sperm ovum interactions

Summary

Summary: Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.

Top Publications

  1. ncbi Platelet activating factor improves the in vitro penetration of zona free hamster eggs by buffalo (Bubalus bubalis) spermatozoa
    Subodh Kumar
    Biotechnology Laboratory, National Dairy Research Institute, Karnal 132 001, India
    Theriogenology 63:1564-72. 2005
  2. ncbi The gamete fusion process is defective in eggs of Cd9-deficient mice
    K Kaji
    Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan
    Nat Genet 24:279-82. 2000
  3. ncbi SAMP14, a novel, acrosomal membrane-associated, glycosylphosphatidylinositol-anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm-egg interaction
    Jagathpala Shetty
    Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 278:30506-15. 2003
  4. ncbi Remodelling the paternal chromatin at fertilization in mammals
    David W McLay
    Departments of Obstetrics and Gynecology, Biology and Medicine, McGill University, Room F350, Royal Victoria Hospital, 687 Pine Ave W, Montreal H3A 1A1, Canada
    Reproduction 125:625-33. 2003
  5. ncbi Spatiotemporal analysis of Ca(2+) waves in relation to the sperm entry site and animal-vegetal axis during Ca(2+) oscillations in fertilized mouse eggs
    R Deguchi
    Laboratory of Intracellular Metabolism, Department of Molecular Physiology, National Institute for Physiological Sciences, Myodaiji cho, Okazaki, 444 8585, Japan
    Dev Biol 218:299-313. 2000
  6. ncbi Are Src family kinases involved in cell cycle resumption in rat eggs?
    A Talmor-Cohen
    Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
    Reproduction 127:455-63. 2004
  7. ncbi A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57
    Diego A Ellerman
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Davis, California 95616, USA
    Dev Cell 10:831-7. 2006
  8. ncbi Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes
    L J Cox
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Reproduction 124:611-23. 2002
  9. ncbi Evidence for distinct serine protease activities with a potential role in processing the sperm protein fertilin
    L Lum
    Program in Cellular Biochemistry and Biophysics, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Dev Biol 191:131-45. 1997
  10. ncbi Cyclic FEE peptide increases human gamete fusion and potentiates its RGD-induced inhibition
    A Ziyyat
    Laboratoire de Biologie de la Reproduction, UPRES 3410, UFR SMBH, Universite Paris 13, 74, rue Marcel Cachin, 93017 Bobigny, Service d Histologie Embryologie Cytogénétique, Hôpital Jean Verdier Assistance Publique Hôpitaux de Paris, Bondy, France
    Hum Reprod 20:3452-8. 2005

Detail Information

Publications198 found, 100 shown here

  1. ncbi Platelet activating factor improves the in vitro penetration of zona free hamster eggs by buffalo (Bubalus bubalis) spermatozoa
    Subodh Kumar
    Biotechnology Laboratory, National Dairy Research Institute, Karnal 132 001, India
    Theriogenology 63:1564-72. 2005
    ..18, (14.90); 0.79 +/- 0.02, 1.10 +/- 0.03, (0.31) and 5.22 +/- 1.22, 21.69 +/- 1.88, (16.47)%, respectively. In conclusion, PAF significantly increased the AR and other aspects of fertilization, despite a small reduction in motility...
  2. ncbi The gamete fusion process is defective in eggs of Cd9-deficient mice
    K Kaji
    Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan
    Nat Genet 24:279-82. 2000
    ..In normal animals, Cd9 molecules were expressed on the egg microvilli and became densely concentrated at the sperm attachment site. Thus, our results show that Cd9 is important in the gamete fusion process at fertilization...
  3. ncbi SAMP14, a novel, acrosomal membrane-associated, glycosylphosphatidylinositol-anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm-egg interaction
    Jagathpala Shetty
    Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, Virginia 22908, USA
    J Biol Chem 278:30506-15. 2003
    ..SAMP14 represents a GPI-anchored putative receptor in the Ly-6/uPAR family that is exposed on the inner acrosomal membrane after the acrosome reaction...
  4. ncbi Remodelling the paternal chromatin at fertilization in mammals
    David W McLay
    Departments of Obstetrics and Gynecology, Biology and Medicine, McGill University, Room F350, Royal Victoria Hospital, 687 Pine Ave W, Montreal H3A 1A1, Canada
    Reproduction 125:625-33. 2003
    ..Identifying the mechanisms that underlie sperm chromatin remodelling at fertilization may be relevant for understanding reprogramming of somatic cell nuclei after transfer into oocytes...
  5. ncbi Spatiotemporal analysis of Ca(2+) waves in relation to the sperm entry site and animal-vegetal axis during Ca(2+) oscillations in fertilized mouse eggs
    R Deguchi
    Laboratory of Intracellular Metabolism, Department of Molecular Physiology, National Institute for Physiological Sciences, Myodaiji cho, Okazaki, 444 8585, Japan
    Dev Biol 218:299-313. 2000
    ..Since the shift of the wave initiation site to the vegetal cortex is observed in fertilized eggs of nemertean worms and ascidians, this might be an evolutionarily conserved feature...
  6. ncbi Are Src family kinases involved in cell cycle resumption in rat eggs?
    A Talmor-Cohen
    Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
    Reproduction 127:455-63. 2004
    ..The possible involvement of SFKs in the signal transduction pathways that lead from the sperm-egg fusion site downstream of the Ca2+ release remains unclear...
  7. ncbi A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57
    Diego A Ellerman
    Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Davis, California 95616, USA
    Dev Cell 10:831-7. 2006
    ..The results support the idea that thiol-disulfide exchange is a mechanism that may act in gamete fusion to produce conformational changes in fusion-active proteins...
  8. ncbi Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytes
    L J Cox
    Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
    Reproduction 124:611-23. 2002
    ..These results are consistent with the proposal that sperm PLCzeta is the molecular trigger for egg activation during fertilization and that the role and activity of PLCzeta is highly conserved across mammalian species...
  9. ncbi Evidence for distinct serine protease activities with a potential role in processing the sperm protein fertilin
    L Lum
    Program in Cellular Biochemistry and Biophysics, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
    Dev Biol 191:131-45. 1997
    ....
  10. ncbi Cyclic FEE peptide increases human gamete fusion and potentiates its RGD-induced inhibition
    A Ziyyat
    Laboratoire de Biologie de la Reproduction, UPRES 3410, UFR SMBH, Universite Paris 13, 74, rue Marcel Cachin, 93017 Bobigny, Service d Histologie Embryologie Cytogénétique, Hôpital Jean Verdier Assistance Publique Hôpitaux de Paris, Bondy, France
    Hum Reprod 20:3452-8. 2005
    ..We investigated, in humans, whether oocyte integrins could act similarly in gamete fusion, using a cyclic peptide containing the putative disintegrin-binding domain of human fertilin beta [cyclic FEE (cFEE)] and RGD peptide...
  11. ncbi SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated protein
    Zhonglin Hao
    Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, Virginia 22908, USA
    Biol Reprod 66:735-44. 2002
    ..Recombinant SAMP32 reacted with serum from an infertile man, suggesting that it is isoantigenic. Antibodies against recombinant SAMP32 inhibited both the binding and the fusion of human sperm to zona-free hamster eggs...
  12. ncbi Egg activation at fertilization: where it all begins
    Linda L Runft
    Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
    Dev Biol 245:237-54. 2002
    ....
  13. ncbi Contribution of mouse egg zona pellucida glycoproteins to gamete recognition during fertilization
    Paul M Wassarman
    Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, New York, USA
    J Cell Physiol 204:388-91. 2005
    ....
  14. ncbi Mediation of sperm-egg fusion: evidence that mouse egg alpha6beta1 integrin is the receptor for sperm fertilinbeta
    H Chen
    Department of Chemistry, State University of New York, Stony Brook 11794 3400, USA
    Chem Biol 6:1-10. 1999
    ..We set out to identify the fertilinbeta binding partner on the egg plasma membrane...
  15. ncbi Identification of mouse sperm SED1, a bimotif EGF repeat and discoidin-domain protein involved in sperm-egg binding
    Michael A Ensslin
    Department of Cell Biology, Whitehead Biomedical Research Building, Emory University School of Medicine, 615 Michael Street, Room 405, Atlanta, GA 30322, USA
    Cell 114:405-17. 2003
    ..These studies illustrate that Notch-like EGF and discoidin/C domains, protein motifs that facilitate a variety of cellular interactions, participate in gamete recognition as well...
  16. doi The dynamics of calcium oscillations that activate mammalian eggs
    Karl Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
    Int J Dev Biol 52:585-94. 2008
    ..As yet we do not fully understand how the target of calcium in a mammalian egg might decode the patterns of calcium changes that can occur during egg activation...
  17. ncbi Reduced fertility of female mice lacking CD81
    Eric Rubinstein
    INSERM U602, Villejuif, France
    Dev Biol 290:351-8. 2006
    ..Finally, a fraction of CD9 was transferred from CD81-/- oocytes to sperm present in the perivitelline space indicating that the defect of fusion of CD81-/- oocytes does not result from an impaired initial gamete interaction...
  18. ncbi PLCzeta(zeta): a sperm protein that triggers Ca2+ oscillations and egg activation in mammals
    K Swann
    Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
    Semin Cell Dev Biol 17:264-73. 2006
    ..We review the evidence that PLCzeta represents the sperm factor that activates development of the egg and discuss the characteristics of PLCzeta that distinguish it from the somatic forms of PLC...
  19. ncbi Severely reduced female fertility in CD9-deficient mice
    F Le Naour
    Institut National de la Sante et de la Recherche Medicale INSERM, Unité 268, Hopital Paul Brousse, 94800 Villejuif, France
    Science 287:319-21. 2000
    ..Thus, CD9 appears to be essential for sperm-egg fusion, a process involving the CD9-associated integrin alpha6beta1...
  20. ncbi Biochemical and molecular characterization of bovine fertilin alpha and beta (ADAM 1 and ADAM 2): a candidate sperm-egg binding/fusion complex
    S I Waters
    Department of Cell Biology, University of Virginia, Charlottesville 22908, USA
    Biol Reprod 56:1245-54. 1997
    ..Gradient sedimentation experiments suggest that the fertilin alpha/beta heterodimer may be present on the sperm surface as a higher-order oligomer...
  21. ncbi Cytochalasin-D retards sperm incorporation deep into the egg cytoplasm but not membrane fusion with the egg plasma membrane
    Manuel Sánchez-Gutiérrez
    Departamento de Biologia Celular, Centro de Investigacón y de Estudios Avanzados del Instituto Politécnico Nacional, Apdo Postal 14 740, Mexico, DF
    Mol Reprod Dev 63:518-28. 2002
    ..These results show that actin polymerization is necessary for spermatozoa incorporation deep into the egg cytoplasm, but not for plasma membrane fusion nor egg activation early steps...
  22. ncbi Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distribution
    Kathryn E Runge
    Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
    Dev Biol 304:317-25. 2007
    ..They may also have a dynamic interaction with the sperm that serves to capture the sperm cell and bring it into close contact with the oocyte plasma membrane...
  23. ncbi Is vitronectin the velcro that binds the gametes together?
    F M Fusi
    Department of Obstetrics and Gynecology, Instituto Scientfico San Raffaele, Milano, Italy
    Mol Hum Reprod 2:859-66. 1996
    ..2 nM to 1 microM, but only in the presence of calcium ions. We propose that vitronectin released during the sperm acrosome reaction is recognized by both gametes and plays a role in their adhesion...
  24. ncbi Analysis of mouse fertilin in wild-type and fertilin beta(-/-) sperm: evidence for C-terminal modification, alpha/beta dimerization, and lack of essential role of fertilin alpha in sperm-egg fusion
    C Cho
    Section of Molecular and Cellular Biology, University of California at Davis, Davis, California, 95616, USA
    Dev Biol 222:289-95. 2000
    ..The results suggest instead that other gamete surface molecules act to promote membrane fusion and that fertilin's role in gamete fusion is in sperm-egg plasma membrane adhesion...
  25. ncbi Roles of the disintegrin domains of mouse fertilins alpha and beta in fertilization
    J P Evans
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
    Biol Reprod 59:145-52. 1998
    ....
  26. ncbi Mouse egg integrin alpha 6 beta 1 functions as a sperm receptor
    E A Almeida
    Department of Cell Biology, University of Virginia, Charlotteville 22908, USA
    Cell 81:1095-104. 1995
    ..Our results indicate a novel role for the integrin alpha 6 beta 1 as a cell-cell adhesion receptor that mediates sperm-egg binding...
  27. ncbi A potential fusion peptide and an integrin ligand domain in a protein active in sperm-egg fusion
    C P Blobel
    Department of Pharmacology, University of California San Francisco
    Nature 356:248-52. 1992
    ..Thus, the PH-30 alpha/beta complex resembles many viral fusion proteins in both its membrane topology and its predicted binding and fusion functions...
  28. pmc A role for the disintegrin domain of cyritestin, a sperm surface protein belonging to the ADAM family, in mouse sperm-egg plasma membrane adhesion and fusion
    R Yuan
    Molecular and Cellular Biology, University of California, Davis 95616, USA
    J Cell Biol 137:105-12. 1997
    ..Collectively, these data indicate that a second ADAM family member, cyritestin, functions with fertilin beta in sperm-egg plasma membrane adhesion leading to fusion...
  29. ncbi The C. elegans spe-9 gene encodes a sperm transmembrane protein that contains EGF-like repeats and is required for fertilization
    A Singson
    Department of Biology, Emory University, Atlanta, Georgia 30322, USA
    Cell 93:71-9. 1998
    ..These results suggest that SPE-9 functions in the specialized cell-cell interactions required for fertilization...
  30. ncbi Fertilization and inositol 1,4,5-trisphosphate (IP3)-induced calcium release in type-1 inositol 1,4,5-trisphosphate receptor down-regulated bovine eggs
    Christopher Malcuit
    Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, 01003, USA
    Biol Reprod 73:2-13. 2005
    ..Together, these results demonstrate the essential role of IP(3)R-1-mediated Ca(2+) release during fertilization in bovine eggs, and identify cell cycle regulatory mechanisms of [Ca(2+)](i) oscillations at the level of IP(3)R-1...
  31. pmc An Eph receptor sperm-sensing control mechanism for oocyte meiotic maturation in Caenorhabditis elegans
    Michael A Miller
    Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
    Genes Dev 17:187-200. 2003
    ..MSP-domain proteins are found in diverse animal taxa, where they may regulate contact-dependent Eph receptor signaling pathways...
  32. ncbi Cell adhesion and fertilization: steps in oocyte transport, sperm-zona pellucida interactions, and sperm-egg fusion
    Prudence Talbot
    Department of Neuroscience, University of California Riverside, Riverside, California 92521, USA
    Biol Reprod 68:1-9. 2003
    ..Last, we address the relationship between sperm adhesion to the egg plasma membrane and membrane fusion, especially the role of ADAM family proteins on the sperm surface and egg integrins...
  33. ncbi Calmodulin-dependent protein kinase II triggers mouse egg activation and embryo development in the absence of Ca2+ oscillations
    Jason G Knott
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia, PA 19104, USA
    Dev Biol 296:388-95. 2006
    ..These results strongly suggest that CaMKII is a major integrator of the Ca(2+) changes that occur following fertilization...
  34. ncbi ERp57 is a potential biomarker for human fertilization capability
    Jingjing Zhang
    Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, Jiangsu, PR China
    Mol Hum Reprod 13:633-9. 2007
    ..Furthermore, ERp57 could be a novel phenotype marker for male infertility and has the potential to be used to assess sperm selection for IVF...
  35. ncbi Development of calcium releasing activity induced by inositol trisphosphate and cyclic ADP-ribose during in vitro maturation of sea urchin oocytes
    Ken Miyata
    Department of Environmental Biology and Chemistry, Faculty of Science, University of Toyama, 3190 Gofuku, Toyama 930 8555, Japan
    Dev Growth Differ 48:605-13. 2006
    ..However, cADPR induced a small Ca(2+) transient even before meiosis, whereas IP(3) and sperm almost did not...
  36. ncbi Mammalian egg activation: from Ca2+ spiking to cell cycle progression
    Keith T Jones
    Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
    Reproduction 130:813-23. 2005
    ..APC/C activation leads to securin and cyclin B1 degradation and in so doing allows sister chromatids to be segregated and to decondense...
  37. ncbi Polypeptide backbone derived from carboxyl terminal of mouse ZP3 inhibits sperm-zona binding
    Ding Li
    Department of Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai, China
    Mol Reprod Dev 74:1327-36. 2007
    ..Our results suggest that the carboxyl terminal of mZP3 polypeptide backbone interacts with sperm and such interaction plays a significant role in sperm-zona binding, ultimately successful fertilization...
  38. ncbi The molecular basis of sperm-oocyte membrane interactions during mammalian fertilization
    Janice P Evans
    Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
    Hum Reprod Update 8:297-311. 2002
    ..The clinical relevance of correct gamete membrane interactions is also noted...
  39. ncbi A role for protein kinase C during rat egg activation
    Efrat Eliyahu
    Department of Embryology and Teratology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
    Biol Reprod 67:189-95. 2002
    ..We also demonstrate a successful inhibition of the CR by a myristoylated PKC pseudosubstrate (myrPKCPsi), a specific PKC inhibitor. Our study suggests that exocytosis can be triggered independently either by a [Ca(2+)](i) rise or by PKC...
  40. ncbi Multiple proteins present in purified porcine sperm apical plasma membranes interact with the zona pellucida of the oocyte
    Renske A van Gestel
    Department of Biochemistry and Cell Biology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
    Mol Hum Reprod 13:445-54. 2007
    ..Although we were not able to identify all of the proteins involved, this is a first step in understanding the event of primary sperm-zona interactions and the relevance of this for fertilization is discussed...
  41. ncbi Transfer of oocyte membrane fragments to fertilizing spermatozoa
    Virginie Barraud-Lange
    Universite Paris 13, UFR SMBH, Laboratoire de Biologie de la Reproduction, and AP HP Hôpital Jean Verdier, Bondy, France
    FASEB J 21:3446-9. 2007
    ..Acquisition of CD9 by the sperm may be crucial for the membrane reorganization in sperm required for fusion with the oocyte, a process that is similar to the role CD9 plays in oocyte membrane reorganization...
  42. ncbi Fertilin beta and other ADAMs as integrin ligands: insights into cell adhesion and fertilization
    J P Evans
    Department of Biochemistry and Molecular Biology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 615 N Wolfe St, Room 3606A, Baltimore, MD 21205, USA
    Bioessays 23:628-39. 2001
    ..Key issues in cell adhesion that pertain to gametes and fertilization will also be highlighted...
  43. ncbi Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion
    Dolores Busso
    Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina
    Biol Reprod 76:701-8. 2007
    ....
  44. ncbi Gonadotropin exposure, salt storage and storage duration affect penetration of domestic cat oocytes by homologous spermatozoa
    J R Herrick
    Center for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, 3400 Vine Street, OH 45220 1399, USA
    Theriogenology 59:1503-13. 2003
    ..05) in fresh IVM2 oocytes than in oocytes stored for 2-3 weeks or 2-3 months. These results suggest that oocyte penetration is improved by IVM, but is impaired by exposure to salt-storage solution and prolonged storage duration...
  45. ncbi Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarity
    Manqi Deng
    Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104 6018, USA
    Dev Biol 278:358-66. 2005
    ..These results provide new insights into how cortical reorganization occurs independently of extracellular signals to generate egg polarity...
  46. ncbi The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs
    Naokazu Inoue
    Genome Information Research Center, Osaka University, Yamadaoka 3 1, Suita, Osaka 565 0871, Japan
    Nature 434:234-8. 2005
    ..Human sperm also contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs...
  47. ncbi Sperm-egg fusion: events at the plasma membrane
    Kathryn K Stein
    Section of Molecular and Cellular Biology, School of Medicine, University of California Davis, Davis, CA 95616, USA
    J Cell Sci 117:6269-74. 2004
    ..These discoveries provide a strong starting point for the identification of additional proteins that have roles in sperm-egg fusion...
  48. ncbi Participation of the nonreducing terminal beta-galactosyl residues of the neutral N-linked carbohydrate chains of porcine zona pellucida glycoproteins in sperm-egg binding
    N Yonezawa
    Graduate School of Science and Technology, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba shi, Chiba 163 8522, Japan
    Mol Reprod Dev 70:222-7. 2005
    ..A correlation between the sperm-binding activity and in vitro fertilization rate is also presented...
  49. ncbi Zona pellucida binding ability and responsiveness to ionophore challenge of cryopreserved dog spermatozoa after different periods of capacitation in vitro
    A I Peña
    Unit of Reproduction and Obstetrics, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, Spain
    Anim Reprod Sci 84:193-210. 2004
    ....
  50. pmc Conventional PKCs regulate the temporal pattern of Ca2+ oscillations at fertilization in mouse eggs
    Guillaume Halet
    Department of Physiology, University College London, Gower Street, London WC1E 6BT, England, UK
    J Cell Biol 164:1033-44. 2004
    ..Finally, we present evidence that one physiological function of activated cPKCs in fertilized eggs is to sustain long-lasting Ca2+ oscillations, presumably via the regulation of store-operated Ca2+ entry...
  51. ncbi CD9 controls the formation of clusters that contain tetraspanins and the integrin alpha 6 beta 1, which are involved in human and mouse gamete fusion
    Ahmed Ziyyat
    Universite Paris 13, Laboratoire de Biologie de la Reproduction, UPRES 3410, UFR SMBH, Bobigny, France
    J Cell Sci 119:416-24. 2006
    ..Altogether, these data show that CD9 controls the redistribution of some membrane proteins including the alpha 6 beta1 integrin into clusters that may be necessary for gamete fusion...
  52. ncbi The mechanism of sperm-oocyte fusion in mammals
    Keisuke Kaji
    Institute for Stem Cell Research, The University of Edinburgh, Roger Land Building, The King s Building, West Mains Road, Edinburgh, EH9 3JQ, UK
    Reproduction 127:423-9. 2004
    ..In this review we highlight the candidate molecules involved in the sperm-oocyte interaction suggested from the recent progress in this research field...
  53. ncbi Reduced oocyte activation and first cleavage rate after ICSI with spermatozoa from a sterile mouse chromosome mutant
    E B Baart
    Laboratory of Genetics, Wageningen Institute of Animal Sciences, Wageningen University, 6701BH Wageningen, The Netherlands
    Hum Reprod 19:1140-7. 2004
    ..Male mice, heterozygous for two semi-identical reciprocal translocations T(1;13)70H and T(1;13)1Wa are usually sterile. We have investigated this oligoasthenoteratozoospermic mouse model using ICSI...
  54. ncbi Expression and structure-function analysis of de, a sperm cysteine-rich secretory protein that mediates gamete fusion
    Diego A Ellerman
    Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina
    Biol Reprod 67:1225-31. 2002
    ..To our knowledge, this is the first study reporting the relevance of structural components for the function of a CRISP member...
  55. ncbi Mammalian membrane block to polyspermy: new insights into how mammalian eggs prevent fertilisation by multiple sperm
    Allison J Gardner
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD 21205, USA
    Reprod Fertil Dev 18:53-61. 2006
    ....
  56. ncbi A short sperm-oocyte incubation time ZBA in the dog
    Ulrika Hermansson
    Department of Clinical Sciences, Division of Comparative Reproduction, Obstetrics and Udder Health, Swedish University of Agricultural Sciences, Box 7054, SE 750 07 Uppsala, Sweden
    Theriogenology 66:717-25. 2006
    ..Evaluating chilled and frozen-thawed dog spermatozoa using a zona pellucida binding assay...
  57. ncbi None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusion
    Zhi Yong He
    Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
    Dev Biol 254:226-37. 2003
    ....
  58. ncbi Sperm incorporation in Xenopus laevis: characterisation of morphological events and the role of microfilaments
    J A Boyle
    Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins 60614, USA
    Zygote 9:167-81. 2001
    ..Preincubation of eggs in jasplakinolide before latrunculin mitigated both these effects to some degree. However, eggs incubated in latrunculin either prior to or after insemination never progressed through first cleavage...
  59. ncbi Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin beta
    H Nishimura
    Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
    Dev Biol 233:204-13. 2001
    ..Because the altered sperm protein expression must be responsible for the fertilization defects, our data suggest new models for the molecular basis of the affected steps in fertilization...
  60. ncbi Fertilization defects in sperm from mice lacking fertilin beta
    C Cho
    Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
    Science 281:1857-9. 1998
    ..Fertilin could also have a direct role in sperm-zona binding or oviduct migration; alternatively, the effects on these functions could result from the absence of fertilin activity during spermatogenesis...
  61. ncbi Mouse sperm-egg plasma membrane interactions: analysis of roles of egg integrins and the mouse sperm homologue of PH-30 (fertilin) beta
    J P Evans
    Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
    J Cell Sci 108:3267-78. 1995
    ....
  62. ncbi Requirement of CD9 on the egg plasma membrane for fertilization
    K Miyado
    Institute of Life Science, Kurume University, Kurume, Fukuoka 839 0861, Japan
    Science 287:321-4. 2000
    ..When sperm were injected into oocytes with assisted microfertilization techniques, however, the fertilized eggs developed to term. These results indicate that CD9 has a crucial role in sperm-egg fusion...
  63. ncbi Male mice deficient for germ-cell cyritestin are infertile
    R Shamsadin
    Institute of Human Genetics, University of Gottingen, D 37073 Gottingen, Germany Institute of Genetics, University of Dusseldorf, D 40225 Dusseldorf, Germany
    Biol Reprod 61:1445-51. 1999
    ..These results demonstrate that cyritestin is crucial in the fertilization process at the level of the sperm-zona pellucida interaction...
  64. pmc Identification of a binding site in the disintegrin domain of fertilin required for sperm-egg fusion
    D G Myles
    Department of Physiology, University of Connecticut Health Center, Farmington 06030
    Proc Natl Acad Sci U S A 91:4195-8. 1994
    ..These results show that the disintegrin domain of fertilin beta binds to the egg plasma membrane and that this binding is required for membrane fusion...
  65. pmc The precursor region of a protein active in sperm-egg fusion contains a metalloprotease and a disintegrin domain: structural, functional, and evolutionary implications
    T G Wolfsberg
    Department of Pharmacology, University of California, San Francisco 94143
    Proc Natl Acad Sci U S A 90:10783-7. 1993
    ..PH-30, acting through its metalloprotease and/or disintegrin domains, could be involved in sperm development as well as sperm-egg binding and fusion. Phylogenetic analysis indicates that PH-30 stems from a multidomain ancestral protein...
  66. ncbi Characterization of the binding of recombinant mouse sperm fertilin beta subunit to mouse eggs: evidence for adhesive activity via an egg beta1 integrin-mediated interaction
    J P Evans
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
    Dev Biol 187:79-93. 1997
    ..These results suggest that a beta1-containing integrin participates in the binding of recombinant fertilin beta-EC to mouse eggs...
  67. ncbi Characterization of the binding of recombinant mouse sperm fertilin alpha subunit to mouse eggs: evidence for function as a cell adhesion molecule in sperm-egg binding
    J P Evans
    Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
    Dev Biol 187:94-106. 1997
    ..These data are the first evidence to suggest that fertilin alpha can function as a cell adhesion molecule during fertilization, mediating the binding of sperm and egg plasma membranes...
  68. ncbi Sperm from beta 1,4-galactosyltransferase-null mice are refractory to ZP3-induced acrosome reactions and penetrate the zona pellucida poorly
    Q Lu
    Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30302, USA
    Development 124:4121-31. 1997
    ....
  69. ncbi Localization of carbohydrate chains of pig sperm ligand in the glycoprotein ZPB of egg zona pellucida
    K Kudo
    Graduate School of Science and Technology, Chiba University, Japan
    Eur J Biochem 252:492-9. 1998
    ..These results suggest that the carbohydrate chains linked to Asn220 of ZPB participate predominantly in sperm-egg binding...
  70. ncbi Molecular cloning and characterization of P47, a novel boar sperm-associated zona pellucida-binding protein homologous to a family of mammalian secretory proteins
    M Ensslin
    Institut für Reproduktionsmedizin, Tierarztliche Hochschule, Hannover, Germany
    Biol Reprod 58:1057-64. 1998
    ..Northern blot analysis showed that P47 mRNA is transcribed in several porcine and bovine tissues. These data indicate a potential role for boar sperm-associated P47 in membrane remodeling and/or as a zona pellucida binding protein...
  71. ncbi Is the oocyte a non-professional phagocyte?
    R Bronson
    Department of Obstetrics and Gynecology, Health Sciences Center, State University of New York, New York 11794 8091, USA
    Hum Reprod Update 4:763-75. 1998
    ..It asks whether the oocyte might not utilize similar mechanisms during its incorporation of the spermatozoon...
  72. ncbi Sperm disintegrins, egg integrins, and other cell adhesion molecules of mammalian gamete plasma membrane interactions
    J P Evans
    Division of Reproductive Biology, Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD, USA
    Front Biosci 4:D114-31. 1999
    ....
  73. ncbi Can we use in vitro fertilization tests to predict semen fertility?
    B Larsson
    Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Centre of Reproduction in Uppsala CRU, Swedish University of Agricultural Sciences SLU, Uppsala, Sweden
    Anim Reprod Sci 60:327-36. 2000
    ..Used either as single tests or in combination with other tests, this type of assay can provide valuable information about a semen donor, an insemination dose or a method of semen preservation...
  74. ncbi Increasing uterine receptivity by decreasing estradiol levels during the preimplantation period in high responders with the use of a follicle-stimulating hormone step-down regimen
    C Simon
    Instituto Valenciano de Infertilidad and Department of Pediatrics, Obstetrics and Gynecology, Valencia University, Spain
    Fertil Steril 70:234-9. 1998
    ..To analyze the effect on uterine receptivity of a decrease in E2 levels during the preimplantation period with the use of a step-down regimen in high responders undergoing IVF...
  75. ncbi Role for sperm in spatial patterning of the early mouse embryo
    K Piotrowska
    Wellcome/CRC Institute and Department of Genetics, University of Cambridge, UK
    Nature 409:517-21. 2001
    ..We present a model for axial development that accommodates these findings with the regulative nature of mouse embryos...
  76. ncbi Analysis of Mn(2+)/Ca(2+) influx and release during Ca(2+) oscillations in mouse eggs injected with sperm extract
    T Mohri
    Laboratory of Intracellular Metabolism, Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan
    Cell Calcium 29:311-25. 2001
    ..The finding that repetitive Mn(2+) release is caused by continuous Mn(2+) entry suggests that continuous Ca(2+) influx may play a critical role in refilling the ER and, thereby, maintaining Ca(2+)oscillations in mammalian fertilization...
  77. ncbi Subcellular localization of beta1,4-galactosyltransferase on bull sperm and its function during sperm-egg interactions
    M W Tengowski
    Wisconsin Regional Primate Research Center and Department of Zoology, University of Wisconsin, Madison, Wisconsin, USA
    Mol Reprod Dev 58:236-44. 2001
    ..The function of GalT in both the murine and bovine systems suggests that it may serve as a generalized gamete receptor in mammals...
  78. ncbi Mammalian fertilization: molecular aspects of gamete adhesion, exocytosis, and fusion
    P M Wassarman
    Department of Cell Biology, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
    Cell 96:175-83. 1999
  79. pmc Sperm mitochondrial integrity is not required for hyperactivated motility, zona binding, or acrosome reaction in the rhesus macaque
    Pei Hsuan Hung
    Molecular, Cellular, and Integrative Physiology, University of California, Davis, Davis, California 95616, USA
    Biol Reprod 79:367-75. 2008
    ....
  80. ncbi Hyper-osmotic condition enhances protein tyrosine phosphorylation and zona pellucida binding capacity of human sperm
    D Y Liu
    Department of Obstetrics and Gynecology, University of Melbourne, Reproductive Services and Andrology Laboratory, Royal Women s Hospital and Melbourne IVF, Melbourne, Australia
    Hum Reprod 21:745-52. 2006
    ....
  81. ncbi Atrial natriuretic peptide induces an acrosome reaction in giant panda spermatozoa and enhances their penetration of salt-stored porcine oocytes
    Meijia Zhang
    Department of Animal Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, PR China
    Theriogenology 64:1297-308. 2005
    ..Furthermore, ANP enhanced the penetrability of porcine salt-stored oocytes by frozen-thawed giant panda spermatozoa...
  82. ncbi In vitro penetration of fresh and vitrified swine oocytes by homologous spermatozoa using different incubation systems
    Milton C Macedo
    Faculdade de Veterinaria, Centro de Biotecnologia, Universidade Federal de Pelotas, 96010 900 Pelotas RS, Brazil
    Anim Reprod Sci 92:334-48. 2006
    ..The IVP test was the only method of semen evaluation that identified the reduction in semen quality up to 72 h of storage...
  83. ncbi Reassessing the role of progesterone in fertilization--compartmentalized calcium signalling in human spermatozoa?
    Claire V Harper
    School of Biosciences, University of Birmingham, UK
    Hum Reprod 20:2675-80. 2005
    ....
  84. ncbi The clinical significance of sperm-zona pellucida binding: 17 years later
    Daniel R Franken
    Department of Obstetrics and Gynaecology, University of Stellenbosch, Tygerberg, South Africa
    Front Biosci 11:1227-33. 2006
    ..We enter the next millennium with many questions that remain to be answered by the hand of efficacious screening techniques and a new formidable therapy in intracytoplasmic sperm injection...
  85. ncbi Effects of plasmin on sperm-oocyte interactions during in vitro fertilization in the pig
    S J Sa
    Division of Animal Resource Science, College of Animal Resource Science, Kangwon University, Chunchon 200 701, South Korea
    Anim Reprod Sci 95:273-82. 2006
    ..These results suggest that plasmin might play a role in events related to fertilization...
  86. doi RANTES and human sperm fertilizing ability: effect on acrosome reaction and sperm/oocyte fusion
    A Barbonetti
    Andrologic Unit, Department of Internal Medicine, University of L Aquila, Blocco 11, Coppito 67100, Coppito, L Aquila, Italy
    Mol Hum Reprod 14:387-91. 2008
    ....
  87. ncbi Human sperm do not bind to rat zonae pellucidae despite the presence of four homologous glycoproteins
    Tanya Hoodbhoy
    Laboratory of Cellular and Developmental Biology, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Biol Chem 280:12721-31. 2005
    ..These data suggest that the presence of four zona proteins are not sufficient to support human sperm binding to rodent eggs and that additional determinants must be responsible for taxon-specific fertilization among mammals...
  88. pmc Cytogenetic analysis of sperm nucleous components of iranian normal and sub-fertile individuals using zona free hamster oocytes
    H Mozdarani
    Department of Infertility Genetics, Royan Institute, Tehran, Iran
    J Assist Reprod Genet 21:409-14. 2004
    ..PCC rate was studied for evaluating the role of sperm chromatin abnormalities in the process of nuclear decondensation...
  89. doi Sperm membrane physiology and relevance for fertilization
    B M Gadella
    Utrecht University, Faculty of Veterinary Medicine, Departments of Biochemistry and Cell Biology and of Farm Animal Health, Utrecht, The Netherlands
    Anim Reprod Sci 107:229-36. 2008
    ..This paper emphasizes biochemical models for membrane surface modelling and their potential involvement for remodelling the sperm surface in the above described processes...
  90. ncbi Production of normal offspring from partially zona-incised vitrified mouse oocytes fertilized with cryopreserved spermatozoa using an optimized protocol
    Yan Ping Wang
    Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing, China
    Cryo Letters 29:111-9. 2008
    ....
  91. ncbi Exposure to trichloroethylene and its metabolites causes impairment of sperm fertilizing ability in mice
    Hongbin Xu
    Ottawa Health Research Institute, Hormones, Growth, Development, and Department of Biochemistry Microbiology Immunology, University of Ottawa, Ottawa, Ontario, Canada K1Y 4E9
    Toxicol Sci 82:590-7. 2004
    ..1-10 microg/mL), although the decreases were not as pronounced as those for CH. These results showed that TCE exposure leads to impairment of sperm fertilizing ability, which may be attributed to TCE metabolites, CH, and TCOH...
  92. ncbi Proteasomal interference prevents zona pellucida penetration and fertilization in mammals
    Peter Sutovsky
    Department of Animal Science, University of Missouri Columbia, S141 ASRC, 920 East Campus Dr, Columbia, MO 65211 5300, USA
    Biol Reprod 71:1625-37. 2004
    ....
  93. ncbi In vitro evaluation of the quality and fertilizing capacity of boar semen frozen in 0.25 ml straws
    J Pelaez
    Department of Animal Pathology Animal Health, University of Leon, Leon, Spain
    Reprod Domest Anim 41:153-61. 2006
    ..Moreover, the approach of selecting suitable protocols of cryopreservation does not appear to be sufficient for guaranteeing systematically good quality and fertilizing capacity in the frozen-thawed semen...
  94. doi Viability, acrosome morphology and fertilizing capacity of boar spermatozoa treated with strontium chloride
    Konosuke Okada
    Department of Animal Science, Nippon Veterinary and Life Science University, 1 7 1 Kyonan cho, Musashino, Tokyo 180 0023, Japan
    Zygote 16:49-56. 2008
    ..These results demonstrate that Sr2+ is likely to positively affect the fertilizing capacity of spermatozoa in the pig...
  95. ncbi New insights into the molecular mechanisms of sperm-egg interaction
    B Nixon
    Reproductive Science Group, ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
    Cell Mol Life Sci 64:1805-23. 2007
    ..Herein, we describe two models indicating the plethora of highly orchestrated molecular interactions underlying successful sperm zona binding and sperm oocyte fusion...
  96. ncbi D-Mannose-binding sites are putative sperm determinants of human oocyte recognition and fertilization
    Germán Rosano
    Laboratorio de Estudios Reproductivos, Cátedra de Bioquímica Clínica, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, San Lorenzo 939 9A 2000, Rosario, Argentina
    Reprod Biomed Online 15:182-90. 2007
    ..The data show that D-mannose is involved in the sperm-zona pellucida interaction, and that the expression of MBS on the sperm surface occurs during the acquisition of in-vitro sperm fertilizing ability...
  97. ncbi Distribution of alpha-D-mannose residues on zona pellucida and their role(s) in fertilization in pigs
    XueXiong Song
    Faculty of Animal Science and Technology, Laiyang Agricultural College, Qingdao 266109, China
    Sci China C Life Sci 50:170-7. 2007
    ..These results suggest that alpha-D-mannose residues presenting on pig ZP might be an important component of sperm receptor and might induce sperm acrosome reaction and thus facilitate the sperm penetration into the ZP...
  98. ncbi Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semen
    De Yi Liu
    Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women s Hospital, 132, Grattan Street, Carlton, Victoria 3053, Australia
    Hum Reprod 22:1878-84. 2007
    ..e. male partners of infertile couples) with normal and abnormal semen analyses...
  99. ncbi CaMKII can participate in but is not sufficient for the establishment of the membrane block to polyspermy in mouse eggs
    Allison J Gardner
    Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
    J Cell Physiol 212:275-80. 2007
    ....
  100. pmc Egg water from the amphibian Bufo arenarum induces capacitation-like changes in homologous spermatozoa
    Dario Krapf
    División Biología del Desarrollo, IBR CONICET UNR and Area Biología, FCByF, UNR, Argentina
    Dev Biol 306:516-24. 2007
    ..These data indicate that sperm should undergo a series of molecular changes to gain fertilizing capacity; these changes are reminiscent of mammalian sperm capacitation and take place before the acrosome reaction...
  101. ncbi Evaluation of sperm functional attributes in relation to in vitro sperm-zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen quality
    S Selvaraju
    Reproductive Physiology Laboratory, Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
    Anim Reprod Sci 106:311-21. 2008
    ..70) and plasmalemma integrity measured by HOS-G test (r=0.68). The present study indicates that these attributes could represent important determinants of buffalo sperm quality influencing cleavage rate...

Research Grants65

  1. Gamete membrane adhesion and fusion during fertilization
    William J Snell; Fiscal Year: 2010
    ....
  2. EPIDEMIOLOGIC AND BIOLOGIC PREDICTORS OF IVF SUCCESS
    Daniel Cramer; Fiscal Year: 2002
    ....
  3. GAMETE MEMBRANE ADHESINO AND FUSION DURING FERTILIZATION
    William Snell; Fiscal Year: 2009
    ..abstract_text> ..
  4. GAMETE MEMBRANE ADHESINO AND FUSION DURING FERTILIZATION
    William Snell; Fiscal Year: 2007
    ....
  5. SPERM FUNCTION IN FERTILIZATION EVENTS
    Gary Olson; Fiscal Year: 1993
    ....
  6. Gamete membrane adhesion and fusion during fertilization
    William Snell; Fiscal Year: 2006
    ..Currently, the molecular mechanisms of gamete fusion are not understood in any organism. Understanding gamete fusion in Chlamydomonas should inform future studies on human reproduction. ..
  7. Fertilization and Activation of Development 2007 Gordon Research Conference
    GEORGE GERTON; Fiscal Year: 2007
    ..The conference will promote collaborations among US and international scientists with widely disparate experiences/seniority, methodologies, and model systems. ..
  8. ROLE OF MAMMALIAN SPERM SURFACE MANNOSIDASE
    DAULAT TULSIANI; Fiscal Year: 1992
    ....
  9. Gordon Conference/Fertilization/Activation of Developm
    RICHARD CARDULLO; Fiscal Year: 2003
    ..These latter talks will particularly feature junior scientists, including postdoctoral fellows and students. In addition, we will have a keynote speaker for the Tuesday evening session. ..
  10. FUNCTIONS OF PROTEIN DOMAINS IN GAMETE INTERACTIONS
    Daniel Hardy; Fiscal Year: 2002
    ..By adding to general knowledge of cell-cell interactions, they may also lead to a better understanding of other medical conditions such as development problems, cancer, blood disorders, and neurological diseases. ..
  11. SPAM 1 ALLELES, SPERM PHENOTYPE AND FUNCTION
    Patricia DeLeon; Fiscal Year: 2004
    ....
  12. Recombinant Antibodies as Intra-Vaginal Spermicides
    Michael Coppola; Fiscal Year: 2002
    ..Finally, the stability and safety of the optimized formulation will be assessed. PROPOSED COMMERCIAL APPLICATION: Topical intra-vaginal contraceptive. ..