Genomes and Genes
sperm ovum interactions
Summary: Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.
Publications205 found, 100 shown here
- Platelet activating factor improves the in vitro penetration of zona free hamster eggs by buffalo (Bubalus bubalis) spermatozoaSubodh Kumar
Biotechnology Laboratory, National Dairy Research Institute, Karnal 132 001, India
Theriogenology 63:1564-72. 2005..18, (14.90); 0.79 +/- 0.02, 1.10 +/- 0.03, (0.31) and 5.22 +/- 1.22, 21.69 +/- 1.88, (16.47)%, respectively. In conclusion, PAF significantly increased the AR and other aspects of fertilization, despite a small reduction in motility...
- Severely reduced female fertility in CD9-deficient miceF Le Naour
Institut National de la Sante et de la Recherche Medicale INSERM, Unité 268, Hopital Paul Brousse, 94800 Villejuif, France
Science 287:319-21. 2000..Thus, CD9 appears to be essential for sperm-egg fusion, a process involving the CD9-associated integrin alpha6beta1...
- Requirement of CD9 on the egg plasma membrane for fertilizationK Miyado
Institute of Life Science, Kurume University, Kurume, Fukuoka 839 0861, Japan
Science 287:321-4. 2000..When sperm were injected into oocytes with assisted microfertilization techniques, however, the fertilized eggs developed to term. These results indicate that CD9 has a crucial role in sperm-egg fusion...
- The gamete fusion process is defective in eggs of Cd9-deficient miceK Kaji
Department of Life Science, Tokyo Institute of Technology, Yokohama, Japan
Nat Genet 24:279-82. 2000..In normal animals, Cd9 molecules were expressed on the egg microvilli and became densely concentrated at the sperm attachment site. Thus, our results show that Cd9 is important in the gamete fusion process at fertilization...
- Fertilization defects in sperm from mice lacking fertilin betaC Cho
Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA
Science 281:1857-9. 1998..Fertilin could also have a direct role in sperm-zona binding or oviduct migration; alternatively, the effects on these functions could result from the absence of fertilin activity during spermatogenesis...
- The dynamics of calcium oscillations that activate mammalian eggsKarl Swann
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Cardiff, UK
Int J Dev Biol 52:585-94. 2008..As yet we do not fully understand how the target of calcium in a mammalian egg might decode the patterns of calcium changes that can occur during egg activation...
- PLCzeta(zeta): a sperm protein that triggers Ca2+ oscillations and egg activation in mammalsK Swann
Department of Obstetrics and Gynaecology, School of Medicine, Cardiff University, Heath Park, Cardiff CF14 4XN, UK
Semin Cell Dev Biol 17:264-73. 2006..We review the evidence that PLCzeta represents the sperm factor that activates development of the egg and discuss the characteristics of PLCzeta that distinguish it from the somatic forms of PLC...
- Analysis of loss of adhesive function in sperm lacking cyritestin or fertilin betaH Nishimura
Section of Molecular and Cellular Biology, University of California, Davis, California 95616, USA
Dev Biol 233:204-13. 2001..Because the altered sperm protein expression must be responsible for the fertilization defects, our data suggest new models for the molecular basis of the affected steps in fertilization...
- Reduced fertility of female mice lacking CD81Eric Rubinstein
INSERM U602, Villejuif, France
Dev Biol 290:351-8. 2006..Finally, a fraction of CD9 was transferred from CD81-/- oocytes to sperm present in the perivitelline space indicating that the defect of fusion of CD81-/- oocytes does not result from an impaired initial gamete interaction...
- Mouse egg integrin alpha 6 beta 1 functions as a sperm receptorE A Almeida
Department of Cell Biology, University of Virginia, Charlotteville 22908, USA
Cell 81:1095-104. 1995..Our results indicate a novel role for the integrin alpha 6 beta 1 as a cell-cell adhesion receptor that mediates sperm-egg binding...
- None of the integrins known to be present on the mouse egg or to be ADAM receptors are essential for sperm-egg binding and fusionZhi Yong He
Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
Dev Biol 254:226-37. 2003....
- Sperm phospholipase Czeta from humans and cynomolgus monkeys triggers Ca2+ oscillations, activation and development of mouse oocytesL J Cox
Cell Signalling Laboratory, Wales Heart Research Institute, University of Wales College of Medicine, Cardiff CF14 4XN, UK
Reproduction 124:611-23. 2002..These results are consistent with the proposal that sperm PLCzeta is the molecular trigger for egg activation during fertilization and that the role and activity of PLCzeta is highly conserved across mammalian species...
- Spatiotemporal analysis of Ca(2+) waves in relation to the sperm entry site and animal-vegetal axis during Ca(2+) oscillations in fertilized mouse eggsR Deguchi
Laboratory of Intracellular Metabolism, Department of Molecular Physiology, National Institute for Physiological Sciences, Myodaiji cho, Okazaki, 444 8585, Japan
Dev Biol 218:299-313. 2000..Since the shift of the wave initiation site to the vegetal cortex is observed in fertilized eggs of nemertean worms and ascidians, this might be an evolutionarily conserved feature...
- A role for the disintegrin domain of cyritestin, a sperm surface protein belonging to the ADAM family, in mouse sperm-egg plasma membrane adhesion and fusionR Yuan
Molecular and Cellular Biology, University of California, Davis 95616, USA
J Cell Biol 137:105-12. 1997..Collectively, these data indicate that a second ADAM family member, cyritestin, functions with fertilin beta in sperm-egg plasma membrane adhesion leading to fusion...
- Insights into the molecular basis of sperm-egg recognition in mammalsTanya Hoodbhoy
Laboratory of Cellular and Developmental Biology, NIDDK, Building 50, Room 3128, National Institutes of Health, 50 South Drive, MSC 8028, Bethesda, Maryland 20892, USA
Reproduction 127:417-22. 2004..Taken together, these data support a model in which the cleavage status of ZP2 modulates the three-dimensional structure of the zona pellucida and determines whether sperm bind (uncleaved) or do not (cleaved)...
- A role for protein kinase C during rat egg activationEfrat Eliyahu
Department of Embryology and Teratology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
Biol Reprod 67:189-95. 2002..We also demonstrate a successful inhibition of the CR by a myristoylated PKC pseudosubstrate (myrPKCPsi), a specific PKC inhibitor. Our study suggests that exocytosis can be triggered independently either by a [Ca(2+)](i) rise or by PKC...
- Sperm from beta 1,4-galactosyltransferase-null mice are refractory to ZP3-induced acrosome reactions and penetrate the zona pellucida poorlyQ Lu
Department of Cell Biology, Emory University School of Medicine, Atlanta, GA 30302, USA
Development 124:4121-31. 1997....
- Male mice deficient for germ-cell cyritestin are infertileR Shamsadin
Institute of Human Genetics, University of Gottingen, D 37073 Gottingen, Germany Institute of Genetics, University of Dusseldorf, D 40225 Dusseldorf, Germany
Biol Reprod 61:1445-51. 1999..These results demonstrate that cyritestin is crucial in the fertilization process at the level of the sperm-zona pellucida interaction...
- Analysis of Mn(2+)/Ca(2+) influx and release during Ca(2+) oscillations in mouse eggs injected with sperm extractT Mohri
Laboratory of Intracellular Metabolism, Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan
Cell Calcium 29:311-25. 2001..The finding that repetitive Mn(2+) release is caused by continuous Mn(2+) entry suggests that continuous Ca(2+) influx may play a critical role in refilling the ER and, thereby, maintaining Ca(2+)oscillations in mammalian fertilization...
- Analysis of mouse fertilin in wild-type and fertilin beta(-/-) sperm: evidence for C-terminal modification, alpha/beta dimerization, and lack of essential role of fertilin alpha in sperm-egg fusionC Cho
Section of Molecular and Cellular Biology, University of California at Davis, Davis, California, 95616, USA
Dev Biol 222:289-95. 2000..The results suggest instead that other gamete surface molecules act to promote membrane fusion and that fertilin's role in gamete fusion is in sperm-egg plasma membrane adhesion...
- Cell adhesion and fertilization: steps in oocyte transport, sperm-zona pellucida interactions, and sperm-egg fusionPrudence Talbot
Department of Neuroscience, University of California Riverside, Riverside, California 92521, USA
Biol Reprod 68:1-9. 2003..Last, we address the relationship between sperm adhesion to the egg plasma membrane and membrane fusion, especially the role of ADAM family proteins on the sperm surface and egg integrins...
- Molecular cloning and characterization of rat sperm surface antigen 2B1, a glycoprotein implicated in sperm-zona bindingS T Hou
Department of Biochemistry, School of Medical Sciences, University of Bristol, UK
Mol Reprod Dev 45:193-203. 1996....
- Are Src family kinases involved in cell cycle resumption in rat eggs?A Talmor-Cohen
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv 69978, Tel Aviv, Israel
Reproduction 127:455-63. 2004..The possible involvement of SFKs in the signal transduction pathways that lead from the sperm-egg fusion site downstream of the Ca2+ release remains unclear...
- Testis-specific antigen (TSA-1) is expressed in murine sperm and its antibodies inhibit fertilizationRam N Trivedi
Department of Obstetrics and Gynecology, Medical College of Ohio, Toledo 43614 5806, USA
Am J Reprod Immunol 47:38-45. 2002..The present study was conducted to examine its expression and function in murine sperm, in order to find out whether or not the mouse can provide a suitable model for examining its immunocontraceptive effects...
- Evidence for distinct serine protease activities with a potential role in processing the sperm protein fertilinL Lum
Program in Cellular Biochemistry and Biophysics, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA
Dev Biol 191:131-45. 1997....
- Cyclic FEE peptide increases human gamete fusion and potentiates its RGD-induced inhibitionA Ziyyat
Laboratoire de Biologie de la Reproduction, UPRES 3410, UFR SMBH, Universite Paris 13, 74, rue Marcel Cachin, 93017 Bobigny, Service d Histologie Embryologie Cytogénétique, Hôpital Jean Verdier Assistance Publique Hôpitaux de Paris, Bondy, France
Hum Reprod 20:3452-8. 2005..We investigated, in humans, whether oocyte integrins could act similarly in gamete fusion, using a cyclic peptide containing the putative disintegrin-binding domain of human fertilin beta [cyclic FEE (cFEE)] and RGD peptide...
- Mediation of sperm-egg fusion: evidence that mouse egg alpha6beta1 integrin is the receptor for sperm fertilinbetaH Chen
Department of Chemistry, State University of New York, Stony Brook 11794 3400, USA
Chem Biol 6:1-10. 1999..We set out to identify the fertilinbeta binding partner on the egg plasma membrane...
- Contribution of mouse egg zona pellucida glycoproteins to gamete recognition during fertilizationPaul M Wassarman
Brookdale Department of Molecular, Cell and Developmental Biology, Mount Sinai School of Medicine, One Gustave L Levy Place, New York, New York, USA
J Cell Physiol 204:388-91. 2005....
- SAMP32, a testis-specific, isoantigenic sperm acrosomal membrane-associated proteinZhonglin Hao
Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, Virginia 22908, USA
Biol Reprod 66:735-44. 2002..Recombinant SAMP32 reacted with serum from an infertile man, suggesting that it is isoantigenic. Antibodies against recombinant SAMP32 inhibited both the binding and the fusion of human sperm to zona-free hamster eggs...
- Mammalian fertilization: molecular aspects of gamete adhesion, exocytosis, and fusionP M Wassarman
Department of Cell Biology, Mount Sinai School of Medicine, New York, New York 10029 6574, USA
Cell 96:175-83. 1999
- Egg activation at fertilization: where it all beginsLinda L Runft
Department of Physiology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA
Dev Biol 245:237-54. 2002....
- A role for sperm surface protein disulfide isomerase activity in gamete fusion: evidence for the participation of ERp57Diego A Ellerman
Department of Cell Biology and Human Anatomy, School of Medicine, University of California, Davis, Davis, California 95616, USA
Dev Cell 10:831-7. 2006..The results support the idea that thiol-disulfide exchange is a mechanism that may act in gamete fusion to produce conformational changes in fusion-active proteins...
- Identification of mouse sperm SED1, a bimotif EGF repeat and discoidin-domain protein involved in sperm-egg bindingMichael A Ensslin
Department of Cell Biology, Whitehead Biomedical Research Building, Emory University School of Medicine, 615 Michael Street, Room 405, Atlanta, GA 30322, USA
Cell 114:405-17. 2003..These studies illustrate that Notch-like EGF and discoidin/C domains, protein motifs that facilitate a variety of cellular interactions, participate in gamete recognition as well...
- SAMP14, a novel, acrosomal membrane-associated, glycosylphosphatidylinositol-anchored member of the Ly-6/urokinase-type plasminogen activator receptor superfamily with a role in sperm-egg interactionJagathpala Shetty
Department of Cell Biology, Center for Research in Contraceptive and Reproductive Health, University of Virginia, Charlottesville, Virginia 22908, USA
J Biol Chem 278:30506-15. 2003..SAMP14 represents a GPI-anchored putative receptor in the Ly-6/uPAR family that is exposed on the inner acrosomal membrane after the acrosome reaction...
- Oocyte CD9 is enriched on the microvillar membrane and required for normal microvillar shape and distributionKathryn E Runge
Section of Molecular and Cellular Biology, University of California Davis, Davis, CA 95616, USA
Dev Biol 304:317-25. 2007..They may also have a dynamic interaction with the sperm that serves to capture the sperm cell and bring it into close contact with the oocyte plasma membrane...
- Biochemical and molecular characterization of bovine fertilin alpha and beta (ADAM 1 and ADAM 2): a candidate sperm-egg binding/fusion complexS I Waters
Department of Cell Biology, University of Virginia, Charlottesville 22908, USA
Biol Reprod 56:1245-54. 1997..Gradient sedimentation experiments suggest that the fertilin alpha/beta heterodimer may be present on the sperm surface as a higher-order oligomer...
- Is vitronectin the velcro that binds the gametes together?F M Fusi
Department of Obstetrics and Gynecology, Instituto Scientfico San Raffaele, Milano, Italy
Mol Hum Reprod 2:859-66. 1996..2 nM to 1 microM, but only in the presence of calcium ions. We propose that vitronectin released during the sperm acrosome reaction is recognized by both gametes and plays a role in their adhesion...
- Roles of the disintegrin domains of mouse fertilins alpha and beta in fertilizationJ P Evans
Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
Biol Reprod 59:145-52. 1998....
- A potential fusion peptide and an integrin ligand domain in a protein active in sperm-egg fusionC P Blobel
Department of Pharmacology, University of California San Francisco
Nature 356:248-52. 1992..Thus, the PH-30 alpha/beta complex resembles many viral fusion proteins in both its membrane topology and its predicted binding and fusion functions...
- The molecular basis of sperm-oocyte membrane interactions during mammalian fertilizationJanice P Evans
Department of Biochemistry and Molecular Biology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA
Hum Reprod Update 8:297-311. 2002..The clinical relevance of correct gamete membrane interactions is also noted...
- ERp57 is a potential biomarker for human fertilization capabilityJingjing Zhang
Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University, Nanjing 210029, Jiangsu, PR China
Mol Hum Reprod 13:633-9. 2007..Furthermore, ERp57 could be a novel phenotype marker for male infertility and has the potential to be used to assess sperm selection for IVF...
- Mammalian egg activation: from Ca2+ spiking to cell cycle progressionKeith T Jones
Institute for Cell and Molecular Biosciences, The Medical School, Framlington Place, University of Newcastle, Newcastle, NE2 4HH, UK
Reproduction 130:813-23. 2005..APC/C activation leads to securin and cyclin B1 degradation and in so doing allows sister chromatids to be segregated and to decondense...
- Molecular cloning and characterization of P47, a novel boar sperm-associated zona pellucida-binding protein homologous to a family of mammalian secretory proteinsM Ensslin
Institut für Reproduktionsmedizin, Tierarztliche Hochschule, Hannover, Germany
Biol Reprod 58:1057-64. 1998..Northern blot analysis showed that P47 mRNA is transcribed in several porcine and bovine tissues. These data indicate a potential role for boar sperm-associated P47 in membrane remodeling and/or as a zona pellucida binding protein...
- Localization of carbohydrate chains of pig sperm ligand in the glycoprotein ZPB of egg zona pellucidaK Kudo
Graduate School of Science and Technology, Chiba University, Japan
Eur J Biochem 252:492-9. 1998..These results suggest that the carbohydrate chains linked to Asn220 of ZPB participate predominantly in sperm-egg binding...
- Fertilin beta and other ADAMs as integrin ligands: insights into cell adhesion and fertilizationJ P Evans
Department of Biochemistry and Molecular Biology, Johns Hopkins University School of Hygiene and Public Health, Baltimore, MD 615 N Wolfe St, Room 3606A, Baltimore, MD 21205, USA
Bioessays 23:628-39. 2001..Key issues in cell adhesion that pertain to gametes and fertilization will also be highlighted...
- Identification of a binding site in the disintegrin domain of fertilin required for sperm-egg fusionD G Myles
Department of Physiology, University of Connecticut Health Center, Farmington 06030
Proc Natl Acad Sci U S A 91:4195-8. 1994..These results show that the disintegrin domain of fertilin beta binds to the egg plasma membrane and that this binding is required for membrane fusion...
- CD9 controls the formation of clusters that contain tetraspanins and the integrin alpha 6 beta 1, which are involved in human and mouse gamete fusionAhmed Ziyyat
Universite Paris 13, Laboratoire de Biologie de la Reproduction, UPRES 3410, UFR SMBH, Bobigny, France
J Cell Sci 119:416-24. 2006..Altogether, these data show that CD9 controls the redistribution of some membrane proteins including the alpha 6 beta1 integrin into clusters that may be necessary for gamete fusion...
- The precursor region of a protein active in sperm-egg fusion contains a metalloprotease and a disintegrin domain: structural, functional, and evolutionary implicationsT G Wolfsberg
Department of Pharmacology, University of California, San Francisco 94143
Proc Natl Acad Sci U S A 90:10783-7. 1993..PH-30, acting through its metalloprotease and/or disintegrin domains, could be involved in sperm development as well as sperm-egg binding and fusion. Phylogenetic analysis indicates that PH-30 stems from a multidomain ancestral protein...
- Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusionDolores Busso
Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina
Biol Reprod 76:701-8. 2007....
- Development of calcium releasing activity induced by inositol trisphosphate and cyclic ADP-ribose during in vitro maturation of sea urchin oocytesKen Miyata
Department of Environmental Biology and Chemistry, Faculty of Science, University of Toyama, 3190 Gofuku, Toyama 930 8555, Japan
Dev Growth Differ 48:605-13. 2006..However, cADPR induced a small Ca(2+) transient even before meiosis, whereas IP(3) and sperm almost did not...
- An Eph receptor sperm-sensing control mechanism for oocyte meiotic maturation in Caenorhabditis elegansMichael A Miller
Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232, USA
Genes Dev 17:187-200. 2003..MSP-domain proteins are found in diverse animal taxa, where they may regulate contact-dependent Eph receptor signaling pathways...
- Mammalian membrane block to polyspermy: new insights into how mammalian eggs prevent fertilisation by multiple spermAllison J Gardner
Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, MD 21205, USA
Reprod Fertil Dev 18:53-61. 2006....
- Characterization of the binding of recombinant mouse sperm fertilin alpha subunit to mouse eggs: evidence for function as a cell adhesion molecule in sperm-egg bindingJ P Evans
Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
Dev Biol 187:94-106. 1997..These data are the first evidence to suggest that fertilin alpha can function as a cell adhesion molecule during fertilization, mediating the binding of sperm and egg plasma membranes...
- Calmodulin-dependent protein kinase II triggers mouse egg activation and embryo development in the absence of Ca2+ oscillationsJason G Knott
Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia, PA 19104, USA
Dev Biol 296:388-95. 2006..These results strongly suggest that CaMKII is a major integrator of the Ca(2+) changes that occur following fertilization...
- Sperm incorporation in Xenopus laevis: characterisation of morphological events and the role of microfilamentsJ A Boyle
Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins 60614, USA
Zygote 9:167-81. 2001..Preincubation of eggs in jasplakinolide before latrunculin mitigated both these effects to some degree. However, eggs incubated in latrunculin either prior to or after insemination never progressed through first cleavage...
- Role for sperm in spatial patterning of the early mouse embryoK Piotrowska
Wellcome/CRC Institute and Department of Genetics, University of Cambridge, UK
Nature 409:517-21. 2001..We present a model for axial development that accommodates these findings with the regulative nature of mouse embryos...
- Subcellular localization of beta1,4-galactosyltransferase on bull sperm and its function during sperm-egg interactionsM W Tengowski
Wisconsin Regional Primate Research Center and Department of Zoology, University of Wisconsin, Madison, Wisconsin, USA
Mol Reprod Dev 58:236-44. 2001..The function of GalT in both the murine and bovine systems suggests that it may serve as a generalized gamete receptor in mammals...
- Can we use in vitro fertilization tests to predict semen fertility?B Larsson
Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Centre of Reproduction in Uppsala CRU, Swedish University of Agricultural Sciences SLU, Uppsala, Sweden
Anim Reprod Sci 60:327-36. 2000..Used either as single tests or in combination with other tests, this type of assay can provide valuable information about a semen donor, an insemination dose or a method of semen preservation...
- Conventional PKCs regulate the temporal pattern of Ca2+ oscillations at fertilization in mouse eggsGuillaume Halet
Department of Physiology, University College London, Gower Street, London WC1E 6BT, England, UK
J Cell Biol 164:1033-44. 2004..Finally, we present evidence that one physiological function of activated cPKCs in fertilized eggs is to sustain long-lasting Ca2+ oscillations, presumably via the regulation of store-operated Ca2+ entry...
- Sperm disintegrins, egg integrins, and other cell adhesion molecules of mammalian gamete plasma membrane interactionsJ P Evans
Division of Reproductive Biology, Department of Biochemistry, School of Hygiene and Public Health, Johns Hopkins University, Baltimore, MD, USA
Front Biosci 4:D114-31. 1999....
- Is the oocyte a non-professional phagocyte?R Bronson
Department of Obstetrics and Gynecology, Health Sciences Center, State University of New York, New York 11794 8091, USA
Hum Reprod Update 4:763-75. 1998..It asks whether the oocyte might not utilize similar mechanisms during its incorporation of the spermatozoon...
- Sperm-egg fusion: events at the plasma membraneKathryn K Stein
Section of Molecular and Cellular Biology, School of Medicine, University of California Davis, Davis, CA 95616, USA
J Cell Sci 117:6269-74. 2004..These discoveries provide a strong starting point for the identification of additional proteins that have roles in sperm-egg fusion...
- Multiple proteins present in purified porcine sperm apical plasma membranes interact with the zona pellucida of the oocyteRenske A van Gestel
Department of Biochemistry and Cell Biology, Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands
Mol Hum Reprod 13:445-54. 2007..Although we were not able to identify all of the proteins involved, this is a first step in understanding the event of primary sperm-zona interactions and the relevance of this for fertilization is discussed...
- Transfer of oocyte membrane fragments to fertilizing spermatozoaVirginie Barraud-Lange
Universite Paris 13, UFR SMBH, Laboratoire de Biologie de la Reproduction, and AP HP Hôpital Jean Verdier, Bondy, France
FASEB J 21:3446-9. 2007..Acquisition of CD9 by the sperm may be crucial for the membrane reorganization in sperm required for fusion with the oocyte, a process that is similar to the role CD9 plays in oocyte membrane reorganization...
- Participation of the nonreducing terminal beta-galactosyl residues of the neutral N-linked carbohydrate chains of porcine zona pellucida glycoproteins in sperm-egg bindingN Yonezawa
Graduate School of Science and Technology, Chiba University, 1 33 Yayoi cho, Inage Ku, Chiba shi, Chiba 163 8522, Japan
Mol Reprod Dev 70:222-7. 2005..A correlation between the sperm-binding activity and in vitro fertilization rate is also presented...
- Characterization of the binding of recombinant mouse sperm fertilin beta subunit to mouse eggs: evidence for adhesive activity via an egg beta1 integrin-mediated interactionJ P Evans
Center for Research on Reproduction and Women s Health, University of Pennsylvania, Philadelphia 19104, USA
Dev Biol 187:79-93. 1997..These results suggest that a beta1-containing integrin participates in the binding of recombinant fertilin beta-EC to mouse eggs...
- Reduced oocyte activation and first cleavage rate after ICSI with spermatozoa from a sterile mouse chromosome mutantE B Baart
Laboratory of Genetics, Wageningen Institute of Animal Sciences, Wageningen University, 6701BH Wageningen, The Netherlands
Hum Reprod 19:1140-7. 2004..Male mice, heterozygous for two semi-identical reciprocal translocations T(1;13)70H and T(1;13)1Wa are usually sterile. We have investigated this oligoasthenoteratozoospermic mouse model using ICSI...
- Fertilization and inositol 1,4,5-trisphosphate (IP3)-induced calcium release in type-1 inositol 1,4,5-trisphosphate receptor down-regulated bovine eggsChristopher Malcuit
Department of Veterinary and Animal Sciences, University of Massachusetts, Amherst, 01003, USA
Biol Reprod 73:2-13. 2005..Together, these results demonstrate the essential role of IP(3)R-1-mediated Ca(2+) release during fertilization in bovine eggs, and identify cell cycle regulatory mechanisms of [Ca(2+)](i) oscillations at the level of IP(3)R-1...
- Expression and structure-function analysis of de, a sperm cysteine-rich secretory protein that mediates gamete fusionDiego A Ellerman
Instituto de Biologia y Medicina Experimental, 1428 Buenos Aires, Argentina
Biol Reprod 67:1225-31. 2002..To our knowledge, this is the first study reporting the relevance of structural components for the function of a CRISP member...
- Mouse sperm-egg plasma membrane interactions: analysis of roles of egg integrins and the mouse sperm homologue of PH-30 (fertilin) betaJ P Evans
Department of Obstetrics and Gynecology, School of Medicine, University of Pennsylvania, Philadelphia 19104, USA
J Cell Sci 108:3267-78. 1995....
- Role of MAP kinase and myosin light chain kinase in chromosome-induced development of mouse egg polarityManqi Deng
Department of Biology, University of Pennsylvania, 415 South University Avenue, Philadelphia, PA 19104 6018, USA
Dev Biol 278:358-66. 2005..These results provide new insights into how cortical reorganization occurs independently of extracellular signals to generate egg polarity...
- Gonadotropin exposure, salt storage and storage duration affect penetration of domestic cat oocytes by homologous spermatozoaJ R Herrick
Center for Conservation and Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, 3400 Vine Street, OH 45220 1399, USA
Theriogenology 59:1503-13. 2003..05) in fresh IVM2 oocytes than in oocytes stored for 2-3 weeks or 2-3 months. These results suggest that oocyte penetration is improved by IVM, but is impaired by exposure to salt-storage solution and prolonged storage duration...
- The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggsNaokazu Inoue
Genome Information Research Center, Osaka University, Yamadaoka 3 1, Suita, Osaka 565 0871, Japan
Nature 434:234-8. 2005..Human sperm also contain Izumo and addition of the antibody against human Izumo left the sperm unable to fuse with zona-free hamster eggs...
- Cytochalasin-D retards sperm incorporation deep into the egg cytoplasm but not membrane fusion with the egg plasma membraneManuel Sánchez-Gutiérrez
Departamento de Biologia Celular, Centro de Investigacón y de Estudios Avanzados del Instituto Politécnico Nacional, Apdo Postal 14 740, Mexico, DF
Mol Reprod Dev 63:518-28. 2002..These results show that actin polymerization is necessary for spermatozoa incorporation deep into the egg cytoplasm, but not for plasma membrane fusion nor egg activation early steps...
- Signalling mechanisms of mammalian oocyte activationCarmen J Williams
Center for Research on Reproduction and Women s Health and Department of Obstetrics and Gynecology, University of Pennsylvania, 19104 6142, USA
Hum Reprod Update 8:313-21. 2002..Finally, the mechanisms of physiological oocyte activation are compared with events of oocyte activation that occur after ICSI...
- Specific localization in the equatorial region of gp20, a 20 kDa sialylglycoprotein of the capacitated human spermatozoon acquired during epididymal transit which is necessary to penetrate zona-free hamster eggsR Focarelli
Department of Evolutionary Biology, University of Siena, Italy
Mol Hum Reprod 4:119-25. 1998..Anti-gp20 exerted a blocking effect in a test for sperm penetration of zona-free hamster eggs, thus suggesting that gp20 is involved in the early stages of fertilization...
- A short sperm-oocyte incubation time ZBA in the dogUlrika Hermansson
Department of Clinical Sciences, Division of Comparative Reproduction, Obstetrics and Udder Health, Swedish University of Agricultural Sciences, Box 7054, SE 750 07 Uppsala, Sweden
Theriogenology 66:717-25. 2006..Evaluating chilled and frozen-thawed dog spermatozoa using a zona pellucida binding assay...
- Remodelling the paternal chromatin at fertilization in mammalsDavid W McLay
Departments of Obstetrics and Gynecology, Biology and Medicine, McGill University, Room F350, Royal Victoria Hospital, 687 Pine Ave W, Montreal H3A 1A1, Canada
Reproduction 125:625-33. 2003..Identifying the mechanisms that underlie sperm chromatin remodelling at fertilization may be relevant for understanding reprogramming of somatic cell nuclei after transfer into oocytes...
- Alpha 6 beta 1 Integrin is a potential clinical marker for evaluating sperm quality in menVenkata Rami K Reddy
Immunology Laboratory, National Institute for Research in Reproductive Health, Indian Council of Medical Research, Parel, Mumbai, India
Fertil Steril 79:1590-6. 2003..To determine the clinical significance of alpha6beta1 integrin in human spermatozoa...
- The mechanism of sperm-oocyte fusion in mammalsKeisuke Kaji
Institute for Stem Cell Research, The University of Edinburgh, Roger Land Building, The King s Building, West Mains Road, Edinburgh, EH9 3JQ, UK
Reproduction 127:423-9. 2004..In this review we highlight the candidate molecules involved in the sperm-oocyte interaction suggested from the recent progress in this research field...
- When a sperm meets an egg: block to polyspermyAlina Tsaadon
Department of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv 69978, Israel
Mol Cell Endocrinol 252:107-14. 2006..The more we know of the intricate process of CGE and of the proteins participating in it, the more the assisted reproductive procedures might benefit from that knowledge...
- Zona pellucida binding ability and responsiveness to ionophore challenge of cryopreserved dog spermatozoa after different periods of capacitation in vitroA I Peña
Unit of Reproduction and Obstetrics, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Santiago de Compostela, 27002 Lugo, Spain
Anim Reprod Sci 84:193-210. 2004....
- The C. elegans spe-9 gene encodes a sperm transmembrane protein that contains EGF-like repeats and is required for fertilizationA Singson
Department of Biology, Emory University, Atlanta, Georgia 30322, USA
Cell 93:71-9. 1998..These results suggest that SPE-9 functions in the specialized cell-cell interactions required for fertilization...
- Increasing uterine receptivity by decreasing estradiol levels during the preimplantation period in high responders with the use of a follicle-stimulating hormone step-down regimenC Simon
Instituto Valenciano de Infertilidad and Department of Pediatrics, Obstetrics and Gynecology, Valencia University, Spain
Fertil Steril 70:234-9. 1998..To analyze the effect on uterine receptivity of a decrease in E2 levels during the preimplantation period with the use of a step-down regimen in high responders undergoing IVF...
- Polypeptide backbone derived from carboxyl terminal of mouse ZP3 inhibits sperm-zona bindingDing Li
Department of Histology and Embryology, Shanghai Jiaotong University School of Medicine, Shanghai, China
Mol Reprod Dev 74:1327-36. 2007..Our results suggest that the carboxyl terminal of mZP3 polypeptide backbone interacts with sperm and such interaction plays a significant role in sperm-zona binding, ultimately successful fertilization...
- Distribution of alpha-D-mannose residues on zona pellucida and their role(s) in fertilization in pigsXueXiong Song
Faculty of Animal Science and Technology, Laiyang Agricultural College, Qingdao 266109, China
Sci China C Life Sci 50:170-7. 2007..These results suggest that alpha-D-mannose residues presenting on pig ZP might be an important component of sperm receptor and might induce sperm acrosome reaction and thus facilitate the sperm penetration into the ZP...
- New insights into the molecular mechanisms of sperm-egg interactionB Nixon
Reproductive Science Group, ARC Centre of Excellence in Biotechnology and Development, School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW 2308, Australia
Cell Mol Life Sci 64:1805-23. 2007..Herein, we describe two models indicating the plethora of highly orchestrated molecular interactions underlying successful sperm zona binding and sperm oocyte fusion...
- Comparison of the frequency of defective sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction between subfertile men with normal and abnormal semenDe Yi Liu
Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women s Hospital, 132, Grattan Street, Carlton, Victoria 3053, Australia
Hum Reprod 22:1878-84. 2007..e. male partners of infertile couples) with normal and abnormal semen analyses...
- Reassessing the role of progesterone in fertilization--compartmentalized calcium signalling in human spermatozoa?Claire V Harper
School of Biosciences, University of Birmingham, UK
Hum Reprod 20:2675-80. 2005....
- D-Mannose-binding sites are putative sperm determinants of human oocyte recognition and fertilizationGermán Rosano
Laboratorio de Estudios Reproductivos, Cátedra de Bioquímica Clínica, Facultad de Ciencias Bioquimicas y Farmaceuticas, Universidad Nacional de Rosario, San Lorenzo 939 9A 2000, Rosario, Argentina
Reprod Biomed Online 15:182-90. 2007..The data show that D-mannose is involved in the sperm-zona pellucida interaction, and that the expression of MBS on the sperm surface occurs during the acquisition of in-vitro sperm fertilizing ability...
- Premature capacitation of frozen-thawed spermatozoa from subfertile Japanese black cattleKen Kuroda
Graduate School of Science and Technology, Kobe University, Kobe, Japan
J Reprod Dev 53:1079-86. 2007..It is therefore possible that premature capacitation of sperm used for AI is a causal factor of subfertility of male Japanese Black cattle and a potentially good marker for identification of subfertile bulls for removal from AI programs...
- Cytogenetic analysis of sperm nucleous components of iranian normal and sub-fertile individuals using zona free hamster oocytesH Mozdarani
Department of Infertility Genetics, Royan Institute, Tehran, Iran
J Assist Reprod Genet 21:409-14. 2004..PCC rate was studied for evaluating the role of sperm chromatin abnormalities in the process of nuclear decondensation...
- CaMKII can participate in but is not sufficient for the establishment of the membrane block to polyspermy in mouse eggsAllison J Gardner
Department of Biochemistry and Molecular Biology, Division of Reproductive Biology, Johns Hopkins University, Bloomberg School of Public Health, Baltimore, Maryland 21205, USA
J Cell Physiol 212:275-80. 2007....
- Production of normal offspring from partially zona-incised vitrified mouse oocytes fertilized with cryopreserved spermatozoa using an optimized protocolYan Ping Wang
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing, China
Cryo Letters 29:111-9. 2008....
- Sperm membrane physiology and relevance for fertilizationB M Gadella
Utrecht University, Faculty of Veterinary Medicine, Departments of Biochemistry and Cell Biology and of Farm Animal Health, Utrecht, The Netherlands
Anim Reprod Sci 107:229-36. 2008..This paper emphasizes biochemical models for membrane surface modelling and their potential involvement for remodelling the sperm surface in the above described processes...
- Hyperactivation of capacitated human sperm correlates with the zona pellucida-induced acrosome reaction of zona pellucida-bound spermD Y Liu
Department of Obstetrics and Gynaecology, University of Melbourne, Royal Women s Hospital, 132 Grattan Street, Carlton 3053, Australia
Hum Reprod 22:2632-8. 2007..The aim of this study was to determine the relationship between human sperm hyperactivation (HA), sperm-zona pellucida (ZP) binding and the ZP-induced acrosome reaction (AR) of ZP-bound sperm in vitro...
- Egg water from the amphibian Bufo arenarum induces capacitation-like changes in homologous spermatozoaDario Krapf
División Biología del Desarrollo, IBR CONICET UNR and Area Biología, FCByF, UNR, Argentina
Dev Biol 306:516-24. 2007..These data indicate that sperm should undergo a series of molecular changes to gain fertilizing capacity; these changes are reminiscent of mammalian sperm capacitation and take place before the acrosome reaction...
- Evaluation of sperm functional attributes in relation to in vitro sperm-zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (Bubalus bubalis) semen qualityS Selvaraju
Reproductive Physiology Laboratory, Animal Physiology Division, National Institute of Animal Nutrition and Physiology, Adugodi, Bangalore 560 030, India
Anim Reprod Sci 106:311-21. 2008..70) and plasmalemma integrity measured by HOS-G test (r=0.68). The present study indicates that these attributes could represent important determinants of buffalo sperm quality influencing cleavage rate...
- Exposure to trichloroethylene and its metabolites causes impairment of sperm fertilizing ability in miceHongbin Xu
Ottawa Health Research Institute, Hormones, Growth, Development, and Department of Biochemistry Microbiology Immunology, University of Ottawa, Ottawa, Ontario, Canada K1Y 4E9
Toxicol Sci 82:590-7. 2004..1-10 microg/mL), although the decreases were not as pronounced as those for CH. These results showed that TCE exposure leads to impairment of sperm fertilizing ability, which may be attributed to TCE metabolites, CH, and TCOH...
- In vitro penetration of fresh and vitrified swine oocytes by homologous spermatozoa using different incubation systemsMilton C Macedo
Faculdade de Veterinaria, Centro de Biotecnologia, Universidade Federal de Pelotas, 96010 900 Pelotas RS, Brazil
Anim Reprod Sci 92:334-48. 2006..The IVP test was the only method of semen evaluation that identified the reduction in semen quality up to 72 h of storage...
- RANTES and human sperm fertilizing ability: effect on acrosome reaction and sperm/oocyte fusionA Barbonetti
Andrologic Unit, Department of Internal Medicine, University of L Aquila, Blocco 11, Coppito 67100, Coppito, L Aquila, Italy
Mol Hum Reprod 14:387-91. 2008....
- Gamete membrane adhesion and fusion during fertilizationWilliam J Snell; Fiscal Year: 2013..We will identify proteins that interact with HAP2, we will study the functional domains of HAP2, and we will identify new proteins that function during membrane fusion. ..
- The Role of Acid-active Hyaluronidases in Sperm FunctionPATRICIA ANASTASIA DELEON; Fiscal Year: 2010..HYAL2 and 3, acidic hyases, are also expected to play a critical role in IVF and the recombinant proteins can now be used to advance assisted reproductive technology via their addition to the sperm surface, to treat sperm pathology. ..
- Gamete Interactions in Caenorhabditis elegansAndrew W Singson; Fiscal Year: 2012..This work will complement studies of fertilization in other species and could eventually lead to a better understanding of the causes of infertility, the diversity of reproductive strategies and the design of new contraceptives. ..
- ACROSOMAL ENZYMES AND THEIR ROLE IN FERTILIZATIONPRAKASH SRIVASTAVA; Fiscal Year: 1993..The role of acrosomal enzymes in sperm-egg binding, and the ovum penetration will be studied. Enzymes will also be tested on isolated zona...
- EPIDEMIOLOGIC AND BIOLOGIC PREDICTORS OF IVF SUCCESSDaniel Cramer; Fiscal Year: 2002....
- STUDIES ON SPERM FERTILIZING ABILITYBarry Bavister; Fiscal Year: 1993..The results of these experiments will provide deeper insights into the functional roles of these naturally-occurring factors, and into their mechanisms of action in regulating events immediately preceding egg penetration...
- Fertilization and Activation of Development 2007 Gordon Research ConferenceGEORGE GERTON; Fiscal Year: 2007..The conference will promote collaborations among US [unreadable] and international scientists with widely disparate experiences/seniority, methodologies, [unreadable] and model systems. [unreadable] [unreadable] [unreadable] [unreadable]..
- RECEPTOR OCCUPANCY DURING INITIAL GAMETE INTERACTIONSCATHERINE THALER; Fiscal Year: 2000..Understanding the mechanisms regulating the acrosome reaction is essential for our understanding of fertilization and for developing effective alternatives to control fertilization. ..
- Gordon Conference/Fertilization/Activation of DevelopmRICHARD CARDULLO; Fiscal Year: 2003..These latter talks will particularly feature junior scientists, including postdoctoral fellows and students. In addition, we will have a keynote speaker for the Tuesday evening session. ..
- FUNCTIONS OF PROTEIN DOMAINS IN GAMETE INTERACTIONSDaniel Hardy; Fiscal Year: 2002..By adding to general knowledge of cell-cell interactions, they may also lead to a better understanding of other medical conditions such as development problems, cancer, blood disorders, and neurological diseases. ..
- FRONTIERS IN REPRODUCTION: MOLECULAR & CELLULAR CONCEPTSGerald Schatten; Fiscal Year: 2001..abstract_text> ..
- SPAM 1 ALLELES, SPERM PHENOTYPE AND FUNCTIONPatricia DeLeon; Fiscal Year: 2004....
- Recombinant Antibodies as Intra-Vaginal SpermicidesMichael Coppola; Fiscal Year: 2002..Finally, the stability and safety of the optimized formulation will be assessed. PROPOSED COMMERCIAL APPLICATION: Topical intra-vaginal contraceptive. ..
- Preimplantation Embryo Blastomere FragmentationKeith Latham; Fiscal Year: 2006..Last, because studies such as those proposed cannot be conducted in the human, the proposed studies will provide unprecedented data regarding the relationship between fragmentation and viability. ..
- CELL ADHESION MOLECULES OF MAMMALIAN FERTILIZATIONJanice Evans; Fiscal Year: 2008..This research also will also address fundamental questions in cell adhesion biology by examining this interesting, biologically relevant cell-cell interaction event. ..
- MECHANISMS OF MAMMALIAN FERTILIZATIONPAUL WASSARMAN; Fiscal Year: 2007..Furthermore, the research could have important consequences for development of novel reversible contraceptive methods that prevent ZP formation during oogenesis in humans. ..
- CENTER FOR DEVELOPMENT OF ANTI-SPERM CONTRACEPTIVESPaul Primakoff; Fiscal Year: 2006..Thus, we have proposed an expansion of our target list in spermatogenesis, increasing our scope and chances of developing new products for birth control. ..
- GLYCOCONJUGATE-INDUCED TOLERANCE IN NATURAL KILLER CELLSGary Clark; Fiscal Year: 2002..The primary goal of this study is to carry out experiments complementing preliminary studies in support of this glycoconjugate-based model. ..
- Potential Role of Glycosylation in AIDS PathogenesisGary Clark; Fiscal Year: 2005..These preliminary studies will be crucial for determining if HIV-1 is employing its carbohydrate sequences as functional groups to mediate immune suppression. ..
- Regulation of Meiotic Arrest in Oocytes by G-ProteinsLISA MEHLMANN; Fiscal Year: 2004..Understanding how the oocyte cell cycle is regulated within the follicle may find applications to practical problems including assisted reproductive techniques, contraception, and infertility. ..
- Mammalian Gamete Surface Organization and FunctionDiana Myles; Fiscal Year: 2008..Thus, these studies are designed to combine molecular and cellular levels to bring new insight into the process of gamete membrane fusion. ..
- PROTOCOLS FOR MATURING AND FREEZING MONKEY OOCYTESCatherine VandeVoort; Fiscal Year: 2006..abstract_text> ..
- Effects of Fetal Bisphenol A Exposure on Oogenesis in PrimatesCATHERINE A contact VANDEVOORT; Fiscal Year: 2010..Because the dose used will be similar to current human blood levels of BPA, the results should have direct relevance to human health. ..
- New Approaches to Rat Sperm CryopreservationJohn Bischof; Fiscal Year: 2006..Specific Aim 3: Design and test strategies for protecting rat sperm from damage incurred by cryopreservation. ..
- Binge Ethanol and Oocyte QualityCatherine A Vandevoort; Fiscal Year: 2010..The outcome of this project will have direct relevance to the effects of binge drinking in women. ..
- KLHL 10-mediated uniquitination during spermiogenesisWei Yan; Fiscal Year: 2010..On the other hand, KLHL10 and its substrate proteins may be good targets for future non-hormonal male contraceptives. ..
- Characterization of a Testis Kinase Family (Tssk)Pablo Visconti; Fiscal Year: 2007..The predicted importance of Tssks in spermiogenesis and/or sperm function suggests that this research can ultimately help to design novel human contraceptives. [unreadable] [unreadable] [unreadable]..
- Vanadocenes as a New Class of Spermicidal DrugsOSMOND D CRUZ; Fiscal Year: 2007..unreadable] [unreadable] [unreadable]..
- Asymmetric cell division in the C elegans embryoRobert Goldstein; Fiscal Year: 2007..We expect that we will learn important new information about how cells divide asymmetrically that will suggest mechanisms and guide studies in mammals, including humans. ..
- EMBRYO TECHNOLOGIES FOR PROPAGATION OF RHESUS MONKEYSBarry Bavister; Fiscal Year: 2006..abstract_text> ..
- PROFESSORS OF THE FUTURE: POST DOC CAREER DEVELOPMENTJERRY HEDRICK; Fiscal Year: 2006..abstract_text> ..
- Molecular genetics of egg activation and pronuclear function in DrosophilaMARIANA FEDERICA WOLFNER; Fiscal Year: 2010..Thus, we exploit the powerful genetics of Drosophila to elucidate efficiently the molecules and gene pathways needed to start development in humans. ..
- CELL-SUBSTRATE INTERACTION IN CRANIOFACIAL MORPHOGENESISBARRY SHUR; Fiscal Year: 2009..It is anticipated, that these results will identify glycosyltransferases that play key roles in early embryonic and craniofacial development, and which can then be analyzed in appropriate mammalian model systems. ..
- MOLECULAR MECHANISMS OF VIRAL MEMBRANE FUSION PROTEINSJudith White; Fiscal Year: 2009..g. influenza, West Nile, and Ebola viruses). The proposed work will aid these important efforts. ..
- Role of galectin-3 in genital tract immunosuppressionAlan Diekman; Fiscal Year: 2008..unreadable] [unreadable]..
- Gamete Interactions in Caenorhabditis elegansAndrew Singson; Fiscal Year: 2007..This work will complement fertility studies in other organisms as well as provide insights into the mechanisms of cell-cell interactions and the diversity of reproductive strategies. [unreadable] [unreadable] [unreadable]..
- EVOLUTIONARY GENETICS OF MALE REPRODUCTIVE TRAITS IN MUSMATTHEW DEAN; Fiscal Year: 2006..These studies will help uncover the genetic basis of male reproductive fitness in a natural population of house mice. [unreadable] [unreadable]..
- Role of GPI-Anchored CD52 Glycoprotein in Sperm FunctionAlan Diekman; Fiscal Year: 2003..These studies will provide insights into the function of sperm CD52 and into the signal transduction mechanisms involved in the AR. ..
- MAMMALIAN SPERM SURFACE MANNOSIDASEDAULAT TULSIANI; Fiscal Year: 2003....
- Proteomics of Egg Cortical GranulesLEANN LINDSAY; Fiscal Year: 2003..This approach will identify potential molecules important in egg extracellular matrix modifications, which will be the subject of future investigations. ..
- PHI 346: A Novel Dual-Function MicrobicideOSMOND D CRUZ; Fiscal Year: 2003..Following successful completion of the Phase I in vivo efficacy studies, gel formulation of PHI-346 will be further explored as a vaginal dual-function microbicide in Phase II. ..
- FLP-102: Rationally Engineered Antiviral ProteinOSMOND D CRUZ; Fiscal Year: 2003..The preclinical data on FLP-102 will be essential to further explore the utility of this novel recombinant PAP mutant for Phase II. ..
- ACQUISITION OF A MASS SPECTROMETRY RESOURCELeslie Goodwin; Fiscal Year: 2003..In order for science at the Institute to continue at a competitive pace and for our investigators to expand their research capabilities and horizons, we need to establish a shared mass spectrometer resource. ..
- IONIC REGULATION IN THE ACTIVATION OF DEVELOPMENTJERRY HEDRICK; Fiscal Year: 2002..abstract_text> ..
- MAMMALIAN SPERM SURFACE ORGANIZATION AND FUNCTIONDiana Myles; Fiscal Year: 2002..Furthermore, the identified, functional gamete proteins are potential targets for novel contraceptive methods based on pharmacological and immunological approaches. ..
- STRUCTURE/FUNCTION OF ZONA PELLUCIDA GLYCOPROTEINSDAULAT TULSIANI; Fiscal Year: 2001....
- METVAN: A Novel Anticancer AgentOSMOND D CRUZ; Fiscal Year: 2003..The knowledge gained from these studies described under Specific Aims 1-2 is expected to facilitate the design of innovative treatment regimens employing METVAN for metastatic breast cancer and brain tumor patients. ..
- ADAMS--ADHESION, FUSION AND SIGNALING ACTIVITIESJudith White; Fiscal Year: 2004..For example, the dual functions of ADAMs as both adhesion molecules and as proteases suggests that they may be key players in the de-adhesive, migratory, and re-adhesive activities involved in tumor metastasis. ..
- GLYCOSYLTRANSFERASE FUNCTION DURING FERTILIZATIONBARRY SHUR; Fiscal Year: 2006..Finally, in the third Aim, we will continue our analysis of GalT-mediated signal transduction leading to the acrosome reaction. ..
- Stampidine: A Novel Broad-Spectrum Anti-HIV MicrobicideOSMOND D CRUZ; Fiscal Year: 2005..Specific Aims 1 and 2 may provide the foundation for the clinical development of STAMP in Phase II studies as a safe, effective broad-spectrum anti-HIV microbicide without conception-inhibiting functions. ..
- Phase II SBIR: WHI-07: A Novel Dual-Function MicrobicideOSMOND D CRUZ; Fiscal Year: 2005....
- Gamete Interactions in Caenorhabditis elegansAndrew Singson; Fiscal Year: 2005..This work will provide new insight into cell-cell interactions, conception and complement studies of fertilization in other organisms. ..
- Fusion Mechanism of the Ebola Virus GlycoproteinJudith White; Fiscal Year: 2004..The stage would then be set to screen for inhibitors of Ebola virus fusion and to test their efficacy in animal models. ..
- Supporting Human ART Through Basic ScienceBarry Bavister; Fiscal Year: 2004..This Symposium will attract members of the human ART community and scientists working in animal ART, and will provide a basis for continued and expanded interactions between these two groups. ..
- PHI-443: Novel Non-Spermicidal Anti-HIV MicrobicideOSMOND D CRUZ; Fiscal Year: 2003..Specific Aims 1 and 2 may provide the foundation for the clinical development of PHI-443 in Phase II studies as a safe, effective broad-spectrum anti-HIV microbicide without conception-inhibiting functions. ..
- NOVEL CONTRACEPTIVES WITH ANTI-HIV ACTIVITYOSMOND D CRUZ; Fiscal Year: 2001..The preclinical data on in vivo efficacies of WHI-05 and WHI-07 will be essential to further explore the utility of these novel drugs for clinical studies in human patients. ..