Summary: Steroids with a hydroxyl group at C-3 and most of the skeleton of cholestane. Additional carbon atoms may be present in the side chain. (IUPAC Steroid Nomenclature, 1987)

Top Publications

  1. Nguyen H, Chau V, Tran T, Phan V, Hoang T, Nguyen T, et al. C29 sterols with a cyclopropane ring at C-25 and 26 from the Vietnamese marine sponge Ianthella sp. and their anticancer properties. Bioorg Med Chem Lett. 2009;19:4584-8 pubmed publisher
    Two new C(29) sterols with a cyclopropane ring at C-25 and C-26, petrosterol-3,6-dione (1) and 5alpha,6alpha-epoxy-petrosterol (2), along with petrosterol (3), were isolated from the Vietnamese marine sponge Ianthella sp...
  2. Jo Y, Lee P, Sguigna P, DeBose Boyd R. Sterol-induced degradation of HMG CoA reductase depends on interplay of two Insigs and two ubiquitin ligases, gp78 and Trc8. Proc Natl Acad Sci U S A. 2011;108:20503-8 pubmed publisher
    Accumulation of sterols in membranes of the endoplasmic reticulum (ER) leads to the accelerated ubiquitination and proteasomal degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a rate-limiting enzyme in synthesis of ..
  3. Kim S, Ta Q. Potential beneficial effects of marine algal sterols on human health. Adv Food Nutr Res. 2011;64:191-8 pubmed publisher
    ..b>Sterols are important structural component of cell membranes...
  4. Li R, Shao C, Qi X, Li X, Li J, Sun L, et al. Polyoxygenated sterols from the South China Sea soft coral Sinularia sp. Mar Drugs. 2012;10:1422-32 pubmed publisher
    ..collected from the South China Sea led to the isolation of three new polyoxygenated sterols, (3S,23R,24S)-ergost-5-ene-3?,23?,25-triol (1), (24S)-ergostane-6-acetate-3?,5?,6?,25-tetraol (2), (24S)-..
  5. Huang H, Liaw C, Yang H, Hseu Y, Kuo H, Tsai Y, et al. Lanostane triterpenoids and sterols from Antrodia camphorata. Phytochemistry. 2012;84:177-83 pubmed publisher
    ..3,7,11,12,15,23-hexaoxo-5?-lanost-8-en-26-oate, and ethyl 3,7,11,12,15,23-hexaoxo-5?-lanost-8-en-26-oate, two sterols, (14?,22E)-14-hydroxyergosta-7,22-diene-3,6-dione and a steroid named as camphosterol A were isolated from a ..
  6. Beste L, Nahar N, Dalman K, Fujioka S, Jonsson L, Dutta P, et al. Synthesis of hydroxylated sterols in transgenic Arabidopsis plants alters growth and steroid metabolism. Plant Physiol. 2011;157:426-40 pubmed publisher
    To explore mechanisms in plant sterol homeostasis, we have here increased the turnover of sterols in Arabidopsis (Arabidopsis thaliana) and potato (Solanum tuberosum) plants by overexpressing four mouse cDNA encoding cholesterol ..
  7. Shin K, Chin J, Hahn D, Lee J, Hwang H, Won D, et al. Sterols from a soft coral, Dendronephthya gigantea as farnesoid X-activated receptor antagonists. Steroids. 2012;77:355-9 pubmed publisher
    ..The steroids 1 and 2 showed notable inhibitory activity against farnesoid X-activated receptor (FXR) with IC(50)'s 14 and 15?M. ..
  8. Ivashina T, Nikolayeva V, Dovbnya D, Donova M. Cholesterol oxidase ChoD is not a critical enzyme accounting for oxidation of sterols to 3-keto-4-ene steroids in fast-growing Mycobacterium sp. VKM Ac-1815D. J Steroid Biochem Mol Biol. 2012;129:47-53 pubmed publisher
    Fast-growing strain of Mycobacterium sp. VKM Ac-1815D is capable of effective oxidizing of sterols (phytosterol, cholesterol, ergosterol) to androstenedione and other valuable 3-oxo-steroids...
  9. McDonald J, Smith D, Stiles A, Russell D. A comprehensive method for extraction and quantitative analysis of sterols and secosteroids from human plasma. J Lipid Res. 2012;53:1399-409 pubmed publisher
    We describe the development of a method for the extraction and analysis of 62 sterols, oxysterols, and secosteroids from human plasma using a combination of HPLC-MS and GC-MS. Deuterated standards are added to 200 ?l of human plasma...

Scientific Experts

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  1. Miettinen T, Gylling H, Nissinen M. The role of serum non-cholesterol sterols as surrogate markers of absolute cholesterol synthesis and absorption. Nutr Metab Cardiovasc Dis. 2011;21:765-9 pubmed publisher
    ..methods of the measurements, new approaches were developed including the analysis of serum non-cholesterol sterols. In current lipidologic papers and even in intervention studies, serum non-cholesterol sterols are frequently ..
  2. Simmons L, Kaufmann K, Garcia R, Schwär G, Huch V, Muller R. Bendigoles D-F, bioactive sterols from the marine sponge-derived Actinomadura sp. SBMs009. Bioorg Med Chem. 2011;19:6570-5 pubmed publisher
    ..Here we report the structure and bioactivity of the bendigoles D-F (1-3), 3-keto sterols isolated from the new marine sponge derived bacterium, Actinomadura sp. SBMs009...
  3. Meljon A, Theofilopoulos S, Shackleton C, Watson G, Javitt N, Knölker H, et al. Analysis of bioactive oxysterols in newborn mouse brain by LC/MS. J Lipid Res. 2012;53:2469-83 pubmed publisher
    ..The desmosterol metabolites (24Z),26-, (24E),26-, and 7?-hydroxydesmosterol were identified in brain for the first time. ..
  4. Fiorucci S, Distrutti E, Bifulco G, D Auria M, Zampella A. Marine sponge steroids as nuclear receptor ligands. Trends Pharmacol Sci. 2012;33:591-601 pubmed publisher
    ..These findings pave the way to the development of novel FXR and PXR agonists and antagonists to target human diseases...
  5. Cheng Z, Xiao H, Fan C, Lu Y, Zhang G, Yin S. Bioactive polyhydroxylated sterols from the marine sponge Haliclona crassiloba. Steroids. 2013;78:1353-8 pubmed publisher
    Four new polyhydroxylated sterols, named halicrasterols A-D (1-4), together with six known analogs (5-10) were isolated from the marine sponge Haliclona crassiloba...
  6. Solà R, Valls R, Godas G, Perez Busquets G, Ribalta J, Girona J, et al. Cocoa, hazelnuts, sterols and soluble fiber cream reduces lipids and inflammation biomarkers in hypertensive patients: a randomized controlled trial. PLoS ONE. 2012;7:e31103 pubmed publisher
    ..Thus, the soluble fiber effects amplified with sterols (as contained in the cocoa creams) provide new dietary therapeutic perspectives. NCT00511420.
  7. Calkin A, Tontonoz P. Transcriptional integration of metabolism by the nuclear sterol-activated receptors LXR and FXR. Nat Rev Mol Cell Biol. 2012;13:213-24 pubmed publisher
    ..Mounting evidence indicates that these nuclear receptors have essential roles, not only in the regulation of cholesterol and bile acid metabolism but also in the integration of sterol, fatty acid and glucose metabolism. ..
  8. Iborra R, Machado Lima A, Castilho G, Nunes V, Abdalla D, Nakandakare E, et al. Advanced glycation in macrophages induces intracellular accumulation of 7-ketocholesterol and total sterols by decreasing the expression of ABCA-1 and ABCG-1. Lipids Health Dis. 2011;10:172 pubmed publisher
    ..of intracellular AGE) in macrophages overloaded with oxidized LDL and incubated with HDL alone or HDL plus LXR agonist (T0901317) in: 1) the intracellular content of oxysterols and total sterols and 2) the contents of ABCA-1 and ABCG-1.
  9. Marinho P, Simas N, Kuster R, Duarte R, Fracalanzza S, Ferreira D, et al. Antibacterial activity and cytotoxicity analysis of halistanol trisulphate from marine sponge Petromica citrina. J Antimicrob Chemother. 2012;67:2396-400 pubmed publisher
    ..We performed a chemical and biological characterization of the antibiotic substance from P. citrina and investigated its mode of action on Staphylococcus aureus cells...
  10. Gylling H, Hallikainen M, Simonen P, Miettinen H, Nissinen M, Miettinen T. Serum and lipoprotein sitostanol and non-cholesterol sterols after an acute dose of plant stanol ester on its long-term consumption. Eur J Nutr. 2012;51:615-22 pubmed publisher
  11. Putra M, Bavestrello G, Cerrano C, Renga B, D Amore C, Fiorucci S, et al. Polyhydroxylated sterols from the Indonesian soft coral Sinularia sp. and their effect on farnesoid X-activated receptor. Steroids. 2012;77:433-40 pubmed publisher
    ..resulted in the isolation of three known (1, 2 and 7) and five new (3-6 and 8) sterols, characterized by either 24-methylcholestane or gorgostane skeletons...
  12. Baardman M, Erwich J, Berger R, Hofstra R, Kerstjens Frederikse W, Lutjohann D, et al. The origin of fetal sterols in second-trimester amniotic fluid: endogenous synthesis or maternal-fetal transport?. Am J Obstet Gynecol. 2012;207:202.e19-25 pubmed publisher
    ..To gain more insight into the origin of the fetal cholesterol pool in early human pregnancy, we determined cholesterol and its precursors in the amniotic fluid of uncomplicated, singleton human pregnancies...
  13. Zerenturk E, Sharpe L, Brown A. Sterols regulate 3?-hydroxysterol ?24-reductase (DHCR24) via dual sterol regulatory elements: cooperative induction of key enzymes in lipid synthesis by Sterol Regulatory Element Binding Proteins. Biochim Biophys Acta. 2012;1821:1350-60 pubmed publisher
    ..How this enzyme is regulated transcriptionally by sterols is currently unclear...
  14. Georgiev A, Sullivan D, Kersting M, Dittman J, Beh C, Menon A. Osh proteins regulate membrane sterol organization but are not required for sterol movement between the ER and PM. Traffic. 2011;12:1341-55 pubmed publisher
    ..of radiolabeled ergosterol at the PM was assessed in isolated PM-enriched fractions as well as by extracting sterols from intact cells with methyl-?-cyclodextrin...
  15. Liu T, Lu X, Tang H, Zhang M, Wang P, Sun P, et al. 3?,5?,6?-Oxygenated sterols from the South China Sea gorgonian Muriceopsis flavida and their tumor cell growth inhibitory activity and apoptosis-inducing function. Steroids. 2013;78:108-14 pubmed publisher
    ..Methylation of 5-OH contributed a little to the activity. ..
  16. Shin G, Veen M, Stahl U, Lang C. Overexpression of genes of the fatty acid biosynthetic pathway leads to accumulation of sterols in Saccharomyces cerevisiae. Yeast. 2012;29:371-83 pubmed publisher
    ..7), while sterols and squalene were increased (factor of 1.5)...
  17. Permeh P, Saeidnia S, Mashinchian Moradi A, Gohari A. Sterols from Sargassum oligocystum, a brown algae from the Persian Gulf, and their bioactivity. Nat Prod Res. 2012;26:774-7 pubmed publisher
    ..In this study, the cytotoxic effects of this algae on brine shrimp larvae were evaluated and the main sterols of the algae identified...
  18. Perrat E, Couzinet Mossion A, Fossi Tankoua O, Amiard Triquet C, Wielgosz Collin G. Variation of content of lipid classes, sterols and fatty acids in gonads and digestive glands of Scrobicularia plana in relation to environment pollution levels. Ecotoxicol Environ Saf. 2013;90:112-20 pubmed publisher
    ..Fatty acids and sterols were then analyzed by gas chromatography-mass spectrometry...
  19. Sáenz J, Sezgin E, Schwille P, Simons K. Functional convergence of hopanoids and sterols in membrane ordering. Proc Natl Acad Sci U S A. 2012;109:14236-40 pubmed publisher
    ..The formation of a liquid-ordered phase depends crucially on the ordering properties of sterols. However, it is not known whether this capacity exists in organisms that lack sterols, such as bacteria...
  20. Wilcock B, Endo M, Uno B, Burke M. C2'-OH of amphotericin B plays an important role in binding the primary sterol of human cells but not yeast cells. J Am Chem Soc. 2013;135:8488-91 pubmed publisher
    ..structural model predicts that the C2' hydroxyl group on the mycosamine appendage is critical for binding both sterols. To test this, the C2'-OH was synthetically deleted, and the sterol binding capacity of the resulting derivative, ..
  21. Furtula V, Osachoff H, Derksen G, Juahir H, Colodey A, Chambers P. Inorganic nitrogen, sterols and bacterial source tracking as tools to characterize water quality and possible contamination sources in surface water. Water Res. 2012;46:1079-92 pubmed publisher
    ..019 mg/L N). Gas chromatography mass spectrometry single ion monitoring (GC-MS SIM) analysis of 18 sterols showed that five fecal sterols (coprostanol, episoprostanol, cholesterol, cholestanol, desmosterol) were detected ..
  22. De Marino S, Ummarino R, D Auria M, Chini M, Bifulco G, D Amore C, et al. 4-Methylenesterols from Theonella swinhoei sponge are natural pregnane-X-receptor agonists and farnesoid-X-receptor antagonists that modulate innate immunity. Steroids. 2012;77:484-95 pubmed publisher
    ..These results pave the way to development of a dual PXR agonist/FXR antagonist with a robust immunomodulatory activity and endowed with the ability to modulate the expression of bile acid-regulated genes in the liver...
  23. Giner J, Wikfors G. "Dinoflagellate Sterols" in marine diatoms. Phytochemistry. 2011;72:1896-901 pubmed publisher
    Sterol compositions for three diatom species, recently shown to contain sterols with side chains typically found in dinoflagellates, were determined by HPLC and ¹H NMR spectroscopic analyses...
  24. Krawczyk M, Lutjohann D, Schirin Sokhan R, Villarroel L, Nervi F, Pimentel F, et al. Phytosterol and cholesterol precursor levels indicate increased cholesterol excretion and biosynthesis in gallstone disease. Hepatology. 2012;55:1507-17 pubmed publisher
    ..Serum sterols were measured by gas chromatography / mass spectrometry...
  25. Yi C, Fu M, Cao X, Tong S, Zheng Q, Firempong C, et al. Enhanced oral bioavailability and tissue distribution of a new potential anticancer agent, Flammulina velutipes sterols, through liposomal encapsulation. J Agric Food Chem. 2013;61:5961-71 pubmed publisher
    This study innovatively investigated the anticancer effect of Flammulina velutipes sterols (FVSs), the in vivo pharmacokinetics, and the tissue distribution of FVS-loaded liposomes. The FVS consisting of mainly 54.8% ergosterol and 27...
  26. Zang Y, Xiong J, Zhai W, Cao L, Zhang S, Tang Y, et al. Fomentarols A-D, sterols from the polypore macrofungus Fomes fomentarius. Phytochemistry. 2013;92:137-45 pubmed publisher
    Four (1-4) hitherto unknown and seven (5-11) known ergostane-type sterols were isolated from the EtOH extract of the dried fruiting bodies of the polypore macrofungus Fomes fomentarius...
  27. Spann N, Glass C. Sterols and oxysterols in immune cell function. Nat Immunol. 2013;14:893-900 pubmed publisher
    ..Such findings have raised many new questions about the production of endogenous bioactive sterols and oxysterols and their mechanisms of action in the immune system.
  28. Grillitsch K, Connerth M, Köfeler H, Arrey T, Rietschel B, Wagner B, et al. Lipid particles/droplets of the yeast Saccharomyces cerevisiae revisited: lipidome meets proteome. Biochim Biophys Acta. 2011;1811:1165-76 pubmed publisher
    ..esters (SE) are stored in so-called lipid particles/droplets (LP) as biologically inert form of fatty acids and sterols. To understand LP structure and function in more detail we investigated the molecular equipment of this ..
  29. Igarashi F, Hikiba J, Ogihara M, Nakaoka T, Suzuki M, Kataoka H. A highly specific and sensitive quantification analysis of the sterols in silkworm larvae by high performance liquid chromatography-atmospheric pressure chemical ionization-tandem mass spectrometry. Anal Biochem. 2011;419:123-32 pubmed publisher
    The biochemical quantification of sterols in insects has been difficult because only small amounts of tissues can be obtained from insect bodies and because sterol metabolites are structurally related...
  30. Renga B, Mencarelli A, D Amore C, Cipriani S, D Auria M, Sepe V, et al. Discovery that theonellasterol a marine sponge sterol is a highly selective FXR antagonist that protects against liver injury in cholestasis. PLoS ONE. 2012;7:e30443 pubmed publisher
    ..FXR antagonism in vivo results in a positive modulation of MRP4 expression in the liver and is a feasible strategy to target obstructive cholestasis. ..
  31. Matysik S, Klünemann H, Schmitz G. Gas chromatography-tandem mass spectrometry method for the simultaneous determination of oxysterols, plant sterols, and cholesterol precursors. Clin Chem. 2012;58:1557-64 pubmed publisher
    Cholesterol precursors and plant sterols have considerable potential as plasma biomarkers in several disorders of sterol metabolism and intestinal sterol absorption...
  32. Espiritu R, Matsumori N, Murata M, Nishimura S, Kakeya H, Matsunaga S, et al. Interaction between the marine sponge cyclic peptide theonellamide A and sterols in lipid bilayers as viewed by surface plasmon resonance and solid-state (2)H nuclear magnetic resonance. Biochemistry. 2013;52:2410-8 pubmed publisher
    ..This study demonstrates that TNM-A directly recognizes the 3?-OH moiety of sterols, which greatly facilitates its binding to bilayer membranes.
  33. Pan L, Lezama Davila C, Isaac Márquez A, Calomeni E, Fuchs J, Satoskar A, et al. Sterols with antileishmanial activity isolated from the roots of Pentalinon andrieuxii. Phytochemistry. 2012;82:128-35 pubmed publisher polyoxygenated pregnane sterol glycoside, pentalinonside (2), together with 18 known compounds, including 14 sterols (3-16), three coumarins (17-19), and a triterpene (20), were isolated from a n-hexane partition of a methanol ..
  34. Alfaro G, Johansen J, Dighe S, Duamel G, Kozminski K, Beh C. The sterol-binding protein Kes1/Osh4p is a regulator of polarized exocytosis. Traffic. 2011;12:1521-36 pubmed publisher
    ..These results support a model in which Osh4p acts as a sterol-dependent regulator of polarized vesicle transport, as opposed to being a sterol-transfer protein. ..
  35. Diezi T, KWON G. Amphotericin B/sterol co-loaded PEG-phospholipid micelles: effects of sterols on aggregation state and hemolytic activity of amphotericin B. Pharm Res. 2012;29:1737-44 pubmed publisher
    To elucidate the effect of sterols on the aggregation of amphotericin B (AmB) in PEG-phospholipid micelles and its consequences on the hemolytic activity of AmB...
  36. Birk J, Dippold M, Wiesenberg G, Glaser B. Combined quantification of faecal sterols, stanols, stanones and bile acids in soils and terrestrial sediments by gas chromatography-mass spectrometry. J Chromatogr A. 2012;1242:1-10 pubmed publisher
    Faeces incorporation can alter the concentration patterns of stanols, stanones, ?(5)-sterols and bile acids in soils and terrestrial sediments...
  37. Kitson S, Mullen W, Cogdell R, Bill R, Fraser N. GPCR production in a novel yeast strain that makes cholesterol-like sterols. Methods. 2011;55:287-92 pubmed publisher
    ..Thus far, we have created a modified strain that makes cholesterol-like sterols which has an increased capacity to make G-protein coupled receptors compared to control yeast.
  38. Basen T, Rothhaupt K, Martin Creuzburg D. Absence of sterols constrains food quality of cyanobacteria for an invasive freshwater bivalve. Oecologia. 2012;170:57-64 pubmed publisher
    ..Here, we investigated the role of sterols in determining the food quality of cyanobacteria for the invasive clam Corbicula fluminea, which has become a ..
  39. Nachtergaele S, Mydock L, Krishnan K, Rammohan J, Schlesinger P, Covey D, et al. Oxysterols are allosteric activators of the oncoprotein Smoothened. Nat Chem Biol. 2012;8:211-20 pubmed publisher
    ..Our work suggests that these enigmatic sterols, which have multiple effects on cell physiology, may act as ligands for signaling receptors and provides a ..
  40. Keber R, Rozman D, Horvat S. Sterols in spermatogenesis and sperm maturation. J Lipid Res. 2013;54:20-33 pubmed publisher
    ..However, the exact role of sterols in male reproduction is unknown...
  41. Gill S, Chow R, Brown A. Sterol regulators of cholesterol homeostasis and beyond: the oxysterol hypothesis revisited and revised. Prog Lipid Res. 2008;47:391-404 pubmed publisher
    ..Some (oxy)sterols can accelerate the degradation of the key cholesterol biosynthetic enzyme, HMG-CoA reductase, and/or serve as ..
  42. Walker B, Manhanke N, Stine K. Comparison of the interaction of tomatine with mixed monolayers containing phospholipid, egg sphingomyelin, and sterols. Biochim Biophys Acta. 2008;1778:2244-57 pubmed publisher
    ..Brewster angle microscopy (BAM) reveals further evidence for formation of suspected islands of tomatine + cholesterol complexes upon interaction with mixed monolayers of lipid and sterol. ..
  43. Potocka A, Zimowski J. Metabolism of conjugated sterols in eggplant. Part 2. Phospholipid : steryl glucoside acyltransferase. Acta Biochim Pol. 2008;55:135-40 pubmed
  44. Sepe V, Bifulco G, Renga B, D Amore C, Fiorucci S, Zampella A. Discovery of sulfated sterols from marine invertebrates as a new class of marine natural antagonists of farnesoid-X-receptor. J Med Chem. 2011;54:1314-20 pubmed publisher
    ..This compound is a major tool to explore the effect of FXR inhibition in pharmacological settings. ..
  45. Gylling H, Hallikainen M, Rajaratnam R, Simonen P, Pihlajamaki J, Laakso M, et al. The metabolism of plant sterols is disturbed in postmenopausal women with coronary artery disease. Metabolism. 2009;58:401-7 pubmed publisher
    In postmenopausal coronary artery disease (CAD) women, serum plant sterols are elevated...
  46. Wang Y, Sousa K, Bodin K, Theofilopoulos S, Sacchetti P, Hornshaw M, et al. Targeted lipidomic analysis of oxysterols in the embryonic central nervous system. Mol Biosyst. 2009;5:529-41 pubmed publisher
  47. DiGirolamo J, Li X, Jacob M, Clark A, Ferreira D. Reversal of fluconazole resistance by sulfated sterols from the marine sponge Topsentia sp. J Nat Prod. 2009;72:1524-8 pubmed publisher
    Bioassay-guided fractionation of the extract of Topsentia sp. led to the identification of two new sulfated sterols, geodisterol-3-O-sulfite (1) and 29-demethylgeodisterol-3-O-sulfite (2), the active constituents reversing efflux pump-..
  48. De Marino S, Sepe V, D Auria M, Bifulco G, Renga B, Petek S, et al. Towards new ligands of nuclear receptors. Discovery of malaitasterol A, an unique bis-secosterol from marine sponge Theonella swinhoei. Org Biomol Chem. 2011;9:4856-62 pubmed publisher
    ..The biological characterization of malaitasterol A provided evidence that this compound is a potent agonist of pregnane-X-receptor and its putative binding mode to PXR has been obtained through docking calculations...
  49. Gulati S, Liu Y, Munkacsi A, Wilcox L, Sturley S. Sterols and sphingolipids: dynamic duo or partners in crime?. Prog Lipid Res. 2010;49:353-65 pubmed publisher which eukaryotic cells respond to their environments is by optimizing the composition and proportions of sterols and sphingolipids in membranes...
  50. Pais S, Miñones J, Conde O, Dynarowicz Łatka P. Interactions between membrane sterols and phospholipids in model mammalian and fungi cellular membranes--a Langmuir monolayer study. Biophys Chem. 2009;140:69-77 pubmed publisher
    ..For each system, two different concentrations of the sterols were used, 13 and 30%, corresponding to the range of concentration found in various natural membranes...
  51. Chaturvedi P, Misra P, Tuli R. Sterol glycosyltransferases--the enzymes that modify sterols. Appl Biochem Biotechnol. 2011;165:47-68 pubmed publisher
    b>Sterols are important components of cell membranes, hormones, signalling molecules and defense-related biotic and abiotic chemicals...
  52. McDonald J, Thompson B, McCrum E, Russell D. Extraction and analysis of sterols in biological matrices by high performance liquid chromatography electrospray ionization mass spectrometry. Methods Enzymol. 2007;432:145-70 pubmed
    ..liquid chromatography mass spectrometry (HPLC-MS) method that allows the identification and quantitation of sterols in mammalian cells and tissues...
  53. Hong Y, Turowski M, Lin J, Yokoyama W. Simultaneous characterization of bile acid, sterols, and determination of acylglycerides in feces from soluble cellulose-fed hamsters using HPLC with evaporative light-scattering detection and APCI-MS. J Agric Food Chem. 2007;55:9750-7 pubmed
    ..identified using this method includes two secondary bile acids, deoxycholic acid, lithocholic acid, and neutral sterols including cholesterol, coprostanol, stigmastanol, and sitosterol...