respiratory burst

Summary

Summary: A large increase in oxygen uptake by neutrophils and most types of tissue macrophages through activation of an NADPH-cytochrome b-dependent oxidase that reduces oxygen to a superoxide. Individuals with an inherited defect in which the oxidase that reduces oxygen to superoxide is decreased or absent (GRANULOMATOUS DISEASE, CHRONIC) often die as a result of recurrent bacterial infections.

Top Publications

  1. ncbi Exercise-induced alterations in neutrophil degranulation and respiratory burst activity: possible mechanisms of action
    Jonathan M Peake
    School of Human Movement Studies, University of Queensland, St Lucia, QLD 4072, Australia
    Exerc Immunol Rev 8:49-100. 2002
  2. ncbi Respiratory burst oxidases: the engines of ROS signaling
    Nobuhiro Suzuki
    Department of Biological Sciences, College of Arts and Sciences, University of North Texas, Denton, TX 76203, USA
    Curr Opin Plant Biol 14:691-9. 2011
  3. ncbi K+ channels and the microglial respiratory burst
    R Khanna
    Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada
    Am J Physiol Cell Physiol 280:C796-806. 2001
  4. ncbi Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines
    Irundika H K Dias
    School of Life and Health Sciences, Aston University, Birmingham, UK
    J Clin Periodontol 38:1-7. 2011
  5. pmc Clinical protection from falciparum malaria correlates with neutrophil respiratory bursts induced by merozoites opsonized with human serum antibodies
    Charlotte Joos
    Institut Pasteur de Dakar, Unite d immunologie, Dakar, Senegal
    PLoS ONE 5:e9871. 2010
  6. pmc Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst
    I Scott Ramsey
    Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, and Department of Cardiovascular Research, Manton Center for Orphan Disease, Children s Hospital, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:7642-7. 2009
  7. ncbi Reactive oxygen species and cell signaling: respiratory burst in macrophage signaling
    Henry Jay Forman
    Department of Environmental Health Sciences, School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama 35294
    Am J Respir Crit Care Med 166:S4-8. 2002
  8. pmc Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene
    Malin Hultqvist
    Section for Medical Inflammation Research, Lund University, SE 22184 Lund, Sweden
    Proc Natl Acad Sci U S A 101:12646-51. 2004
  9. ncbi Iron chelation via deferoxamine exacerbates experimental salmonellosis via inhibition of the nicotinamide adenine dinucleotide phosphate oxidase-dependent respiratory burst
    Helen L Collins
    Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany
    J Immunol 168:3458-63. 2002
  10. ncbi Measurement of respiratory burst products generated by professional phagocytes
    Claes Dahlgren
    Department of Rheumatology and Inflammation Research, University of Goteborg, Goteborg, Sweden
    Methods Mol Biol 412:349-63. 2007

Detail Information

Publications311 found, 100 shown here

  1. ncbi Exercise-induced alterations in neutrophil degranulation and respiratory burst activity: possible mechanisms of action
    Jonathan M Peake
    School of Human Movement Studies, University of Queensland, St Lucia, QLD 4072, Australia
    Exerc Immunol Rev 8:49-100. 2002
    ..Moderate intensity exercise may enhance neutrophil respiratory burst activity, possibly through increases in the concentrations of growth hormone and the inflammatory cytokine ..
  2. ncbi Respiratory burst oxidases: the engines of ROS signaling
    Nobuhiro Suzuki
    Department of Biological Sciences, College of Arts and Sciences, University of North Texas, Denton, TX 76203, USA
    Curr Opin Plant Biol 14:691-9. 2011
    ..Recent studies identified respiratory burst oxidase homologues (RBOHs) as key signaling nodes in the ROS gene network of plants integrating a multitude ..
  3. ncbi K+ channels and the microglial respiratory burst
    R Khanna
    Division of Cellular and Molecular Biology, Toronto Western Research Institute, University Health Network, Toronto, Ontario, Canada
    Am J Physiol Cell Physiol 280:C796-806. 2001
    Microglial activation following central nervous system damage or disease often culminates in a respiratory burst that is necessary for antimicrobial function, but, paradoxically, can damage bystander cells...
  4. ncbi Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines
    Irundika H K Dias
    School of Life and Health Sciences, Aston University, Birmingham, UK
    J Clin Periodontol 38:1-7. 2011
    ..To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines...
  5. pmc Clinical protection from falciparum malaria correlates with neutrophil respiratory bursts induced by merozoites opsonized with human serum antibodies
    Charlotte Joos
    Institut Pasteur de Dakar, Unite d immunologie, Dakar, Senegal
    PLoS ONE 5:e9871. 2010
    ..Repeated blood stage infections induce antibodies considered to be the main arbiters of protection from pathology, but their essential functions have remained speculative...
  6. pmc Hv1 proton channels are required for high-level NADPH oxidase-dependent superoxide production during the phagocyte respiratory burst
    I Scott Ramsey
    Howard Hughes Medical Institute, Department of Neurobiology, Harvard Medical School, and Department of Cardiovascular Research, Manton Center for Orphan Disease, Children s Hospital, 320 Longwood Avenue, Boston, MA 02115, USA
    Proc Natl Acad Sci U S A 106:7642-7. 2009
    Granulocytes generate a "respiratory burst" of NADPH oxidase-dependent superoxide anion (O(2)(-*)) production that is required for efficient clearance of bacterial pathogens...
  7. ncbi Reactive oxygen species and cell signaling: respiratory burst in macrophage signaling
    Henry Jay Forman
    Department of Environmental Health Sciences, School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama 35294
    Am J Respir Crit Care Med 166:S4-8. 2002
    ..The rapid reaction of thiols with H2O2 when in their unprotonated state would provide a potential mechanism for the specificity that is necessary for physiologic cell signaling...
  8. pmc Enhanced autoimmunity, arthritis, and encephalomyelitis in mice with a reduced oxidative burst due to a mutation in the Ncf1 gene
    Malin Hultqvist
    Section for Medical Inflammation Research, Lund University, SE 22184 Lund, Sweden
    Proc Natl Acad Sci U S A 101:12646-51. 2004
    ....
  9. ncbi Iron chelation via deferoxamine exacerbates experimental salmonellosis via inhibition of the nicotinamide adenine dinucleotide phosphate oxidase-dependent respiratory burst
    Helen L Collins
    Department of Immunology, Max Planck Institute for Infection Biology, Berlin, Germany
    J Immunol 168:3458-63. 2002
    ..Thus, altering the equilibrium of intracellular Fe influences the course of infection to the benefit of the pathogen...
  10. ncbi Measurement of respiratory burst products generated by professional phagocytes
    Claes Dahlgren
    Department of Rheumatology and Inflammation Research, University of Goteborg, Goteborg, Sweden
    Methods Mol Biol 412:349-63. 2007
    ..of basic techniques to measure/quantify ROS generation/release by phagocytes during activation of the respiratory burst is of great importance, and a large number of techniques have been used for this purpose...
  11. ncbi The non-neuronal cholinergic system in peripheral blood cells: effects of nicotinic and muscarinic receptor antagonists on phagocytosis, respiratory burst and migration
    Stefanie Neumann
    Institute of Pathology, University Hospital, Johannes Gutenberg University, D 55101 Mainz, Germany
    Life Sci 80:2361-4. 2007
    ..peripheral blood to investigate content and synthesis of acetylcholine as well as phenotypic functions like respiratory burst, phagocytosis and migration. Mononuclear cells (T-cells and monocytes) contained 0...
  12. pmc The p47phox mouse knock-out model of chronic granulomatous disease
    S H Jackson
    Laboratory of Host Defenses, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892 1886, USA
    J Exp Med 182:751-8. 1995
    ..This model mirrors human CGD and confirms a critical role for the phagocyte NADPH oxidase in mammalian host defense...
  13. ncbi Structure and regulation of the neutrophil respiratory burst oxidase: comparison with nonphagocyte oxidases
    Mark T Quinn
    Department of Veterinary Molecular Biology, Montana State University, Bozeman 59717 3610, USA
    J Leukoc Biol 76:760-81. 2004
    ..Furthermore, relevant details about structural and functional features of various nonphagocyte oxidase proteins will be included for comparison...
  14. ncbi Sequential activation of class IB and class IA PI3K is important for the primed respiratory burst of human but not murine neutrophils
    Alison M Condliffe
    The Inositide Laboratory, Babraham Institute, Babraham Research Campus, Cambridge CB2 4AT, UK
    Blood 106:1432-40. 2005
    ..These results start to define which PI3K isoforms are responsible for modulating neutrophil responsiveness to infection and inflammation...
  15. ncbi Fibrillar beta-amyloid-stimulated intracellular signaling cascades require Vav for induction of respiratory burst and phagocytosis in monocytes and microglia
    Brandy Wilkinson
    Alzheimer Research Laboratory, Department of Neuroscience, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106, USA
    J Biol Chem 281:20842-50. 2006
    ....
  16. ncbi N-acetylcysteine reduces respiratory burst but augments neutrophil phagocytosis in intensive care unit patients
    A R Heller
    Department of Anesthesiology and Intensive Care Medicine, University Hospital Carl Gustav Carus, Dresden, Germany
    Crit Care Med 29:272-6. 2001
    ..To evaluate whether NAC affects host defense mechanisms in critically ill patients, thus predisposing to increased risk of infection, the current study focuses on neutrophil phagocytotic and burst activity after treatment with NAC...
  17. ncbi Cholesterol-rich membrane rafts and Lyn are involved in phagocytosis during Pseudomonas aeruginosa infection
    Shibichakravarthy Kannan
    Department of Biochemistry and Molecular Biology, University of North Dakota School of Medicine and Health Sciences, Grand Forks, ND 58203, USA
    J Immunol 180:2396-408. 2008
    ..Further analysis indicates that Lyn and raft components entered in phagosomes and late lysosomes. Finally, respiratory burst was dependent on Lyn and membrane rafts, as confirmed by small interfering RNA and dominant-negative ..
  18. pmc Measurement of oxidative burst in neutrophils
    Yu Chen
    Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA
    Methods Mol Biol 844:115-24. 2012
    ..The first method depends on the reduction of cytochrome c, which can be assessed by photometry, while the second method relies on changes in the fluorescence properties of dihydrorhodamine 123, which can be assessed by flow cytometry...
  19. ncbi Neutrophil activation by heme: implications for inflammatory processes
    Aurelio V Graca-Souza
    Departamento de Farmacologia, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Brazil
    Blood 99:4160-5. 2002
    ..Taken together, these results suggest a role for hemin as a proinflammatory agent able to induce polymorphonuclear neutrophil activation in situations of clinical relevance, such as hemolysis or hemoglobinemia...
  20. ncbi Inhibitory effect of stobadine on FMLP-induced chemiluminescence in human whole blood and isolated polymorphonuclear leukocytes
    Katarina Drabikova
    Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dubravska cesta 9, 841 04 Bratislava, Slovak Republic
    Luminescence 22:67-71. 2007
    ....
  21. pmc Gingipains from Porphyromonas gingivalis increase the chemotactic and respiratory burst-priming properties of the 77-amino-acid interleukin-8 variant
    Irundika H K Dias
    School of Life and Health Sciences, Aston University, Aston Triangle, Birmingham B4 7ET, United Kingdom
    Infect Immun 76:317-23. 2008
    ..IL-8(77aa) has low chemotactic and respiratory burst-inducing activity but is susceptible to cleavage by gingipains...
  22. ncbi Melatonin regulates the respiratory burst of human neutrophils and their depolarization
    C Pieri
    Center of Cytology, Gerontol Res Dept of I N R C A N Masera, Chieti, Italy
    J Pineal Res 24:43-9. 1998
    The effect of different doses of melatonin on the respiratory burst as well as on the membrane potential changes of human neutrophils stimulated with phorbol 12-myristate 13-acetate (PMA) was investigated...
  23. ncbi The phosphatidylinositol 3-kinase signaling pathway exerts protective effects during sepsis by controlling C5a-mediated activation of innate immune functions
    Christiane D Wrann
    Department of Trauma Surgery, Hannover Medical School, Carl Neuberg Strasse 1, 30625 Hannover, Germany
    J Immunol 178:5940-8. 2007
    ..Our data suggest that the PI3K/Akt signaling pathway controls various C5a-mediated effects on neutrophil and monocyte innate immunity and exerts an overall protective effect during experimental sepsis...
  24. ncbi FLS2: an LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis
    L Gomez-Gomez
    Friedrich Miescher Institut, Basel, Switzerland
    Mol Cell 5:1003-11. 2000
    ..FLS2 shares structural and functional homologies with known plant resistance genes and with components involved in the innate immune system of mammals and insects...
  25. ncbi Interleukin-8-induced priming of neutrophil oxidative burst requires sequential recruitment of NADPH oxidase components into lipid rafts
    Cecile Guichard
    Unité INSERM 683, Facultédemédecine Xavier BICHAT, BP 416, 75870 Paris Cedex 18, France
    J Biol Chem 280:37021-32. 2005
    ..Our findings indicate that IL-8-induced priming of the oxidative burst in response to fMLP involves a sequential assembly of the NADPH oxidase components in the lipid rafts of neutrophils...
  26. ncbi A highly sensitive fluorescent micro-assay of H2O2 release from activated human leukocytes using a dihydroxyphenoxazine derivative
    J G Mohanty
    MCP Hahnemann School of Medicine, Division of Pulmonary and Critical Care Medicine, Allegheny University of the Health Sciences, Philadelphia, PA 19102, USA
    J Immunol Methods 202:133-41. 1997
    ..The protocol described in this study using A6550 to measure H2O2 release from activated granulocytes can be easily adapted to other cell types which generate H2O2...
  27. ncbi Respiratory burst: role in signal transduction in alveolar macrophages
    Maureen R Gwinn
    Pathology and Physiology Research Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, West Virginia 26505, USA
    J Toxicol Environ Health B Crit Rev 9:27-39. 2006
    ..One major macrophage defense mechanism is respiratory burst, the production of reactive oxygen species (ROS)...
  28. pmc Fungal metabolite gliotoxin inhibits assembly of the human respiratory burst NADPH oxidase
    Shohko Tsunawaki
    Department of Infectious Diseases, National Research Institute for Child Health and Development, Setagaya, Tokyo 154 8567, Japan
    Infect Immun 72:3373-82. 2004
    ..These results suggest that A. fumigatus may confront PMN by inhibiting the assembly of the NADPH oxidase with its hyphal product, GT...
  29. ncbi Naturally appearing N-feruloylserotonin isomers suppress oxidative burst of human neutrophils at the protein kinase C level
    Rado Nosal
    Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Dubravska 9, 841 04 Bratislava, Slovak Republic
    Pharmacol Rep 63:790-8. 2011
    ..It should be further investigated for its pharmacological activity against oxidative stress in ischemia-reperfusion, inflammation and other pathological conditions...
  30. ncbi Neutrophil survival is markedly reduced by incubation with influenza virus and Streptococcus pneumoniae: role of respiratory burst
    G Engelich
    Department of Medicine and Pathology, Boston University School of Medicine, Massachusetts 02218, USA
    J Leukoc Biol 69:50-6. 2001
    ..pneumoniae alone. This increased respiratory burst activity contributed to the diminished neutrophil survival caused by IAV and S. pneumoniae...
  31. pmc C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis
    Adrian Schreiber
    Medical Faculty of the Charite, Department of Nephrology and Hypertension, Franz Volhard Clinic at the Max Delbrück Center for Molecular Medicine, HELIOS Klinikum Berlin, Berlin, Germany
    J Am Soc Nephrol 20:289-98. 2009
    ..This conditioned serum primed neutrophils for ANCA-induced respiratory burst; neutrophil C5aR blockade abrogated this priming, but C3aR blockade did not...
  32. ncbi Expression and function of Toll-like receptors in chicken heterophils
    Michael H Kogut
    USDA ARS, Southern Plains Agricultural Research Center, 2881 F and B Road, College Station, TX 77845, USA
    Dev Comp Immunol 29:791-807. 2005
    ..The results demonstrate the differential involvement of TLR-induced signals in the stimulation of transduction pathways that regulate the oxygen-dependent and -independent antimicrobial defense mechanisms of avian heterophils...
  33. pmc Voltage-gated proton channels find their dream job managing the respiratory burst in phagocytes
    Thomas E DeCoursey
    Department of Molecular Biophysics and Physiology, Rush University Medical Center, Chicago, Illinois, USA
    Physiology (Bethesda) 25:27-40. 2010
    ..The proton channel seems designed for efficient proton extrusion from cells. In phagocytes, it facilitates the production of reactive oxygen species by NADPH oxidase...
  34. ncbi Elucidation of molecular events leading to neutrophil apoptosis following phagocytosis: cross-talk between caspase 8, reactive oxygen species, and MAPK/ERK activation
    Bin Zhang
    Vascular Research Division, Department of Pathology, Brigham and Women s Hospital and Harvard Medical School, Boston, Massachusetts 02115, USA
    J Biol Chem 278:28443-54. 2003
    ....
  35. ncbi Myeloperoxidase: friend and foe
    Seymour J Klebanoff
    Department of Medicine, University of Washington School of Medicine, Seattle, WA 98195 7185, USA
    J Leukoc Biol 77:598-625. 2005
    ..MPO), released into the phagosome during the degranulation process, hydrogen peroxide (H2O2), formed by the respiratory burst and a halide, particularly chloride...
  36. ncbi The human NADPH oxidase: primary and secondary defects impairing the respiratory burst function and the microbicidal ability of phagocytes
    E B de Oliveira-Junior
    Department of Immunology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil
    Scand J Immunol 73:420-7. 2011
    ..leading to classical CGD, and more recently discovered immunological defects secondarily affecting phagocyte respiratory burst function and resulting in primary immunodeficiencies with varied phenotypes, including susceptibilities to ..
  37. ncbi Changes in neutrophil functions in astronauts
    Indreshpal Kaur
    Enterprise Advisory Services Inc, Houston, TX 77058, USA
    Brain Behav Immun 18:443-50. 2004
    ..This study indicates that neutrophil phagocytic and oxidative functions are affected by factors associated with space flight and this relationship may depend on mission duration...
  38. ncbi Protein kinase C isoforms in neutrophil adhesion and activation
    Anna Bertram
    Department of Nephrology, Hannover Medical School, Carl Neuberg Strasse 1, Hannover, Germany
    Arch Immunol Ther Exp (Warsz) 59:79-87. 2011
    ..Protein kinase C (PKC)-δ is required for full assembly of NADPH oxidase and activation of the respiratory burst. Neutrophils also express PKC-α and -β, which may be involved in adhesion, degranulation and phagocytosis, ..
  39. pmc CD40 ligand promotes Mac-1 expression, leukocyte recruitment, and neointima formation after vascular injury
    Guohong Li
    Cardiovascular Division, Robert M Berne Cardiovascular Research Center, University of Virginia Health System, Charlottesville, VA, USA
    Am J Pathol 172:1141-52. 2008
    ....
  40. pmc The augmented neutrophil respiratory burst in response to Escherichia coli is reduced in liver cirrhosis during infection
    T Bruns
    Division of Gastroenterology, Hepatology and Infectious Disease, Department of Internal Medicine II, Jena University Hospital, Jena, Germany
    Clin Exp Immunol 164:346-56. 2011
    ..An increased resting respiratory burst has been observed in neutrophils from cirrhotic patients...
  41. ncbi Carbamylated albumin is a potent inhibitor of polymorphonuclear neutrophil respiratory burst
    Stéphane Jaisson
    Laboratory of Biochemistry and Molecular Biology, CNRS UMR 6198, Faculty of Medicine, University of Reims Champagne Ardenne, 51, rue Cognacq Jay, CNRS, F 51095 Reims Cedex, France
    FEBS Lett 581:1509-13. 2007
    ..Such an alteration of neutrophil oxidative functions might explain characteristic complications of renal failure, such as increased occurrence of inflammation or infections...
  42. ncbi Malaria circumsporozoite protein inhibits the respiratory burst in Kupffer cells
    Ivan Usynin
    Department of Medical Parasitology, New York University School of Medicine, 341 E 25 St, New York, NY 10010, USA
    Cell Microbiol 9:2610-28. 2007
    ..Here we show that Plasmodium sporozoites and recombinant circumsporozoite protein (CSP) suppress the respiratory burst in Kupffer cells...
  43. pmc The flavonoid luteolin inhibits Fcγ-dependent respiratory burst in granulocytes, but not skin blistering in a new model of pemphigoid in adult mice
    Eva Oswald
    Allergy Research Group, Department of Dermatology, University Freiburg Medical Center, Freiburg, Germany
    PLoS ONE 7:e31066. 2012
    ..Luteolin inhibited the Fcγ-dependent respiratory burst in immune complex-stimulated granulocytes and the autoantibody-induced dermal-epidermal separation in skin ..
  44. pmc Dihydroxyoctadecamonoenoate esters inhibit the neutrophil respiratory burst
    David Alan Thompson
    Department of Entomology and Cancer Research Center, University of California, Davis, CA 95616, USA
    J Biosci 32:279-91. 2007
    ..We evaluated whether the neutrophil respiratory burst,a surge of oxidant production thought to play an important role in limiting certain bacterial and fungal ..
  45. pmc p38MAPK, ERK and PI3K signaling pathways are involved in C5a-primed neutrophils for ANCA-mediated activation
    Jian Hao
    Renal Division, Key Laboratory of Renal Disease, Department of Medicine, Peking University First Hospital, Institute of Nephrology, Peking University, Ministry of Health of China, Beijing, China
    PLoS ONE 7:e38317. 2012
    ..The current study further investigated the signaling pathways of C5a-mediated priming of human neutrophils for ANCA-induced neutrophil activation...
  46. ncbi Anti-inflammatory thiazine alkaloids isolated from the New Zealand ascidian Aplidium sp.: inhibitors of the neutrophil respiratory burst in a model of gouty arthritis
    A Norrie Pearce
    TerraMarine Pharmaceuticals, Department of Chemistry, The University of Auckland, Private Bag 92019, Auckland, New Zealand
    J Nat Prod 70:936-40. 2007
    ..6 micromol/kg. Ascidiathiazone A (3) was synthesized in four steps from 8-hydroxyquinoline-2-carboxylic acid...
  47. ncbi Suppression of oxidative burst in human neutrophils with the naturally occurring serotonin derivative isomer from Leuzea carthamoides
    Radomir Nosal
    Institute of Experimental Pharmacology and Toxicology, Slovak Academy of Sciences, Bratislava, Slovakia
    Neuro Endocrinol Lett 31:69-72. 2010
    ..appropriately stimulated, they undergo dramatic physiological and biochemical changes resulting in phagocytosis, chemotaxis and degranulation with the activation of reactive oxygen species (ROS) production known as the respiratory burst.
  48. pmc Stimulation of the alveolar macrophage respiratory burst by ADP causes selective glutathionylation of protein tyrosine phosphatase 1B
    Alessandra Rinna
    School of Natural Sciences, University of California Merced, P O Box 2039, Merced, CA 95344, USA
    Free Radic Biol Med 41:86-91. 2006
    ..we show that, in the rat alveolar macrophage NR8383 cell line, H(2)O(2) produced through the ADP-stimulated respiratory burst induces the formation of a disulfide bond between PTP1B and GSH that was detectable with an antibody to ..
  49. ncbi Increased respiratory burst and increased expression of complement receptor-3 (CD11b/CD18) and of IL-8 receptor-A in neutrophil granulocytes from newborns after vaginal delivery
    Peter Gessler
    Intensive Care and Neonatology, University Children s Hospital, Berne, Institute of Immunology, University of Berne, Switzerland
    Biol Neonate 83:107-12. 2003
    ..The data demonstrate a higher functional responsiveness and a higher expression level of functionally important receptors in neutrophils after vaginal delivery possibly due to activation of neutrophil granulocytes during labor...
  50. ncbi Akt phosphorylates p47phox and mediates respiratory burst activity in human neutrophils
    Qingdan Chen
    Department of Medicine, University of Louisville, Louisville, KY 40202, USA
    J Immunol 170:5302-8. 2003
    b>Respiratory burst activity and phosphorylation of an NADPH oxidase component, p47(phox), during neutrophil stimulation are mediated by phosphatidylinositol 3-kinase (PI-3K) activation...
  51. ncbi Intracellular oxidative activity and respiratory burst of leukocytes isolated from multiple sclerosis patients
    G Ferretti
    Istituto di Biochimica, Facolta di Medicina e Chirurgia, Universita Politecnica delle Marche, Via Ranieri, 60131 Ancona, Italy
    Neurochem Int 48:87-92. 2006
    ..In our study, we compared intracellular oxidative activity and the respiratory burst activity in MS patients (n=20) and healthy controls (n=15) using leukocytes as cellular model...
  52. ncbi Development of a respiratory burst assay using zebrafish kidneys and embryos
    Andrea C Hermann
    Department of Biochemistry, Microbiology, and Molecular Biology, 5735 Hitchner Hall, University of Maine, Orono, ME 04469, USA
    J Immunol Methods 292:119-29. 2004
    ..of defense against invading pathogens and consists of a variety of immune defense mechanisms including the respiratory burst of phagocytes...
  53. ncbi Decreased activity of neutrophils in the presence of diferuloylmethane (curcumin) involves protein kinase C inhibition
    Viera Jancinova
    Institute of Experimental Pharmacology, Slovak Academy of Sciences, Dubravska cesta 9, 84104 Bratislava, Slovakia
    Eur J Pharmacol 612:161-6. 2009
    ..As neutrophils are considered to be cells with the greatest capacity to inflict damage within diseased joints, the observed effects could represent a further mechanism involved in the antirheumatic activity of diferuloylmethane...
  54. ncbi 2-Aminoethoxydiphenyl borate (2-APB) reduces respiratory burst, MMP-9 release and CD11b expression, and increases l-selectin shedding in bovine neutrophils
    I Conejeros
    Universidad Austral de Chile, Valdivia, Chile
    Res Vet Sci 92:103-10. 2012
    ....
  55. ncbi Cocoa flavanols and procyanidins can modulate the lipopolysaccharide activation of polymorphonuclear cells in vitro
    Thomas P Kenny
    Division of Rheumatology, Allergy and Clinical Immunology, School of Medicine, University of California, Davis, California 95616, USA
    J Med Food 12:1-7. 2009
    ....
  56. ncbi Free cholesterol alters lipid raft structure and function regulating neutrophil Ca2+ entry and respiratory burst: correlations with calcium channel raft trafficking
    Kolenkode B Kannan
    Department of Surgery, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA 02115, USA
    J Immunol 178:5253-61. 2007
    ..Responses to cholesterol appear physiologic because they regulate respiratory burst in a proportional biphasic fashion...
  57. ncbi The protective effects of HDL and its constituents against neutrophil respiratory burst activation by hypochlorite-oxidized LDL
    Steffi Kopprasch
    Department of Internal Medicine 3, Pathological Biochemistry, Carl Gustav Carus Medical School, University of Technology, Dresden, Germany
    Mol Cell Biochem 258:121-7. 2004
    ..low-density lipoprotein (oxLDL) possesses a substantial proinflammatory potential by modulating respiratory burst activities of polymorphonuclear neutrophils (PMN)...
  58. ncbi Induction of neutrophil responsiveness to myeloperoxidase antibodies by their exposure to supernatant of degranulated autologous neutrophils
    C Hess
    Department of Research, University Hospital Basel, Hebelstrasse 20, Basel, Switzerland
    Blood 96:2822-7. 2000
    ..This mechanism of dispersing PMN activation would be specific for MPO-ANCA and may explain differences in the pathologic and clinical expression of MPO-ANCA versus PR3-ANCA vasculitis. (Blood. 2000;96:2822-2827)..
  59. ncbi Increased polymorphonuclear leukocyte respiratory burst function in type 2 diabetes
    W Lee Hand
    Department of Internal Medicine, Texas Tech University Health Sciences Center, El Paso, TX 79905, USA
    Diabetes Res Clin Pract 76:44-50. 2007
    ..We evaluated oxidative respiratory burst activity (superoxide production) in non-stimulated and stimulated PMN from 70 stable type 2 Hispanic ..
  60. ncbi Role of mitogen-activated protein kinases in activation of human neutrophils by antineutrophil cytoplasmic antibodies
    R Kettritz
    Franz Volhard Clinic and Max Delbrück Center for Molecular Medicine, Medical Faculty of the Charite, Humboldt University of Berlin, Germany
    J Am Soc Nephrol 12:37-46. 2001
    ..b>Respiratory burst was measured with superoxide dismutase-inhibitable ferricytochrome C reduction and using dihydro-rhodamine-1,..
  61. ncbi Infection-induced respiratory burst in BALB/c macrophages kills Leishmania guyanensis amastigotes through apoptosis: possible involvement in resistance to cutaneous leishmaniasis
    Junia Sousa-Franco
    Departamento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, C P 486, 31270 901 Belo Horizonte, MG 30161 970, Brazil
    Microbes Infect 8:390-400. 2006
    ..This innate killing mechanism of macrophages may account for the resistance of BALB/c mice to infection by L. guyanensis...
  62. ncbi Post-160-km race illness rates and decreases in granulocyte respiratory burst and salivary IgA output are not countered by quercetin ingestion
    D Henson
    Department of Biology, Appalachian State University, Boone, North Carolina 28608, United States
    Int J Sports Med 29:856-63. 2008
    ..Significant pre- to postrace decreases were measured for natural killer cells (43 %), granulocyte respiratory burst activity (55 %), and salivary IgA output (48 %), and increases for neutrophil (288 %) and monocyte (211 %) ..
  63. ncbi Increased neutrophil respiratory burst in bcr-null mutants
    J W Voncken
    Department of Pathology, Childrens Hospital of Los Angeles 90027
    Cell 80:719-28. 1995
    ..These results connect Bcr in vivo with the regulation of Rac-mediated superoxide production by the NADPH-oxidase system of leukocytes and suggest a link between Bcr function and the cell type affected in Ph-positive leukemia...
  64. ncbi Effects of p38 mitogen-activated protein kinase inhibition on anti-neutrophil cytoplasmic autoantibody pathogenicity in vitro and in vivo
    Betty S van der Veen
    Department of Pathology and Medical Biology, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands
    Ann Rheum Dis 70:356-65. 2011
    ..To determine whether inhibition of p38 mitogen-activated protein kinase (p38MAPK) reduces the pathogenicity of anti-neutrophil cytoplasmic autoantibodies (ANCAs) in vitro and in vivo...
  65. ncbi Effects of flavonols on the generation of superoxide anion radicals by xanthine oxidase and stimulated neutrophils
    L Selloum
    Department of Biology, Faculty of Sciences, Setif, 19000, Algeria
    Arch Biochem Biophys 395:49-56. 2001
    ..Thus, an inhibition of enzymes involved in signaling rather than a scavenging of superoxide anion radicals dominates in fMet-Leu-Phe-stimulated neutrophils exposed to flavonols...
  66. pmc Simultaneous activation of NADPH oxidase-related proton and electron currents in human neutrophils
    T E Decoursey
    Departments of Molecular Biophysics and Physiology and Immunology Microbiology, Rush Presbyterian St Luke s Medical Center, Chicago, IL 60612, USA
    Proc Natl Acad Sci U S A 97:6885-9. 2000
    ..Phorbol myristate acetate (PMA) is a potent agonist for this "respiratory burst" in human neutrophils...
  67. ncbi Neutrophil respiratory burst is decreased in scleroderma and normalized by near-infrared mediated hyperthermia
    J Foerster
    Department of Dermatology, Charite Hospital, Berlin, Germany
    Clin Exp Dermatol 31:799-806. 2006
    ..The production of reactive oxygen species (ROS) by fibroblasts has been suggested to contribute to scleroderma pathogenesis. Infrared-mediated hyperthermia has recently been shown to be of benefit in scleroderma...
  68. pmc Importance of antibody and complement for oxidative burst and killing of invasive nontyphoidal Salmonella by blood cells in Africans
    Esther N Gondwe
    Malawi Liverpool Wellcome Trust Clinical Research Programme, College of Medicine, University of Malawi, Blantyre 3, Malawi
    Proc Natl Acad Sci U S A 107:3070-5. 2010
    ..These findings increase the likelihood that an antibody-inducing vaccine will protect against invasive nontyphoidal Salmonella disease in African children...
  69. ncbi Human neutrophil defensins increase neutrophil uptake of influenza A virus and bacteria and modify virus-induced respiratory burst responses
    Tesfaldet Tecle
    Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
    J Immunol 178:8046-52. 2007
    ..HNPs did not increase neutrophil respiratory burst responses to IAV...
  70. ncbi Signaling pathways for Fc gamma receptor-stimulated tumor necrosis factor-alpha secretion and respiratory burst in RAW 264.7 macrophages
    Daniel J Loegering
    Center for Cardiovascular Sciences, Albany Medical College, Albany, New York 12208, USA
    Inflammation 28:23-31. 2004
    ..Fc gammaR) signaling mediates several important macrophage functions including cytokine secretion and respiratory burst. The present study describes the development of a model using the macrophage cell line, RAW 264...
  71. ncbi C242T CYBA polymorphism of the NADPH oxidase is associated with reduced respiratory burst in human neutrophils
    Keith E Wyche
    Department of Medicine, Division of Cardiology, Emory University School of Medicine, Atlanta, GA 30322, USA
    Hypertension 43:1246-51. 2004
    ..05). In contrast, the A640G and the -930A/G polymorphisms did not alter the neutrophil respiratory burst. Phagocytic respiratory burst activity in homozygous individuals with the T allele of the C242T CYBA ..
  72. ncbi Assembly and activation of the neutrophil NADPH oxidase in granule membranes
    Anna Karlsson
    The Phagocyte Research Laboratory, Department of Rheumatology, University of Goteborg, S 413 46 Goteborg, Sweden
    Antioxid Redox Signal 4:49-60. 2002
    ..Signaling capabilities of the oxidants, affecting for example, the state of protein phosphorylation, regulation of transcription factors, and induction of apoptosis, are discussed...
  73. ncbi Protein kinase C zeta phosphorylates a subset of selective sites of the NADPH oxidase component p47phox and participates in formyl peptide-mediated neutrophil respiratory burst
    P M Dang
    Institut National de la Santé et de la Recherche Médicale Unité 479, Centre Hospitalier Universitaire Xavier Bichat, Paris, France
    J Immunol 166:1206-13. 2001
    ..In conclusion, p47(phox) is a substrate for PKC zeta and participates in the signaling cascade between fMLP receptors and NADPH oxidase activation...
  74. pmc Regulation of TNF-induced oxygen radical production in human neutrophils: role of delta-PKC
    Laurie E Kilpatrick
    Department of Physiology and Lung Center, Temple University School of Medicine, Philadelphia, Pennsylvania, USA
    J Leukoc Biol 87:153-64. 2010
    ..delta-PKC activates p47phox by serine phosphorylation or indirectly through control of ERK recruitment to p47phox...
  75. ncbi Activated protein C inhibits chemotaxis and interleukin-6 release by human neutrophils without affecting other neutrophil functions
    H F Galley
    Academic Unit of Anaesthesia and Intensive Care, School of Medicine, University of Aberdeen, Aberdeen, UK
    Br J Anaesth 100:815-9. 2008
    ..The effects of APC on inflammatory responses have also been reported. Neutrophils are key cells involved in early host defence mechanisms in sepsis. We hypothesized that APC may have effects on neutrophil function...
  76. ncbi Pathogen-induced, NADPH oxidase-derived reactive oxygen intermediates suppress spread of cell death in Arabidopsis thaliana
    Miguel Angel Torres
    Department of Biology, University of North Carolina, CB 3280, Coker Hall, Room 108, Chapel Hill, North Carolina 27599 3280, USA
    Nat Genet 37:1130-4. 2005
    ..Mutation of Arabidopsis thaliana respiratory burst oxidase (Atrboh) genes eliminated pathogen-induced ROI production but had only a modest effect on the ..
  77. pmc Francisella tularensis genes required for inhibition of the neutrophil respiratory burst and intramacrophage growth identified by random transposon mutagenesis of strain LVS
    Grant S Schulert
    Inflammation Program, University of Iowa, Iowa City, Iowa 52242, USA
    Infect Immun 77:1324-36. 2009
    ..Phagocytosis of the uracil auxotrophic mutants triggered a respiratory burst in neutrophils, and ingested bacteria were killed and fragmented in phagosomes that contained superoxide...
  78. ncbi A novel role for the beta 2 integrin CD11b/CD18 in neutrophil apoptosis: a homeostatic mechanism in inflammation
    A Coxon
    Department of Pathology, Brigham and Women s Hospital, Boston, Massachusetts, USA
    Immunity 5:653-66. 1996
    ..Thus, CD11b/CD18 plays a novel and unsuspected homeostatic role in inflammation by accelerating the programmed elimination of extravasated neutrophils...
  79. ncbi Resveratrol and curcumin reduce the respiratory burst of Chlamydia-primed THP-1 cells
    Ginette Deby-Dupont
    Center for Oxygen, Research and Development CORD, Institut de Chimie, B6a, University of Liege, Sart Tilman, Belgium
    Biochem Biophys Res Commun 333:21-7. 2005
    ..pneumonia infected monocytes (THP-1 cells) responded to stimulation by an increased respiratory burst linked to an increased NADPH oxidase (NOX) activity...
  80. ncbi Activation of mitogen-activated protein kinase cascades during priming of human neutrophils by TNF-alpha and GM-CSF
    K R McLeish
    Department of Medicine, University of Louisville Health Sciences Center, Veterans Affairs Medical Center, Kentucky, USA
    J Leukoc Biol 64:537-45. 1998
    ..Both ERK and p38 MAPK cascades contribute to the ability of TNF-alpha and GM-CSF to prime the respiratory burst response in human PMNs.
  81. ncbi Macrophage signaling and respiratory burst
    Karen E Iles
    Department of Environmental Health Sciences, School of Public Health, and Center for Free Radical Biology, University of Alabama at Birmingham, 35294 0022, USA
    Immunol Res 26:95-105. 2002
    ..Once the NADPH oxidase is activated, it produces superoxide and H2O2 in a process known as the respiratory burst. The involvement of O2...
  82. ncbi Positional identification of Ncf1 as a gene that regulates arthritis severity in rats
    Peter Olofsson
    Section for Medical Inflammation Research and Department of Cell and Molecular Biology, Solvegatan 19, I11 BMC, Lund University, S 22184 Lund, Sweden
    Nat Genet 33:25-32. 2003
    ..Hence, Ncf1 is associated with a new autoimmune mechanism leading to severe destructive arthritis, notably similar to rheumatoid arthritis in humans...
  83. pmc The role of neutrophils in the pathogenesis of transfusion-related acute lung injury
    Yoke Lin Fung
    Australian Red Cross Blood Service, Brisbane, Queensland, Australia
    Transfus Med Rev 23:266-83. 2009
    ..This article reviews TRALI pathophysiology, explores the role of the neutrophil, details practical information for appropriate diagnosis and promotes further studies into the pathogenesis of TRALI...
  84. ncbi Reversed Na+/Ca2+ exchange contributes to Ca2+ influx and respiratory burst in microglia
    Evan W Newell
    Toronto Western Research Institute, University of Toronto, Toronto, Ontario, Canada
    Channels (Austin) 1:366-76. 2007
    Phagocytosis and the ensuing NADPH-mediated respiratory burst are important aspects of microglial activation that require calcium ion (Ca(2+)) influx...
  85. ncbi Inhibition of the respiratory burst by resveratrol in human monocytes: correlation with inhibition of PI3K signaling
    Toryn M Poolman
    Pharmacology and Therapeutics Group, Department of Cardiovascular Sciences, University of Leicester, Clinical Sciences Building, Leicester Royal Infirmary, UK
    Free Radic Biol Med 39:118-32. 2005
    ..Other known inhibitors of both PI3K and Akt were also found to inhibit this response. Therefore, inhibition of signaling through the PI3K to NADPH oxidase by t-RVT might represent an important anti-inflammatory mechanism...
  86. ncbi Extracellular signal-regulated kinase inhibition by statins inhibits neutrophil activation by ANCA
    Mira Choi
    HELIOS Klinkum Berlin Franz Volhard Clinic and Max Delbrück Center for Molecular Medicine, Medical Faculty of the Charite, Humboldt University of Berlin, Berlin, Germany
    Kidney Int 63:96-106. 2003
    ..We tested the hypothesis that statins decrease respiratory burst activity of human polymorphonuclear neutrophils (PMN) in response to anti-neutrophil cytoplasmic antibodies (..
  87. ncbi Advanced glycation end products stimulate an enhanced neutrophil respiratory burst mediated through the activation of cytosolic phospholipase A2 and generation of arachidonic Acid
    Richard K M Wong
    Division of Medicine and Therapeutics, University of Leicester, Leicester Royal Infirmary, Leicester, UK
    Circulation 108:1858-64. 2003
    Advanced glycation end products (AGEs) enhance NADPH oxidase, and hence respiratory burst activity, of stimulated neutrophils...
  88. ncbi Respiratory burst in human neutrophils
    C Dahlgren
    The Phagocyte Research Laboratory, Department of Medical Microbiology and Immunology, University of Goteborg, Guldhedsgatan 10, S 413 46, Goteborg, Sweden
    J Immunol Methods 232:3-14. 1999
    ..The development of methodology to measure the generation/release of phagocyte respiratory burst products is thus of great importance, and a number of different techniques are currently in use for this ..
  89. pmc Francisella tularensis LVS evades killing by human neutrophils via inhibition of the respiratory burst and phagosome escape
    Ramona L McCaffrey
    University of Iowa, 2501 Crosspark Rd, MTF D154, Coralville, IA 52241, USA
    J Leukoc Biol 80:1224-30. 2006
    ..Moreover, evasion of intracellular killing can be explained, in part, by disruption of the respiratory burst. As judged by luminol-enhanced chemiluminescence and nitroblue tetrazolium staining, neutrophils infected ..
  90. ncbi Pre-existing glomerular immune complexes induce polymorphonuclear cell recruitment through an Fc receptor-dependent respiratory burst: potential role in the perpetuation of immune nephritis
    Yusuke Suzuki
    Renal and Vascular Research Laboratory, Fundacion Jimenez Diaz, Autonoma University, Madrid, Spain
    J Immunol 170:3243-53. 2003
    ..the induction of NF-kappa B and TNF-alpha much more than integrin (CD18) blockade, indicating a role for the respiratory burst before integrin-dependent accumulation...
  91. ncbi Effects of banding or burdizzo castration of bulls on neutrophil phagocytosis and respiratory burst, CD62-L expression, and serum interleukin-8 concentration
    W Y Pang
    Teagasc, Animal Bioscience Research Centre, Dunsany, Co Meath, Ireland
    J Anim Sci 87:3187-95. 2009
    ..144 h relative to treatment time, blood samples were collected for analyses of neutrophil phagocytosis and respiratory burst, neutrophil CD62-L expression, and serum IL-8 concentration...
  92. ncbi Early events in the perception of lipopolysaccharides in the brown alga Laminaria digitata include an oxidative burst and activation of fatty acid oxidation cascades
    Frithjof C Küpper
    Station Biologique, UMR 7139 CNRS UPMC and LIA DIAMS, BP 74, Roscoff, Brittany, France
    J Exp Bot 57:1991-9. 2006
    ..These results suggest that different signalling pathways are involved in the induction of the oxidative burst and oxylipin production...
  93. ncbi Elevated neutrophil respiratory burst activity in essential hypertensive patients
    Rajesh Ramasamy
    Immunology Laboratory, Department of Pathology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Malaysia
    Cell Immunol 263:230-4. 2010
    Neutrophils play a significant role in maintaining the integrity of innate immunity via their potent respiratory burst activity...
  94. ncbi Agonist and antagonist dual effect of the cross-linked S19 ribosomal protein dimer in the C5a receptor-mediated respiratory burst reaction of phagocytic leukocytes
    I Revollo
    Department of Molecular Pathology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 2 2 1 Honjo, Kumamoto, 860 0811, Japan
    Inflamm Res 54:82-90. 2005
    To examine the behavior of the crosslinked dimer of S19 ribosomal protein (RP S19), a natural C5a receptor ligand, in the C5a receptor-mediated respiratory burst reaction of monocytes and neutrophils.
  95. ncbi Vanillin suppresses Kupffer cell-related colloidal carbon-induced respiratory burst activity in isolated perfused rat liver: anti-inflammatory implications
    Jose E Galgani
    Department of Nutrition, Faculty of Medicine, University of Chile, Santiago, Chile
    Food Funct 3:1319-23. 2012
    ..increased the rate of O(2) consumption of the liver above basal values, an effect that represents the respiratory burst activity of Kupffer cells...
  96. ncbi Endocytosis of Xanthomonas campestris pathovar campestris lipopolysaccharides in non-host plant cells of Nicotiana tabacum
    Andrea Gross
    University Bielefeld, Faculty of Biology, Department of Genetics, POB 100131, D 33501 Bielefeld, Germany
    New Phytol 165:215-26. 2005
    ..These observations suggest specific endocytosis of LPS(X.c.c.) into tobacco cells. The possibility for a receptor-mediated endocytosis comparable to the mammalian system will be discussed...
  97. ncbi Activation of peroxisome proliferator-activated receptor gamma by nitric oxide in monocytes/macrophages down-regulates p47phox and attenuates the respiratory burst
    Andreas von Knethen
    Institute of Cell Biology, University of Kaiserslautern, Germany
    J Immunol 169:2619-26. 2002
    ..We conclude that NO participates in controlling the pro- vs anti-inflammatory phenotype of macrophages by modulating PPARgamma...
  98. ncbi An intact cytoskeleton is required for prolonged respiratory burst activity during neutrophil phagocytosis
    D Granfeldt
    Department of Medical Microbiology and Immunology, , Sweden
    Inflammation 25:165-9. 2001
    The temporal relationship between phagocytosis and respiratory burst activity was investigated...
  99. pmc Lipopolysaccharide-binding protein- and CD14-dependent activation of mitogen-activated protein kinase p38 by lipopolysaccharide in human neutrophils is associated with priming of respiratory burst
    Sen Rong Yan
    Department of Pediatrics, Dalhousie University and IWK Health Centre, Halifax, Nova Scotia, Canada
    Infect Immun 70:4068-74. 2002
    ..p38 was highly correlated with LPS priming of a formyl-methionyl-leucyl-phenylalanine (fMLP)-stimulated PMN respiratory burst. Treatment of PMN with the p38-specific inhibitor SB203580 significantly attenuated the respiratory burst in ..
  100. ncbi Activation of the neutrophil respiratory burst oxidase
    R A Clark
    Department of Medicine, University of Texas Health Science Center at San Antonio 78284 7870, USA
    J Infect Dis 179:S309-17. 1999
    The neutrophil respiratory burst oxidase is a multicomponent activatable enzyme comprising one of the major phagocyte antimicrobial systems...
  101. pmc Flavonoids inhibit the respiratory burst of neutrophils in mammals
    Milan Ciz
    Institute of Biophysics, Academy of Sciences of the Czech Republic, vvi, Brno, Czech Republic
    Oxid Med Cell Longev 2012:181295. 2012
    ..The present paper summarizes contemporary knowledge on the effects of various flavonoids on the respiratory burst of mammalian neutrophils...

Research Grants64

  1. Effect of PPARy Ligands on Alcohol-Induced Alveolar Macrophage Oxidative Stress
    Samantha M Yeligar; Fiscal Year: 2013
    ..Nox) 1, Nox2, and Nox4 are critical sources of reactive oxygen species (ROS), and Nox2 is essential for the respiratory burst involved in killing microbes after phagocytosis. However, excessive ROS production suppresses phagocytosis...
  2. Systemic Inflammation and Central Nervous System Dysfunction
    Laura L Dugan; Fiscal Year: 2013
    ..expression and activation of NADPH oxidase (Nox2), a multimeric enzyme complex first described as the respiratory burst oxidase in neutrophils...
  3. Redox regulation of phagocyte NOX2 in inflammation and aging
    Robert A Clark; Fiscal Year: 2013
    ..This respiratory burst oxidase is genetically absent in patients with chronic granulomatous disease, resulting in recurring severe ..
  4. Free Radicals and Methamphetamine Abuse
    Maria Cecilia Garibaldi Marcondes; Fiscal Year: 2010
    ..These approaches will help dissect the molecular basis of respiratory burst induction by Meth, and will distinguish whether Meth interferes over mitochodria, peroxisome, or both...
  5. Structure and function analysis of C1/C1q and MBL using a novel peptide inhibitor
    NEEL KUMAR KRISHNA; Fiscal Year: 2012
    ..utilizing flow cytometry and (b) characterizing the biological consequence of CPP disruption of the C1q-CRT/cC1qR interaction on respiratory burst activity in neutrophils using a SOD-inhibitable ferricytochrome C reduction assay.
  6. Molecular Basis for Priming of the Neutrophil NADPH Oxidase in Trauma and Sepsis
    ALBERT T HSU; Fiscal Year: 2010
    ..The neutrophil NADPH oxidase molecule is the respiratory burst enzyme that produces the toxic ROS during the inflammatory response...
  7. Non-oxidative Resistance to A. fumigatus by Alveolar Macrophages
    James B Burritt; Fiscal Year: 2010
    ..role of NADPH oxidase of AM in protecting humans from IPA are conflicting, and our data do not indicate the respiratory burst in AM is necessary for a resistance to aspergillosis, as we have shown it is in neutrophils...
  8. Eosinophil Peroxidase in Allergic Inflammation
    Arne Slungaard; Fiscal Year: 2012
    ..EO specific granules are endowed with abundant amounts of EO peroxidase (EPO) and a vigorous respiratory burst that generates H2O2 to fuel the generation of other oxidants...
  9. Identification of novel DNA repair mechanisms in Mycobacterium tuberculosis
    Pallavi Ghosh; Fiscal Year: 2012
    ..This primarily constitutes the respiratory burst that occurs upon phagocytosis of the bacteria by alveolar macrophages and results in the generation of ..
  10. IMMUNOBIOLOGY OF SCHISTOSOME-MOLLUSC INTERACTIONS
    Timothy P Yoshino; Fiscal Year: 2013
    ..kinases (Erks, p38) and a Rac-like GTPase in signaling immune effector responses, particularly induction of respiratory burst activity (H2O2 production), in S and R snail hemocytes upon specific CHO stimulation, and (4) explore the ..
  11. Interactions Between Anaplasma Phagocytophilum and Ixodes Scapularis
    Erol Fikrig; Fiscal Year: 2013
    ..requires fucosylation of host proteins for infection, and represses gp91phox in neutrophils to prevent the respiratory burst. In ticks Anaplasma induces I...
  12. UNDERLYING MECHANISMS OF SCHISTOSOME/SNAIL COMPATIBILITY
    CHRISTOPHER JEFFREY BAYNE; Fiscal Year: 2012
    ..In particular, the research deals with genes, messenger RNAs and proteins involved in the cellular respiratory burst - a complex pathway in which damaging reactive oxygen and nitrogen species are produced by the defense cells ..
  13. The Role of Na+/H+ exchanger in Cerebral Ischemia
    Dandan Sun; Fiscal Year: 2013
    ..Secondly, activated microglia produce a "respiratory burst" via NADPH oxidase...
  14. Beta2 Integrin Activation and Signaling
    Vineet Gupta; Fiscal Year: 2010
    ..mediate the divalent metal ion dependent adhesion of leukocytes including homing, firm adhesion, migration, respiratory burst and clearance of pathogens through phagocytosis and cell-mediated killing, beta2 integrins also contribute ..
  15. Role of C/EBPepsilon in myeloid differentiation
    Stephanie Halene; Fiscal Year: 2010
    ..The neutrophils have bilobed nuclei, abnormal respiratory burst activity, and impaired chemotaxis and bactericidal activity...
  16. Molecular mechanisms of voltage-gated proton channels
    Hans Peter Larsson; Fiscal Year: 2013
    ..cells: HV channels in macrophages are essential for the generation of reactive oxygen species during the respiratory burst, which is critical to the process of phagocytosis and the destruction of foreign pathogens...
  17. GENETIC RESPONSE TO OXIDATIVE STREET IN E COLI
    SPENCER FARR; Fiscal Year: 1993
    ..Besides normal metabolism, other sources of oxygen radicals include: ionizing radiation, Near UV, respiratory burst oxidase in phagocytes and neutophiles, drugs (i.e...
  18. Monocyte subsets and host defense
    David M Mosser; Fiscal Year: 2012
    ..We propose to examine the mechanism(s) of monocyte killing, and examine the role of the monocyte respiratory burst (NOX2) in parasite killing...
  19. REGULATION OF OXYGEN METABOLISM IN BLOOD NEUTROPHILS
    LINDA MCPHAIL; Fiscal Year: 2005
    The phagocyte respiratory burst enzyme, NADPH oxidase, produces superoxide anion...
  20. Peroxidase-Based Toxicity In Hypereosinophilic States.'
    Arne Slungaard; Fiscal Year: 2006
    ..Despite the abundance of EPO and the vigor of the EO respiratory burst, little is known about the contribution of EPO-mediated oxidative damage to the pathology of eosinophilic ..
  21. Nox Isoforms and Vascular Cell Phenotype
    John Keaney; Fiscal Year: 2009
    ..Principle among these are the NADPH oxidase family of enzymes that are related to the classical neutrophil respiratory burst oxidase...
  22. METALLOTHIONEIN, MERCURY AND MONOCYTE ACTIVATION
    RUDOLFS ZALUPS; Fiscal Year: 2006
    ..Activation is the acute phase response and respiratory burst undergone by monocytes (and other leukocytes) as a response to extracellular events and signals...
  23. METABOLIC ACTIVATION OF ARYLAMINES IN LEUKOCYTES
    Michael Corbett; Fiscal Year: 1991
    ..cells is already known to result from the release of reactive oxygen species following the normal "respiratory burst" of such cells...
  24. Treatment of Anthrax with Nonantimicrobial Tetracyclines
    Sanford Simon; Fiscal Year: 2003
    ..of ARDS, administration of CMT-3 prevents the otherwise fatal progression of lung damage while leukocyte respiratory burst activity, leukocyte infiltration of the lung alveolar spaces, and levels of leukocyte-derived proteases are ..
  25. B DERMATITIDIS CONIDIAL-MACROPHAGE INTERACTIONS
    Stephen Moser; Fiscal Year: 1993
    ..including production of interleukin-I (IL-1), tumor necrosis factor (TNF), membrane Ia expression and respiratory burst following exposure to conidia: and (3) to determine the fate of conidia phagocytized by alveolar macrophages...
  26. TREATMENT OF ARDS WITH FRUCTOSE DIPHOSPHATE
    ANGEL MARKOV; Fiscal Year: 1991
    ..FDP prevents or attenuates formation of non-cardiogenic edema is that this agent inhibits completely the "respiratory burst" in stimulated neutrophils...
  27. CELL DIVISION AND SURFACE GROWTH IN A MODEL SYSTEM
    Michael Higgins; Fiscal Year: 1992
    Stimulation of human neutrophils induces a "respiratory burst" causing production of 02- and H202...
  28. TOXIC ALDEHYDES AS MODULATORS OF LUNG INFLAMMATION
    Albert van Der Vliet; Fiscal Year: 2007
    ..shown to alter several neutrophil properties, including stimulation of cytokine release, inhibition of the respiratory burst, and interference with constitutive neutrophil apoptosis...
  29. EX-VIVO RESPIRATORY BURST RESPONSE TO A. FUMIGATUS AFTER STEM CELL TRANSPLANT
    Charulata Ramaprasad; Fiscal Year: 2009
    ..Specific Aim #1: To characterize pathogen-specific neutrophil respiratory burst responses after allogeneic stem cell transplantation...
  30. HEMOLYSIS AND PHAGOCYTIC DYSFUNCTION DURING SHOCK
    Daniel Loegering; Fiscal Year: 1991
    ..2) To determine if the phagocytosis of erythrocytes depresses the in vitro respiratory burst response to soluble and phagocytic stimuli in macrophages...
  31. HOW DOES RAC CONTROL HUMAN PHAGOCYTE NADPH OXIDASE?
    Paul Heyworth; Fiscal Year: 1999
    DESCRIPTION: In response to a variety of stimuli, phagocytic cells undergo a respiratory burst, catalyzed by an NADPH oxidase complex that reduces oxygen to (superoxide is O2-)...
  32. Protein Kinase C in Macrophage Activation
    MICHELLE LENNARTZ; Fiscal Year: 2006
    ..PKC plays a central role in FcgammaR-mediated signaling, mediating phagocytosis, respiratory burst, and cytokine production...
  33. INTERACTIONS OF CRP WITH HUMAN MONOCYTES AND NEUTROPHILS
    Janice Zeller; Fiscal Year: 1990
    ..protein (CRP), an acute phase reactant, binds to human monocytes and neutrophils and enhances their respiratory burst response to IgG...
  34. PATHOPHYSIOLOGY AND METABOLISM OF THE LEUKOCYTE
    David Bass; Fiscal Year: 1992
    ..in two sequellae: the mobilization and subsequent metabolism of arachidonic acid and activation of the respiratory burst. The role of Ca++ will be investigated by comparing Ca++ transport induced by various stimuli with ..
  35. UNDERLYING MECHANISMS OF SCHISTOSOME/SNAIL COMPATIBILITY
    Christopher Bayne; Fiscal Year: 2001
    ..The PI hypothesizes that: (a) there can be strain-specific differences in the respiratory burst of hemocytes; (b) that sensitivity differences exist in the S and R hemocytes to negative influence of a ..
  36. HTS Assay for Discovery:Small Molecule Regulators (RMI)
    Vineet Gupta; Fiscal Year: 2005
    ..mediate the divalent metal ion dependent adhesion of leukocytes including homing, firm adhesion, migration, respiratory burst and clearance of pathogens through phagocytosis and cell mediated killing...
  37. Regulation of Myeloperoxidase Catalysis by Nitric Oxide
    HUSAM ABU SOUD; Fiscal Year: 2004
    ..both the extracellular milieu and the phagolysosome where it uses hydrogen peroxide (H2O2) produced during a respiratory burst as co-substrate...
  38. STRUCTURE/FUNCTION ANALYSIS OF PHAGOCYTE PROTEINS
    Mary Dinauer; Fiscal Year: 2009
    The phagocyte respiratory burst oxidase that generates the superoxide radical plays a central role in host defense and the inflammatory response...
  39. Reactive oxygen species in alveolar macrophage signaling
    HENRY FORMAN; Fiscal Year: 2004
    H2O2 produced by the respiratory burst of alveolar macrophages participates in autocrine signaling for activation of the protein kinases, ERKI and ERK2, and the transcription factor, AP-1...
  40. LEUKOCYTE INTEGRIN ALPHA M BETA 2
    Edward Plow; Fiscal Year: 2004
    ..A number of specific leukocyte responses are regulated by alphaMbeta2, including the respiratory burst, homotypic aggregation, phagocytosis, migration, apoptosis, tumor surveillance, and ischemia-reperfusion ..
  41. HoxA10 Function During Myeloid Differentiation
    Elizabeth Eklund; Fiscal Year: 2006
    ..We noted that repressor elements in the promoters of the genes encoding the respiratory burst oxidase components gp91phox and p67phox (the CYBB and NCF2 genes) are similar to the derived DNA-binding ..
  42. PRIMING & STIMULATION OF GRANULOCYTES
    David Bass; Fiscal Year: 1992
    Priming" of granulocytes (PMNL) signifies an increased responsiveness to stimulation in vitro. The respiratory burst is important to the bactericidal activity of PMNL and priming of the burst may be an important aspect of host defense ..
  43. CHEMOKINE INDUCTION BY RECEPTOR MEDIATED PHAGOCYTOSIS
    Joseph Paulauskis; Fiscal Year: 1999
    ..Binding of opsonized particles by cell surface receptors of macrophages results in a substantial respiratory burst and subsequently increases in mRNA transcripts for pro-inflammatory cytokines including the Platelet Factor-..
  44. Neutrophil gene expression induced by A. phagocytophila
    Erol Fikrig; Fiscal Year: 2004
    ..Our recently published report demonstrates that A. phagocytophila inhibits the respiratory burst by selectively down-regulating gp91 phox, a major subunit of the NADPH oxidase holoenzyme...
  45. OXYGEN MUTAGENESIS IN HUMAN SOMATIC CELLS
    Raymond Monnat; Fiscal Year: 1992
    ..Our specific aims are: 1) We will determine the mutagenicity of the respiratory burst of granulocytes and of superoxide anion generated in vitro in a forward mutation assay system we have ..
  46. Signaling by Cytoplasmic Tyrosine Kinases in Leukocytes
    Clifford Lowell; Fiscal Year: 2005
    ..Neutrophils lacking Hck and Fgr fail to undergo cell spreading, respiratory burst or granule exocytosis following plating on surfaces which crosslink beta2 or beta3 integrins...
  47. PRODUCTION OF TOXIC OXYGEN SPECIES IN ACUTE LUNG INJURY
    ALFRED TAUBER; Fiscal Year: 1992
    The elucidation of the mechanism by which the human neutrophil mounts the non-mitochondrial respiratory burst is crucial in understanding neutrophil-mediated lung damage and normal microbicidal function...
  48. ALTERED LEUKOTRIENE RELEASE IN PARASITIZED PHAGOCYTES
    William Henderson; Fiscal Year: 1990
    ..gondii is an obligate intracellular protozoal parasite that actively invades macrophages without triggering respiratory burst activity, prevents the fusion of the phagosome (containing the ingested parasite) with lysosomes and ..
  49. MECHANISMS OF RESISTANCE TO BACTERIAL INFECTION
    Richard Johnston; Fiscal Year: 1993
    ..projects proposed here seek to gain knowledge about the molecular nature of the events that comprise the "respiratory burst system": binding of the stimulus (organism) to the phagocyte membrane ->initiation and transduction of a ..
  50. TRAUMA, INFECTION, AND PROSTHETIC BIOMATERIALS
    Richard Simmons; Fiscal Year: 2000
    ..We have carefully explored, in the previous grant period, the direct interactions between neutrophilic respiratory burst and various biomaterials...
  51. REGULATION OF SUPEROXIDE DISMUTASES IN THE LUNG
    Harry Nick; Fiscal Year: 2006
    ..of oxygen toxicity" links the deleterious pulmonary effects of hyperoxia, cellular oxygen metabolism and the respiratory burst of activated inflammatory cells to highly reactive metabolic products of oxygen...
  52. VOLTAGE-GATED PROTON CHANNELS IN HUMAN NEUTROPHILS
    THOMAS DECOURSEY; Fiscal Year: 2008
    ..5) We will identify the proton channel molecule and gene. ..
  53. VOLTAGE-GATED PROTON CHANNELS IN ALVEOLAR EPITHELIUM
    THOMAS DECOURSEY; Fiscal Year: 2003
    ..They appear to have been designed to extrude acid from cells. In phagocytes they are activated during the respiratory burst, the process of killing bacteria, thus they facilitate the inflammatory response...
  54. INTERACTION OF HELICOBACTER PYLORI WITH PHAGOCYTES
    LEE ANN ALLEN; Fiscal Year: 2003
    ..A complete dissection of this process at the molecular level may lead to novel therapies for treatment of H. pylori infection and reduce the significant morbidity associated with ulcer disease. ..
  55. Role of CIC-3 channels in regulation of cell volume and shape in neutrophils
    Jessica Moreland; Fiscal Year: 2007
    ..The studies proposed will advance our fundamental knowledge of the function of neutrophils in the maintenance of normal immune system function. [unreadable] [unreadable] [unreadable]..
  56. NEUTROPHIL RECRUITMENT IN LPS-INDUCED AIRWAY DISEASE
    Jessica Moreland; Fiscal Year: 2004
    ....
  57. Interaction of H. capsulatum with Dendritic Cells
    Simon Newman; Fiscal Year: 2006
    ..The results of these studies should provide significant insight into the pathogenesis of histoplasmosis and aid in the design of novel vaccine strategies for the prevention of disease. ..
  58. REGULATION OF CHEMOATTRACTANT ACTIVATION OF NEUTROPHILS
    ALGIRDAS JESAITIS; Fiscal Year: 2003
    ....
  59. Coupling & Reconfiguration of Rhythmic Motor Networks
    Stephen Johnson; Fiscal Year: 2007
    ..unreadable] [unreadable] [unreadable]..
  60. Prenatal TCDD and postnatal autoimmune disease
    Steven Holladay; Fiscal Year: 2007
    ..The collective experiments are designed to detect alterations in fetal immune development caused by TCDD that underlie the worsened postnatal (postpubertal) autoimmune disease caused by this chemical. ..
  61. ALTERED NEUTROPHIL APOPTOSIS /BRONCHOPULMONARY DYSPLASIA
    Barry Weinberger; Fiscal Year: 2006
    ..The identification of specific pathways mediating apoptosis that are altered in neonatal neutrophils may also suggest preventive therapeutic strategies. ..
  62. IGG PLACENTAL TRANSPORT: ENDOTHELIAL CAVEOLAE
    John Robinson; Fiscal Year: 2004
    ..Our approaches to testing the predictions of this hypothesis incorporate methods of cell biology, immunology, biochemistry, ultrastructural analysis, and molecular biology. ..
  63. PLASTICITY OF SPINAL RESPIRATORY SYNAPTIC TRANSMISSION
    Stephen Johnson; Fiscal Year: 2001
    ....
  64. TGF-BETA, CHLORIDE CHANNELS AND APOPTOSIS OF AIRWAY EPITHELIAL CELLS
    Devendra K Agrawal; Fiscal Year: 2010
    ..Such investigations would provide unique insights to the pathophysiologic process of chronic asthma and the means to prevent or reverse the disease. ..