han H, McGuinness B, Lansbury P. Amyloid probes based on Congo Red distinguish between fibrils comprising different peptides. Chem Biol. 1996;3:351-8 pubmed
    ..in Alzheimer's disease (brain) and type II diabetes (pancreas), were first visualized by staining with the dye Congo Red (CR)...
  39. Cangelosi G, Palermo C, Laurent J, Hamlin A, Brabant W. Colony morphotypes on Congo red agar segregate along species and drug susceptibility lines in the Mycobacterium avium-intracellulare complex. Microbiology. 1999;145 ( Pt 6):1317-24 pubmed
    ..intracellulare) and intraspecific (within individual M. avium isolates). Clinical isolates of M. avium grown on Congo red (CR) plates formed red, pink or mixed (red and white) opaque colonies, while M...
  40. Linke R. Highly sensitive diagnosis of amyloid and various amyloid syndromes using Congo red fluorescence. Virchows Arch. 2000;436:439-48 pubmed
    ..different methods that can be applied to the diagnosis of amyloid in tissue sections have been compared: the Congo red staining method (CR), the combination of CR and immunocytochemistry (CRIC) and Congo red fluorescence (CRF)...
  41. Roterman I, Krul M, Nowak M, Konieczny L, Rybarska J, Stopa B, et al. Why Congo red binding is specific for amyloid proteins - model studies and a computer analysis approach. Med Sci Monit. 2001;7:771-84 pubmed
    The complexing of Congo red in two different ligand forms - unimolecular and supramolecular (seven molecules in a micelle) - with eight deca-peptides organized in a b-sheet was tested by computational analysis to identify its dye-binding ..
  42. Chiti F, Bucciantini M, Capanni C, Taddei N, Dobson C, Stefani M. Solution conditions can promote formation of either amyloid protofilaments or mature fibrils from the HypF N-terminal domain. Protein Sci. 2001;10:2541-7 pubmed
    ..Therefore, formation and isolation of individual protofilaments appears facilitated under conditions that favor the destabilization of hydrophobic interactions, such as in the presence of trifluoroethanol. ..
  43. Bao Y, Sarkar S, Uyama E, Rubinsztein D. Congo red, doxycycline, and HSP70 overexpression reduce aggregate formation and cell death in cell models of oculopharyngeal muscular dystrophy. J Med Genet. 2004;41:47-51 pubmed
  44. Krol M, Borowski T, Roterman I, Piekarska B, Stopa B, Rybarska J, et al. Force-field parametrization and molecular dynamics simulations of Congo red. J Comput Aided Mol Des. 2004;18:41-53 pubmed
    b>Congo red, a diazo dye widely used in medical diagnosis, is known to form supramolecular systems in solution. Such a supramolecular system may interact with various proteins...
  45. Fowler D, Koulov A, Alory Jost C, Marks M, Balch W, Kelly J. Functional amyloid formation within mammalian tissue. PLoS Biol. 2006;4:e6 pubmed
  46. Arciola C, Campoccia D, Gamberini S, Cervellati M, Donati E, Montanaro L. Detection of slime production by means of an optimised Congo red agar plate test based on a colourimetric scale in Staphylococcus epidermidis clinical isolates genotyped for ica locus. Biomaterials. 2002;23:4233-9 pubmed
    ..icaD genes responsible for slime synthesis by a PCR method and for the in vitro slime production ability by the Congo red agar (CRA) plate test...
  47. Khurana R, Uversky V, Nielsen L, Fink A. Is Congo red an amyloid-specific dye?. J Biol Chem. 2001;276:22715-21 pubmed
    b>Congo red (CR) binding, monitored by characteristic yellow-green birefringence under crossed polarization has been used as a diagnostic test for the presence of amyloid in tissue sections for several decades...
  48. Damaschun G, Damaschun H, Fabian H, Gast K, Krober R, Wieske M, et al. Conversion of yeast phosphoglycerate kinase into amyloid-like structure. Proteins. 2000;39:204-11 pubmed
    ..structure which gives rise to typical infrared spectra, X-ray diffraction and yellow-green birefringence after Congo red staining...
  49. Bates S, MacCallum D, Bertram G, Munro C, Hughes H, Buurman E, et al. Candida albicans Pmr1p, a secretory pathway P-type Ca2+/Mn2+-ATPase, is required for glycosylation and virulence. J Biol Chem. 2005;280:23408-15 pubmed publisher
    ..defect, the null mutant had a weakened cell wall, exemplified by hypersensitivity to Calcofluor white, Congo red, and hygromycin B and constitutive activation of the cell integrity pathway...
  50. Fukui T, Shiraki K, Hamada D, Hara K, Miyata T, Fujiwara S, et al. Thermostable direct hemolysin of Vibrio parahaemolyticus is a bacterial reversible amyloid toxin. Biochemistry. 2005;44:9825-32 pubmed
    ..b>Congo red was found to inhibit the hemolytic activity of TDH in a dose-dependent manner...
  51. Mattson M, Begley J, Mark R, Furukawa K. Abeta25-35 induces rapid lysis of red blood cells: contrast with Abeta1-42 and examination of underlying mechanisms. Brain Res. 1997;771:147-53 pubmed
    ..The damaging effects of both A beta25-35 and A beta1-42 towards RBCs were completely prevented by Congo red indicating a requirement for peptide fibril formation...
  52. Bhattacharyya K, Sharma A. Azadirachta indica leaf powder as an effective biosorbent for dyes: a case study with aqueous Congo Red solutions. J Environ Manage. 2004;71:217-29 pubmed
    ..known as the Neem tree) in the form of a powder were investigated as a biosorbent of dyes taking aqueous Congo Red solution as a model system...
  53. Crowther D, Kinghorn K, Miranda E, Page R, Curry J, Duthie F, et al. Intraneuronal Abeta, non-amyloid aggregates and neurodegeneration in a Drosophila model of Alzheimer's disease. Neuroscience. 2005;132:123-35 pubmed
    ..The fly phenotype is rescued by treatment with Congo Red that reduces Abeta aggregation in vitro...