glycosylation

Summary

Summary: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

Top Publications

  1. pmc O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding
    Takako Yoshida-Moriguchi
    Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 327:88-92. 2010
  2. ncbi Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody
    P D Kwong
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nature 393:648-59. 1998
  3. pmc Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
    A Trkola
    Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA
    J Virol 70:1100-8. 1996
  4. pmc Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line
    Philip J Reeves
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13419-24. 2002
  5. ncbi Glycosylation in cellular mechanisms of health and disease
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive MC0625, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 126:855-67. 2006
  6. doi N-linked protein glycosylation in the ER
    Markus Aebi
    Department of Biology, Institute of Microbiology, Zurich, Switzerland
    Biochim Biophys Acta 1833:2430-7. 2013
  7. doi Glycosyltransferases: structures, functions, and mechanisms
    L L Lairson
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    Annu Rev Biochem 77:521-55. 2008
  8. ncbi The impact of glycosylation on the biological function and structure of human immunoglobulins
    James N Arnold
    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Annu Rev Immunol 25:21-50. 2007
  9. pmc The mannose-dependent epitope for neutralizing antibody 2G12 on human immunodeficiency virus type 1 glycoprotein gp120
    Rogier W Sanders
    Dept of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Virol 76:7293-305. 2002
  10. pmc A multifunctional mannosyltransferase family in Candida albicans determines cell wall mannan structure and host-fungus interactions
    Hector M Mora-Montes
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, UK
    J Biol Chem 285:12087-95. 2010

Research Grants

  1. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2013
  2. C5a as an Anti-HIV Microbicidal Candidate
    PHILIPPE ANDRE GALLAY; Fiscal Year: 2013
  3. Novel Bioconjugate Vaccines to Prevent Staphylococcus aureus Infection
    JEAN CLAIRE LEE; Fiscal Year: 2013
  4. Use of Phosphodiesterase Inhibitors to Evaluate the Pathobiology of CF
    JENNIFER L TAYLOR-COUSAR; Fiscal Year: 2013
  5. Phospholipid flip-flop in biogenic membranes
    Anant K Menon; Fiscal Year: 2013
  6. Misregulation of receptor tyrosine kinase signaling in PKD
    Jordan A Kreidberg; Fiscal Year: 2013
  7. Lactoferrin for Immunomodulation of Systemic Inflammatory Response Syndrome
    Marian L Kruzel; Fiscal Year: 2011
  8. Antigen receptor inputs: linking structural, molecular, and cellular responses
    Jonathan P Schneck; Fiscal Year: 2012
  9. Mechanistic Studies of HCV E2
    Joseph Marcotrigiano; Fiscal Year: 2013
  10. N-Glycosylation And ER Stress
    MARK LEHRMAN; Fiscal Year: 2012

Detail Information

Publications416 found, 100 shown here

  1. pmc O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding
    Takako Yoshida-Moriguchi
    Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 327:88-92. 2010
    ..These findings expand our understanding of the mechanisms that underlie congenital muscular dystrophy...
  2. ncbi Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody
    P D Kwong
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nature 393:648-59. 1998
    ..Our results provide a framework for understanding the complex biology of HIV entry into cells and should guide efforts to intervene...
  3. pmc Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
    A Trkola
    Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA
    J Virol 70:1100-8. 1996
    ..consistent with this, antibodies able to block 2G12 binding to recombinant gp120 were not detected in significant quantities in 16 HIV-positive human serum samples...
  4. pmc Structure and function in rhodopsin: high-level expression of rhodopsin with restricted and homogeneous N-glycosylation by a tetracycline-inducible N-acetylglucosaminyltransferase I-negative HEK293S stable mammalian cell line
    Philip J Reeves
    Departments of Biology and Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Proc Natl Acad Sci U S A 99:13419-24. 2002
    ....
  5. ncbi Glycosylation in cellular mechanisms of health and disease
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive MC0625, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 126:855-67. 2006
    b>Glycosylation produces an abundant, diverse, and highly regulated repertoire of cellular glycans that are frequently attached to proteins and lipids...
  6. doi N-linked protein glycosylation in the ER
    Markus Aebi
    Department of Biology, Institute of Microbiology, Zurich, Switzerland
    Biochim Biophys Acta 1833:2430-7. 2013
    N-linked protein glycosylation in the endoplasmic reticulum (ER) is a conserved two phase process in eukaryotic cells...
  7. doi Glycosyltransferases: structures, functions, and mechanisms
    L L Lairson
    Department of Chemistry, University of British Columbia, Vancouver, BC, Canada
    Annu Rev Biochem 77:521-55. 2008
    ..A mechanism involving a short-lived oxocarbenium ion intermediate now seems the most likely, with the leaving phosphate serving as the base...
  8. ncbi The impact of glycosylation on the biological function and structure of human immunoglobulins
    James N Arnold
    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom
    Annu Rev Immunol 25:21-50. 2007
    ..The models were constructed by combining glycan sequencing data with oligosaccharide linkage and dynamics information from the Glycobiology Institute experimental database and protein structural data from "The Protein Data Bank."..
  9. pmc The mannose-dependent epitope for neutralizing antibody 2G12 on human immunodeficiency virus type 1 glycoprotein gp120
    Rogier W Sanders
    Dept of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Virol 76:7293-305. 2002
    ....
  10. pmc A multifunctional mannosyltransferase family in Candida albicans determines cell wall mannan structure and host-fungus interactions
    Hector M Mora-Montes
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, UK
    J Biol Chem 285:12087-95. 2010
    ..b>Glycosylation of cell wall proteins involves the activities of families of endoplasmic reticulum and Golgi-located glycosyl ..
  11. doi Dystroglycanopathies: coming into focus
    Caroline Godfrey
    Dubowitz Neuromuscular Centre, UCL Institute of Child Health and the Great Ormond Street Hospital for Children, Guilford Street, University College London, United Kingdom
    Curr Opin Genet Dev 21:278-85. 2011
    A common group of muscular dystrophies is associated with the aberrant glycosylation of α-dystroglycan...
  12. pmc Effects of glycosylation on the stability of protein pharmaceuticals
    Ricardo J Solá
    Laboratory for Applied Biochemistry and Biotechnology, Department of Chemistry, University of Puerto Rico, Rio Piedras Campus, Facundo Bueso Bldg, Lab 215, PO Box 23346, San Juan, Puerto Rico 00931 3346
    J Pharm Sci 98:1223-45. 2009
    ..effect that glycans have in increasing the overall stability of glycoproteins, rational manipulation of the glycosylation parameters through glycoengineering could become a promising approach to improve both the in vitro and in vivo ..
  13. pmc Mutations in ISPD cause Walker-Warburg syndrome and defective glycosylation of α-dystroglycan
    Tony Roscioli
    Department of Human Genetics, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Nat Genet 44:581-5. 2012
    ..by complex eye and brain abnormalities with congenital muscular dystrophy (CMD) and aberrant a-dystroglycan glycosylation. Here we report mutations in the ISPD gene (encoding isoprenoid synthase domain containing) as the second most ..
  14. ncbi Envelope-constrained neutralization-sensitive HIV-1 after heterosexual transmission
    Cynthia A Derdeyn
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
    Science 303:2019-22. 2004
    ..This reveals characteristics of the envelope glycoprotein that influence HIV-1 transmission and may have implications for vaccine design...
  15. pmc Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion
    M Flamand
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, 91198 Gif sur Yvette Cedex, France
    J Virol 73:6104-10. 1999
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell.
  16. ncbi HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites
    Peter D Kwong
    Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 420:678-82. 2002
    ..Because this solution is available for cell-surface receptors but not for most antibodies, conformational masking enables HIV-1 to maintain receptor binding and simultaneously to resist neutralization...
  17. doi Precision mapping of an in vivo N-glycoproteome reveals rigid topological and sequence constraints
    Dorota F Zielinska
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried D 82152, Germany
    Cell 141:897-907. 2010
    N-linked glycosylation is a biologically important protein modification, but only a small fraction of modification sites have been mapped...
  18. ncbi Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells
    C K Leonard
    Department of Medicinal Chemistry, Genentech, Inc, South San Francisco, California 94080
    J Biol Chem 265:10373-82. 1990
    ..structure of the protein, to assign intrachain disulfide bonds, and to characterize potential sites for N-glycosylation. All of the tryptic peptides identified were consistent with the primary structure predicted from the cDNA ..
  19. pmc Computational identification of novel biochemical systems involved in oxidation, glycosylation and other complex modifications of bases in DNA
    Lakshminarayan M Iyer
    National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA
    Nucleic Acids Res 41:7635-55. 2013
    ..These results open opportunities for the investigation of the biology of these systems and their use in biotechnology. ..
  20. pmc ISPD loss-of-function mutations disrupt dystroglycan O-mannosylation and cause Walker-Warburg syndrome
    Tobias Willer
    Department of Molecular Physiology and Biophysics, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, Iowa, USA
    Nat Genet 44:575-80. 2012
    ..Finally, we show that recessive mutations in ISPD abolish the initial step in laminin-binding glycan synthesis by disrupting dystroglycan O-mannosylation. This establishes a new mechanism for WWS pathophysiology...
  21. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  22. ncbi Glycosylation and the immune system
    P M Rudd
    The Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Science 291:2370-6. 2001
    ..For example, in rheumatoid arthritis, an autoimmune disease, agalactosylated glycoforms of aggregated immunoglobulin G may induce association with the mannose-binding lectin and contribute to the pathology...
  23. doi STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein
    Takashi Sato
    Department of Molecular Medicine, Global COE Cell Fate Regulation Research and Education Unit, Graduate School of Pharmaceutical Sciences, Kumamoto University, 5 1 Oe honmachi, Kumamoto 862 0973, Japan
    Mol Cell 47:99-110. 2012
    ..We report here the cryptic N-glycosylation site as a recognition signal for unfolding of a natively nonglycosylated protein, transthyretin (TTR), ..
  24. ncbi Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites
    Karin Julenius
    Center for Biological Sequence Analysis, BioCentrum, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Glycobiology 15:153-64. 2005
    O-GalNAc-glycosylation is one of the main types of glycosylation in mammalian cells...
  25. pmc Glycans on influenza hemagglutinin affect receptor binding and immune response
    Cheng Chi Wang
    Genomics Research Center, Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, Taipei 115, Taiwan
    Proc Natl Acad Sci U S A 106:18137-42. 2009
    ..To elucidate the role of HA glycosylation in this important interaction, various defined HA glycoforms were prepared, and their binding affinity and ..
  26. pmc Broad neutralization coverage of HIV by multiple highly potent antibodies
    Laura M Walker
    Department of Immunology and Microbial Science and IAVI Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, California 92037, USA
    Nature 477:466-70. 2011
    ....
  27. pmc The expanding horizons of asparagine-linked glycosylation
    Angelyn Larkin
    Department of Chemistry Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Biochemistry 50:4411-26. 2011
    Asparagine-linked glycosylation involves the sequential assembly of an oligosaccharide onto a polyisoprenyl donor, followed by the en bloc transfer of the glycan to particular asparagine residues within acceptor proteins...
  28. pmc Dystroglycan function requires xylosyl- and glucuronyltransferase activities of LARGE
    Kei Ichiro Inamori
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 335:93-6. 2012
    ..This modification allowed α-DG to bind laminin-G domain-containing ECM ligands...
  29. pmc Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG Fc
    Robert M Anthony
    Laboratory of Molecular Genetics and Immunology, Rockefeller University, New York, NY 10021, USA
    Science 320:373-6. 2008
    ..This therapeutic molecule precisely defines the biologically active component of IVIG and helps guide development of an IVIG replacement with improved activity and availability...
  30. pmc Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens
    Katie J Doores
    Department of Immunology and Microbial Science and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:13800-5. 2010
    ..In stark contrast to recombinant gp120, which shows extensive exposure to cellular glycosylation enzymes (>70% complex type glycans), the native envelope shows barely detectable processing beyond the ..
  31. doi Protein glycosylation in bacteria: sweeter than ever
    Harald Nothaft
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Nat Rev Microbiol 8:765-78. 2010
    Investigations into bacterial protein glycosylation continue to progress rapidly...
  32. pmc N-linked glycosylation of west nile virus envelope proteins influences particle assembly and infectivity
    Sheri L Hanna
    Department of Microbiology, University of Pennsylvania, 225 Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104, USA
    J Virol 79:13262-74. 2005
    ..While the prM protein of all WNV strains contains a single N-linked glycosylation site, not all strains contain an N-linked site in the E protein...
  33. doi Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Plant Biotechnol J 6:392-402. 2008
    ..using plants as a production system for therapeutic proteins is their inability to perform authentic human N-glycosylation (i.e. the presence of beta1,2-xylosylation and core alpha1,3-fucosylation)...
  34. pmc Neutralizing antibody responses in acute human immunodeficiency virus type 1 subtype C infection
    E S Gray
    AIDS Virus Research Unit, National Institute for Communicable Diseases, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 81:6187-96. 2007
    ..The magnitude of this response was associated with shorter V1-to-V5 envelope lengths and fewer glycosylation sites, particularly in the V1-V2 region...
  35. pmc The pathophysiology of IgA nephropathy
    Hitoshi Suzuki
    Department of Internal Medicine, Division of Nephrology, Juntendo University Faculty of Medicine, Tokyo, Japan
    J Am Soc Nephrol 22:1795-803. 2011
    ..This glycosylation aberrancy is, however, not sufficient to induce renal injury...
  36. ncbi A time- and cost-efficient system for high-level protein production in mammalian cells
    A Radu Aricescu
    Cancer Research UK Receptor Structure Research Group, Division of Structural Biology, Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, England
    Acta Crystallogr D Biol Crystallogr 62:1243-50. 2006
    ..high protein yield, straightforward purification, selenomethionine incorporation and control of N-linked glycosylation. The system is suitable for use in conventional laboratories or can be implemented in a medium- or high-..
  37. pmc Mammalian glycosylation in immunity
    Jamey D Marth
    Department of Cellular and Molecular Medicine, The Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093, USA
    Nat Rev Immunol 8:874-87. 2008
    b>Glycosylation produces a diverse and abundant repertoire of glycans, which are collectively known as the glycome...
  38. pmc Beta-N-acetylglucosamine (O-GlcNAc) is part of the histone code
    Kaoru Sakabe
    Department of Biological Chemistry, The Johns Hopkins University, Baltimore, MD 21205, USA
    Proc Natl Acad Sci U S A 107:19915-20. 2010
    ..Finally, we show that histone O-GlcNAcylation changes during mitosis and with heat shock. Taken together, these data show that O-GlcNAc cycles dynamically on histones and can be considered part of the histone code...
  39. doi Moss-based production of asialo-erythropoietin devoid of Lewis A and other plant-typical carbohydrate determinants
    Juliana Parsons
    Plant Biotechnology, University of Freiburg, Freiburg, Germany
    Plant Biotechnol J 10:851-61. 2012
    ..However, as the majority of biopharmaceuticals are glycoproteins, plant-specific N-glycosylation has to be taken into consideration...
  40. doi Oligosaccharyltransferase: the central enzyme of N-linked protein glycosylation
    Elisabeth Mohorko
    Department of Biology, Institute of Molecular Biology and Biophysics, ETH Zurich, Schafmatt 20, CH, 8093, Zurich, Switzerland
    J Inherit Metab Dis 34:869-78. 2011
    N-linked glycosylation is one of the most abundant modifications of proteins in eukaryotic organisms...
  41. doi Sweet-talk: role of host glycosylation in bacterial pathogenesis of the gastrointestinal tract
    A P Moran
    Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland
    Gut 60:1412-25. 2011
    b>Glycosylation is a key modification of proteins and lipids and is involved in most intermolecular and intercellular interactions...
  42. doi A novel role for the IgG Fc glycan: the anti-inflammatory activity of sialylated IgG Fcs
    Robert M Anthony
    The Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York, NY 10065, USA
    J Clin Immunol 30:S9-14. 2010
    ..This leads to upregulated surface expression of the inhibitory FcR, FcgammaRIIb, on inflammatory cells, thereby attenuating autoantibody-initiated inflammation...
  43. pmc Glycosylation regulates pannexin intermixing and cellular localization
    Silvia Penuela
    Department of Anatomy and Cell Biology, University of Western Ontario, London, Ontario N6A 5C1, Canada
    Mol Biol Cell 20:4313-23. 2009
    ..assays revealed that Panx1 can interact with Panx2 and to a lesser extent, with Panx3 in a glycosylation-dependent manner. Panx2 strongly interacts with the core and high-mannose species of Panx1 but not with Panx3...
  44. pmc Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation
    Jeremy A Iwashkiw
    Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    PLoS Pathog 8:e1002758. 2012
    ..In this work we show that A. baumannii ATCC 17978 possesses an O-glycosylation system responsible for the glycosylation of multiple proteins...
  45. ncbi The acute phase protein alpha1-acid glycoprotein: a model for altered glycosylation during diseases
    Fabrizio Ceciliani
    Dipartimento di Patologia Animale, Igiene e Sanita Pubblica Veterinaria, Universita di Milano, Sezione di Patologia Generale e Parassitologia, Via Celoria 10 20133, Milano, Italy
    Curr Protein Pept Sci 8:91-108. 2007
    b>Glycosylation is one of the most important post-translational modifications of proteins, and has been widely acknowledged as one of the most important ways to modulate both protein function and lifespan...
  46. pmc N-glycosylation of colorectal cancer tissues: a liquid chromatography and mass spectrometry-based investigation
    Crina I A Balog
    Biomolecular Mass Spectrometry Unit, Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands
    Mol Cell Proteomics 11:571-85. 2012
    ..Using partial least squares discriminant analysis to investigate the N-glycosylation changes in colorectal cancer, an excellent separation and prediction ability were observed for both HILIC-HPLC ..
  47. doi Pandemic H1N1 influenza A viruses are resistant to the antiviral activities of innate immune proteins of the collectin and pentraxin superfamilies
    Emma R Job
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
    J Immunol 185:4284-91. 2010
    ..of H1N1 viruses to infect human respiratory epithelial cells in a manner that correlated with the degree of glycosylation in the globular head of the hemagglutinin...
  48. pmc Aberrant glycosylation of IgA1 is inherited in both pediatric IgA nephropathy and Henoch-Schönlein purpura nephritis
    Krzysztof Kiryluk
    Division of Nephrology, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Kidney Int 80:79-87. 2011
    ..Thus, serum galactose-deficient IgA1 levels are highly inherited in pediatric patients with IgAN and HSPN, providing support for another shared pathogenic link between these disorders...
  49. ncbi Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans
    Steven Bates
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    J Biol Chem 281:90-8. 2006
    ..However, tissue burdens for the Caoch1delta null mutant were similar to control strains with normal N-glycosylation, suggesting the host-fungal interaction was altered such that high burdens were tolerated...
  50. pmc Candida albicans cell wall glycosylation may be indirectly required for activation of epithelial cell proinflammatory responses
    Celia Murciano
    Department of Oral Immunology, Floor 28, Tower Wing, King s College London Dental Institute, King s College London, London SE1 9RT, United Kingdom
    Infect Immun 79:4902-11. 2011
    ..host recognition and immune activation in myeloid cells, we sought to determine whether β-glucan and N- or O-glycosylation was important in activating the MAPK/MKP1/c-Fos hypha-mediated response mechanism and proinflammatory ..
  51. ncbi Statistical analysis of the protein environment of N-glycosylation sites: implications for occupancy, structure, and folding
    Andrei J Petrescu
    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U K
    Glycobiology 14:103-14. 2004
    ..analysis to the glycan-protein linkage, and the peptide primary, secondary, and tertiary structures around N-glycosylation sites...
  52. pmc Characterization of the PMT gene family in Cryptococcus neoformans
    Sven D Willger
    Institut fur Mikrobiologie, Molekulare Mykologie, Heinrich Heine Universitat, Dusseldorf, Germany
    PLoS ONE 4:e6321. 2009
    Protein-O-mannosyltransferases (Pmt's) catalyze the initial step of protein-O-glycosylation, the addition of mannose residues to serine or threonine residues of target proteins.
  53. pmc Glycosylation in immune cell trafficking
    Markus Sperandio
    Walter Brendel Center of Experimental Medicine, Ludwig Maximilians Universitat, Munich, Germany
    Immunol Rev 230:97-113. 2009
    ..Advancing the knowledge on glycan function into appropriate pathology models is likely to suggest interesting new therapeutic strategies in the treatment of immune- and inflammation-mediated diseases...
  54. ncbi Mutations in the human LARGE gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan
    Cheryl Longman
    Dubowitz Neuromuscular Centre, Imperial College, Hammersmith Campus, London, UK
    Hum Mol Genet 12:2853-61. 2003
    ..This is the first description of mutations in the human LARGE gene and we propose to name this new disorder MDC1D...
  55. pmc Inhibition of Lassa and Marburg virus production by tetherin
    Toshie Sakuma
    First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277 0882, Japan
    J Virol 83:2382-5. 2009
    ..by viral matrix proteins of Lassa virus or Marburg virus was markedly inhibited by tetherin and that N-linked glycosylation of tetherin was dispensable for this antiviral activity...
  56. pmc Pattern recognition receptors require N-glycosylation to mediate plant immunity
    Heidrun Häweker
    Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, 50829 Cologne, Germany
    J Biol Chem 285:4629-36. 2010
    ..and FLS2, sensing bacterial translation elongation factor Tu (elf18) and flagellin (flg22), respectively, in N-glycosylation mutants...
  57. ncbi Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation
    Yoshikatsu Kaneko
    Laboratory of Molecular Genetics and Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 313:670-3. 2006
    ..This differential sialylation may provide a switch from innate anti-inflammatory activity in the steady state to generating adaptive pro-inflammatory effects upon antigenic challenge...
  58. ncbi N-glycosylation affects the molecular organization and stability of E-cadherin junctions
    Aneta Liwosz
    Department of Molecular and Cell Biology, School of Dental Medicine, Boston University, Boston, Massachusetts 02118, USA
    J Biol Chem 281:23138-49. 2006
    ..that variations in AJ stability were accompanied by quantitative and qualitative changes in E-cadherin N-glycosylation. To further examine the role of N-glycans in AJ function, we generated E-cadherin N-glycosylation variants ..
  59. doi Identification of a new family of genes involved in beta-1,2-mannosylation of glycans in Pichia pastoris and Candida albicans
    Celine Mille
    Unité de Physiopathologie des Candidoses, INSERM U799, Université de Lille 2 EA 2684, Lille 59045, France
    J Biol Chem 283:9724-36. 2008
    ..Surprisingly, depletion in mannan major beta-1,2-oligomannoside epitopes had little impact on cell wall surface beta-1,2-oligomannoside antigenic expression...
  60. ncbi Structural analysis of human IgG-Fc glycoforms reveals a correlation between glycosylation and structural integrity
    S Krapp
    Max Planck Institut fur Biochemie, Abteilung Strukturforschung, Am Klopferspitz 18a, D 82152, Martinsried, Germany
    J Mol Biol 325:979-89. 2003
    ..It is established that glycosylation of IgG-Fc is essential for recognition and activation of these ligands...
  61. pmc Human immunodeficiency virus type 1 V1-V2 envelope loop sequences expand and add glycosylation sites over the course of infection, and these modifications affect antibody neutralization sensitivity
    Manish Sagar
    Department of Medicine, Brigham and Women s Hospital, Cambridge, Massachusetts 02139, USA
    J Virol 80:9586-98. 2006
    ..Changes in the envelope variable loop sequences, particularly the extent of glycosylation, have been implicated in antibody escape...
  62. pmc Mutations in the O-mannosyltransferase gene POMT1 give rise to the severe neuronal migration disorder Walker-Warburg syndrome
    Daniel Beltran-Valero de Bernabe
    Department of Human Genetics, University Medical Centre Nijmegen, Nijmegen, The Netherlands
    Am J Hum Genet 71:1033-43. 2002
    ..The protein products of POMGnT1 and Fukutin have both been implicated in protein glycosylation. To unravel the genetic basis of WWS, we first performed a genomewide linkage analysis in 10 consanguineous ..
  63. pmc Glycosylation defining cancer malignancy: new wine in an old bottle
    Senitiroh Hakomori
    Pacific Northwest Research Institute, and Department of Pathobiology, University of Washington, Seattle, WA 98122 4327, USA
    Proc Natl Acad Sci U S A 99:10231-3. 2002
  64. pmc Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgammaRIII and antibodies lacking core fucose
    Claudia Ferrara
    Pharma Research and Early Development, Roche Glycart AG, CH 8952 Schlieren, Switzerland
    Proc Natl Acad Sci U S A 108:12669-74. 2011
    ..These findings allow us to understand the higher efficacy of therapeutic antibodies lacking the core fucose and also suggest a unique mechanism by which the immune system can regulate antibody-mediated effector functions...
  65. ncbi Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence
    Nikolaj Blom
    Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark
    Proteomics 4:1633-49. 2004
    ..We describe our experiences with the development of prediction methods for phosphorylation and glycosylation sites and the development of PTM-specific databases...
  66. ncbi Death-receptor O-glycosylation controls tumor-cell sensitivity to the proapoptotic ligand Apo2L/TRAIL
    Klaus W Wagner
    Department of Molecular Diagnostics, Genentech, Inc, 1 DNA Way, South San Francisco, California 94080, USA
    Nat Med 13:1070-7. 2007
    ..Sequence comparison predicted conserved extracellular DR4 and DR5 O-glycosylation sites; progressive mutation of the DR5 sites attenuated apoptotic signaling...
  67. doi Glycosylation, galectins and cellular signaling
    Cecile Boscher
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada
    Curr Opin Cell Biol 23:383-92. 2011
    b>Glycosylation is a common posttranslational modification of proteins and lipids of the secretory pathway that generates binding sites for galactose-specific lectins or galectins...
  68. doi Recombinant protein production in yeasts
    Diethard Mattanovich
    Department of Biotechnology, University of Natural Resources and Life Sciences, and Austrian Centre of Industrial Biotechnology, Vienna, Austria
    Methods Mol Biol 824:329-58. 2012
    ..In this respect, especially the engineering of yeast glycosylation to produce glycoproteins of human-like glycan structures is of great interest...
  69. pmc SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder
    Vincent Cantagrel
    Neurogenetics Laboratory, Institute for Genomic Medicine, Howard Hughes Medical Institute, Department of Neurosciences and Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 142:203-17. 2010
    N-linked glycosylation is the most frequent modification of secreted and membrane-bound proteins in eukaryotic cells, disruption of which is the basis of the congenital disorders of glycosylation (CDGs)...
  70. pmc Mucin-type O-glycans in human colon and breast cancer: glycodynamics and functions
    Inka Brockhausen
    Department of Medicine and Biochemistry, Human Mobility Research Centre, Queen s University, Kingston General Hospital, Angada 1, Kingston, Ontario K7L 2V7, Canada
    EMBO Rep 7:599-604. 2006
    ..This review focuses on the glycosyltransferases of colon and breast cancer cells that determine the pathways of mucin-type O-glycosylation, and the proposed functional and pathological consequences of altered O-glycans.
  71. pmc Deficiency of Dol-P-Man synthase subunit DPM3 bridges the congenital disorders of glycosylation with the dystroglycanopathies
    Dirk J Lefeber
    Laboratory of Pediatrics and Neurology, Institute for Genetic and Metabolic Disease, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands
    Am J Hum Genet 85:76-86. 2009
    ..Isolated muscular dystrophy has not been described in the congenital disorders of glycosylation (CDG) caused by N-linked protein glycosylation defects...
  72. doi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
    ..Polycomb repression appears to be a critical function of Sxc/Ogt in Drosophila and may be mediated by the glycosylation of Polyhomeotic.
  73. pmc Glycosylation focuses sequence variation in the influenza A virus H1 hemagglutinin globular domain
    Suman R Das
    NIAID, Bethesda, MA, USA
    PLoS Pathog 6:e1001211. 2010
    ..we address an important and largely overlooked issue in antigenic drift: how does the number and location of glycosylation sites affect HA evolution in man? We analyzed the glycosylation status of all full-length H1 subtype HA ..
  74. ncbi Virus glycosylation: role in virulence and immune interactions
    David J Vigerust
    Department of Pediatrics, Program in Vaccine Sciences, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Trends Microbiol 15:211-8. 2007
    The study of N-linked glycosylation as it relates to virus biology has become an area of intense interest in recent years due to its ability to impart various advantages to virus survival and virulence...
  75. pmc Role of N-linked glycans in the functions of hepatitis C virus envelope glycoproteins
    Anne Goffard
    CNRS UPR2511, Institut de Biologie de Lille, France
    J Virol 79:8400-9. 2005
    Hepatitis C virus (HCV) encodes two viral envelope glycoproteins. E1 contains 4 or 5 N-linked glycosylation sites and E2 contains up to 11, with most of the sites being well conserved, suggesting that they play an essential role in some ..
  76. doi Emerging principles for the therapeutic exploitation of glycosylation
    Martin Dalziel
    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Science 343:1235681. 2014
    b>Glycosylation plays a key role in a wide range of biological processes. Specific modification to a glycan's structure can directly modulate its biological function...
  77. doi Mechanisms and principles of N-linked protein glycosylation
    Flavio Schwarz
    Institute of Microbiology, Department of Biology, ETH Zurich, 8093 Zurich, Switzerland
    Curr Opin Struct Biol 21:576-82. 2011
    N-linked glycosylation, a protein modification system present in all domains of life, is characterized by a high structural diversity of N-linked glycans found among different species and by a large number of proteins that are ..
  78. pmc The antifungal drug itraconazole inhibits vascular endothelial growth factor receptor 2 (VEGFR2) glycosylation, trafficking, and signaling in endothelial cells
    Benjamin A Nacev
    Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
    J Biol Chem 286:44045-56. 2011
    ..two previously reported itraconazole activities, failed to recapitulate itraconazole's effects on VEGFR2 glycosylation and signaling. Likewise, glycosylation inhibitors did not alter cholesterol trafficking or inhibit mTOR...
  79. doi Alternative glycosylation modulates function of IgG and other proteins - implications on evolution and disease
    Olga Gornik
    University of Zagreb, Faculty of Pharmacy and Biochemistry, Zagreb, Croatia
    Biochim Biophys Acta 1820:1318-26. 2012
    ..However, contrary to proteins which are defined by their individual genetic templates, glycans are encoded in a complex dynamic network of hundreds of genes which participate in the complex biosynthetic pathway of protein glycosylation.
  80. pmc Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family
    Eric P Bennett
    Department of Odontology, Copenhagen Center for Glycomics, University of Copenhagen, Nørre Allé 20, DK 2200 Copenhagen N, Denmark
    Glycobiology 22:736-56. 2012
    b>Glycosylation of proteins is an essential process in all eukaryotes and a great diversity in types of protein glycosylation exists in animals, plants and microorganisms...
  81. doi N-glycomic biomarkers of biological aging and longevity: a link with inflammaging
    Fabio Dall'olio
    Department of Experimental Pathology, University of Bologna, Via S Giacomo, 14 I 40126 Bologna, Italy
    Ageing Res Rev 12:685-98. 2013
    b>Glycosylation is a frequent co/post-translational modification of proteins which modulates a variety of biological functions. The analysis of N-glycome, i.e...
  82. doi 5-AZA-2'-deoxycytidine induced demethylation influences N-glycosylation of secreted glycoproteins in ovarian cancer
    Radka Saldova
    Dublin Oxford NIBRT Glycobiology Laboratory, NIBRT, Conway Institute, University College Dublin, Belfield, Dublin, Ireland
    Epigenetics 6:1362-72. 2011
    b>Glycosylation is the most common posttranslational modification of proteins and is highly reflective of changes in the environment of a cell...
  83. pmc A unique beta1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, BOKU, University of Natural Resources and Applied Life Sciences, A 1190 Viena, Austria
    Plant Cell 19:2278-92. 2007
    ....
  84. doi Molecular evolution and network-level analysis of the N-glycosylation metabolic pathway across primates
    Ludovica Montanucci
    Department of Experimental and Health Sciences, Institute of Evolutionary Biology, Universitat Pompeu Fabra Consejo Superior de Investigaciones Cientificas, Barcelona, Catalonia, Spain
    Mol Biol Evol 28:813-23. 2011
    N-glycosylation is one of the most important forms of protein modification, serving key biological functions in multicellular organisms...
  85. doi Location, location, location: new insights into O-GalNAc protein glycosylation
    David J Gill
    Institute of Molecular and Cell Biology IMCB, Proteos, 61 Biopolis Drive, Singapore, 138673
    Trends Cell Biol 21:149-58. 2011
    O-GalNAc glycosylation of proteins confers essential structural, protective and signaling roles in eumetazoans...
  86. doi Mining the O-glycoproteome using zinc-finger nuclease-glycoengineered SimpleCell lines
    Catharina Steentoft
    Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Nat Methods 8:977-82. 2011
    ..the O-glycan elongation pathway in human cells, generating stable 'SimpleCell' lines with homogenous O-glycosylation. Three SimpleCell lines expressing only truncated GalNAcα or NeuAcα2-6GalNAcα O-glycans were produced, ..
  87. doi Specific sites of N-linked glycosylation on the hemagglutinin of H1N1 subtype influenza A virus determine sensitivity to inhibitors of the innate immune system and virulence in mice
    Michelle D Tate
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3010, Australia
    J Immunol 187:1884-94. 2011
    ..A reverse genetic approach was used to define the importance of particular N-glycosylation sites on H1 in determining sensitivity to innate immune defenses and virulence in mice...
  88. pmc Engineering mammalian mucin-type O-glycosylation in plants
    Zhang Yang
    Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Flakkebjerg, 4200 Slagelse, Denmark
    J Biol Chem 287:11911-23. 2012
    Mucin-type O-glycosylation is an important post-translational modification that confers a variety of biological properties and functions to proteins...
  89. pmc Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
    Chirag C Sheth
    The Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK
    Med Mycol 49:513-24. 2011
    ..albicans by human PMNs was therefore investigated. N- and O-glycosylation-deficient mutants were attenuated in binding and phagocytosis by PMNs and this was associated with reduced ..
  90. doi Folding of glycoproteins: toward understanding the biophysics of the glycosylation code
    Dalit Shental-Bechor
    Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Curr Opin Struct Biol 19:524-33. 2009
    b>Glycosylation is among the most common post-translational modifications that proteins undergo that may affect many of their activities...
  91. pmc Envelope deglycosylation enhances antigenicity of HIV-1 gp41 epitopes for both broad neutralizing antibodies and their unmutated ancestor antibodies
    Ben Jiang Ma
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, United States of America
    PLoS Pathog 7:e1002200. 2011
    ....
  92. pmc Neisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells
    Michael P Jennings
    Institute for Glycomics, Griffith University, Gold Coast Campus, Australia
    Cell Microbiol 13:885-96. 2011
    ..an O-linked glycan, which may be present as a di- or monosaccharide because of phase variation of select pilin glycosylation genes...
  93. pmc Longer V1V2 region with increased number of potential N-linked glycosylation sites in the HIV-1 envelope glycoprotein protects against HIV-specific neutralizing antibodies
    Marit J van Gils
    Department of Experimental Immunology, Academic Medical Center, M01 120, Meibergdreef 9, 1105 AZ Amsterdam, Netherlands
    J Virol 85:6986-95. 2011
    ..and at a population level, coincided with longer variable loops and an increased number of potential N-linked glycosylation sites (PNGS) in the viral envelope glycoprotein (Env) and, in particular, in variable regions 1 and 2 (V1V2)...
  94. pmc Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
    Mol Cell Proteomics 9:719-27. 2010
    ..In addition, mass spectrometric methodologies to analyze O-glycopeptides were also successful...
  95. pmc Malectin: a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation
    Thomas Schallus
    European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 19:3404-14. 2008
    N-Glycosylation starts in the endoplasmic reticulum (ER) where a 14-sugar glycan composed of three glucoses, nine mannoses, and two N-acetylglucosamines (Glc(3)Man(9)GlcNAc(2)) is transferred to nascent proteins...
  96. doi A novel cerebello-ocular syndrome with abnormal glycosylation due to abnormalities in dolichol metabolism
    Eva Morava
    Radboud University Nijmegen Medical Centre, Institute for Genetic and Metabolic Disease, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Brain 133:3210-20. 2010
    ..and slowly progressive ophthalmological symptoms are common features in patients with congenital disorders of glycosylation type I...
  97. doi Optimal and consistent protein glycosylation in mammalian cell culture
    Patrick Hossler
    Process Sciences Upstream, Technical Operations, Bristol Myers Squibb Company, East Syracuse, NY 13057, USA
    Glycobiology 19:936-49. 2009
    ..b>Glycosylation is a critical protein quality attribute that can modulate the efficacy of a commercial therapeutic ..
  98. ncbi Glycoprotein glycosylation and cancer progression
    J W Dennis
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Ave, Rm 876, M5G 1X5, Toronto, Ont, Canada
    Biochim Biophys Acta 1473:21-34. 1999
    b>Glycosylation of glycoproteins and glycolipids is one of many molecular changes that accompany malignant transformation...
  99. pmc Sialylation and fucosylation of epidermal growth factor receptor suppress its dimerization and activation in lung cancer cells
    Ying Chih Liu
    Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan
    Proc Natl Acad Sci U S A 108:11332-7. 2011
    Protein glycosylation is an important posttranslational process, which regulates protein folding and functional expression. Studies have shown that abnormal glycosylation in tumor cells affects cancer progression and malignancy...
  100. pmc Glycomics profiling of Chinese hamster ovary cell glycosylation mutants reveals N-glycans of a novel size and complexity
    Simon J North
    Division of Molecular Biosciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom
    J Biol Chem 285:5759-75. 2010
    ..glycans and the pathways by which they are synthesized has been greatly facilitated by investigations of glycosylation mutants of cultured cell lines and model organisms...
  101. pmc In planta protein sialylation through overexpression of the respective mammalian pathway
    Alexandra Castilho
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Biol Chem 285:15923-30. 2010
    ..Sialylation was at great uniformity when glycosylation mutants that lack plant-specific N-glycan residues were used as expression hosts...

Research Grants78

  1. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2013
    ..We find that metabolite availability to the Golgi N- glycosylation pathway exerts autonomous control over the assembly of macromolecular complexes on the cell surface, and in ..
  2. C5a as an Anti-HIV Microbicidal Candidate
    PHILIPPE ANDRE GALLAY; Fiscal Year: 2013
    ..Remarkably, we found that acetylation, amidation and glycosylation of C5A greatly enhanced C5A anti-HIV activities in genital fluids...
  3. Novel Bioconjugate Vaccines to Prevent Staphylococcus aureus Infection
    JEAN CLAIRE LEE; Fiscal Year: 2013
    ..This protein glycosylation system has been functionally transferred into Escherichia coli, and it allows the conjugation of an antigenic ..
  4. Use of Phosphodiesterase Inhibitors to Evaluate the Pathobiology of CF
    JENNIFER L TAYLOR-COUSAR; Fiscal Year: 2013
    ..results in normalization of organellar hyperacidification and its downstream effects, including disordered glycosylation, predisposition to adherence by Pseudomonas aeruginosa and consequent inflammation...
  5. Phospholipid flip-flop in biogenic membranes
    Anant K Menon; Fiscal Year: 2013
    ..Lipid flip-flop across the endoplasmic reticulum (ER) membrane is required for protein N-glycosylation and GPI-anchoring...
  6. Misregulation of receptor tyrosine kinase signaling in PKD
    Jordan A Kreidberg; Fiscal Year: 2013
    ..Failure to ubquitinate c-Met is due to sequestration of the E3 ubiquitin ligase c-Cbl by 31 integrin in the Golgi apparatus. This grant will investigate the roles of c-Cbl, c-Met and protein glycosylation in the pathogenesis of PKD.
  7. Lactoferrin for Immunomodulation of Systemic Inflammatory Response Syndrome
    Marian L Kruzel; Fiscal Year: 2011
    ..inflammatory response syndrome (SIRS) using a novel human recombinant lactoferrin that contains "humanized" glycosylation patterns...
  8. Antigen receptor inputs: linking structural, molecular, and cellular responses
    Jonathan P Schneck; Fiscal Year: 2012
    ..We will use an shRNA screen to focus on pathways known to affect T cell receptor clustering -glycosylation and the role of cholesterol and its interaction with the cytoskeleton...
  9. Mechanistic Studies of HCV E2
    Joseph Marcotrigiano; Fiscal Year: 2013
    ..make sufficient quantities of properly folded protein as E2 protein production is challenging due to its heavy glycosylation and presence of intramolecular disulfide bonds...
  10. N-Glycosylation And ER Stress
    MARK LEHRMAN; Fiscal Year: 2012
    ..First, there are 13 human genetic diseases in the family "Congenital Disorders of Glycosylation" (CDG) Type I, with defective LLO synthesis...
  11. Photocleavable Bead Technology for Glycomics
    Mark Lim; Fiscal Year: 2013
    ..effective techniques that can detect and analyze PTMs which can be highly complex, especially in the case of glycosylation of proteins...
  12. IMMUNOLOGY OF UNIQUE TUMOR SPECIFIC ANTIGENS
    Hans Schreiber; Fiscal Year: 2013
    ..g., colon, breast, lung, prostate, ovary and pancreas) have defects in O-linked glycosylation thereby generating tumor-specific epitopes on surface glycoproteins of cancer cells...
  13. Biology of the HMW1 and HMW2 Adhesins of H. Influenzae
    JOSEPH W ST. GEME; Fiscal Year: 2013
    ..In additional experiments, we will elucidate the molecular details of glycosylation, defining the relationship between specific asparagine sites of glycosylation and the adhesive function of ..
  14. Analysis of PTH and Dopamine Receptor Signaling in Proximal Tubules
    Eleanor D Lederer; Fiscal Year: 2013
    ..Npt2a translated in the NHERF-deficient cells (OK-H) lacked a critical post-translational modification, glycosylation. These proteins, instead of trafficking to apical membrane, accumulated in a perinuclear location...
  15. Keratin Biology and Disease Aspects in Simple Epithelia
    Bishr Omary; Fiscal Year: 2013
    ..to use two approaches: (a) a targeted approach to understand specific aspects of keratin regulation;namely glycosylation, keratin associated proteins (KAPs), and keratin cleavage by caspases, and (b) an unbiased approach to explore ..
  16. Regulation of IGF-1 and Insulin Signaling by O-GlcNAc Glycosylation
    LAUREN ELIZABETH BALL; Fiscal Year: 2013
    ..IRS-1 and IRS-2 are also glycosylated at Ser/Thr residues by N-acetylglucosamine (O-GlcNAc). This unique glycosylation is a reversible posttranslational modification proposed to regulate protein function in a manner analogous to ..
  17. Research and Development of a Novel System to Produce Polysaccharide Conjugate Va
    Peng George Wang; Fiscal Year: 2013
    ..The discovery and further development of bacterial protein N- glycosylation system have provided a novel approach to solve this biomedical problem...
  18. HA Surface Presented Yeast Flu Vaccine and Its Enhancement by CD154 Codisplay
    Kaiming Ye; Fiscal Year: 2012
    ..Although yeast has different glycosylation capability as compared to mammalian cells, this limited glycosylation may actually be a benefit to formulating ..
  19. P63/IGFBP3/BCL2 Control of Corneal Epithelial Homeostasis
    Danielle M Robertson; Fiscal Year: 2011
    ..quantum dots using time-lapse confocal microscopy);and characterize the role of dimerization and glycosylation in subcellular trafficking and apoptosis (BiFC, site-directed mutagenesis, cell fractionation, WB, and Annexin ..
  20. Fine-Tuning the Notch Signaling Pathway via O-Glucosylation
    Hamed Jafar-Nejad; Fiscal Year: 2013
    ..of the rumi phenotype, to test the hypothesis that Rumi regulates Notch signaling via altering the glycosylation pattern of the Notch protein in vivo, and to identify other Notch "fine-tuning" genes by screening for genetic ..
  21. GLYCOSYLATION MUTANTS OF LEISHMANIA
    Salvatore J Turco; Fiscal Year: 2012
    ..Our ultimate goal is a comprehensive understanding of the genes and gene products responsible for synthesizing Leishmania surface and secreted molecules, and their individual and specific roles in parasite virulence. ..
  22. In vivo phenotypic effects of naturally occuring sequence variations in WNV
    Robin M Moudy; Fiscal Year: 2010
    ..one of the domains required for efficient fusion, it is located three amino acids downstream from an N-linked glycosylation site present in the North American strains of WNV and may therefore affect its glycosylation...
  23. Lipid Translocating Glycosyltransferases in Congenital Neurological Disease
    PETER AB ORLEAN; Fiscal Year: 2010
    ..Congenital defects in asparagine-linked glycosylation, protein O-mannosylation, and glycosylphosphatidylinositol membrane anchoring of protein cause abnormal ..
  24. GLYCOSYLTRANSFERASE FUNCTION IN DEVELOPMENT AND DISEASE
    JAMEY MARTH; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Glycosylation of cellular proteins is linked to an increasing number of important ontogenic and pathogenic mechanisms...
  25. The role of BNST CGRP in stress-enhanced anxiety behavior and HPA-axis function
    Kelly S Sink; Fiscal Year: 2013
    ..of RAMP1, the CGRP receptor subunit that confers pharmacological specificity for CGRP and influences glycosylation and trafficking of the CGRP receptor to the cell surface...
  26. Role of mucin-type O-glycosylation in Trypanosoma cruzi biology
    Christopher M West; Fiscal Year: 2012
    ..Our goal is to determine if mucin-type O-glycosylation works as virulence factor and help the parasite to survive and succeed in its different environments during ..
  27. Systems Biology of Glycosylation
    Sriram Neelamegham; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Glycosylation is an important post-translational modification of proteins and lipids...
  28. Defining the interaction of FCRLA with immunoglobulin
    PETER DANIEL BURROWS; Fiscal Year: 2013
    ..It lacks N-linked glycosylation sites and a transmembrane region, but contains a signal sequence to target it to the ER...
  29. Glycosyltransferase Golgi Retention Mechanism
    Pi Wan Cheng; Fiscal Year: 2013
    ..and C2GnT-L in controlling intracellular trafficking and Golgi localization of these two enzymes, and mucin glycosylation. A549 and H292 cells with and without heat shock will be the test models because they express C2GnT-L, C2GnT-M,..
  30. Evaluation of the Effects of Chikungunya N-linked Glycosylation on Pathogenesis
    CHARLES EDWIN MCGEE; Fiscal Year: 2013
    ....
  31. Protective immunity against HIV: the role of IgG subclass and glycosylation
    Donald Forthal; Fiscal Year: 2009
    ..We will investigate two biological properties of antibody, IgG subclass and Fc glycosylation, that impact its ability to bind to Fc?Rs and consequently to inhibit HIV-1...
  32. Cytosolic O-Glycosylation and Insulin Signaling
    Gerald Warren Hart; Fiscal Year: 2013
    ....
  33. N-acetylglucosamine signaling in fungi
    James B Konopka; Fiscal Year: 2012
    ..This amino sugar is a key component of GPI anchors and N-linked glycosylation on proteins, and is a core constituent of extracellular polymers, such as keratin or hyaluronan in humans and ..
  34. The Roles of Sodium Pump Beta Subunits in Mammalian Cells
    Jack H Kaplan; Fiscal Year: 2010
    ..Throughout these experiments we will also probe the physiological role of p subunit glycosylation, which is currently unknown...
  35. Mechanism of Heat Shock Protein Induction by Glutamine
    Paul E Wischmeyer; Fiscal Year: 2012
    ..We hypothesize GLN induces HSP expression via activation of the O-linked glycosylation pathway (O-GlcNAc), which is known to depend on GLN as a rate limiting substrate...
  36. Bifunctional antibodies with targeted CNS delivery against West Nile virus
    Qiang Chen; Fiscal Year: 2013
    ..developed a plant-derived humanized MAb with promising therapeutic potential, with a desired human N-linked glycosylation pattern...
  37. Synthetic saccharides to study immunological properties of Streptococcus pneumoni
    Alexei V Demchenko; Fiscal Year: 2010
    ..Supported by promising preliminary results, the application of a new thioimidate glycosylation method in the synthesis of glycostructures to study immunological properties of SPn6A is proposed...
  38. Metabolic Engineering of Chinese Hamster Ovary Cells for Production of Heparin
    Susan T Sharfstein; Fiscal Year: 2011
    ..By engineering the CHO cell glycosylation pathway, which normally affords the related polysaccharide heparan sulfate, a metabolically engineered heparin ..
  39. Multiplex Fluorescent Zdyes for Differential Glycomic Detection
    EDWARD DRATZ; Fiscal Year: 2009
    Protein posttranslational modifications (PTMs) control much of biology. Glycosylation is one of the most common PTMs and more powerful tools are needed to study the occurrence and relative levels of glycosylation...
  40. Defining the epitope in antiNMDA receptor encephalitis
    DAVID ROBINSON LYNCH; Fiscal Year: 2010
    ..Furthermore, creation of the epitope is blocked by tunicamycin and disruption of a specific N- linked glycosylation/deamidation site in NR1 removes immunoreactivity, suggesting that site specific N-linked glycosylation and ..
  41. Lectin Resistant Tumor Cells And Functional Glycomics
    PAMELA M STANLEY; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): The long term goal of this program is to identify new factors that affect glycosylation, and to determine how they impact the functional mammalian glycome...
  42. Lubricin Function in Articulating Joints
    Matthew L Warman; Fiscal Year: 2012
    ..the expression of different protein splice-forms, and specific post-translational processing events, such as glycosylation and protein cleavage, which appear to be important to the protein's biological and mechanical activities...
  43. Testing Substrate-Flux Therapies for Glycosylation Disorders using Zebrafish
    Richard Steet; Fiscal Year: 2010
    ..a novel therapeutic strategy for a group of rare inherited metabolic disorders, the Congenital Disorders of Glycosylation-Type I (CDG-I)...
  44. The Role of Lipid Rafts in Vpu Function
    EDWARD BRICE STEPHENS; Fiscal Year: 2011
    ..region followed by a transmembrane domain, a central extracellular coiled-coiled domain containing two N-liked glycosylation sites and a C-terminal glycosyl-phosphatidylinositol (GPI) anchor...
  45. STRUCTURAL BASIS OF ORGANELLE TETHERING
    Adam D Linstedt; Fiscal Year: 2013
    ..products in which these proteins depend on secretory processes such as protein folding, quality control, glycosylation, proteolytic activation, and localization...
  46. Structure and Function of the B. anthracis Exosporium
    Charles L Turnbough; Fiscal Year: 2010
    ..generally important mechanisms of protein-protein interactions, macromolecular assembly, protein attachment to cell surfaces, glycosylation and phosphorylation of bacterial proteins, and host-pathogen interactions.
  47. Novel Aspects of the Cardiac Renin-Angiotensin System
    KENNETH MELVIN BAKER; Fiscal Year: 2012
    ..to identify hyperglycemia-induced cellular events, such as the hexosamine biosynthesis pathway, protein O-glycosylation and oxidative stress, on regulation of the intracellular RAS, by pharmacological and genetic approaches...
  48. Human Autoimmunity and Genetic Defects in N-Glycosylation
    Michael Demetriou; Fiscal Year: 2013
    ..We find that metabolite availability to the Golgi N- glycosylation pathway exerts autonomous control over the assembly of macromolecular complexes on the cell surface, and in ..
  49. GENETIC ANALYSIS OF SYNAPSE FORMATION AND FUNCTION
    Kendal Broadie; Fiscal Year: 2013
    ..the last five years provide the foundation of this proposal, by highlighting the importance of extracellular glycosylation and proteolytic mechanisms shaping trans-synaptic signaling...
  50. Genesis of Epithelial Cell Lumens
    Jennifer L Harder; Fiscal Year: 2013
    ..Her immediate goals are designed to facilitate her successful transition to independence: 1) learn to analyze glycosylation modifications of proteins which she will apply to her study of mechanisms controlling polarity, 2) (which will ..
  51. Biosignature of the endometrium in women with endometriosis
    Neil Sidell; Fiscal Year: 2010
    ..this grant application, we will profile the protein expression patterns, tyrosine phosphorylation status, and glycosylation patterns of 247 biomarkers in 50 patients'and 50 control biopsy tissues...
  52. Functional Definition of MYC Acetylation
    MATTHEW THOMAS HURD; Fiscal Year: 2013
    ..Until recently phosphorylation and O-glycosylation of a few residues were the only PTMs reported, which affect MYC ubiqitination and degradation by the ..
  53. Biogenic Amine Transporters: Mechanisms of Ligand Interaction
    MAARTEN E REITH; Fiscal Year: 2013
    ..There is evidence that oligomerization and glycosylation of biogenic amine transporters are intimately linked, but there is contrasting evidence as to which comes ..
  54. MOLECULAR STUDIES OF RETINAL DEGENERATION IN DROSOPHILA
    Nansi J Colley; Fiscal Year: 2013
    ..those events during protein biosynthesis in the secretory pathway that ensure correct protein translocation, glycosylation, folding, oligomeric assembly, quality control, transport and targeting...
  55. Control of Mucin Glycan Branching in Membrane-bound and Secreted Mucins
    Pi Wan Cheng; Fiscal Year: 2010
    ..develop strategies to design therapeutics to treat patients with lung diseases associated with altered mucin glycosylation. PUBLIC HEALTH RELEVANCE: The work proposed could advance our fundamental understanding of the key factors ..
  56. Bacteria-Mucin Interactions at the Ocular Surface
    Ilene K Gipson; Fiscal Year: 2012
    ..Our Specific Aims are: Aim I: Compare ectodomain release, cell surface density and glycosylation characteristics of MAMs MUC1, MUC4 and MUC16 on human corneal and conjunctival epithelial cells, in response ..
  57. Minor fimbriae of P. gingivalis and dendritic cells
    AMIR EMANUEL ZEITUNI; Fiscal Year: 2010
    ..gingivalis by monocyte derived dendritic cells (MoDCs). Aim 2: To purify the minor fimbriae and identify glycosylation motifs. Aim 3. To determine the role of minor fimbriae of P...
  58. O-linked glycosylation and synaptic function in hippocampus
    LORI LYNN contact MCMAHON; Fiscal Year: 2010
    ..glucose levels are in excess, which leading to a pathologically increase amount of proteins with an O-linked glycosylation tag...
  59. Role of the TRPV1 channel in myocardial salvage from ischemia-reperfusion injury
    ERIC RICHARD GROSS; Fiscal Year: 2012
    ..I will determine the molecular basis by studying whether TRPV1 glycosylation (Aim 1) and secondly, protein kinase C epsilon (PKC5) phosphorylation (Aim2), reduce heart cell injury...
  60. The accessory Sec system of Gram-positive pathogens
    Paul M Sullam; Fiscal Year: 2013
    ..e., the role of this protein in mediating both GspB export and glycosylation. The impact of Asp2 binding on GspB export will be addressed through a series of targeted mutations...
  61. HIV Envelope-based vaccines from superinfection to elicit neutralizing antibodies
    ANN JONES HESSELL; Fiscal Year: 2013
    ..Potential N-linked glycosylation sites will be examined and other mutations that are known to cause resistance to several broadly neutralizing ..
  62. N-Linked Protein Glycosylation: Pathways and Processes
    Barbara Imperiali; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Asparagine-linked protein glycosylation is involved in a wide range of biological processes that are implicated in human health and disease...
  63. The Aging Pituitary-Gonadal Axis
    George R Bousfield; Fiscal Year: 2013
    ..A double hFSH glycosylation mutant will replace the normal mFSH gene...
  64. A novel transgenic silkworm system for recombinant glycoprotein production
    Donald L Jarvis; Fiscal Year: 2010
    ..The major impediment is that the endogenous protein glycosylation pathways of the silk gland cannot be expected to properly glycosylate higher eukaryotic glycoproteins...
  65. A SARS-CoV Spike Protein Vaccine
    B Sim; Fiscal Year: 2005
    ..Region I was selected in part because of paucity of N-glycosylation sites. However, there are 9 such sites in the native sequence...
  66. Research and Development of a Novel System to Produce Polysaccharide Conjugate Va
    Peng Wang; Fiscal Year: 2009
    ..The objective of this application is to explore a recently established bacterial protein O-glycosylation system to obtain polysaccharide conjugate vaccines in a facile, efficient, and easily applicable manner...
  67. Glycosylation of full-length antibodies in Escherichia coli
    ADAM FISHER; Fiscal Year: 2009
    ..Since monoclonal antibodies require glycosylation to provide full therapeutic benefit, mammalian cell culture is the expression platform of choice...
  68. Humanizing N-linked glycosylation in Escherichia coli
    ADAM FISHER; Fiscal Year: 2009
    ..One of the most important of these modifications, N-linked protein glycosylation, is predicted to affect more than half of all eukaryotic protein species and is often essential for proper ..
  69. Molecular basis of congenital disorders of glycosylation: mechanisms of oligosach
    Smita Mohanty; Fiscal Year: 2010
    ..defects in this modification pathway in humans cause a class of disorders known as congenital disorders of glycosylation (CDG)...
  70. Profiling Urine Glycosylation of PSA and other Glyco-Biomarkers in Prostate Cance
    Lewis Pannell; Fiscal Year: 2009
    ..Both PSA and PAP are glycosylated and changes in glycosylation go hand-in-hand with cancer...
  71. GLYCOSYLATION MECHANISMS
    CARLOS HIRSCHBERG; Fiscal Year: 2000
    ..of this research proposal are the following: (a) To understand the cellular mechanisms which regulate the glycosylation of proteins and lipids in the rough endoplasmic reticulum and Golgi apparatus and, (b) to establish the role ..
  72. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2003
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  73. Model Vaccines Exploiting Fungal Mannosylation
    STUART LEVITZ; Fiscal Year: 2009
    ..Cryptococcal MP contain serine/threonine (S/T)-rich regions which serve as sites for extensive O-linked glycosylation as well as N-X-S/T sequons that serve as N-glycosylation sites...
  74. Probing the Glycan Biosynthetic Machinery of Campylobacter Jejuni.
    JERRY TROUTMAN; Fiscal Year: 2009
    N-linked protein glycosylation, the formation of a glycosylamide bond between an oligosaccharide and an asparagine side chain, is an essential protein modification required for the function, folding and routing of cell surface receptors,..
  75. Chemical tools to understand and target Helicobacter pylori glycosylation
    Danielle H Dube; Fiscal Year: 2010
    ..Although Hp's glycans and glycosylation machinery are linked to pathogenesis, Hp's glycome remains poorly understood...
  76. IgA-secreting B cell lines: a novel tool for studies of IgA nephropathy
    Jan Novak; Fiscal Year: 2009
    ..Several lines of evidence suggest a direct causal relationship between aberrant glycosylation, the formation of immune complexes containing aberrantly glycosylated IgA1, their deposition in the mesangium, ..
  77. MOLECULAR ANALYSIS OF MUCIN TYPE O GLYCOSYLATION
    Michael Hollingsworth; Fiscal Year: 2000
    ..GaINAc) by an O-glycosidic linkage to serine and threonine residues (the first step in mucin-type glycosylation) show distinct specificities for some substrates and common specificities for other substrates (albeit with ..
  78. Molecular biology of TPPI/CLN2 protein
    Adam Golabek; Fiscal Year: 2006
    ..We propose to further elucidate the role of N-glycosylation in the trafficking of TPP I as well as to determine whether and how N-glycosylation affects folding, activity, ..