glycosylation

Summary

Summary: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.

Top Publications

  1. pmc O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding
    Takako Yoshida-Moriguchi
    Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 327:88-92. 2010
  2. ncbi Antibody neutralization and escape by HIV-1
    Xiping Wei
    Howard Hughes Medical Institute, University of Alabama at Birmingham, 720 South 20th Street, Kaul 816, Birmingham, Alabama 35294 0024, USA
    Nature 422:307-12. 2003
  3. ncbi Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites
    Karin Julenius
    Center for Biological Sequence Analysis, BioCentrum, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Glycobiology 15:153-64. 2005
  4. pmc Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
    A Trkola
    Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA
    J Virol 70:1100-8. 1996
  5. pmc Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgammaRIII and antibodies lacking core fucose
    Claudia Ferrara
    Pharma Research and Early Development, Roche Glycart AG, CH 8952 Schlieren, Switzerland
    Proc Natl Acad Sci U S A 108:12669-74. 2011
  6. pmc A multifunctional mannosyltransferase family in Candida albicans determines cell wall mannan structure and host-fungus interactions
    Hector M Mora-Montes
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, UK
    J Biol Chem 285:12087-95. 2010
  7. doi Dystroglycanopathies: coming into focus
    Caroline Godfrey
    Dubowitz Neuromuscular Centre, UCL Institute of Child Health and the Great Ormond Street Hospital for Children, Guilford Street, University College London, United Kingdom
    Curr Opin Genet Dev 21:278-85. 2011
  8. pmc SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder
    Vincent Cantagrel
    Neurogenetics Laboratory, Institute for Genomic Medicine, Howard Hughes Medical Institute, Department of Neurosciences and Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 142:203-17. 2010
  9. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
  10. ncbi Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence
    Nikolaj Blom
    Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark
    Proteomics 4:1633-49. 2004

Detail Information

Publications352 found, 100 shown here

  1. pmc O-mannosyl phosphorylation of alpha-dystroglycan is required for laminin binding
    Takako Yoshida-Moriguchi
    Howard Hughes Medical Institute, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 327:88-92. 2010
    ..These findings expand our understanding of the mechanisms that underlie congenital muscular dystrophy...
  2. ncbi Antibody neutralization and escape by HIV-1
    Xiping Wei
    Howard Hughes Medical Institute, University of Alabama at Birmingham, 720 South 20th Street, Kaul 816, Birmingham, Alabama 35294 0024, USA
    Nature 422:307-12. 2003
    ..sparse, did not map generally to known neutralization epitopes, and involved primarily changes in N-linked glycosylation. This pattern of escape, and the exceptional density of HIV-1 envelope glycosylation generally, led us to ..
  3. ncbi Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites
    Karin Julenius
    Center for Biological Sequence Analysis, BioCentrum, Building 208, Technical University of Denmark, DK 2800 Lyngby, Denmark
    Glycobiology 15:153-64. 2005
    O-GalNAc-glycosylation is one of the main types of glycosylation in mammalian cells...
  4. pmc Human monoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1
    A Trkola
    Aaron Diamond AIDS Research Center, New York University School of Medicine, New York 10016, USA
    J Virol 70:1100-8. 1996
    ..consistent with this, antibodies able to block 2G12 binding to recombinant gp120 were not detected in significant quantities in 16 HIV-positive human serum samples...
  5. pmc Unique carbohydrate-carbohydrate interactions are required for high affinity binding between FcgammaRIII and antibodies lacking core fucose
    Claudia Ferrara
    Pharma Research and Early Development, Roche Glycart AG, CH 8952 Schlieren, Switzerland
    Proc Natl Acad Sci U S A 108:12669-74. 2011
    ..These findings allow us to understand the higher efficacy of therapeutic antibodies lacking the core fucose and also suggest a unique mechanism by which the immune system can regulate antibody-mediated effector functions...
  6. pmc A multifunctional mannosyltransferase family in Candida albicans determines cell wall mannan structure and host-fungus interactions
    Hector M Mora-Montes
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, Scotland, UK
    J Biol Chem 285:12087-95. 2010
    ..b>Glycosylation of cell wall proteins involves the activities of families of endoplasmic reticulum and Golgi-located glycosyl ..
  7. doi Dystroglycanopathies: coming into focus
    Caroline Godfrey
    Dubowitz Neuromuscular Centre, UCL Institute of Child Health and the Great Ormond Street Hospital for Children, Guilford Street, University College London, United Kingdom
    Curr Opin Genet Dev 21:278-85. 2011
    A common group of muscular dystrophies is associated with the aberrant glycosylation of α-dystroglycan...
  8. pmc SRD5A3 is required for converting polyprenol to dolichol and is mutated in a congenital glycosylation disorder
    Vincent Cantagrel
    Neurogenetics Laboratory, Institute for Genomic Medicine, Howard Hughes Medical Institute, Department of Neurosciences and Pediatrics, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 142:203-17. 2010
    N-linked glycosylation is the most frequent modification of secreted and membrane-bound proteins in eukaryotic cells, disruption of which is the basis of the congenital disorders of glycosylation (CDGs)...
  9. ncbi Intracellular functions of N-linked glycans
    A Helenius
    Institute of Biochemistry, , , , Switzerland
    Science 291:2364-9. 2001
    ..The division of synthesis and processing between the ER and the Golgi complex represents an evolutionary adaptation that allows efficient exploitation of the potential of oligosaccharides...
  10. ncbi Prediction of post-translational glycosylation and phosphorylation of proteins from the amino acid sequence
    Nikolaj Blom
    Center for Biological Sequence Analysis, The Technical University of Denmark, Lyngby, Denmark
    Proteomics 4:1633-49. 2004
    ..We describe our experiences with the development of prediction methods for phosphorylation and glycosylation sites and the development of PTM-specific databases...
  11. pmc Dystroglycan function requires xylosyl- and glucuronyltransferase activities of LARGE
    Kei Ichiro Inamori
    Howard Hughes Medical Institute, Department of Molecular Physiology and Biophysics, University of Iowa Roy J and Lucille A Carver College of Medicine, 4283 Carver Biomedical Research Building, 285 Newton Road, Iowa City, IA 52242 1101, USA
    Science 335:93-6. 2012
    ..This modification allowed α-DG to bind laminin-G domain-containing ECM ligands...
  12. ncbi Envelope-constrained neutralization-sensitive HIV-1 after heterosexual transmission
    Cynthia A Derdeyn
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL 35294 USA
    Science 303:2019-22. 2004
    ..This reveals characteristics of the envelope glycoprotein that influence HIV-1 transmission and may have implications for vaccine design...
  13. doi Mechanisms and principles of N-linked protein glycosylation
    Flavio Schwarz
    Institute of Microbiology, Department of Biology, ETH Zurich, 8093 Zurich, Switzerland
    Curr Opin Struct Biol 21:576-82. 2011
    N-linked glycosylation, a protein modification system present in all domains of life, is characterized by a high structural diversity of N-linked glycans found among different species and by a large number of proteins that are ..
  14. doi Sweet-talk: role of host glycosylation in bacterial pathogenesis of the gastrointestinal tract
    A P Moran
    Microbiology, School of Natural Sciences, National University of Ireland, Galway, Ireland
    Gut 60:1412-25. 2011
    b>Glycosylation is a key modification of proteins and lipids and is involved in most intermolecular and intercellular interactions...
  15. doi Precision mapping of an in vivo N-glycoproteome reveals rigid topological and sequence constraints
    Dorota F Zielinska
    Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Am Klopferspitz 18, Martinsried D 82152, Germany
    Cell 141:897-907. 2010
    N-linked glycosylation is a biologically important protein modification, but only a small fraction of modification sites have been mapped...
  16. doi Protein glycosylation in bacteria: sweeter than ever
    Harald Nothaft
    Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    Nat Rev Microbiol 8:765-78. 2010
    Investigations into bacterial protein glycosylation continue to progress rapidly...
  17. doi Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    Plant Biotechnol J 6:392-402. 2008
    ..using plants as a production system for therapeutic proteins is their inability to perform authentic human N-glycosylation (i.e. the presence of beta1,2-xylosylation and core alpha1,3-fucosylation)...
  18. pmc Neutralizing antibody responses in acute human immunodeficiency virus type 1 subtype C infection
    E S Gray
    AIDS Virus Research Unit, National Institute for Communicable Diseases, Private Bag X4, Sandringham 2131, Johannesburg, South Africa
    J Virol 81:6187-96. 2007
    ..The magnitude of this response was associated with shorter V1-to-V5 envelope lengths and fewer glycosylation sites, particularly in the V1-V2 region...
  19. doi Glycosylation, galectins and cellular signaling
    Cecile Boscher
    Department of Cellular and Physiological Sciences, Life Sciences Institute, University of British Columbia, Vancouver, BC, Canada
    Curr Opin Cell Biol 23:383-92. 2011
    b>Glycosylation is a common posttranslational modification of proteins and lipids of the secretory pathway that generates binding sites for galactose-specific lectins or galectins...
  20. ncbi Anti-inflammatory activity of immunoglobulin G resulting from Fc sialylation
    Yoshikatsu Kaneko
    Laboratory of Molecular Genetics and Immunology, Rockefeller University, 1230 York Avenue, New York, NY 10021, USA
    Science 313:670-3. 2006
    ..This differential sialylation may provide a switch from innate anti-inflammatory activity in the steady state to generating adaptive pro-inflammatory effects upon antigenic challenge...
  21. pmc The expanding horizons of asparagine-linked glycosylation
    Angelyn Larkin
    Department of Chemistry Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA
    Biochemistry 50:4411-26. 2011
    Asparagine-linked glycosylation involves the sequential assembly of an oligosaccharide onto a polyisoprenyl donor, followed by the en bloc transfer of the glycan to particular asparagine residues within acceptor proteins...
  22. pmc Candida albicans cell wall glycosylation may be indirectly required for activation of epithelial cell proinflammatory responses
    Celia Murciano
    Department of Oral Immunology, Floor 28, Tower Wing, King s College London Dental Institute, King s College London, London SE1 9RT, United Kingdom
    Infect Immun 79:4902-11. 2011
    ..host recognition and immune activation in myeloid cells, we sought to determine whether β-glucan and N- or O-glycosylation was important in activating the MAPK/MKP1/c-Fos hypha-mediated response mechanism and proinflammatory ..
  23. pmc Control of mucin-type O-glycosylation: a classification of the polypeptide GalNAc-transferase gene family
    Eric P Bennett
    Department of Odontology, Copenhagen Center for Glycomics, University of Copenhagen, Nørre Allé 20, DK 2200 Copenhagen N, Denmark
    Glycobiology 22:736-56. 2012
    b>Glycosylation of proteins is an essential process in all eukaryotes and a great diversity in types of protein glycosylation exists in animals, plants and microorganisms...
  24. ncbi Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans
    Steven Bates
    School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, United Kingdom
    J Biol Chem 281:90-8. 2006
    ..However, tissue burdens for the Caoch1delta null mutant were similar to control strains with normal N-glycosylation, suggesting the host-fungal interaction was altered such that high burdens were tolerated...
  25. doi Pandemic H1N1 influenza A viruses are resistant to the antiviral activities of innate immune proteins of the collectin and pentraxin superfamilies
    Emma R Job
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
    J Immunol 185:4284-91. 2010
    ..of H1N1 viruses to infect human respiratory epithelial cells in a manner that correlated with the degree of glycosylation in the globular head of the hemagglutinin...
  26. ncbi Statistical analysis of the protein environment of N-glycosylation sites: implications for occupancy, structure, and folding
    Andrei J Petrescu
    Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford OX1 3QU, U K
    Glycobiology 14:103-14. 2004
    ..analysis to the glycan-protein linkage, and the peptide primary, secondary, and tertiary structures around N-glycosylation sites...
  27. pmc Characterization of the PMT gene family in Cryptococcus neoformans
    Sven D Willger
    Institut fur Mikrobiologie, Molekulare Mykologie, Heinrich Heine Universitat, Dusseldorf, Germany
    PLoS ONE 4:e6321. 2009
    Protein-O-mannosyltransferases (Pmt's) catalyze the initial step of protein-O-glycosylation, the addition of mannose residues to serine or threonine residues of target proteins.
  28. pmc Aberrant glycosylation of IgA1 is inherited in both pediatric IgA nephropathy and Henoch-Schönlein purpura nephritis
    Krzysztof Kiryluk
    Division of Nephrology, Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, New York 10032, USA
    Kidney Int 80:79-87. 2011
    ..Thus, serum galactose-deficient IgA1 levels are highly inherited in pediatric patients with IgAN and HSPN, providing support for another shared pathogenic link between these disorders...
  29. ncbi Mutations in the human LARGE gene cause MDC1D, a novel form of congenital muscular dystrophy with severe mental retardation and abnormal glycosylation of alpha-dystroglycan
    Cheryl Longman
    Dubowitz Neuromuscular Centre, Imperial College, Hammersmith Campus, London, UK
    Hum Mol Genet 12:2853-61. 2003
    ..This is the first description of mutations in the human LARGE gene and we propose to name this new disorder MDC1D...
  30. pmc Glycosylation in immune cell trafficking
    Markus Sperandio
    Walter Brendel Center of Experimental Medicine, Ludwig Maximilians Universitat, Munich, Germany
    Immunol Rev 230:97-113. 2009
    ..Advancing the knowledge on glycan function into appropriate pathology models is likely to suggest interesting new therapeutic strategies in the treatment of immune- and inflammation-mediated diseases...
  31. pmc Inhibition of Lassa and Marburg virus production by tetherin
    Toshie Sakuma
    First Department of Forensic Science, National Research Institute of Police Science, Kashiwa 277 0882, Japan
    J Virol 83:2382-5. 2009
    ..by viral matrix proteins of Lassa virus or Marburg virus was markedly inhibited by tetherin and that N-linked glycosylation of tetherin was dispensable for this antiviral activity...
  32. pmc Pattern recognition receptors require N-glycosylation to mediate plant immunity
    Heidrun Häweker
    Max Planck Institute for Plant Breeding Research, Carl von Linne Weg 10, 50829 Cologne, Germany
    J Biol Chem 285:4629-36. 2010
    ..and FLS2, sensing bacterial translation elongation factor Tu (elf18) and flagellin (flg22), respectively, in N-glycosylation mutants...
  33. pmc Envelope glycans of immunodeficiency virions are almost entirely oligomannose antigens
    Katie J Doores
    Department of Immunology and Microbial Science and International AIDS Vaccine Initiative Neutralizing Antibody Center, The Scripps Research Institute, La Jolla, CA 92037, USA
    Proc Natl Acad Sci U S A 107:13800-5. 2010
    ..In stark contrast to recombinant gp120, which shows extensive exposure to cellular glycosylation enzymes (>70% complex type glycans), the native envelope shows barely detectable processing beyond the ..
  34. ncbi HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites
    Peter D Kwong
    Vaccine Research Center, National Institutes of Health, Bethesda, Maryland 20892, USA
    Nature 420:678-82. 2002
    ..Because this solution is available for cell-surface receptors but not for most antibodies, conformational masking enables HIV-1 to maintain receptor binding and simultaneously to resist neutralization...
  35. pmc Glycosylation defining cancer malignancy: new wine in an old bottle
    Senitiroh Hakomori
    Pacific Northwest Research Institute, and Department of Pathobiology, University of Washington, Seattle, WA 98122 4327, USA
    Proc Natl Acad Sci U S A 99:10231-3. 2002
  36. pmc Human immunodeficiency virus type 1 V1-V2 envelope loop sequences expand and add glycosylation sites over the course of infection, and these modifications affect antibody neutralization sensitivity
    Manish Sagar
    Department of Medicine, Brigham and Women s Hospital, Cambridge, Massachusetts 02139, USA
    J Virol 80:9586-98. 2006
    ..Changes in the envelope variable loop sequences, particularly the extent of glycosylation, have been implicated in antibody escape...
  37. ncbi Glycosylation and the immune system
    P M Rudd
    The Glycobiology Institute, Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UK
    Science 291:2370-6. 2001
    ..For example, in rheumatoid arthritis, an autoimmune disease, agalactosylated glycoforms of aggregated immunoglobulin G may induce association with the mannose-binding lectin and contribute to the pathology...
  38. pmc Mutations in the O-mannosyltransferase gene POMT1 give rise to the severe neuronal migration disorder Walker-Warburg syndrome
    Daniel Beltran-Valero de Bernabe
    Department of Human Genetics, University Medical Centre Nijmegen, Nijmegen, The Netherlands
    Am J Hum Genet 71:1033-43. 2002
    ..The protein products of POMGnT1 and Fukutin have both been implicated in protein glycosylation. To unravel the genetic basis of WWS, we first performed a genomewide linkage analysis in 10 consanguineous ..
  39. pmc The mannose-dependent epitope for neutralizing antibody 2G12 on human immunodeficiency virus type 1 glycoprotein gp120
    Rogier W Sanders
    Dept of Microbiology and Immunology, Weill Medical College of Cornell University, New York, NY 10021, USA
    J Virol 76:7293-305. 2002
    ....
  40. pmc Cross talk between O-GlcNAcylation and phosphorylation: roles in signaling, transcription, and chronic disease
    Gerald W Hart
    Departments of Biological Chemistry and Pediatrics, Johns Hopkins University, School of Medicine, Baltimore, Maryland 21205
    Annu Rev Biochem 80:825-58. 2011
    ..As tools for the study of O-GlcNAc become more facile and available, exponential growth in this area of research will eventually take place...
  41. doi Essential role of the glycosyltransferase sxc/Ogt in polycomb repression
    Maria Cristina Gambetta
    Gene Expression Programme, European Molecular Biology Laboratory EMBL, Meyerhofstrasse 1, 69117 Heidelberg, Germany
    Science 325:93-6. 2009
    ..Polycomb repression appears to be a critical function of Sxc/Ogt in Drosophila and may be mediated by the glycosylation of Polyhomeotic.
  42. pmc Deficiency of Dol-P-Man synthase subunit DPM3 bridges the congenital disorders of glycosylation with the dystroglycanopathies
    Dirk J Lefeber
    Laboratory of Pediatrics and Neurology, Institute for Genetic and Metabolic Disease, Radboud University Nijmegen Medical Centre, 6500 HB Nijmegen, The Netherlands
    Am J Hum Genet 85:76-86. 2009
    ..Isolated muscular dystrophy has not been described in the congenital disorders of glycosylation (CDG) caused by N-linked protein glycosylation defects...
  43. pmc Glycosylation focuses sequence variation in the influenza A virus H1 hemagglutinin globular domain
    Suman R Das
    NIAID, Bethesda, MA, USA
    PLoS Pathog 6:e1001211. 2010
    ..we address an important and largely overlooked issue in antigenic drift: how does the number and location of glycosylation sites affect HA evolution in man? We analyzed the glycosylation status of all full-length H1 subtype HA ..
  44. pmc Role of N-linked glycans in the functions of hepatitis C virus envelope glycoproteins
    Anne Goffard
    CNRS UPR2511, Institut de Biologie de Lille, France
    J Virol 79:8400-9. 2005
    Hepatitis C virus (HCV) encodes two viral envelope glycoproteins. E1 contains 4 or 5 N-linked glycosylation sites and E2 contains up to 11, with most of the sites being well conserved, suggesting that they play an essential role in some ..
  45. ncbi Virus glycosylation: role in virulence and immune interactions
    David J Vigerust
    Department of Pediatrics, Program in Vaccine Sciences, Vanderbilt University School of Medicine, Nashville, TN 37232, USA
    Trends Microbiol 15:211-8. 2007
    The study of N-linked glycosylation as it relates to virus biology has become an area of intense interest in recent years due to its ability to impart various advantages to virus survival and virulence...
  46. pmc Recapitulation of IVIG anti-inflammatory activity with a recombinant IgG Fc
    Robert M Anthony
    Laboratory of Molecular Genetics and Immunology, Rockefeller University, New York, NY 10021, USA
    Science 320:373-6. 2008
    ..This therapeutic molecule precisely defines the biologically active component of IVIG and helps guide development of an IVIG replacement with improved activity and availability...
  47. pmc Identification of a general O-linked protein glycosylation system in Acinetobacter baumannii and its role in virulence and biofilm formation
    Jeremy A Iwashkiw
    Alberta Glycomics Centre, Department of Biological Sciences, University of Alberta, Edmonton, Alberta, Canada
    PLoS Pathog 8:e1002758. 2012
    ..In this work we show that A. baumannii ATCC 17978 possesses an O-glycosylation system responsible for the glycosylation of multiple proteins...
  48. doi Molecular evolution and network-level analysis of the N-glycosylation metabolic pathway across primates
    Ludovica Montanucci
    Department of Experimental and Health Sciences, Institute of Evolutionary Biology, Universitat Pompeu Fabra Consejo Superior de Investigaciones Cientificas, Barcelona, Catalonia, Spain
    Mol Biol Evol 28:813-23. 2011
    N-glycosylation is one of the most important forms of protein modification, serving key biological functions in multicellular organisms...
  49. doi Specific sites of N-linked glycosylation on the hemagglutinin of H1N1 subtype influenza A virus determine sensitivity to inhibitors of the innate immune system and virulence in mice
    Michelle D Tate
    Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria 3010, Australia
    J Immunol 187:1884-94. 2011
    ..A reverse genetic approach was used to define the importance of particular N-glycosylation sites on H1 in determining sensitivity to innate immune defenses and virulence in mice...
  50. doi A novel role for the IgG Fc glycan: the anti-inflammatory activity of sialylated IgG Fcs
    Robert M Anthony
    The Laboratory of Molecular Genetics and Immunology, The Rockefeller University, New York, NY 10065, USA
    J Clin Immunol 30:S9-14. 2010
    ..This leads to upregulated surface expression of the inhibitory FcR, FcgammaRIIb, on inflammatory cells, thereby attenuating autoantibody-initiated inflammation...
  51. doi Mining the O-glycoproteome using zinc-finger nuclease-glycoengineered SimpleCell lines
    Catharina Steentoft
    Copenhagen Center for Glycomics, Department of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark
    Nat Methods 8:977-82. 2011
    ..the O-glycan elongation pathway in human cells, generating stable 'SimpleCell' lines with homogenous O-glycosylation. Three SimpleCell lines expressing only truncated GalNAcα or NeuAcα2-6GalNAcα O-glycans were produced, ..
  52. pmc A unique beta1,3-galactosyltransferase is indispensable for the biosynthesis of N-glycans containing Lewis a structures in Arabidopsis thaliana
    Richard Strasser
    Institute of Applied Genetics and Cell Biology, BOKU, University of Natural Resources and Applied Life Sciences, A 1190 Viena, Austria
    Plant Cell 19:2278-92. 2007
    ....
  53. pmc ISPD loss-of-function mutations disrupt dystroglycan O-mannosylation and cause Walker-Warburg syndrome
    Tobias Willer
    Department of Molecular Physiology and Biophysics, University of Iowa Roy J and Lucille A Carver College of Medicine, Iowa City, Iowa, USA
    Nat Genet 44:575-80. 2012
    ..Finally, we show that recessive mutations in ISPD abolish the initial step in laminin-binding glycan synthesis by disrupting dystroglycan O-mannosylation. This establishes a new mechanism for WWS pathophysiology...
  54. doi Location, location, location: new insights into O-GalNAc protein glycosylation
    David J Gill
    Institute of Molecular and Cell Biology IMCB, Proteos, 61 Biopolis Drive, Singapore, 138673
    Trends Cell Biol 21:149-58. 2011
    O-GalNAc glycosylation of proteins confers essential structural, protective and signaling roles in eumetazoans...
  55. pmc Longer V1V2 region with increased number of potential N-linked glycosylation sites in the HIV-1 envelope glycoprotein protects against HIV-specific neutralizing antibodies
    Marit J van Gils
    Department of Experimental Immunology, Academic Medical Center, M01 120, Meibergdreef 9, 1105 AZ Amsterdam, Netherlands
    J Virol 85:6986-95. 2011
    ..and at a population level, coincided with longer variable loops and an increased number of potential N-linked glycosylation sites (PNGS) in the viral envelope glycoprotein (Env) and, in particular, in variable regions 1 and 2 (V1V2)...
  56. pmc Mutations in ISPD cause Walker-Warburg syndrome and defective glycosylation of α-dystroglycan
    Tony Roscioli
    Department of Human Genetics, Nijmegen Centre for Molecular Life Sciences, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    Nat Genet 44:581-5. 2012
    ..by complex eye and brain abnormalities with congenital muscular dystrophy (CMD) and aberrant a-dystroglycan glycosylation. Here we report mutations in the ISPD gene (encoding isoprenoid synthase domain containing) as the second most ..
  57. doi Folding of glycoproteins: toward understanding the biophysics of the glycosylation code
    Dalit Shental-Bechor
    Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel
    Curr Opin Struct Biol 19:524-33. 2009
    b>Glycosylation is among the most common post-translational modifications that proteins undergo that may affect many of their activities...
  58. pmc Engineering mammalian mucin-type O-glycosylation in plants
    Zhang Yang
    Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Aarhus University, Flakkebjerg, 4200 Slagelse, Denmark
    J Biol Chem 287:11911-23. 2012
    Mucin-type O-glycosylation is an important post-translational modification that confers a variety of biological properties and functions to proteins...
  59. pmc Envelope deglycosylation enhances antigenicity of HIV-1 gp41 epitopes for both broad neutralizing antibodies and their unmutated ancestor antibodies
    Ben Jiang Ma
    Duke Human Vaccine Institute, Duke University School of Medicine, Durham, North Carolina, United States of America
    PLoS Pathog 7:e1002200. 2011
    ....
  60. doi Neisseria gonorrhoeae pilin glycan contributes to CR3 activation during challenge of primary cervical epithelial cells
    Michael P Jennings
    Institute for Glycomics, Griffith University, Gold Coast Campus, Australia
    Cell Microbiol 13:885-96. 2011
    ..an O-linked glycan, which may be present as a di- or monosaccharide because of phase variation of select pilin glycosylation genes...
  61. pmc Comparison of methods for profiling O-glycosylation: Human Proteome Organisation Human Disease Glycomics/Proteome Initiative multi-institutional study of IgA1
    Yoshinao Wada
    Osaka Medical Center and Research Institute for Maternal and Child Health, Izumi, Osaka, Japan
    Mol Cell Proteomics 9:719-27. 2010
    ..In addition, mass spectrometric methodologies to analyze O-glycopeptides were also successful...
  62. pmc Glycosylation status of the C. albicans cell wall affects the efficiency of neutrophil phagocytosis and killing but not cytokine signaling
    Chirag C Sheth
    The Aberdeen Fungal Group, School of Medical Sciences, Institute of Medical Sciences, University of Aberdeen, Aberdeen, UK
    Med Mycol 49:513-24. 2011
    ..albicans by human PMNs was therefore investigated. N- and O-glycosylation-deficient mutants were attenuated in binding and phagocytosis by PMNs and this was associated with reduced ..
  63. doi Analysis of immunoglobulin glycosylation by LC-ESI-MS of glycopeptides and oligosaccharides
    Johannes Stadlmann
    Department of Chemistry, University of Natural Resources and Applied Life Sciences BOKU, Vienna, Austria
    Proteomics 8:2858-71. 2008
    Two LC-ESI-MS methods for the analysis of antibody glycosylation are presented. In the first approach, tryptic glycopeptides are separated by RP chromatography and analyzed by ESI-MS...
  64. doi Optimal and consistent protein glycosylation in mammalian cell culture
    Patrick Hossler
    Process Sciences Upstream, Technical Operations, Bristol Myers Squibb Company, East Syracuse, NY 13057, USA
    Glycobiology 19:936-49. 2009
    ..b>Glycosylation is a critical protein quality attribute that can modulate the efficacy of a commercial therapeutic ..
  65. doi A novel cerebello-ocular syndrome with abnormal glycosylation due to abnormalities in dolichol metabolism
    Eva Morava
    Radboud University Nijmegen Medical Centre, Institute for Genetic and Metabolic Disease, P O Box 9101, 6500 HB Nijmegen, The Netherlands
    Brain 133:3210-20. 2010
    ..and slowly progressive ophthalmological symptoms are common features in patients with congenital disorders of glycosylation type I...
  66. pmc Malectin: a novel carbohydrate-binding protein of the endoplasmic reticulum and a candidate player in the early steps of protein N-glycosylation
    Thomas Schallus
    European Molecular Biology Laboratory, 69117 Heidelberg, Germany
    Mol Biol Cell 19:3404-14. 2008
    N-Glycosylation starts in the endoplasmic reticulum (ER) where a 14-sugar glycan composed of three glucoses, nine mannoses, and two N-acetylglucosamines (Glc(3)Man(9)GlcNAc(2)) is transferred to nascent proteins...
  67. ncbi Optimization of humanized IgGs in glycoengineered Pichia pastoris
    Huijuan Li
    GlycoFi Inc 21 Lafayette Street, Lebanon, New Hampshire 03766, USA
    Nat Biotechnol 24:210-5. 2006
    ..native state and all clinically approved mAbs are produced by mammalian cell lines, which secrete mAbs with glycosylation structures that are similar, but not identical, to their human counterparts...
  68. pmc Mammalian glycosylation in immunity
    Jamey D Marth
    Department of Cellular and Molecular Medicine, The Howard Hughes Medical Institute, University of California San Diego, La Jolla, California 92093, USA
    Nat Rev Immunol 8:874-87. 2008
    b>Glycosylation produces a diverse and abundant repertoire of glycans, which are collectively known as the glycome...
  69. pmc Class I alpha-mannosidases are required for N-glycan processing and root development in Arabidopsis thaliana
    Eva Liebminger
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, A 1190 Viena, Austria
    Plant Cell 21:3850-67. 2009
    ..These findings show that class I alpha-mannosidases are essential for early N-glycan processing and play a role in root development and cell wall biosynthesis in Arabidopsis...
  70. pmc Variable loop glycan dependency of the broad and potent HIV-1-neutralizing antibodies PG9 and PG16
    Katie J Doores
    Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA 92037, USA
    J Virol 84:10510-21. 2010
    ..Antibody neutralization has been shown to be dependent on the presence of N-linked glycosylation at position 160 in gp120...
  71. pmc In planta protein sialylation through overexpression of the respective mammalian pathway
    Alexandra Castilho
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, Muthgasse 18, 1190 Vienna, Austria
    J Biol Chem 285:15923-30. 2010
    ..Sialylation was at great uniformity when glycosylation mutants that lack plant-specific N-glycan residues were used as expression hosts...
  72. pmc Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion
    M Flamand
    Unité des Arbovirus et Virus des Fièvres Hémorragiques, Institut Pasteur, 75724 Paris Cedex 15, 91198 Gif sur Yvette Cedex, France
    J Virol 73:6104-10. 1999
    ..These results, together with observations related to the absence of secretion of NS1 from Den-infected insect cells, suggest that maturation and secretion of hexameric NS1 depend on the glycosylation status of the host cell.
  73. doi A systematic approach to protein glycosylation analysis: a path through the maze
    Karina Mariño
    Dublin Oxford Glycobiology Laboratory, National Institute for Bioprocessing, Research and Training, University College Dublin Conway Institute, University College Dublin, Belfield, Ireland
    Nat Chem Biol 6:713-23. 2010
    Protein glycosylation is an important post-translational modification. It is a feature that enhances the functional diversity of proteins and influences their biological activity...
  74. ncbi Two novel types of O-glycans on the mugwort pollen allergen Art v 1 and their role in antibody binding
    Renaud Leonard
    Division of Biochemistry, Department of Chemistry, Universitaet fuer Bodenkultur Wien, Muthgasse 18, A 1190 Vienna, Austria
    J Biol Chem 280:7932-40. 2005
    ..sera of some allergic patients react with natural but not with recombinant Art v 1 produced in bacteria, the glycosylation of Art v 1 may play a role in IgE binding and human allergic reactions...
  75. ncbi Dynamic interplay between O-glycosylation and O-phosphorylation of nucleocytoplasmic proteins: alternative glycosylation/phosphorylation of THR-58, a known mutational hot spot of c-Myc in lymphomas, is regulated by mitogens
    Kazuo Kamemura
    Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 2185, USA
    J Biol Chem 277:19229-35. 2002
    ..of Ser-62 to Ala showed a marked decrease of Thr-58 phosphorylation and a marked increase of Thr-58 glycosylation. Growth inhibition of HL60 cells by serum starvation increases Thr-58 glycosylation and correspondingly ..
  76. pmc N-linked glycosylation attenuates H3N2 influenza viruses
    David J Vigerust
    St Jude Children s Research Hospital, 332 N Lauderdale Street, Memphis, TN 38105 2794, USA
    J Virol 81:8593-600. 2007
    ..the last four decades, H3N2 subtype influenza A viruses have gradually acquired additional potential sites for glycosylation within the globular head of the hemagglutinin (HA) protein...
  77. pmc Reciprocal phosphorylation and glycosylation recognition motifs control NCAPP1 interaction with pumpkin phloem proteins and their cell-to-cell movement
    Ken Ichiro Taoka
    Section of Plant Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA
    Plant Cell 19:1866-84. 2007
    ..Protein overlay assays and coimmunoprecipitation experiments established that phosphorylation and glycosylation, on both Nicotiana tabacum NON-CELL-AUTONOMOUS PATHWAY PROTEIN1 (Nt-NCAPP1) and the phloem NCAPs, are ..
  78. pmc Selection for human immunodeficiency virus type 1 envelope glycosylation variants with shorter V1-V2 loop sequences occurs during transmission of certain genetic subtypes and may impact viral RNA levels
    Bhavna Chohan
    Division of Human Biology, Fred Hutchinson Cancer Center, 1100 Fairview Ave N, C3 168 Seattle, WA 98109 1024, USA
    J Virol 79:6528-31. 2005
    ..envelopes have signature sequences that include shorter V1-V2 loop sequences and fewer predicted N-linked glycosylation sites relative to the overall population of circulating variants...
  79. pmc Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous beta1,4-galactosylated N-glycan profile
    Richard Strasser
    Department of Applied Genetics and Cell Biology, University of Natural Resources and Applied Life Sciences, 1190 Vienna, Austria
    J Biol Chem 284:20479-85. 2009
    It is well established that proper N-glycosylation significantly influences the efficacy of monoclonal antibodies (mAbs)...
  80. ncbi Negative regulation of T-cell activation and autoimmunity by Mgat5 N-glycosylation
    M Demetriou
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada
    Nature 409:733-9. 2001
    ..Here we demonstrate that a deficiency in beta1,6 N-acetylglucosaminyltransferase V (Mgat5), an enzyme in the N-glycosylation pathway, lowers T-cell activation thresholds by directly enhancing TCR clustering...
  81. pmc POMT2 mutations cause alpha-dystroglycan hypoglycosylation and Walker-Warburg syndrome
    J van Reeuwijk
    Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
    J Med Genet 42:907-12. 2005
    ..WWS patients show defective O-glycosylation of alpha-dystroglycan (alpha-DG), which plays a key role in bridging the cytoskeleton of muscle and CNS cells ..
  82. ncbi Structure of an HIV gp120 envelope glycoprotein in complex with the CD4 receptor and a neutralizing human antibody
    P D Kwong
    Department of Biochemistry and Molecular Biophysics, Columbia University, New York, New York 10032, USA
    Nature 393:648-59. 1998
    ..Our results provide a framework for understanding the complex biology of HIV entry into cells and should guide efforts to intervene...
  83. pmc O-GlcNAcylation regulates phosphorylation of tau: a mechanism involved in Alzheimer's disease
    Fei Liu
    Department of Neurochemistry, New York State Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, NY 10314, USA
    Proc Natl Acad Sci U S A 101:10804-9. 2004
    ..Here, we demonstrate that human brain tau was modified by O-GlcNAcylation, a type of protein O-glycosylation by which the monosaccharide beta-N-acetylglucosamine (GlcNAc) attaches to serine/threonine residues via an O-..
  84. pmc Use of combinatorial genetic libraries to humanize N-linked glycosylation in the yeast Pichia pastoris
    Byung Kwon Choi
    Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA
    Proc Natl Acad Sci U S A 100:5022-7. 2003
    The secretory pathway of Pichia pastoris was genetically re-engineered to perform sequential glycosylation reactions that mimic early processing of N-glycans in humans and other higher mammals...
  85. ncbi Glycosylation in cellular mechanisms of health and disease
    Kazuaki Ohtsubo
    Howard Hughes Medical Institute and Department of Cellular and Molecular Medicine, 9500 Gilman Drive MC0625, University of California, San Diego, La Jolla, CA 92093, USA
    Cell 126:855-67. 2006
    b>Glycosylation produces an abundant, diverse, and highly regulated repertoire of cellular glycans that are frequently attached to proteins and lipids...
  86. ncbi The role of protein glycosylation in allergy
    Friedrich Altmann
    Divison of Biochemistry, Department of Chemistry, University of Natural Resources and Applied Life Sciences, Vienna, Austria
    Int Arch Allergy Immunol 142:99-115. 2007
    ..However, currently the occurrence of this O-glycan determinant appears to be rather restricted...
  87. ncbi Refining genotype phenotype correlations in muscular dystrophies with defective glycosylation of dystroglycan
    Caroline Godfrey
    Dubowitz Neuromuscular Unit, Hammersmith Hospital, Imperial College, London, UK
    Brain 130:2725-35. 2007
    Muscular dystrophies with reduced glycosylation of alpha-dystroglycan (alpha-DG), commonly referred to as dystroglycanopathies, are a heterogeneous group of autosomal recessive conditions which include a wide spectrum of clinical ..
  88. pmc A comparative study of alpha-dystroglycan glycosylation in dystroglycanopathies suggests that the hypoglycosylation of alpha-dystroglycan does not consistently correlate with clinical severity
    Cecilia Jimenez-Mallebrera
    Dubowitz Neuromuscular Centre, Institute of Child Health and Great Ormond Street Hospital for Children, UCL, London, UK
    Brain Pathol 19:596-611. 2009
    ..These data indicate that it is not always possible to correlate clinical course and alpha-dystroglycan labeling and suggest that there might be differences in alpha-dystroglycan processing in these disorders...
  89. doi The role of N-acetylglucosaminyltransferase III and V in the post-transcriptional modifications of E-cadherin
    Salomé S Pinho
    Institute of Molecular Pathology and Immunology of the University of Porto IPATIMUP, Rua Dr Roberto Frias s n, 4200 465 Porto, Portugal
    Hum Mol Genet 18:2599-608. 2009
    ..This study opens new insights into the post-transcriptional modifications of E-cadherin in its biological function, in a tumor context...
  90. pmc Membrane topology of NAADP-sensitive two-pore channels and their regulation by N-linked glycosylation
    Robert Hooper
    Department of Cell and Developmental Biology, University College London, London WC1E 6BT, United Kingdom
    J Biol Chem 286:9141-9. 2011
    ..Both TPC1 and TPC2 are N-glycosylated with residues 599, 611, and 616 contributing to glycosylation of TPC1...
  91. doi Fc-glycosylation influences Fcγ receptor binding and cell-mediated anti-HIV activity of monoclonal antibody 2G12
    Donald N Forthal
    Division of Infectious Diseases, Department of Medicine, University of California, Irvine School of Medicine, Irvine, CA 92697, USA
    J Immunol 185:6876-82. 2010
    ..the nature of the glycan structure on the Fc domain is a critical determinant of Fc-FcγR binding, proper Fc glycosylation may contribute to Ab-mediated protection...
  92. pmc IgA1-secreting cell lines from patients with IgA nephropathy produce aberrantly glycosylated IgA1
    Hitoshi Suzuki
    University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    J Clin Invest 118:629-39. 2008
    Aberrant glycosylation of IgA1 plays an essential role in the pathogenesis of IgA nephropathy. This abnormality is manifested by a deficiency of galactose in the hinge-region O-linked glycans of IgA1...
  93. pmc Aberrant IgA1 glycosylation is inherited in familial and sporadic IgA nephropathy
    Ali G Gharavi
    Department of Medicine, Columbia University College of Physicians and Surgeons, 630 W 168th Street, P and S 10 445 New York, New York 10032, USA
    J Am Soc Nephrol 19:1008-14. 2008
    ..It is not known whether this aberrant glycosylation is the result of an acquired or inherited defect, or whether the presence of aberrant IgA1 glycoforms alone ..
  94. doi Congenital disorders of glycosylation: an update on defects affecting the biosynthesis of dolichol-linked oligosaccharides
    Micha A Haeuptle
    Institute of Physiology, University of Zurich, Zurich, Switzerland
    Hum Mutat 30:1628-41. 2009
    Defects in the biosynthesis of the oligosaccharide precursor for N-glycosylation lead to decreased occupancy of glycosylation sites and thereby to diseases known as congenital disorders of glycosylation (CDG)...
  95. doi Critical roles of mucin 1 glycosylation by transactivated polypeptide N-acetylgalactosaminyltransferase 6 in mammary carcinogenesis
    Jae Hyun Park
    Laboratory of Molecular Medicine, Human Genome Center, Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    Cancer Res 70:2759-69. 2010
    ..a great majority of breast cancers and encodes a glycosyltransferase responsible for initiating mucin-type O-glycosylation. Knockdown of GALNT6 by small interfering RNA significantly enhanced cell adhesion function and suppressed the ..
  96. doi Lectin-based proteomic profiling of aged skeletal muscle: decreased pyruvate kinase isozyme M1 exhibits drastically increased levels of N-glycosylation
    Kathleen O'Connell
    Department of Biology, National University of Ireland, Maynooth, Co Kildare, Ireland
    Eur J Cell Biol 87:793-805. 2008
    Since various neuromuscular diseases are associated with abnormal glycosylation, it was of interest to determine whether this key post-translational modification is also altered in aged skeletal muscle...
  97. pmc Functional characterization of flagellin glycosylation in Campylobacter jejuni 81-176
    Cheryl P Ewing
    Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Ave, Silver Spring, MD 20910, USA
    J Bacteriol 191:7086-93. 2009
    ..jejuni strain 81-176, FlaA, has been shown to be glycosylated at 19 serine or threonine sites, and this glycosylation is required for flagellar filament formation. Some enzymatic components of the glycosylation machinery of C...
  98. doi Adaptive regulation at the cell surface by N-glycosylation
    James W Dennis
    Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue R988, Toronto, ON, Canada M5G 1X5
    Traffic 10:1569-78. 2009
    ..Computational modelling of the hexosamine/Golgi/lattice has provided new insight on cell surface adaptation in cancer and autoimmune disease...
  99. ncbi Mutants in DEFECTIVE GLYCOSYLATION, an Arabidopsis homolog of an oligosaccharyltransferase complex subunit, show protein underglycosylation and defects in cell differentiation and growth
    Olivier Lerouxel
    Unité Mixte de Recherche 6037 Centre National de la Recherche Scientifique, IFRMP 23, Faculte des Sciences, Universite de Rouen, 76821 Mont Saint Aignan Cedex, France
    Plant J 42:455-68. 2005
    ..with the known function of the OST complex in eukaryotes, the dgl1-1 mutation led to a reduced N-linked glycosylation of the ER-resident protein disulfide isomerase. A second more severe mutant (dgl1-2) was embryo-lethal...
  100. ncbi Patients with IgA nephropathy have increased serum galactose-deficient IgA1 levels
    Z Moldoveanu
    Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama 35294, USA
    Kidney Int 71:1148-54. 2007
    ..5%, with specificity 94%; the positive predictive value was 88.6% and the negative predictive value was 78.9%. We conclude that this lectin-binding assay may have potential as a noninvasive diagnostic test for IgA nephropathy...
  101. ncbi Screening for N-glycosylated proteins by liquid chromatography mass spectrometry
    Jakob Bunkenborg
    MDS Proteomics Odense, Denmark
    Proteomics 4:454-65. 2004
    ..only one change can be associated with a given residue, many different oligosaccharides can be attached to a glycosylation site residue...

Research Grants96

  1. STRUCTURE, BIOSYNTHESIS AND FUNCTION OF GLYCOPROTEINS
    Stuart Kornfeld; Fiscal Year: 2007
    ..that determine binding to phosphotransferase and analyze how these residues direct phosphorylation at selected glycosylation sites...
  2. ASSEMBLY AND TRANSFER OF N-LINKED OLIGOSACCHARIDE
    James Gilmore; Fiscal Year: 1993
    The research described here is focused upon asparagine-linked glycosylation of newly synthesized proteins in the rough endoplasmic reticulum...
  3. Development of A Novel Strategy to Produce Antibacterial Glycoconjugate Vaccines
    Peng George Wang; Fiscal Year: 2010
    ..The objective of this application is to explore two recently established bacterial protein glycosylation systems to obtain glycoconjugate vaccines in a facile, efficient, and easily applicable manner...
  4. Glycoregulation of Skp1 in the cytoplasm and nucleus
    Christopher West; Fiscal Year: 2009
    ..a specific example of a type that has seemingly been borrowed from the secretory pathway of eukaryotic cells, glycosylation, but might actually have first evolved in the cytoplasm of bacterial cells...
  5. Glycoregulation of Skp1 in the cytoplasm and nucleus
    Christopher M West; Fiscal Year: 2010
    ..a specific example of a type that has seemingly been borrowed from the secretory pathway of eukaryotic cells, glycosylation, but might actually have first evolved in the cytoplasm of bacterial cells...
  6. A novel transgenic silkworm system for recombinant glycoprotein production
    Donald L Jarvis; Fiscal Year: 2010
    ..The major impediment is that the endogenous protein glycosylation pathways of the silk gland cannot be expected to properly glycosylate higher eukaryotic glycoproteins...
  7. A novel transgenic silkworm system for recombinant glycoprotein production
    Donald Jarvis; Fiscal Year: 2009
    ..The major impediment is that the endogenous protein glycosylation pathways of the silk gland cannot be expected to properly glycosylate higher eukaryotic glycoproteins...
  8. A novel transgenic silkworm system for recombinant glycoprotein production
    Donald Jarvis; Fiscal Year: 2007
    ..The major impediment is that the endogenous protein glycosylation pathways of the silk gland cannot be expected to properly glycosylate higher eukaryotic glycoproteins...
  9. ROBBINS Glycosylation and Glycosidases-Cell and Molecular Biology
    Phillips W Robbins; Fiscal Year: 2010
    ..Second, there is positive selection for N-linked glycosylation in eukaryotes with N- glycan-dependent QC...
  10. A SARS-CoV Spike Protein Vaccine
    B Sim; Fiscal Year: 2005
    ..Region I was selected in part because of paucity of N-glycosylation sites. However, there are 9 such sites in the native sequence...
  11. HA Surface Presented Yeast Flu Vaccine and Its Enhancement by CD154 Codisplay
    Kaiming Ye; Fiscal Year: 2009
    ..Although yeast has different glycosylation capability as compared to mammalian cells, this limited glycosylation may actually be a benefit to formulating ..
  12. HA Surface Presented Yeast Flu Vaccine and Its Enhancement by CD154 Codisplay
    Kaiming Ye; Fiscal Year: 2010
    ..Although yeast has different glycosylation capability as compared to mammalian cells, this limited glycosylation may actually be a benefit to formulating ..
  13. N-Glycosylation And ER Stress
    MARK LEHRMAN; Fiscal Year: 2009
    ..First, there are 13 human genetic diseases in the family "Congenital Disorders of Glycosylation" (CDG) Type I, with defective LLO synthesis...
  14. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2010
    ..We find that metabolite availability to the Golgi N- glycosylation pathway exerts autonomous control over the assembly of macromolecular complexes on the cell surface, and in ..
  15. Regulation of structure and function of protein by glycosylation
    Zhiwen Zhang; Fiscal Year: 2009
    Our long-term goal is to understand the various regulatory roles of glycosylation. We are specifically interested in O-N-acetylglucosamine (O-GlcNAc) modification, where the GlcNAc moiety is attached by an ether linkage to a serine or ..
  16. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2010
    ..We find that metabolite availability to the Golgi N- glycosylation pathway exerts autonomous control over the assembly of macromolecular complexes on the cell surface, and in ..
  17. Regulation of structure and function of protein by glycosylation
    Zhiwen Jonathan Zhang; Fiscal Year: 2010
    Our long-term goal is to understand the various regulatory roles of glycosylation. We are specifically interested in O-N-acetylglucosamine (O-GlcNAc) modification, where the GlcNAc moiety is attached by an ether linkage to a serine or ..
  18. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2009
    ..Numerous studies have suggested that IgAN results from abnormal O-glycosylation of IgA1...
  19. Genetic Basis of Non-Familial IgA Nephropathy
    Tongzhong Ju; Fiscal Year: 2010
    ..Numerous studies have suggested that IgAN results from abnormal O-glycosylation of IgA1...
  20. Glycosylation & Function of an Oral Streptococcal Adhesin
    Hui Wu; Fiscal Year: 2007
    ..b>Glycosylation of Fapl is important for development of mature biofilm...
  21. Glycosylation & Function of an Oral Streptococcal Adhesin
    Hui Wu; Fiscal Year: 2010
    ..b>Glycosylation of Fapl is important for development of mature biofilm...
  22. Glycosylation & Function of an Oral Streptococcal Adhesin
    Hui Wu; Fiscal Year: 2009
    ..b>Glycosylation of Fapl is important for development of mature biofilm...
  23. Glycosylation & Function of an Oral Streptococcal Adhesin
    Hui Wu; Fiscal Year: 2009
    ..b>Glycosylation of Fapl is important for development of mature biofilm...
  24. Amidoglycosylation Reactions of Glycal Metallanitrenes
    Christian Rojas; Fiscal Year: 2007
    ..approach uses glycal 3-carbamates as starting materials and proceeds through a tandem alkene amidation-glycosylation sequence-amidoglycosylation-to introduce nitrogen at C2 of the sugar framework and to establish an anomeric ..
  25. CYTOPLASMIC FUCOSYLATION
    Christopher West; Fiscal Year: 1993
    Mutations in the glycosylation of proteins have dramatic effects on cell proliferation rate, cell sorting, pseudoplasmodial migration, spore coat formation, spore germination, and protein transport rate, in the cellular slime mold ..
  26. NUCLEOTIDE DONORS AFFECTING GLYCOSYLATION PATHWAYS
    Ajit Varki; Fiscal Year: 2003
    All living cells synthesize glycan chains. The process of glycosylation is catalyzed by highly specific glycosyltransferases that require specific sugar nucleotide donors...
  27. Glycoregulation of Skp1 in the cytoplasm and nucleus
    Christopher M West; Fiscal Year: 2010
    ..a specific example of a type that has seemingly been borrowed from the secretory pathway of eukaryotic cells, glycosylation, but might actually have first evolved in the cytoplasm of bacterial cells...
  28. TREATMENT OF BCR/ABL CAUSED LEUKEMIAS WITH FTIs
    Nora Heisterkamp; Fiscal Year: 2009
    ..Protein glycosylation takes place on a massive scale but has been largely neglected because of the difficulty in studying and ..
  29. Model Vaccines Exploiting Fungal Mannosylation
    STUART LEVITZ; Fiscal Year: 2009
    ..Cryptococcal MP contain serine/threonine (S/T)-rich regions which serve as sites for extensive O-linked glycosylation as well as N-X-S/T sequons that serve as N-glycosylation sites...
  30. Regulation of IGF-1 and Insulin Signaling by O-GlcNAc Glycosylation
    Lauren E Ball; Fiscal Year: 2010
    ..IRS-1 and IRS-2 are also glycosylated at Ser/Thr residues by N-acetylglucosamine (O-GlcNAc). This unique glycosylation is a reversible posttranslational modification proposed to regulate protein function in a manner analogous to ..
  31. GLYCOSYLATION DEFECTS OF LECTIN-RESISTANT TUMOR CELLS
    Pamela Stanley; Fiscal Year: 1993
    ..To address these questions we have isolated glycosylation-defective Chinese hamster ovary (CHO) cell mutants and used them to define the pathways of carbohydrate ..
  32. O-GLYCOSYLATION OF EPIDERMAL GROWTH FACTOR-LIKE MODULES
    Robert S Haltiwanger; Fiscal Year: 2011
    ..up to 36 tandem epidermal growth factor-like (EGF) repeats, many of which are modified by two unusual forms of glycosylation: O-fucose and O-glucose. O-Fucose modifications are essential for Notch function...
  33. O-GLYCOSYLATION OF EPIDERMAL GROWTH FACTOR-LIKE MODULES
    Robert S Haltiwanger; Fiscal Year: 2010
    ..up to 36 tandem epidermal growth factor-like (EGF) repeats, many of which are modified by two unusual forms of glycosylation: O-fucose and O-glucose. O-Fucose modifications are essential for Notch function...
  34. Molecular biology of TPPI/CLN2 protein
    Adam Golabek; Fiscal Year: 2006
    ..We propose to further elucidate the role of N-glycosylation in the trafficking of TPP I as well as to determine whether and how N-glycosylation affects folding, activity, ..