Summary: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.

Top Publications

  1. doi Molecular mechanism and physiological functions of clathrin-mediated endocytosis
    Harvey T McMahon
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nat Rev Mol Cell Biol 12:517-33. 2011
  2. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
  3. pmc Endosome maturation
    Jatta Huotari
    Institute of Biochemistry, ETH Zurich, Switzerland
    EMBO J 30:3481-500. 2011
  4. pmc Trans-endocytosis of CD80 and CD86: a molecular basis for the cell-extrinsic function of CTLA-4
    Omar S Qureshi
    Medical Research Council MRC Centre for Immune Regulation, School of Immunity and Infection, Institute of Biomedical Research, University of Birmingham Medical School, Birmingham B15 2TT, UK
    Science 332:600-3. 2011
  5. pmc Increased expression of alpha-synuclein reduces neurotransmitter release by inhibiting synaptic vesicle reclustering after endocytosis
    Venu M Nemani
    Departments of Neurology and Physiology, Graduate Program in Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA
    Neuron 65:66-79. 2010
  6. pmc Endocytosis and signalling: intertwining molecular networks
    Alexander Sorkin
    Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, 80010, USA
    Nat Rev Mol Cell Biol 10:609-22. 2009
  7. pmc A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis
    Marcus J Taylor
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    PLoS Biol 9:e1000604. 2011
  8. doi Population context determines cell-to-cell variability in endocytosis and virus infection
    Berend Snijder
    Institute of Molecular Systems Biology, ETH Zurich Swiss Federal Institute of Technology, Wolfgang Pauli Strasse 16, CH 8093 Zurich, Switzerland
    Nature 461:520-3. 2009
  9. doi Virus entry by endocytosis
    Jason Mercer
    ETH Zurich, Institute of Biochemistry, CH 8093 Zurich, Switzerland
    Annu Rev Biochem 79:803-33. 2010
  10. doi Integrins: masters and slaves of endocytic transport
    Patrick T Caswell
    Beatson Institute for Cancer Research, Cancer Research UK, Glasgow, G61 3BX, Scotland
    Nat Rev Mol Cell Biol 10:843-53. 2009

Detail Information

Publications426 found, 100 shown here

  1. doi Molecular mechanism and physiological functions of clathrin-mediated endocytosis
    Harvey T McMahon
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    Nat Rev Mol Cell Biol 12:517-33. 2011
    Clathrin-mediated endocytosis is the endocytic portal into cells through which cargo is packaged into vesicles with the aid of a clathrin coat...
  2. ncbi Phosphoinositides in cell regulation and membrane dynamics
    Gilbert Di Paolo
    Department of Pathology and Cell Biology, The Taub Institute for Research on Alzheimer s Disease and the Aging Brain, Columbia University Medical Center, New York, New York 10032, USA
    Nature 443:651-7. 2006
    ..Their functions, besides classical signal transduction at the cell surface, include regulation of membrane traffic, the cytoskeleton, nuclear events and the permeability and transport functions of membranes...
  3. pmc Endosome maturation
    Jatta Huotari
    Institute of Biochemistry, ETH Zurich, Switzerland
    EMBO J 30:3481-500. 2011
    ..The maturation programme entails a dramatic transformation of these dynamic organelles disconnecting them functionally and spatially from early endosomes and preparing them for their unidirectional role as a feeder pathway to lysosomes...
  4. pmc Trans-endocytosis of CD80 and CD86: a molecular basis for the cell-extrinsic function of CTLA-4
    Omar S Qureshi
    Medical Research Council MRC Centre for Immune Regulation, School of Immunity and Infection, Institute of Biomedical Research, University of Birmingham Medical School, Birmingham B15 2TT, UK
    Science 332:600-3. 2011
    ..Here, we show that CTLA-4 can capture its ligands from opposing cells by a process of trans-endocytosis. After removal, these costimulatory ligands are degraded inside CTLA-4-expressing cells, resulting in impaired ..
  5. pmc Increased expression of alpha-synuclein reduces neurotransmitter release by inhibiting synaptic vesicle reclustering after endocytosis
    Venu M Nemani
    Departments of Neurology and Physiology, Graduate Program in Neuroscience, University of California, San Francisco, San Francisco, CA 94158, USA
    Neuron 65:66-79. 2010
    ..density at the active zone, and imaging further reveals a defect in the reclustering of synaptic vesicles after endocytosis. Increased levels of alpha-synuclein thus produce a specific, physiological defect in synaptic vesicle ..
  6. pmc Endocytosis and signalling: intertwining molecular networks
    Alexander Sorkin
    Department of Pharmacology, University of Colorado Denver School of Medicine, Aurora, 80010, USA
    Nat Rev Mol Cell Biol 10:609-22. 2009
    ..Indeed, many recent examples illustrate how endocytosis regulates receptor signalling (including signalling from receptor tyrosine kinases and G protein-coupled ..
  7. pmc A high precision survey of the molecular dynamics of mammalian clathrin-mediated endocytosis
    Marcus J Taylor
    Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom
    PLoS Biol 9:e1000604. 2011
    ..reflection fluorescence microscopy is a powerful tool for decoding the molecular dynamics of clathrin-mediated endocytosis (CME)...
  8. doi Population context determines cell-to-cell variability in endocytosis and virus infection
    Berend Snijder
    Institute of Molecular Systems Biology, ETH Zurich Swiss Federal Institute of Technology, Wolfgang Pauli Strasse 16, CH 8093 Zurich, Switzerland
    Nature 461:520-3. 2009
    ..analyse, for the first time, the heterogeneous patterns of related cellular activities, namely virus infection, endocytosis and membrane lipid composition in adherent human cells...
  9. doi Virus entry by endocytosis
    Jason Mercer
    ETH Zurich, Institute of Biochemistry, CH 8093 Zurich, Switzerland
    Annu Rev Biochem 79:803-33. 2010
    ..The internalization may involve clathrin-mediated endocytosis (CME), macropinocytosis, caveolar/lipid raft-mediated endocytosis, or a variety of other still poorly ..
  10. doi Integrins: masters and slaves of endocytic transport
    Patrick T Caswell
    Beatson Institute for Cancer Research, Cancer Research UK, Glasgow, G61 3BX, Scotland
    Nat Rev Mol Cell Biol 10:843-53. 2009
    ..Furthermore, integrins can exert control over the trafficking of other receptors in a way that drives cancer cell invasion and tumour angiogenesis...
  11. ncbi The small GTPase rab5 functions as a regulatory factor in the early endocytic pathway
    C Bucci
    European Molecular Biology Laboratory, Heidelberg, Germany
    Cell 70:715-28. 1992
    ..In cells expressing the rab5ile 133 protein, the rate of endocytosis was decreased by 50% compared with normal, while the rate of recycling was not significantly affected...
  12. pmc Pathways and mechanisms of endocytic recycling
    Barth D Grant
    Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854, USA
    Nat Rev Mol Cell Biol 10:597-608. 2009
    ..recycling has come from studies on the transferrin receptor, a protein internalized through clathrin-dependent endocytosis, increased interest in clathrin-independent endocytosis has led to the discovery of new endocytic recycling ..
  13. doi Tickets to ride: selecting cargo for clathrin-regulated internalization
    Linton M Traub
    Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine, Pennsylvania 15261, USA
    Nat Rev Mol Cell Biol 10:583-96. 2009
    Clathrin-mediated endocytosis oversees the constitutive packaging of selected membrane cargoes into transport vesicles that fuse with early endosomes...
  14. ncbi Lipid rafts and signal transduction
    K Simons
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 110, D 01307 Dresden, Germany
    Nat Rev Mol Cell Biol 1:31-9. 2000
    ..Lipid rafts containing a given set of proteins can change their size and composition in response to intra- or extracellular stimuli. This favours specific protein-protein interactions, resulting in the activation of signalling cascades...
  15. doi The roles of flotillin microdomains--endocytosis and beyond
    Grant P Otto
    Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 0QH, UK
    J Cell Sci 124:3933-40. 2011
    ..Flotillins have been implicated in myriad processes that include endocytosis, signal transduction and regulation of the cortical cytoskeleton, yet the molecular mechanisms that underlie ..
  16. pmc TRAM couples endocytosis of Toll-like receptor 4 to the induction of interferon-beta
    Jonathan C Kagan
    Howard Hughes Medical Institute and Department of Immunobiology, Yale University School of Medicine, New Haven, Connecticut 06520, USA
    Nat Immunol 9:361-8. 2008
    ..Here we show that TLR4 activated these two signaling pathways sequentially in a process organized around endocytosis of the TLR4 complex...
  17. ncbi Protein regulation by monoubiquitin
    L Hicke
    Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University Evanston, Illinois 60208, USA
    Nat Rev Mol Cell Biol 2:195-201. 2001
    ..Instead of sending proteins to their death through the proteasome, monoubiquitylation regulates processes that range from membrane transport to transcriptional regulation...
  18. doi Virus entry by macropinocytosis
    Jason Mercer
    ETH Zurich, Institute of Biochemistry, Schafmattsrasse 18, ETH Hoenggerberg, Zurich 8093, Switzerland
    Nat Cell Biol 11:510-20. 2009
    ..We review the molecular machinery involved in macropinocytosis and describe what is known about its role in virus entry...
  19. pmc Receptor-mediated endocytosis in the Caenorhabditis elegans oocyte
    B Grant
    Columbia University College of Physicians and Surgeons, Department of Biochemistry and Molecular Biophysics, New York, New York 10032, USA
    Mol Biol Cell 10:4311-26. 1999
    ..elegans oocyte is a highly amenable system for forward and reverse genetic analysis of receptor-mediated endocytosis. We describe the use of transgenic strains expressing a vitellogenin::green fluorescent protein (YP170::GFP) ..
  20. pmc CD14 controls the LPS-induced endocytosis of Toll-like receptor 4
    Ivan Zanoni
    Harvard Medical School and Division of Gastroenterology, Children s Hospital Boston, Boston, MA 02115, USA
    Cell 147:868-80. 2011
    ..that the plasma membrane localized Pattern Recognition Receptor (PRR) CD14 is required for the microbe-induced endocytosis of TLR4...
  21. doi Notch signaling: the core pathway and its posttranslational regulation
    Mark E Fortini
    Department of Biochemistry and Molecular Biology, Kimmel Cancer Center, Thomas Jefferson University, Philadelphia, PA 19107, USA
    Dev Cell 16:633-47. 2009
    ..This review describes the core developmental logic of Notch signaling and how regulatory mechanisms tailor Notch pathway outputs to specific developmental scenarios...
  22. ncbi Endocytosis and intracellular trafficking of ErbBs
    Alexander Sorkin
    Department of Pharmacology, University of Colorado Denver Anschutz Medical Center, Aurora, Colorado 80045 0508, USA
    Exp Cell Res 315:683-96. 2009
    This review article describes the pathways and mechanisms of endocytosis and post-endocytic sorting of the EGF receptor (EGFR/ErbB1) and other members of the ErbB family...
  23. ncbi A receptor-mediated pathway for cholesterol homeostasis
    M S Brown
    Science 232:34-47. 1986
  24. ncbi Dynasore, a cell-permeable inhibitor of dynamin
    Eric Macia
    Department of Cell Biology, Harvard Medical School and the CBR Institute for Biomedical Research, Inc, 200 Longwood Avenue, Boston, Massachusetts 02115, USA
    Dev Cell 10:839-50. 2006
    ..Thus, dynamin acts at two steps during clathrin coat formation; GTP hydrolysis is probably needed at both steps...
  25. doi Dynamin: functional design of a membrane fission catalyst
    Sandra L Schmid
    Department of Cell Biology, The Scripps Research Institute, La Jolla, California 92037, USA
    Annu Rev Cell Dev Biol 27:79-105. 2011
    Dynamin, best studied for its role in clathrin-mediated endocytosis, is the prototypical member of a family of multidomain GTPases involved in fission and remodeling of multiple organelles...
  26. ncbi Uptake pathways and subsequent intracellular trafficking in nonviral gene delivery
    Ikramy A Khalil
    Graduate School of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan
    Pharmacol Rev 58:32-45. 2006
    ..In addition, available knowledge concerning cellular entry and the intracellular trafficking of cationic lipid-DNA complexes (lipoplexes) and cationic polymer-DNA complexes (polyplexes) is summarized...
  27. ncbi A modular design for the clathrin- and actin-mediated endocytosis machinery
    Marko Kaksonen
    Department of Molecular and Cell Biology, University of California, Berkeley, 94720, USA
    Cell 123:305-20. 2005
    b>Endocytosis depends on an extensive network of interacting proteins that execute a series of distinct subprocesses...
  28. ncbi Functions of lipid rafts in biological membranes
    D A Brown
    Department of Biochemistry and Cell Biology, State University of New York at Stony Brook 11794 5215, USA
    Annu Rev Cell Dev Biol 14:111-36. 1998
    ..Rafts may also function in sorting and trafficking through the secretory and endocytic pathways...
  29. doi Derailed endocytosis: an emerging feature of cancer
    Yaron Mosesson
    Department of Biological Regulation, The Weizmann Institute of Science, Rehovot 76100, Israel
    Nat Rev Cancer 8:835-50. 2008
    ..In line with the cooperative nature of endocytic machineries, multiple oncogenic alterations underlie defective endocytosis, such as altered ubiquitylation (Cbl and Nedd4 ubiquitin ligases, for example), altered cytoskeletal ..
  30. pmc Endocytosis of nanomedicines
    Gaurav Sahay
    Department of Pharmaceutical Sciences and Center for Drug Delivery and Nanomedicine, College of Pharmacy, M V Lomonosov Moscow State University, 119899 Moscow, Russia
    J Control Release 145:182-95. 2010
    ..This review, first, classifies various mechanisms of endocytosis available to nanomedicines including phagocytosis and pinocytosis through clathrin-dependent and clathrin-..
  31. pmc Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells
    Amy T Ma
    Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA
    Cell Host Microbe 5:234-43. 2009
  32. pmc A FAM21-containing WASH complex regulates retromer-dependent sorting
    Timothy S Gomez
    Department of Immunology, Division of Oncology Research and Schulze Center for Novel Therapeutics, College of Medicine, Mayo Clinic, Rochester, MN 55905, USA
    Dev Cell 17:699-711. 2009
    The Arp2/3 complex regulates endocytosis, sorting, and trafficking, yet the Arp2/3-stimulating factors orchestrating these distinct events remain ill defined...
  33. pmc Hepatitis C virus entry depends on clathrin-mediated endocytosis
    Emmanuelle Blanchard
    Equipe Hepatite C, CNRS UMR8161, Institut de Biologie de Lille, 1 rue du Professeur Calmette, BP447, 59021 Lille Cedex, France
    J Virol 80:6964-72. 2006
    ..The functional role of clathrin-mediated endocytosis in HCV entry was assessed by small interfering RNA-mediated clathrin heavy chain depletion and with ..
  34. pmc ABP1 mediates auxin inhibition of clathrin-dependent endocytosis in Arabidopsis
    Stephanie Robert
    Department of Plant Systems Biology, VIB, 9052 Gent, Belgium
    Cell 143:111-21. 2010
    ..ABP1 acts as a positive factor in clathrin recruitment to the plasma membrane, thereby promoting endocytosis. Auxin binding to ABP1 interferes with this action and leads to the inhibition of clathrin-mediated endocytosis...
  35. ncbi Elucidating the mechanism of cellular uptake and removal of protein-coated gold nanoparticles of different sizes and shapes
    B Devika Chithrani
    Institute of Biomaterials and Biomedical Engineering, Terrence Donnelly Center for Cellular and Biomolecular Research, University of Toronto, 160 College Street, 4th Floor, Toronto, Ontario M5S 3G9, Canada
    Nano Lett 7:1542-50. 2007
    ..We determined that transferrin-coated Au NP entered the cells via clathrin-mediated endocytosis pathway. The NPs exocytosed out of the cells in a linear relationship to size...
  36. pmc Ebola virus enters host cells by macropinocytosis and clathrin-mediated endocytosis
    Paulina Aleksandrowicz
    Institute of Anatomy and Vascular Biology, Westfälische Wilhelms University Muenster, Vesaliusweg, Germany
    J Infect Dis 204:S957-67. 2011
    ..composed of the glycoprotein GP(1,2) and the matrix protein VP40 use macropinocytosis and clathrin-mediated endocytosis to enter cells...
  37. pmc Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis
    Joanna Rejman
    Department of Membrane Cell Biology, University of Groningen, A Deusinglaan 1, 9713 AV Groningen, The Netherlands
    Biochem J 377:159-69. 2004
    ..The clathrin-mediated pathway of endocytosis shows an upper size limit for internalization of approx...
  38. doi Clathrin-independent endocytosis: mechanisms and function
    Kirsten Sandvig
    Department of Biochemistry, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, Oslo, Norway
    Curr Opin Cell Biol 23:413-20. 2011
    It is now about 20 years since we first wrote reviews about clathrin-independent endocytosis. The challenge at the time was to convince the reader about its existence...
  39. pmc Mis-assembly of clathrin lattices on endosomes reveals a regulatory switch for coated pit formation
    L h Wang
    Department of Cell Biology and Neuroscience, University of Texas Southwestern Medical Center, Dallas 75235
    J Cell Biol 123:1107-17. 1993
    ..These effects suggest that CADs reverse an on/off switch that controls AP-2 binding to membranes. We conclude that cells have a mechanism for switching on and off AP-2 binding during the endocytic cycle...
  40. ncbi Auxin inhibits endocytosis and promotes its own efflux from cells
    Tomasz Paciorek
    Zentrum für Molekularbiologie der Pflanzen, Universitat Tubingen, 72076 Tubingen, Germany
    Nature 435:1251-6. 2005
    ..Here we show that a major regulator of plant development, auxin, inhibits endocytosis. This effect is specific to biologically active auxins and requires activity of the Calossin-like protein BIG...
  41. pmc Als3 is a Candida albicans invasin that binds to cadherins and induces endocytosis by host cells
    Quynh T Phan
    Department of Medicine, Los Angeles Biomedical Research Institute, Harbor UCLA Medical Center, Torrance, California, United States of America
    PLoS Biol 5:e64. 2007
    ..albicans hyphae invade endothelial cells and oral epithelial cells in vitro by inducing their own endocytosis. Therefore, we set out to identify the fungal surface protein and host cell receptors that mediate this process...
  42. pmc Dynamin, a membrane-remodelling GTPase
    Shawn M Ferguson
    Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Nat Rev Mol Cell Biol 13:75-88. 2012
    ..In addition, several connections between dynamin and human disease have also emerged, highlighting specific contributions of this GTPase to the physiology of different tissues...
  43. pmc Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
    Mark T Howes
    The Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, The University of Queensland, Brisbane, Queensland 4072, Australia
    J Cell Biol 190:675-91. 2010
    ..Consistent with this, CLIC-mediated endocytosis of key cargo proteins, CD44 and Thy-1, is polarized at the leading edge of migrating fibroblasts, while ..
  44. ncbi Distinct endocytic pathways regulate TGF-beta receptor signalling and turnover
    Gianni M Di Guglielmo
    Programme in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada
    Nat Cell Biol 5:410-21. 2003
    b>Endocytosis of cell surface receptors is an important regulatory event in signal transduction...
  45. ncbi Hakai, a c-Cbl-like protein, ubiquitinates and induces endocytosis of the E-cadherin complex
    Yasuyuki Fujita
    Max Delbruck Center for Molecular Medicine, Robert Rossle Str 10, 13125 Berlin, Germany
    Nat Cell Biol 4:222-31. 2002
    ..kinases induce the tyrosine phosphorylation and ubiquitination of the E-cadherin complex, which induces endocytosis of E-cadherin...
  46. ncbi Virus entry: open sesame
    Mark Marsh
    Cell Biology Unit, MRC Laboratory for Molecular Cell Biology, and Department of Biochemistry and Molecular Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom
    Cell 124:729-40. 2006
    ..These insights are revolutionizing our understanding of endocytosis and the movement of vesicles within cells...
  47. pmc Synaptic vesicle endocytosis
    Yasunori Saheki
    Department of Cell Biology, Howard Hughes Medical Institute and Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut 06510, USA
    Cold Spring Harb Perspect Biol 4:a005645. 2012
    ..These studies have shown that synaptic vesicle endocytosis capitalizes on fundamental and general endocytic mechanisms but also involves neuron-specific adaptations of ..
  48. ncbi APPL proteins link Rab5 to nuclear signal transduction via an endosomal compartment
    Marta Miaczynska
    Max Planck Institute for Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, 01307 Dresden, Germany
    Cell 116:445-56. 2004
    ..We report the identification of a pathway directly linking the small GTPase Rab5, a key regulator of endocytosis, to signal transduction and mitogenesis...
  49. pmc Isoform-specific monoubiquitination, endocytosis, and degradation of alternatively spliced ErbB4 isoforms
    Maria Sundvall
    MediCity Research Laboratory and Department of Medical Biochemistry and Molecular Biology, University of Turku, FIN 20520 Turku, Finland
    Proc Natl Acad Sci U S A 105:4162-7. 2008
    b>Endocytosis and subsequent lysosomal degradation serve as a well characterized mechanism to fine-tune and down-regulate EGFR signaling. However, other members of the EGFR/ErbB receptor family have been reported to be endocytosis-impaired...
  50. ncbi EGF-ERBB signalling: towards the systems level
    Ami Citri
    Department of Biological Regulation, The Weizmann Institute of Science, 1 Hertzl Street, Rehovot 76100, Israel
    Nat Rev Mol Cell Biol 7:505-16. 2006
    ..Because network fragility is an inevitable trade-off of robustness, systems-level understanding is expected to generate therapeutic opportunities to intercept aberrant network activation...
  51. doi The endocytic matrix
    Giorgio Scita
    IFOM, the FIRC Institute for Molecular Oncology Foundation, at the IFOM IEO Campus, Via Adamello 16, 20139, Milan, Italy
    Nature 463:464-73. 2010
    b>Endocytosis has long been thought of as simply a way for cells to internalize nutrients and membrane-associated molecules. But an explosive growth in knowledge has given a new dimension to our understanding of this process...
  52. doi Spatial regulation of Dia and Myosin-II by RhoGEF2 controls initiation of E-cadherin endocytosis during epithelial morphogenesis
    Romain Levayer
    IBDML, UMR6216 CNRS Université de la Méditerranée, Campus de Luminy, Case 907 13288 Marseille Cedex 09, France
    Nat Cell Biol 13:529-40. 2011
    ..we show that E-cadherin polarity is controlled by the polarized regulation of clathrin- and dynamin-mediated endocytosis. Blocking E-cadherin endocytosis resulted in cell intercalation defects...
  53. pmc Rapid and efficient clathrin-mediated endocytosis revealed in genome-edited mammalian cells
    Jeffrey B Doyon
    1 Department of Molecular and Cell Biology, University of California Berkeley, Berkeley, California 94720, USA
    Nat Cell Biol 13:331-7. 2011
    Clathrin-mediated endocytosis (CME) is the best-studied pathway by which cells selectively internalize molecules from the plasma membrane and surrounding environment...
  54. pmc Suppression of EGFR endocytosis by dynamin depletion reveals that EGFR signaling occurs primarily at the plasma membrane
    Leiliane P Sousa
    Departments of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA
    Proc Natl Acad Sci U S A 109:4419-24. 2012
    The role of endocytosis in the control of EGF receptor (EGFR) activation and cell signaling was explored by using mouse fibroblasts in which dynamin was conditionally depleted...
  55. pmc Clathrin-mediated endocytosis in budding yeast
    Jasper Weinberg
    Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720 3202, USA
    Trends Cell Biol 22:1-13. 2012
    Clathrin-mediated endocytosis in the budding yeast Saccharomyces cerevisiae involves the ordered recruitment, activity and disassembly of nearly 60 proteins at distinct sites on the plasma membrane...
  56. doi Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors
    Bon Kyoung Koo
    Hubrecht Institute, KNAW and University Medical Center Utrecht, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    Nature 488:665-9. 2012
    ..We conclude that RNF43 and ZNRF3 reduce Wnt signals by selectively ubiquitinating frizzled receptors, thereby targeting these Wnt receptors for degradation...
  57. doi Cationic polystyrene nanosphere toxicity depends on cell-specific endocytic and mitochondrial injury pathways
    Tian Xia
    Division of NanoMedicine, Department of Medicine, University of California, Los Angeles, California 90095, USA
    ACS Nano 2:85-96. 2008
    ..Taken together, the results of this study demonstrate the importance of cell-specific uptake mechanisms and pathways that could lead to sensitivity or resistance to cationic particle toxicity...
  58. pmc HIV enters cells via endocytosis and dynamin-dependent fusion with endosomes
    Kosuke Miyauchi
    Institute of Human Virology and Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, MD 21201, USA
    Cell 137:433-44. 2009
    ..These findings imply that HIV-1 infects cells via endocytosis and envelope glycoprotein- and dynamin-dependent fusion with intracellular compartments.
  59. pmc Caveolin-1-dependent occludin endocytosis is required for TNF-induced tight junction regulation in vivo
    Amanda M Marchiando
    Department of Pathology, The University of Chicago, Chicago, IL 60637, USA
    J Cell Biol 189:111-26. 2010
    ..imaging and transgenic mice expressing fluorescent-tagged occludin and ZO-1 fusion proteins to link occludin endocytosis to TNF-induced tight junction regulation...
  60. ncbi VEGF controls endothelial-cell permeability by promoting the beta-arrestin-dependent endocytosis of VE-cadherin
    Julie Gavard
    Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, DHHS, Bethesda, MD 20892 4340, USA
    Nat Cell Biol 8:1223-34. 2006
    ..Here, we provide evidence of a novel signalling pathway by which VEGF stimulation promotes the rapid endocytosis of a key endothelial cell adhesion molecule, VE-cadherin, thereby disrupting the endothelial barrier function...
  61. ncbi Clathrin-mediated endocytosis of a lipid-raft-associated protein is mediated through a dual tyrosine motif
    Ruth Rollason
    Department of Biochemistry, University of Bristol, Bristol, BS8 1T, UK
    J Cell Sci 120:3850-8. 2007
  62. pmc Sortilin-mediated endocytosis determines levels of the frontotemporal dementia protein, progranulin
    Fenghua Hu
    Department of Neurology, Yale University School of Medicine, New Haven, CT 06536, USA
    Neuron 68:654-67. 2010
    ..5- to 5-fold. The 50% PGRN decrease causative in FTLD-TDP cases is mimicked in GRN+/⁻ mice, and is fully normalized by Sort1 ablation. Sortilin-mediated PGRN endocytosis is likely to play a central role in FTLD-TDP pathophysiology.
  63. doi Membrane vesicles as conveyors of immune responses
    Clotilde Thery
    Institut National de la Santé et de la Recherche Médicale U932, Institut Curie, 26 Rue d Ulm, Paris, 75005, France
    Nat Rev Immunol 9:581-93. 2009
    ..This Review focuses on the role of membrane vesicles, in particular exosomes, in the communication between immune cells, and between tumour and immune cells...
  64. ncbi Transduction of receptor signals by beta-arrestins
    Robert J Lefkowitz
    Howard Hughes Medical Institute, Durham, NC 27710, USA
    Science 308:512-7. 2005
    ..This mechanism regulates aspects of cell motility, chemotaxis, apoptosis, and likely other cellular functions through a rapidly expanding list of signaling pathways...
  65. doi Endocytosis at the nanoscale
    Irene Canton
    The Krebs Institute, The Centre for Membrane Interaction and Dynamics, The Sheffield Cancer Research Centre, and the Department of Biomedical Science, The University of Sheffield, Western Bank, Sheffield, S10 2TN, UK
    Chem Soc Rev 41:2718-39. 2012
    b>Endocytosis is a fundamental process in which eukaryotic cells internalise molecules and macromolecules via deformation of the membrane and generation of membrane-bound carriers...
  66. pmc Mycobacteria target DC-SIGN to suppress dendritic cell function
    Teunis B H Geijtenbeek
    Department of Molecular Cell Biology, Vrije Universiteit Medical Center Amsterdam, 1081 BT Amsterdam, Netherlands
    J Exp Med 197:7-17. 2003
    ..Moreover, we demonstrate that DC-SIGN has a broader pathogen recognition profile than previously shown, suggesting that DC-SIGN may represent a molecular target for clinical intervention in infections other than HIV-1...
  67. ncbi Clathrin-mediated constitutive endocytosis of PIN auxin efflux carriers in Arabidopsis
    Pankaj Dhonukshe
    Center for Plant Molecular Biology ZMBP, Auf der Morgenstelle 3, University of Tubingen, D 72076 Tubingen, Germany
    Curr Biol 17:520-7. 2007
    b>Endocytosis is an essential process by which eukaryotic cells internalize exogenous material or regulate signaling at the cell surface [1]...
  68. doi Role of the clathrin terminal domain in regulating coated pit dynamics revealed by small molecule inhibition
    Lisa von Kleist
    Institute of Chemistry and Biochemistry and Neurocure Cluster of Excellence, Freie Universitat Berlin, 14195 Berlin, Germany
    Cell 146:471-84. 2011
    Clathrin-mediated endocytosis (CME) regulates many cell physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission...
  69. doi Membrane phosphatidylserine regulates surface charge and protein localization
    Tony Yeung
    Division of Cell Biology, Hospital for Sick Children, Toronto M5G 1X8, Canada
    Science 319:210-3. 2008
    ..More strongly cationic proteins, normally associated with the plasma membrane, relocalized to endocytic compartments when the plasma membrane surface charge decreased on calcium influx...
  70. pmc Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells
    Anthony V Nicola
    Medical Virology Section, Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA
    J Virol 77:5324-32. 2003
    ..Here we report that a major pathway for HSV entry into the HeLa and CHO-K1 cell lines is dependent on endocytosis and exposure to a low pH...
  71. doi Endocytosis unplugged: multiple ways to enter the cell
    Sudha Kumari
    National Centre for Biological Science TIFR, Bellary Road, Bangalore 560 065, Karnataka, India
    Cell Res 20:256-75. 2010
    b>Endocytosis occurs at the cell surface and involves internalization of the plasma membrane (PM) along with its constituent membrane proteins and lipids...
  72. pmc HM1.24 is internalized from lipid rafts by clathrin-mediated endocytosis through interaction with alpha-adaptin
    Naoko Masuyama
    Division of Pharmaceutical Cell Biology, Graduate School of Pharmaceutical Sciences, Kyushu University, 3 1 1 Maidashi, Fukuoka 812 8582, Japan
    J Biol Chem 284:15927-41. 2009
    ..24 was internalized from lipid rafts on the cell surface by clathrin-mediated endocytosis. Interestingly, a non-canonical tyrosine-based motif, which contains two tyrosine residues, Tyr-6 and Tyr-8, ..
  73. pmc Advances in analysis of low signal-to-noise images link dynamin and AP2 to the functions of an endocytic checkpoint
    François Aguet
    Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA
    Dev Cell 26:279-91. 2013
    ..due to potential redundancy among EAPs and, as we show here, the plasticity and resilience of clathrin-mediated endocytosis (CME)...
  74. pmc Ultrafast endocytosis at mouse hippocampal synapses
    Shigeki Watanabe
    Department of Biology and Howard Hughes Medical Institute, University of Utah, Salt Lake City 84112, Utah, USA
    Nature 504:242-7. 2013
    ..the assembly of clathrin scaffolds or in approximately 1 s by the reversal of fusion pores via 'kiss-and-run' endocytosis. Here we use optogenetics to stimulate cultured hippocampal neurons with a single stimulus, rapidly freeze them ..
  75. pmc Hepatitis C virus induces CD81 and claudin-1 endocytosis
    Michelle J Farquhar
    Institute for Biomedical Research, University of Birmingham, Birmingham, UK
    J Virol 86:4305-16. 2012
    ..domains; however, CD81 lacks such motifs, leading several laboratories to suggest a limited role for CD81 endocytosis in HCV entry...
  76. pmc Agonist-induced PKC phosphorylation regulates GluK2 SUMOylation and kainate receptor endocytosis
    Filip A Konopacki
    Medical Research Council Centre for Synaptic Plasticity, University of Bristol, Bristol BS8 1TD, United Kingdom
    Proc Natl Acad Sci U S A 108:19772-7. 2011
    The surface expression and regulated endocytosis of kainate (KA) receptors (KARs) plays a critical role in neuronal function...
  77. pmc Clathrin mediates integrin endocytosis for focal adhesion disassembly in migrating cells
    Ellen J Ezratty
    Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA
    J Cell Biol 187:733-47. 2009
    ..To test whether endocytosis may be involved, we interfered with the function of clathrin or its adaptors autosomal recessive ..
  78. pmc Dynamin I phosphorylation by GSK3 controls activity-dependent bulk endocytosis of synaptic vesicles
    Emma L Clayton
    Centre for Integrative Physiology, George Square, University of Edinburgh, Edinburgh, Scotland, UK
    Nat Neurosci 13:845-51. 2010
    ..of expression with short hairpin RNA, we found that GSK3 was specifically required for activity-dependent bulk endocytosis (ADBE) but not clathrin-mediated endocytosis...
  79. ncbi The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing
    W Jiang
    Laboratory of Molecular Immunology, Rockefeller University, New York, New York 10021, USA
    Nature 375:151-5. 1995
    ..to the macrophage mannose receptor and related receptors which are able to bind carbohydrates and mediate endocytosis. DEC-205 is rapidly taken up by means of coated pits and vesicles, and is delivered to a multivesicular ..
  80. pmc Activity-dependent ubiquitination of GluA1 mediates a distinct AMPA receptor endocytosis and sorting pathway
    Lindsay A Schwarz
    Neurobiology Section, Division of Biological Sciences, University of California, San Diego, La Jolla, California 92093, USA
    J Neurosci 30:16718-29. 2010
    ..that ubiquitination of integral membrane proteins is a common posttranslational modification used to mediate endocytosis and endocytic sorting of surface proteins in eukaryotic cells...
  81. pmc Lipid raft endocytosis and exosomal transport facilitate extracellular trafficking of annexin A2
    Mallika Valapala
    Department of Biomedical Sciences, University of North Texas Health Science Center, Fort Worth, Texas 76107, USA
    J Biol Chem 286:30911-25. 2011
    ..The Ca(2+)-dependent exosomal transport constitutes a novel pathway of extracellular transport of AnxA2...
  82. ncbi Distinct pathways of antigen uptake and intracellular routing in CD4 and CD8 T cell activation
    Sven Burgdorf
    Institute of Molecular Medicine and Experimental Immunology, Friedrich Wilhelms Universität, Bonn, Germany
    Science 316:612-6. 2007
    ..We demonstrated that APCs use distinct endocytosis mechanisms to simultaneously introduce soluble antigen into separate intracellular compartments, which were ..
  83. doi Endocytosis, recycling, and regulated exocytosis of glucose transporter 4
    Kevin Foley
    Cell Biology Program, The Hospital for Sick Children, Toronto, Ontario M4G 1X8, Canada
    Biochemistry 50:3048-61. 2011
  84. pmc Generation of cell polarity in plants links endocytosis, auxin distribution and cell fate decisions
    Pankaj Dhonukshe
    Department of Biology, Faculty of Science, Utrecht University, Padualaan 8, 3584 CH, Utrecht, The Netherlands
    Nature 456:962-6. 2008
    ..Here we identify an endocytosis-dependent mechanism of PIN polarity generation and analyse its developmental implications...
  85. ncbi Cell-penetrating peptides: mechanism and kinetics of cargo delivery
    Matjaz Zorko
    Institute of Biochemistry, Medical Faculty, University of Ljubljana, Vrazov trg 2, SLO 1000, Ljubljana, Slovenia
    Adv Drug Deliv Rev 57:529-45. 2005
    ..receptor and energy independent although, in certain cases, translocation can be partially mediated by endocytosis. Cargoes that are successfully internalized by CPPs range from small molecules to proteins and supramolecular ..
  86. pmc Direct dynamin-actin interactions regulate the actin cytoskeleton
    Changkyu Gu
    Nephrology Division, Department of Medicine, Harvard Medical School and Massachusetts General Hospital, Charlestown, MA 02129, USA
    EMBO J 29:3593-606. 2010
    The large GTPase dynamin assembles into higher order structures that are thought to promote endocytosis. Dynamin also regulates the actin cytoskeleton through an unknown, GTPase-dependent mechanism...
  87. ncbi Curvature of clathrin-coated pits driven by epsin
    Marijn G J Ford
    MRC Laboratory of Molecular Biology, Cambridge, UK
    Nature 419:361-6. 2002
    Clathrin-mediated endocytosis involves cargo selection and membrane budding into vesicles with the aid of a protein coat...
  88. doi Systems survey of endocytosis by multiparametric image analysis
    Claudio Collinet
    Max Planck Institute for Molecular Cell Biology and Genetics, Germany
    Nature 464:243-9. 2010
    b>Endocytosis is a complex process fulfilling many cellular and developmental functions...
  89. ncbi Imaging actin and dynamin recruitment during invagination of single clathrin-coated pits
    Christien J Merrifield
    Vollum Institute L 474, Oregon Health and Sciences University, 3181 SW Sam Jackson Park Rd, Portland, OR 97201, USA
    Nat Cell Biol 4:691-8. 2002
    As a final step in endocytosis, clathrin-coated pits must separate from the plasma membrane and move into the cytosol as a coated vesicle...
  90. pmc Beyond desensitization: physiological relevance of arrestin-dependent signaling
    Louis M Luttrell
    Department of Medicine, Medical University of South Carolina, USA
    Pharmacol Rev 62:305-30. 2010
    ..arrestin-bound effectors serve to modulate G protein signaling, degrading second messengers and regulating endocytosis and trafficking, other signals seem to extend beyond the receptor-arrestin complex to regulate such processes ..
  91. doi Regulation of GPCRs by endocytic membrane trafficking and its potential implications
    Aylin C Hanyaloglu
    Institute of Reproductive Biology and Development, Imperial College London, Hammersmith Campus, London, United Kingdom
    Annu Rev Pharmacol Toxicol 48:537-68. 2008
    The endocytic pathway tightly controls the activity of G protein-coupled receptors (GPCRs). Ligand-induced endocytosis can drive receptors into divergent lysosomal and recycling pathways, producing essentially opposite effects on the ..
  92. pmc The early endosome: a busy sorting station for proteins at the crossroads
    Marko Jovic
    Department of Biochemistry and Molecular Biology and Eppley Cancer Center, University of Nebraska Medical Center, Omaha, Nebraska 68198 5870, USA
    Histol Histopathol 25:99-112. 2010
    b>Endocytosis marks the entry of internalized receptors into the complex network of endocytic trafficking pathways. Endocytic vesicles are rapidly targeted to a distinct membrane-bound endocytic organelle referred to as the early endosome...
  93. pmc Multiple mechanisms collectively regulate clathrin-mediated endocytosis of the epidermal growth factor receptor
    Lai Kuan Goh
    Department of Pharmacology, School of Medicine, University of Colorado Denver, Aurora, CO 80045, USA
    J Cell Biol 189:871-83. 2010
    b>Endocytosis of the epidermal growth factor receptor (EGFR) is important for the regulation of EGFR signaling...
  94. ncbi Myristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II
    Annie Quan
    Cell Signaling Unit, Children s Medical Research Institute, University of Sydney, Sydney, NSW 2145, Australia
    Mol Pharmacol 72:1425-39. 2007
    Dynamin is a GTPase enzyme involved in membrane constriction and fission during endocytosis. Phospholipid binding via its pleckstrin homology domain maximally stimulates dynamin activity...
  95. ncbi The Cbl interactome and its functions
    Mirko H H Schmidt
    Institute for Biochemistry II, Goethe University Medical School, Theodor Stern Kai 7, D 60590 Frankfurt am Main, Germany
    Nat Rev Mol Cell Biol 6:907-18. 2005
    ..Dysregulation of spatial or temporal constraints in the Cbl interactome results in the development of human pathologies such as immune diseases, diabetes and cancer...
  96. doi TRAIL resistance of breast cancer cells is associated with constitutive endocytosis of death receptors 4 and 5
    Yaqin Zhang
    Division of Therapeutic Proteins, Office of Biotechnology Products, Center for Drug Evaluation and Research, Food and Drug Administration, 29 Lincoln Drive, Bethesda, MD 20892, USA
    Mol Cancer Res 6:1861-71. 2008
    ..Furthermore, inhibition of endocytosis by pharmacologic inhibitors or disruption of clathrin-dependent endocytosis signaling components (adaptor ..
  97. ncbi Endocytosis of major histocompatibility complex class I molecules is induced by the HIV-1 Nef protein
    O Schwartz
    Laboratoire Rétrovirus et Transfert Génétique, Unité de Recherche Associée CNRS 1157, Paris, France
    Nat Med 2:338-42. 1996
    ..Nef is not necessary for viral replication in vitro and stimulates the endocytosis of CD4...
  98. doi Roles for actin assembly in endocytosis
    Olivia L Mooren
    Department of Cell Biology and Physiology, Washington University School of Medicine, Saint Louis, Missouri 63110, USA
    Annu Rev Biochem 81:661-86. 2012
    b>Endocytosis includes a number of processes by which cells internalize segments of their plasma membrane, enclosing a wide variety of material from outside the cell...
  99. doi Endocytosis and autophagy: Shared machinery for degradation
    Christopher A Lamb
    London Research Institute, Cancer Research UK, London, UK
    Bioessays 35:34-45. 2013
    ..Editor's suggested further reading in BioEssays The evolution of dynamin to regulate clathrin-mediated endocytosis Abstract.
  100. doi LRRK2 controls an EndoA phosphorylation cycle in synaptic endocytosis
    Samer Matta
    VIB Center for the Biology of Disease, 3000 Leuven, Belgium
    Neuron 75:1008-21. 2012
    ..mutants and Parkinson-related LRRK2(G2019S) gain-of-kinase function, we show that LRRK2 affects synaptic endocytosis by phosphorylating EndoA at S75, a residue in the BAR domain...
  101. pmc Exosome uptake depends on ERK1/2-heat shock protein 27 signaling and lipid Raft-mediated endocytosis negatively regulated by caveolin-1
    Katrin J Svensson
    Department of Clinical Sciences, Section of Oncology, Lund University, SE 22184 Lund, Sweden
    J Biol Chem 288:17713-24. 2013
    ..internalization of exosomes derived from glioblastoma (GBM) cells involves nonclassical, lipid raft-dependent endocytosis. Importantly, we show that the lipid raft-associated protein caveolin-1 (CAV1), in analogy with its previously ..

Research Grants72

  1. Novel TIRF microscopy analyzing trafficking & signaling at the cell cortex
    Derek Toomre; Fiscal Year: 2009
    ..Consistent with the recovery act's goal, this funding will provide full-time summer employment for 4 undergraduate students and accelerate scientific achievement of the parent DP2 award...
  2. Mapping for Specialized Domains for FCeRI Signaling &Internalization
    Bridget S Wilson; Fiscal Year: 2013
    ..Molecular segregation can occur within islands, facilitating signal transduction and at least two pathways of endocytosis. Cytoskeletal elements, including actin-based corrals and cortical cytoskeleton networks, restrict receptor ..
    Melanie H Cobb; Fiscal Year: 2012
    ..We have identified a dozen WNK1 targets that include proteins that directly activate ion transporters;regulate endocytosis, exocytosis and vesicular trafficking;and are components of signal transduction pathways essential for growth ..
  4. T cell regulation by N-glycosylation
    Michael Demetriou; Fiscal Year: 2013
    ..occupied N-X-S/T sites) cooperate to regulate binding to galectins and thereby the distribution, clustering and endocytosis of surface glycoproteins in a predictable manner...
  5. Tissue-specific delivery of probes by control of membrane trafficking of endoprot
    Blake Peterson; Fiscal Year: 2010
    ..to motifs that bind drugs or molecular probes, they can shuttle these compounds into cells and tissues via endocytosis. These unique biological activities result from functional mimicry of free cholesterol, a key component of ..
  6. Antibody-mediated Gene Therapy for the Treatment of Cancer
    TRACY RUTH DANIELS-WELLS; Fiscal Year: 2013
    ..or biotinylated lentiviral vectors in order to deliver a toxic gene into malignant B cells by receptor-mediated endocytosis. The use of two independent and non-exclusive gene therapy strategies is proposed in this application...
  7. Combined BAC Transgenic and Knock-Out Mouse Model of Lowe Syndrome Nephropathy
    Robert L Nussbaum; Fiscal Year: 2013
    ..has been proposed that derangements in phosphatidylinositol(4,5)P2 metabolism in LOCR causes defective receptor endocytosis, such as apical trafficking or recycling of megalin, a protein responsible for reabsorbing LMW proteins in the ..
  8. Role of Endocytosis and Vesicular Trafficking in Podocyte Health and Filter Integ
    Puneet Garg; Fiscal Year: 2012
    ..Recent investigations have suggested that the podocytes are very active in the process of endocytosis that routinely remove proteins like immunoglobulin that accumulate at the filtrations barrier...
  9. Brain Delivery of Nanoformulated Superoxide Dismutase
    Erin G Rosenbaugh; Fiscal Year: 2013
    ..This project will test the hypothesis that SOD-nano is transported into central neurons via active endocytosis and that following peripheral administration is taken up by neurons in AngII-sensitive brain regions which lack ..
    Sanjay Awasthi; Fiscal Year: 2011
    ..actin-cytoskeletal regulation and cell migration (cancer cell invasion and metastasis), receptor-ligand-pair endocytosis for signal termination, radiant and oxidant stress-responses, and apoptosis...
    Frances M Brodsky; Fiscal Year: 2013
    ..CCVs implement the fundamental cellular membrane traffic pathways of endocytosis and lysosome biogenesis...
  12. The biology of blood-testis barrier dynamics
    C Yan Cheng; Fiscal Year: 2013
    ..that cytokines and testosterone mediate their opposing effects on the BTB via their differential actions on endocytosis, recycling, endosome-mediated intracellular degradation and transcytosis/relocation of integral membrane ..
  13. Identifying Key Structural Elements in the Activation of the Arp2/3 Complex
    Patricia A Khuu; Fiscal Year: 2013
    ..The complex is essential for changes to cell shape associated with specific processes, such as endocytosis in yeast, and is required for the formation of actin-based structures, such as actin patches and lamellipodia...
  14. Regulation of Signaling Receptor Activity by LRP-1
    Steven L Gonias; Fiscal Year: 2013
    ..Low density lipoprotein receptor-related protein-1 (LRP-1) is a transmembrane receptor that mediates the endocytosis of over forty distinct ligands and other plasma membrane proteins...
    DAVID ANTONETTI; Fiscal Year: 2013
    ..that mutation of occludin to prevent phosphorylation, blocks VEGF-induced permeability and occludin endocytosis. Furthermore, preliminary data demonstrate that TNF alters the tight junction complex by reducing the tight ..
  16. Regulation of alpha2A-adrenergic receptor signaling and trafficking by spinophili
    Qin Wang; Fiscal Year: 2013
    ..Specific Aim 1 will determine the role of spinophilin in regulation of agonist-induced ?2AAR endocytosis in native neurons...
  17. Neuronal mRNA PET Imaging
    Eric Wickstrom; Fiscal Year: 2010
    ..Other laboratories have demonstrated that PNA conjugates can cross the blood-brain barrier. For endocytosis of mRNA imaging agents specifically into neuronal cells, we need to use a characteristic neuronal receptor...
  18. ClC-3 Chloride Ion Channels in Vascular Smooth Muscle
    Fred Lamb; Fiscal Year: 2009
    ..in response to cytokines (TNF[unreadable] and IL-1[unreadable]) occurs in early endosomes following receptor endocytosis. Endosomal O2-" production by the Nox1 NADPH oxidase (originally termed the "mitogenic oxidase" because it is ..
    Stephan Wilkens; Fiscal Year: 2013
    ..In eukaryotic cells, these processes include receptor mediated endocytosis, protein trafficking, pH maintenance, storage of metabolites and neurotransmitter release...
  20. Disease-related defects in dendritic cell processing of bacterial antigens
    Michael S Marks; Fiscal Year: 2012
    ..or AP-3 are required for (a) optimal DC presentation of antigens following phagocytosis or other forms of endocytosis in vitro and (b) T cell immune responses upon challenge with a bacterial pathogen (L. monocytogenes) in vivo. 2...
  21. Extracellular Matrix Remodeling During Aging
    JANE M SOTTILE; Fiscal Year: 2010
    ..Our data show that FN matrix turnover occurs through endocytosis and intracellular degradation, and is regulated by both caveolin-1 and 21 integrins...
  22. Liver Cell Membrane Protein-Expression and Function
    Allan W Wolkoff; Fiscal Year: 2012
    ..regulation of expression and trafficking of connexins (Project 2), the identification of trafficking steps in endocytosis mutants (Project 3), and mechanisms of trafficking to autophagic vacuoles and lysosomes (Project 4)...
  23. Laser Spinning Disc Confocal System for live-cell and live-organism microscopy
    Martha C Soto; Fiscal Year: 2012
    ..Studies of endocytosis in the Grant lab have identified key mechanisms for the regulation of receptor trafficking, identifying many ..
  24. SK channels: Roles and mechanisms for dendritic excitability and plasticity
    James G Maylie; Fiscal Year: 2012
    ..3) SK2 channels in the PSD undergo basal recycling through dynamin-dependent endocytosis and recycling endosomes...
  25. Biochemistry and Cell Biology of CHC22 Clathrin
    Frances M Brodsky; Fiscal Year: 2013
    ..well studied and forms the outer layer of clathrin-coated vesicles, which sort cargo during receptor-mediated endocytosis from the plasma membrane (PM), endosomal traffic, and organelle biogenesis from the trans-Golgi network (TGN)...
  26. Mechanisms of Hormonal Regulation of the Sodium Chloride Cotransporter
    Robert S Hoover; Fiscal Year: 2013
    ..signal-Regulated Kinases 1 and 2 Mitogen-Activated Protein Kinases) pathway in the ubiquitination and endocytosis of NCC...
    MARK A MC NIVEN; Fiscal Year: 2013
    ..associates with the actin cytoskeleton and specific adaptor proteins at both the PM to regulate receptor endocytosis and the TGN to mediate trafficking of nascent hydrolases to the lysosome...
  28. Roles for Phospholipase D2 in metabolism and cardiovascular homeostasis
    ROCHELLE NELSON; Fiscal Year: 2013
    ..PLD2 has been proposed by my sponsor's lab to regulate endocytosis and signaling efficiency of the Angiotensin II receptor, and a PLD2 polymorphism in humans correlates with ..
  29. Development and testing of polyvalent anthrax toxin inhibitors
    JEREMY S MOGRIDGE; Fiscal Year: 2012
    ..Heptamerization allows binding of the enzymatic toxin components, edema factor and lethal factor, and triggers endocytosis of these complexes and intoxication of the cells...
  30. Disordered Cellular Cholesterol Movement, Cell Death, and Multisystem Disease
    STEPHEN DEAN TURLEY; Fiscal Year: 2013
    ..One of the most poorly understood areas of cell biology involves the role of receptor-mediated and bulk-phase endocytosis of cholesterol, coupled to ApoE or ApoB100 containing lipoproteins, in plasma membrane sterol turnover and ..
    Nigel W Bunnett; Fiscal Year: 2010
    ..Aim 1 will define the molecular mechanism of SP-stimulated endocytosis and intracellular trafficking of the NK1R...
  32. 2011 Cell-Cell Fusion Gordon Research Conference and Seminar
    NANCY RYAN GRAY; Fiscal Year: 2011
    ..as the relationship between cell fusion and the larger fields of membrane fusion and fission during exocytosis, endocytosis, viral entry, and cytokinesis...
  33. Exocytosis and Coupled Endocytosis in Neuroendocrine Cells
    JOHN DAVID CASTLE; Fiscal Year: 2010
    The long-range objective is to understand the mechanisms involved in coupled exocytosis and endocytosis in neuroendocrine cells...
  34. Pathophysiology and potential therapy for a childhood neurodegenerative disease
    Marquis T Walker; Fiscal Year: 2011
    ..Aim one is to test the hypothesis that the hematopoietic phagocytes in the TRPML1-/- mice are defective in endocytosis and lysosomal dependent degradation...
  35. HCV in Alcoholics
    Jack R Wands; Fiscal Year: 2011
    ..effect on DCs with respect to cell surface markers, cytokine profiles, antigen presentation ability, and endocytosis capacity...
  36. Novel Immunodeficiency caused by TFRC Mutation
    RAIF SALIM GEHA; Fiscal Year: 2013
    ..surface TfR1 expression and have defective transferrin uptake, indicating that the Y20H mutation impairs TfR1 endocytosis and TfR1-dependent intracellular import of iron-laden holotransferrin...
  37. Endocannabinoid Intracellular Transporters
    Dale G Deutsch; Fiscal Year: 2010
    ..experiments will also be conducted to show that two alternative mechanisms (simple diffusion or endocytosis) are less likely...
  38. Roles of Phospholipase D in AT1 Receptor Endocytosis
    Guangwei Du; Fiscal Year: 2010
    ..b>Endocytosis of the Ang ll-bound receptor not only desensitizes AT1R signaling from the cell surface but also activates ..
    George M Carman; Fiscal Year: 2013
    ..that triggers phospholipid synthesis gene expression, membrane expansion, vesicular trafficking, secretion, and endocytosis. Biochemical and genetic studies to establish the roles of PAP in lipid metabolism became possible by our ..
  40. Receptor-mediated Endocytosis-Mechanism and Function
    Frederick R Maxfield; Fiscal Year: 2013
    ..In Aim V the Interplay between sterols and membrane traffic will be explored. In particular, the function of tubules that emanate from late endosomes In response to transient sterol loading will be studied. ..
  41. Cholesterol and Sphingolipid Perturbation of Membrane Traffic in Human Disease
    Andrew H Limper; Fiscal Year: 2011
    ..Under Aim 2, we will study the effects of SLs and cholesterol on endocytosis, recycling, and function of WT- and ?F508-CFTR since these lipids can significantly affect membrane transport ..
  42. Cellular and molecular mechanisms of gp120 neurotoxicity
    Italo Mocchetti; Fiscal Year: 2012
    ..Preliminary evidence has shown that the chemokine receptor CXCR4 promotes endocytosis of T-tropic gp120 into neurons. Endocytosis of gp120 is followed by axonal transport through microtubules...
  43. The role of caveolin 1 in intestinal lipid processing and transport
    JESSICA OTIS; Fiscal Year: 2013
    ..In Specific Aim 2 caveolar endocytosis will be visualized and disrupted...
  44. HS1 Function in Dendritic Cells
    Janis K Burkhardt; Fiscal Year: 2011
    ..We hypothesize that HS1 works together with WASp and dynamin II in DCs, to control receptor-mediated endocytosis and podosome- dependent migration to lymphoid organs. To test this hypothesis, we will carry out three Aims...
  45. Cardiac function and PIP2
    Donald W Hilgemann; Fiscal Year: 2013
    ..Our overall goal is a better understanding of the `life-time'and endocytosis of NCX1...
  46. SK2 Channels as Novel Neuroprotective Targets Against Cerebral Ischemia
    Paco S Herson; Fiscal Year: 2012
    ..3. CA/CPR-induced ischemia causes PKA phosphorylation of spine SK2 channels, inducing channel endocytosis. Expression of PKA-immune SK2 channels will normalize the SK2 and NMDAr contributions to the EPSP, the NMDAr-..
  47. Cellular Mechanisms of Amniotic Fluid Volume Regulation
    Cecilia Y Cheung; Fiscal Year: 2013
    ..a c-Src signaling pathway leading to downstream activation of cavoelin-1 and dynamin-2 as required for caveolar endocytosis and transcytosis...
  48. Mechanisms Regulating Clathrin-Mediated Endocytosis
    Sandra L Schmid; Fiscal Year: 2013
    DESCRIPTION (provided by applicant): Clathrin-mediated endocytosis (CME) occurs via the assembly of clathrin-coated pits (CCPs), specialized microdomains at the plasma membrane that undergo progressive maturation and eventually ..
  49. The Role of Intracellular Metabotropic Glutamate Receptor 5 at the Synapse
    CAROLYN ANN PURGERT; Fiscal Year: 2012
    ..lead to local protein synthesis at the synapse and AMPA receptor (an excitatory ionotropic glutamate receptor) endocytosis as well as transcription of synaptic plasticity genes;however, the contributions of cell surface mGluR5 versus ..
  50. Biomimetic Microsystem for High Throughput Evaluation of Engineered Nanomaterials
    Robert M Worden; Fiscal Year: 2010
    ..LPS bind to receptor proteins on cell membranes and trigger cellular uptake by endocytosis, and quinones can trigger oxidation-reduction reactions, including production of reactive oxygen species by ..
  51. Molecular Mechanisms of Albumin Trafficking in Podocytes
    Judith T Blaine; Fiscal Year: 2013
    ..Podocytes can take up albumin but the mechanisms of endocytosis in podocytes are not known...
  52. Regulation of AKAP79 Postsynaptic Targeting and Signaling
    MARK L DELL'ACQUA; Fiscal Year: 2013
    ..During LTD, the Ca2+-activated phosphatase calcineurin (CaN) dephosphorylates S845 and removes GluA1 via endocytosis. PKA and CaN are targeted to GluA1 through binding to A-kinase anchoring protein (AKAP) 79/150 (human79/..
  53. Spatiotemporal Regulation of Membrane Raft Trafficking in Virus Activated Cells
    Tione Buranda; Fiscal Year: 2012
    ..Membrane rafts on the cell surface are often the originating point of cell signaling, adhesion and endocytosis. Viruses use signaling elements that are bundled in membrane rafts and co-opt the cellular endocytic machinery ..
  54. Specification of actomyosin function in the cell
    Matthew Lord; Fiscal Year: 2013
    ..that adapt them for their given roles? Presently, we are focused on how myosin-I is directed to sites of endocytosis (actin patches), how myosin-II functions at contractile rings during cytokinesis, and how myosin-V is specified ..
  55. Interaction of Sindbis Virus with Cellular Processes
    ILYA V FROLOV; Fiscal Year: 2010
    ..that the first type of nsP3-specific complexes is initially formed on the cellular membrane and, following endocytosis, remains associated with endosome-like, membrane-containing organelles...
    Nigel W Bunnett; Fiscal Year: 2010
    ..The role of arrestin and ubiquitin in endocytosis and lysosomal trafficking of PAR2, and the role of rabl 1a in mobilization of Golgi PAR2 will be studied in ..
  57. Endogenous Hemoglobin And Heme Detoxification After Intracerebral Hemorrhage
    Xiurong Zhao; Fiscal Year: 2013
    ..Clearance of cell-free Hb/heme through phagocytosis/endocytosis is an essential function of microglia/macrophages in anti-inflammatory response...
  58. PI-3 kinase effectors in insulin-responsive systems
    Silvia Corvera; Fiscal Year: 2010
    ..elegans. Using a strain that reveals endocytosis defects, we have identified a highly conserved, previously uncharacterized protein essential for endocytosis, ..
    R Dyche Mullins; Fiscal Year: 2013
    ..powering locomotion, such networks also underlie other fundamental cellular processes, including: phagocytosis, endocytosis, movement of intracellular pathogens, and healing of membrane ruptures...
  60. Structural Studies of Alphavirus Membrane Fusion In Situ
    Wei Zhang; Fiscal Year: 2010
    ..These viruses invade the host cells through the receptor-mediated endocytosis pathway...
  61. Uncovering the Regulatory Role of gH/gL in HSV Fusion
    Roselyn J Eisenberg; Fiscal Year: 2013
    ..gB-gH/gL complex formation during HSV entry when it occurs by direct fusion at the plasma membrane or by endocytosis. Our work on HSV entry and fusion has strong clinical significance, as each step is a potential target for ..
  62. Membrane Receptors Regulating Megakaryocyte Endocytosis of Factor V from Plasma
    BETH BOUCHARD; Fiscal Year: 2012
    ..demonstrated that the entire pool of the platelet-derived procofactor, factor V, originates from plasma through endocytosis of factor V by platelet precursors, megakaryocytes, via a two receptor system consisting of a specific, as of ..
    Cristina Artalejo; Fiscal Year: 2004
    ..cells release neurotransmitters/ hormones by exocytosis followed by recovery of vesicular membrane by endocytosis. For many years, the focus of the P1's laboratory has been to understand the processes underlying the secretory ..
    Vincent Agnello; Fiscal Year: 2001
    ..We have also established that the LDL receptor mediates endocytosis of HCV and other members of the Flaviviridae family and that the apolipoprotein E epsilon2 allele in HCV ..
  65. The Role of Arf GTPases in Endocytosis and Postendocytic Transport
    James E Casanova; Fiscal Year: 2010
    ..of clathrin-coated vesicles in the Golgi apparatus and on endosomes, but their role in clathrin- mediated endocytosis at the plasma membrane is poorly understood...
    CAROLINE ENNS; Fiscal Year: 2002
    ..Transferrin, in turn, is taken up into cells by receptor- meditated endocytosis through binding to the transferrin receptor on the cell surface...
  67. Role of Myosin VI in CFTR Endocytosis in the Intestine
    Nadia Ameen; Fiscal Year: 2007
    ..years are a natural extension of work proposed in the K08 application to examine the role that actin-dependent endocytosis plays in regulating surface pools of CFTR in the small intestine following cyclic nucleotide activation...
  68. Roles of ubiquitin protein ligases in endocytosis
    Linda Hicke; Fiscal Year: 2005
    ..These ligases play multiple roles in endocytosis: they are required to ubiquitinate endocytic cargo, they modify trans-acting components of the endocytic ..
    Wayne Lencer; Fiscal Year: 1993
    The scientific goal of this proposal is to elucidate the role of endocytosis and transepithelial transport in the Cholera toxin (CT) induced activation of adenylate cyclase in intestinal epithelial cells...
  70. Role of dynamin-auxilin interactions in endocytosis
    Sanja Sever; Fiscal Year: 2009
    Clathrin-coated vesicle formation at the plasma membrane underlies the process of clathrin-mediated endocytosis by which the cell internalizes receptors, transmembrane channels, transporters, hormones growth factors and nutrients...
  71. Secretion-associated dynamic of the plasma membrane
    Arun Anantharam; Fiscal Year: 2010
    ..nature of changes in plasma membrane morphology and biochemistry during the coupled processes of exocytosis and endocytosis. When triggered by Ca2+, neuroendocrine cells release biogenic amines and neuropeptides into the extracellular ..
    Dahn Clemens; Fiscal Year: 1999
    ..hepatic dysfunctions observed in animals chronically fed ethanol is a impairment in receptor-mediated endocytosis. Receptor-mediated endocytosis is responsible for the uptake and degradation of many biologically important ..